CN101088362A - Fish and shrimp phagostimulant of famented product adhesion protein and its prepn process - Google Patents
Fish and shrimp phagostimulant of famented product adhesion protein and its prepn process Download PDFInfo
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- CN101088362A CN101088362A CNA200610027638XA CN200610027638A CN101088362A CN 101088362 A CN101088362 A CN 101088362A CN A200610027638X A CNA200610027638X A CN A200610027638XA CN 200610027638 A CN200610027638 A CN 200610027638A CN 101088362 A CN101088362 A CN 101088362A
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- fish
- bifidobacterium
- phagostimulant
- lactobacillus casei
- shrimp
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- 239000001963 growth medium Substances 0.000 claims abstract description 16
- 238000001035 drying Methods 0.000 claims abstract description 14
- 238000005516 engineering process Methods 0.000 claims abstract description 7
- 238000012216 screening Methods 0.000 claims abstract description 4
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims description 30
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- 235000013958 Lactobacillus casei Nutrition 0.000 claims description 18
- 238000000855 fermentation Methods 0.000 claims description 18
- 230000004151 fermentation Effects 0.000 claims description 18
- 229940017800 lactobacillus casei Drugs 0.000 claims description 18
- 241000222120 Candida <Saccharomycetales> Species 0.000 claims description 17
- 230000001580 bacterial effect Effects 0.000 claims description 15
- 239000004310 lactic acid Substances 0.000 claims description 15
- 235000014655 lactic acid Nutrition 0.000 claims description 15
- 238000002360 preparation method Methods 0.000 claims description 14
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- 239000012141 concentrate Substances 0.000 claims description 12
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- 229920001817 Agar Polymers 0.000 claims description 9
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- BNIILDVGGAEEIG-UHFFFAOYSA-L disodium hydrogen phosphate Chemical compound [Na+].[Na+].OP([O-])([O-])=O BNIILDVGGAEEIG-UHFFFAOYSA-L 0.000 claims description 2
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- GVALZJMUIHGIMD-UHFFFAOYSA-H magnesium phosphate Chemical compound [Mg+2].[Mg+2].[Mg+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O GVALZJMUIHGIMD-UHFFFAOYSA-H 0.000 claims description 2
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- KWIUHFFTVRNATP-UHFFFAOYSA-N Betaine Natural products C[N+](C)(C)CC([O-])=O KWIUHFFTVRNATP-UHFFFAOYSA-N 0.000 description 3
- KWIUHFFTVRNATP-UHFFFAOYSA-O N,N,N-trimethylglycinium Chemical compound C[N+](C)(C)CC(O)=O KWIUHFFTVRNATP-UHFFFAOYSA-O 0.000 description 3
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- AZJQQNWSSLCLJN-UHFFFAOYSA-N 2-ethoxyquinoline Chemical compound C1=CC=CC2=NC(OCC)=CC=C21 AZJQQNWSSLCLJN-UHFFFAOYSA-N 0.000 description 1
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- GXCLVBGFBYZDAG-UHFFFAOYSA-N N-[2-(1H-indol-3-yl)ethyl]-N-methylprop-2-en-1-amine Chemical compound CN(CCC1=CNC2=C1C=CC=C2)CC=C GXCLVBGFBYZDAG-UHFFFAOYSA-N 0.000 description 1
- 241000157468 Reinhardtius hippoglossoides Species 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
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- 239000000853 adhesive Substances 0.000 description 1
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- 235000021120 animal protein Nutrition 0.000 description 1
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- ACTIUHUUMQJHFO-UPTCCGCDSA-N coenzyme Q10 Chemical compound COC1=C(OC)C(=O)C(C\C=C(/C)CC\C=C(/C)CC\C=C(/C)CC\C=C(/C)CC\C=C(/C)CC\C=C(/C)CC\C=C(/C)CC\C=C(/C)CC\C=C(/C)CCC=C(C)C)=C(C)C1=O ACTIUHUUMQJHFO-UPTCCGCDSA-N 0.000 description 1
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Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
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- Fodder In General (AREA)
Abstract
The fish and shrimp phagostimulant of fermented product and adsorbed protein is prepared through screening beneficial bacteria seed from cultivated animal body, culturing in composite culture medium to proliferate, concentrating and adding plant protein with phagostimulant effect. It is added into fish and shrimp feed to raise their feed intake and improve their digesting function. The production process has combined beneficial microbe fermenting technology and low temperature drying technology to ensure the fast growth of beneficial bacteria, the well preservation of metabolic product and the special cultivating function. The fish and shrimp phagostimulant is one kind of green product capable of replacing traditional phagostimulant and antioxidant.
