CN100579985C

CN100579985C - Streptococcus agalactiae antigens I and II

Info

Publication number: CN100579985C
Application number: CN200480012219A
Authority: CN
Inventors: A·梅克; E·纳吉; M·汉纳; M·霍基; S·卡伦达; S·普拉斯托莫斯基
Original assignee: Intercell Austria AG
Current assignee: Valneva Austria GmbH
Priority date: 2003-05-07
Filing date: 2004-05-06
Publication date: 2010-01-13
Anticipated expiration: 2024-05-06
Also published as: CN1784419A

Abstract

本发明公开了编码超免疫血清反应性抗原或其片段和来自无乳链球菌的超免疫血清反应性抗原或其片段的分离的核酸分子、分离这样的抗原的方法及其特定用途。The present invention discloses isolated nucleic acid molecules encoding hyperimmune serum reactive antigens or fragments thereof and hyperimmune serum reactive antigens or fragments thereof from Streptococcus agalactiae, methods of isolating such antigens and specific uses thereof.

Description

Streptococcus agalactiae antigens I and II

The antigenic isolated nucleic acid molecule of streptococcus agalactiae (Streptococcus agalactiae) that the present invention relates to encode, described molecule are suitable for preventing and treat the preparation of the drug agents of the infectation of bacteria that streptococcus agalactiae causes

Streptococcus agalactiae is a gram positive bacterium, belongs to B group B streptococcus B (GBS) based on the haemolysis pattern on its blood agar.Described organism is encapsulated, and pod membrane is the important element that causes a disease.Pod membrane is antigenic, and forms the basis by serum group system GBS.Identified the distinct GBS serotype coldest days of the year end: Ia, Ib, II, III, IV, V, VI, VII and VIII.Show that most streptococcus agalactiae serotypes cause serious disease, estimate two serotype-III the most general and V-type-responsible most in the world invasive disease.Classification and serotype popular because of age group different with geographic range.In the U.S., GBS III type causes the diseases in infants more than 50%, and V-type causes about 40% non-pregnant adult diseases, and the Ia type causes about 1/3rd any patient groups' disease.

Streptococcus agalactiae is at the extreme age and those people of potential disease are arranged is reasons of very important human diseases.The B group B streptococcus B is the major cause of newborn infant's whole body and local infection.Since nineteen seventies, GBS is the neonatal main pathogens of the U.S..Infectation of bacteria can cause life-threatening disease, for example septicemia, pneumonia and meningitis.The survivor becomes hearing, study and permanent disbility with visual disabilities.The newborn infant has GBS to troop term or they to obtain described organism when the mother of (colonized) produces usually.In addition, GBS also is pregnant woman and chronic disease and older patient infection's a common cause, for example suffer those people (Balter of diabetes, malignant tumour, immunodeficiency diseases or the like, the summary of S etc., the Gram-positive pathogenic agent, editors such as Fischetti V.A., ASM Press 2000,154-160 page or leaf).

The 10-35% pregnant woman has GBS to troop, but asymptomatic.Yet because vertical danger of relaying, GBS troops very important.50-70% has the newborn infant that the women gave birth to that troops-be exactly all newborn infants of 5-15%-become in birth process to have GBS to troop.Trooping is the prerequisite of infection and disease.Most premature infants are because very low transmission of mother's antibody and jejune immunity system has the highest danger for the invasive disease.Carrying of GBS can be chronic, intermittence or temporary transient when conceived.The time length that very difficult evaluation is carried is because the women is in that screened mistake was once to the antenatal inspection from back three months.In some research prompting third trimester pregnancies and between the cluster state during childbirth dependency (～90%) is arranged.The serotype of isolate is pointed out continue the most general (Balter, the summary of S. etc., Gram-positive pathogenic agent, editors such as Fischetti V.A., ASM Press 2000, the 154-160 page or leaf) of same type.

Do not have preventative intervention, 1 to 2% all newborn infants and 15% have the newborn infant that the women gave a birth who seriously troops to form invasive disease (septicemia, pneumonia and/or meningitis).In the U.S., GBS infects influences 1-5 newborn infant/1000 a safety birth youngster.The annual GBS disease that about 17,000 cases take place of the U.S., 7,500 newborn infants that betide before the prevention.GBS is the most general reason of bacterium meningitis, causes～all cases of 40% this age group report.Total incidence in U.S. invasive GBS disease is 0.2-0.7/100,000.Slightly lower in Europe.The mortality ratio that the invasive disease does not prevent to intervene is 6%, the neonatal death of per 16 infection, survivor's lifelong disability of 20%.

The ratio that serious B group B streptococcus B infects is more a lot of than height in any other age group in the newborn infant.Yet serious B group B streptococcus B infects other age groups that occur in masculinity and femininity.Among the non-pregnant adult, the ratio of serious disease is in the scope of 4.1 to 7.2 cases of per 100,000 people.Invasive infection (bacterium enters the infection of part in the body that usually is not exposed to bacterium) average mortality is 8-10% for the adult of age in 18-64 year, is 15-25% 65 years old and above adult.Mortality ratio is younger adult and do not have the adult of other morbid state to hang down.The ratio of serious B group B streptococcus B disease increased along with the age.The mean age of case is about 60 years old among the non-pregnant adult.Most adult B group B streptococcus B diseases occur among the adult of serious disease state.These comprise: diabetes; Hepatic diseases; The apoplexy history; Cancer history; Or bedsore.The older, the ratio of serious B group B streptococcus B disease is more general with the bed inpatient in the occupant of nursing home.Among the non-pregnant adult usually after wound recently or accepting after some invasive hospital operation of for example operation B group B streptococcus B disease ({ Farley, M., 2001}; { Jackson, people such as L., 1995}; Www.cdc.gov/groupbstrep/).

The direct medical treatment cost of newborn child's illness is annual 200019400 U.S. dollars before the prevention, and GBS remains sizable economical load.

Making a definite diagnosis that streptococcus agalactiae infects generally depends on organism from the cotton excellent sampling of uterine cervix, blood or other separation in aseptic health site usually.Also can utilize and detect the antigenic test of capsular polysaccharide in the body fluid.

Penicillin G is that the treatment of the GBS case determined is selected.Recommend ten days treatment for microbemia, pneumonia and soft tissue infection, and recommend 2-3 week, for osteomyelitis 3-4 week for meningitis.

Set up prevention since 1994 in the North America, method is by carrying the GBS pregnant woman or differentiate risk factor by there is not culture when childbirth is admitted to hospital at conceived 35-37 week vagina and the cotton rod sampling of anal orifice and rectal intestine screening.Antibiotic therapy is to prevent the newborn child's illness of early onset thereof when producing in birth process for the women who prevents candidate.Method in this prevention of the U.S. made the GBS disease incidence rate be reduced to 0.4/1000 safety birth youngster from 1.7 between 1993 to 1999.Although the most newborn infant GBS diseases of prevention (penicillin G or Ampicillin Trihydrate) prevention in the time of passing through to produce, present available strategy is undesirable, especially for the infection of premature infant's late onset (＞7 days ages) and the prevention of disease.Always but some individuality becomes the intermittent carrier of male during for example in 32-35 week test feminine gender in childbirth, does not participate in, neglects or screen the date (32-35 week) preceding childbirth owing to some reasons are escaped the screening of carrying.

At last, antibiotic being extensive use of induced the anti-strain that occurs over time usually.Shown the stable per-cent (ref) that has increased the antibiotics resistance clinical isolates of penicillin and other antibiotic a large amount of uses (every 3-5 women accepts high-dose therapy).In addition, not so effective based on the biorefractive efficient of preventing for later stage outbreak disease, because it is at early onset thereof (dividing in 48 hours puerperiums).Another concern is and unresolved susceptible becomes crowd's prevention.

Shortage is carried the development that with the protective immunity element of disease enough understandings have hindered vaccine to antagonism GBS.Do not understand well and carry and autarcetic relation.In addition, do not define the immunologic mechanism that allows disease to take place well the carrier.Yet, the maternal serum level and the newborn infant GBS disease-related of pointing out pathogen specific antibody are arranged.The anti-capsular polysaccharide antibody horizontal of parent is relevant with invasive newborn child's illness negativity when determining childbirth very firmly.{ Campbell, people such as J., 2000}

Although the strain B group carbohydrate antigen all for GBS is very general, unfortunately, it does not have very strong immunogenicity and antibody not to protect the attack (challenge) that causes death in the experimental model yet.The GBS pod membrane of being made by polysaccharide itself is to have immunogenic and can induce protection antibody.Yet this protection is a type specific.Although the pod membrane specific antibody has shown very high protectiveness; these serotype specificity antibody of what concentration can resist disease and have provide protection still unclear; just, known that the avidity of opsonic activity and these antibody is the more crucial protection determinatives of specific concentration recently.

Having understood surface protein verifies human at the importance in the immunity of streptococcus agalactiae.Clearly all serotypes are expressed the surface protein that has with host immune defence related activity.α C protein, β C protein, Rib and Sip protein have feature description well on biochemistry and gene, also shown immunity and protectiveness ({ Michel, people such as J., 1991} in animal model; { Brodeur, people such as B., 2000}; { Larsson, people such as C., 1999}; Cheng, people such as Q., 2002}).These protein are as the subject matter of vaccine candidate their popular variability in the different clinical isolates of GBS seemingly.For example Rib protein is present among the III serotype GBS, but the V-type that accounts for the great part of whole world disease does not have.Some other surface protein is characterised in that it is immunogenic, but for differentiating that most GBS immunogenic proteins have only made limited system works.

Thereby the effective treatment that prevents or alleviate GBS to infect remains and needs.Vaccine can not only prevent the infection of GBS, also prevents more specifically or alleviates trooping of host tissue (especially at birth canal), thereby reduce the generation of propagating from the parent to embryo.Reduce the prevention that will cause invasive disease-pneumonia, microbemia, meningitis and septicemia among the newborn infant of carrying of the generation of acute infection and organism.Can show vaccine to most of streptococcus agalactiae strain cross-protections of causing the human infection for prevention or alleviate all other suis kinds, the infection that causes of A, C and G group just also is useful.

Vaccine can contain whole various antigen.Antigenic example is thin component, protein or their the simplest form peptide of organism that kill fully or attenuation, these organism/tissues.Antigen also can be with the form of glycosylated protein or peptide by immune system recognition, also can be or contains polysaccharide or lipid.Can use small peptide, because for example cytotoxic T cell (CTL) identification is connected in the antigen of small peptide form of common 8-11 amino acid long of major histocompatibility antigen (MHC).The B cell can be discerned and be short to 4-5 amino acid whose linear epitope and three-dimensional structure (conformational epitope).For the antigen specific immune reaction that obtains to continue, adjuvant need excite the immune cascade that comprises the immunity system all cells.Originally, adjuvant works, but is not limited to their patterns to the effect of so-called antigen presenting cell (APC).These cells run into antigen usually at first, are that immune effector cell is given in antigen presentation processing or unmodified afterwards.Also can relate to the intermediate cell type.Only there is suitable specific effector cell in the production immune response, to be activated.Adjuvant also can local other factors that keep antigen and inject altogether.In addition, adjuvant can play the chemical chemoattractant of other immunocyte, perhaps can be in the part and/or whole body play the immunity system stimulant.

Since later 1970s, vaccine development focuses on capsular polysaccharide, but does not still have product safely and effectively.Yet for exploitation with give baby and high-risk adult, it is one of most important that the vaccine of anti-streptococcus agalactiae is listed in.The conceptual phase that the vaccine of at present anti-described infection is only being developed.Achievement focus on use with or the capsular polysaccharide (CPS) that is not connected with protein as immunogen { Paoletti, people such as L., 2002}.Yet the use based on the vaccine of polysaccharide is opposed in some arguement.Polysaccharide induced IgG2 antibody, IgG2 antibody is to pass the embryo than IgG1 or the lower efficient of IgG3 antibody.For most susceptible newborn infants, the preborn, this is problem especially because 32-34 before week placenta antibody shift very low.Estimate that～10% childbirth occurred in before the 34th pregnant week.

