CN1009797B - Plant bactericide and its preparing method - Google Patents
Plant bactericide and its preparing methodInfo
- Publication number
- CN1009797B CN1009797B CN 88108134 CN88108134A CN1009797B CN 1009797 B CN1009797 B CN 1009797B CN 88108134 CN88108134 CN 88108134 CN 88108134 A CN88108134 A CN 88108134A CN 1009797 B CN1009797 B CN 1009797B
- Authority
- CN
- China
- Prior art keywords
- bactericide
- plant
- herb
- growth regulator
- plant growth
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The present invention provides a novel plant bactericide using the aqueous extract of natural traditional Chinese medicine herb as the basic ingredients, and a preparing method thereof. In addition, the present invention also provides an application method of the plant bactericide or the mixture of the plant bactericide in preventing and curing the diseases of plants, especially vegetables, and increasing the yield of the plants.
Description
The present invention relates to a kind of bactericide, particularly relating to a kind of is the new type bactericide of ultimate constituent and its production method with the Chinese herbal medicine extract, and this bactericide is in the particularly application in the vegetable disease of control plant.
Now, along with developing rapidly of agricultural production, the use of agrochemicals such as various chemical pesticide and chemical fertilizer is more and more general, and usage amount is also increasing, and in the world ecotope and soil quality caused very big threat.Particularly some chemical substance also has certain harm to human body, thereby jeopardizes agricultural producer and consumer's health.
In vegetables produce, in order to prevent and treat the various diseases of vegetables, increase yield of vegetables, developed many bactericides so far, commonly used if any carbendazim, metalaxyl, aliette, zineb, ambam, tpn etc., but the active ingredient of these agricultural chemicals all is the chemical substance of synthetic, can cause the chemical residual of the edible part of vegetables after using, and in a single day these residual chemical substances are taken in by human body, just can cause murder by poisoning to human body, jeopardize consumer's health.Therefore, development " nuisanceless " vegetables have become an important topic in the current vegetables production.Correspondingly, in vegetable disease control, just press for and find a kind of both controlling plant diseases effectively, can reduce or eliminate the novel agrochemical of chemical contamination again.
Based on above-mentioned consideration, the inventor can be used for preventing and treating the human or animal because of fungal infection, this fact of the caused disease of bacterium according to the active ingredient of some medicinal plant (Chinese herbal medicine), infer that the certain plants disease also may have susceptibility to the active ingredient of these medicinal plants, and these natural materials may be used to prevent and treat fungi or the bacterial disease of plant such as vegetables.
We find uncannily in the research, and the extract of some medicinal plant has remarkable prevention effect really to some fungi or the bacterial disease of vegetables such as cucumber, tomato, green pepper, Leek dish etc., thereby has caused the present invention.
It is the novel plant bactericide of active ingredient, its composition and preparation method thereof that the present invention at first provides a kind of extract with natural medicinal plant-Chinese herbal medicine.
Having the present invention further provides a kind of is solvent with said bactericide, and is aided with certain plant growth regulators and novel plant sterilization-growth regulating mixture of making and preparation method thereof.
The present invention also provides and has used above-mentioned bactericide or plant sterilization-growth regulating mixture, with the method for control vegetable disease, promotion vegetable growth.
Extract in the natural medicinal plant of bactericide of the present invention system or artificial planting wild by following six kinds of Chinese Jilin governor Xiang Shan areas, they are: the burdock leaf that the florescence gathers, the thyme herb that the florescence gathers, the greater celandine herb that the florescence gathers, the rhizoma calami root that gather autumn, the garden burnet root that gather autumn and the tobacco stem of gathering winter.The above-mentioned medicinal plant that is used to prepare bactericide is the product that dry in the shade, and their dry weight ratio is respectively burdock leaf 10～15%, thyme herb 15～25%, the white grass 1～10% of greater celandine, rhizoma calami root 10～20%, garden burnet root 5～25%, tobacco stem 30～40%.