Description
Technical field
The present invention relates to fish and shrimp phagostimulant and preparation method that a kind of tunning adhesion protein is produced, by in fish feed, adding, the peat-reek and the profitable strain that utilize microbial fermentation to produce improve aquatic livestock enteron aisle and digestive function, improve feed intake and the daily gain of animal, belong to the agro-ecology product technique field.
Background technology
Holding time for pursuing the fastest culture efficiency and improving feed in the aquaculture now, antioxidants such as a large amount of amino acid, betaine and ethoxyquinoline in feed, have been added, objectively reached the effect that improves cultured output and food calling, but make feed produce strong peculiar smell simultaneously, and betaine and antioxidant all are Chemical Manufacture at present, enrichment in culturing the fish body is for breeding production has been buried serious potential safety hazard.
At present, disclosed patent, publication number is CN92111616.0, be entitled as the mussel protein powder that Production by Microorganism Fermentation has the prawn luring function, it is as carrier with various plants albumen, animal mussel slurry is continuously fermented through two warm solids by multiple microorganism as the important protein source, produces a kind of animal protein feed.Be mainly used to do phagostimulant and the protein source of aquatic products (prawn, fish) and poultry, domestic animal (chicken, ox etc.).What it was selected for use is special high protein culture medium and fermentate, does not carry out high-temperature sterilization and microbiological indicator detection simultaneously in the sweat, and the animal safety of its production is difficult to guarantee.
Summary of the invention
The purpose of this invention is to provide fish and shrimp phagostimulant and preparation method that the tunning adhesion protein is produced, can substitute the use of betaine, antioxidant in the feed fully, the peat-reek that utilizes microbial fermentation to produce, reach the effect of food calling, adsorb special vegetable protein simultaneously, when the nutrition of breed fish body is provided, cover the peculiar smell in the feed, reach the culture efficiency of green safety.
For realizing above purpose, technical scheme of the present invention provides the fish and shrimp phagostimulant that a kind of tunning adhesion protein is produced, it is characterized in that, formulated by following percentage by weight: Lactobacillus casei 30-40%, Bifidobacterium 30-40%, false gastral cavity Candida 20-40%.
The preparation method of the fish and shrimp phagostimulant that a kind of tunning adhesion protein is produced is characterized in that, adopts fluid enlargement culture, adopts cryogenic separation and dry technology in concentration process, adds vegetable protein simultaneously in concentrating, and its preparation process is:
The first step: the separation of probiotics, purifying and preparation
A. separate: getting chyme in the crucian enteron aisle of health is the bacterium source, utilize the lactic acid bacteria culture medium to carry out strain separating, be separated to two kinds of lactic acid bacterias, the lactic acid bacteria that this product adopts has two kinds, for: Lactobacillus casei and licheniformis (13123), Bifidobacterium (Bifidobacterium ATCC7469), utilize yeast extract wort agar culture medium to separate a kind of saccharomycete in the prawn enteron aisle, the saccharomycete that the present invention adopts is maternity leave gastral cavity Candida (Candida utilis CBCC5638);
B. purifying: the Lactobacillus casei that separation is obtained adopts the lactic acid bacteria culture medium to rule, separate with licheniformis, Bifidobacterium (Bifidobacterium), and false gastral cavity Candida (Candida utilis) utilizes yeast extract wort agar culture medium to rule, separate;
C. the production production of hybrid seeds: the bacterial classification inoculation of purifying to the eggplant bottle that fills nutrient agar, was cultivated 28-30 hour under 30-35 ℃ of temperature, observed when lawn is yellow-white and is covered with eggplant bottle inclined-plane in the eggplant bottle, put into 4 ℃ of refrigerators and keep;
Second step: the Liquid Culture of bacterial classification
