CN101057609B - Long-acting sour milk containing beneficial bacteria factor and its producing method - Google Patents
Long-acting sour milk containing beneficial bacteria factor and its producing method Download PDFInfo
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Abstract
The invention discloses a long-effect yoghourt and process for preparation, wherein the yoghourt is prepared from the following raw materials (by weight portions): milk 50-100, isomaltose hypgather 0. 01-20. 00 and stabilizing agent 0. 2-8. 0. The nourishing constituents in the yoghourt can be preserved, and the storage period of the product can reach longer than six months.
Description
Technical field
The present invention relates to a kind of long-acting sour milk and production method thereof that contains beneficial bacteria factor.
Background technology
Yoghurt in the market is to be raw material with sweet milk or dairy products, adds sugar, strain fermentation forms.Protein content is not less than the yoghurt that is called of 2.3% (m/V) in the finished product.Long-acting sour milk promptly after heat treatment passes through the fermented yoghourt of aseptic packaging again, but its maximum characteristics be under the normal temperature state holding time be not less than 60 days, and mouthfeel and taste and conventional sour milk are basic identical.The main component of long-acting yoghurt is milk, water, white granulated sugar, thickener, emulsifying agent, acidity regulator and flavorant, according to the requirement of people, in product, add vitamin, mineral matter, amino acid, fruit grain, fruit juice, jam or one or more nutriments of nut granule to health.World dairy products federation had formulated standard to long-acting sour milk in 2003, so it is acknowledged as a kind of certified milk goods of standard.Its trade name remains " sour milk yogurt ", but will be titled with " heat treatment thermized " or other prefix.Nutritive value about long-acting sour milk, the existing final conclusion of world dairy products joint committee: the nutrition of sour milk is not only from viable bacteria, the metabolite that is produced in the Yoghourt fermentation process also has high nutritive value, exists even without viable bacteria, and sour milk remains senior nutritional health food.Concerning the huge lactose incompatibility crowd in Asia, sour milk particularly long-acting sour milk is significant.Therefore, such dairy products had obtained extensive concern in recent years, long-acting sour milk has not only kept the nutritional labeling of sour milk, the shelf-life of product was reached more than six months, enlarged the sales range of product, thereby made the people that are in the mountain village that the geographical position is remote, communications and transportation is inconvenient can enjoy multiple tastes, be of high nutritive value and drink yoghurt easily.
Bifidobacterium is a typical beneficial bacteria in the human body intestinal canal, and its growth and breeding is applied in people's the whole life course.Bifidobacterium growth and breeding under anaerobic environment produces a large amount of lactic acid, reduces the pH of system value and gut flora is changed, and suppresses and kills enteric pathogenic bacteria, makes flora maintenance normal equilibrium.Yet the quantity of Bifidobacterium is still a technical barrier up to now in the raising human body.This is because Bifidobacterium is the anaerobic type flora, in air, be difficult to survival, the acidity of adding hydrochloric acid in gastric juice is very strong, the overwhelming majority all has been killed during Bifidobacterium process stomach, have only few part to arrive in the intestines, thereby make its beneficial functional be difficult to embody, and the flora of the Bifidobacterium of different people is also incomplete same human body, promptly allow to replenish the Bifidobacterium that lives, can not adapt to all crowds.
Oligoisomaltose (IMO) is a kind of of starch sugar, and main component is by α-1, the functional oligose that the isomaltose (IG2) of 6-glycosidic bond combination, panose (P), Isomaltotriose (IG3) and tetrose above (GN) are combined into.Can be divided into two kinds in oligoisomaltose slurry and oligoisomaltose powder by form.Syrup is colourless or light yellow, transparent thick liquid, and sweet taste is soft, free from extraneous odour, the visible impurity of no twenty-twenty vision.Icing Sugar is white amorphous powder, and sweet taste is soft, free from extraneous odour, the visible impurity of no twenty-twenty vision.The basic composition of different purity oligoisomaltose (IMO) is as shown in table 1.The production technology of oligoisomaltose (IMO) is roughly: the exquisite cornstarch-liquefaction-saccharification-enzyme that the goes out-essence filter-commentaries on classics glycosides-enzyme that goes out-essence filter (micro-filtration)-ion-exchange-concentrate-finished product.
The basic composition of table 1 different purity oligoisomaltose (IMO)
Summary of the invention
The purpose of this invention is to provide a kind of oligoisomaltose (IMO) that contains, can promote the long-acting sour milk and the production method thereof of beneficial bacterium in the body-Bifidobacterium propagation.
Long-acting sour milk provided by the present invention, it is made by the raw material of the component that contains leavening and following weight portion:
Milk 50-100
Oligoisomaltose 0.01-20.00
Stabilizing agent 0.2-8.0.
Wherein, the content of described oligoisomaltose is preferably the 0.01-15 weight portion, is powdery or liquid, and purity is greater than 50%, pure taste, similar sucrose.
Described oligoisomaltose (IMO) is a kind of of starch sugar, and main component is by α-1, the isomaltose (IG of 6-glycosidic bond combination
2), panose (P), Isomaltotriose (IG
3) and the above (G of tetrose
N) functional oligose that is combined into.Can be divided into two kinds in oligoisomaltose slurry and oligoisomaltose powder by form.Syrup is colourless or light yellow, transparent thick liquid, and sweet taste is soft, free from extraneous odour, the visible impurity of no twenty-twenty vision.Icing Sugar is white amorphous powder, and sweet taste is soft, free from extraneous odour, the visible impurity of no twenty-twenty vision.The basic composition of different purity oligoisomaltose (IMO) is as shown in table 1.The production technology of oligoisomaltose (IMO) is roughly: the exquisite cornstarch-liquefaction-saccharification-enzyme that the goes out-essence filter-commentaries on classics glycosides-enzyme that goes out-essence filter (micro-filtration)-ion-exchange-concentrate-finished product.
Described milk is defined as: common cow's milk comprises full-cream, degreasing and half skimmed milk; Or with the formulated recombined milk of other components of milk powder, cream, protein hydrolysate, whey powder or cow's milk.
Described stabilizing agent is emulsifying agent or thickener, or is combined by emulsifying agent and thickener, and wherein, the ratio of weight and number of emulsifying agent and thickener is 1:1-60; Described emulsifying agent can be selected from a kind of or its several combinations in single stearic fat glyceride, sucrose fatty ester, fatty acid glyceride and the polyglycerol ester; Thickener can be selected from a kind of or its several combinations in sodium carboxymethylcellulose, carragheen, gelatin, microcrystalline cellulose, gellan gum, xanthans, propylene glycol alginate, konjac glucomannan, starch, albumen powder, guar gum, D-glucose, agar, locust bean gum and the pectin.
Bacterial classification content can be 10 in the described leavening
6-10
8The CFU/100g raw material.
One or more the combination of the optional streptococcus thermophilus of described bacterial classification, streptococcus lactis, streptococcus cremoris, lactobacillus acidophilus, lactobacillus bulgaricus, missible oil streptococcus, the bacterium that produces aromatic substance, lemon leukonid, the bright string strain of pentose bacterium, diacetyl streptococcus lactis, thermophilic lactobacillus, Lactobacillus casei, Bifidobacterium, bifidobacterium bifidum, bifidobacterium longum, bifidobacterium infantis, bifidobacterium breve.
In order to improve the mouthfeel of sour milk, can contain 0-0.2 weight portion acidity regulator in the described long-acting sour milk, described acidity regulator can be the one or more combination in lactic acid, citric acid, phosphoric acid, tartaric acid and malic acid and the salt thereof.
