CN101049328A - Technique for producing extractive of olive growing leaves - Google Patents
Technique for producing extractive of olive growing leaves Download PDFInfo
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- CN101049328A CN101049328A CN 200710107642 CN200710107642A CN101049328A CN 101049328 A CN101049328 A CN 101049328A CN 200710107642 CN200710107642 CN 200710107642 CN 200710107642 A CN200710107642 A CN 200710107642A CN 101049328 A CN101049328 A CN 101049328A
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- 240000007817 Olea europaea Species 0.000 title claims abstract description 29
- 238000000034 method Methods 0.000 title claims description 31
- 239000000284 extract Substances 0.000 claims abstract description 26
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 25
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims abstract description 24
- 238000000926 separation method Methods 0.000 claims abstract description 18
- 238000001291 vacuum drying Methods 0.000 claims abstract description 11
- 238000001914 filtration Methods 0.000 claims abstract description 10
- 238000005342 ion exchange Methods 0.000 claims abstract description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 10
- 238000010298 pulverizing process Methods 0.000 claims abstract description 9
- 239000012528 membrane Substances 0.000 claims abstract description 6
- 235000002725 Olea europaea Nutrition 0.000 claims abstract description 4
- 238000001035 drying Methods 0.000 claims abstract description 4
- -1 filtering Substances 0.000 claims abstract description 4
- 238000010992 reflux Methods 0.000 claims abstract description 4
- 238000000605 extraction Methods 0.000 claims description 16
- 239000000287 crude extract Substances 0.000 claims description 15
- 229940114496 olive leaf extract Drugs 0.000 claims description 8
- 229930003944 flavone Natural products 0.000 claims description 7
- 235000011949 flavones Nutrition 0.000 claims description 7
- 238000000746 purification Methods 0.000 claims description 7
- 238000002137 ultrasound extraction Methods 0.000 claims description 5
- 241000207834 Oleaceae Species 0.000 claims description 4
- 150000002213 flavones Chemical class 0.000 claims description 4
- 239000004615 ingredient Substances 0.000 claims description 4
- 150000002989 phenols Chemical class 0.000 claims description 4
- 150000008442 polyphenolic compounds Chemical class 0.000 claims description 4
- 235000013824 polyphenols Nutrition 0.000 claims description 4
- GAMYVSCDDLXAQW-AOIWZFSPSA-N Thermopsosid Natural products O(C)c1c(O)ccc(C=2Oc3c(c(O)cc(O[C@H]4[C@H](O)[C@@H](O)[C@H](O)[C@H](CO)O4)c3)C(=O)C=2)c1 GAMYVSCDDLXAQW-AOIWZFSPSA-N 0.000 claims description 3
- 238000007598 dipping method Methods 0.000 claims description 3
- 150000002212 flavone derivatives Chemical class 0.000 claims description 3
- 238000005325 percolation Methods 0.000 claims description 3
- VHBFFQKBGNRLFZ-UHFFFAOYSA-N vitamin p Natural products O1C2=CC=CC=C2C(=O)C=C1C1=CC=CC=C1 VHBFFQKBGNRLFZ-UHFFFAOYSA-N 0.000 claims description 3
- 241000795633 Olea <sea slug> Species 0.000 claims description 2
- 239000004743 Polypropylene Substances 0.000 claims description 2
- 239000002131 composite material Substances 0.000 claims description 2
- 238000002481 ethanol extraction Methods 0.000 claims description 2
- 239000012535 impurity Substances 0.000 claims description 2
- 238000003808 methanol extraction Methods 0.000 claims description 2
- 229920002492 poly(sulfone) Polymers 0.000 claims description 2
- 229920001155 polypropylene Polymers 0.000 claims description 2
- JBQLQIMCKFDOHK-UHFFFAOYSA-N Stephanol Natural products CC(O)C1(O)CCC2(O)C3(O)CC=C4CC(O)CCC4(C)C3C(O)C(O)C12C JBQLQIMCKFDOHK-UHFFFAOYSA-N 0.000 claims 1
- 238000004519 manufacturing process Methods 0.000 abstract description 5
- 238000007873 sieving Methods 0.000 abstract 1
- 239000012141 concentrate Substances 0.000 description 7
- 238000005516 engineering process Methods 0.000 description 4
- 241000196324 Embryophyta Species 0.000 description 3
- 239000008280 blood Substances 0.000 description 3
- 210000004369 blood Anatomy 0.000 description 3
- 239000003814 drug Substances 0.000 description 3
- 235000013399 edible fruits Nutrition 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- 230000003110 anti-inflammatory effect Effects 0.000 description 2
- 230000003064 anti-oxidating effect Effects 0.000 description 2
- 239000000470 constituent Substances 0.000 description 2
- 206010012601 diabetes mellitus Diseases 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 229930003935 flavonoid Natural products 0.000 description 2