A. take by weighing Lactobacillus casei, Bifidobacterium and the false gastral cavity Candida of the step 1 production production of hybrid seeds by weight ratio, inoculating proportion of the present invention is: Lactobacillus casei 30-40%, Bifidobacterium 30-40%, false gastral cavity Candida 20-40%;
B. with the Lactobacillus casei that takes by weighing, Bifidobacterium, false gastral cavity Candida in 1: the ratio of 20-33 is then in 120-130 ℃, the complex medium of 30-40min sterilization, put into fermentation tank then and stir 30min, its rotating speed is 200r/min, putting into sterilization back Plastic Drum after stirring left standstill 10-13 days, leave standstill in the process and to measure the Ph value every day, fermentation ends when Ph arrives 3.0-4.0;
The 3rd step: concentrate absorption with vegetable protein
A. after the fermentation ends, being the screw settling centrifuge of 2700-3200r/min by rotating speed earlier with zymotic fluid, is that the butterfly centrifugal machine of 6800-7300r/min concentrates by rotating speed again, and concentration time is 4-8 hour;
B. concentrate the back and collect centrifugal thing, add corn protein powder in the 1-3% ratio, the mixture after the interpolation stirs in conical agitator, and control moisture is at 50-65%;
The 4th step: dry and pulverizing
A. adopt the Clothoid type drying machine, Steam Heating is carried out drying, the control temperature is at 40-45 ℃, time 60-80min in drying, moisture is detected in the dry back of finishing, require dried material moisture≤12%, color and luster is faint yellow or yellowish-brown, makes dry deposits yields Maillard reaction, and smell fragrance is strong;
B. crushing screening: dried material is pulverized on pulverizer, and the material after the pulverizing is crossed 50 eye mesh screens, packs into behind the sieve to have in the bucket or bag of inner bag, tightens sack, fastens Sign Board, changes warehouse for finished product over to and stacks in order;
Described complex medium is at least three kinds of mixtures that sterilized water adds bean cake powder, urea, clear chaff, ammonium sulfate, dusty yeast, corn flour, molasses, potassium dihydrogen phosphate, sodium hydrogen phosphate, magnesium phosphate, magnesium sulfate again, and its weight percent proportioning is: bean cake powder 2-5%, molasses 9-15%, dusty yeast 2-5%, potassium dihydrogen phosphate 0-0.6%, sodium dihydrogen phosphate 0.5-0.8%, magnesium sulfate 0-0.1%, sterilized water 73.6-85.5%.
The bacterial classification that the present invention adopts mainly is lactic acid bacteria and saccharomycete, normal flora has material impact to the health of its body in the cultivated animals body, microecological balance between they and host has guaranteed the eubolism of host animal, improved host's immune disease-resistance power, also abundant nutriments such as vitamin are provided simultaneously for growing of host, therefore can improve microbial population of animal intestinal tract, promote that the probiotics preparation of animal health growth is generally paid attention to, development is rapid, and it has mainly acted on:
(1) suppresses the harmful intestinal tract microorganism, after probio enters alimentary canal, a large amount of breedings, in alimentary canal, form dominant microflora, thereby suppress harmful microbe propagation, Olsson (1992) confirms can suppress Vibrio anguillarum from the isolated bacterium of turbot enteron aisle with experiment in vitro, and it has stronger adhesive capacity than Vibrio anguillarum in enteron aisle mucus;
(2) supplement the nutrients, improve organism metabolism, reduce feed coefficient, probiotics preparation itself as additive just contains a large number of nutrients, as multiple proteins, vitamin, trace element, ubiquinone etc., probio also can produce many digestive enzymeses simultaneously, assists feed digestion, improves feed efficiency;
(3) stimulating immune system improves immunity, is good immunoactivator as the probiotics preparation of feed addictive, excites immunologic function by producing the nospecific immunity regulatory factor, strengthens immunity of organisms and premunition;
(4) bacterial classification of the present invention in liquid fermentation, produce metabolism products such as the profitable strain of high concentration and enzyme, vitamin, polyphenoils enter culture the fish body after, can in fish body enteron aisle, breed rapidly, improve fish body microecological balance, improve the human body immunocompetence, realize the breed purpose of green safety.
