CN100999614B - Haematochrome extracted from fragrans seed peel and its preparation process and application - Google Patents

Abstract

The present invention will disclose the red pigment extracted from the osmanthus osmanthus seed skin, which is prepared by the following method: 1) putting the osmanthus osmanthus seed skin into an acidic polar solvent to extract; 2) filtering the obtained extract; 3. ) filtrate evaporated, the resulting residue is the red pigment extracted from the osmanthus seed husk. The red pigment extracted from the osmanthus osmanthus seed skin of the present invention has good water and alcohol solubility, strong coloring power, is safe, has no toxic and side effects, and has a wide range of uses. It can also be used as a coloring agent for sugar-coated tablets of pharmaceuticals and health products; or as an additive for cosmetics to prepare various cosmetics such as lipstick, blush, etc., and can also be used as a coloring agent for hair dyeing, nail dyeing, and dyeing. In addition, it can also be used as industrial colorants, dyes, biological dyes, and biological reagents.

Description

Red pigment extracted from osmanthus osmanthus seed skin, its preparation method and use

(1) Technical field:

The invention relates to a food pigment, in particular to a red pigment extracted from osmanthus osmanthus seed skin; the invention also relates to a preparation method and application of the red pigment.

(two) background technology:

Food coloring, also known as coloring agent, is a food additive for the purpose of food coloring and improving food color. With the rapid development of the food industry, the demand for food coloring is also increasing. Due to their different sources, food coloring can be divided into two categories: synthetic coloring and natural coloring. As far as the history of food coloring application is concerned, before the middle of the 19th century, relatively crude natural coloring was used as food coloring; Later, due to the advent of synthetic pigments one after another, and because of their bright color, good stability, strong tinting strength, suitable for color matching, easy to dissolve, uniform quality, odorless and tasteless, and low cost, they are widely used in In food, to increase the patterns and varieties of food and improve people's appetite, but with the improvement of people's living standards and the increase of health awareness, higher requirements are put forward for the synthetic pigments used in foods such as beverages, candies, cakes and wines. requirements. The commonly used synthetic pigments in the past have azo group (-N=N-), which is a chromogenic group. The compounds containing this group are all colored substances, but many azo compounds are carcinogens and have Different degrees of chronic toxicity, carcinogenicity and teratogenicity. In recent years, due to the increasing concern about the toxicity of synthetic pigments, the use of synthetic pigments is gradually restricted or even banned in many developed countries abroad, and its use is gradually being phased out in China. The vast majority of natural pigments are safe and non-toxic to eat, natural in color, and many varieties have physiological activities, as well as certain nutritional, medical care and anti-inflammatory effects. Nucleic acid, flavonoid pigments and certain essential elements are not only widely used in various cosmetics, food, beverages and other products to help correct the deviation of color ratio and emphasize the style of different foods, but also can be used in medicine , health care products and other industries, so in the international market, the annual growth rate of natural pigment sales is maintained at about 10%, so domestic units and personnel engaged in the research and development of natural food pigments are also increasing, and the development and utilization of plant natural pigments to replace artificial synthesis Pigment has become a historical necessity. Compared with edible synthetic pigments, edible natural pigments have the following advantages: 1. Most natural pigments are non-toxic and have no side effects, and have high safety; 2. Many natural pigments contain essential nutrients for the human body, or they are vitamins Or substances with vitamin properties, such as riboflavin, β-carotene, etc.; 3. Some natural pigments have certain pharmacological effects, and have preventive and therapeutic effects on certain diseases, such as rutin. The normal resistance of capillaries and the prevention of arteriosclerosis have been used in medicine as auxiliary drugs and nutritional supplements for the treatment of cardiovascular diseases; 4. The coloring tone of natural pigments is more natural and closer to the color of natural substances. However, it has not been found that someone uses the osmanthus seed skin as a raw material to extract the red pigment.

(3) Contents of the invention:

The present invention will disclose the red pigment that has strong tinting strength, thermal stability, oxidation resistance and reducibility, and sugar tolerance and salt tolerance, etc., extracted from osmanthus osmanthus seed skin; Preparation method and use.