Protein coupling vaccine is undoubtedly the modern weapons of a greatness of anti-GBS disease fight increase, but vaccine only contains the GBS serotype of limited quantity, give and enough ecological pressure, the disease that the NIS type is replaced remains real threat, especially in the zone that very high disease burden is arranged.The polysaccharide antigen that is used for active immne does not in addition provide immunological memory the mankind.CPS (for example combines with non-GBS related immune originality protein carrier, Toxoid,tetanus, choleratoxin B subunit or the like) be presented at that to induce the higher antibody concentration of vaccine inoculation person be favourable, but not being provided at, it does not recruit memory B and T cell in the real course of infection to support the pathogen specific B cell and the t cell epitope of effective host response.For the protein that can enoughly meet these standards replenishes the CPS vaccine, differentiate that conservative immunogenicity GBS specific surfaces protein is necessary.

All these not enough promptings need the Development of New Generation vaccine, and described vaccine is made up of the protein or derivatives thereof, and it is expressed by all strains under condition in vivo, and the ability of conditioning of inducing and/or neutralizing antibody is arranged the mankind.

Treatment based on passive antibody has very big potentiality.In order to prevent to attempt using the prepared product of human intravenously immunoglobulin (Ig) (IVIG).The latest developments of monoclonal antibody production technique provide the method that produces human antibodies reagent and introduce Antybody therapy again, and avoid the toxicity relevant with serum treatment.Immunoglobulin (Ig) is the anti-microbial protein of extremely various type, can be used to prevent and treat catching of appearance.It is effectively ({ Burnie, people such as J., the summary of 1998}) that Antybody therapy is resisted various microorganism.Giving to anti-GBS monoclonal antibody treatability each manifests the newborn infant of invasive disease or prophylactically gives low birthweight and newborn infant premature labor.

10 years in the past, proteinic immunogenicity of some GBS and protective capability have been described, in some animal models in order these to be probed into now based on the total proteinic developing vaccines of kind.Such protein is GBS surface protein Sip{Brodeur, people such as B., 2000}, rib, alpha protein and { Michel, people such as J., 1991}.

Be illustrated in and significantly cause lip-deep some protein of gram-positive organism or the enzyme that disease takes place to participate in the lysis that these pathogenic agent cause.Usually, these protein participated in directly interacting with host tissue or hiding bacterium surface to escape host defense mechanism { Navarre, people such as W., 1999}.Streptococcus agalactiae is also unexceptional in this.Some surface proteins have the feature of the very important virulence factor of curing the disease property of GBS ((people such as Fischetti V.A edit ASM Press2000,137-153 page or leaf for Paoletti in the Gram-positive pathogenic agent, people's such as L.C summary); Paoletti, people such as L., 2002}).If anti-these proteinic antibody can provide than polysaccharide better protection the mankind, they can provide novel source based on proteinic GBS vaccine to be connected in or to substitute more traditional capsular polysaccharide vaccine and use.Some as the above-described proteinic purposes of antigen that may vaccine and many additional candidate mainly by the generation of selecting based on the easy or obtainable chance of differentiating.Differentiate that in comprehensive more mode the streptococcus agalactiae related antigen has demand.

The present inventor has developed the method (WO02/059148) of especially differentiating, separating and produce the hyper-immuneserum reactive antigen from specific pathogen from streptococcus aureus (Staphylococcus aureus) with staphylococcus epidermidis (Staphylococcusepidermidis).Yet, suppose the different of pathogenic function of biological nature and genetic background, streptococcus agalactiae and aureus strain are completely different.Importantly, in order to differentiate the antigen of streptococcus agalactiae, the selection of serum is different from that is applied to the streptococcus aureus screening.For that purpose, collected the antibody sources of human four main types.At first, GBS uterine cervix and anal orifice and rectal intestine carry the negative healthy pregnant woman of test.This donor group has been represented the most important source of antibody.Except their serum sample, the human cervical cancer 1 secretory product of collecting with the uterine cervix gauze that contains secretory IgA (sIgA) also is used for antigenic discriminating and affirmation.The chief value of this gleanings is that sIgA can be considered to the main immune effector molecule on the mucomembranous surface.Secondly, the healthy pregnant woman who has GBS to troop, its newborn infant still keep not having GBS (although having used antibiotic prophylaxis).The 3rd, do not have clinical disease less than 45 years old adult.The 4th, inmature individual, the child between 5 to 10 months does not obtain the GBS specific antibody after they lose maternal antibody and owing to the shortage of GBS disease.

In order to select relevant serum source, carried out measuring a series of ELISA and the immunodotting experiment of anti-streptococcus agalactiae IgG and IgA antibody horizontal with bacterial lysate and culture supernatant protein.Serum from high titre carrier and non-carrier is included in based on genomic antigen discriminating.This method of selecting human serum is very different with the method that streptococcus aureus is used basically, here carry or non-carrier state not relevant with antibody horizontal.

The present invention has used the high-throughput genome method to differentiate the pathogen specific protein that the expression in vivo of inducing the human antibodies ability is arranged in infection and clustering procedure.

The genome itself of two bacterium kind-streptococcus agalactiaes and streptococcus aureus has shown many important different.The genome of streptococcus agalactiae contains about 2.2Mb, and streptococcus aureus has 2.85Mb.They have 35.7% and 33% average GC content respectively, about 30% to 45% encoding gene difference between two pathogenic agent.In addition, need different growth conditions and substratum for two bacterial species of propagation.The tabulation of the most important diseases that two pathogenic agent cause shows below.Streptococcus aureus mainly causes the opportunistic infection of hospital: the wound of pustulosis, folliculitis, abscess, furuncle, infection, endocarditis, meningitis, septic arthritis, pneumonia, osteomyelitis, skin scald syndromes (SSS), toxic shock syndrome.Streptococcus agalactiae mainly causes infection of newborn and the elderly's disease, for example microbemia, septicemia, wound infection, osteomyelitis and meningitis.

Be appointed as the complete genome sequence of isolate of the streptococcus agalactiae pod membrane serotype III of NEM316 (ATCC 12403) and measure (GenBank number of registration AL732656 by the order-checking of air gun at random strategy; See www.tigr.org/tigrscripts/CMR2/CMRHomePage.spl) { Glaser, people such as P., 2002}.

A potential difficult problem of the present invention provides the method that for example anti-streptococcus agalactiae of exploitation infects the medicament of vaccine.More especially, a described difficult problem provides a cover effective, relevant and the comprehensive nucleic acid molecule or the hyper-immuneserum reactive antigen from streptococcus agalactiae that can be used to produce described medicament.

So, the invention provides the high immune response sero-reaction antigen of coding or its segmental isolated nucleic acid molecule, comprise following nucleotide sequence, be selected from

A) be selected from Seq ID No 14,90, the nucleic acid molecule of 157-216 has the nucleic acid molecule of 70% sequence homogeny at least,

B) be complementary to the nucleic acid molecule of nucleic acid molecule a),

C) comprise a) or b) the nucleic acid molecule of at least 15 continuous bases of nucleic acid molecule

D) under stringent hybridization condition, anneal in a), b) or the nucleic acid molecule of c) nucleic acid molecule

E) because the degeneracy of genetic code, otherwise will hybridize in a), b), c) or d) in the nucleic acid molecule of the nucleic acid molecule that defines.

According to the preferred embodiments of the invention, the sequence homogeny is at least 80%, preferably at least 95%, especially 100%.

In addition, the invention provides coding hyper-immuneserum reactive antigen or its segmental isolated nucleic acid molecule, comprise following nucleotide sequence, be selected from

A) be selected from

Seq ID No

1,3,5-13,15,18-25,27-31,33-36,39-68,70-85,92-100,103-126,128-145,147,149-156,217, the nucleic acid molecule of 435-448 and 463-474 has 96% or be higher than 96% at least, preferably at least 98%, the nucleic acid molecule of 100% sequence homogeny especially

B) be complementary to the nucleic acid molecule of nucleic acid molecule a),

According to another aspect, the invention provides coding hyper-immuneserum reactive antigen or its segmental isolated nucleic acid molecule, comprise following nucleotide sequence, be selected from

A) be selected from Seq ID No 32,86,91,101,127 nucleic acid molecule has 98% or be higher than 98%, the nucleic acid molecule of 100% sequence homogeny especially,

B) be complementary to the nucleic acid molecule of nucleic acid molecule a),

C) because the degeneracy of genetic code, otherwise will hybridize in a), b), c) or d) in the nucleic acid molecule of the nucleic acid molecule that defines.

Preferably, nucleic acid molecule is DNA or RNA.

According to the preferred embodiment of the invention, nucleic acid molecule is from the especially genomic dna separation of streptococcus agalactiae genomic dna.

According to the present invention, provide the carrier that comprises according to arbitrary nucleic acid molecule of the present invention.

In preferred embodiments, carrier is segmental recombinant expressed through transforming to be suitable for according to the hyper-immuneserum reactive antigen of nucleic acid molecule encoding of the present invention or its.

The present invention also provides the host cell that comprises support according to the present invention.

According to having the present invention further provides the hyper-immuneserum reactive antigen that comprises according to the aminoacid sequence of nucleic acid molecule encoding of the present invention on the other hand.

In the embodiment preferred, aminoacid sequence (polypeptide) is selected from Seq ID No 231,307,374-433.

Aminoacid sequence in another preferred embodiment (polypeptide) is selected from Seq ID No 218,220,222-230,232,235-242,244-248,250-253,256-285,287-302,309-317,320-343,345-362,364,366-373,434,449-462 and 475-486.

In the further preferred embodiment, aminoacid sequence (polypeptide) is selected from Seq ID No 249,303,308,318,344.

The present invention also provides and has produced according to streptococcus agalactiae hyper-immuneserum reactive antigen of the present invention or its segmental method, is included in the appropriate expression system and expresses according to one or more nucleic acid molecule of the present invention.

In addition, the invention provides and produce the method for expressing according to streptococcus agalactiae hyper-immuneserum reactive antigen of the present invention or its segmental cell, comprise with support according to the present invention transforming or the transfection proper host cell.

According to the invention provides the especially pharmaceutical composition of vaccine, it comprises as the hyper-immuneserum reactive antigen of the present invention's definition or its fragment or as the nucleic acid molecule that defines of the present invention.

In the preferred embodiment, pharmaceutical composition has further comprised the immunostimulation material, preferably is selected from polycationic polymer, the immunostimulation deoxynucleotide (ODN) of polycation peptide especially, the Neurologically-active compounds that contains especially peptide, the especially human growth hormone of at least two LysLeuLys groups of KLKL5KLK, alum (alumn), Fu Shi fully or Freund or their combination.

In the embodiment that is more preferably, the immunostimulation material is the combination of polycationic polymer and immunostimulation deoxynucleotide or peptide and the immunostimulation deoxynucleotide that contains at least two LysLeuLys groups.

In the also preferred embodiment, polycationic polymer is polycation peptide, especially poly arginine.

According to the present invention, provide according to nucleic acid of the present invention or according to hyper-immuneserum reactive antigen of the present invention or its fragment to be used for the purposes of the production of pharmaceutical preparations the production of the vaccine that infects in particular for anti-streptococcus agalactiae.

Herewith, also provide and be incorporated at least according to the antibody of the segmental selectivity part of hyper-immuneserum reactive antigen of the present invention or its or its significant part at least.

Antibody is monoclonal antibody in the embodiment preferred.

The significant part of antibody comprises the Fab fragment in another embodiment preferred.

Antibody is chimeric antibody in the further preferred embodiment.

Also antibody is humanized antibody in the embodiment preferred.

The present invention also provides the hybridoma cell line that produces according to antibody of the present invention.

In addition, the invention provides the method for generation, be characterised in that following step according to antibody of the present invention:

By give inhuman animal as defining among the present invention the hyper-immuneserum reactive antigen or its fragment start as described in the immune response of animal,

Shift out the body fluid that contains antibody from described animal, and

By being further purified the body fluid generation antibody that step process contains described antibody.

Correspondingly, the present invention also provides the method for a kind of generation according to antibody of the present invention, is characterised in that following step:

Shift out spleen or splenocyte from described animal,

Produce the hybridoma of described spleen or splenocyte

Select and the clone is specific to described hyper-immuneserum reactive antigen or its segmental hybridoma,

The cultivation of the hybridoma by described clone and optionally be further purified step and produce antibody.