In order to improve the mouthfeel of sour milk, contain white granulated sugar and the 0-5 weight portion syrup or the sweetener of 3.0-15.0 weight portion in the described long-acting sour milk.Described sweetener can be acesulfame-K, Aspartame, sweet, the Sucralose of knob, and described syrup can be the one or more combination in fructose and the HFCS.Conversion is the white granulated sugar pol when calculating pol.
Abundanter for the nutrition that makes sour milk, can contain the trace element of fruit juice, jam, fruit grain, nut granule or the 0.01-0.5 weight portion of 0-20 weight portions in the described long-acting sour milk.
Described fruit grain can be the one or more combination in the various fruit-vegetable granules such as tomato particle, peach particle, coconut palm fruit granule, orange fruit grain, mango fruit grain, grape fruit, pawpaw fruit grain, pineapple fruit grain, strawberry fruit grain, celery particle, the operatic circle grain, cucumber particle, radish kind particle and aloe gel particle; Usually select granular garden stuff grain or the slurry class fruit and vegetable of diameter 1-20mm for use, or length is that 1-20mm, width are the fibrous fruit and vegetable of 1-10mm.
Described fruit juice can be the one or more combination in the various Juices such as cider, strawberry juice, blueberry juice, Chinese flowering quince juice, bananas juice, orange juice, pineapple juice, mango juice, tomato juice, carrot juice, asparagus juice, spinach juice, peach juice, apricot juice, pear juice, grape juice, asparagus juice, kiwi-fruit juice, Lychee juice, lemon juice and Coconut Juice.
Described jam can be the one or more combination in the various jam such as apple jam, strawberry jam, blueberry sauce, pawpaw sauce, banana sauce, orange sauce, pineapple jam, mango chutney, catsup, carrot sauce, asparagus sauce, spinach sauce, peach butter, apricot sauce, pears sauce, grape sauce, aloe paste, kiwi-fruit jam, lichee sauce, jam with lemon and coconut palm sauce.
Nut granule is selected one or more of peanut particle, fibert particle, walnut particle, sunflower seeds particle, almond particle, American pistachios particle for use.It is the spherical particle of 1-18mm that particle is selected diameter for use, and perhaps length is 1-20mm, the particle of width 1-12mm.
Described trace element can be vitamin A, vitamin B complex, vitamin D, vitamin C, vitamin E, calcium lactate, taurine, folic acid, nicotinic acid, choline, ferrous sulfate, ironic citrate, zinc sulfate, zinc gluconate, sodium selenite, magnesium sulfate, magnesium gluconate and l-cn etc. to the one or more combination in human body beneficial's the trace element.
In order to improve the local flavor of sour milk, also contain the flavoring essence of 0.01-0.4 weight portion in the described long-acting sour milk, described flavoring essence can be sour milk essence, milk flavour, orange essence, grape essence, peach essence, cocoanut flavour, strawberry essence, lychee flavor, flavoring apple essence, close melon essence, mango essence, passionflower essence, grapefruit essence, lemon extract, blueberry essence, pawpaw essence, flavoring banana essence, flavoring pineapple essence, tomato flavour, carrot essence, asparagus essence, spinach essence, apricot essence, pear essence, kiwi flavor, vanilla, flavoring rose essence, chocolate essence, walnut essence, one or more combination in wheat essence and the cherry essence.
The present invention contains the long-acting sour milk production method of beneficial bacteria factor, comprises the steps:
May further comprise the steps:
1) with sweetener, oligoisomaltose and the stabilizing agent of described weight portion, mixes with milk;
2) carrying out sterilization, cooling, the described leavening of adding ferments;
3) acidity regulator, flavoring essence and/or the sweetener of the described weight portion of interpolation after the fermentation ends, sterilization obtains long-acting sour milk.
Long-acting sour milk of the present invention contains oligoisomaltose (IMO), can promote beneficial bacterium in the body-Bifidobacterium propagation.
Long-acting sour milk of the present invention is compared with existing like product, has the following advantages and useful effect: 1) the present invention has improved the quantity (being primarily aimed at the acid strong crowd of hydrochloric acid in gastric juice) of Bifidobacterium in the human body; 2) this product has been broken through original sour milk products structure and prescription composition, has increased the new varieties of product; 3) this product has not only kept the nutritional labeling of sour milk, the shelf-life of product was reached more than two months, enlarged the sales range of product, thereby made the people that are in the mountain village that the geographical position is remote, communications and transportation is inconvenient can enjoy multiple tastes, be of high nutritive value and drink yoghurt easily.4) interpolation of fruit juice, jam, fruit grain or nut granule has realized that fruit vitamin, mineral matter, cellulose combine with milk protein, make the nutrition of product more reasonable, more balanced; 5) fruit juice, jam or the interpolation of fruit grain in long-acting sour milk have promoted the development of fruits and vegetables industries, and the fruit resource of China's abundant is fully used, and its economic benefit and social benefit are very considerable.
The specific embodiment
Method therefor is conventional method if no special instructions among the following embodiment, and described degree is the quality percentage composition if no special instructions.
The preparation of embodiment 1, beneficial bacteria factor long-acting sour milk
Raw material:
Milk: protein: 2.98%, fat: 3.02%, non-fat solid: 8.55%.
White granulated sugar: meet country-level standard.
HFCS: F 〉=42.0%, 5%.
Sucrose fatty ester: Mitsubishi, HLB are 7.
Strawberry essence: green brilliant spices Co., Ltd, model: k001.
Starch: Co., Ltd is made a concerted effort to tie up in Guangzhou, model: M98.
Agar: Co., Ltd is made a concerted effort to tie up in Guangzhou, model: H65.
Oligoisomaltose (IMO): IMO 〉=50%, available from Shandong Baolingbao Biotechnology Co., Ltd., model: FM500, for powdery.
Leavening: Sha Pu looks trade Co., Ltd's (model: OI654 is made up of streptococcus thermophilus and lactobacillus bulgaricus, and the ratio of its viable count is 4:6).
Pure water, lactic acid and citric acid all meet national standard.
Prepare beneficial bacteria factor long-acting sour milk of the present invention, may further comprise the steps:
1) gets material (in 1 ton) by following composition of raw materials: 700.0 kilograms in milk, 95.0 kilograms of white granulated sugars, 50.0 kilograms of HFCSs, 18 kilograms of oligoisomaltoses (IMO), 1.6 kilograms in agar, 3.2 kilograms of sodium carboxymethylcelluloses, 10.0 kilograms of starch, 2 kilograms of sucrose fatty esters, leavening 10
8CFU/100g, 0.3 kilogram of strawberry essence, 119.4 kilograms of pure water
2) agar, sucrose fatty ester, sodium carboxymethylcellulose and starch and white granulated sugar are mixed, be heated in 60 ℃ the milk, insulation dissolving 25 minutes is cooled to 30 ℃ through cold drawing, add again and fill in the material-compound tank of HFCS, oligoisomaltose (IMO) and water fully stirring.
3) being warmed up to 40 ℃, pressure is under the 25Mp mixed liquor to be carried out homogeneous.
4) feed liquid of step 3) is carried out sterilization in 92 ℃, 5 minutes.
5) add bacterial classification after being cooled to 37 ℃ after the sterilization, fermented 7 hours.
6) fermentation back adding strawberry essence stirs, and after stirring, carries out 70 ℃, and sterilization in 10 minutes is cooled to 30 ℃, sterile filling.
This sour milk contains beneficial bacteria factor (oligoisomaltose (IM0), it detects the check according to QB/T2491) can improve intestinal environment, promotes the growth of beneficial bacterium.The metabolite that also has in the sweat in this sour milk simultaneously to be produced helps absorption of human body, makes the nutrition of this sour milk abundanter.