Can prepare bactericide of the present invention as follows: the part of at first will being used as medicine at the medicinal plant of Various Seasonal and collection in vegetative period is dried in the shade and is pulverized.Take by weighing the burdock leaf, rhizoma calami root, greater celandine herb and the tobacco stem that pulverize then according to the above ratio, and adding accounted for the about 100 times water logging bubble of medicinal material gross weight 4～8 hours.Soak time later with very hot oven boil 20～40 minutes, add the thyme herb and the garden burnet root of the pulverizing that takes by weighing in proportion successively again, slow fire boiled 1-2 hour.The cooling back removes by filter residue with the conventional filtration method.Heating concentrates clear liquid then, reaches 10～15% until extract concentrations.Filtered while hot gained concentrate promptly obtains bactericide stoste of the present invention once more.
The bactericide that so makes can use separately, also can mix use with other bactericide and/or plant growth regulator as required.We consider nearly all vegetables of disease having taken place or potential disease being arranged, all exist and suffer pathogenic bacterial infection or other weathers because of planting, soil, the influence of factors such as source of nutrition and the depauperation and the growthing lag phenomenon that cause, particularly we find that the present invention not only has the good preventing vegetative bacteria based on the bactericide of Chinese herbal medicine, the effect of fungal disease, and experiment shows that bactericide of the present invention application effect after using than the disease generation before the disease generation is better, and this moment, plant metabolism was the most vigorous just, grow the fastest period, give bactericide and plant growth regulator in this period simultaneously, will both receive simultaneously and can prevent and treat disease, can regulate plant metabolism again, increase photosynthesis of plant, make the double effects of vegetables increasing both production and income.For this reason, we are providing on the basis of above-mentioned bactericide, and further providing more than one to state bactericide is basis, is aided with the plant growth regulator of special preparation, the new plant sterilization-growth regulating mixture that makes with simple mixed method.
Can use simple mixed method to prepare plant sterilization of the present invention-growth regulating mixture.For example can add each component successively in aqueous medium: bactericide 10～15%(V/V), plant growth regulator (for example triacontanol) 5～10%(V/V), Sodium Benzoate 0.2～0.4%(V/V) add water and supply volume behind the mixing.Also can be in the process of aforementioned preparation bactericide, after the concentrate filtered while hot, be heated to once more and boil, treat that temperature adds acceptable excipient on an amount of preservative sodium benzoate and other plant physiologies when reducing to 80～90 ℃, add plant growth regulator (for example triacontanol) when continuing to be cooled to 30～35 ℃, thereby obtain plant sterilization of the present invention-growth regulating mixture.
Said " plant growth regulator " can be any medicament with plant growth stimulating effect, but the plant growth regulator (Chemical Plant of Xiamen Univ.) of the best commodity in use of the present invention " triacontanol " by name, it has the effect of significant stimulating plant metabolism, enhancing photosynthesis, promotion plant growing for a kind of natural plants growth stimulant that extracts from honeybee is cured.It is the same with bactericide of the present invention, also is to extract from nontoxic natural material, therefore also is a kind of desirable agricultural chemicals material that is suitable for producing " nuisanceless " vegetables.In addition, experiment also show this natural plants growth stimulant and plant sterilization of the present invention use with only can produce that work uses assist the effect that multiplies each other mutually, and without any antagonism or other adverse effects.
In actual the use, can by conventional techniques bactericide of the present invention or plant sterilization-growth regulating mixture be become multiple different formulations such as the aqueous solution, emulsifier, spray, microcapsules as required, but, preferably make aqueous pharmaceutical for the convenience on using.In general, in the aqueous solution content of water between 99～80%.
Can use any ways customary that plant is used bactericide of the present invention or plant sterilization-growth regulating mixture, but preferably adopt spraying method,, use manually or power driven sprayer is sprayed on the blade face of plant promptly at plant premorbid and/or their early stage.Can use the water of 1: 200～400 times of dilutions to become dilution when being used for the treatment of after the plant morbidity, every about 7 days, spray once, 2-4 time altogether; When being used to prevent, can use the water of 1: 400～600 times of dilutions to become dilution, every about 5 days, spray once, use 2-3 time altogether.
Plant sterilization of the present invention-growth regulating mixture system is a basis with natural medicinal plant (Chinese herbal medicine) extract, being aided with is the plant growth substance that extracts from natural material equally, can be used for preventing and treating plant, particularly fungi of vegetables or bacterial disease, both vegetable disease can be prevented and treated effectively, the output of vegetables can be improved to a certain extent again.In addition, what should particularly point out is, bactericide of the present invention or plant sterilization-growth regulator are different from all kinds of chemical synthetic pesticides that generally use at present, it can not work the mischief to the natural ecological balance and soil quality because of using in a large number, can be in the crops chemical substance of residual harmful on the vegetables particularly, jeopardizing agricultural producer and consumer's health, therefore is the desirable agricultural chemicals of producing " nuisanceless " vegetables.