According to the medicaments preparation that top method provides or the antibody produced can be used for the treatment of or prevent streptococcus agalactiae to infect.

According on the other hand, the invention provides combination according to hyper-immuneserum reactive antigen of the present invention or its segmental antagonist.

Described can the discriminating by the method that comprises following steps in conjunction with hyper-immuneserum reactive antigen according to the present invention or its segmental antagonist:

A) allowing under candidate antagonist and described hyper-immuneserum reactive antigen or its fragment bonded condition according to hyper-immuneserum reactive antigen of the present invention or its fragment isolating or immobilized, can provide the component that responds to candidate antagonist and described hyper-immuneserum reactive antigen or its fragment bonded detectable signal in the presence of, contact with described candidate antagonist; And

B) detection responds to the existence of antagonist and hyper-immuneserum reactive antigen or its fragment bonded signal or does not exist.

Can reduce or suppress can differentiate by the method that comprises following steps according to hyper-immuneserum reactive antigen of the present invention or its fragment active antagonist of reacting to each other of companion that reacts to each other with it:

A) provide according to hyper-immuneserum reactive antigen of the present invention or its high immune fragment,

B) to described hyper-immuneserum reactive antigen or its fragment, provide the companion that reacts to each other, especially according to antibody of the present invention,

C) allow the reacting to each other of described hyper-immuneserum reactive antigen or its fragment and the described companion of reacting to each other with the formation mixture that reacts to each other,

D) provide candidate antagonist,

E) allow candidate antagonist and react to each other competing reaction takes place between the mixture,

F) measure the activity that reacts to each other whether candidate antagonist suppresses or reduce hyper-immuneserum reactive antigen or its fragment and the companion that reacts to each other.

Can be used for described hyper-immuneserum reactive antigen or its segmental companion's of reacting to each other separation and/or purifying and/or discriminating according to hyper-immuneserum reactive antigen of the present invention or its fragment.

The present invention also provides the method for the in-vitro diagnosis disease relevant with hyper-immuneserum reactive antigen according to the present invention or its segmental expression, comprise encode according to the present invention existence of described hyper-immuneserum reactive antigen or its segmental nucleotide sequence of mensuration, or according to hyper-immuneserum reactive antigen of the present invention or its segmental existence.

The present invention also provides the method for the infectation of bacteria in-vitro diagnosis that streptococcus agalactiae especially infects, and comprises encode according to the present invention existence of described hyper-immuneserum reactive antigen or its segmental nucleotide sequence or according to hyper-immuneserum reactive antigen of the present invention or its segmental existence of analysis.

In addition, the invention provides that it is incorporated into described hyper-immuneserum reactive antigen or its segmental peptide in order to generation according to hyper-immuneserum reactive antigen of the present invention or its segmental purposes, wherein said peptide is anti-caline (anticaline).

The present invention also provides according to hyper-immuneserum reactive antigen of the present invention or its segmental purposes, and it is in order to produce functional nucleic acid, and wherein said functional nucleic acid is selected from fit (aptamer) and specular surface body (spiegelmers).

Also can be used for the production of functional Yeast Nucleic Acid according to nucleic acid molecule of the present invention, wherein said functional Yeast Nucleic Acid is selected from ribozyme, antisense nucleic acid and siRNA.

The present invention has advantageously provided to use from a plurality of human plasmas and has merged the antibody preparations of things and derive from the genomic surface expression of streptococcus agalactiae library, a cover of differentiating out from streptococcus agalactiae effectively, hyper-immuneserum reactive antigen or its fragment relevant and comprehensively isolated nucleic acid molecule and their codings.Thereby the present invention has satisfied the extensive needs of streptococcus agalactiae antigen, vaccine, diagnosis and product useful in the process of the compound that preparation antibody and the effective antagonism of discriminating streptococcus agalactiae infect.

Effective vaccine should also can induce the high-affinity of anti-streptococcus agalactiae cell surface component, the protein that enriches antibody or polypeptide to form by what all strains were expressed.For opsonization, antibody should be IgG1 and/or IgG3, for the neutralization of adhesion and detoxifying function, is any IgG hypotype and IgA.Chemically defined vaccine must be better than whole-cell vaccines clearly; because can be removed, and induce protection antibody and/or protect immunoreactive individual proteins can be selected to come out with human tissue cross reaction or the component that suppresses the streptococcus agalactiae of opsonization.

The method that the present invention adopts is based on the interaction of the antibody that exists in GBS protein or peptide and the human serum.Expression in vivo that human immune system is that produce and anti-streptococcus agalactiae antibody that exist in human serum is antigen protein and their immunogenic indication.In addition, handle antigen protein in second and the third round screening that the serum with individual selection or generation carries out, described antigen protein is to use the merging thing of preliminary election serum to differentiate by the bacterium surface displaying expression library.Thereby, the invention provides effective, relevant, the comprehensive GBS antigen of a cover that especially prevents the vaccine of streptococcus agalactiae infection as pharmaceutical composition.

In differentiating according to of the present invention one a complete set of antigenic antigen evaluator, the body fluid that contains antibody (antibody merging thing) that merges thing or blood plasma fraction or other merging with some serum has screened at least two different bacterium surface expression libraries.Antibody merges the serum gleanings that thing has derived from after tested anti-streptococcus agalactiae antigen compound, for example whole cell extracts and culture supernatant protein.Preferably, use three distinct serum gleanings, derive from ad 1. healthy pregnant woman, it is negative that uterine cervix and anal orifice and rectal intestine GBS carry test, ad 2. healthy pregnant woman, uterine cervix and/or anal orifice and rectal intestine GBS carry the test positive, and its newborn infant does not still have GBS (although having used the antibiont prevention), and ad 3. is lower than the adult who did not have clinical disease in 45 years old.For consider hyperimmune and thereby in the screening method that the present invention adopts, be correlated with, serum must with a plurality of GBS specific antigenss reactions.

The possible antigenic expression of institute be should allow as the expression library that uses among the present invention, all excretory of streptococcus agalactiae and surface protein for example are derived from.{ Georgiou, G., 1997} are represented by the reorganization library the host bacterium of oblatio streptococcus agalactiae one (always) the cover expression of peptides sequence of two outer membrane proteins of selecting (LamB and FhuA) of host bacterium film in the bacterium surface displaying library; { Etz, people such as H., 2001}.Using the advantage in recombinant expressed library is that the hyper-immuneserum reactive antigen differentiated can be immediately produced by the expression of the clone's of expression hyper-immuneserum reactive antigen screening and that select encoding sequence, does not have further recombinant DNA technology or necessary clone's step.

Further, analyze a complete set of antigen of differentiating by the program of describing according to the present invention with the screening of one or more extra wheel.So, used the antibody of being differentiated the anti-selected peptide that immunogenic individual antibody preparations or generation are arranged.According to preferred embodiment, be used for the second individual antibody preparations of taking turns screening and derive from pregnant woman and the pregnant adult of non-that the demonstration antibody titers is higher than certain minimum level, for example antibody titers is higher than test person (patient or the healthy individual) serum of 80 percentage ratios, preferably be higher than 90 percentage ratios, especially be higher than 95 percentage ratios.Taking turns screening second uses the individual antibody preparations of high like this titre to allow hyper-immuneserum reactive antigen and its fragment very selectively differentiating from streptococcus agalactiae.

Comprehensively after the screening process, take turns second by a series of ELISA and Western spot test and to have tested the antigen protein selected in the screening to estimate their immunogenicities to a large amount of human serum gleanings (minimum～150 health and patients serum), express attach most importance to histone matter or external translation product under the situation that described antigen protein can not be expressed in prokaryotic expression system, perhaps the antigenic peptide of Jian Bieing (the synthetic generation).

Individual antibody preparations (serum that it is also selected) allows the selectivity of the most promising material standed for of all hyper-immuneserum reactive antigen from all material standed fors likely of the first round to differentiate it is important.So preferably at least 10 individual antibody preparations (promptly from least 10 antibody preparations (for example serum) that suffered the Different Individual of selected pathogenic infection) should be used for taking turns these antigens of screening discriminating second.Certainly, also can use to be less than 10 individual prepared products, yet the individual antibody preparations of the selectivity of step and low quantity may not be best.On the other hand, if given hyper-immuneserum reactive antigen (or its antigen fragment) is by at least 10 individual antibody preparations, preferably at least 30, especially at least 50 individual antibody preparations identifications, the discriminating of hyper-immuneserum reactive antigen also has enough selectivity to correct discriminating so.Can certainly with individual prepared product as much as possible (for example with more than 100 or even with more than 1,000) test hyper-immuneserum reaction.

So the relevant portion of the hyper-immuneserum reaginic antibody prepared product of the method according to this invention should be preferably at least 10, more preferably at least 30, at least 50 individual antibody preparations especially.Perhaps (or jointly) hyper-immuneserum reactive antigen preferably also available second take turns use in the screening at least 20%, preferably at least 30%, especially the individual antibody preparations of all of at least 40% is differentiated.

According to the preferred embodiments of the invention, be used for second and take turns the individual antibody preparations of screening from titre by their anti-streptococcus agalactiaes of the serum of its preparation (prepared product of anti-this pathogenic agent for example, lysate for example, cell-wall component and recombinant protein) select.Preferably, when full organism among the ELISA (total lysate or total cell) during as antigen, select total IgA titre and be higher than that 300U especially is higher than 500U's and/or the IgG titre is higher than 5,000U especially is higher than 10, (U=unit is from given dilution OD for 000U _405nmReading calculating) some serum.

Streptococcus that human immune system produces and the antibody that is present in the human serum are the expression in vivo of antigen protein and their immunogenic indication.The identification of the linear epitope of serum antibody identification can be based on the sequence short as 4-5 amino acid.Certainly, it is not to mean that necessarily these small peptides can induce given antibody in the body.For that reason, the epi-position of definition, polypeptide and protein are tested them further and are induced the ability of selecting proteinic antibody in the antibody in animal (mainly being mouse).

Preferred antigen is positioned at cell surface or is secreted, but and thereby born of the same parents outer approaching.The antibody of expectation anti-cell wall-held protein matter plays a plurality of effects: suppress to adhere to, disturb acquisition, the inhibition immune evasion of nutrition and promote phagocytosis { Hornef, people such as M., 2002}.The secretion inhibitor protein antibody is useful to them as the neutralization of the function of toxin or virulence component.It also is known that bacterium connects each other by secretory protein.Among anti-these protein and antibody will disturb between the hammer mushroom or suis in the promotion contact (cross talk) of growing.Analysis of biological information (signal sequence, cell walls signal for locating, membrane spaning domain) proves that for estimating cell surface location or secretion be very useful.The antibody that laboratory method includes corresponding epi-position and protein separate from human serum, and the generation of the immune serum of (many) peptides that the screening of antibacterium surface display is selected in the mouse.These serum are used for the third round screening as the reagent in the following test then: the promotion of the cell surface dyeing (FACS or microscope) of the streptococcus agalactiae that grows under different condition, the mensuration (toxin, adhesion) of neutralising capacity and opsonization and phagocytosis (external phagocytosis test).

For described purpose, bacteria Escherichia coli bacterium colony direct injection is advanced mouse, collect immune serum and test function conditioning or neutralizing antibody in relevant in vitro tests.Perhaps, can use peptide or protein as substrate from people or mice serum purification specificity antibody.

Mainly rely on conditioning phagocytosis property at the host defense of streptococcus agalactiae and kill mechanism.By vaccine inoculation induce high-affinity conditioning and in and the antibody of type help innate immune system to remove bacterium and toxin.This makes the method according to this invention become the best instrument that the GBS antigen protein is differentiated.