The preparation of embodiment 2, beneficial bacteria factor long-acting sour milk
Raw material:
Milk: protein: 3.06%, fat: 3.13%, non-fat solid: 8.59%.
Albumen powder: WPC is 50, and dosage is 20 kilograms, and the synthetic industry in Guangzhou has Co., Ltd, model: N487.
White granulated sugar: meet country-level standard.
Oligoisomaltose (IMO): IM0 〉=50%, available from Shandong Baolingbao Biotechnology Co., Ltd., model is Q756.
Leavening: Sha Pu looks trade Co., Ltd's (model: 0I654 is made up of streptococcus thermophilus and lactobacillus bulgaricus, and the ratio of its viable count is 4:6).
Fruit juice: inspissated juice, apple juice system pH3.7, pol 35BX.
Pectin: Shanghai shuttle ocean food Development stock Co., Ltd, model: E102; Propylene glycol alginate: the inferior methylene blue in Beijing Trade Development Co., Ltd of speeding, model: R203; Gelatin: go up the special food science and technology of Haikang Mil Co., Ltd, model: T105; Locust bean gum: the Co., Ltd of trade already, model: T302 are believed in the Qinhuangdao; Flavoring apple essence: limit, field, Shanghai spices Co., Ltd, model w100.
Pure water meets national standard.
Be prepared as follows long-acting sour milk:
1) gets material (in 1 ton) by following composition of raw materials: 500.0 kilograms in milk, 34 kilograms of whole milk powders, 2.0 kilograms of albumen powders, 95.0 kilograms of white granulated sugars, 15.0 kilograms of oligoisomaltoses (IMO), 15 kilograms of fruit juice, 3.0 kilograms of pectin, 1.0 kilograms of propylene glycol alginates, 4.0 kilograms in gelatin, 0.5 kilogram of locust bean gum, leavening 10
7CFU/100g, 2.0 kilograms of lactic acid, 0.1 kilogram of citric acid, 0.1 kilogram of natrium citricum, 0.1 kilogram of flavoring apple essence, 328.2 kilograms of pure water;
2) pectin, locust bean gum, gelatin and propylene glycol alginate and white granulated sugar are mixed, be heated in 60 ℃ the milk, insulation dissolving 20 minutes, other gets 200 kilograms pure water and is warmed up to 55 ℃, add whole milk powder and albumen powder, oligoisomaltose (IMO), dissolved 15 minutes, more than both mix.Be cooled to 25 ℃ through cold drawing, stir after adding fruit juice, essence constant volume again.
3) being warmed up to 70 ℃, pressure is under the 20Mpa mixed liquor to be carried out homogeneous.
4) feed liquid of step 3) is carried out 85 ℃, sterilization in 4 minutes.
5) be cooled to 40 ℃ after the sterilization and add bacterial classification, fermented 6 hours.
6) feed liquid that ferments is temporarily stored in the surge tank, will adds in the material-compound tank with acidity regulator (lactic acid, natrium citricum and the citric acid) spray that normal temperature pure water (20 ℃) fully dissolves at last, continue in the spray process to stir, must not be interrupted, stop.Add flavoring apple essence, stir, 70 ℃, carried out sterilization, and carried out sterile filling then in 15 minutes.
This sour milk contains beneficial bacteria factor, and (oligoisomaltose (IMO), detection method is the same.), can improve intestinal environment, promote the growth of beneficial bacterium.The metabolite that also has in the sweat in this sour milk simultaneously to be produced helps absorption of human body.In addition, also add and go up Juice, contain a large amount of vitamin and dietary fiber in it, make the nutrition of this sour milk abundanter.
The preparation of embodiment 3, beneficial bacteria factor long-acting sour milk
Raw material:
Milk: protein: 2.95%, fat: 3.0%, non-fat solid: 8.5%.White granulated sugar: meet country-level standard.
Oligoisomaltose (IMO): IMO 〉=50%, available from Shandong Baolingbao Biotechnology Co., Ltd., model: Y001.
Leavening: Sha Pu looks trade Co., Ltd's (model: OI654 is made up of streptococcus thermophilus and lactobacillus bulgaricus, and the ratio of its viable count is 4:6).
Gellan gum: the new hundred border industry and trade Co., Ltds that benefit the nation, model: U405; Xanthans: the new hundred border industry and trade Co., Ltds that benefit the nation, model: I405; Konjac glucomannan: port collection Group Co.,Ltd, model: 0405; Dietary fiber: water insoluble dietary fiber 〉=60%.The new hundred border industry and trade Co., Ltds that benefit the nation, model: P405; Mango essence: laugh at and see well spices Co., Ltd, model: A498; Strawberry fruit grain: flesh-content 〉=55.0%; The fruit particle size is 2-5mm; The fruit plastochondria is pH3.6; Pol 20BX.
Pure water meets national standard.
Be prepared as follows long-acting sour milk:
1) gets material (in 1 ton) by following composition of raw materials: 800.0 kilograms in milk, leavening 0.5 * 10
8CFU/100g
, 90.0 kilograms of white granulated sugars, 25 kilograms of oligoisomaltoses (IMO), 0.15 kilogram of gellan gum, 2.0 kilograms of xanthans, 1.0 kilograms of konjac glucomannans, 1.0 kilograms in mango essence, 5.0 kilograms of dietary fibers, 5.0 kilograms of fruit grains, 70.85 kilograms of pure water;
2) gellan gum, xanthans, konjac glucomannan and white granulated sugar are mixed, be heated in 65 ℃ the milk, insulation dissolving 20 minutes is cooled to 28 ℃ through cold drawing, adding fills in the material-compound tank of oligoisomaltose (IMO), dietary fiber again, fully mixes.
3) being warmed up to 55 ℃, pressure is under the 17Mpa mixed liquor to be carried out homogeneous.
4) feed liquid of step 3) is carried out sterilization in 109 ℃, 8 minutes.
5) be cooled to 38 ℃ after the sterilization and add bacterial classification, fermented 6.6 hours.
6) feed liquid adding fruit grain and the mango essence that ferments is carried out 70 ℃, 20 minutes sterilizations, sterile fillings then.
This sour milk contains beneficial bacteria factor (oligoisomaltose (IMO), the method for detection is the same), can improve intestinal environment, promotes the growth of beneficial bacterium.The metabolite that also has in the sweat in this sour milk simultaneously to be produced helps absorption of human body.In addition, also add and go up the fruit grain, contain a large amount of vitamin and dietary fiber in it, make the nutrition of this sour milk abundanter.
The preparation of embodiment 4, beneficial bacteria factor long-acting sour milk
Raw material:
Milk: protein: 3.25%, fat: 3.08%, non-fat solid: 8.56%.White granulated sugar: meet country-level standard.
Oligoisomaltose (IMO): IMO 〉=50%, available from Shandong Baolingbao Biotechnology Co., Ltd., model: S205 is for liquid.
Leavening: Sha Pu looks trade Co., Ltd's (model: OI654 is made up of streptococcus thermophilus and lactobacillus bulgaricus, and the ratio of its viable count is 4:6).
Calcium lactate: meet national standard.
Pulp: strawberry pulp, pulp system pH3.7, pol 35BX.
