CN101051023B - Detecting reagent and detecting method - Google Patents
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- CN101051023B CN101051023B CN2006100729229A CN200610072922A CN101051023B CN 101051023 B CN101051023 B CN 101051023B CN 2006100729229 A CN2006100729229 A CN 2006100729229A CN 200610072922 A CN200610072922 A CN 200610072922A CN 101051023 B CN101051023 B CN 101051023B
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- 238000000034 method Methods 0.000 title claims description 12
- 239000003153 chemical reaction reagent Substances 0.000 title claims description 9
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 54
- PXIPVTKHYLBLMZ-UHFFFAOYSA-N Sodium azide Chemical compound [Na+].[N-]=[N+]=[N-] PXIPVTKHYLBLMZ-UHFFFAOYSA-N 0.000 claims description 52
- 235000000177 Indigofera tinctoria Nutrition 0.000 claims description 43
- 229940097275 indigo Drugs 0.000 claims description 43
- COHYTHOBJLSHDF-UHFFFAOYSA-N indigo powder Natural products N1C2=CC=CC=C2C(=O)C1=C1C(=O)C2=CC=CC=C2N1 COHYTHOBJLSHDF-UHFFFAOYSA-N 0.000 claims description 43
- 229930182478 glucoside Natural products 0.000 claims description 36
- 150000008131 glucosides Chemical class 0.000 claims description 36
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims description 32
- 210000002700 urine Anatomy 0.000 claims description 32
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 29
- 239000000203 mixture Substances 0.000 claims description 28
- RBTARNINKXHZNM-UHFFFAOYSA-K iron trichloride Chemical compound Cl[Fe](Cl)Cl RBTARNINKXHZNM-UHFFFAOYSA-K 0.000 claims description 26
- 238000002835 absorbance Methods 0.000 claims description 18
- 229960000583 acetic acid Drugs 0.000 claims description 16
- 239000012362 glacial acetic acid Substances 0.000 claims description 16
- 238000010998 test method Methods 0.000 claims description 9
- 238000012360 testing method Methods 0.000 claims description 9
- ILRCGYURZSFMEG-UHFFFAOYSA-N Salidroside Natural products OC1C(O)C(O)C(CO)OC1OCCC1=CC=C(O)C=C1 ILRCGYURZSFMEG-UHFFFAOYSA-N 0.000 claims description 7
- 238000001514 detection method Methods 0.000 claims description 7
- ILRCGYURZSFMEG-RQICVUQASA-N salidroside Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)OC1OCCC1=CC=C(O)C=C1 ILRCGYURZSFMEG-RQICVUQASA-N 0.000 claims description 7
- 238000002360 preparation method Methods 0.000 claims description 5
- 238000006243 chemical reaction Methods 0.000 claims description 3
- 230000035484 reaction time Effects 0.000 claims description 3
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 10
- 102000004169 proteins and genes Human genes 0.000 description 7
- 108090000623 proteins and genes Proteins 0.000 description 7
- PCKPVGOLPKLUHR-UHFFFAOYSA-N indoxyl Chemical group C1=CC=C2C(O)=CNC2=C1 PCKPVGOLPKLUHR-UHFFFAOYSA-N 0.000 description 4
- 241000894006 Bacteria Species 0.000 description 3
- 238000004737 colorimetric analysis Methods 0.000 description 3
- 239000008367 deionised water Substances 0.000 description 3
- 229910021641 deionized water Inorganic materials 0.000 description 3
- 239000012895 dilution Substances 0.000 description 3
- 238000010790 dilution Methods 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 150000002475 indoles Chemical class 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 238000005303 weighing Methods 0.000 description 3
- KXDAEFPNCMNJSK-UHFFFAOYSA-N Benzamide Chemical compound NC(=O)C1=CC=CC=C1 KXDAEFPNCMNJSK-UHFFFAOYSA-N 0.000 description 2
- SIKJAQJRHWYJAI-UHFFFAOYSA-N Indole Chemical compound C1=CC=C2NC=CC2=C1 SIKJAQJRHWYJAI-UHFFFAOYSA-N 0.000 description 2
- 235000005911 diet Nutrition 0.000 description 2
- 230000000378 dietary effect Effects 0.000 description 2
- 210000003734 kidney Anatomy 0.000 description 2
- 210000004185 liver Anatomy 0.000 description 2
- 238000002798 spectrophotometry method Methods 0.000 description 2
- YNJBWRMUSHSURL-UHFFFAOYSA-N trichloroacetic acid Chemical compound OC(=O)C(Cl)(Cl)Cl YNJBWRMUSHSURL-UHFFFAOYSA-N 0.000 description 2