The film of skin and mucous membrane is the barrier that powerful streptococcus is invaded.Yet in case skin or adherent film are destroyed, the epidemic prevention of the first road non-habitual cell begins its cooperation effect by complement and phagocyte especially polymorphonuclear leukocyte (PMN).These cells can be taken as the foundation stone of removing the invasion bacterium.Because streptococcus agalactiae is main extracellular pathogenic agent, main streptococcus adaptation reaction is from immune body fluid approach, and by three main mechanism mediations: the promotion of opsonization, the neutralization of toxin and adherent inhibition.Believe that opsonization is even more important because of its requirement to effective phagocytosis.For effective opsonization, for the identification of PMN by the acceptor of IgG molecule Fc fragment or activated C3b, antibody and complement factor bag quilt must be used in the microorganism surface.After the opsonization, suis is engulfed and is killed.The antibody that is incorporated into the lip-deep specific antigens of bacterial cell works to be attached to the part of PMN and promotes phagocytosis.Expectation is incorporated in the very the same antibody of adhesin and other cell surface proteins and adhesion and prevention are trooped.Antigenic selection provided by the invention thereby be very suitable for differentiating those antigens that will cause anti-infective protection among the anti-animal model or the mankind.

According to the antigen discrimination method that uses herein, the present invention shockingly provides a comprehensive streptococcus agalactiae novel nucleic acids of cover especially as described below and novel hyper-immuneserum reactive antigen and its fragment.According to an aspect, the invention particularly relates to the nucleotide sequence of coding hyper-immuneserum reactive antigen, described sequence is listed in Seq ID No:1-217 in the sequence list, 435-448 and 463-474, and the respective coding aminoacid sequence of representing the hyper-immuneserum reactive antigen is listed in Seq ID No 218-434,449-462 and 475-486 in the sequence list.

In the preferred embodiment of the invention, provide its total length to show and Seq ID No 14,90, the nucleic acid molecule of nucleotide sequence 70% homogeny that 157-216 lists.Most preferably comprise its total length and Seq ID No 14,90, the nucleic acid in the zone of nucleic acid molecule at least 80% that 157-216 lists or at least 85% homogeny.In this, nucleic acid molecule is with regard to its total length and Seq ID No 14,90, the nucleic acid molecule at least 90% that 157-216 lists, 91%, 92%, 93%, 94%, 95% or 96% identical be especially preferred.In addition, those that at least 97% homogeny arranged are preferred, have at least 98% and at least 99% those be especially highly preferred, have at least 99% or 99.5% to be more preferably, it is especially preferred that 100% homogeny is arranged.In addition, preferred embodiment is to encode to have kept basically and Seq ID No 14,90 identical biological function of the mature polypeptide of the described nucleic acid encoding that 157-216 lists or active hyper-immuneserum reactive antigen or its segmental nucleic acid on this aspect.

As known in the art and herein the homogeny that uses is two or more peptide sequences measured by comparative sequences or the relation between two or more polynucleotide sequences.In this area, homogeny also refers to the relevant degree of sequence between polypeptide or the polynucleotide sequence, and just the coupling between the sequence string (strings) as under in this situation can being is measured.Can calculate homogeny at an easy rate.Though have many methods to measure homogeny between two polynucleotides or two peptide sequences, described term is well-known (for example sequential analysis in the molecular biology, von Heinje, G., Academic Press, 1987) for the technician.Design is measured the preferred method of homogeny with coupling maximum between the sequence of testing.Coding is measured the method for homogeny in computer program.The preferred computer program technic of measuring homogeny between two sequences includes but not limited to GCG routine package { Devereux, people such as J., 1984}, BLASTP, BLASTN and FASTA{Altschul, people such as S., 1990}.

According to a further aspect in the invention, provide and represented and

Seq ID No

1,3,5-13,15,18-25,27-31,33-36,39-68,70-85,92-100,103-126,128-145,147,149-156,217, the nucleotide sequence that 435-448 and 463-474 list has 96% or be higher than 96%, especially the nucleic acid molecule of 100% sequence homogeny.

According to a further aspect in the invention, provide with nucleic acid molecule to have 98% or be higher than 98%, the nucleic acid molecule of 100% sequence homogeny especially, described nucleic acid molecule is identical with Seq ID No 32,86,91,101,127 nucleotide sequences of listing.

But nucleic acid molecule according to the present invention also is complementary to above-mentioned first nucleic acid molecule of selecting the nucleic acid of scheme fully at least basically as second scheme of selecting fully.As using ground herein, complementation is meant that nucleic acid chains is base pairing via Watson-Crick base pairing and second nucleic acid chains.As use herein ground basically complementary be meant that base pairing does not occur in all bases of each chain and leaves the not pairing or the mismatched bases of some amount or per-cent.The per-cent of correct pairing base preferably at least 70%, more preferably 80%, even more preferably 90% and any per-cent of most preferably being higher than 90%.The per-cent of noting 70% pairing base has been considered to homology, and it is strict that the hybridization with this degree pairing base pair is considered to.The hybridization conditions of this strict hybridization can obtain from the modern testing program of molecular biology (John Wiley and Sons, Inc., 1987).More especially, hybridization conditions is as follows:

For example at 5x SSPE, 5x Denhardt ' s reagent, 0.1%SDS, among the DNA that 100g/mL shears 68 ℃ of hybridization of carrying out

0.2xSSC the medium strictness at 42 ℃ among the 0.1%SDS is cleaned

0.1xSSC the high strictness at 68 ℃ among the 0.1%SDS is cleaned

There is the genomic dna of 50%GC content to have about 96 ℃ T _MMispairing for 1%, T _MReduce about 1 ℃.

In addition, any further hybridization conditions described herein also can be used in principle.

Certainly, coding comprises any disclosed given encoding sequence with all nucleotide sequence molecules of those identical peptide molecules of differentiating as the present invention, because the degeneracy of genetic code can directly be used clearly to measure all possible nucleic acid molecule of the given peptide molecule of coding, even the quantity of the nucleic acid molecule of degeneracy can be very high like this.This also can be applicable to the fragment of given polypeptide, as long as this puts the polypeptide that the above fragment coding is suitable as for example conduct active or passive vaccine in immunization.

Nucleic acid molecule according to the present invention also can be to have comprised the nucleic acid of extension of selecting at least 15 bases of nucleic acid molecule of scheme according to the nucleic acid molecule first and second according to the present invention of summing up above fully as the 3rd scheme of selecting fully.Preferably, base has formed the extension of base successive.Yet two or more groups that described extension is separated by many bases are formed, also within the scope of the invention.

At least 20 of the preferably disclosed from here sequences of this nucleic acid, even more preferably at least 30, especially at least 50 continuous based compositions.(for example as (PCR) primer, probe, (for example on (DNA) chip) capture molecules or the like) optimizes suitable length at an easy rate because the plan area of purposes.The having of continuous at least 15 base portions, especially tables 2 that preferred nucleic acid molecule contains the immunogenicity aminoacid sequence of listing in one or more tables 1 and 2 of inferring is higher than 10 fens, preferably is higher than 20 fens, especially has to be higher than 25 minutes sequence.Especially preferred is the nucleic acid that contains the dna sequence dna sequential portion of any sequence in the sequence solution that should use, described nucleic acid and disclosed streptococcus agalactiae strain NEM316 (ATCC 12403) genome ({ Glaser, people such as P., 2002}; The GenBank number of landing AL732656) and/or any other disclosed streptococcus agalactiae genome sequence or its part, the especially 2603V/R of serotype V (A909) strain Tettelin, people such as H., 2002}); The GenBank number of landing AE009948), shown one or more, preferably more than 2, especially more than 5 nucleic acid residues inequality.Especially preferred nucleic acid residue inequality is the residue that causes the different aminoacids residue.Preferably, nucleic acid sequence encoding and disclosed streptococcus agalactiae counterpart above-mentioned have at least 1, and preferably at least 2, the polypeptide of at least 3 different aminoacids residues preferably.Isolated polypeptide also is preferred like this, and described polypeptide is the fragment of the protein (or holoprotein) herein for example mentioned in the sequence list, has at least 6,7 or 8 amino-acid residues and by these nucleic acid encodings.

Nucleic acid molecule according to the present invention is also annealed under stringent hybridization condition in according to first, second and the 3rd nucleic acid molecule of selecting any nucleic acid of the present invention of scheme fully of summing up above as the 4th scheme of selecting fully.Stringent hybridization condition generally be described herein those.

At last, nucleic acid molecule according to the present invention is selected scheme also because the degeneracy of genetic code otherwise can hybridize according to what sum up above and the first, second, third and the 4th select the nucleic acid molecule of any nucleic acid molecule of any nucleic acid molecule according to the present invention of scheme fully fully as the 5th.This nucleic acid molecule refers to nucleic acid encoding preferably according to the present invention according to hyper-immuneserum reactive antigen of the present invention or its segmental fact.This nucleic acid is for the detection according to nucleic acid molecule of the present invention, and thereby diagnosis of each microorganism of for example streptococcus agalactiae and any disease or the morbid state relevant with this microorganism is particularly useful.Preferably, hybridization will take place under the stringent condition that the 4th scheme of selecting is fully described as described above or carry out.

Referring to any nucleic acid molecule or dna molecules herein as the nucleic acid molecule that uses, can be the RNA of non-modification or the RNA or the DNA of DNA or modification.Thereby, for example, other among refer to list and double-stranded DNA as the nucleic acid molecule that uses herein, the DNA of the mixture of list and double-stranded RNA, the RNA of the mixture of list and double-stranded region, comprising can be strand or more double-stranded or three chains or the DNA of list and double-stranded region mixture and the hybrid molecule of RNA.In addition, refer to the three chain zones that comprise RNA or DNA or RNA and DNA as the nucleic acid molecule that uses herein.Qu Yu chain can be from identical molecule or from differing molecular like this.These zones can comprise all one or more molecules, but more generally only relate to the zone of some molecules.The molecule in a triple helical zone is oligonucleotide normally.As using ground herein, the term nucleic acid molecule comprises DNA or the RNA that contains one or more modified bases as described above.Thereby, have for the DNA of stability or thereby the skeleton modified former or RNA for other be " nucleic acid molecule " of term indication herein.In addition, comprise the unusual base of the inosine for example or for example DNA of the modified base of tritylation base or RNA (only mentioning two examples) are nucleic acid molecule as the term that uses herein.Understanding is carried out very multiple modification to the DNA and the RNA of the known a lot of useful purposes of capable field technique personnel.The nucleic acid molecule of chemistry, enzyme or metabolism modified forms as the term nucleic acid molecule that herein adopts comprises, and virus and comprising the DNA and the RNA of the chemical species of the cell characteristic of simple and complex cell.The term nucleic acid molecule also comprises the short nucleic acid molecule that is commonly referred to as oligonucleotide." polynucleotide " and " nucleic acid " or " nucleic acid molecule " are used alternatingly usually herein.

Nucleic acid molecule provided by the invention also comprises the fragment of numerous uniquenesses, and the sequence of nucleic acid molecules of listing in the sequence list than streptococcus agalactiae coding region is longer and shorter, can produce by the standard cloning process.In order to be unique, fragment must have enough sizes that it can be distinguished from other known nucleotide sequence, and is the easiest of the nucleotide sequence comparison in the Computer Database of any streptococcus agalactiae fragment of selecting and for example GenBank is measured.

In addition, can modify nucleic acid molecule and polypeptide that the present invention comprises.For example, can not influence the nucleotide substitution of the polypeptide of nucleic acid encoding, thereby any nucleic acid molecule of coding hyper-immuneserum reactive antigen or its fragment coding is also contained in the present invention.

In addition, use the standard technique of standard clone technology for example coding hyper-immuneserum reactive antigen provided by the invention or its segmental any nucleic acid molecule functionally can be connected in any desired adjusting sequence, no matter be that streptococcus agalactiae is regulated the heterology encoding sequence that sequence or heterology are regulated sequence, heterology leader, heterology flag sequence or produced fusion rotein.

Nucleic acid molecule of the present invention can be the rna form of mRNA or cRNA for example, perhaps comprises the cDNA that for example obtains by the clone or chemical synthesising technology produces and the dna form of genomic dna or its combination.DNA can be three chains, two strands or strand.Single stranded DNA can be a coding strand, also is known as positive-sense strand, and perhaps it can be a noncoding strand, also refers to antisense strand.

