Embodiment
In order to understand essence of an invention better, describe the technology contents of invention in detail below in conjunction with specific embodiment.
The preparation of embodiment 1:2.5% rotenone+emamectin missible oil, each composition weight proportioning is:
Rotenone is 0.05%;
Emamectin is 2.45%;
Surfactant: the dodecyl phenol polyethenoxy ether is 15%;
Cosolvent: methyl alcohol is 15%;
Anti-photodissociation agent: castor oil is 1%;
Stabilizing agent: epoxidized soybean oil is 17%;
Penetrating agent: Laurocapram is 12%;
Organic solvent: dimethylbenzene is mended to 100%;
Each component is put into modulation kettle mixes well and promptly gets product.
Embodiment 2:3% rotenone+according to the preparation of general rhzomorph missible oil, each composition weight proportioning is:
Rotenone is 0.3%;
According to general rhzomorph is 2.7%;
Surfactant: the dodecyl phenol polyethenoxy ether is 15%;
Cosolvent: methyl alcohol is 15%;
Anti-photodissociation agent: castor oil is 1%;
Stabilizing agent: epoxidized soybean oil is 17%;
Penetrating agent: Laurocapram is 12%;
Organic solvent: dimethylbenzene is mended to 100%;
Each component is put into modulation kettle mixes well and promptly gets product.
The preparation of embodiment 3:3% rotenone+emamectin missible oil, each composition weight proportioning is:
Rotenone is 2.95%;
Emamectin is 0.05%;
Surfactant: the dodecyl phenol polyethenoxy ether is 15%;
Cosolvent: methyl alcohol is 15%;
Anti-photodissociation agent: castor oil is 1%;
Stabilizing agent: epoxidized soybean oil is 17%;
Penetrating agent: Laurocapram is 12%;
Organic solvent: dimethylbenzene is mended to 100%;
Each component is put into modulation kettle mixes well and promptly gets product.
The preparation of embodiment 4:70.5% rotenone+ivermectin wetting powder, each composition weight proportioning is:
Rotenone is 70%;
Ivermectin is 0.5%;
Wetting agent: bay alcohol radical sodium sulphate is 10%;
Dispersant: alkylsulfonate is 10%;
Carrier: kaolin is mended to 100%;
With each component drying, cooling, mixing, the levigate product that promptly gets.
The preparation of embodiment 5:70.5% rotenone+emamectin wetting powder, each composition weight proportioning is:
Rotenone is 0.5%;
Emamectin is 70%;
Wetting agent: bay alcohol radical sodium sulphate is 10%;
Dispersant: alkylsulfonate is 10%;
Carrier: kaolin is mended to 100%;
With each component drying, cooling, mixing, the levigate product that promptly gets.
The preparation of embodiment 6:10% rotenone+emamectin wetting powder, each composition weight proportioning is:
Rotenone is 0.5%;
Emamectin is 9.5%;
Wetting agent: bay alcohol radical sodium sulphate is 25%;
Dispersant: alkylsulfonate is 22%;
Carrier: kaolin is mended to 100%;
With each component drying, cooling, mixing, the levigate product that promptly gets.
The preparation of embodiment 7:30% rotenone+allosamidin oil-suspending agent, each composition weight proportioning is:
Rotenone is 10%;
Allosamidin is 20%;
Dispersant: sodium lignin sulfonate is 10%;
Tackifier: bentonite is 3%;
Stabilizing agent: xanthan gum is 2%;
Defoamer: organic silicone is 5%;
Solvent: castor-oil plant oil subsidy to 100%;
Each component put into send into homogeneous blender mixing after the sand milling still grinds and promptly get product.
The preparation of embodiment 8:20% rotenone+allosamidin microemulsion, each composition weight proportioning is:
Rotenone is 8%;
Allosamidin is 12%;
Emulsifier: alkyl benzene sulfonate calcium salt is 20%;
Stabilizing agent: cresyl glycidyl ether is 13%;
Solvent: butanone is 20%;
Antifreezing agent: sorbierite is 3%;
Deionized water is mended to 100%.