Acesulfame potassium: commerce and trade Co., Ltd of the excellent power in Beijing section, model: D452; Aspartame: commerce and trade Co., Ltd of the excellent power in Beijing section, model: D584; Gellan gum: Chengdu Hua Tong Food Co., Ltd, model: G648; Carragheen: Chengdu Hua Tong Food Co., Ltd, model: H648; Single stearic fat glyceride: the Inner Mongol hundred million sharp Bioisystech Co., Ltd, model: J456; Strawberry essence: Yinxiang Biological Engineering Co., Ltd., Zhejiang Prov, L684; Pure water all meets national standard.
Be prepared as follows long-acting sour milk:
1) get material (in 1 ton) by following composition of raw materials: milk: 893.5 kilograms, leavening 0.8 * 108CFU/100g, white granulated sugar: 60.0 kilograms, acesulfame potassium: 0.06 kilogram, Aspartame: 0.004 kilogram, oligoisomaltose (IMO): 30.0 kilograms, gellan gum: 0.5 kilogram, sodium carboxymethylcellulose: 2.5 kilograms, 1.0 kilograms of carragheens, 0.8 kilogram of single stearic fat glyceride, calcium lactate: 1.0 kilograms, pulp: 10 kilograms, 0.75 kilogram of strawberry essence.
2) gellan gum, sodium carboxymethylcellulose, carragheen, single stearic fat glyceride, acesulfame potassium, Aspartame and white granulated sugar are mixed, be heated in 60 ℃ the milk, insulation dissolving 18 minutes, be cooled to 25 ℃ through cold drawing, adding fills in the material-compound tank of oligoisomaltose (IMO), pulp, calcium lactate again, fully mixes.
3) with step 2) feed liquid carry out sterilization in 77 ℃, 6 minutes.
4) be cooled to 42 ℃ after the sterilization and add bacterial classification, fermented 5 hours.
5) fermentation adding essence stirs, and carries out sterilization in 68 ℃, 25 minutes, sterile filling.
This sour milk contains beneficial bacteria factor (oligoisomaltose (IMO), the method for detection is the same), can improve intestinal environment, promotes the growth of beneficial bacterium.The metabolite that also has in the sweat in this sour milk simultaneously to be produced helps absorption of human body.In addition, also add and go up jam, contain a large amount of vitamin and dietary fiber in it, make the nutrition of this sour milk abundanter.
Embodiment 5, oligoisomaltose (IMO) are regulated the zoopery of gut flora function
Given the test agent: oligoisomaltose (IMO), available from the precious company of Shandong bowling, purity 50% is white powder.Preserve shady and cool, dry, ventilation.
Animal used as test: select the 18-22g available from Institute of Experimental Animals, Chinese Academy of Medical Sciences (credit number: SCXK-(capital) 2004-0001) for use, 48 of the healthy cleaning level of BALB/C male mices are divided into four groups, 12 every group.
Dosage: the recommended amounts of oligoisomaltose (IMO) is adult (pressing the 60kg batheroom scale) 10g every day, 20g and 30g.According to three RDs 10 times are provided with mouse experiment dosage respectively, and promptly every day, dosage was respectively 1.67g/kgBW (low dose group), 3.33g/kgBW (middle dosage group), 5.00g/kgBW (high dose group).Tried thing water (sterilizing) preparation, per os gives mouse once a day and is tried thing, and the continuous irrigation stomach is tested every index after 14 days.The mouse stomach volume is heavy for the 0.10mL/10g mouse.Establish blank group (0g/kgBW) simultaneously, water (sterilizing) replaces being tried thing, and it is long-pending with respectively to be tried the thing group identical to irritate body of stomach every day.
Before giving given the test agent, the aseptic stool in mice number got is put in the aseptic vessel, uses the assay balance weighing, record weight.Then, in clean bench, sterile working adds dilution (redistilled water), is diluted to 10
-2, the mixing that fully vibrates is according to 10 times of serial dilutions to 10
-8, to measure bacterium for every kind and select suitable dilution factor, inoculation is dull and stereotyped, detects the flora quantity of five kinds of typical bacterium of enteron aisle (Bifidobacterium, lactobacillus, enterococcus, enterobacteria, C.perfringens).Culture medium, cultivation and authentication method are as shown in table 1, calculate the wet clump count (cfu/g) in just of every gram then, except that C.perfringens, all take the logarithm and carry out statistical disposition.Give given the test agent 24h afterwards the last time, detect the flora quantity of five kinds of typical bacterium of above-mentioned enteron aisle once more, method step is the same.
Data are handled: with SPSS software the initial data of each experimental group is carried out data and handle, adopt the program of variance analysis to carry out homogeneity test of variance earlier, variance is neat, calculates the F value, F value＜F
0.05, conclusion: each organizes the mean differences does not have conspicuousness; F value 〉=F
0.05, P≤0.05 is added up with the comparative approach in twos of mean between a plurality of experimental group and control group; The data of Non-Gaussian Distribution or heterogeneity of variance are carried out the conversion of suitable variable, wait to satisfy normal state or variance are neat require after, add up with the data after changing; If still for to reach normal state or the neat purpose of variance, use rank test instead and add up after the variable conversion.
The result judges: last, criterion according to " health food check and assessment technique standard " (version in 2003), Bifidobacterium between comparative experiments front and back self and group, lactobacillus, enterococcus, enterobacteria, the number change situation of C.perfringens, experimental group experiment front and back self comparing difference has conspicuousness, or comparing difference has conspicuousness between experiment back experimental group and control group group, and experimental group experiment front and back self comparing difference has conspicuousness, meet with the next item down, can judge this group given the test agent zoopery positive as a result: Bifidobacterium and/or lactobacillus obviously increase in (1) ight soil, C.perfringens reduces or does not increase enterobacteria, enterococcus does not have significant change.(2) Bifidobacterium and/or lactobacillus obviously increase in the ight soil, and C.perfringens reduces or do not increase, and enterobacteria and/or enterococcus obviously increase, but the amplitude that increases is lower than the amplitude that Bifidobacterium/lactobacillus increases.
Oligoisomaltose (IMO) is as shown in table 3 to the testing result of mouse body weight influence, initial body weight of mouse and experiment back body weight compare in each dosage group and 0g/kgBW group, there are no significant for difference (P〉0.05), show that oligoisomaltose (IMO) has no adverse effects to the body weight of mouse.
The testing result of enterobacteria flora quantity is as shown in table 4 in the mouse intestinal of experiment front and back, give and tried to compare between preceding each dosage group of thing and 0g/kgBW group, the quantity of enterobacteria does not all have significant difference (P〉0.05), and compared with the 0g/kgBW group after 14 days to trying thing, 5.00g/kgBW the quantity of group enterobacteria reduces, there were significant differences (P＜0.05).Each dosage group self enterobacteria quantity there are no significant difference (P〉0.05) relatively before and after the experiment.
The testing result of enterococcus flora quantity is as shown in table 5 in the mouse intestinal of experiment front and back, give and tried to compare between preceding each dosage group and 0g/kgBW group, enterococcal quantity there are no significant difference (P〉0.05), compared with the 0g/kgBW group after 14 days to trying thing, reduce 5.00g/kgBW organize enterococcal quantity, there were significant differences (P＜0.05).Each dosage group self enterococcus there are no significant difference (P〉0.05) relatively before and after the experiment.
The testing result of lactobacillus flora quantity is as shown in table 6 in the mouse intestinal of experiment front and back, and give and tried to compare between forward and backward each dosage group of thing and 0g/kgBW group, the quantity there was no significant difference of lactobacillus (P〉0.05).Give and to be tried forward and backward each dosage group of thing self relatively, the quantity of lactobacillus there are no significant difference (P〉0.05).
