CN101049342B - Extraction method of effective part of walnut and anti-AIDS application - Google Patents

Abstract

The invention provides a method for extracting the effective part of the walnut and a new application of anti-AIDS. The extraction method is as follows: Weigh the raw medicinal material walnut flakes or powder, add water, soak in water for 24 hours at 25-30°C, then use the percolation method to percolate until colorless, rotate and recover the percolation liquid, and obtain the walnut water extract dry powder A. Take walnut water extract dry powder A, add water to dissolve, then apply macroporous adsorption resin HPD-450 to carry out column chromatography, carry out elution with water, 30% ethanol, 50% ethanol, 75% ethanol, 95% ethanol respectively, eluent After rotary recovery and drying, 5 kinds of powders of AA, AB, AC, AD and AE were obtained respectively, among which AB and AE were the effective fractions. The experimental research of the present invention shows that the walnut and its effective parts have good anti-HIV effect and can be used to prepare anti-AIDS medicine.

Description

Extraction method of effective part of walnut and anti-AIDS application

technical field

The invention relates to a method for extracting effective parts of walnut and a new medical application for anti-AIDS.

Background technique

AIDS (AIDS) full name is acquired immunodeficiency syndrome (acquired immunodeficiency syndrome). The pathogen is HIV (human immunodeficiency virus, HIV), the human immunodeficiency virus. The outer layer of the HIV virion is made of fat, known as the viral envelope. There are 72 small lily-like suckers distributed on the surface of each viral outer membrane, and the flowers are scattered on the outer membrane facing outward.

The bulk of the sucker is the gp120 adhesion protein, which is responsible for attaching the virus to the host cell surface. The handle of the sucker is a gp41 fusion protein, which is responsible for fusion and perforation of the host cell membrane. After the virus enters the human body, once it encounters cells with CD4, CXCR4 and CCR5 molecules on the surface, the sucker immediately adsorbs to these molecules, and the fusion protein fuses and perforates the cell membrane, and then releases all the substances in the virus to the host cell , leaving the envelope outside the cell. Immediately after the genetic material enters the cell, HIV reverse transcriptase converts the viral RNA into DNA, which is compatible with human genetic material. This reverse-transcribed DNA is transported into the nucleus and then ligated into human lymphocyte DNA by HIV integrase. Once integrated, these HIV DNAs become pro-AIDS viruses.

There are three enzymes necessary for viral replication in the HIV-1 inner shell, which are called reverse transcriptase, integrase and protease. When the human body is in a healthy state, it has a good defense ability against various pathogens, and the immune system prevents people from getting sick. Once infected with HIV, the immune system is attacked by HIV, especially CD4 lymphocytes die in large numbers, and the human body loses its defense ability, allowing viruses and various pathogens to do whatever they want and cause damage to the human body. Some abnormal cells in the body, such as mutated cancer cells, will also take advantage of the opportunity to grow and multiply at this time, causing people to produce various tumors. At the same time, other protozoa, molds, viruses, and bacteria will also attack the human body and destroy the immune function of the human body. People may suffer from various diseases at the same time, making it difficult for doctors to treat them.

HIV is a highly mutated virus, which is very prone to mutations, so there are many mutant strains of HIV virus. The development of vaccines is limited not only by mutant strains of the virus, but also by the diversity of the population. Different people have different genetic structures and different HIV exposure routes. Theoretically, a vaccine can protect against infection of at least one HIV strain, but in fact no satisfactory vaccine has come out so far. Especially in recent years, it has been found that the speed of HIV mutation is related to the magnitude of external stimuli received.

Due to technical reasons, it is currently impossible for humans to mount an immune response against all genes of the virus, so it is impossible to manufacture a vaccine against the whole gene. If an immune response is carried out against a certain fragment of the gene, not only will it fail to produce the ideal immune effect, it may stimulate the virus to mutate and produce new virus mutants, which means that the speed of vaccine development is not as fast as the speed of virus mutation, which is exactly The difficulty of vaccine research.

