CN101084759A - Method for using pickle lactobacillus fermentation to impart antibiotic activity on object surface - Google Patents
Method for using pickle lactobacillus fermentation to impart antibiotic activity on object surface Download PDFInfo
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- CN101084759A CN101084759A CN 200610014185 CN200610014185A CN101084759A CN 101084759 A CN101084759 A CN 101084759A CN 200610014185 CN200610014185 CN 200610014185 CN 200610014185 A CN200610014185 A CN 200610014185A CN 101084759 A CN101084759 A CN 101084759A
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- pickle
- lactobacillus fermentation
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- 241000186660 Lactobacillus Species 0.000 title claims abstract description 46
- 229940039696 lactobacillus Drugs 0.000 title claims abstract description 46
- 238000000034 method Methods 0.000 title claims abstract description 24
- 235000021110 pickles Nutrition 0.000 title claims description 60
- 238000000855 fermentation Methods 0.000 title claims description 41
- 230000004151 fermentation Effects 0.000 title claims description 41
- 230000003115 biocidal effect Effects 0.000 title claims description 16
- 239000011248 coating agent Substances 0.000 claims abstract description 17
- 238000000576 coating method Methods 0.000 claims abstract description 17
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims description 36
- 241000894006 Bacteria Species 0.000 claims description 25
- 239000004310 lactic acid Substances 0.000 claims description 18
- 235000014655 lactic acid Nutrition 0.000 claims description 18
- 239000011230 binding agent Substances 0.000 claims description 15
- 239000011259 mixed solution Substances 0.000 claims description 14
- 239000012531 culture fluid Substances 0.000 claims description 10
- 238000005406 washing Methods 0.000 claims description 10
- 239000004925 Acrylic resin Substances 0.000 claims description 7
- 229920000178 Acrylic resin Polymers 0.000 claims description 7
- 239000000203 mixture Substances 0.000 claims description 7
- 229920001296 polysiloxane Polymers 0.000 claims description 7
- 238000004851 dishwashing Methods 0.000 claims description 6
- 241000192132 Leuconostoc Species 0.000 claims description 3
- 239000012141 concentrate Substances 0.000 claims description 3
- 229920005749 polyurethane resin Polymers 0.000 claims description 3
- 241000588807 Bordetella Species 0.000 claims description 2
- 230000004048 modification Effects 0.000 claims description 2
- 238000012986 modification Methods 0.000 claims description 2
- 230000000845 anti-microbial effect Effects 0.000 abstract description 6
- 244000005700 microbiome Species 0.000 abstract description 3
- 238000001228 spectrum Methods 0.000 abstract description 3
- 238000004140 cleaning Methods 0.000 description 12
- 239000000428 dust Substances 0.000 description 10
- 238000012360 testing method Methods 0.000 description 10
- 229940037003 alum Drugs 0.000 description 9
- 239000000463 material Substances 0.000 description 8
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 6
- 230000001954 sterilising effect Effects 0.000 description 6
- 241000589517 Pseudomonas aeruginosa Species 0.000 description 5
- 241000191967 Staphylococcus aureus Species 0.000 description 5
- 241000700605 Viruses Species 0.000 description 5
- 230000000844 anti-bacterial effect Effects 0.000 description 5
- 230000000840 anti-viral effect Effects 0.000 description 5
- 238000004519 manufacturing process Methods 0.000 description 5
- 241000193830 Bacillus <bacterium> Species 0.000 description 4
- 210000001072 colon Anatomy 0.000 description 4
- 229910052751 metal Inorganic materials 0.000 description 4
- 239000002184 metal Substances 0.000 description 4
- 238000012797 qualification Methods 0.000 description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- 230000001580 bacterial effect Effects 0.000 description 3
- 239000000835 fiber Substances 0.000 description 3
- 230000036541 health Effects 0.000 description 3
- 238000011534 incubation Methods 0.000 description 3
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- 239000003242 anti bacterial agent Substances 0.000 description 2
- 206010064097 avian influenza Diseases 0.000 description 2
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- 238000010438 heat treatment Methods 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 239000007921 spray Substances 0.000 description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 229920003043 Cellulose fiber Polymers 0.000 description 1
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 1
- 229920000742 Cotton Polymers 0.000 description 1
- 241001360526 Escherichia coli ATCC 25922 Species 0.000 description 1
- 241000202221 Weissella Species 0.000 description 1
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- NIXOWILDQLNWCW-UHFFFAOYSA-N acrylic acid group Chemical group C(C=C)(=O)O NIXOWILDQLNWCW-UHFFFAOYSA-N 0.000 description 1
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- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
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- 238000002156 mixing Methods 0.000 description 1
- 239000002504 physiological saline solution Substances 0.000 description 1
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- 244000144977 poultry Species 0.000 description 1
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- 230000001105 regulatory effect Effects 0.000 description 1
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Apparatus For Disinfection Or Sterilisation (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention relates to a method for enduing objective surface with micro-organism resistance with kraut lactobacillus leavening, which is characterized in coating the mixed liquor of kraut lactobacillus leavening and agglomerant on objective surface. The invention makes use of kraut lactobacillus leavening with broad antimicrobial spectrum, thereby exerting the micro-organism resistance of household appliance.