The phagostimulant that this patent product can be used as aquiculture animal adds, its addition is 1: 800-1000, bacterial classification in this patent product all from the aquatic livestock enteron aisle seed selection cultivate and to form, for feedstuff industry 813 bulletins allow to add bacterial classification, pass through fluid enlargement culture, produce profitable strain and a large amount of metabolite of high concentration, in concentration process, adopt cryogenic separation and dry technology, profitable strain and enzyme have been guaranteed, amino acid, coenzyme, vitamin is fully preserved, in concentrating, add special vegetable protein simultaneously, nutrient protein in the palatability of raising product and the supplement feed.
The invention has the advantages that:
1. adopt beneficial microbe fermentation technique and low temperature drying technology to combine, both guaranteed the high-speed rapid growth of probiotics, guaranteed that again its metabolite keeps fully, guaranteed that to greatest extent product is to culturing the special efficacy of body;
2, used bacterial classification all screens from cultivated animals, guarantees its metabolism security.Lactic acid bacteria is cultured body enteron aisle propagation, keeps the animal body microecological balance, regulates body immunity, and saccharomycete decomposes feed, produces a large amount of single cell proteins, satisfies and cultures the bulk-growth needs;
3, the special fermenting aroma food calling that utilizes microbial fermentation and produced, fragrant odour is strong, lasting, does not need to add phagostimulant and antioxidant, has guaranteed the food security in the breeding process, has reduced aquaculture cost;
4, by adding special vegetable protein, increase the food calling effect of product, replenished the nutrient protein of feed simultaneously;
5, production process is few, and not high to equipment and technological requirement, use amount is few, is easy to promote in aquaculture.
The specific embodiment
The invention will be further described below in conjunction with embodiment.
Embodiment 1
The first step: the separation of probiotics, purifying and preparation
A. separate: getting chyme in the crucian enteron aisle of health is the bacterium source, utilize MRS lactic acid bacteria culture medium to carry out strain separating, be separated to two kinds of lactic acid bacterias, the lactic acid bacteria that this product adopts has two kinds, for: Lactobacillus casei and licheniformis (13123), Bifidobacterium (Bifidobacterium ATCC7469).
In the prawn enteron aisle, utilize yeast extract wort agar culture medium to separate maternity leave gastral cavity yeast, (Candidautilis CBCC5638);
B. purifying: adopt the lactic acid bacteria culture medium to rule, separate Lactobacillus casei and the licheniformis, the Bifidobacterium (Bifidobacterium) that are separated to, false gastral cavity Candida (Candida utilis) utilizes yeast extract wort agar culture medium to rule, separate, and the bacterial classification colony characteristics that requirement separates is obvious, colonial morphology is neat;
C. the production production of hybrid seeds: the bacterial classification that will rule, separates is inoculated into respectively in the eggplant bottle that fills nutrient agar, cultivates 28 hours under 32 ℃ of temperature, observes when lawn is yellow-white and is covered with eggplant bottle inclined-plane in the eggplant bottle, puts into 4 ℃ of refrigerators and keeps;
MRS lactic acid bacteria culture medium: yeast extract 7.5g, peptone 7.5g, glucose) 10g, potassium dihydrogen phosphate 2g, the 100ml of Tomato juice, Tween (tween) 80 0.5ml, Disti distilled water) 900ml pH7.0.
Yeast extract wort agar culture medium: malt flour 3g, yeast extract 0.1g, agar 15-20g, distilled water 1000ml.
Nutrient agar: beef extract 3g, peptone 10g, sodium chloride 5g, agar 15-20g, water 1000ml, pH7.0-7.2.
These culture mediums all can be found in the relevant operation in laboratory book.