- 150000002215 flavonoids Chemical class 0.000 description 2
- 235000017173 flavonoids Nutrition 0.000 description 2
- 230000036039 immunity Effects 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 206010010774 Constipation Diseases 0.000 description 1
- 206010012735 Diarrhoea Diseases 0.000 description 1
- 208000035150 Hypercholesterolemia Diseases 0.000 description 1
- 206010020772 Hypertension Diseases 0.000 description 1
- XQFRJNBWHJMXHO-RRKCRQDMSA-N IDUR Chemical compound C1[C@H](O)[C@@H](CO)O[C@H]1N1C(=O)NC(=O)C(I)=C1 XQFRJNBWHJMXHO-RRKCRQDMSA-N 0.000 description 1
- 206010030113 Oedema Diseases 0.000 description 1
- 208000004880 Polyuria Diseases 0.000 description 1
- 206010057190 Respiratory tract infections Diseases 0.000 description 1
- 230000002159 abnormal effect Effects 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- 238000013019 agitation Methods 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000003288 anthiarrhythmic effect Effects 0.000 description 1
- 230000000843 anti-fungal effect Effects 0.000 description 1
- 230000000840 anti-viral effect Effects 0.000 description 1
- 229940121375 antifungal agent Drugs 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 230000003385 bacteriostatic effect Effects 0.000 description 1
- 230000004888 barrier function Effects 0.000 description 1
- 230000003796 beauty Effects 0.000 description 1
- 235000019658 bitter taste Nutrition 0.000 description 1
- 230000036772 blood pressure Effects 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 210000003169 central nervous system Anatomy 0.000 description 1
- 239000000812 cholinergic antagonist Substances 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 235000015872 dietary supplement Nutrition 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 230000035619 diuresis Effects 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 229910001385 heavy metal Inorganic materials 0.000 description 1
- 210000000936 intestine Anatomy 0.000 description 1
- 238000002386 leaching Methods 0.000 description 1
- 239000002932 luster Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000001471 micro-filtration Methods 0.000 description 1
- 238000001728 nano-filtration Methods 0.000 description 1
- 239000004006 olive oil Substances 0.000 description 1
- 235000008390 olive oil Nutrition 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 230000001603 reducing effect Effects 0.000 description 1
- 230000035943 smell Effects 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 238000000638 solvent extraction Methods 0.000 description 1
- 230000002048 spasmolytic effect Effects 0.000 description 1
- 238000001694 spray drying Methods 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000000108 ultra-filtration Methods 0.000 description 1
- 238000002604 ultrasonography Methods 0.000 description 1
- 208000019206 urinary tract infection Diseases 0.000 description 1
- 230000024883 vasodilation Effects 0.000 description 1
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- Medicines Containing Plant Substances (AREA)
Abstract
A process for preparing the extract of olive leaf includes such steps as low-temp drying in dark condition, pulverizing, extracting in ethanol or methanol by reflux, immersing, seeping, or ultrasonic wave, filtering, vacuum concentrating, vacuum drying to obtain coarse extract, pulverizing, sieving, dissolving in water, filtering, membrane separation, and purifying by ion exchange.
Description
Technical field
The present invention relates to a kind of production technology of olive leaf extract.
Background technology
Fructus oleae europaeae, (Olea europaea L.) claims olive, Europe Fructus Canarii albi, olive again, is Oleaceae (Oleaceae) Olea (Olea L) plant, is that famous and precious evergreen woody oleiferous plants in the world and fruit are used seeds.Fructus oleae europaeae originates in Asia Minor, mainly is distributed in littoral area, Mediterranean, is to concentrate the place of production with Spain, Italy, Portugal and Albania.1964, when the Premier Zhou Enlai visits Albania, recognize after the special efficacy and extensive use of olive oil that decision is introduced and promoted.Introduce a fine variety the Fructus oleae europaeae expert effort of decades through China, introduce a fine variety and obtained success.The Fructus oleae europaeae output of normal region has reached the level in original producton location.According to incompletely statistics, existing about 400,000 mu of the Fructus oleae europaeae area (1,000 ten thousand strain) of China.