The invention still further relates to the varient of above-described nucleic acid molecule herein, described varient coding has hyper-immuneserum reactive antigen and its segmental fragment, analogue and the derivative of the streptococcus agalactiae aminoacid sequence of the reckoning of listing in the sequence list.The varient of nucleic acid molecule can be the varient of natural generation, the allelic variation body of natural generation for example, and perhaps it can be not know it is the varient of natural generation.Can make the varient that the such non-natural of nucleic acid molecule takes place by induced-mutation technique, comprise those that are applied to nucleic acid molecule, cell or organism.

Among the varient of this point, varient is to be different from above-mentioned nucleic acid molecule, and difference is the nucleic acid of nucleotide substitution, deletion or increase.Substitute, delete or increase and to comprise one or more Nucleotide.Can be at coding or non-coding region or this two area change varients.The change of coding region can produce conservative or non-conserved amino acid substitutes, deletes or increases.The nucleic acid molecule of varient, analogue, derivative or the fragment of preferably encoding or segmental varient, analogue or derivative, described nucleic acid molecule has as the streptococcus agalactiae sequence of listing in the sequence list, wherein with any cooperative programs minority, 5 to 10,1 to 5,1 to 3,2,1 or do not have that amino acid is replaced, deletion or increase.Especially preferred among these is that silence substitutes, increases or deletion, and they do not change the characteristic and the activity of the streptococcus agalactiae polypeptide of listing in the sequence list.Also especially preferred at this point is conservative substituting.

The epi-position that also comprises modification according to peptide of the present invention and fragment, wherein preferably according to for example { Tourdot, S. wait the people, one or two amino acid of given epi-position is modified or substituted to disclosed rule among the 2000}, and the nucleotide sequence of the such epi-position of modifying of coding.

Very clear epi-position according to the present invention has contained the epi-position that is derived from this epi-position by the amino acid exchange that improves, preserves or significantly do not hinder the t cell activation ability of epi-position at least.So but this epi-position has also contained and has not contained the original series that comes from streptococcus agalactiae excite identical or the epi-position of improved t cell responses preferably.These epi-positions refer to " irregular "; They need have to the MHC/HLA molecular mimicry or preferably to bigger avidity, and they need stimulate the ability of the TXi Baoshouti (TCR) that points to original epi-position in similar or preferably stronger mode.

Can obtain irregular epi-position by appropriate design, promptly consider as for example { Rammensee, H. wait the people, the single residue that 1999} describes is to being incorporated into the effect of MHC/HLA, associating may with the systems exchange of the interactional residue of TCR and the sequence that obtains with the T cell tests of the original epi-position of sensing.Such design for those skilled in the art be possible and need not over-drastic test.

Another possibility comprises with the screening of the T cell that points to original epi-position to the peptide storehouse.The site scanning that preferred mode is a synthesis peptide library.Such method is in that for example { Hemmer, people such as B., 1999} have a detailed description and give reference herein.

As the epi-position of this derived amino acid sequence representative or the scheme of selecting fully of irregular epi-position, also can adopt the material of these epi-positions of imitation, for example " peptide dummy " or " replying-anti--peptide " (retro-inverso-peptides).

Improved epi-position design is preparation or the modification that has the material that increases its ability that stimulates the T cell on the other hand.These comprise t helper cell epi-position, lipid or liposome or the preferred modification as describing among the WO01/78767.

The other method that increases epi-position T cytositimulation ability is the preparation that has the immunostimulation material, for example resemble cytokine or chemokines, especially IFN-γ, GM-CSF, TNF-α, flt3 part of interleukin II ,-7 ,-12 ,-18, I and II type Interferon, rabbit (IFN) etc.

Ground is discussed in addition as herein, about nucleic acid molecule test of the present invention, the hybridization probe that nucleic acid molecule of the present invention for example as discussed above can be used as RNA, cDNA and genomic dna to be separating the full-length cDNA and the genomic clone of code book invention polypeptide, and separation and nucleic acid molecule of the present invention have the cDNA and the genomic clone of other gene of very high sequence similarity.Such probe generally comprises at least 15 bases.Preferably, such probe will have at least 20, at least 25 or at least 30 bases, can have at least 50 bases.Particularly preferred probe will have at least 30 bases, will have 50 or base still less, for example 30,35,40,45 or 50 bases.

For example, use known dna sequence dna to separate the coding region of nucleic acid molecule of the present invention with synthetic oligonucleotide probe by screening relevant library.Labeled oligonucleotide with the sequence that is complementary to gene order of the present invention is used to screen cDNA, genomic dna or mRNA library then to measure probe hybridization in which member in library.

As ground further is discussed herein, about the nucleic acid molecule test, nucleic acid molecule of the present invention and polypeptide can be as developing the especially treatment of diseases of human diseases and the reagent and the material of diagnosis.

Nucleic acid molecule of the present invention as oligonucleotide can be used for method described herein, but PCR preferably, and whether the streptococcus agalactiae gene herein differentiated is all or part of is present in the infected tissue of blood for example and/or transcribes therein to measure.Have realized that the infective stage that such sequence also will reach in pathogenic agent and the diagnosis of infection type have purposes.For this and other purpose, can use the array that comprises at least one according to nucleic acid of the present invention as described herein.

Can be used for nucleic acid molecule and contain the organism of these nucleic acid or the detection of sample according to nucleic acid molecule of the present invention.Preferably such detection is in order to diagnose, more preferably be for the existence of streptococcus agalactiae or the diagnosis of abundance disease relevant or that link.

The particularly Mammals that has infected streptococcus agalactiae especially people's eukaryotic cell (herein also " individuality ") is to differentiate, method is the detection according to any nucleic acid molecule of the present invention that detects at dna level by various technology.Can obtain in order to distinguish the preferred candidate nucleic acid molecule of streptococcus agalactiae from other organism.

The invention provides the method for the disease that diagnostic source infects from streptococcus agalactiae, comprise isolating or derive from the increase that individual sample determination has the nucleic acid molecule expression level of the sequence of nucleic acid molecules of listing the sequence list from individuality.Can use nucleic acid molecule quantitative methods well known in the art to measure the expression of nucleic acid molecule, for example PCR, RT-PCR, RNase protection, Northern spot, other hybridizing method and array described herein.

Separate from its native state as isolating being meant " by artificial " of using herein; That is, if its natural generation, it will be changed or remove from its primal environment, or two all possible.For example, the nucleic acid molecule of the natural natural generation that is present in organism alive or peptide are not " isolating " in its native state, but isolating identical nucleic acid molecule or polypeptide are " isolating " from the coexisting substances of its native state, as the term that adopts herein.As separated portions or following separation, for example form fused protein and for propagation among the host or expression for mutagenesis, such nucleic acid molecule can be connected in other the nucleic acid molecule of DNA for example.Isolated nucleic acid molecule separately or be connected in the host cell that other nucleic acid molecule of carrier for example can be introduced into organism cultivation or complete.The host cell of that introduce to cultivate or all biological body, such DNA still will be isolating, as the term that uses herein, because they will not be the forms of their natural generations or not in the environment of their natural generations.Similarly, nucleic acid molecule and polypeptide can occur in the composition, for example medium preparation, nucleic acid molecule or polypeptide are introduced the composition or the solution of for example intracellular solution, chemistry or enzyme reaction, for example, they are not the compositions of natural generation, within the meaning of that term of Cai Yonging, remain isolated nucleic acid molecule or polypeptide there herein.

According to nucleic acid of the present invention can be chemosynthesis.Perhaps, nucleic acid can separate from streptococcus agalactiae by method known to those skilled in the art.

According to a further aspect in the invention, by using antigen discrimination method described herein that the one comprehensive novel hyper-immuneserum reactive antigen of cover and its fragment is provided.In the preferred embodiment of the invention, provide the hyper-immuneserum reactive antigen and the fragment thereof of the aminoacid sequence that comprises any one nucleic acid molecule encoding described herein.In another preferred embodiment of the present invention, provide to comprise to be selected from a novel hyper-immuneserum reactive antigen of cover and a fragment thereof of the aminoacid sequence of the peptide sequence of representing among the 374-433 as Seq ID No 231,307.In another embodiment preferred of the present invention, provide to comprise to be selected from Seq ID No 218,220,222-230,232,235-242,244-248,250-253,256-285,287-302,309-317,320-343,345-362,364,366-373,434, a novel hyper-immuneserum reactive antigen of cover and a fragment thereof of the aminoacid sequence of the peptide sequence of representing among 449-462 and the 475-486.In another preferred embodiment of the present invention, provide a novel hyper-immuneserum reactive antigen of cover and a fragment thereof that comprises the aminoacid sequence that is selected from the peptide sequence of expression among the Seq ID No 249,303,308,318,344.

Comprise any polypeptide of listing in the sequence list as hyper-immuneserum reactive antigen provided by the invention and fragment thereof, and following polypeptide: have with sequence list in polypeptide at least 70% homogeny listed, preferably with sequence list in polypeptide at least 80% or 85% homogeny listed, more preferably with sequence list in polypeptide at least 90% similarity (more preferably at least 90% homogeny) listed and still more preferably with sequence list in the polypeptide at least 95% listed, 96%, 97%, 98%, 99% or 99.5% similarity (still more preferably at least 95%, 96%, 97%, 98%, 99% or 99.5% homogeny), the part that also comprises such polypeptide, described polypeptide generally has and contains 4 amino acid, more preferably at least 8, still more preferably at least 30, still at least 50 amino acid more preferably, for example 4,8,10,20,30,35,40,45 or 50 amino acid whose such polypeptide portions.

The invention still further relates to these hyper-immuneserum reactive antigen and segmental fragment, analogue and derivative.Term " fragment ", " derivative " and " analogue " are meant to have kept basically and such hyper-immuneserum reactive antigen and identical or similar biological function of fragment or active polypeptide when referring to its aminoacid sequence when listing in antigen in the sequence list.

Hyper-immuneserum reactive antigen and segmental fragment thereof, derivative or analogue can be 1) wherein one or more amino-acid residues quilts are conservative or non-conservative amino acid residues (preferably Bao Shou amino-acid residue) alternate is a kind of, and the alternate amino-acid residue can be or can not be one that genetic code is encoded like this, perhaps 2) wherein one or more amino-acid residues comprise a kind of of substituted radical, perhaps 3) wherein sophisticated hyper-immuneserum reactive antigen and fragment thereof and for example increase the hyper-immuneserum reactive antigen and the compound of fragment transformation period (for example, polyoxyethylene glycol) it is a kind of that another kind of compound merges, perhaps 4) wherein extra amino acid is blended in sophisticated hyper-immuneserum reactive antigen and segmental a kind of, for example guide or secretion sequence or be used for the sequence or the preceding protein sequence of ripe hyper-immuneserum reactive antigen and segmental purifying thereof.Such fragment, derivative and analogue are thought those skilled in the art herein in the scope of religious doctrine.

The present invention also relates to the antigen of different streptococcus agalactiae isolates.Can separate such homologue based on nucleic acid disclosed herein at an easy rate with aminoacid sequence.9 distinct serotypes are arranged so far, and somatotype is based on the serotype specificity antiserum(antisera).Can correspondingly measure any antigenic existence for each serotype.In addition, { variability of specific antigen is possible to mensuration in the various serotypes that 2001} describes for Hoe, people such as N. as purulence suis (S.pyogenes) sic gene.Various serotypes are different to the age groups that acts on that different GBS infect with the geographic area.The protection antigen of expectation most worthy among various clinical strains be of guarding very important aspect.

Hyper-immuneserum reactive antigen and varient, analogue, derivative and the fragment in this point sequence tabulation, listed, and segmental varient, analogue and derivative are in the especially preferred embodiment of the present invention.Extraly, the fusion polypeptide that comprises such hyper-immuneserum reactive antigen and varient, analogue, derivative and fragment and segmental varient, analogue and derivative is also contained among the present invention.The use standard technique can be made such fusion polypeptide and protein and their nucleic acid molecule of encoding at an easy rate, comprises the standard recombinant technology of producing and expressing the reorganization polynucleic acid of encoding fusion protein matter.