- AEMOLEFTQBMNLQ-AQKNRBDQSA-N D-glucopyranuronic acid Chemical compound OC1O[C@H](C(O)=O)[C@@H](O)[C@H](O)[C@H]1O AEMOLEFTQBMNLQ-AQKNRBDQSA-N 0.000 description 1
- IAJILQKETJEXLJ-UHFFFAOYSA-N Galacturonsaeure Natural products O=CC(O)C(O)C(O)C(O)C(O)=O IAJILQKETJEXLJ-UHFFFAOYSA-N 0.000 description 1
- QIVBCDIJIAJPQS-VIFPVBQESA-N L-tryptophane Chemical compound C1=CC=C2C(C[C@H](N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-VIFPVBQESA-N 0.000 description 1
- QIVBCDIJIAJPQS-UHFFFAOYSA-N Tryptophan Natural products C1=CC=C2C(CC(N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-UHFFFAOYSA-N 0.000 description 1
- 239000006035 Tryptophane Substances 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 230000017531 blood circulation Effects 0.000 description 1
- 238000003759 clinical diagnosis Methods 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 230000008878 coupling Effects 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
- 238000005859 coupling reaction Methods 0.000 description 1
- 238000005336 cracking Methods 0.000 description 1
- 230000002950 deficient Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 235000019621 digestibility Nutrition 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 235000006694 eating habits Nutrition 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 229940097043 glucuronic acid Drugs 0.000 description 1
- PZOUSPYUWWUPPK-UHFFFAOYSA-N indole Natural products CC1=CC=CC2=C1C=CN2 PZOUSPYUWWUPPK-UHFFFAOYSA-N 0.000 description 1
- RKJUIXBNRJVNHR-UHFFFAOYSA-N indolenine Natural products C1=CC=C2CC=NC2=C1 RKJUIXBNRJVNHR-UHFFFAOYSA-N 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 229960004799 tryptophan Drugs 0.000 description 1
- 238000011179 visual inspection Methods 0.000 description 1
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- Investigating Or Analysing Biological Materials (AREA)
Abstract
A detection reagent is prepared form concentrated sulfuric acid and sodium azide as well as ferric trichoride. The method applying said detection reagent to test indoglucoside in urine is also disclosed.
Description
Technical field
The present invention relates to a kind of detectable and detection method, particularly detect indigo glucoside detectable and detection method in the urine.
Background technology
The level of indigo glucoside has reflected the level that enteric bacteria is decomposed the accessory substance indoles of digesting protein generation in the body in the urine, if normal human's protein digestibility is incomplete, and enteric bacteria is assimilated the albumen increase, then indigo glucoside level raises, and the level of indigo glucoside has also promptly reflected the digestion situation of body internal protein in therefore urinating.The protein of not digesting and assimilating in the food is corrupt under the enteric bacteria effect can to produce many accessory substances, and wherein indoles indole is one of main accessory substance.Indoles is that the cracking tryptophane produces, and flows to liver through absorbing with blood, changes into indoxyl at liver, and glazier's salt or glucuronic acid promptly form the indigo glucoside in the indoxyl coupling, subsequently by blood flow through kidney, enter urine at kidney.
For at clinical this test item that uses better, further verify its using value in clinical diagnosis, now design the relevant clinical research project, with observe different crowd urine indigo glucoside level and with the relation of short-term dietary structure, the influence factor of analyzing urine indigo glucoside level simultaneously comprises age-sex's occupation eating habit etc., so that specify the more dietary structure of science.Present urine indigo glucoside mensuration is with the naked eye estimated urine indigo glucoside level according to the depth of color owing to employing is colourimetry, so not really accurate, the feasible accuracy of urinating indigo glucoside mensuration of detection method provided by the invention is improved.