Mixture is stirred in agitator, and the heating phase inversion promptly gets product.
The preparation of embodiment 9:18% rotenone+selamectin microemulsion, each composition weight proportioning is:
Rotenone is 8%;
Selamectin is 10%;
Emulsifier: alkyl benzene sulfonate calcium salt is 20%;
Stabilizing agent: cresyl glycidyl ether is 13%;
Solvent: butanone is 20%;
Antifreezing agent: sorbierite is 3%;
Deionized water is mended to 100%.
Mixture is stirred in agitator, and the heating phase inversion promptly gets product.
The preparation of embodiment 10:25% rotenone+doractin water-dispersible granules, each composition weight proportioning is:
Rotenone is 5%;
Doractin is 20%;
Wetting agent: sodium lignin sulfonate is 10%;
Binding agent: polyvinyl acetate is 9%;
Disintegrant: urea is 15%;
Carrier: atlapulgite is mended to 100%;
Mixture is carried out granulation after the ultra-fine grinding, drying, screening promptly get product.
Embodiment 11:30% rotenone+more visit the preparation of rhzomorph water-dispersible granules, each composition weight proportioning is:
Rotenone is 10%;
More visiing rhzomorph is 20%;
Wetting agent: sodium lignin sulfonate is 10%;
Binding agent: polyvinyl acetate is 9%;
Disintegrant: urea is 10%;
Carrier: atlapulgite is mended to 100%;
Mixture is carried out granulation after the ultra-fine grinding, drying, screening promptly get product.
Embodiment 12: the preparation of 30% rotenone+doractin microcapsule formulations, and each composition weight proportioning is:
Rotenone is 7%;
Doractin is 23%;
Dispersant: polyvinyl alcohol is 10%;
Cyst material: gelatin is mended to 100%.
Each component was mixed high-speed stirred 2 hours, saltout and isolate polymer, the formation micro-capsule of heating, freeze drying promptly gets product again.
The foregoing description 1~12 mixed insecticide preparation sees Table 1 according to the conventional virulence experiment in laboratory and single agent agricultural chemicals comparing result.
Each Mixed Pharmacy of table 1 is to diamond-back moth Toxicity Determination result
Medicament |
Extension rate |
Lethality |
2.5% rotenone emulsifiable solution |
1000 |
32.6% |
5% emamectin suspending agent |
1500 |
56.4% |
5% according to general rhzomorph suspending agent |
1500 |
67.5% |
5% ivermectin suspending agent |
1500 |
56.3% |
The 5%allosamidin suspending agent |
1500 |
62.7% |
The 5%selamectin suspending agent |
1500 |
53.6% |
5% doractin suspending agent |
1500 |
61.8% |
5% more visits the rhzomorph suspending agent |
1500 |
53.8% |
0.05% rotenone+2.45% emamectin missible oil |
3000 |
80.3% |
0.3% rotenone+2.7% is according to general rhzomorph missible oil |
3000 |
83.4% |
2.95% rotenone+0.05% emamectin missible oil |
3000 |
84.1% |
70% rotenone+0.5% ivermectin wetting powder |
10000 |
100% |
0.5% rotenone+70% emamectin wetting powder |
10000 |
100% |
0.5% rotenone+9.5% emamectin wetting powder |
5000 |
93.4% |
10% rotenone+20%allosamidin oil-suspending agent |
8000 |
93.5% |
8% rotenone+12%allosamidin microemulsion |
6000 |
92.4% |
8% rotenone+10%selamectin microemulsion |
6000 |
94.6% |
5% rotenone+20% doractin water-dispersible granules |
8000 |
92.7% |
10% rotenone+20% is more visitd the rhzomorph water-dispersible granules |
8000 |
94.7% |
7% rotenone+23% doractin microcapsule formulations |
8000 |
97.1% |
Rotenone and the antibiotic insecticide synergy screening experiment that is mixed: single agent and mixture are made into the soup post processing diamond-back moth of variable concentrations, compare with clear water.Calculate the LC50 and the co-toxicity coefficient of medicament.Near 100 expression summation actions,, represent antagonisms as co-toxicity coefficient less than 100 greater than 100 expression synergistic effects.Result of the test sees Table 2.