Osmanthus fragrans (Osmanthus fragrans), also known as Osmanthus fragrans, is an evergreen tree or shrub of the Osmanthus family, native to southwest my country, mainly distributed in Guangxi, Hunan, Guizhou and Fujian. It has been cultivated in my country for more than 3,000 years. Its leaves are thick green, and it tastes like flowers in four seasons. It is a beautiful tree. In addition to being used in gardens for solitary planting and group planting, it is also an important flower arrangement material because of its rich and unique floral fragrance. Osmanthus fragrans extract can be extracted, which is widely used in food, cosmetics and flavors and fragrances. Osmanthus fragrans seeds are oval, and the seed skin color is purple-black, similar to purple grape skin and black bean skin, and they contain certain red and melanin. In order to effectively develop and utilize this natural resource, the present invention first studies the red pigment extraction method, the stability of physical and chemical properties, and biological activity of osmanthus osmanthus seeds, and has successfully extracted the red pigment from osmanthus osmanthus seed skins.

The preparation method of the red pigment that extracts from sweet-scented osmanthus seed skin of the present invention, its steps are as follows:

1) Put the sweet-scented osmanthus seed skin into an acidic polar solvent to extract; the polar solvent can be selected from water, or ethanol, or acetone, etc., and can also be mixed in any proportion by several polar solvents If ethanol is used, it is best to use an ethanol solution with a volume concentration of 20-90%; in order to achieve better extraction quality and consider the stability of the pigment, the extraction temperature can be controlled at 20-100°C; The cost of industrial production, the extraction time can be controlled within 10-40 minutes; because the red pigment of the present invention can exist stably under acidic conditions, but too weak acidity will affect the extraction efficiency, so the pH value of the polar solvent can be 1-5 , the best pH value is 1-4;

2) filtering the obtained extract;

3) After the filtrate is evaporated, the residue obtained is the red pigment extracted from the osmanthus osmanthus seed skin.

The red pigment of the present invention extracted by the above method can show the following physical and chemical properties:

(1) Appearance: the paste is reddish-brown, and the solution is orange-red after being dissolved in a solvent;

(2) UV-visible absorption spectrum: the osmanthus osmanthus seed skin red pigment ultraviolet of the present invention is carried out ultraviolet spectrum scanning, and its ultraviolet-visible absorption spectrum is shown in Fig. 1, as can be seen from Fig. 1, it is 283.5nm place to have a typical in ultraviolet region wavelength There is also a typical absorption peak at the wavelength of 536nm in the visible light region;

(3) Solubility: soluble in water, ethanol, acetone and other polar solvents, slightly soluble in ether and ethyl acetate, almost insoluble in petroleum ether;

(4) In an acidic solution, the smaller the pH value of the solution, the deeper the red; the pH value is below 4, the solution is red, 4<pH<7, the color of the pigment solution gradually changes from light red to colorless; the pH value is greater than After 7, as the pH value increases, the color gradually changes from colorless, light yellow, dark yellow to yellow-green;

(5) Color value: E ^1% _1cm 283.5=44.6.

The following experiments on the biological activity and physical and chemical properties of the red pigment of the present invention take the maximum absorption peak in the ultraviolet region at 283.5nm as the wavelength for measuring absorbance.

(1) to the biological activity assay of red pigment of the present invention:

First prepare red pigment solution of the present invention:

Take 0.10 g of the red pigment of the present invention, dissolve it in 95% ethanol by volume concentration, and prepare a natural red pigment solution with a concentration of 0.5 mg/ml, 0.8 mg/ml, and 1.2 mg/ml.

Measuring of red pigment free radical scavenging ability of the present invention:

a. Determination method:

Add 0.2ml of ethanol solution with mass concentration of red pigment of 0.5mg/ml, 0.8mg/ml and 1.2mg/ml to 8ml of ethanol solution with mass concentration of 0.004% DPPH free radical. Measure its absorbance under 28 ℃, 515nm, measure once every 10min, until reaction reaches equilibrium (absorbance change is little), measure the absorbance of the ethanol solution that does not contain the mass concentration 0.004% DPPH free radical of red pigment of the present invention simultaneously, press as follows The formula calculates the SC % clearance value. The preparation method of BHT solution is the same as the above-mentioned method.

b. Measurement results:

It can be seen from the table below that the red pigment of the present invention has good free radical scavenging ability, and the scavenging rate increases with the increase of mass concentration and adding time, and the scavenging effect is most obvious at 0-10 minutes, and it approaches the maximum value after 10 minutes. After 30min, mass concentration is respectively 0.5mg/ml, 0.8mg/ml, and the red pigment solution of 1.2mg/ml is respectively 72.12%, 73.62%, 80.00% to free radical scavenging rate; tert-butyl-4-carboxytoluene) was 47.6%.