Due to the slow progress in vaccine research, people are more hopeful that anti-HIV drugs will play their due role. At present, the mainstream method for the treatment of AIDS at home and abroad is still the high-efficiency combination of antiretroviral drugs (HAART) therapy. The drugs used mainly include the following: zidovudine (AZT), didanosine , ddI, Huitozi), zalcitabine (zalcitabine, ddC), stavudine (stavudine, d4T, Zeret), lamivudine (lamivudine, 3TC), saquinavir (saquinavir, Invirase ), ritonavir (ritonavir, Norvir Nuowei), indinavir (indinavir, Crixivan), nevirapine (nevirapine, Viramune, Wei Leming), nelfinavir (nelfinavir, Viracept, Jiaxiban), Lavudine (delavirdine, DLV), efavirenz (Efavirenz, DMD2266, Shi Duo Ning), enfuvirtide (enfurvitide, T-20), sifuvirtide.

Although HAART therapy can greatly reduce the viral load, it cannot completely eliminate the virus. The virus hidden in deep places such as lymph nodes, organs, bones, skin, muscles, etc. is still unable to be removed. After stopping the drug, the disease will rebound significantly. Regression of treatment or failure of previous efforts. Drugs have many adverse reactions and are prone to drug resistance. Most patients have side effects such as nausea, vomiting, lack of appetite, loss of appetite, decreased liver and kidney function, fat loss, abnormal blood phase indicators, and bone marrow suppression after taking the drug. The long-term application of this method in clinical practice. Due to the limitations, drug resistance and adverse reactions of HAART therapy, people turn to traditional Chinese medicine to treat AIDS, especially to screen out the active ingredients or effective parts of anti-HIV from natural medicines or medicinal plants, which have become AIDS drugs. important area of research.

According to literature records, walnut is warm in nature and sweet in taste, which can cure scrofula and scabies, kill insects and detoxify, improve symptoms of tumors, increase appetite, relieve pain and nourish blood. External washing cures itching all over the body.

At present, there is no relevant report on anti-AIDS of walnut and its effective parts.

Contents of the invention

The purpose of the present invention is to provide a method for extracting effective parts of walnut.

Another object of the present invention is to provide a new application of walnut and its effective parts against AIDS.

The extraction method of effective parts AB and AE of walnut is as follows:

Weigh the native medicinal walnut flakes or powder, add water, soak in water for 24 hours at 25-30°C, then use the percolation method to percolate until it is colorless, rotate and recover the percolation liquid, and obtain the walnut water extract dry powder A, take the walnut Water extract dry powder A, add water to dissolve, then perform column chromatography on macroporous adsorption resin HPD-450, elute with water, 30% ethanol, 50% ethanol, 75% ethanol and 95% ethanol respectively, and the eluent is rotated After recovery and drying, 5 powders of AA, AB, AC, AD, and AE were obtained respectively, wherein AB and AE were effective parts.

The walnut described in the present invention is the twig, bark, root bark, leaf, flower, kernel, the exocarp of immature fruit, the endocarp of ripe fruit, the woody septum of fruit stone of Juglans regia L. Juglans cathayensis Dode.

The above-mentioned eluent is not limited to ethanol, and other organic reagents, such as methanol, petroleum ether, dichloromethane or chloroform, can also be used.

Through silica gel thin layer detection, AB has dark blue, light brown, light red, light blue fluorescent spots at 254nm, and AE has light blue fluorescent spots.

The effective fractions (AB and AE) of walnut described in the present invention can be prepared into conventional pharmaceutical dosage forms, such as oral dosage forms (capsules, oral liquids, granules, tablets) and injections, as pharmaceutical active ingredients.

The experimental research of the present invention shows that the walnut water extract and the effective fractions (AB and AE) have good anti-HIV effect and can be used to prepare anti-AIDS medicaments. The obvious difference from the existing antiretroviral drugs is that the target of the effective part AB is the integrase of HIV-1 virus, and the target of the effective part AE is the cell fusion protein gp41 of HIV-1 virus.