Table 2 rotenone and allosamidin are mixed to diamond-back moth synergy screening test result
The medicament proportioning |
LC
50(μg/ml)
|
95% confidence limit |
Co-toxicity coefficient |
Rotenone |
39.86 |
24.71-64.29 |
0 |
allosamidin |
2.46 |
1.86-3.27 |
0 |
Rotenone: allosamidin (1: 100) |
2.26 |
1.49-3.43 |
110.05 |
Rotenone: allosamidin (1: 50) |
1.86 |
1.36-2.54 |
134.94 |
Rotenone: allosamidin (100: 1) |
34.28 |
21.28-55.22 |
101.08 |
Rotenone: allosamidin (50: 1) |
24.04 |
15.94-36.27 |
127.76 |
Rotenone: allosamidin (1: 1) |
2.75 |
1.95-3.86 |
168.89 |
Rotenone: allosamidin (1: 4) |
2.21 |
1.63-2.99 |
137.32 |
Rotenone: allosamidin (1: 10) |
2.11 |
1.39-3.18 |
127.90 |
Rotenone: allosamidin (1: 30) |
2.31 |
1.75-3.06 |
109.94 |
Table 3 rotenone and doractin are mixed to diamond-back moth synergy screening test result
The medicament proportioning |
LC
50(μg/ml)
|
95% confidence limit |
Co-toxicity coefficient |
Rotenone |
39.86 |
24.71-64.29 |
0 |
Doractin |
4.87 |
3.59-6.59 |
0 |
Rotenone: doractin (1: 100) |
5.04 |
3.25-7.81 |
102.44 |
Rotenone: doractin (1: 50) |
3.99 |
2.93-5.45 |
123.93 |
Rotenone: doractin (100: 1) |
32.33 |
20.75-50.38 |
115.09 |
Rotenone: doractin (50: 1) |
24.68 |
17.69-34.43 |
141.56 |
Rotenone: doractin (1: 1) |
7.19 |
5.34-9.69 |
120.55 |
Rotenone: doractin (1: 4) |
4.32 |
3.18-5.86 |
136.73 |
Rotenone: doractin (1: 10) |
4.37 |
2.90-6.57 |
121.13 |
Rotenone: doractin (1: 30) |
4.60 |
3.43-6.18 |
108.81 |
Table 4 rotenone and emamectin are mixed to diamond-back moth synergy screening test result
The medicament proportioning |
LC
50(μg/ml)
|
95% confidence limit |
Co-toxicity coefficient |
Rotenone |
39.86 |
24.71-64.29 |
0 |
Emamectin |
4.63 |
3.40-6.30 |
0 |
Rotenone: emamectin (1: 100) |
4.29 |
2.99-6.16 |
108.73 |
Rotenone: emamectin (1: 50) |
4.38 |
3.23-5.94 |
107.46 |
Rotenone: emamectin (100: 1) |
34.12 |
22.86-50.93 |
108.62 |
Rotenone: emamectin (50: 1) |
28.64 |
19.44-42.18 |
121.09 |
Rotenone: emamectin (1: 1) |
6.05 |
4.40-8.33 |
137.02 |
Rotenone: emamectin (1: 4) |
3.43 |
2.49-4.71 |
163.91 |
Rotenone: emamectin (1: 10) |
3.79 |
2.56-5.60 |
132.84 |
Rotenone: emamectin (1: 30) |
4.50 |
3.19-6.34 |
105.92 |
Table 5 rotenone be mixed to diamond-back moth synergy screening test result according to general rhzomorph
The medicament proportioning |
LC
50(μg/ml)
|
95% confidence limit |
Co-toxicity coefficient |
Rotenone |
39.86 |
24.71-64.29 |
0 |
According to general rhzomorph |
5.76 |
3.99-8.31 |
0 |
Rotenone: according to general rhzomorph (1: 100) |
4.57 |
3.15-6.64 |
127.08 |
Rotenone: according to general rhzomorph (1: 50) |
3.95 |
2.87-5.43 |
148.42 |
Rotenone: according to general rhzomorph (100: 1) |
32.33 |
20.75-50.38 |
116.46 |
Rotenone: according to general rhzomorph (50: 1) |
25.38 |
18.08-35.62 |
140.71 |
Rotenone: according to general rhzomorph (1: 1) |