Fructus oleae europaeae is the conventional medicament of Mediterranean Region, is used for the treatment of multiple disease with leaf and fruit medicine.The decoct of Folium olive or preserved material have certain curative effect in the medication among the people of mediterranean country Chang Zuowei diabetes.Olive leaf extract is used as dietary supplement with raise immunity in America and Europe.Modern data shows that Fructus oleae europaeae has anti-arrhythmia, antifungal, anti-hypercholesterolemia, blood sugar lowering, blood pressure lowering, antiinflammatory, antioxidation, antiviral, diuresis, the multiple pharmacologically active of central nervous system such as spasmolytic and vasodilation, be used for the treatment of diabetes, hypertension, diarrhoea, edema, the intestines and stomach angor, herpes, respiratory tract infection, constipation, diseases such as urinary tract infection.Separating the chemical constituent that obtains from leaf of Fructus oleae europaeae can be divided into: flavonoid, secoiridoid class, saccharide, organic acid and other chemical compound.Olive leaf extract has many middle functions such as blood sugar lowering, blood fat reducing, bacteriostatic activity, antioxidant activity, anti-inflammatory activity enhance immunity and beauty treatment, and potentiality to be exploited is huge.Modern biotechnology the analysis showed that active substance mainly contains olive polyphenol, flavonoid, secoiridoid class (comprising cleupin) etc. in the leaf of Fructus oleae europaeae, and its antioxidation biology active matter content is higher than Fructus oleae europaeae fruit and stem.
At present domestic have report to extract the processing Fructus Canrii Albi extract with Folium olive and leaf of Fructus oleae europaeae, but adopt mostly water carry or alcohol extraction after direct spray drying composition, cleupin, total phenols and flavones content are low in the product, are starkly lower than external required standard extractive content.The present invention's extraction, separation and purification to the Folium olive effective ingredient on traditional handicraft extraction, isolating basis improves, to improve separation and purification efficient.The present invention adopts reflux, extract,, percolation to extract and ultrasonic extracting method such as auxiliary, and membrane separation technique and ion exchange technique method that vacuum concentration, drying, crude extract take microfiltration, ultrafiltration, nanofiltration to combine are carried out purification; Contrast with traditional leach extraction method, determine best extraction, the scheme of separation and purification.The application of membrane separation technique in extracting effective ingredients in plant obtained very big development in recent years, is a kind of promising new technology.Ultrasonic assisted extraction method is ultrasound wave and conventional solvent extraction method to be combined and a kind of new extracting method that forms.Compare with conventional extraction method have applied widely, that raw material is handled is convenient, extraction efficiency is high, save time, save advantage such as solvent.
Summary of the invention
The object of the present invention is to provide a kind of production technology that yield height and extract active constituent content height and extract mainly contain the olive leaf extract of cleupin, total phenols and three kinds of compositions of flavone of extracting.
Purpose of the present invention realizes by following manner:
1. with leaf of Fructus oleae europaeae low-temperature dark drying, pulverize;
2. the leaf of Fructus oleae europaeae after pulverizing adds 1～20 times of ethanol or methanol extraction;
3. leachate is filtered, vacuum concentration, vacuum drying gets the leaf of Fructus oleae europaeae crude extract;
4. crude extract is pulverized, crossed 20～80 mesh sieves, the after-filtration that is dissolved in water is removed impurity;
5. through membrance separation, the ion exchange separation and purification gets the leaf of Fructus oleae europaeae extract.
Technique for producing extractive of olive growing leaves of the present invention can add the leaf of Fructus oleae europaeae after pulverizing 1～20 times of ethanol or methanol eddy and extract.
Technique for producing extractive of olive growing leaves of the present invention also can add the leaf of Fructus oleae europaeae after pulverizing 1～20 times of ethanol or methanol dipping and extract determining alcohol 30～95%, 25～65 ℃ of extraction temperatures, extraction time 36～144h; Agitation as appropriate is to increase extraction efficiency in the leaching process.
Technique for producing extractive of olive growing leaves of the present invention also can add the leaf of Fructus oleae europaeae after pulverizing 1～20 times of ethanol or methanol percolation and extract.
Technique for producing extractive of olive growing leaves of the present invention also can add 1～20 times of ethanol or methanol ultrasonic extraction, frequency 20～80kHz, time 20～180min, 20～55 ℃ of temperature with the leaf of Fructus oleae europaeae after pulverizing.