Those different with reference are among preferred varient because conserved amino acid substitutes.Like this alternative be in the polypeptide given amino acid by those of the amino acid replacement of another same characteristic.Generally being considered as conservative substituting is fatty amino acid Ala, Val, mutual replacement among Leu and the Ile; The exchange of hydroxyl residue Ser and Thr, the exchange of acidic residues Asp and Glu, substituting between amide residues Asn and the Gln, the replacement between the exchange of alkaline residue Lys and Arg and aromatic base residue Phe and the Tyr.

Further especially preferred in this is varient, analogue, derivative and the fragment with aminoacid sequence of any polypeptide of listing in the sequence list, and segmental varient, analogue, derivative, wherein several, minority, 5 to 10,1 to 5,1 to 3,2,1 or do not have that amino-acid residue is replaced with any cooperative programs, deletion or increase.Especially preferred among these is that characteristic and the active silence that does not change polypeptide of the present invention substitutes, increases and deletion.Also especially preferred in this is conservative substituting.Topnotch preferably has the polypeptide of listing in the sequence list that does not have the alternate aminoacid sequence.

Preferably provide hyper-immuneserum reactive antigen of the present invention and fragment thereof with isolating form, preferably purifying is a homogeneity.

The fragment that comprises the varient of the polypeptide of listing in the fragment of the polypeptide with aminoacid sequence of listing in the sequence list and the sequence list and derivative is also in the preferred embodiment of the invention.

In this, fragment is to have with above-mentioned hyper-immuneserum reactive antigen and segmental part thereof but the polypeptide of the identical aminoacid sequence of not all aminoacid sequence and varient thereof or derivative, analogue, fragment.Such fragment can be " free ", promptly be not other amino acid or polypeptide part or with its fusion, perhaps they are also contained in the bigger polypeptide in a part that their form or zone.This aspect of the present invention also preferably is characterised in that the structure of polypeptide of the present invention or the fragment of functional attributes, promptly comprise the alpha-helix of polypeptide of the present invention and alpha-helix form zone, beta sheet and beta sheet form the zone, turn back and turn back form the zone, curl and curl into zone, hydrophilic region, hydrophobic region, α both sexes zone, β both sexes zone, can bent (flexible) zone, the surface forms zone, substrate calmodulin binding domain CaM and the fragment of high antigen indicating area and so segmental associating.Preferred zone be mediation hyper-immuneserum reactive antigen of the present invention and segmental active those.Topnotch preferably has hyper-immuneserum reactive antigen of the present invention and segmental chemistry, biology or other active fragment in this, include similar activity improve active or have the non-expectation of reduction active those.Especially preferred is the fragment that comprises enzyme acceptor or structural domain, and described enzyme acceptor or structural domain are given the streptococcus agalactiae survival or caused the necessary function of ability of human diseases.Further preferred polypeptide fragment is to comprise or contain those of antigen in people's especially the animal or immunogenicity determinant.

Antigen fragment is defined as the antigenic fragment of discriminating, and it is antigenic or can makes antigenic when being provided as haptens for itself.So, the present invention has realized antigen or the antigen fragment that one of demonstration or (for longer fragment) have only a few amino acids to exchange, suppose seriously to destroy so segmental antigenic capacity that the amino acid exchange is arranged, promptly be suitable for causing the suitable immune response of the individuality of accepting this antigen immune inoculation and use from the individual antibody preparations of individual serum and differentiate in when exchange.

All these fragments individually and each formed the aspect that the present invention preferably selects independently.

There is the full sequence of 1 amino acid eclipsed peptide analysis proteantigen can differentiate all linear hyper-immuneserum fragment reactions of specific antigen by at least 10 amino acid of a cover length.Subsequently, can differentiate non-linear epi-position by the full length protein of use expressing or its structural domain polypeptide with hyper-immuneserum analysing protein antigen.Hypothetical protein matter particular structure territory enough forms the 3D structure that does not rely on natural protein, and each reorganization or the synthetic structural domain polypeptide that produces will allow the discriminating in the conformation antigenic structure territory in each structural domain of Multidomain protein with the analysis of hyper-immuneserum.Have those antigens of linearity and conformational epitope for structural domain, use the existence that can be used for confirming the conformational epitope corresponding to the competition experiments of the peptide of linear epitope.

To understand the present invention and especially also relate to the above-mentioned segmental nucleic acid molecule of coding, the nucleic acid molecule of the nucleic acid molecule of hybridization encode fragment, particularly those of hybridize under stringent condition, and the nucleic acid molecule that is used for for example PCR primer of the segmental nucleic acid molecule of amplification coding.On these aspects, preferred nucleic acid molecule be as discussed above corresponding to preferred segmental those.

The present invention also relates to comprise one or more nucleic acid molecule of the present invention carrier, carry out engineered host cell and hyper-immuneserum reactive antigen and segmental generation thereof by recombinant technology with carrier of the present invention.

A large amount of expression vectors can be used for expressing according to hyper-immuneserum reactive antigen of the present invention and fragment thereof.Generally, be suitable for keeping, breeding or express nucleic acid can be used in this respect expression with any carrier of express polypeptide in the host.Consistent with this aspect of the present invention, carrier can for example be plasmid vector, list or double stranded phage carrier, list or double-stranded RNA or dna viral vector.Initial plasmid disclosed herein or commercially available obtainable, disclose obtainablely, perhaps use can be from obtainable plasmid construction for the routine by well-known disclosed program.In some aspects, among the carrier preferably for those of nucleic acid molecule of the present invention and hyper-immuneserum reactive antigen or its segmental expression.Nucleic acid construct physical efficiency in the host cell is used to produce the gene product of recombination sequence coding in a conventional manner.Perhaps, hyper-immuneserum reactive antigen of the present invention and fragment thereof can produce by traditional peptide synthesizer is synthetic.Sophisticated albumen mass-energy is expressed in mammalian cell, yeast, bacterium or other cell under suitable promotor control.Use derives from the RNA of DNA construct of the present invention, also can adopt acellular translation system to produce such protein.

Can carry out genetically engineered to host cell to integrate nucleic acid molecule and to express nucleic acid molecule of the present invention.The representative example of suitable host comprises for example suis, staphylococcus, intestinal bacteria, streptomycete and subtilis bacterial cell; The fungal cell of yeast cell and Aspergillus cell for example; The insect cell of fruit bat S2 and noctuid Sf9 cell for example; CHO for example, COS, Hela, C127,3T3, BHK, 293 and the zooblast of Bowes melanoma cells; And vegetable cell.

The present invention also provides generation streptococcus agalactiae hyper-immuneserum reactive antigen and segmental method thereof, comprises hyper-immuneserum reactive antigen or its fragment from host cell expression nucleic acid molecule encoding provided by the invention.The present invention also provides and has produced the method for expressing streptococcus agalactiae hyper-immuneserum reactive antigen or its segmental cell, comprise with support according to the present invention transform or the transfection proper host cell make transform or the cells transfected expression vector in the polypeptide of the nucleic acid encoding that contains.

Can be with the modified forms express polypeptide of for example fused protein, and polypeptide can comprise not only that secretion signal also has extra heterology functional area.Thereby for example, especially the additional amino acid zone of charge residue can be added to the N of polypeptide or C-terminal to improve stability in host cell and persistence in purge process or in operation subsequently and the storage process.The zone also can be added to polypeptide to promote purifying.Such zone can be removed before the last preparation of polypeptide.Peptide group be added to polypeptide with cause secretion or drain, with improve stability or promote purifying among other be this area that be familiar with technology routine.It is the useful heterology zone from immunoglobulin (Ig) that preferred fusion protein matter comprises for dissolving or purified polypeptide.For example, EP-A-O 464 533 (Canadian copy 2045869) discloses the fused protein of the constant region different piece that comprises immunoglobulin molecules and another protein or its part.In drug discovery, for example, for the target protein matter of the high throughput screening assay of differentiating antagonist with the antibody Fc meromixis.See for example { Bennett, people such as D., 1995} and { Johanson, people such as K., 1995}.

Can from the reconstitution cell culture, regain and purifying streptococcus agalactiae hyper-immuneserum reactive antigen or its fragment by well-known method, comprise ammonium sulfate or ethanol sedimentation, acid extraction, negatively charged ion or cation-exchange chromatography, phosphocellulose chromatography, hydrophobic interaction chromatograph, hydroxylapatite chromatography and lectin chromatogram.

Can produce according to hyper-immuneserum reactive antigen of the present invention and fragment thereof by chemosynthesis and by the biotechnology means.The latter has comprised with the carrier transfection or the transformed host cell that contain with good grounds nucleic acid of the present invention, and cultivates transfection or transformed host cells under condition well known by persons skilled in the art.For purifying or the production method of separating the polypeptide that will produce also can comprise purification step.Carrier is a support according to the present invention in the embodiment preferred.

Can be used for containing the detection of one or more organisms in the sample of these organisms or its polypeptides derived according to hyper-immuneserum reactive antigen of the present invention and fragment thereof.Preferred such detection is in order to diagnose, and more preferably for the diagnosis of disease, most preferably for the diagnosis of or the disease that link relevant with the existence of gram positive bacterium or abundance, especially is selected from the bacterium of suis, glucose coccus and galactococcus.More preferably, microorganism is selected from streptococcus pneumoniae (Streptococcus pneumoniae), streptococcus pyogenes (Streptococcus pyogenes) and streptococcus mutans (Streptococcus mutans), and especially microorganism is a streptococcus pyogenes.

The present invention also relates to diagnostic test, for example be used for detecting the quantitative and diagnostic test of cell and tissue hyper-immuneserum reactive antigen of the present invention and segmental level thereof, comprise the mensuration of normal and abnormal level.So, for example,, in order to detect the existence that the diagnostic test of comparing the overexpression of polypeptide with the healthy tissues sample can be used to detect infection, and differentiate the infection biological body according to the present invention.The experimental technique that can be used for measuring the sample polypeptide level that is derived from the host is well-known for those skilled in the art.Such test method comprises that radioimmunoassay, competition are in conjunction with test, Western spot analysis and ELISA test.Among these, ELISA usually is preferred.The ELISA test comprises antibody, the especially monoclonal antibody that preparation is specific to polypeptide at first.In addition, usually prepared the report antibody that is incorporated into monoclonal antibody.Report antibody is attached to detection reagent, for example radioactivity, fluorescence or enzyme reagent, for example horseradish peroxidase.

Can be used for the purpose of array or coupled according to hyper-immuneserum reactive antigen of the present invention and fragment thereof.More special, at least one can be fixed on the upholder according to hyper-immuneserum reactive antigen of the present invention and fragment thereof.Described upholder generally comprises various hyper-immuneserum reactive antigen and fragment thereof, can use a kind of thus or some produce diversity according to hyper-immuneserum reactive antigen of the present invention and fragment thereof and/or different hyper-immuneserum reactive antigen and fragments thereof.The such array and the features characteristic of any array generally be unique polypeptide be fixed in described upholder or its surface go up unique or predefined zone or position on.Because this, any activity on array unique location or the zone is relevant with specific polypeptide.Different hyper-immuneserum reactive antigen and fragment thereof are fixed in the quantity of upholder can be so few to several thousand in different hyper-immuneserum reactive antigen and the segmental scope thereof from 10.Every cm in the preferred embodiment ²Hyper-immuneserum reactive antigen and segmental density thereof are from every cm ²10 peptide/polypeptide arrive every cm so less ²At least 400 different peptide/polypeptide, special more every cm ²At least 1000 different hyper-immuneserum reactive antigen and fragment thereof.

The production of this array it is known to those skilled in the art that, for example, at United States Patent (USP) 5,744, description arranged in 309.Array preferably comprises smooth, porous or the non-porous solid support with first surface at least.So go out disclosed hyper-immuneserum reactive antigen and fragment thereof and be fixed in described surface.Preferred support material is glass or Mierocrystalline cellulose among other.For any diagnostic use described herein uses matrix also in the present invention.Except hyper-immuneserum reactive antigen according to the present invention and fragment thereof, for the generation of array as described above also can be used according to nucleic acid of the present invention.This also is applied to the array that antibody is made, preferably among other as monoclonal antibody described herein.