We experimental results show that by spraying of farmland and chamber vegetable crop, bactericide of the present invention or plant sterilization-growth regulator can be used for preventing and treating bacterium, the fungal disease of various vegetables, as cucumber downy mildew, bacillary blue pinta, tomato early blight or late blight, green pepper blister scall, Leek dish gray mold, and folium panacis japonici cum caule, stem spot disease and other plant are by fungi, bacterial disease.Experimental result shows, is used to prevent and treat cucumber downy mildew, bacillary blue pinta is efficient reaches 70.12～88.3%; Be used to prevent and treat tomato early blight, late blight is efficient reaches 71.2～81.3%; Be used to prevent and treat the folium panacis japonici cum caule, the stem spot disease is efficient reaches 70.3～80.1%.
Use bactericide of the present invention, can make full use of the natural Chinese medicinal herb resource, reduce production costs; It is respond well to be used to prevent and treat vegetable disease, and under the situation of using mixture, also has the effect that increases yield of vegetables concurrently.Nontoxic (the LD of bactericide particularly of the present invention
50=18,272,585mg/kg body weight) harmless, pollution of ecological environment does not have chemical residual on the edible part of vegetables, and human body can not cause adverse effect after taking in, and can be widely used in the production of pollution-free vegetable and other crops.
In addition, show on evidence that also bactericide of the present invention has the double preferably property controlled to vegetable disease, so germ is difficult for it is developed immunity to drugs; The opposite chemosynthesis bactericide that generally uses at present then all has stronger treatment selectivity, uses the back germ to be easy to develop immunity to drugs repeatedly.
Embodiment 1
The preparation of bactericide
Take by weighing the burdock leaf 15g of drying, pulverizing respectively;, greater celandine herb 5g, rhizoma calami root 15g, tobacco stem 35g mixes the back and adds 10 premium on currency and soaked 6 hours; the danger fire boils the back and adds thyme herb 20g and garden burnet root 10g dry, that pulverize, and slow fire decocts 1.5 hours.With four layers of hospital gauze filtering dregs of a decoction, extract heating is concentrated, detecting wherein DDGS concentration with absorption spectrometry is promptly to stop heating at 10～15% o'clock, it is standby that cooling is placed on low temperature under preservation.
Embodiment 2
The preparation of plant sterilization-growth regulating mixture
Get bactericide 12ml by the preparation of embodiment 1 method, add plant growth regulator " triacontanol " (Chemical Plant of Xiamen Univ.) 8ml, Sodium Benzoate 200 μ l, add water 79.8ml then, mixing promptly obtains plant sterilization-growth regulator of the present invention under the room temperature.
Embodiment 3
Bacteriostatic test (1)
Get the blue pinta pathogen of quantitative cucumber suspension, evenly inoculation will soak the low dish suction of soup and go to be positioned on the flat board behind the unnecessary soup on the PDA culture medium flat plate, and each is organized equal three times and repeats; Randomized arrangement is in 28 ℃ of incubators, respectively at 24,48 hours detection antibacterial circle diameter sizes, with the inhibitory action of clear and definite plant sterilization mixture of the present invention to the blue pinta pathogen of cucumber.The results are shown in down in the tabulation 1.
Table 1
The medicament average diameter of inhibition zone
24 hours 48 hours
Plant sterilization mixture (producing in 88 years) 6.09 6.15
Plant sterilization mixture (producing in 87 years) 5.76 6.08
But 30%DT wet-milling 7.07 6.25
The CK(clear water) 00
By the result shown in the table 1 as can be seen: (1) plant sterilization mixture of the present invention is compared with the DT bactericide, and fungistatic effect is low slightly; (2) product of the present invention stores that its fungistatic effect has decline slightly more than 1 year; (3) bactericide bacteriostasis of the present invention has increase slightly after the prolongation processing time, descends but DT then shows bacteriostasis.