The described vacuum concentration temperature of technique for producing extractive of olive growing leaves of the present invention is no more than 55 ℃.
The described vacuum drying temperature of technique for producing extractive of olive growing leaves of the present invention is no more than 55 ℃.
The employed film of technique for producing extractive of olive growing leaves of the present invention sees table 1 for details.The present invention utilizes membrane separation technique, with the diffusion barrier of PSPP, the leaf of Fructus oleae europaeae extracting solution is divided into different fraction by the size of molecular weight, to the part that cleupin content is concentrated carried out concentrating, dry, analyze.
The present invention compared with prior art, it is reasonable to have a process route, the extraction ratio height, good product quality is suitable for advantages such as suitability for industrialized production.Extract mainly contains cleupin, total phenols and three kinds of effective ingredient of flavone.The extraction ratio of its crude extract is 10～25%, and product cleupin content reaches 2～6%, and flavones content reaches 20～30%, and polyphenol content reaches 8～12%.The olive leaf extract technical specification sees Table 2.
The specific embodiment
The following examples can make those skilled in the art more fully understand the present invention, but do not limit the present invention in any way.
Embodiment 1
Get the exsiccant leaf of Fructus oleae europaeae of 100kg, pulverize, add 10 times of 90% alcohol reflux, leachate is filtered, vacuum concentration, vacuum drying concentrates and baking temperature is no more than 55 ℃, gets leaf of Fructus oleae europaeae crude extract 16kg.Crude extract is pulverized, crossed 80 mesh sieves, the after-filtration that is dissolved in water, through membrance separation, the ion exchange separation and purification gets leaf of Fructus oleae europaeae extract 1.8kg.
Embodiment 2
Get the exsiccant leaf of Fructus oleae europaeae of 100kg, pulverizing adds 15 times of 80% methanol eddy and extracts, leachate is filtered, and vacuum concentration, vacuum drying concentrates and baking temperature is no more than 55 ℃, gets leaf of Fructus oleae europaeae crude extract 13kg.Crude extract is pulverized, crossed 60 mesh sieves, the after-filtration that is dissolved in water, through membrance separation, ion exchange separates alcoholization, gets leaf of Fructus oleae europaeae extract 1.5kg.
Embodiment 3
Get the exsiccant leaf of Fructus oleae europaeae of 100kg, pulverize, add 10 times of 85% alcohol dipping and extract 2 times, each 48h, 55 ℃ of extraction temperatures merge lixiviating solution, filter, vacuum concentration, vacuum drying concentrates and baking temperature is no more than 55 ℃, leaf of Fructus oleae europaeae crude extract 19kg.Crude extract is pulverized, crossed 80 mesh sieves, the after-filtration that is dissolved in water, through membrance separation, ion exchange separates alcoholization, gets leaf of Fructus oleae europaeae extract 1.8kg.
Embodiment 4
Get the exsiccant leaf of Fructus oleae europaeae of 100kg, pulverize, add 20 times of 90% ethanol percolate extraction, will carry thing liquid and filter, vacuum concentration, vacuum drying concentrates and baking temperature is no more than 55 ℃, gets leaf of Fructus oleae europaeae and slightly extracts 15kg.Crude extract is pulverized, crossed 80 mesh sieves, the after-filtration that is dissolved in water, through membrance separation, ion exchange separates alcoholization, gets leaf of Fructus oleae europaeae extract 1.7kg.
Embodiment 5
Get the exsiccant leaf of Fructus oleae europaeae of 100kg, pulverize, add 20 times of 95% ethanol ultrasonic extraction, supersonic frequency 20kHz, each ultrasonic 2min, work is 0.2s intermittently, omnidistance time 45min, 45 ℃ of temperature repeat to extract twice, amount to 90min, thing liquid be will carry and vacuum concentration, vacuum drying filtered, concentrate with baking temperature and be no more than 55 ℃, get leaf of Fructus oleae europaeae and slightly extract 25kg.Crude extract is pulverized, crossed 80 mesh sieves, the after-filtration that is dissolved in water, through membrance separation, ion exchange separates alcoholization, gets leaf of Fructus oleae europaeae extract 2.4kg.