Second step: the Liquid Culture of bacterial classification
A. take by weighing each bacterial classification of the step 1 production production of hybrid seeds by following weight proportion: Lactobacillus casei 30%, Bifidobacterium 30% and false gastral cavity Candida 40%;
B. with the Lactobacillus casei that takes by weighing, Bifidobacterium, false gastral cavity Candida in 1: 20 ratio then in 121 ℃, the fluid nutrient medium of 30min sterilization, put into fermentation tank and stir 30min, its rotating speed is 200r/min, Plastic Drum left standstill 10 days after putting into the sterilization of 100L after stirring, leave standstill in the process and to measure the Ph value every day, fermentation ends when Ph arrives 3.0-4.0;
Its weight percent proportioning of complex medium is: bean cake powder 2%, molasses 9%, dusty yeast 3%, potassium dihydrogen phosphate 0.2%, sodium dihydrogen phosphate 0.5%, sterilized water 85.3%;
The 3rd step: concentrate absorption with vegetable protein
A. after the fermentation ends, being the screw settling centrifuge of 2700r/min by rotating speed earlier with zymotic fluid, is that the butterfly centrifugal machine of 6800r/min concentrates by rotating speed again, and concentration time is 5 hours;
B. concentrate the back and collect centrifugal thing, add corn protein powder in 1% ratio, the mixture after the interpolation stirs in conical agitator, and control moisture is 50%;
The 4th step: dry and pulverizing
A. adopt the Clothoid type drying machine, Steam Heating is carried out drying, the control temperature is at 40 ℃, time 60min in drying, make the suitable Maillard reaction of dry deposits yields, make product color be milk yellow, smell fragrance is strong, further improves palatability, moisture is detected in the dry back of finishing, require dried material moisture≤12%, color and luster is faint yellow or yellowish-brown;
B. crushing screening: dried material is pulverized on pulverizer, and the material after the pulverizing is crossed 50 eye mesh screens, packs into behind the sieve to have in the bucket or bag of inner bag, tightens sack, fastens Sign Board, changes warehouse for finished product over to and stacks in order.
Embodiment 2
The first step: with embodiment 1;
Second step: the Liquid Culture of bacterial classification
A. take by weighing each bacterial classification of the step 1 production production of hybrid seeds by following weight proportion: Lactobacillus casei 40%, Bifidobacterium 40% and false gastral cavity Candida 20%;
B. with the Lactobacillus casei that takes by weighing, Bifidobacterium, false gastral cavity Candida in 1: 33 ratio then in 121 ℃, the liquid complex medium of 40min sterilization, put into fermentation tank and stir 30min, its rotating speed is 200r/min, Plastic Drum left standstill 13 days after putting into the sterilization of 100L after stirring, leave standstill in the process and to measure the Ph value every day, fermentation ends when Ph arrives 3.0-4.0;
Complex medium weight percent proportioning is: bean cake powder 5%, molasses 15%, dusty yeast 5%, potassium dihydrogen phosphate 0.5%, sodium dihydrogen phosphate 0.8%, magnesium sulfate 0.1%, sterilized water 73.6%.
The 3rd step: concentrate absorption with vegetable protein:
A. after the fermentation ends, being the screw settling centrifuge of 3200r/min by rotating speed earlier with zymotic fluid, is that the butterfly centrifugal machine of 7300r/min concentrates by rotating speed again, and concentration time is 7 hours;
B. concentrate the back and collect centrifugal thing, add corn protein powder in 3% ratio, the mixture after the interpolation stirs in conical agitator, and control moisture is 65%;
The 4th step: dry and pulverizing
A. adopt the Clothoid type drying machine, Steam Heating is carried out drying, the control temperature is at 45 ℃, time 80min in drying, make the suitable Maillard reaction of dry deposits yields, make product color be milk yellow, smell fragrance is strong, further improves palatability, moisture is detected in the dry back of finishing, require dried material moisture≤12%, color and luster is faint yellow or yellowish-brown;
B. with embodiment 1;
Add in fish, the shrimp feed in 0.8-1.0 ‰ ratio during use and stir, this product needs to seal up for safekeeping in that dry ventilation is airtight, should use as early as possible behind the Kaifeng.This product uses the back to culture fish body food ration every day will obviously be increased, daily gain obviously improves, in the popularization experiment that carry out at Spring in Pig House of Shanghai Fengxian aquatic products technical advice station, the per mu yield on test each pool, the pool is 151.8 jin, and the average product on the contrast pool only is 70 jin, the output on the test pool has improved 116.9%, and difference is quite obvious, and feed coefficient has on average reduced by 17.1%.And the individuality of respectively testing pool freshwater shrimp is bigger, body surface smooth, and body colour is blue or green, and selling price is higher, and each pool freshwater shrimp body surface of the contrast pool is crude, drift is more, and body colour is whiter, and selling price is not high.