Table red pigment free radical scavenging ability measurement result of the present invention

(2) Physicochemical properties of the red pigment of the present invention:

First prepare red pigment solution of the present invention:

Experimental measurements show that the red pigment aqueous solution of the present invention with a concentration of 0.14 μg/ml can control the absorbance of the pigment solution at 283.5 nm within 1.00. Therefore, a red pigment solution with a concentration of 0.14 μg/ml was prepared for the determination of the following physical and chemical properties.

1. the influence of temperature on red pigment of the present invention:

test methods:

Place the red pigment solution of the present invention with a concentration of 0.14 μg/ml at 25° C., 50° C., 80° C., and 100° C. at intervals of 0.5 h, take it out and quickly cool it to room temperature (generally to 20-25° C.) , Measure its ABS value at the maximum absorption wavelength of 283.5nm and observe the change of ABS value before and after heating.

The measurement results:

As can be seen from Table 1, the absorbance value of the red pigment of the present invention does not change substantially below 100°C. The thermal degradation is 0 within the error range, and the color of the visual pigment solution does not change, which shows that the red pigment of the osmanthus fragrans seed skin of the present invention is under different temperatures. Good thermal stability.

The influence effect of table 1 temperature on red pigment of the present invention

② pH value stability of the red pigment of the present invention:

test methods:

Use HCl and NaOH solutions to adjust the pH of the 0.14 μg/ml red pigment solution of the present invention to be 2, 3, 4, 6, 8, 10, 12 and 13 respectively. After standing for 20 minutes, use UV- A 1100 ultraviolet-visible photometer performs spectral scanning, detects the peak and records the color change of the pigment, and investigates the influence of the pH value on the red pigment of the present invention.

The measurement results:

Plant pigments are generally affected by the pH value. With the change of pH value, the -OH, -C=O-, -O- and other groups in the pigment molecule will undergo nucleophilicity under the action of H ⁺ or OH ^- Or electrophilic rearrangement reaction, the pigment molecules will undergo corresponding structural changes, resulting in color changes. When the amount of change reaches a certain concentration, it will show a stable color outside, which is the root cause of the different colors of plants.

As can be seen from Table 2, the acid-base environment at different pH values has a greater impact on the maximum absorption wavelength and stability of the red pigment of the present invention. When pH=2～7.5, the maximum absorption peak will be blue-shifted. When pH=7.5～12, the maximum absorption peak will appear red-shifted. Under acidic conditions, the absorbance value is small, and the pigment is red, while under alkaline conditions, the absorbance value is large, and the pigment is light green, indicating that the pigment group has changed under different pH environments, making the ABS value change.

The influence of table 2pH value on red pigment stability of the present invention

pH value Maximum absorption peak λmax ABS value 2 283.5 0.390 3 223.0 0.118 4 223.0 0.107 6 224.0 0.801 8 283.O O.405 10 203.0 1.939 12 201.5 1.948 13 206.5 1.968

3. oxidizing agent, reducing agent are to the stability of red pigment of the present invention

test methods:

Take 10ml of the red pigment aqueous solution of the present invention at 0.14 μg/ml and add them to different concentrations of KMnO ₄ , K ₂ CrO ₇ , 10% H ₂ O ₂ , and NaClO solutions, shake well, and measure the ABS value at the maximum absorption wavelength;

Take 0.625g of KMnO ₄ , K ₂ Cr ₂ O ₇ crystals, dissolve them in water, and prepare a 1.25 mg/L KMnO ₄ , K ₂ Cr ₂ O ₇ solution. H ₂ O ₂ solution is diluted 100 times with 30% H ₂ O ₂ solution to obtain 3% H ₂ O ₂ solution; NaClO solution is diluted 50 times with chlorine content>10%. (The concentration of the solution is just to make the absorbance get a better value at 283.5nm)

The measurement results:

The results in Table 4 show that: with the increase of the concentration of KMnO ₄ , K ₂ Cr ₂ O ₇ , 10% H ₂ O ₂ , and NaClO, the absorbance value decreases and the pigment is damaged to varying degrees. It turns colorless after long-term storage, indicating that KMnO ₄ , K ₂ Cr ₂ O ₇ , 10% H ₂ O ₂ , and NaClO all have a bleaching effect on the red pigment of osmanthus osmanthus seeds, or that there are easily oxidized components in the red pigment structural unit. Therefore, when using the red pigment of the present invention, contact with strong oxidizing agents should be avoided as much as possible.