The present invention relates to point to any on the other hand according to hyper-immuneserum reactive antigen of the present invention and fragment, its derivative or segmental antibody.For example the present invention includes mono-clonal and polyclonal antibody, chimeric, strand and humanized antibody, and the product of Fab fragment or Fab expression library.Antibody can be chimeric also within the present invention, and promptly their different piece is derived from different genera or sequence is at least separately taken from different kinds.

By with the hyper-immuneserum reactive antigen and fragment is injected directly into animal or by giving preferred inhuman animal hyper-immuneserum reactive antigen and fragment thereof, anti-the hyper-immuneserum reactive antigen and the segmental antibody thereof corresponding to sequence of the present invention that can obtain to produce.The antibody that so obtains then will be in conjunction with hyper-immuneserum reactive antigen and fragment itself thereof.In this mode, even only encode hyper-immuneserum reactive antigen and a segmental fragments sequence thereof can be used for producing in conjunction with whole natural hyper-immuneserum reactive antigen and segmental antibody thereof.So then antibody capable is used for separating hyper-immuneserum reactive antigen and fragment thereof from expressing those hyper-immuneserum reactive antigen and segmental tissue thereof.

For MONOCLONAL ANTIBODIES SPECIFIC FOR, can use any technology that the antibody that the continuous cell line culture produces is provided known in the art (as original Kohler, people such as G. describe ground among the 1975}).

It is anti-according to immunogenic hyper-immuneserum reactive antigen of the present invention and segmental single-chain antibody thereof to make the technology of describing for the generation of single-chain antibody be adapted to produce.Transgenic mice or for example other mammiferous other organism can be used for expressing anti-according to immunogenicity hyper-immuneserum reactive antigen of the present invention and segmental humanized antibody thereof.

Perhaps, display technique of bacteriophage or ribosomal display can be used for having the human lymphocytic pcr amplification v-gene library of target antigen separately or selecting from inmature library from screening hyper-immuneserum reactive antigen and fragment thereof are had in conjunction with active antibody gene { McCafferty, J. wait people, 1990}; { Marks, people such as J., 1992}.{ Clackson, people such as T., 1991} improve the avidity of these antibody also to reorganize (chain shuffling) by chain.

If there are two antigen binding domainss, each structural domain can point to different epi-position-be called " dual specific " antibody.

Above-described antibody can be applied to separate or differentiate express hyper-immuneserum reactive antigen and segmental clone thereof or for the separation of affinity chromatography and/or purifying by antibody is attached to solid support and purifying hyper-immuneserum reactive antigen of the present invention and fragment thereof.

Thereby anti-hyper-immuneserum reactive antigen of the present invention and segmental antibody thereof especially can be applied to suppress and/or treatment is infected, and infectation of bacteria particularly is particularly from the infection of streptococcus agalactiae.

Hyper-immuneserum reactive antigen and fragment thereof comprise the derivative that antigenicity, epi-position or immunogenicity are equal to, and it has formed particular aspects of the present invention.The hyper-immuneserum reactive antigen and fragment or its equivalent that comprise some antibodies specific identification herein as the term " antigenicity equivalent derivatives " that uses, described antibody disturbs the interaction between pathogenic agent and the mammalian hosts when at protein according to the present invention or hyper-immuneserum reactive antigen and fragment cultivation thereof.Comprise peptide or its equivalent as the term " immunogenicity equivalent derivatives " that uses herein, when it is used for appropriate formulation with when spinal animals is cultivated antibody, antibody works the interactional effect between pathogenic agent and the mammalian hosts of disturbing.

Hyper-immuneserum reactive antigen and fragment thereof, for example antigenicity or immunogenicity equivalent derivatives or its fusion rotein can be as antigen with immune mouse or other animals of rat or chicken for example.Fused protein provides stability can for hyper-immuneserum reactive antigen and fragment thereof.Antigen can be for example by in conjunction with the immunogenic carrier protein that is connected in bovine serum albumin (BSA) for example or keyhole limpet hemocyanin (KLH).Perhaps, comprising hyper-immuneserum reactive antigen and the fragment thereof that the antigen peptide of protein or hyper-immuneserum reactive antigen and a plurality of copies of fragment thereof or antigenicity, immunogenicity be equal to can have enough antigenicities to avoid the use of carrier to improve immunogenicity.

Preferably, the modified antibodies or derivatives thereof makes it at individuality still less immunogenicity be arranged.For example, if individuality is the people, antibody most preferably can be " humanized ", wherein the complementarity determining region of hybridoma deutero-antibody is inoculated into human monoclonal antibodies, for example as { Jones, people such as P., 1986} or { Tempest, people such as P., description ground among the 1991}.

Suitable delivering method will be preferably adopted in the use of polynucleotide of the present invention in genetic immunization, and for example plasmid DNA is injected directly into muscle, with sending of specific protein carrier compound DNA, DNA co-precipitation with calcium phosphate, DNA is packaged in the various forms of liposomes, particle bombardment { Tang, people such as D., 1992}, { Eisenbraun, people such as M., 1993} and the interior { Seeger of infection of body that uses clone's reverse road virus vector, C. wait people, 1984}.

The present invention relates on the other hand be incorporated into according to any hyper-immuneserum reactive antigen of the present invention and segmental peptide thereof, and the production method of such peptide, described thus method feature is according to hyper-immuneserum reactive antigen of the present invention and segmental use thereof, and basic steps it is known to those skilled in the art that.

Can produce such peptide, for example phage display or ribosomal display by using method according to art technology.Under the situation of phage display, produced the library of the peptide of phage form basically, this library contacts with target molecule, is according to hyper-immuneserum reactive antigen of the present invention and fragment thereof under this situation.From reaction separately, remove those subsequently and be incorporated into the peptide of target molecule, preferably with the mixture of target molecule.The bonded characteristic is well known by persons skilled in the art in the experiment setting that certain degree depends on for example specific implementation of salt concn or the like at least.Those peptides of target molecule that are incorporated into higher avidity or bigger strength are from non-binding member's after separating in library, and alternatively also after target molecule is removed from the mixture of target molecule and peptide, subsequently can signature analysis peptide separately.Before the signature analysis, carry out amplification step alternatively, for example by breeding the phage of encoded peptide.Signature analysis preferably comprises the order-checking of target binding peptide.Basically, peptide does not limit their length, yet preferably preferably obtains having the peptide from about 8 to 20 amino acid whose length in method separately.The size in library can be about 10 ²To 10 ¹⁸, preferably 10 ⁸To 10 ¹⁵Individual different peptide, however be not limited to these.

The targeted integration hyper-immuneserum reactive antigen of particular form and fragment thereof are so-called " anti-calines ", and they have description in German patent application DE 197 42 706 among other.

The present invention relates on the other hand with any according to hyper-immuneserum reactive antigen of the present invention and the interactional functional nucleic acid of fragment thereof, and the production method of such functional nucleic acid, described thus method feature is according to hyper-immuneserum reactive antigen of the present invention and segmental purposes thereof, and basic steps it is known to those skilled in the art that.Functional nucleic acid is preferably fit and specular surface body.

Fit is strand or double-stranded and specificity and the interactional D-nucleic acid of target molecule.Fit production or select and for example in the European patent EP 0 533 838 description is being arranged.Basically the step below having carried out.At first, provide the mixture of nucleic acid, promptly potential is fit, and each nucleic acid generally comprises some preferably at least 8 at random fragments of Nucleotide subsequently thus.This mixture contacts with target molecule subsequently, thus for example based on combining with target molecule to the avidity of target spot increase or to the bigger strength nucleic acid of target spot with candidate's mixture.Separation and combination nucleic acid from remaining mixture subsequently.Alternatively, the nucleic acid that uses for example polymerase chain reaction amplification to obtain like this.These steps can repeat for several times, as long as final mixture has the ratio that the specificity of increase is incorporated into the nucleic acid of target spot, therefrom select final bind nucleic acid then alternatively.These specificity bonded nucleic acid refer to fit.Clearly, can gather the sample of individual nucleic acid mixture in order to use its sequence of measured by standard techniques in any stage of fit production or discrimination method.For example can stablize fit within the present invention to the chemical group that produces fit technical field definition known to the skilled by introducing.Such modification for example may reside in the introducing of amino group in nucleotide sugar group 2 ' position.Fit at present as therapeutical agent.Yet, select like this or produce fit can be used for that target spot is confirmed and/or as guide's material of the medicament exploitation that is preferably based on the small molecules medicament also within the present invention.In fact this finish by competition experiments, thus drug candidate suppress target molecule and fit between specificity interact, thus fit when target spot and fit mixture replace, can suppose separately drug candidate allow target spot and fit between interactional specificity suppress, if interact is specific, and described drug candidate will be suitable for blocking target spot at least in principle and thereby reduce its bioavailability or activity in comprising the system separately of such target spot.The small molecules that obtains like this can carry out further derivatize and modify to optimize its physics, chemistry, biology and/or medical features, for example toxicity, specificity, biological degradability and bioavailability.

Specular surface body and their generation or manufacturing are based on a simple principle.Being manufactured in the International Patent Application WO 98/08856 of specular surface body has description.Specular surface body is a L-nucleic acid, and this is meant that they are by L-Nucleotide rather than resemble fit D-Nucleotide and form.Specular surface body be characterised in that they biosystem have very high stability and with the fit target molecule that points to their this fact that interacts of comparing specifically.Produce in the method for specular surface body, produced the allos group of D-nucleic acid, this group contacts with the optical antipode of target molecule, for example contacts with the D-enantiomorph of the L-enantiomorph of hyper-immuneserum reactive antigen and segmental natural generation thereof according to the present invention under this situation.Subsequently, separate not and interactional those D-nucleic acid of the optical antipode of target molecule.But separate, differentiate alternatively and/or interactional those D-nucleic acid of optical antipode of order-checking and target molecule, synthesize corresponding L-nucleic acid based on the nucleic acid sequence information that obtains from D-nucleic acid subsequently.Will be specifically interact with regard to these identical with regard to the sequence of the interactional above-mentioned D-nucleic acid of the optical antipode of target molecule L-nucleic acid with the target molecule rather than its optical antipode of natural generation.Similar to the method for fit generation, repeat various steps several times and thereby interactional those nucleic acid of optical antipode of concentrate specificity and target molecule be possible.

On the other hand, the present invention relates to according to the interactional functional nucleic acid of any nucleic acid molecule of the present invention, and the manufacture method of such functional nucleic acid, described thus method feature is that the purposes and the basic steps of sequence are known for those skilled in the art separately with them according to nucleic acid molecule of the present invention.Functional nucleic acid is ribozyme, antisense oligonucleotide and siRNA preferably.

Ribozyme is the nucleic acid of catalytic activity, preferably is made up of the RNA that comprises two groups basically.First group has shown catalytic activity and second group is responsible for interacting with the specificity of target nucleic acid, is that coding is according to hyper-immuneserum reactive antigen of the present invention and segmental nucleic acid thereof under this situation.When target nucleic acid and ribozyme second group interact, general by the extension of complementary base hybridization and Watson-Crick base pairing basically on two hybridization chains, the catalytic activity group can become activated, just be meant it or intramolecularly or intermolecular catalytic nucleic acid molecule, if the catalytic activity of ribozyme is a phosphodiesterase activity.Subsequently, can there be target nucleic acid further to degrade, the proteinic degraded that has caused target nucleic acid at last and be derived from described target nucleic acid.Ribozyme, their use and principle of design are known for those skilled in the art, and for example { 2001} is with { Lewin, people such as A. have description among the 2001} for Doherty, people such as E..

Be used for the manufacturing of medicament and as the activity of the antisense oligonucleotide of diagnostic reagent and design respectively based on the model identical of effect.Basically, antisense oligonucleotide is preferably hybridized with mRNA based on base complementrity and target RNA, thereby activates RNase H.RNase H is activated by phosphodiester and thiophosphatephosphorothioate link coupled DNA.Yet except thiophosphatephosphorothioate link coupled DNA, phosphodiester link coupled DNA is soon by the intracellular nucleic enzyme liberating.The DNA derivative takes place and do not suppress RNase H when hybridizing with RNA in the non-natural of these resistances.In other words, antisense polynucleotide is effective as just DNA RNA hybridization complex.U.S. Pat 5,849,902 and the US 5,989,912 of the example of this antisense oligonucleotide among other.In other words, nucleotide sequence based on target molecule, target molecule is according to hyper-immuneserum reactive antigen of the present invention and segmental nucleic acid molecule thereof under this situation, can be based on the principle of base complementrity, in principle can be from the target protein of the nucleotide sequence separately of its supposition or by understanding the especially so suitable antisense oligonucleotide of nucleotide sequence design of mRNA.