Before and after the experiment in the mouse intestinal testing result of C.perfringens flora quantity as shown in table 7, give and tried to compare between forward and backward each dosage group and 0g/kgBW group, the quantity of C.perfringens there are no significant difference (P〉0.05).Give and to be tried respectively to organize self relatively before and after the thing, the quantity of C.perfringens does not all have significant difference (P〉0.05).
Before and after the experiment in the mouse intestinal testing result of Bifidobacterium flora quantity shown in table 8 and table 9, as shown in Table 8, give and tried to compare between forward and backward each dosage group and 0g/kgBW group, the quantity of Bifidobacterium there are no significant difference (P〉0.05).Give tried thing before and after 0g/kgBW, 1.67g/kgBW group through self relatively, the quantity of Bifidobacterium there are no significant difference (P〉0.05); 3.33g/kgBW organize self relatively, the quantity of Bifidobacterium increases, and significant difference (P＜0.05) is arranged; 5.00g/kgBW experimental group self relatively, the quantity of Bifidobacterium increases, and significant difference (P＜0.01) is arranged.As shown in Table 9, give and to be tried comparison between forward and backward each dosage group and 0g/kgBW group, the quantity of Bifidobacterium there are no significant difference (P〉0.05).Give and tried thing front and back 3.33g/kgBW group self relatively, the quantity of Bifidobacterium increases by 1.4 times; 5.00g/kgBW organize self relatively, the quantity of Bifidobacterium increases by 2.0 times.
In sum, per os gives mouse oligoisomaltose (IMO) 14 days front and back, and the 3.33g/kgBW group self compares, and the quantity of Bifidobacterium increases by 1.4 times in the mouse intestines, and significant difference (P＜0.05) is arranged; 5.00g/kgBW organize self relatively, the quantity of Bifidobacterium increases by 2.0 times, and significant difference (P＜0.01) is arranged.Per os gave mouse oligoisomaltose (IMO) after 14 days, compared with the 0g/kgBW group, and 5.00g/kgBW group enterobacteria quantity reduces (P＜0.05); Enterococcus quantity significantly reduces (P＜0.05).Give before and after the oligoisomaltose (IMO), each organizes mouse C.perfringens quantity does not all have marked change.Oligoisomaltose (IMO) has no adverse effects to the mouse body weight.
Above-mentioned experimental result shows that it is positive that oligoisomaltose (IMO) is regulated the gut flora contractile studies, proves that oligoisomaltose (IMO) can the had significant proliferation intestinal bifidobacteria.
The check of table 2 gut flora culture medium, cultivation and authentication method
| The bacterium name | Culture medium | Condition of culture | Authentication method |
| Enterobacteria | Eosin methylene blue agar (Qingdao Hai Bo Bioisystech Co., Ltd) | 36±1℃，24h | Counting lactose fermenters, dyeing microscopic examination are G -All bacterium colonies of bacillus |
| Enterococcus | Sodium azide-crystal violet-aesculin agar (Qingdao Hai Bo Bioisystech Co., Ltd) | 36±1℃，48h | It is G that counting has obvious brown circle, dyeing microscopic examination +All bacterium colonies of coccus |
| Lactobacillus | Lactobacillus selective medium (Qingdao Hai Bo Bioisystech Co., Ltd) | 36±1℃，48h | According to GB/T4789.34-2003 |
| Bifidobacterium | Bifidobacterium selective medium (Qingdao Hai Bo Bioisystech Co., Ltd) | Anaerobism is cultivated 36+1 ℃, 48h | G +Sporeless bacterium, the negative APICH50 of catalase |
| C.perfringens | C.perfringens selective medium (Qingdao Hai Bo Bioisystech Co., Ltd) | Anaerobism is cultivated 36+1 ℃, 24h | Count all have fluorescence under ultraviolet light black bacterium colony |
Respectively organize the body weight (x ± SD) of mouse before and after table 3 experiment
| Group | Number of animals (only) | Body weight (g) before the experiment | With 0g/kgBW group P value relatively | Experiment back body weight (g) | With 0g/kgBW group P value relatively |
| 0g/kgBW | 12 | 21.4±0.9 | ...... | 26.6±1.2 | ...... |
| 1.67g/kgBW | 12 | 21.1±1.3 | 0.895 | 25.5±1.7 | 0.156 |
| 3.33g/kgBW | 12 | 21.4±1.1 | 1.000 | 26.3±1.8 | 0.890 |
| 5.00g/kgBW | 12 | 21.4±0.8 | 1.000 | 25.4±1.2 | 0.137 |
Testing result (the log of enterobacteria flora quantity in table 4 mouse intestinal
10Cfu/g, x ± SD)
| Group | Number of animals (only) | Give and tried thing | With 0g/kgBW group P value relatively | To after being tried thing | With 0g/kgBW group P value relatively | This group is given the P value of being tried the thing front and back |
| 0g/kgBW | 12 | 5.97±0.27 | ...... | 6.32±0.4 | ...... | 0.062 |
| 1.67g/kgBW | 12 | 6.14±0.31 | 0.338 | 6.12±0.31 | 0.271 | 0.751 |
| 3.33g/kgBW | 12 | 6.09±0.24 | 0.635 | 6.04+0.27 | 0.087 | 0.587 |
| 5.00g/kgBW | 12 | 6.07±0.33 | 0.763 | 5.96±0.23 a | 0.021 | 0.207 |
A: relatively there were significant differences with the 0g/kgBW group
Enterococcus flora quantity testing result (log in table 5 mouse intestinal
10Cfu/g, x ± SD)
| Group | Number of animals (only) | Give and tried thing | With 0g/kgBW group P value relatively | To after being tried thing | With 0g/kgBW group P value relatively | This group is given the P value of being tried the thing front and back |
| 0g/kgBW | 12 | 6.98±0.30 | ...... | 7.29±0.61 | ...... | 0.068 |
| 1.67/kgBW | 12 | 7.04±0.38 | 0.974 | 7.11±0.39 | 0.677 | 0.546 |
| 3.33g/kgBW | 12 | 6.82±0.53 | 0.669 | 6.84±0.47 | 0.065 | 0.828 |
| 5.00g/kgBW | 12 | 8.92±0.50 | 0.971 | 6.74±0.40 a | 0.019 | 0.292 |
A: relatively there were significant differences with the 0g/kgBW group
Table 6 mouse lactobacillus flora quantity testing result (log
10Cfu/g, x ± SD)
| Group | Number of animals (only) | Give and tried thing | With 0g/kgBW group P value relatively | To after being tried thing | With 0g/kgBW group P value relatively | This group is given the P value of being tried the thing front and back |
| 0g/kgBW | 12 | 8.30±0.29 | ...... | 8.39±0.54 | ...... | 0.559 |
| 1.67g/kgBW | 12 | 8.31±0.19 | 1.000 | 8.41±0.39 | 0.999 | 0.313 |
| 3.33g/kgBW | 12 | 8.29±0.25 | 1.000 | 8.42±0.25 | 0.998 | 0.128 |
| 5.00g/kgBW | 12 | 8.28±0.50 | 0.998 | 8.56±0.30 | 0.605 | 0.120 |
Table 7 mouse C.perfringens flora quantity testing result (cfu/g, x ± SD)
| Group | Number of animals (only) | To before being tried thing | With 0g/kgBW group P value relatively | To after being tried thing | With 0g/kgBW group P value relatively | This group is given the P value of being tried the thing front and back |
| 0g/kgBW | 12 | 17±25 | ...... | 21±26 | ...... | 0.723 |
| 1.67g/kgBW | 12 | 17±33 | 1.000 | 21±26 | 1.000 | 0.674 |
| 3.33g/kgBW | 12 | 17±23 | 0.970 | 12±23 | 0.736 | 1.000 |
| 5.00g/kgBW | 12 | 17±33 | 1.000 | 12±23 | 0.736 | 0.586 |
Bifidobacterium flora quantity testing result (log in table 8 mouse intestinal
10Cfu/g, x ± SD)
| Group | Number of animals (only) | Give and tried thing | With 0g/kgBW group P value relatively | To after being tried thing | With 0g/kgBW group P value relatively | This group is given the P value of being tried the thing front and back |
| 0g/kgBW | 12 | 8.60±0.23 | ...... | 8.77±0.36 | ...... | 0.165 |
| 1.67g/kgBW | 12 | 8.59±0.36 | 1.000 | 8.81±0.36 | 0.984 | 0.062 |
| 3.33g/kgBW | 12 | 8.55±0.30 | 0.970 | 8.88±0.40 b | 0.805 | 0.040 |
| 5.00g/kgBW | 12 | 8.65±0.36 | 0.956 | 9.11±0.35 a | 0.069 | 0.001 |
B: relatively there were significant differences before being tried thing with this group
Bifidobacterium flora quantity testing result (* 10 in table 9 mouse intestinal
8Cfu/g, x ± SD)
| Group | Number of animals (only) | To before being tried thing | To after being tried thing | This group is given and is tried relatively to increase before and after the thing |
| 0g/kgBW | 12 | 4.4±1.9 | 7.5±5.2 | ---- |
| 1.67g/kgBW | 12 | 5.4±4.7 | 8.3±5.3 | ---- |
| 3.33g/kgBW | 12 | 4.5±3.9 | 10.8±9.4 | 1.4 doubly |
| 5.00g/kgBW | 12 | 5.5±2.9 | 16.5±10.6 | 2.0 doubly |
Embodiment 6, oligoisomaltose (IMO) are regulated the human experimentation of gut flora function
Given the test agent: oligoisomaltose (IMO), available from the precious company of Shandong bowling, purity 50% is white powder.Preserve shady and cool, dry, ventilation.