The influence of table 4 oxidizing agent on the stability of red pigment of the present invention

test methods:

In 5 test tubes, add 0.01, 0.02, 0.05, 0.10, 0.15 grams of Na ₂ SO ₃ , then add 10 ml of 0.14 μg/ml red pigment aqueous solution of the present invention, shake well, and measure the ABS value at the maximum absorption wavelength;

The measurement results:

It can be seen from Table 5 that different amounts of Na ₂ SO ₃ have no obvious influence on the absorbance of the red pigment of osmanthus fragrans seeds of the present invention, and it can be seen that the red pigment of the present invention is relatively stable in the reducing agent.

When table 5 adds different amounts of reducing agent (Na ₂ SO ₃ ), the red pigment absorbance change value of the present invention

4. metal ions are stable to the red pigment of the present invention

test methods:

Prepare CuSO ₄ , MgSO ₄ , CaCl ₂ , NaSO ₄ , FeCl ₃ , AlCl ₃ and ZnCl ₂ solutions to make their concentrations reach 30mg/L, and add 0.2ml of these 7 kinds of metal ions to 10ml respectively to 0.14μg/L ml of the red pigment solution of the present invention, measure the ABS value of the metal ion pigment solution at the maximum absorption wavelength.

The measurement results:

Table 6 shows that the red pigment of the present invention reacts differently to seven kinds of metal ions. The pigment has a strong fading effect on Cu ²⁺ , Ca ²⁺ , Fe ³⁺ , Al ³⁺ , Zn ²⁺ ; but Mg ²⁺ , Na ⁺ has a certain color retention and color enhancement effect on the red pigment of the present invention.

Table 6 metal ions are to the pigment stability of the present invention

time distilled water Cu ²⁺ Mg ²⁺ Ca ²⁺ Na ⁺ Fe ³⁺ Al ³⁺ Zn ²⁺ 10min absorbance 0.759 0.759 0.745 0.748 0.768 0.769 0.770 0.762 Absorbance at 39h 0.329 0.474 0.664 0.500 0.676 0.422 0.251 0.452 48h absorbance 0.310 0.196 0.476 0.192 0.465 0.256 0.233 0.185 Degradation rate 59.1% 74.2% 36.1% 74.3% 39.5% 66.7% 69.7% 75.7%

5. Factors such as sugar, amino acid, organic acid, starch to red pigment stability of the present invention

test methods:

Sugar (glucose and sucrose), amino acid (L-glutamic acid), organic acid (citric acid and tartaric acid), and starch are configured into a solution with a mass concentration of 1%. Invent red pigment solution 10ml, measure the ABS value every 20min at 95°C.

The measurement results:

As seen in Table 7, amino acids (L-glutamic acid), organic acids (citric acid and tartaric acid), starch, sucrose and glucose have no effect on the stability of the red pigment of osmanthus fragrans seeds, and all have a certain color-enhancing effect, so this pigment is suitable in most foods.

Table 7 amino acids, organic acids, factors such as starch influence on the red pigment stability of the present invention

⑥ Salt tolerance of the red pigment of the present invention

test methods:

In 3 test tubes, add 0.05g, 0.10g, 0.25g table salt respectively, add 10ml of the red pigment solution of the present invention with a concentration of 0.14μg/ml, shake well. Measure the absorbance.

The measurement results:

The absorbance values of the red pigment of the present invention in different concentrations of sodium chloride test solution are shown in Table 8, and the results show that the red pigment of the present invention has good stability to the food matrix of sodium chloride.

Table 8 red pigment salt tolerance test result of the present invention

7. The demarcation of red pigment color value of the present invention

test methods:

The color value of the red pigment of the present invention adopts the regulations of the National Food Additives Standardization Technical Committee, and all natural colorants are used. 10ml was diluted 100 times, and the absorbance was measured at a wavelength of 283.5 with a 1cm cuvette and a UV-1100 ultraviolet-visible photometer. It is required to measure the absorbance of the solution concentration and express the absorbance of a certain concentration between 0.2 and 0.7. Generally, 1wt%, 1cm cuvette is used, and the absorbance measured at the maximum absorption peak (283.5nm) is expressed in the form of color value = E ^1% _1cm 283.5 absorbance.

The measurement results:

Experimentally measured absorbance A=0.446

Color value=E ^1% _1cm 283.5=A×100/w=44.6, which is greater than the current national standard such as the color value standard of radish red pigment (E ^1% _1cm λmax=44.0), while the color value of general pigments is also around 45.0 . Illustrate that the content and the purity of the osmanthus seed cortex red pigment extracted by the preparation method of the present invention are higher.