Summary of the invention
The object of the invention is to provide indigo glucoside detectable in a kind of urine.Another purpose of the present invention is to carry
For indigo glucoside detection method in a kind of urine.
The present invention seeks to be achieved through the following technical solutions:
Detectable composition of the present invention is to be made of following component:
Concentrated hydrochloric acid: 50-120 parts by volume glacial acetic acid: 0-20 parts by volume
Ferric trichloride: 0.5-4 weight portion sodium azide: 0.05-0.2 weight portion
Water: 10-30 parts by volume
The optimum ratio of detectable composition of the present invention is as follows:
Concentrated hydrochloric acid: 70-100 parts by volume glacial acetic acid: 0-10 parts by volume
Ferric trichloride: 0.5-2 weight portion sodium azide: 0.1 weight portion
Water: 15 parts by volume
The optimum ratio of detectable composition of the present invention is as follows:
Concentrated hydrochloric acid: 60 parts by volume glacial acetic acid: 15 parts by volume
Ferric trichloride: 0.5 weight portion sodium azide: 0.15 weight portion
Water: 25 parts by volume
The optimum ratio of detectable composition of the present invention is as follows:
Concentrated hydrochloric acid: 110 parts by volume glacial acetic acid: 5 parts by volume
Ferric trichloride: 3 weight portion sodium azide: 0.1 weight portion
Water: 20 parts by volume
The optimum ratio of detectable composition of the present invention is as follows:
Concentrated hydrochloric acid: 100 parts by volume, ferric trichloride: 0.8 weight portion,
Sodium azide: 0.1 weight parts water: 15 parts by volume.
The optimum ratio of detectable composition of the present invention is as follows:
Concentrated hydrochloric acid: 80 parts by volume ferric trichlorides: 1 weight portion
Sodium azide: 0.1 weight parts water: 20 parts by volume
Detectable of the present invention, wherein various bulk drug components be directly mix or mix sequentially add all can, to close be ml/g in the unit of parts by volume/weight portion in the above-mentioned detectable.
The present invention also provides a kind of method of testing of urinating the indigo glucoside, and this method is an absorbance method, and it detects wavelength 660nm, temperature of reaction 25-37 ℃, ratio of reagents 1:1, reaction time 2-5 minute.
25 ℃ of preferred its temperature of reaction, 2 minutes reaction time.
The concrete steps of this method are as follows:
A, preparation indigo glucoside titer 200mg/L, 100mg/L, 50mg/L, 25mg/L, 12.5mg/L; Measure respectively
Draw typical curve; B, get detectable 2-5mL, temperature 25-37 ℃, wavelength 660nm place colorimetric, absorbance A 1 at the bottom of the minute book; C, get detectable 1.0-3.0mL, 1:1 adds urine specimen or standard items reagent in proportion, and mixing left standstill 2-5 minute, temperature 25-37 ℃, wavelength 660nm place colorimetric, writes down absorbance A 2; D, result calculate,
On typical curve, try to achieve corresponding indigo salidroside content;
Wherein used detectable is made by 50-120 parts by volume concentrated hydrochloric acid, 0-20 parts by volume glacial acetic acid, 0.5-4 weight portion ferric trichloride, 0.05-0.2 weight portion, sodium azide and 10-30 parts by volume water among step B, the C.
Wherein used detectable is preferably made by 70-100 parts by volume concentrated hydrochloric acid, 0-10 parts by volume glacial acetic acid, 0.5-2 weight portion ferric trichloride, 0.1 weight portion sodium azide and 15 parts by volume water among step B, the C.
Wherein preferred concentrated hydrochloric acid 60 parts by volume of used detectable, glacial acetic acid 15 parts by volume, ferric trichloride 0.5 weight portion, sodium azide 0.15 weight portion and water 25 parts by volume among step B, the C.
Wherein preferred concentrated hydrochloric acid 110 parts by volume of used detectable, glacial acetic acid 5 parts by volume, ferric trichloride 3 weight portions, sodium azide 0.1 weight portion and water 20 parts by volume among step B, the C.