5.50 |
3.91-7.73 |
183.19 |
Rotenone: according to general rhzomorph (1: 4) |
4.71 |
3.51-6.32 |
147.69 |
Rotenone: according to general rhzomorph (1: 10) |
3.64 |
2.72-4.87 |
171.82 |
Rotenone: according to general rhzomorph (1: 30) |
4.31 |
3.30-5.64 |
137.36 |
Table 6 rotenone and ivermectin are mixed to diamond-back moth synergy screening test result
The medicament proportioning |
LC
50(μg/ml)
|
95% confidence limit |
Co-toxicity coefficient |
Rotenone |
39.86 |
24.71-64.29 |
0 |
Ivermectin |
11.43 |
8.51-15.35 |
0 |
Rotenone: ivermectin (1: 100) |
9.75 |
6.35-14.96 |
118.14 |
Rotenone: ivermectin (1: 50) |
8.34 |
6.21-11.21 |
138.95 |
Rotenone: ivermectin (100: 1) |
31.05 |
20.22-47.68 |
125.28 |
Rotenone: ivermectin (50: 1) |
22.17 |
16.49-29.82 |
171.38 |
Rotenone: ivermectin (1: 1) |
10.98 |
8.32-14.50 |
161.77 |
Rotenone: ivermectin (1: 4) |
9.72 |
7.31-12.93 |
137.19 |
Rotenone: ivermectin (1: 10) |
9.49 |
6.41-14.05 |
128.85 |
Rotenone: ivermectin (1: 30) |
9.12 |
6.71-12.39 |
128.30 |
Table 7 rotenone with more visit rhzomorph and be mixed diamond-back moth synergy screening test result
The medicament proportioning |
LC
50(μg/ml)
|
95% confidence limit |
Co-toxicity coefficient |
Rotenone |
39.86 |
24.71-64.29 |
0 |
More visit rhzomorph |
1.39 |
1.01-1.92 |
0 |
Rotenone: more visit rhzomorph (1: 100) |
1.22 |
0.78-1.89 |
115.47 |
Rotenone: more visit rhzomorph (1: 50) |
1.05 |
0.76-1.45 |
134.59 |
Rotenone: more visit rhzomorph (100: 1) |
20.52 |
15.16-27.78 |
152.46 |
Rotenone: more visit rhzomorph (50: 1) |
15.11 |
10.52-21.71 |
171.01 |
Rotenone: more visit rhzomorph (1: 1) |
1.38 |
0.95-2.02 |
194.15 |
Rotenone: more visit rhzomorph (1: 4) |
12.74 |
0.80-1.53 |
155.29 |
Rotenone: more visit rhzomorph (1: 10) |
1.15 |
0.79-1.66 |
132.70 |
Rotenone: more visit rhzomorph (1: 30) |
1.28 |
0.94-1.76 |
111.76 |
Table 8 rotenone and selamectin are mixed to diamond-back moth synergy screening test result
The medicament proportioning |
LC
50(μg/ml)
|
95% confidence limit |
Co-toxicity coefficient |
Rotenone |
39.86 |
24.71-64.29 |
0 |
selamectin |
1.14 |
0.85-1.54 |
0 |
Rotenone: selamectin (1: 100) |
1.10 |
0.77-1.57 |
104.69 |
Rotenone: selamectin (1: 50) |
1.03 |
0.78-1.38 |
112.71 |
Rotenone: selamectin (100: 1) |
21.69 |
16.35-28.78 |
137.59 |
Rotenone: selamectin (50: 1) |
19.78 |
15.52-25.21 |
121.06 |
Rotenone: selamectin (1: 1) |
1.45 |
1.03-2.04 |
153.33 |
Rotenone: selamectin (1: 4) |
1.02 |
0.78-1.35 |
138.65 |
Rotenone: selamectin (1: 10) |
0.88 |
0.63-1.24 |
142.11 |
Rotenone: selamectin (1: 30) |
0.92 |
0.70-1.22 |
127.66 |
From table 2-8 as can be seen, rotenone and described antibiotic insecticide are mixed, and co-toxicity coefficient is all greater than 100, show that rotenone and these antibiotic insecticides rationally are mixed and have synergistic effect.