Since walnut is a natural plant with low toxicity and side effects, it has more advantages than artificially synthesized reverse transcriptase inhibitors, so it has a wide application prospect.

Description of drawings

Figure 1: Photomicrograph of a control group of cells.

Figure 2: Photomicrograph of the virus control group.

Figure 3: Photomicrograph of walnut aqueous extract.

Figure 4: Photomicrograph of positive control drug AZT group.

Figure 5: Photomicrographs of active sites AB.

Figure 6: Photomicrographs of active sites AE.

Figure 7: A diagram of the target points of the effective site AB.

Figure 8: AE action target map of the effective site.

Detailed ways

In the following, the anti-HIV effect of the present invention will be further described through test examples.

Test Example 1: Anti-HIV-1IIIB virus effect of walnut water extract and effective parts

(1) Material

Cells, reagents and instruments: MT4 cells and HIV IIIB virus were cultured by our laboratory. RPMI1640 medium, premium grade fetal bovine serum (GIBCO, USA), positive drug AZT is a clinical oral preparation, purchased from GlaxoSmithKline. Ordinary inverted microscope (Chongqing Optical Instrument Factory), desktop high-speed refrigerated centrifuge (German HERAEUS company), phase contrast inverted microscope camera system (Japan OLYMPUS company), biological safety cabinet (US BAKER company).

(2) Test method

Take logarithmic growth of _MT4 cells at 80-1 million/mL and add them to a 75cm ² (250mL) filter cap cell culture flask, then add 30mL RPMI 1640 full medium (10% fetal bovine serum, 1% penicillin, streptomycin, 1% glutamine), culture in a 5% _CO2 incubator at 37°C, change the medium every 3 days, and continue to cultivate, so that the cells can always maintain a good growth state. The whole process of changing the medium must be in a biological safety cabinet in.

The experiment was divided into administration group, cytotoxicity control, virus control (HIV IIIB), positive drug control (AZT) and normal cell control group. The total number of _MT4 cells 24 hours after changing the medium is 2.5 million. Dilute to 10mL with RPMI 1640 full medium for later use. During the experiment, first divide the 96-well plate into groups, each drug has 1 group, each group has 6 concentrations, and each dose 3 duplicate wells, first add 100 μL RPMI 1640 full medium to each well, then add the above prepared drugs to each group according to the doubling dilution ratio, and then add 100 μL cells (250,000/mL) to the cytotoxic control group and normal cells respectively For the control group, mix 200 viruses (HIVIIIB) with half the tissue culture infectious dose (TCID50) and the remaining cells (MT ₄ ), put them in a 5% CO2 incubator and incubate at 37°C for 30 minutes, and take the pre-warmed 100 μL (250,000/mL) of virus cell suspension was added to the corresponding wells of the 96-well plates of each drug administration group and virus control group, placed in a 5% CO2 incubator at 37°C for 6 days, and used on the 6th day. A phase contrast inverted microscope camera system or an inverted microscope equipped with a digital camera will take pictures of the results, and then use the international routine observation method of cytopathic effect (CPE, cytopathic effect) combined with formula calculation to judge the results.

(3) Statistical method

Statistics were carried out according to the BLISS method, and the inhibition percentage was calculated according to the following formula according to the image:

$\mathrm{<span\; class="notranslate">\; IR</span>}<span\; class="notranslate">\; =</span><span\; class="notranslate">\; (</span>\mathrm{<span\; class="notranslate">\; 1</span>}<span\; class="notranslate">\; -</span>\frac{\mathrm{<span\; class="notranslate">\; CC</span>}<span\; class="notranslate">\; -</span>\mathrm{<span\; class="notranslate">\; TG</span>}}{\mathrm{<span\; class="notranslate">\; CC</span>}<span\; class="notranslate">\; \&CenterDot;</span>\mathrm{<span\; class="notranslate">\; VC</span>}}<span\; class="notranslate">\; )</span><span\; class="notranslate">\; \&times;</span>\mathrm{<span\; class="notranslate">\; 100</span>}<span\; class="notranslate">\; \%</span>$