The preferred concentrated hydrochloric acid of used detectable among step B, the C wherein: 100 parts by volume, ferric trichloride: 0.8 weight portion, sodium azide 0.1 weight portion, water: 15 parts by volume.
Wherein preferred concentrated hydrochloric acid 80 parts by volume of used detectable, ferric trichloride 1 weight portion, sodium azide 0.1 weight portion and water 20 parts by volume among step B, the C.
The preferred steps of said method is as follows:
A, preparation indigo glucoside titer 200mg/L, 100mg/L, 50mg/L, 25mg/L, 12.5mg/L; Measure respectively
Draw typical curve; B, get detectable 3.5mL, in 25 ℃ of temperature, wavelength 660nm place colorimetric, absorbance A 1 at the bottom of the minute book; C, get detectable 2.0mL, add urine specimen or standard items reagent 2.0mL, mixing left standstill 2 minutes, in 25 ℃ of temperature, wavelength 660nm place colorimetric, write down absorbance A 2; D, result calculate,
On typical curve, try to achieve corresponding indigo salidroside content;
Select concentrated hydrochloric acid for use in the detectable of the present invention and do not select trichloroacetic acid for use, because select for use trichloroacetic acid acidity not enough, the test of indigo glucoside just has problem.Select ferric trichloride for use in the detectable of the present invention and do not select diformazan aminophenyl formaldehyde for use, because diformazan aminophenyl formaldehyde is different with the ferric trichloride oxidability.
Use detectable of the present invention and spectrophotometric determination absorbance, calculate the content of indigo glucoside, reflected the truth of indigo glucoside in the urine by typical curve, therefore more direct reliable.Simultaneously also can get rid of the organic interference of protide, overcome with other method, cause subjective error easily, can only be used to judge approximate range because of detecting by an unaided eye, and can not be quantitative.Owing to contain protein matter in the urine, when detecting, detect thing sex change muddiness simultaneously, also can disturb the judgement of color.
Following experimental example is used to further specify but is not limited to the present invention.
The comparative experiments of experimental example 1 test urine indigo glucoside
The operation steps of urine indigo glucoside method of testing A: (1) urine specimen 2mL, (2) oxygenant 2mL, (3) mixing left standstill 2 minutes.(4) add chloroform 1mL, (5) put upside down mixing, and left standstill 2-5 minute (6), (7) visual inspection chloroform layer color.
58 parts of the clinical urine specimens of picked at random, with method of testing A (being colorimetric method) test, the shade of record chloroform layer, very shallow blue look or almost colourless negative.Darker blue look or darker color are judged to the positive.
The operation steps of urine indigo glucoside method of testing B: (using the check and analysis urine indigo glucoside of detectable of the present invention)
(1) take by weighing indigo glucoside standard items 2.0ug with electronic balance, be dissolved in the 10ml deionized water, be made into 200mg/L concentration, doubling dilution becomes 100mg/L, 50mg/L, 25mg/L, 12.5mg/L successively; Measure respectively
Draw typical curve with coordinate paper; (2) get detectable 3.5mL, in 25 ℃ of temperature, wavelength 660nm place colorimetric, absorbance A 1 at the bottom of the minute book; (3) get detectable 2.0mL, add urine specimen or standard items reagent 2.0mL, mixing left standstill 2 minutes, in 25 ℃ of temperature, wavelength 660nm place colorimetric, and record absorbance A 2; (4) result calculates,
On typical curve, try to achieve corresponding indigo salidroside content.
The result of method of testing A shows in the range of normal value (≤40.0mg/L) number 19 people, 40.0-100.0mg/L number 18 people, 〉=100mg/L number 21 people; The result of method of testing B shows normal 23 people, 14 people that exceed standard, severe overweight 21 people.
Method of testing A (with the method for chloroform extracting, i.e. colourimetry) because detect by an unaided eye, causes subjective error easily, can only be used to judge approximate range, be normal, exceed standard, severe overweight, can not be quantitative.Owing to contain protein matter in the urine, sex change muddiness under the effect of chloroform is easily disturbed the judgement of color simultaneously.During the measurement standard product, owing to there is not protein matter to disturb, effect is relatively just much better.
And method of testing B spectrophotometric determination absorbance, calculate the content of indigo glucoside by typical curve, reflected the truth of indigo glucoside in the urine, changed the defective that method A only can qualitative detection, can detect quantitatively, simultaneously also can get rid of the organic interference of protide, make testing result more objective and accurate.
The concrete experimental example of the present invention is as follows:
Embodiment 1:
Concentrated hydrochloric acid: 70-100ml, ferric trichloride: 0.5-2g, sodium azide: 0.1g, water: 15ml, mix detectable.
Embodiment 2:
Concentrated hydrochloric acid: 60ml, glacial acetic acid: 15ml, ferric trichloride: 0.5g, sodium azide: 0.15g, water: 15ml, mix detectable.
Embodiment 3:
Concentrated hydrochloric acid: 110ml, glacial acetic acid: 5ml, ferric trichloride: 3g, sodium azide: 0.1g, water: 25ml, mix detectable.
Embodiment 4:
Concentrated hydrochloric acid: 80ml, ferric trichloride: 1g, sodium azide: 0.1g, water: 15ml, mix detectable.
Embodiment 5:
Concentrated hydrochloric acid: 80ml, glacial acetic acid: 2ml, ferric trichloride: 0.5-2g, sodium azide: 0.1g, water: 15ml, mix detectable.
Embodiment 6:
Concentrated hydrochloric acid: 90ml, ferric trichloride: 0.5-2g, sodium azide: 0.1g, water: 15ml, mix detectable.
Embodiment 7:
Concentrated hydrochloric acid: 100ml, ferric trichloride: 0.8g, sodium azide: 0.1g, water: 15ml, mix detectable.
Embodiment 8: test urine indigo glucoside
(1) take by weighing indigo glucoside standard items 2.0ug with electronic balance, be dissolved in the 10ml deionized water, be made into 200mg/L concentration, doubling dilution becomes 100mg/L, 50mg/L, 25mg/L, 12.5mg/L successively; Measure respectively
Draw typical curve with coordinate paper; (2) get the detectable 3.5mL of embodiment 1, in 30 ℃ of temperature, wavelength 660nm place colorimetric, absorbance A 1 at the bottom of the minute book; (3) get the detectable 2.0mL of embodiment 1, add urine specimen or standard items reagent 2.0mL, mixing left standstill 2 minutes, in 30 ℃ of temperature, wavelength 660nm place colorimetric, write down absorbance A 2; (4) result calculates,
On typical curve, try to achieve corresponding indigo salidroside content.
Concrete experimental result:
Embodiment 9: test urine indigo glucoside
(1) take by weighing indigo glucoside standard items 2.0ug with electronic balance, be dissolved in the 10ml deionized water, be made into 200mg/L concentration, doubling dilution becomes 100mg/L, 50mg/L, 25mg/L, 12.5mg/L successively; Measure respectively
Draw typical curve with coordinate paper; (2) get the detectable 3.5mL of embodiment 7, in 35 ℃ of temperature, wavelength 660nm place colorimetric, absorbance A 1 at the bottom of the minute book; (3) get the detectable 2.0mL of embodiment 7, add urine specimen or standard items reagent 2.0mL, mixing left standstill 2 minutes, in 35 ℃ of temperature, wavelength 660nm place colorimetric, write down absorbance A 2; (4) result calculates,
On typical curve, try to achieve corresponding indigo salidroside content.
Embodiment 10: test urine indigo glucoside
A, preparation indigo glucoside titer 200mg/L, 100mg/L, 50mg/L, 25mg/L, 12.5mg/L; Measure respectively
Draw typical curve;
B, get embodiment 2 detectable 4mL, in 28 ℃ of temperature, wavelength 660nm place colorimetric, absorbance A 1 at the bottom of the minute book;
C, get embodiment 2 detectable 2.5mL, add urine specimen 2.5mL, mixing left standstill 4 minutes, in 25 ℃ of temperature, wavelength 660nm place colorimetric, write down absorbance A 2;
D, result calculate,
On typical curve, try to achieve corresponding indigo salidroside content.