Embodiment 6
Get the exsiccant leaf of Fructus oleae europaeae of 100kg, pulverize, add 20 times of 80% methanol ultrasonic extraction, supersonic frequency 40kHz, each ultrasonic 2min, work is 0.2s intermittently, omnidistance time 50min, 45 ℃ of temperature repeat to extract twice, amount to 100min, thing liquid be will carry and vacuum concentration, vacuum drying filtered, concentrate with baking temperature and be no more than 55 ℃, get leaf of Fructus oleae europaeae and slightly extract 22kg.Crude extract is pulverized, crossed 80 mesh sieves, the after-filtration that is dissolved in water, through membrance separation, ion exchange separates alcoholization, gets leaf of Fructus oleae europaeae extract 2.1kg.
This institute of table 1. uses the key property and the manufacturer of film
| Kind | Molecular cut off | Applicable pH | Applicable temperature | Manufacturer |
| Polysulfone membrane (PS) polypropylene screen (PAN) polysulfonamides film (PSA) composite | 2000、4000 10000 70000，140000 500，1000 | 2～12 2～12 2～12 1～13 | ＜50℃ ＜50℃ ＜50℃ 5～60℃ | The Shanghai atomic nucleus is matched general film scientific ﹠ technical corporation |
Table 2. olive leaf extract technical specification
| Project | Technical specification |
| Abnormal smells from the patient | Bitter taste |
| Color and luster | Bottle green is to brown |
| Character | Powdery or paste |
| Moisture content | ≤5％ |
| Ash | ≤5％ |
| Heavy metal | ≤10mg/kg |
| Granularity | 80 orders |
| Total number of bacteria | ≤1000cfu/g |
| Cleupin | ≥2％ |
| Total flavones | ≥20％ |
| Polyphenol | ≥8％ |
Claims (10)
1. technique for producing extractive of olive growing leaves mainly may further comprise the steps:
1. with leaf of Fructus oleae europaeae low-temperature dark drying, pulverize;
2. the leaf of Fructus oleae europaeae after pulverizing is with ethanol or methanol extraction;
3. leachate is filtered, vacuum concentration, vacuum drying gets the leaf of Fructus oleae europaeae crude extract;
4. crude extract is pulverized, sieved, the after-filtration that is dissolved in water is removed impurity;
5. through membrance separation, the ion exchange separation and purification gets the leaf of Fructus oleae europaeae extract;
Olive leaf extract mainly contains cleupin, total phenols and three kinds of effective ingredient of flavone.
2. according to the described technique for producing extractive of olive growing leaves of claim 1, it is characterized in that 2. described ethanol of step or methanol concentration 30～95%.
3. according to the described technique for producing extractive of olive growing leaves of claim 1, it is characterized in that the 2. described pure extracting method of step is a reflux, extract.
4. according to the described technique for producing extractive of olive growing leaves of claim 1, it is characterized in that the 2. described pure extracting method of step extracts 1～20 times of pure consumption, determining alcohol 30～95%, 25～65 ℃ of extraction temperatures, extraction time 36～144h for dipping.
5. according to the described technique for producing extractive of olive growing leaves of claim 1, it is characterized in that the 2. described pure extracting method of step is that percolation extracts.
6. according to the described technique for producing extractive of olive growing leaves of claim 1, it is characterized in that the 2. described pure extracting method of step is a ultrasonic extraction, frequency 20～80kHz, time 20～180min, 20～55 ℃ of temperature.
7. according to the described technique for producing extractive of olive growing leaves of claim 1, it is characterized in that described vacuum concentration temperature is no more than 55 ℃; Described vacuum drying temperature is no more than 55 ℃.
8. according to the described technique for producing extractive of olive growing leaves of claim 1, it is characterized in that the 5. used film of step is polysulfone membrane (PS), polypropylene screen (PAN), polysulfonamides film (PSA) or composite.
9. according to the described technique for producing extractive of olive growing leaves of claim 1, it is characterized in that described olive leaf extract cleupin content 2～6%, flavones content 20～30%, polyphenol content 8～12%.
10. according to the described technique for producing extractive of olive growing leaves of claim 1, it is characterized in that described leaf of Fructus oleae europaeae is all leaf of Fructus oleae europaeae of Olea europaea kind (Olea europaea L) of Oleaceae (Oleaceae) Olea (Olea L).