Among them, IR is the inhibition rate, CC is the cell control, VC is the virus control, TG is the experimental group, and 1 is 100% survival rate. When the growth of the cell control is normal, the number of viable cells in CC can be set as 100%, and the formula is 1, then TG and VC are the corresponding decimals below 1. According to the image results, the average number of viable cells in VC is estimated to be about 30% of CC, AZT is about 90%, walnut water extract is about 90%, walnut effective part AB is about 93%, and AE is about 95%. The AZT inhibition rate is calculated as follows, and the rest are deduced by analogy:

$\mathrm{<span\; class="notranslate">\; IR</span>}<span\; class="notranslate">\; =</span><span\; class="notranslate">\; (</span>\mathrm{<span\; class="notranslate">\; 1</span>}<span\; class="notranslate">\; -</span>\frac{\mathrm{<span\; class="notranslate">\; 1</span>}<span\; class="notranslate">\; -</span>\mathrm{<span\; class="notranslate">\; 0.9</span>}}{\mathrm{<span\; class="notranslate">\; 1</span>}<span\; class="notranslate">\; -</span>\mathrm{<span\; class="notranslate">\; 0.3</span>}}<span\; class="notranslate">\; )</span><span\; class="notranslate">\; \&times;</span>\mathrm{<span\; class="notranslate">\; 100</span>}<span\; class="notranslate">\; \%</span>$

$<span\; class="notranslate">\; =</span><span\; class="notranslate">\; (</span>\mathrm{<span\; class="notranslate">\; 1</span>}<span\; class="notranslate">\; -</span>\mathrm{<span\; class="notranslate">\; 0.143</span>}<span\; class="notranslate">\; )</span><span\; class="notranslate">\; \&times;</span>\mathrm{<span\; class="notranslate">\; 100</span>}<span\; class="notranslate">\; \%</span><span\; class="notranslate">\; =</span>\mathrm{<span\; class="notranslate">\; 85.7</span>}<span\; class="notranslate">\; \%</span>$

(4) Results

Table 1 shows the anti-HIV virus results of walnut water extract and effective parts. It can be seen from Table 1 that the cells in the cell control group grew well, the cell survival rate was basically 100%, and there was no cell death. In the virus control group, only 30% of the cells survived, and the remaining 70% of the cells all died, and a large number of syncytia formed in the surviving cells, and the cells showed a tendency of gradual death. There is a significant difference between the two groups, statistically P<0.001, indicating that the virus is highly virulent.

The antiviral effect of walnut water extract is better, the number of living cells accounts for about 90%, and the number of dead cells accounts for about 10%. The cell growth state is much better than that of the virus control group, indicating that the water extract has a good anti-HIV effect. The virus inhibition rate is about 85.7%, equal to that of the positive control drug AZT group, and has obvious statistical significance. IC ₅₀ (half inhibitory concentration) is 31.26 μg/ml, although it is larger than the concentration of AZT, but TC ₅₀ (half toxic concentration) is 425.6 μg/ml, which is many times higher than 29.2 of AZT, indicating that the toxicity of walnut is higher than that of AZT. AZT is low.

The effective part AB of walnut has a good antiviral effect, the number of living cells accounts for about 93%, and the number of dead cells accounts for about 7%. The inhibitory effect is better than that of AZT, and the statistical significance is obvious. The IC ₅₀ is 20.76μg/ml, the TC ₅₀ is 1661.2μg/ml, and the TI (therapeutic index) is 80.02, which is higher than the therapeutic index of the water extract and AZT, showing the characteristics of high efficiency and low toxicity of the effective part of AB.

The effective part AE of walnut has a very good antiviral effect, the number of living cells accounts for about 95%, and the number of dead cells accounts for about 5%. The effective part AE has a stronger anti-HIV effect than the effective part AB, and the inhibition rate against HIV is 92.9%. , statistically significant. IC ₅₀ is 4.15μg/ml, close to the concentration of AZT, TC ₅₀ is 1245.87μg/ml, TI (therapeutic index) is 300, much better antiviral effect than AZT.

According to the second draft of "Technical Guidelines for Non-clinical Pharmacodynamics Research of Anti-HIV Drugs" provided by the National Center for Drug Evaluation on November 23, 2005, drugs with a therapeutic index exceeding 10 are considered to have research value. It is not difficult to find that the aqueous extract of walnut, effective parts AB and AE of walnut described in the present invention all have research and development value.

Table 1 Anti-HIV virus results of walnut and effective parts

drug Dose/mg.mL^-1 Number of Viable Cells (%) Number of dead cells (%) Inhibition rate(%) IC₅₀/μg.mL^-1 TC₅₀/μg.mL^-1 TITC₅₀/IC₅₀ Statistics (P) cell control 100 0 Virus control 30 70 ＜0.001 AZT 0.0205 90 10 85.7 1.6 29.2 18.25 ＜0.001 walnut water extract 3.53 90 10 85.7 31.26 425.6 13.61 ＜0.001 Effective parts of walnut AB 1.0 93 7 90.0 20.76 1661.2 80.02 <0.001 Effective parts of walnut AE 0.0938 95 5 92.9 4.1529 1245.87 300 <0.001

Goodbye photomicrographs 1-6 of walnut aqueous extract and effective parts.

It can be seen from Figure 1 that the growth state of MT4 cells in the cell control group (see Figures a and b) is good, the cells grow in clusters and sheets, each independent but connected with each other through cilia, the cell membrane is round and smooth, and the cytoplasm is crystal clear and full of luster , the number of cells significantly increased compared with the virus control group, and the cell growth was normal.

It can be seen from Figure 2 that the growth state of the cells in the virus control group (see c and d) was poor, a large number of cells died, the cell membrane shrank, the cytoplasm was shriveled, and the number of cells was significantly reduced, and a large number of syncytia appeared in the surviving cells ( syncytia), the color of the cells gradually blackened and died, and the dead cells fused into clusters, which were significantly different from the cell control group. It shows that the virus is very virulent.

It can be seen from Figure 3 that the growth state of the cells in the walnut water extraction group (see e and f) is significantly better than that of the virus control group, which is similar to the cell control group and the positive control drug AZT group. Connected into sheets, no syncytia was seen, the cells were bright in color, the cell membrane was smooth and shiny, the cytoplasm was crystal clear and full, the number of cells and the quality of life were significantly increased compared with the virus control group, indicating that walnut has a strong anti-HIV effect and inhibits HIV infection. The rate was 85.7%, close to or equivalent to the antiviral effect of the AZT group.

It can be seen from Figure 4 that the cells in the positive control drug AZT group (see graphs g and h) were in good growth state, similar to but different from the cell control group. Syncytia was formed, the cells were bright in color, the cell membrane was round, smooth and shiny, the cytoplasm was crystal clear and full, and the number of cells was significantly increased compared with the virus control group, which indicated that AZT had a good antiviral effect, and the inhibition rate of HIV was 85.7%. This experiment proves that AZT does have a good antiviral effect, which is similar to the clinical drug results, indicating that our experimental results have a high degree of credibility.

It can be seen from Figure 5 that the growth state of the cells in the effective part AB of the walnut (see Figures i and j) is also significantly stronger than that of the HIV control group, and even better than that of the AZT group. The cells are connected by cilia and grow in clusters and sheets. , no syncytia was seen, the cells were bright in color, the cell membrane was smooth and shiny, the cytoplasm was crystal clear and full, the number of cells and the quality of life were significantly increased compared with the virus control group, and the inhibition rate of HIV was about 90%, which was higher than that of AZT. The antiviral effect shows that the effective part AB of walnut has a strong antiviral effect.