Embodiment 4
Bacteriostatic experiment (2)
Picking sporangium from the cucumber leaves that has the downy mildew spore places the soup and the clear water that prepare, and each group all contains the full ascus number (40～50 in every visual field) of basically identical.The quantitative bacterium drop that will have spore then is added on the single hole concave slide, places 25 ℃ of incubators to make it to sprout.4, the microscopy sporangium is sprouted number after 24 hours.Check 5 visuals field for every group, the full subnumber order that moves about that relatively discharges the results are shown in the table 2.
Table 2
Handle the sporangium number and discharge the full subnumber inhibition effect (%) of moving about
Ⅰ Ⅱ Ⅲ Ⅹ
Plant sterilization mixture 225 7 10 12 9.6 96
Metalaxyl 239 0000 100
The CK(clear water) 231 131 127 199 152.3 0
By the result shown in the table 2 as can be seen: (1) bactericide of the present invention can suppress the sporangial sprouting of cucumber downy mildew significantly; (2) the inhibition effect of bactericide of the present invention is worse than metalaxyl slightly, but results of statistical analysis shows both there was no significant differences.
Embodiment 5
The control efficiency experiment
Control experiment to blue pinta of cucumber and cucumber downy mildew is carried out in the little test block of greenhouse and the earth respectively.When cucumber leaves first meeting scab, begin to spray medicine for the first time, resprayed once every 6 days later on, spray altogether 4～5 times.Each is organized equal three times and repeats.Not inferior spraying selected the middle part blade to check control efficiency in back 6 days, the results are shown in the table 3.
By last tabulation 3 as can be seen: (1) plant sterilization mixture of the present invention to the control efficiency of the bacillary blue pinta of cucumber apparently higher than metalaxyl, a little more than the DT bactericide; To the control efficiency of cucumber downy mildew apparently higher than the DT bactericide, and a little less than metalaxyl; (2) plant sterilization mixture of the present invention not only has the effect of remarkable sterilization, control vegetable disease, and than using the effect (output increases about 7.8%) that other known simple agricultural chemicals also have concurrently increases yield of vegetables.
Claims (7)
1, a kind of novel plant bactericide, the active ingredient that it is characterized in that bactericide are natural Chinese medicinal herb material burdock leaf, thyme herb, the water extract of greater celandine herb, Ge Pugen, garden burnet root and tobacco stem,
Weight (dry weight) ratio that wherein is used to prepare the Chinese medicinal herbs of this bactericide is respectively burdock leaf 10-15%, thyme herb 15-25%, greater celandine herb 1-10%, calamus root 10-20%, garden burnet root 5-25%, tobacco stem 30-40%.
2, according to the bactericide of claim 1, wherein also can contain any plant growth regulator.
3, according to the bactericide of claim 2, wherein said plant growth regulator extracts from natural bee is cured.
4, a kind of preparation is according to each the bactericide or the method for mixture among the claim 1-3, and it comprises:
(1) burdock leaf, Chang Qian root, greater celandine herb, the tobacco stem pulverized are soaked and boiled;
(2) add thyme and garden burnet root, continue to decoct;
(3) add acceptable additive on the concentrated an amount of plant growth regulator of gained and other agricultural chemicals after filtration and the concentrated gained clear liquid.
5,, wherein can add or not add plant growth regulator according to claim 4 method.
6, " plant growth regulator " in claim 2,3 and 5 is the auxin that extracts from natural material.