Experimenter group: through 60 of all normal adults of clinical health check-up index, the men and women half and half.30 constipation persons by name among the experimenter.
The enteric bacteria cultural method is as follows:
Bifidobacterium: BBL agar medium (Qingdao Hai Bo Bioisystech Co., Ltd), 37 ℃, anaerobism was cultivated in 48-72 hour;
Lactobacillus: LBs agar medium (Qingdao Hai Bo Bioisystech Co., Ltd), 37 ℃, cultivated in 48 hours;
Enterobacteria: EMB agar medium (Qingdao Hai Bo Bioisystech Co., Ltd), 37 ℃, cultivated in 24-48 hour;
Enterococcus: Sodium azide-crystal violet-aesculin agar medium (Qingdao Hai Bo Bioisystech Co., Ltd), 37 ℃, cultivated in 24-48 hour;
Bacteroid: Bd agar medium (Qingdao Hai Bo Bioisystech Co., Ltd), 37 ℃, anaerobism was cultivated in 24 hours;
C.perfringens: TSC agar medium (Qingdao Hai Bo Bioisystech Co., Ltd), 37 ℃, anaerobism was cultivated in 24 hours.
Randomly draw above-mentioned 60 routine experimenters, be divided into 3 groups (basic, normal, high dosage groups), every group 20 people, the men and women half and half.Before the test-meal given the test agent, the aseptic experimenter's ight soil of taking is put in the aseptic vessel, uses the assay balance weighing, record weight.Then, in clean bench, sterile working adds dilution (redistilled water), is diluted to 10
-2, the mixing that fully vibrates is according to 10 times of serial dilutions to 10
-8, to measure bacterium for every kind and select suitable dilution factor, inoculation is dull and stereotyped, checks above-mentioned six kinds of gut flora numbers (Bifidobacterium, lactobacillus, enterobacteria, enterococcus, bacteroid, C.perfringens).Then, basic, normal, high dosage group experimenter takes oligoisomaltose (IMO) 5g, 10g, 15g every day respectively, continuously 14d.Tried thing 3d, 7d, 10d, 14d respectively at taking, and when withdrawing back 3d, 7d, 14d, the aseptic experimenter's of taking ight soil detects and detects above-mentioned six kinds of gut flora numbers with same procedure.Observe, write down the edible front and back of experimenter subjective symptoms.
Data processing method is identical with embodiment 5.
Take 5g every day and tried thing (low dose group), take 14d, gut flora quantity testing result is as shown in table 10, and the enterobacteria of 5g/d experimental group, enterococcus, C.perfringens are taken forward and backward quantity does not have marked change (p〉0.05); Quantity increased before bacteroid 3d after taking back 10d and withdrawing took, and difference has conspicuousness (p＜0.05; Bifidobacterium is being taken back 7d, the increase of 10d quantity, and difference has conspicuousness (p＜0.05); Lactobacillus is being withdrawn back 7d quantity reduction, and difference has utmost point conspicuousness (p＜0.01).
Take every day and tried thing 10g (middle dosage group), take 14d, gut flora quantity testing result is as shown in table 11, the intestines liver bacterium of 10g/d experimental group, lactobacillus quantity no significant difference (p〉0.05); Enterococcus is being withdrawn back 14d quantity increase, and difference has conspicuousness (p＜0.05); Bacteroid wherein, is taken back 10d difference tool utmost point conspicuousness (p＜0.01) taking back 10d and withdrawing back 3d quantity increase, withdraws back 3d difference tool conspicuousness (p＜0.05); C.perfringens descends taking back 7d, 10d, 14d quantity, 10d difference tool utmost point conspicuousness (p＜0.01) wherein, 7d, 14d difference tool conspicuousness (p＜0.05); Bifidobacterium is taken back 10d difference tool utmost point conspicuousness (p＜0.01) taking back 7d, 10d and withdrawing back 7d quantity increase, takes back 7d and withdraws back 7d difference tool conspicuousness (p＜0.05).
Take every day and tried thing 15g (high dose group), take 14d, gut flora quantity testing result is as shown in table 12, and the enterococcus of 15g/d experimental group, lactobacillus quantity do not have significant variation (p〉0.05); Enterobacteria is being taken back 14d and is withdrawing back 3d quantity and reduce, and difference has conspicuousness (p＜0.05); Bacteroid 7d, 14d after taking withdraw back 7d, 14d quantity reduces, and 14d difference has utmost point conspicuousness (p＜0.01) after wherein taking back 7d and withdrawing, and taking back 14d and withdrawing back 7d difference has conspicuousness (p＜0.05); The pretty bacterium of perfringens is being taken the decline of back 7d and 10d quantity, and wherein 10d difference has utmost point conspicuousness (p＜0.01), and 7d difference has conspicuousness (p＜0.05); Bifidobacterium is being taken back 3d, the increase of 10d quantity, the equal tool utmost point of difference conspicuousness (p＜0.01)
In sum, take 14d continuously with the oligoisomaltose (IMO) of 5g/d, 10g/d, 15g/d dosage, Bifidobacterium is significantly increased.The 5g/d experimental group compares through self, and the quantity of Bifidobacterium increases by 10 times, and (P＜0.05), difference has conspicuousness; 10g/d experimental group and 15g/d experimental group compare through self, and the quantity of Bifidobacterium increases by 10 times, difference tool utmost point conspicuousness (P＜0.01).Above-mentioned experimental result shows that the Bifidobacterium quantity of each dosage group all is significantly improved, and proves that further oligoisomaltose (IMO) can obviously promote Bifidobacterium propagation in the enteron aisle.
Experimental session writes down main suit's symptom of experimenter every day, after the experimenter takes and is tried thing-oligoisomaltose (IMO) as a result, the defecation frequency rule, the ight soil proterties is normal, defecation is unobstructed, diet, sleep and the state of mind all keep well, prove that oligoisomaltose (IMO) has no adverse effects to the people, takes safety.
Table 10 is taken the oligoisomaltose human body intestinal canal flora and is dynamic observed I (5g/d) (log as a result
10CFU/g, x ± S, n=10)
| Bacterium | Enterobacteria | Enterococcus | Bacteroid | C.perfringens | Bifidobacterium | Lactobacillus |
| Before taking | 7.11±1.38 | 5.95±1.32 | 8.05±2.28 | 1.79±0.93 | 8.00±1.81 | 8.11±1.04 |
| Take back 3d | 7.53±0.97 | 5.59±1.32 | 8.06±1.95 | 1.63±0.81 | 8.29±1.76 | 8.32±0.75 |
| 7d | 7.10±1.13 | 5.72±1.67 | 8.80±2.18 | 1.81±1.00 | 9.32±0.44* | 7.97±0.80 |
| 10d | 7.32±0.85 | 5.80±1.48 | 9.74±0.82* | 1.67±1.00 | 9.50±0.63* | 7.57±1.28 |
| 14d | 7.63±0.99 | 6.24±1.03 | 9.42±0.52 | 1.42±1.40 | 8.86±0.99 | 7.99±0.62 |
| Withdraw back 3d | 7.42±0.52 | 6.27±1.74 | 9.71±0.27* | 2.53±1.22 | 8.79±0.71 | 7.95±0.63 |
| 7d | 6.57±0.86 | 5.84±1.06 | 9.46±0.66 | 2.32±1.67 | 8.60±0.76 | 7.68±0.69 |
| 14d | 7.10±0.92 | 6.17±1.25 | 7.37±2.59 | 2.49±1.44 | 8.59±0.57 | 7.31±1.59 |
* expression: take and tried behind the thing to be tried before the thing relatively p＜0.05 with taking, * * represents: take and tried behind the thing to be tried before the thing relatively p＜0.01 with taking.
Table 11 is taken the oligoisomaltose human body intestinal canal flora and is dynamic observed II (10g/d) (log as a result
10CFU/g, x ± S, n=10)
| Bacterium | Enterobacteria | Enterococcus | Bacteroid | C.perfringens | Bifidobacterium | Lactobacillus |
| Before taking | 6.73±1.37 | 5.21±1.11 | 8.03±2.18 | 2.00±0.99 | 7.75±1.58 | 7.24±1.36 |
| Take back 3d | 7.36±0.99 | 6.00±1.29 | 8.20±2.30 | 2.25±1.02 | 8.57±0.58 | 7.76±0.82 |
| 7d | 6.64±0.79 | 4.82±2.03 | 8.48±2.32 | 1.25±0.71* | 8.93±0.44* | 7.90±1.09 |
| 10d | 6.77±0.83 | 5.28±1.10 | 9.96±0.31** | 1.02±0.02** | 9.26±0.36** | 7.41±0.96 |
| 14d | 6.72±1.01 | 4.85±1.62 | 8.88±2.25 | 1.18±0.49* | 8.62±0.74 | 7.41±0.96 |
| Withdraw back 3d | 7.03±0.55 | 5.59±0.89 | 9.66±0.36* | 2.41±1.13 | 8.61±0.43 | 7.36±0.72 |
| 7d | 7.20±0.39 | 5.88±1.14 | 9.38±1.18 | 2.52±1.21 | 8.99±0.44* | 7.72±0.60 |
| 14d | 7.09±0.22 | 6.51±1.20* | 8.47±2.00 | 1.63±0.97 | 8.73±0.61 | 7.61±0.73 |
* expression: take and tried behind the thing to be tried before the thing relatively p＜0.05 with taking, * * represents: take and tried behind the thing to be tried before the thing relatively p＜0.01 with taking.
Table 12 is taken the oligoisomaltose human body intestinal canal flora and is dynamic observed III (15g/d) (log as a result
10CFU/g, x ± S, n=10)
| Bacterium | Enterobacteria | Enterococcus | Bacteroid | C.perfringens | Bifidobacterium | Lactobacillus |
| Before taking | 7.72±0.67 | 5.38±0.64 | 9.91±0.24 | 2.59±1.41 | 8.69±0.46 | 7.68±1.48 |
| Take back 3d | 7.76±1.00 | 7.01±2.07 | 9.95±0.26 | 2.65±1.47 | 9.27±0.25** | 8.24±1.69 |
| 7d | 7.18±0.93 | 5.38±1.47 | 7.87±2.07** | 1.55±0.82* | 9.06±0.66 | 7.78±0.92 |
| 10d | 7.56±0.86 | 6.41±1.38 | 9.88±0.40 | 1.24±0.69** | 9.67±0.50** | 8.21±0.86 |
| 14d | 7.18±0.59* | 5.86±1.43 | 9.64±0.30* | 1.88±1.14 | 8.88±0.52 | 7.91±0.34 |
| Withdraw back 3d | 6.95±0.88* | 5.60±1.56 | 9.10±1.93 | 2.08±1.31 | 8.92±0.20 | 7.58±0.98 |
| 7d | 7.42±0.71 | 6.28±1.62 | 9.60±0.46* | 1.99±1.52 | 8.99±0.47 | 7.92±0.85 |
| 14d | 7.20±0.43 | 6.51±1.84 | 8.33±1.92** | 1.58±1.15 | 8.26±0.68 | 7.60±0.61 |
* expression: take and tried behind the thing to be tried before the thing relatively p＜0.05 with taking, * * represents: take and tried behind the thing to be tried before the thing relatively p＜0.01 with taking.
Embodiment 7 product system stable detection
Product with embodiment 1-4 is a laboratory sample, carries out static observation, the stability of testing product system under normal temperature (23-36 ℃) environmental condition.
The stability test of product system: take by weighing a certain amount of product, carry out centrifugally, directly record the amount (weight in wet base) of BSW, calculate the ratio that BSW accounts for gross weight by difference assay, thus reflection albumen precipitation and bleed situation; Observe the amount of albumen precipitation and bleed simultaneously, result such as table 13.