As can be seen from Fig. 6, the cell growth state of effective parts AE of walnut (see k, l two figures) is better than water extract of walnut, effective parts AB of walnut, AZT group. Cell growth and cell number are much better than the virus control group. No syncytia was seen, the cells were bright in color, the cell membrane was round, smooth and shiny, and the cytoplasm was crystal clear and plump, basically close to the normal cell control group. The effective part AE of walnut is also a very good anti-HIV effective part, and the inhibitory rate to HIV is 92.9%, surpassing the antiviral effect of walnut water extract and effective part AB of walnut, and better than the antiviral effect of AZT.

Test Example 2: Research on anti-HIV targets of walnut and its effective parts

(1) Method

3000分子对接仪对胡桃的有效部位分别进行了作用靶点的研究。首先打开电脑，启动3000控制软件，调用偶联程序，利用偶联试剂把抗HIV-1药物的靶点蛋白(gp120，gp41，p24，integrase，RTaseetc.)偶联在芯片表面，待用。use

The 3000 Molecular Docking Instrument has carried out the research on the effective parts of walnut respectively. First turn on the computer, start 3000 control software, call the coupling program, use the coupling reagent to couple the target protein of the anti-HIV-1 drug (gp120, gp41, p24, integrase, RTaseetc.) on the surface of the chip, ready for use.

Then insert the chip coupled with the target protein into the chip chamber, and at the same time put different types of drugs with different concentrations into the designated position of the instrument, control the equipment to collect samples at the corresponding position for measurement, and observe the drug and target in real time through the sensorgram Interconnection between protein molecules.

3000分子对接仪除了可以判断药物的作用靶点以外，还可以直接判断药物的药理作用及药物的有效部位所在。Judging whether the drug has an effect on the target point and the degree of drug effect according to the peak value of the image, since the drug can only play an effect when it is combined with the target point, the stronger the combination degree of the drug and the target point, the stronger the effect of the drug on the target point. The stronger the degree, the higher the peak of the image, and RU (activity unit) is usually used to represent the height of the peak. The higher the peak, the stronger the inhibitory effect of the drug on the target. And because the target protein is the direct action site of the drug, that is, the specific site where the drug exerts its action, the research on the target point can reflect the action principle of the drug. If the drug does not react with the target protein on the chip, there will be no peaks on the picture, indicating that the experimental target protein of the drug is selected incorrectly, or the drug has no effect on the target, and you can try again later. use

The 3000 Molecular Docking Instrument can not only determine the target of the drug, but also directly determine the pharmacological effect of the drug and the location of the effective part of the drug.

(2) Results

Figure 7 is a 30% ethanol extract (AB) action target diagram, the target protein is HIV-1 integrase, when the concentration of the extract is 20 μg/ml, the binding degree with the target is 316.8RU, the binding degree is relatively powerful. The stronger the binding degree, the higher the antiviral activity. This figure shows that the 30% ethanol extract (AB) has a strong inhibitory effect on HIV-1 integrase.

Figure 8 is a 95% ethanol extract (AE) action target diagram, the target protein is the gp41 fusion protein on the outer membrane of HIV, when the concentration of the extract is 125 μg/ml, the degree of binding to the target is 501RU, the binding The degree is strong. As mentioned above, the stronger the binding degree is, the higher the antiviral activity is. This figure shows that 95% ethanol extract (AE) has a strong inhibitory effect on HIV-1 gp41 fusion protein.

Since AZT is the first choice drug for anti-HIV virus and the most commonly used drug for clinical treatment of AIDS, it is more convincing to use it as a positive control drug. According to the research results of the targets, the effective parts AB and AE of walnut are HIV integrase inhibitors and GP41 cell fusion inhibitors respectively.