Claims (3)
1. the fish and shrimp phagostimulant that the tunning adhesion protein is produced is characterized in that, and is formulated by following percentage by weight: Lactobacillus casei 30-40%, Bifidobacterium 30-40%, false gastral cavity Candida 20-40%.
2. the preparation method of the fish and shrimp phagostimulant of producing according to the described a kind of tunning adhesion protein of right 1, it is characterized in that, adopt fluid enlargement culture, in concentration process, adopt cryogenic separation and dry technology, add vegetable protein simultaneously in concentrating, its preparation process is:
The first step: the separation of probiotics, purifying and preparation
A. separate: getting chyme in the crucian enteron aisle of health is the bacterium source, utilize the lactic acid bacteria culture medium to carry out strain separating, be separated to two kinds of lactic acid bacterias, the lactic acid bacteria that this product adopts has two kinds, for: Lactobacillus casei and, Bifidobacterium utilizes yeast extract wort agar culture medium to separate a kind of saccharomycete in the prawn enteron aisle, and the saccharomycete that the present invention adopts is a maternity leave gastral cavity Candida;
B. purifying: the Lactobacillus casei that separation is obtained adopts the lactic acid bacteria culture medium to rule, separate with Bifidobacterium, and false gastral cavity Candida utilizes yeast extract wort agar culture medium to rule, separate;
C. the production production of hybrid seeds: the bacterial classification inoculation that will rule, separates was cultivated 28-30 hour under 30-35 ℃ of temperature to the eggplant bottle that fills nutrient agar, observed when lawn is yellow-white and is covered with eggplant bottle inclined-plane in the eggplant bottle, put into 4 ℃ of refrigerators and kept;
Second step: the Liquid Culture of bacterial classification
A. take by weighing Lactobacillus casei, Bifidobacterium and the false gastral cavity Candida of the step 1 production production of hybrid seeds by weight ratio, inoculating proportion of the present invention is: Lactobacillus casei 30-40%, Bifidobacterium 30-40%, false gastral cavity Candida 20-40%;
B. with the Lactobacillus casei that takes by weighing, Bifidobacterium, false gastral cavity Candida in 1: the ratio of 20-33 is then in 120-130 ℃, the complex medium of 30-40min sterilization, put into fermentation tank then and stir 30min, its rotating speed is 200r/min, putting into sterilization back Plastic Drum after stirring left standstill 10-13 days, leave standstill in the process and to measure the Ph value every day, fermentation ends when Ph arrives 3.0-4.0;
The 3rd step: concentrate absorption with vegetable protein
A. after the fermentation ends, being the screw settling centrifuge of 2700-3200r/min by rotating speed earlier with zymotic fluid, is that the butterfly centrifugal machine of 6800-7300r/min concentrates by rotating speed again, and concentration time is 4-8 hour;
B. concentrate the back and collect centrifugal thing, add corn protein powder in the 1-3% ratio, the mixture after the interpolation stirs in conical agitator, and control moisture is at 50-65%;
The 4th step: dry and pulverizing
A. adopt the Clothoid type drying machine, Steam Heating is carried out drying, the control temperature is at 40-45 ℃, time 60-80min in drying, moisture is detected in the dry back of finishing, require dried material moisture≤12%, color and luster is faint yellow or yellowish-brown, makes dry deposits yields Maillard reaction, and smell fragrance is strong;
B. crushing screening: dried material is pulverized on pulverizer, and the material after the pulverizing is crossed 50 eye mesh screens, packs into behind the sieve to have in the bucket or bag of inner bag, tightens sack, fastens Sign Board, changes warehouse for finished product over to and stacks in order.
3. the preparation method of the fish and shrimp phagostimulant that a kind of tunning adhesion protein according to claim 2 is produced, it is characterized in that, described complex medium is at least three kinds of mixtures that sterilized water adds bean cake powder, urea, clear chaff, ammonium sulfate, dusty yeast, corn flour, molasses, potassium dihydrogen phosphate, sodium hydrogen phosphate, magnesium phosphate, magnesium sulfate again, and its weight percent proportioning is: bean cake powder 2-5%, molasses 9-15%, dusty yeast 2-5%, potassium dihydrogen phosphate 0-0.6%, sodium dihydrogen phosphate 0.5-0.8%, magnesium sulfate 0-0.1%, sterilized water 73.6-85.5%.
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