Description
Background technology
The present invention relates to utilize the method for pickle lactobacillus fermentation to impart antibiotic activity on object surface, say so more specifically, will have the method for mixed solution coating on the various object surfaces that bacterium or virus etc. may contact, breed of the pickle lactobacillus fermentation and the binding agent of antibiotic and antiviral effect.
Recently, along with the concern to surrounding environment increases year by year, the user need give antimicrbial power in the life household appliances directly related with the human body health, thereby can satisfy user's health demand and close environmental demand simultaneously.Wherein, need to keep the household appliances of health, cleaning to have the refrigerator of comprising, washing machine, air-conditioner, dust catcher, warm air drier, electric food warmer, electric cooker, dish-washing machine, micro-wave oven, VTR, calculator, TV etc. multiple.
For endowing antibacterial energy in above-mentioned household appliances, generally use organic and inorganic antibacterial agent etc.But, using under the situation of organic antibacterial agent, will be difficult to continue its result of use, and to the stability action of human body also a little less than.
Pickle lactobacillus fermentation is the product that draws by in the fermentation process as the pickles of Korean traditional diet, above-mentioned pickle lactobacillus fermentation is by absorbing for a long time in the human body, thereby can guarantee its stability, and have easy picked-up and a comparatively economic advantage, be that a kind of antimicrbial power is higher, and have the natural antibacterial material of wider antimicrobial spectrum.
And, the result draws in the light of recent researches, pickle lactobacillus fermentation has good effect to the prevention and the treatment of the viral disease of poultries such as bird flu, and the present inventor has proposed the present invention based on the antibiotic and antiviral effect of above-mentioned pickle lactobacillus fermentation.
Summary of the invention
The object of the present invention is to provide a kind of method of mixed solution coating on the various object surfaces that bacterium or virus etc. may contact, breed that will have the pickle lactobacillus fermentation and the binding agent of antibiotic and antiviral effect.
To achieve these goals, the present invention is characterized in that: with the mixed solution coating of pickle lactobacillus fermentation and binding agent on object surfaces.
Above-mentioned pickle lactobacillus fermentation can be selected from the group who is made of the culture fluid of pickle lactic acid bacteria, the concentrate of culture fluid, dry thing of culture fluid and composition thereof.Above-mentioned pickle lactic acid bacteria can be selected from the group who is made of Leuconostoc (Leuconostoc) pickle lactic acid bacteria, lactobacillus (Lactobacillus) pickle lactic acid bacteria, Wei Si Bordetella (Weissella) pickle lactic acid bacteria and composition thereof.
Select the group that above-mentioned binding agent can constitute from acrylic resin, polyurethane resin, acrylic resin and the silicones by the silicones modification.
Various objects, particularly life household appliances that above-mentioned object can may contact, breed for bacterium or virus etc., for example refrigerator, washing machine, air-conditioner, air purifier, dust catcher, dish-washing machine, micro-wave oven, or the internal structure external structure parts of the said goods.And the present invention is characterized in that: above-mentioned object is a filter.
And, in above-mentioned coating step, the mixed solution of pickle lactobacillus fermentation and binding agent is sprayed at body surface, or object is immersed in the above-mentioned mixed solution.
The method of utilizing pickle lactobacillus fermentation to impart antibiotic activity on object surface in according to the present invention has been utilized the pickle lactobacillus fermentation with wider antimicrobial spectrum among the present invention, thereby can give full play to the anti-microbial property of household appliances object.And the antiviral performance by pickle lactic acid bacteria has can reach stronger safely and effectively antiviral effect to the virus that causes bird flu.
Embodiment
Below, with the present invention will be described in detail.
Pickle lactobacillus fermentation among the present invention obtains in the maturation process of pickles.The pickle lactic acid bacteria that uses among the present invention can maybe can be bought the pickle lactic acid bacteria of selling on the market it is cultivated use by directly extracting use from pickles, and, can use the lactic acid bacteria of any state, and be not to have special qualification.And the culture fluid of above-mentioned pickle lactic acid bacteria can be that to select from the group who is made of the culture fluid of pickle lactic acid bacteria, the concentrate of culture fluid, dry thing of culture fluid and composition thereof is a certain.
Can use silicones acrylic resin modified (siliconemodified acrylic resin), polyurethane resin, acrylic resin and silicones etc. as the binding agent among the present invention, but also not be to be defined in this, can use multiple binding agent.Under the situation of using binding agent, can be easy to be solidificated in pickle lactobacillus fermentation on the body surface, and improve the active force that mutually combines of body surface and pickle lactobacillus fermentation, thus the flood rate of pickle lactobacillus fermentation can be reduced, and keep good antibacterial performance.
Various objects, particularly life household appliances that object among the present invention can may contact, breed for bacterium or virus etc., for example, refrigerator, washing machine, dust catcher, air purifier, air-conditioner, dish-washing machine, various device for cooking etc.And above-mentioned object can be each structure member of the inside and outside that constitutes various household appliances.Wherein, the object raw material is not particularly limited, so long as can constitute the inside and outside of household appliances, can use any material, for example, can use materials such as thermoplastic resin or thermosetting resin, rubber, metal, above-mentioned material can use according to different purposes according to the characteristic of its material.
And the object among the present invention can be air cleaning filter, at this moment, so long as have the function of air cleaning filter, will not have special the qualification as the object raw material on its kind, shape, size and the manufacture method.For example, can use the glass fibre of glass fibre, ion-exchange fibre, cellulose fiber, asbestos fibre etc. and various organic fiber, inorfil, perhaps can use the metal or the plastics of zinc, copper, aluminium etc., above-mentioned material can be divided into different purposes according to its material characteristic to be used.And it can use honeycomb type, corpuscular type, net type, filter paper type, cotton type, aperture plate type, sheet type and template etc. in shape, and there is no particular restriction, can be according to suitably distortion and use of the air cleaning unit of using.Above-mentioned filter can be applicable to deodorization filters such as active carbon filter, the high-performance filter (HEPA filter) that uses in the multiple household appliances such as refrigerator or air-conditioner, and use, or use in the lump with above-mentioned each filter as the filters such as air cleaning unit of automobile.And the object among the present invention is except air cleaning filter, also applicable in the water-purifying filter.
In with the process of pickle lactobacillus fermentation coating on object, pickle lactobacillus fermentation is mixed with binding agent, then above-mentioned mixed solution is injected in object surfaces by spray regime, or object is immersed in the container that fills above-mentioned mixed solution.So long as pickle lactobacillus fermentation can mix with binding agent in the degree of body surface with coating, will there be special the qualification on the manufacture method of above-mentioned mixed solution.The concentration of above-mentioned pickle lactobacillus fermentation is 5～20 weight % preferably, but are not to be defined in this, can as required and suitably regulate and use.And, there is no special qualification on the content ratio of pickle lactobacillus fermentation and binding agent, can as required and suitably regulate and use.
In addition, can add following steps as the step before the coating: with the suitable clean target object of clean washing, and by above-mentioned clean target object of oven dry such as heat treatment operations.And, can add following steps as the step after the coating: dry by heat treatment operation etc.Under the situation of oven dry object, its drying time and baking temperature can be regulated according to the shape of the object that uses, kind, size etc.And according to circumstances,, be preferably in and remove its surface in its manufacture process or the keeping and go up oil formulation of pasting for the object of metal etc.
Below, will the embodiment among the present invention be described in detail, but the present invention is defined in embodiment as described below.
Embodiment 1
To be immersed in from the alum gate net that air cleaner company buys the 2.5%NaOH about 3 minutes, and the remover oil component, then, the alum gate net of above-mentioned remover oil component to be cleaned with 2.5%NaOH, aforesaid operations carries out 7 times repeatedly.Then, the above-mentioned alum gate net that obtains cleaning is placed in the oven dry baking box that is adjusted to 40 ℃ of temperature, carries out thermal treatment in 2 hours.Simultaneously, with pickle lactobacillus fermentation and silicones modified propylene acid binder is the ratio mixing of 5～20 weight % with pickle lactobacillus fermentation, and making mixed solution, then above-mentioned mixed solution is sprayed and carry out coating on the net at above-mentioned heat treated alum gate, consequently, with obtaining coating the alum gate net of pickle lactic acid bacteria is arranged.
Experimental example 2
In order to detect the coating of making as mentioned above the anti-microbial property of the air cleaning filter of pickle lactobacillus fermentation to colon bacillus, staphylococcus aureus and Pseudomonas aeruginosa arranged, its sterilizing power is tested according to the method for KS M 0146-2003.
Promptly, utilize experimental rigs such as high pressure sterilization device, bacterial culture thermostat, sterile board, colonometer (ColonyCounter), Water Tank with Temp.-controlled, extinction photometer, the bacterial strain of colon bacillus (Escherichia coliATCC 25922), staphylococcus aureus (Staphylococcus aureus ATCC 6538) and Pseudomonas aeruginosa (Pseudomonas aeruginosa ATCC 27853) has been carried out the sterilizing power test respectively.Above-mentioned bacterial strains is coated in (each 1.0ml) on the test test portion of the alum gate web filter 1.0 * 1.0cm size among the present invention who obtains by Production Example, simultaneously, 3 contrast test portions with an inoculation test bacterium liquid, 3 test test portions are positioned in the conical flask (Erlenmeyer flask), and cultivate 1 hour, 2 hours, 3 hours under 35 ± 1 ℃, the condition of 120RPM.Then, extract above-mentioned bacterium liquid, and utilize physiological saline to dilute, and under 37 ℃ temperature condition, in dull and stereotyped incubator, cultivated 48 hours, confirm bacterium quantity, its result with reference to as following table 1 to shown in the table 3.
Table 1: to the sterilizing power of colon bacillus
| Incubation time | ||||
| 0 | After 1 hour | After 2 hours | After 3 hours | |
| The contrast test portion | 1.5×10 5CFU/ml | 1.6×10 5CFU/ml | 1.7×10 5CFU/ml | 2.0×10 5CFU/ml |
| The alum gate web filter | 1.5×10 5CFU/ml | ＜10CFU/ml | ＜10CFU/ml | ＜10CFU/ml |
| 99.9 | 99.9 | 99.9 | ||
Table 2: to the sterilizing power of staphylococcus aureus
| Incubation time | ||||
| 0 | After 1 hour | After 2 hours | After 3 hours | |
| The contrast test portion | 1.3×10 5CFU/ml | 1.5×10 5CFU/ml | 1.8×10 5CFU/ml | 2.2×10 5CFU/ml |
| The alum gate web filter | 1.3×10 5CFU/ml | ＜10CFU/ml | ＜10CFU/ml | ＜10CFU/ml |
| 99.9 | 99.9 | 99.9 | ||
Table 3: to the sterilizing power of Pseudomonas aeruginosa
| Incubation time | ||||
| 0 | After 1 hour | After 2 hours | After 3 hours | |
| The contrast test portion | 1.2×10 5CFU/ml | 1.4×10 5CFU/ml | 1.7×10 5CFU/ml | 2.2×10 5CFU/ml |
| The alum gate web filter | 1.2×10 5CFU/ml | ＜10CFU/ml | ＜10CFU/ml | ＜10CFU/ml |
| 99.9 | 99.9 | 99.9 | ||
According to aforesaid table 1 to table 3 as can be known, the air cleaning filter that comprises pickle lactic acid bacteria among the present invention will significantly improve the bactericidal effect to microorganisms such as colon bacillus, staphylococcus aureus, Pseudomonas aeruginosas.
Be suitable for example
Method among the present invention is applicable to various uses as described below, object by the method manufacturing among the present invention is the multiple household appliances that need possess anti-microbial property, can be refrigerator, washing machine, air-conditioner, dust catcher, air purifier, water purifier, humidifier, dish-washing machine, micro-wave oven, VTR, calculator, TV etc.
1) application in refrigerator
Can be applicable to inwall, cool-air duct, blowing fan, door handle of refrigerator, vegetables box, air cleaning filter, ice maker, the carriage of refrigerator, surface of refrigerator outside etc.
2) application in washing machine
But coating is on the surface of washing machine structure parts such as inside groove, water jacket, vibratory sieve, washing agent tube, cylinder lifting device, washing tub, and, also can be applicable to the filter (air cleaning filter, water-purifying filter) in dry stream, plumbing stream and the circulation stream.
3) application in air-conditioner
Can be applicable to cooling pin (pin), electric heating switch (ventilation element), blowing fan (metal, injected material), air flow circuit (suction portion, discharge portion, shutter, grid etc.) in the heat exchanger, and the air cleaning filter of indoor set.
4) effect in air purifier
The pickle lactobacillus fermentation filter can be installed in the multi-filter of air purifier in addition.
5) application in dust catcher
Under the situation of dust catcher, situation about touching owing to directly catching is more, therefore can carry out coating to the surface of dust catcher.And, also can carry out coating and handle, thereby in the dust tube, obtain further bactericidal effect the inside of dust tube, in addition, also can be applicable in the discharge filter of dust catcher.
6) application in dish-washing machine
Can be applicable to wash the dishes tube, water leg, adopt in dirty net, spray arm, the water-purifying filter etc.
More than be illustrated at the preferred embodiment among the present invention, but the present invention is defined in aforesaid embodiment, in the category that does not exceed the technology of the present invention thought, the operator of relevant industries can carry out various deformation and application to it.
Claims (9)
1, a kind of method of utilizing pickle lactobacillus fermentation to impart antibiotic activity on object surface is characterized in that:
With the mixed solution coating of pickle lactobacillus fermentation and binding agent on object surfaces.
2, the method for utilizing pickle lactobacillus fermentation to impart antibiotic activity on object surface according to claim 1 is characterized in that:
Above-mentioned object is household appliances such as refrigerator, washing machine, air-conditioner, air purifier, dish-washing machine.
3, the method for utilizing pickle lactobacillus fermentation to impart antibiotic activity on object surface according to claim 2 is characterized in that:
Above-mentioned object is the internal structure external structure parts of household appliances.
4, the method for utilizing pickle lactobacillus fermentation to impart antibiotic activity on object surface according to claim 1 is characterized in that:
Above-mentioned object is a filter.
5, the method for utilizing pickle lactobacillus fermentation to impart antibiotic activity on object surface according to claim 1 is characterized in that:
Above-mentioned binding agent is selected from the group that acrylic resin, polyurethane resin, acrylic resin and silicones by the silicones modification constitute.
6, the method for utilizing pickle lactobacillus fermentation to impart antibiotic activity on object surface according to claim 1 is characterized in that:
Above-mentioned pickle lactobacillus fermentation is selected from the group who is made of the culture fluid of pickle lactic acid bacteria, the concentrate of culture fluid, dry thing of culture fluid and composition thereof.
7, according to claim 1 or the 6 described methods of utilizing pickle lactobacillus fermentation to impart antibiotic activity on object surface, it is characterized in that:
Above-mentioned pickle lactic acid bacteria is selected from the group who is made of Leuconostoc pickle lactic acid bacteria, lactobacillus pickle lactic acid bacteria, Wei Si Bordetella pickle lactic acid bacteria and composition thereof.
8, the method for utilizing pickle lactobacillus fermentation to impart antibiotic activity on object surface according to claim 1 is characterized in that:
In above-mentioned coating step, the mixed solution of pickle lactobacillus fermentation and binding agent is sprayed at body surface, or object is immersed in the above-mentioned mixed solution.
9, according to claim 1 or the 8 described methods of utilizing pickle lactobacillus fermentation to impart antibiotic activity on object surface, it is characterized in that:
Pickle lactobacillus fermentation contains the amount of 5～20 weight % in mixed solution.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200610014185 CN101084759A (en) | 2006-06-08 | 2006-06-08 | Method for using pickle lactobacillus fermentation to impart antibiotic activity on object surface |
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| Application Number | Priority Date | Filing Date | Title |
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| CN 200610014185 CN101084759A (en) | 2006-06-08 | 2006-06-08 | Method for using pickle lactobacillus fermentation to impart antibiotic activity on object surface |
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| CN101084759A true CN101084759A (en) | 2007-12-12 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103834211A (en) * | 2013-12-27 | 2014-06-04 | 青岛碧水蓝天生物技术有限公司 | Method of endowing object surface with antibacterial property by using lactobacillus ferment |
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- 2006-06-08 CN CN 200610014185 patent/CN101084759A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103834211A (en) * | 2013-12-27 | 2014-06-04 | 青岛碧水蓝天生物技术有限公司 | Method of endowing object surface with antibacterial property by using lactobacillus ferment |
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