The field effect experiment of embodiment 13 rotenone and antibiotic insecticide mixed insecticide preparation
Test one: 2000 times 2.5% rotenone and emamectin missible oil to the diamondback moth larvae medication after the 1st, 3,7,14 day preventive effect be respectively 71.5%, 90.8%, 93.9%, 95.8%; 500 times of 2.5% rotenone emulsifiable solution to the diamondback moth larvae medication after the 1st, 3,7,14 day preventive effect be respectively 15.8%, 61.7%, 83.4%, 71.3%; 1000 times of 5% emamectin suspending agent to the diamondback moth larvae medication after the 1st, 3,7,14 day preventive effect be respectively 56.1%, 83.2%, 81.3%, 52.6%.
Test two: 5000 times 20% rotenone and allosamidin microemulsion to the diamondback moth larvae medication after the 1st, 3,7,14 day preventive effect be respectively 67.9%, 88.8%, 94.2%, 92.3%; 500 times of 2.5% rotenone emulsifiable solution to the diamondback moth larvae medication after the 1st, 3,7,14 day preventive effect be respectively 15.8%, 61.7%, 83.4%, 713%; 1000 times of 5%allosamidin suspending agents to the diamondback moth larvae medication after the 1st, 3,7,14 day preventive effect be respectively 43.3%, 57.5%, 68.8%, 53.1%.
Test three: 6000 times 25% rotenone and doractin water-dispersible granules to the diamondback moth larvae medication after the 1st, 3,7,14 day preventive effect be respectively 72.5%, 89.0%, 95.3%, 93.8%; 500 times of 2.5% rotenone emulsifiable solution to the diamondback moth larvae medication after the 1st, 3,7,14 day preventive effect be respectively 15.8%, 61.7%, 83.4%, 71.3%; 1000 times of 5% doractin suspending agent to the diamondback moth larvae medication after the 1st, 3,7,14 day preventive effect be respectively 53.6%, 61.1%, 71.9%, 50.3%.
Test four: 8000 times 70.5% rotenone and ivermectin wetting powder to the Spodoptera litura larvae medication after the 1st, 3,7,14 day preventive effect be respectively 77.4%, 89.6%, 98.3%, 93.4%; 500 times of 2.5% rotenone emulsifiable solution to the Spodoptera litura larvae medication after the 1st, 3,7,14 day preventive effect be respectively 25.3%, 51.4%, 73.2%, 61.38%; 1000 times of 5% ivermectin suspending agent to the Spodoptera litura larvae medication after the 1st, 3,7,14 day preventive effect be respectively 43.9%, 65.1%, 78.8%, 60.1%.
Contrast with former agricultural chemicals from the above-mentioned test of pesticide effectiveness explanation agricultural chemicals that the present invention was mixed, the control of insect is had remarkable synergistic effect and control efficiency.