Claims (9)
1. detectable composition is characterized in that this detectable composition is made of following component:
Concentrated hydrochloric acid: 50-120 parts by volume glacial acetic acid: 0-20 parts by volume
Ferric trichloride: 0.5-4 weight portion sodium azide: 0.05-0.2 weight portion
Water: 10-30 parts by volume;
Wherein, the unit of described parts by volume/weight portion pass is the relation of ml/g.
2. detectable composition as claimed in claim 1 is characterized in that this detectable composition is made of following component:
Concentrated hydrochloric acid: 70-100 parts by volume glacial acetic acid: 0-10 parts by volume
Ferric trichloride: 0.5-2 weight portion sodium azide: 0.1 weight portion
Water: 15 parts by volume;
Wherein, the unit of described parts by volume/weight portion pass is the relation of ml/g.
3. detectable composition as claimed in claim 1 is characterized in that this detectable composition is made of following component:
Concentrated hydrochloric acid: 60 parts by volume glacial acetic acid: 15 parts by volume
Ferric trichloride: 0.5 weight portion sodium azide: 0.15 weight portion
Water: 25 parts by volume;
Wherein, the unit of described parts by volume/weight portion pass is the relation of ml/g.
4. detectable composition as claimed in claim 1 is characterized in that this detectable composition is made of following component:
Concentrated hydrochloric acid: 110 parts by volume glacial acetic acid: 5 parts by volume
Ferric trichloride: 3 weight portion sodium azide: 0.1 weight portion
Water: 20 parts by volume;
Wherein, the unit of described parts by volume/weight portion pass is the relation of ml/g.
5. detectable composition as claimed in claim 1 is characterized in that this detectable composition is made of following component:
Concentrated hydrochloric acid: 100 parts by volume, ferric trichloride: 0.8 weight portion,
Sodium azide: 0.1 weight parts water: 15 parts by volume;
Wherein, the unit of described parts by volume/weight portion pass is the relation of ml/g.
6. detectable composition as claimed in claim 1 is characterized in that this detectable composition is made of following component:
Concentrated hydrochloric acid: 80 parts by volume ferric trichlorides: 1 weight portion
Sodium azide: 0.1 weight parts water: 20 parts by volume;
Wherein, the unit of described parts by volume/weight portion pass is the relation of ml/g.
7. as the application in the reagent of the arbitrary described detectable composition of claim 1-6 indigo glucoside in preparation detection urine.
8. a method of testing of urinating the indigo glucoside is characterized in that measuring urine indigo glucoside with absorbance method, and this method comprises the steps: A. preparation indigo glucoside titer 200mg/L, 100mg/L, 50mg/L, 25mg/L, 12.5mg/L; Measure Δ A respectively
Mark, draw typical curve; B. get detectable 2-5mL, temperature 25-37 ℃, wavelength 660nm place colorimetric, absorbance A 1 at the bottom of the minute book; C. get detectable 1.0-3.0mL, add the brilliant reagent of urine specimen or standard in proportion at 1: 1, mixing left standstill 2-5 minute, temperature 25-37 ℃, wavelength 660nm place colorimetric, and record absorbance A 2; D. the result calculates, Δ A=A2-A1; On typical curve, try to achieve corresponding indigo salidroside content;
Wherein used detectable is made by 50-120 parts by volume concentrated hydrochloric acid, 0-20 parts by volume glacial acetic acid, 0.5-4 weight portion ferric trichloride, 0.05-0.2 weight portion sodium azide and 10-30 parts by volume water among step B, the C;
Wherein, the unit of described parts by volume/weight portion pass is the relation of ml/g.
9. the method for testing of urine indigo glucoside as claimed in claim 8 is characterized in that in this method 25 ℃ of temperature of reaction, 2 minutes reaction time.
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| CN101183054B (en) * | 2007-12-14 | 2010-05-26 | 暨南大学 | Urine Disposal Methods |
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|---|---|---|---|---|
| US5965114A (en) * | 1996-11-20 | 1999-10-12 | Wella Ag | Dye-containing mass, composition containing it and method for dyeing keratin fibers, especially human hair |
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