The following examples further illustrate the preparation method of the present invention.

Embodiment 1: Preparation 1 of walnut aqueous extract

Weigh 5 kg of native medicinal walnut flakes or powder, add 5 times the amount of water, soak in water for 24 hours at 28°C, then use the percolation method to percolate until colorless, rotate and recover the percolation liquid, and obtain the walnut water extract Dry Powder A (479.93 g).

Embodiment 2: the preparation 2 of walnut aqueous extract

Weigh 5 kg of native medicinal walnut flakes or powder, add 5 times the amount of water, decoct under vacuum at 55°C for 3 times, each time for 3 hours, filter, combine the filtrates for 3 times, concentrate and dry into water extract dry powder B (479.93 g), dry and store for future use.

Embodiment 3: Preparation of effective parts of walnut

Take 8 grams of walnut water extract dry powder A, add 10ml of water to dissolve, then apply macroporous adsorption resin HPD-450 for column chromatography, and elute with water, 30% ethanol, 50% ethanol, 75% ethanol and 95% ethanol respectively , the eluent was recovered and dried by rotation, and 5 kinds of powders of AA (4.32g), AB (1.76g), AC (0.88g), AD (0.096g) and AE (0.072g) were obtained respectively, among which AB and AE were effective parts.

Embodiment 4: the preparation of oral dosage form

Get the walnut water extract dry powder A prepared in Example 1, add starch, granulate, granulate, and make granules.

Take the walnut water extract dry powder B prepared in Example 2, add starch, granulate, granulate, pack into capsules or compress into tablets to obtain capsules or tablets.

Take the walnut effective parts AB and AE powders prepared in Example 3, add starch respectively, granulate, granulate, compress into tablets or pack into capsules to obtain tablets or capsules.

Embodiment 5: the preparation of injection

The walnut effective fractions AB and AE powders prepared in Example 3 were subjected to Sephadex LH20 column chromatography to obtain more pure two effective fractions. It is further refined and purified, and added with a pH regulator and an osmotic pressure regulator to prepare an injection.

Claims (5)

1. Use of walnut as the only active ingredient in the preparation of anti-AIDS medicaments. 2. purposes according to claim 1, wherein said walnut is the exocarp of Juglandaceae plant Juglansregia L., bark, root, leaf, flower, kernel, immature fruit, the endocarp of ripe fruit, Woody septum of the core or Juglans cathayensis Dode. 3. The effective parts AB and AE of walnut with anti-AIDS effect are characterized in that they are prepared by the following method: Weigh the original medicinal material walnut flakes or powder, add water, soak in water for 24 hours at 25-30°C, and then use Percolation method percolation until colorless, rotary recovery of percolation liquid, to obtain walnut water extract dry powder A, take walnut water extract dry powder A, add water to dissolve, then perform column chromatography on macroporous adsorption resin HPD-450, separate water , 30% ethanol, 50% ethanol, 75% ethanol and 95% ethanol for elution, and the eluate is recovered and dried by rotation to obtain 5 kinds of powders of AA, AB, AC, AD, and AE, of which AB and AE are the effective parts . 4. Use of the effective fractions AB and AE of walnut according to claim 3 in the preparation of anti-AIDS medicaments. 5. The method for preparing the effective parts AB and AE of walnut with anti-AIDS effect, which is characterized in that the original medicinal material walnut flakes or powder is weighed, added with water, soaked in water for 24 hours at 25-30°C, and then percolated by percolation method until colorless, rotate and recover the percolation liquid to obtain walnut aqueous extract dry powder A, take walnut aqueous extract dry powder A, add water to dissolve, then apply macroporous adsorption resin HPD-450 for column chromatography, use water, 30% ethanol, and 50% ethanol, 75% ethanol and 95% ethanol were eluted, and the eluate was recovered and dried by rotation to obtain 5 kinds of powders of AA, AB, AC, AD, and AE respectively, wherein AB and AE were effective parts.

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