The red pigment extracted from the osmanthus osmanthus seed skin of the present invention has good water and alcohol solubility and strong tinting power. It can be seen from the above-mentioned various measurements that it has good thermal stability, oxidation resistance and reduction, sugar and salt tolerance. , Amino acid, organic acid, starch and various metal ions such as Mg ²⁺ , Na ⁺ etc. can add color to it, and the color value can be greater than 40. In addition, it has a strong ability to scavenge free radicals. And the red pigment of the present invention is the extract of natural plants, safe, without any toxic and side effects, and has a wide range of uses. It can be used as a food coloring in the food processing industry, as a coloring agent for food and beverages, as well as medicines and health care products. It can be used as a coloring agent for sugar-coated tablets; or it can be used as an additive for cosmetics to prepare various cosmetics such as lipstick, blush, etc. It can also be used as a coloring agent for hair dyeing and nail dyeing, and it can also be used as an industrial coloring agent Agents, dyes and biological dyes, biological reagents.

(4) Description of drawings:

Fig. 1 is the red pigment ultraviolet-visible absorption spectrogram that the present invention extracts from sweet-scented osmanthus seed skin.

(5) Specific implementation methods:

Example 1:

Put the pulverized osmanthus seed skin into an ethanol solution with a pH=4 and a volume concentration of 90%, and extract at 100°C for 10 minutes;

2) the obtained extract is cooled and suction filtered;

3) After the filtrate is evaporated, the residue obtained is the red pigment extracted from the osmanthus osmanthus seed skin.

Example 2:

1) Put the pulverized osmanthus seed skin into an acetone solution with a pH=1 and a volume concentration of 20%, and extract at 80° C. for 40 minutes;

2) the obtained extract is cooled and suction filtered;

3) After the filtrate is evaporated, the residue obtained is the red pigment extracted from the osmanthus osmanthus seed skin.

Example 3:

1) Clean the fresh osmanthus seed skins and peel off the skins. After washing the pulp, put the skins into an oven to dry at 80°C. The obtained dried osmanthus seed skins are crushed and put into water, and hydrochloric acid is added to make the pH = 2 , extracted at 20°C for 40 minutes;

2) cooling and filtering the obtained extract;

3) After the filtrate is evaporated, the residue obtained is the red pigment extracted from the osmanthus osmanthus seed skin.

Example 4:

1) After cleaning the fresh osmanthus seed skin, remove the epidermis, put the skin after washing off the pulp into an oven and dry it at 60° C., put the dried osmanthus seed skin into a powder with pH=5 and a volume concentration of 60% after being crushed. In acetone solution, extract at 40°C for 10 minutes;

2) cooling and filtering the obtained extract;

3) After the filtrate is evaporated, the residue obtained is the red pigment extracted from the osmanthus osmanthus seed skin.

Example 5:

1) Put the dried and pulverized osmanthus seed skin into an ethanol solution with a pH of 2 and a volume concentration of 50%, and extract at 60° C. for 30 minutes;

2) filtering the obtained extract;

3) After the filtrate is evaporated, the residue obtained is the red pigment extracted from the osmanthus osmanthus seed skin.

Embodiment 6:

1) Put the osmanthus seed skin into a methanol solution with a pH=3 and a volume concentration of 80%, and extract at 50°C for 20 minutes;

2) filtering the obtained extract;

3) After the filtrate is evaporated, the residue obtained is the red pigment extracted from the osmanthus osmanthus seed skin.

Embodiment 7:

1) Clean the fresh osmanthus seed skin and remove the epidermis, put the skin after washing off the pulp into an oven for drying at 80°C, crush 10 g of the dried osmanthus seed skin and put it into pH=2, volume concentration 90% In the ethanol solution, extract at 80°C for 40min;

2) cooling and suction filtering the obtained extract;

3) After the filtrate was evaporated, 3.46 g of residue was obtained, which was the red pigment extracted from the osmanthus osmanthus seed skin.

Experimental Example 1: Adding red pigment to drinks

Method: Dissolve 0.01 g of the red pigment described in Example 7 into 100 ml each of commercially available liquor and cola drinks, observe the changes in appearance, and measure the changes in absorbance regularly.