Table 13 product system stability observing record sheet
| Embodiment 1 | Embodiment 2 | Embodiment 3 | Embodiment 4 | |
| 7 days | Product does not have albumen precipitation and bleed, and system is stable. | Product does not have albumen precipitation and bleed, and system is stable. | Product does not have albumen precipitation and bleed, and system is stable. | Product does not have albumen precipitation and bleed, and system is stable. |
| 25 days | Product system is stable, no bleed, and sediment accounts for 0.2% | Product system is stable, no bleed, and sediment accounts for 0.16% | Product system is stable, no bleed, and sediment accounts for 0.15% | Product system is stable, no bleed, and sediment accounts for 0.22% |
| 45 days | Slight sediment is arranged, account for 0.6% of gross weight, no bleed | Slight white depositions accounts for 0.63% of gross weight, no bleed | Slight precipitation is arranged, and sediment accounts for 0.70%, no bleed | A small amount of white depositions accounts for 0.75% of gross weight, no bleed |
| 65 days | A spot of sediment accounts for 1.1% of gross weight, no bleed | A spot of sediment accounts for 1.4% of gross weight, no bleed, | Small amount of precipitate thing 1.17%, no bleed | The small amount of precipitate thing accounts for 1.35% of gross weight, no bleed, |
| 85 days | Certain sediment is arranged, account for 1.7% of gross weight, some bleeds are arranged | White depositions begins to increase, and accounts for 1.9% of gross weight, and some bleeds are arranged | Small amount of precipitate, sediment account for 2.0% of gross weight, and some bleeds are arranged | The adularescent sediment accounts for 1.95% of gross weight, and some bleeds are arranged |
| 115 days | Sediment begins to increase, and accounts for 2.8% of gross weight, and some bleeds are arranged, and can accept | White depositions begins to increase, and accounts for 3.1% of gross weight, and some bleeds are arranged, and can accept | Sediment is more obvious, accounts for 3.2% of gross weight, and some bleeds are arranged, and can accept | White depositions increases, and accounts for 3.0% of gross weight, and some bleeds are arranged, and can accept |
| 150 days | Sediment obviously occurs, and accounts for 4.1% of gross weight, and some bleeds are arranged, and can accept | White depositions is apparent in view, accounts for 4.3% of gross weight, and some bleeds are arranged, and can accept | The sediment showed increased accounts for 4.5% of gross weight, and some bleeds are arranged, and can accept | White depositions obviously increases, and accounts for 4.4% of gross weight, and some bleeds are arranged, and can accept |
| 180 days | Sediment obviously occurs, and accounts for 4.1% of gross weight, and some bleeds are arranged, and can accept | White depositions is apparent in view, accounts for 4.5% of gross weight, and some bleeds are arranged, and can accept | The sediment showed increased accounts for 4.5% of gross weight, and some bleeds are arranged, and can accept | White depositions obviously increases, and accounts for 4.4% of gross weight, and some bleeds are arranged, and can accept |
Claims (12)
1. long-acting sour milk, it is characterized in that: it is made by the raw material of the component that contains leavening and following weight portion:
Milk 50-100,
Oligoisomaltose 0.01-20.00,
Stabilizing agent 0.2-8.0,
Acidity regulator 0.2-8.0.
2. long-acting sour milk according to claim 1 is characterized in that: described milk is common cow's milk, or with the formulated recombined milk of other components of milk powder, cream, protein hydrolysate, whey powder or cow's milk;
The amount of described oligoisomaltose is that oligoisomaltose with 50% purity is a standard metering;
The content of described oligoisomaltose is for being the 0.01-15 weight portion.
3. long-acting sour milk according to claim 2 is characterized in that: described milk comprises full-cream, degreasing and half skimmed milk.
4. long-acting sour milk according to claim 3 is characterized in that: also contain Juice, jam, fruit-vegetable granules or the nut granule of 0-20 weight portion in the described raw material, or the trace element of 0.01-0.5 weight portion.
5. long-acting sour milk according to claim 4 is characterized in that: the flavoring essence and/or the white granulated sugar of 3.0-15.0 weight portion and the syrup or the sweetener of 0-5 weight portion that also contain the 0.01-0.4 weight portion in the described raw material; Described sweetener is acesulfame-K and Aspartame, and described syrup is the one or more combination in fructose and the HFCS.
6. according to the described long-acting sour milk of arbitrary claim in the claim 1 to 5, it is characterized in that: the bacterial classification content of the leavening in the described raw material is 10
6-10
8The CFU/100g raw material.
7. long-acting sour milk according to claim 6 is characterized in that: described leavening is a kind of or its any combination in streptococcus thermophilus, streptococcus lactis, streptococcus cremoris, lactobacillus acidophilus, lactobacillus bulgaricus, missible oil streptococcus, bacterium, lemon leukonid, leuconostoc dextranic, diacetyl streptococcus lactis, thermophilic lactobacillus, Lactobacillus casei, Bifidobacterium, bifidobacterium bifidum, bifidobacterium longum, bifidobacterium infantis and the bifidobacterium breve that produces aromatic substance.
8. long-acting sour milk according to claim 7 is characterized in that: described stabilizing agent is emulsifying agent or thickener, or is combined by emulsifying agent and thickener, and wherein, the ratio of weight and number of emulsifying agent and thickener is 1: 1-60; Described emulsifying agent is a kind of or its any combination in sucrose fatty ester, fatty acid glyceride and the polyglycerol ester; Described thickener is a kind of or its any combination in sodium carboxymethylcellulose, carragheen, gelatin, converted starch, albumen powder, D-glucose, microcrystalline cellulose, gellan gum, xanthans, propylene glycol alginate, konjac glucomannan, agar, locust bean gum and the pectin.
9. long-acting sour milk according to claim 8 is characterized in that: described acidity regulator is a kind of or its any combination in lactic acid, citric acid, phosphoric acid, tartaric acid and malic acid and the salt thereof.
10. long-acting sour milk according to claim 9 is characterized in that: described flavoring essence is a sour milk essence, milk flavour, orange essence, grape essence, peach essence, cocoanut flavour, strawberry essence, lychee flavor, flavoring apple essence, close melon essence, mango essence, passionflower essence, grapefruit essence, lemon extract, blueberry essence, pawpaw essence, flavoring banana essence, flavoring pineapple essence, tomato flavour, carrot essence, asparagus essence, spinach essence, apricot essence, pear essence, kiwi flavor, vanilla, flavoring rose essence, chocolate essence, walnut essence, a kind of or its any combination in wheat essence and the cherry essence.
11. long-acting sour milk according to claim 10, it is characterized in that: described fruit-vegetable granules is selected from the really combination of one or more in grain, celery particle, the operatic circle grain, cucumber particle, radish kind particle and the aloe gel particle of tomato particle, peach particle, coconut palm fruit granule, orange fruit grain, mango fruit grain, grape fruit, pawpaw fruit grain, pineapple fruit grain, strawberry, described fruit-vegetable granules is selected granular garden stuff grain or the slurry class fruit and vegetable of diameter 1-20mm for use, or length is that 1-20mm, width are the fibrous fruit and vegetable of 1-10mm; Described Juice is selected from one or more the combination in cider, strawberry juice, blueberry juice, Chinese flowering quince juice, bananas juice, orange juice, pineapple juice, mango juice, tomato juice, carrot juice, asparagus juice, spinach juice, peach juice, apricot juice, pear juice, grape juice, asparagus juice, kiwi-fruit juice, Lychee juice, lemon juice and the Coconut Juice; Described jam is selected from one or more the combination in apple jam, strawberry jam, blueberry sauce, pawpaw sauce, banana sauce, orange sauce, pineapple jam, mango chutney, catsup, carrot sauce, asparagus sauce, spinach sauce, peach butter, apricot sauce, pears sauce, grape sauce, aloe paste, kiwi-fruit jam, lichee sauce, jam with lemon and the coconut palm sauce; Described nut granule is selected one or more in peanut particle, fibert particle, walnut particle, sunflower seeds particle, almond particle, plum particle and the American pistachios particle for use, it is the spherical particle of 1-18mm that particle is selected diameter for use, perhaps length is 1-20mm, the particle of width 1-12mm;
Described trace element is selected from a kind of or its any combination in vitamin A, vitamin B complex, vitamin D, vitamin C, vitamin E, calcium lactate, taurine, folic acid, nicotinic acid, choline, ferrous sulfate, ironic citrate, zinc sulfate, zinc gluconate, sodium selenite, magnesium sulfate, magnesium gluconate and the l-cn.
12. a method of producing the described long-acting sour milk of claim 11 may further comprise the steps:
1) with sweetener, oligoisomaltose and the stabilizing agent of described weight portion, mixes with milk;
2) carrying out sterilization, cooling, the described leavening of adding ferments;
3) acidity regulator, flavoring essence and/or the sweetener of the described weight portion of interpolation after the fermentation ends, sterilization obtains long-acting sour milk.
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