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| CN 200710107642 CN101049328A (en) | 2007-05-23 | 2007-05-23 | Technique for producing extractive of olive growing leaves |
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| CN 200710107642 CN101049328A (en) | 2007-05-23 | 2007-05-23 | Technique for producing extractive of olive growing leaves |
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| CN101912109A (en) * | 2010-07-09 | 2010-12-15 | 中国林业科学研究院林产化学工业研究所 | A kind of health food with anti-hypoxia function and preparation method thereof |
| CN102224958A (en) * | 2011-05-18 | 2011-10-26 | 福州市鼓楼区大特生物技术有限公司 | Method for extracting green olive juice with ultrasonic at low temperature |
| CN102260588A (en) * | 2011-06-21 | 2011-11-30 | 西北师范大学 | Method for synchronously extracting pomace oil and polyphenol from olive processing waste residue |
| CN101781344B (en) * | 2009-12-13 | 2012-06-27 | 成都普思生物科技有限公司 | Method for preparing olive leaf extract |
| CN102727409A (en) * | 2012-07-04 | 2012-10-17 | 广东雅威生物科技有限公司 | Moisturizing cream containing natural olive oil and derivatives of natural olive oil, and preparation method of moisturizing cream |
| CN103830332A (en) * | 2012-11-20 | 2014-06-04 | 中国科学院兰州化学物理研究所 | Method for extracting anti-diabetic active parts in olive leaf |
| CN104311613A (en) * | 2014-09-30 | 2015-01-28 | 桂林三宝药业有限公司 | Method for extracting oleuropein from olive leaves |
| CN104622971A (en) * | 2013-11-07 | 2015-05-20 | 中国科学院兰州化学物理研究所 | Pharmaceutical composition for resisting diabetes activity and preparation method thereof |
| CN107969552A (en) * | 2017-12-24 | 2018-05-01 | 中国林业科学研究院林产化学工业研究所 | A kind of extractive of olive growing leaves feed addictive and its application |
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| CN101781344B (en) * | 2009-12-13 | 2012-06-27 | 成都普思生物科技有限公司 | Method for preparing olive leaf extract |
| CN101912109A (en) * | 2010-07-09 | 2010-12-15 | 中国林业科学研究院林产化学工业研究所 | A kind of health food with anti-hypoxia function and preparation method thereof |
| CN101912109B (en) * | 2010-07-09 | 2012-12-19 | 中国林业科学研究院林产化学工业研究所 | Health-care food with hypoxia tolerance function and preparation method thereof |
| CN102224958B (en) * | 2011-05-18 | 2013-05-22 | 福州市鼓楼区大特生物技术有限公司 | Method for extracting green olive juice with ultrasonic at low temperature |
| CN102224958A (en) * | 2011-05-18 | 2011-10-26 | 福州市鼓楼区大特生物技术有限公司 | Method for extracting green olive juice with ultrasonic at low temperature |
| CN102260588A (en) * | 2011-06-21 | 2011-11-30 | 西北师范大学 | Method for synchronously extracting pomace oil and polyphenol from olive processing waste residue |
| CN102260588B (en) * | 2011-06-21 | 2013-01-02 | 西北师范大学 | Method for synchronously extracting pomace oil and polyphenol from olive processing waste residue |
| CN102727409B (en) * | 2012-07-04 | 2013-05-29 | 广东雅威生物科技有限公司 | Moisturizing cream containing natural olive oil and derivatives of natural olive oil, and preparation method of moisturizing cream |
| CN102727409A (en) * | 2012-07-04 | 2012-10-17 | 广东雅威生物科技有限公司 | Moisturizing cream containing natural olive oil and derivatives of natural olive oil, and preparation method of moisturizing cream |
| CN103830332A (en) * | 2012-11-20 | 2014-06-04 | 中国科学院兰州化学物理研究所 | Method for extracting anti-diabetic active parts in olive leaf |
| CN104622971A (en) * | 2013-11-07 | 2015-05-20 | 中国科学院兰州化学物理研究所 | Pharmaceutical composition for resisting diabetes activity and preparation method thereof |
| CN104311613A (en) * | 2014-09-30 | 2015-01-28 | 桂林三宝药业有限公司 | Method for extracting oleuropein from olive leaves |
| CN107969552A (en) * | 2017-12-24 | 2018-05-01 | 中国林业科学研究院林产化学工业研究所 | A kind of extractive of olive growing leaves feed addictive and its application |
| CN110215423A (en) * | 2019-06-19 | 2019-09-10 | 珠海远大美业生物科技有限公司 | The anti-ageing composition of reparation containing various plants extract, preparation method and application |
| CN114468095A (en) * | 2022-02-23 | 2022-05-13 | 兰州海关技术中心 | Plant active tea and preparation method thereof |
| CN115444791A (en) * | 2022-08-08 | 2022-12-09 | 何直源 | Olive leaf fermented extract and its preparation method and application |
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