Result: the red pigment is added to cola and white wine and placed indoors, and its pigment retention rate still reaches 79.5% and 82% in 30 days, and the appearance is almost unchanged, which shows that the red pigment described in Example 7 is relatively good in these two drinks It is stable and can be widely used in the coloring of liquid beverages, etc.

The absorbance measurement results are shown in the table below:

drinks 0 days 1 day 2 days 3 days 4 days 5 days 6 days 7 days 14 days 30 days Cola 0.694 0.678 0.670 0.654 0.650 0.634 0.625 0.615 0.594 0.552 Liquor 0.815 0.803 0.794 0.780 0.765 0.760 0.743 0.737 0.694 0.668

Experimental example 2: red pigment of the present invention is used for the dyeing of candied fruit

Recipe: 100g of red bayberry base, 54g of white sugar, 3.1g of licorice powder, 0.05g of citric acid, 0.05g of potassium sorbate, 0.05g of the red pigment described in Example 7.

Process: Soak the plum base in clean water for 1 hour (change the water three times during the period), remove and drain the water after cleaning. Take 4/5 of the total weight of white sugar, add equal weight of water to boil and dissolve to make sugar liquid, steam heat, pour bayberry billet into sugar liquid, stir evenly, start the pot, sprinkle the remaining white sugar on the surface, soak for 24 hours, remove Drain the sugar solution. Boil the drained sugar liquid to thicken, add licorice powder, preservatives, red pigment and citric acid, stir well, pour the dipped red bayberry base, mix well and then soak for 48 hours. After marinating, drain and pack, it is the finished product.

Result: The dried red bayberry with uneven color can be deepened and uniform in color, strong in coloring power, and has no toxic side effects.

Experimental example 3: the red pigment of the present invention is used for the preparation of hair dye

Formula: 2.0 grams of the red pigment described in Example 7, 21.0 grams of isopropanol, 6.0 grams of benzyl alcohol, 1.0 grams of citric acid, 20.0 grams of 5% hydroxymethylcellulose aqueous solution, and 50.0 grams of deionized water.

Process: Dissolve isopropanol, benzyl alcohol and citric acid in deionized water first, add red pigment, and finally add 5% hydroxymethyl cellulose aqueous solution, stir well and serve.

Result: After use, there are no toxic side effects, no irritation, no allergies, no damage to hair quality, natural and bright color, firm coloring, not easy to fade and change color, and the hair is soft and shiny. Long-term use can also protect and strengthen hair And the effect of eliminating dandruff.

Experimental Example 4: Adding the red pigment of the present invention into cosmetics

Lipstick base material is weighed, and pigment is got bromate red 5.0%, the red pigment 0.2% described in embodiment 7 to mix color by the weight percentage of lipstick gross weight, through heating, mixing, casting, cooling, solidify form lipstick, For the bright red lipstick.

Result: It can nourish the lips, effectively retain moisture, and has no greasy feeling, is not easy to fade, and has no toxic side effects.

Claims (9)

1. the preparation method of the red pigment that extracts from sweet-scented osmanthus seed skin, its step is as follows: 1) Putting sweet-scented osmanthus seed skin into an acidic polar solvent to extract; wherein, the polar solvent is water, ethanol, acetone, hydrochloric acid or methanol; 2) filtering the obtained extract; 3) After the filtrate is evaporated, the residue obtained is the red pigment extracted from the osmanthus osmanthus seed skin. 2. the preparation method of the red pigment that extracts from osmanthus fragrans seed skin according to claim 1, is characterized in that: in step 1), the pH=1～5 of polar solvent. 3. the preparation method of the red pigment that extracts from osmanthus fragrans seed skin according to claim 2 is characterized in that: in step 1), the pH=1～4 of polar solvent. 4. The preparation method of the red pigment extracted from osmanthus osmanthus seed skin according to any one of claims 1-3, characterized in that: in step 1), the extraction temperature is 20-100°C. 5. The preparation method of the red pigment extracted from osmanthus osmanthus seed skin according to any one of claims 1 to 3, characterized in that: in step 1), the extraction time is 10 to 40 minutes. 6. the red pigment that extracts from the osmanthus fragrans seed skin that any one described method prepares in claim 1～5. 7. the red pigment that extracts from sweet-scented osmanthus seed skin according to claim 1 is as the purposes of food coloring. 8. the red pigment that extracts from sweet-scented osmanthus seed skin according to claim 1 is as the purposes of cosmetic additive. 9. the red pigment that extracts from sweet-scented osmanthus seed skin according to claim 1 is as the purposes of dyeing agent.

Images