7, according among the claim 1-4 each bactericide or mixture in the particularly application on the vegetable disease of control plant.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 88108134 CN1009797B (en) | 1988-12-06 | 1988-12-06 | Plant bactericide and its preparing method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 88108134 CN1009797B (en) | 1988-12-06 | 1988-12-06 | Plant bactericide and its preparing method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1035225A CN1035225A (en) | 1989-09-06 |
| CN1009797B true CN1009797B (en) | 1990-10-03 |
Family
ID=4834955
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 88108134 Expired CN1009797B (en) | 1988-12-06 | 1988-12-06 | Plant bactericide and its preparing method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1009797B (en) |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105076253B (en) * | 2015-08-26 | 2018-01-09 | 山东省农业科学院农业质量标准与检测技术研究所 | A kind of cucumber specific complex type growth regulator |
| CN105724130A (en) * | 2016-01-28 | 2016-07-06 | 高贤泽 | Method for controlling cucumber bacterial angular leaf spots based on biology experiments for senior high school |
| CN107488059A (en) * | 2017-07-28 | 2017-12-19 | 惠州市锦恒工业模具设计合伙企业(普通合伙) | A kind of Bactericide fertilizer based on turmeric root tuber extract |
| CN107494635A (en) * | 2017-08-15 | 2017-12-22 | 赖进九 | For preventing and treating cucumber disease and improving the plant extracts of cucumber nutrient composition content |
| CN107500876A (en) * | 2017-09-02 | 2017-12-22 | 张颂忠 | A kind of medicine fertilizer and preparation method thereof |
| CN108244157B (en) * | 2018-01-24 | 2020-03-31 | 黑龙江省动物疫病预防与控制中心 | Biological disinfectant for animals |
| CN108283120A (en) * | 2018-05-02 | 2018-07-17 | 佛山市日可威食品科技研究院（普通合伙） | A kind of Paris polyphylla inoculation method rich in various trace elements |
| CN108850022A (en) * | 2018-06-29 | 2018-11-23 | 松桃鑫山农业科技开发有限公司 | A kind of composition and preparation method thereof for preventing and treating tomato early blight |
| CA3145688A1 (en) * | 2019-07-25 | 2021-01-28 | Guy Chamberland | Methods and compositions of a botanical extract to promote and boost plant growth and prevent and suppress plant diseases |
-
1988
- 1988-12-06 CN CN 88108134 patent/CN1009797B/en not_active Expired
Also Published As
| Publication number | Publication date |
|---|---|
| CN1035225A (en) | 1989-09-06 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102649654B (en) | Traditional Chinese medicine organic fertilizer | |
| AU4648793A (en) | Organic pesticide | |
| CN105076253B (en) | A kind of cucumber specific complex type growth regulator | |
| CN106490057A (en) | A kind of composite pesticide for Rice Cropping and preparation method thereof | |
| CN108552012A (en) | A kind of matrix and preparation method thereof being suitable for ginger cultivation | |
| CN104496668A (en) | Green organic chemical fertilizer and preparation method thereof | |
| Ahmad et al. | Effect of bio-fertilization and some plant extracts on the growth, yield and chemical constituents of basil plant | |
| CN1009797B (en) | Plant bactericide and its preparing method | |
| CN106478275A (en) | A kind of composite pesticide of preventing and treating tobacco diseases and preparation method thereof | |
| CN108849875A (en) | A kind of biology base porous carbon plants essential oil controlled release agent, preparation method and applications preventing and treating soil-borne disease of crop | |
| CN106259458A (en) | A kind of pesticide yield-increasing compositions containing jasmonic and (-)-methyl cis-2-pent-2'-enyl-3-oxocyclopentylacetate | |
| CN104892146B (en) | A kind of fertilizer for being applied to the strong fruiting period of orange tree and applying and preparation method thereof | |
| CN106748099A (en) | Celery special insect-proof foliar fertilizer and preparation method thereof | |
| CN106234467A (en) | A kind of antibacterial and preparation method thereof | |
| CN116210728B (en) | Bactericide and preparation method and application thereof | |
| CN109907080A (en) | A kind of organic vegetable pest control chemicals and its preparation and application | |
| CN105315071A (en) | Humic acid type water-retaining agent utilizing vinegar factory waste liquid, and preparation method for humic acid type water-retaining agent | |
| CN109265264A (en) | It is capable of the peanut cultivation special fertilizer of controlling disease | |
| CN109608269A (en) | A kind of dedicated ecological bacterial manure of rice and its production method | |
| CN106490048A (en) | A kind of environment friendly agricultural of preventing and treating brassicaceous vegetable pest and disease damage and preparation method thereof | |
| CN109169736A (en) | It is a kind of using peppermint and balsam pear as the pest repellant of substrate and preparation method, application | |
| CN108684723B (en) | Disease-preventing composition special for rice | |
| CN102365939A (en) | Botanical sterilization preparation and application thereof for controlling bitter rot or anthracnose of grape | |
| Zeljković et al. | The effect of water extract of Aloe vera (L.) Burm. f. on germination and growth of scarlet sage. | |
| CN106495862A (en) | A kind of composite pesticide for apple tree cultivation and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C13 | Decision | ||
| GR02 | Examined patent application | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C19 | Lapse of patent right due to non-payment of the annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |