CN101070531A - Dry-powder-type culturing substrate and preparing method - Google Patents

Abstract

Powder type culture medium is from plenty of element kinds , trace element and iron salt and vitamin kind of composition, after processing manufacture the original powder that makes powder culture medium. The various components of the medium and the final product were dry powder. The preparation Method: First, concentrate all plenty of elements to collect reserve; Mix trace element and vitamin kind totally to wrap to attach , and then mix the thin medicine and plenty of elements of trace element totally, under the role with centrifugal high speed, making it even scatter and wrap up to glue to unite. So, make the original powder of powder culture medium for manufacture. This medium-dry powder manufacturing easier, does not require the use of complex machines and equipment, and low-cost easy to promote. The product is of high quality in existing product. It can be used in plant breeding, tissue culture and molecular biology research, and can be used for various commercial purposes.

Description

Dry-powder-type culturing substrate and preparation method thereof

Technical field

The present invention designs biological technical field, is specifically related to preparation method and using method that plant clone technique is applied to the substratum of scientific research and extensive plant tissue culture production usefulness.This product and method are of value to plant breeding, tissue culture and molecular biology research person, and can be used for various commercial purposes.

Background technology

Normal growth and growth for the tissue culture plant need substratum.The main component of existing plant cloning substratum includes these several big class materials of macroelement class (as saltpetre, ammonium nitrate, calcium chloride etc.), trace element, molysite class (ferrous sulfate, copper sulfate, manganous sulfate etc.), vitamins (VB1, VB6 etc.), peptizer (agar, condensation fat or the safe glue that shakes) and sucrose and forms.

Traditional configuration way is peptizer to be added obtain substratum after regulating potential of hydrogen behind the water-soluble mother liquor that adds other several materials behind the water boil.And the mother liquor of preparation macroelement class, trace element and vitamins, molysite class, step is very loaded down with trivial details.

The preparation process of standard is: have to again prepare the respectively mother liquor (jointly mix to permit in 1000 times mother liquor precipitation or the like can occur as saltpetre and calcium chloride) of many kinds of the chemical reaction between the various elements of considering each big class under the situation of high density (1000 times of concentration), (ratio is generally following 1000 times of normal temperature situation) adding each mother liquor respectively in the substratum of boiling that will use according to 1: 1 ratio in order so each element is dissolved in the water.Do not only inefficiency like this, and the difficult preservation of mother liquor, be very easy to cause mother liquor to pollute.The entire operation link is human intervention fully, is very easy to cause the batching mistake, has influence on the accuracy of configuration substratum.

Summary of the invention

Bothersome, loaded down with trivial details basic disadvantage when solving existing configuration substratum, the present invention mainly is to provide a kind of dry-powder-type culturing substrate and preparation method thereof.

Dry-powder-type culturing substrate is made up of macroelement class, trace element and molysite and vitamins, goes out the former powder of dehydrated medium through processing and manufacturing; At different service requirementss, can when adding macroelement, add sucrose again, produce the full powder of substratum.The principal character of substratum is that also each constituent and last finished product all are dry powder.

The preparation method is: at first that all macroelement centralized collection is standby; Microelement kind and vitamins blend bag is attached, again with thin medicine of trace element and macroelement blend, under ultracentrifugal effect, make the sticking connection of its homodisperse and parcel.So just produce the former powder of dehydrated medium.

During use,, in the preparation substratum, only need obtain dry-powder-type culturing substrate as required and directly add (its matched proportion density is 1: 1, so just can not occur having precipitated) in the entry, promptly obtain the liquid substratum of standard just as soluble coffee.

This dry-powder-type culturing substrate raw material is simple and easy to, and is easy to manufacture, do not need to use complicated machinery equipment, and with low cost being easy to promoted.The quality of product is higher than currently available products.Can be widely used in plant breeding, tissue culture and molecular biology research, and can be used for various commercial purposes.

Embodiment

In order to realize dry-powder-type culturing substrate of the present invention, the invention provides following technical scheme:

Substratum with preparation standard illustrates now, and following table is the prescription and the classification of the substratum of one liter of amount of preparation:

The macroelement class:	Saltpetre	1.9g
			Ammonium nitrate	1.65g
	Calcium dichloride dihydrate	0.44g
			Magnesium sulfate heptahydrate	0.37g
	Potassium primary phosphate	0.17g
			Trace element and molysite:	Disodium ethylene diamine tetraacetate	37.3mg
Iron vitriol	27.8mg
		Four water manganous sulfates		22.3mg
Zinc Sulphate Heptahydrate	8.6mg
		Boric acid		6.2mg
Potassiumiodide	0.83mg
		Sodium Molybdate Dihydrate		0.25mg
Cupric sulfate pentahydrate	0.025mg
		CoCL2		0.025mg
Vitamins:	VB1				0.1mg
		VB6	0.5mg
	Nicotinic acid			0.5mg
		Inositol	0.1g
	Glycine			2mg
		Sucrose:	Sucrose		30g

Concrete preparation method is as follows:

1, determined the prescription and the quantity of dry-powder-type culturing substrate earlier, now illustrated with the substratum for preparing 1000 raising of indices standards;

2, all macroelement centralized collection are standby;

3, with microelement kind and vitamins blend, make micro mist through molecule bag random again and handle, make it divide Bales Off attached fully, guarantee its uniformity coefficient and good dispersiveness, produce the thin medicine of trace element;

4, thin medicine of trace element and macroelement are added the blend of high speed blender, under the ultracentrifugal effect of high speed blender, utilize the big characteristic of macroelement molecular mass to make its homodisperse and the sticking connection of parcel like this.So just produce the former powder of dehydrated medium;

5, at different service requirementss, can when adding macroelement, add sucrose again, produce the full powder of substratum.

Using method is divided into:

No matter full powder or former powder when 1, using, existing it is dissolved in the cold water of the substratum that disposes 1/10th obtains solution.

2, the using method of the former powder of dry-powder-type culturing substrate of standard: peptizer added add its dissolving of thermic in the entry, the back adds sucrose, adds the former powder solution of substratum again, regulates to be filled to after the potential of hydrogen just to be prepared into standard medium in the culture vessel.Can reach different culture effect according to the usage ratio that purposes is regulated sucrose and peptizer like this.

3, the hot filling using method of full powder: peptizer adds in the entry and melts to it with sucrose after the heating, adds the former powder solution of substratum again, is filled in the culture vessel after regulating potential of hydrogen.

4, the cold filling using method of full powder: peptizer adds in the entry, adds three-dimensional stirring of the full powder solution of substratum again and makes the active substance homodisperse, directly is filled in the culture vessel after the adjusting potential of hydrogen.

Claims (6)

1, a kind of dry-powder-type culturing substrate is made up of macroelement class, trace element and molysite and vitamins, it is characterized in that: the macroelement class includes saltpetre, ammonium nitrate, Calcium dichloride dihydrate, magnesium sulfate heptahydrate, potassium primary phosphate; Trace element and molysite include disodium ethylene diamine tetraacetate, iron vitriol, four water manganous sulfates, Zinc Sulphate Heptahydrate, boric acid, potassiumiodide, Sodium Molybdate Dihydrate, cupric sulfate pentahydrate, CoCL2; Vitamins includes VB1, VB6, nicotinic acid, inositol, glycine;

Above-mentioned constituent and substratum finished product all are dry powder.

2, dry-powder-type culturing substrate according to claim 1 is characterized in that: can also include sucrose in the substratum.

3, dry-powder-type culturing substrate according to claim 1 and 2 is characterized in that: in the substratum of one liter of amount, the weight of contained each material is:

(1) in the macroelement class: saltpetre 1.9g, ammonium nitrate 1.65g, Calcium dichloride dihydrate 0.44g, magnesium sulfate heptahydrate 0.37g, potassium primary phosphate 0.17g:

(2) in trace element and the molysite: disodium ethylene diamine tetraacetate 37.3mg, iron vitriol 27.8mg, four water manganous sulfate 22.3mg, Zinc Sulphate Heptahydrate 8.6mg, boric acid 6.2mg, potassiumiodide 0.83mg, Sodium Molybdate Dihydrate 0.25mg, cupric sulfate pentahydrate 0.025mg, CoCL2 0.025mg;

(3) vitamins: VB10.1mg, VB60.5mg, nicotinic acid 0.5mg, inositol 0.1g, glycine 2mg;

(4) sucrose: 30g.

4, a kind of method for preparing the dry-powder-type culturing substrate of claim 1 is characterized in that: earlier that described all macroelement centralized collection is standby; With described microelement kind, molysite and vitamins blend, handle through micro mist more then, guarantee uniformity coefficient and good dispersiveness, produce the thin medicine of trace element; Again thin medicine of trace element and macroelement are added the blend of high speed blender, under the ultracentrifugal effect of high speed blender, utilize the big characteristic of macroelement molecular mass to make its homodisperse and the sticking connection of parcel, produce the former powder of dehydrated medium.

5, dry-powder-type culturing substrate preparation method according to claim 4 is characterized in that: described micro mist is handled and is to use molecule bag random to deal with.

6, dry-powder-type culturing substrate preparation method according to claim 4 is characterized in that: can add sucrose when adding macroelement, produce the full powder of substratum.