Particularly preferably be the have phosphorothioate dna antisense oligonucleotide of extension of weak point of (3 to 9 bases).Minimum 3 DNA bases are essential for the activation of bacterium RNase H, and minimum 5 bases are that Mammals RNase H activation is essential.The central zone that forms RNase H substrate is arranged in these chimeric oligonucleotides, and it joins with hybridization " arm " side of being made up of the Nucleotide of the modification that does not form RNase H substrate.Can for example pass through the hybridization arm of 2 '-oxygen-methyl or 2 '-fluorine modified chimeric oligonucleotide.The alternate method has used methylphosphonate or the phosphoramidate in the described arm to connect.The useful further embodiment of antisense oligonucleotide is a P-methoxyl group oligonucleotide in the practice of the present invention, few thymus nucleic acid of part P-methoxyl group or P-methoxyl group oligonucleotide.

Especially relevant and those antisense oligonucleotides described more especially of two United States Patent (USP)s as mentioned above usefully of the present invention.These oligonucleotide do not contain 5 of natural generation ' → 3 '-Nucleotide that connects.More suitably oligonucleotide has the Nucleotide of two types: activate 2 '-deoxidation thiophosphatephosphorothioate of RNase H and do not activate the Nucleotide of the 2 ' modification of RNase H.Connection between the Nucleotide of 2 '-modification is phosphodiester, thiophosphatephosphorothioate or P-oxyethyl group phosphodiester.Finish the activation of RNase H by successive RNase H active region, described active region contains 2 ' deoxidation thiophosphatephosphorothioate Nucleotide between 3 and 5 to activate 2 ' deoxidation thiophosphatephosphorothioate Nucleotide between bacterium RNase H and 5 and 10 to activate particularly Mammals RNase H of eucaryon.By making 5 ' and 3 ' terminal bases height ribozyme resistance and finishing for the protection of degrading by replacing 3 ' terminal blocking group alternatively.

More especially, the few nucleic acid of antisense comprises 5 ' terminal and 3 ' end; From the position 11 to 595 ' → 3 '-Nucleotide that connects is independently selected from 2 ' phosphodiester Nucleotide of modifying and 2 ' the P-alkoxyl group phosphotriester Nucleotide of modifying; Wherein 5 ' terminal nucleotide is attached to the RNase H active region between the thymus nucleic acid that three and ten successive thiophosphatephosphorothioates connect, and wherein said Nucleotide 3 ' end is selected from continuous extension, vitamin H group and the P-alkoxyl group phosphotriester Nucleotide of the Yeast Nucleic Acid that the thymus nucleic acid of counter-rotating, one to three thiophosphatephosphorothioate 2 ' modify.

Can use antisense oligonucleotide, wherein be not 5 ' terminal nucleotide but the 3 ' terminal nucleotide that describes in detail above is attached to RNase H active region yet.5 ' end also is selected from specific group, rather than 3 ' end of described oligonucleotide.

According to nucleic acid of the present invention and hyper-immuneserum reactive antigen and fragment thereof can as or be used for the especially manufacturing of the pharmaceutical composition of vaccine.That preferably cause, the relevant or prevention and treatment of diseases that links of such pharmaceutical composition of vaccine preferably for streptococcus agalactiae.Another aspect of the present invention relates to the immunoreactive method of inducing especially mammiferous individuality in this scope; comprise with hyper-immuneserum reactive antigen of the present invention and fragment or its fragment or varient inoculation individuality; enough producing antibody avoids infecting particularly streptococcal infection and the most special streptococcic infection to protect described individuality.

Still another aspect of the present invention; relate to the method for inducing the individual immunity reaction; comprise by gene therapy or other; nucleic acid delivery functional coding hyper-immuneserum reactive antigen and fragment thereof or its fragment or varient are with expression in vivo hyper-immuneserum reactive antigen and fragment or its fragment or varient; purpose is in order to induce the immune response that produces antibody or cell-mediated t cell responses to avoid disease to protect described individuality; no matter whether that disease determines in individual that the T cell can be T cell or the cytotoxic T cell that produces cytokine.A method that gives gene is to enter in the cell of expectation as coating on the particle or other gene by acceleration.

Another aspect of the present invention relates to immune composition, when being introduced into to induce in the host in the immunoreactive host time, it can induce the immune response in such host, and wherein said composition comprises coding and expresses hyper-immuneserum reactive antigen of the present invention and segmental antigenic recombinant DNA thereof.Can therapeutic or prophylactically use immune response, can adopt the form of antibody mediated immunity or cellular immunization, for example be derived from the cellular immunization of CTL or CD4+T cell.

But hyper-immuneserum reactive antigen of the present invention and fragment thereof or its fragment can merge with the auxilliary protein (co-protein) that itself may not produce antibody can stablize first protein and produce the fused protein will have immunity and protection feature.The recombinant protein of this fusion preferably further contains the auxilliary protein of antigenicity, for example glutathione s-transferase (GST) or beta-galactosidase enzymes, and solubilising protein also promotes it to produce and the relative big auxilliary protein of purifying.In addition, auxilliary protein plays adjuvant on the meaning that provides immunity system comprehensively to stimulate.Auxilliary protein can be attached to first proteinic aminoterminal or carboxyl terminal.

The present invention also provides in the streptococcus agalactiae animal models infected and use described nucleic acid molecule or its specific segmental method in such genetic immunization experiment.Such fragment is for differentiating that the protein epitope that can cause preventative or therapeutic immune response is particularly useful.This method can allow have the monoclonal antibody of special value to handle with the prevention reagent or the treatment of streptococcus agalactiae infection the Mammals of the exploitation especially mankind from the indispensable organ preparation of successfully resisting or removing infected animals subsequently.

Hyper-immuneserum reactive antigen and fragment thereof can be used as the specific antibody of the antigen of host immune inoculation with the protection of generation antibacterium invasion, for example by blocking the adhesion of cell to damaged tissue.The example of tissue injury comprises the (respiratory system especially of virus infection for example, influenza virus for example), the skin that causes of machinery, chemistry or thermal damage or the implantation by indwelling equipment or the wound of reticular tissue and mucosal tissue, the perhaps wound of the mucous membrane of oral cavity, mammary gland, urethra or vagina for example.

The present invention also comprises the vaccine preparation that comprises immunogenicity recombinant protein and suitable carrier.Because protein can be degraded in stomach, preferably it gives by parenteral route, comprises in for example subcutaneous, intramuscular, vein, intracutaneous, the nose or giving through skin.Be suitable for the preparation that parenteral route gives and comprise water-based and non-aqueous aseptic injectable solution, it can contain and gives body fluid isoosmotic antioxidant, buffer reagent, bacterial inhibitor and the solute of preparation with individual preferably blood; Water-based and nonaqueous sterile suspensions, it can comprise suspension agent or thickening material.Preparation can be presented in the container of unitary dose or a plurality of dosage, and for example Mi Feng ampoule and medicine bottle can be stored under the lyophilize condition, adds sterile liquid carrier immediately before only needing to use.Vaccine preparation also can comprise the immunogenic adjuvant system of enhancing preparation, for example oil-in-water system and other system known in the art.Dosage will depend on the activity specific of vaccine and can measure by routine test at an easy rate.

According on the other hand, the present invention relates to comprise as the present invention for the hyper-immuneserum reactive antigen and the segmental pharmaceutical composition thereof of streptococcus agalactiae providing.Such pharmaceutical composition can comprise preferably a hyper-immuneserum reactive antigen and the fragment thereof of two or more at least anti-streptococcus agalactiaes.Optionally, such streptococcus agalactiae hyper-immuneserum reactive antigen and fragment thereof also can be with anti-other pathogenic agent in combined medicinal composition antigen combined.Preferably, described pharmaceutical composition is in order to prevent or to treat the vaccine of the infection that streptococcus agalactiae and/or other pathogenic agent cause, having comprised the antigen of anti-described pathogenic agent in the vaccine.

According on the other hand, the present invention relates to comprise that coding differentiates above for the hyper-immuneserum reactive antigen of streptococcus agalactiae or the pharmaceutical composition of its segmental nucleic acid molecule.Such pharmaceutical composition can comprise hyper-immuneserum reactive antigen or its segmental nucleic acid molecule of the anti-streptococcus agalactiae of one or more codings.Alternatively, coding hyper-immuneserum reactive antigen or its segmental such streptococcus agalactiae nucleic acid molecule also can be united with the antigenic nucleic acid molecule of anti-other pathogenic agent of coding in combined medicinal composition.Preferably, described pharmaceutical composition is in order to prevent or to treat the vaccine of the infection that streptococcus agalactiae and/or other pathogenic agent cause, having comprised the antigen of anti-above-mentioned pathogenic agent in the vaccine.

Pharmaceutical composition can contain any suitable auxiliary substance, for example buffer substance, stablizer or other activeconstituents, the especially known composition relevant with pharmaceutical composition and/or production of vaccine.

Preferred carrier and/or vehicle according to hyper-immuneserum reactive antigen of the present invention, fragment or its coding nucleic acid molecule are in order further to stimulate the immunoreactive immune-stimulating compound to given hyper-immuneserum reactive antigen, its fragment or its coding nucleic acid molecule.Preferably be selected from the range of polycationic substances of polycation peptide especially, preferably immunostimulatory nucleic acid molecule, alum, Freund's complete adjuvant, Neurologically-active compounds or its combination of Freund's incomplete adjuvant, especially human growth hormone of immunostimulation deoxynucleotide according to the immune-stimulating compound in the pharmaceutical preparations of the present invention.

Especially the pharmaceutical composition of vaccine except that hyper-immuneserum reactive antigen according to the present invention, its fragment and/or its coding nucleic acid molecule, also include biology or pharmaceutical activity other compound also within the scope of the invention.Preferably, vaccine composition comprises at least one polycation peptide.Polycationic compounds used according to the invention can be any polycationic compounds that has shown according to the characteristic action of WO 97/30721.Preferred polycationic compounds is selected from basic polypeptide, organic polycation, alkaline polyamino acid or their mixture.These polyamino acid should have the chain length (WO 97/30721) of at least 4 amino-acid residues.Especially preferred is to resemble polylysine, poly arginine and especially containing more than 20% especially more than the material of the polypeptide of 50% basic aminoacids or their associating more than the scope of 20 amino-acid residues more than 8.Other preferred polycation and their pharmaceutical composition have description in WO 97/30721 (for example polymine) and WO99/38528.Preferably these polypeptide contain the amino-acid residue between 20 and 500, especially the residue between 30 and 200.

These polycationic compounds can chemistry or reorganization produce and maybe can derive from natural sources.

Positively charged ion (many) peptide also can be antimicrobial, just like { Ganz, T., the characteristic of summarizing among the 1999}.These (many) peptides can be protokaryon or the animal or plant source or can chemistry or reorganization generation (WO 02/13857).Peptide also can belong to defensin class (WO 02/13857).The sequence of such peptide for example finds in the antimicrobial sequence library of network address below:

http://www.bbcm.univ.trieste.it/～tossi/pag2.html

Such host defense peptide or defensive substance also are the polymeric preferred forms of polycation according to the present invention.Usually, allow as the compound that preferably activates (or downward modulation) by the dead end product of the adaptive immune system of APC (comprising dendritic cell) mediation as the polycation polymer.

Especially being preferably used as range of polycationic substances of the present invention is cathelicidin deutero-antimicrobial peptide or derivatives thereof (International Patent Application WO 02/13857; quote as a reference) herein; especially come from the antimicrobial peptide of Mammals cathelicidin, preferably from people, ox or mouse.

The polycationic compounds that comes from natural origin comprises HIV-REV or HIV-TAT (deutero-cationic peptide, feeler foot peptide, chitosan or chitin other derivative) or comes from biochemistry or these peptides or proteinic other peptide that reorganization produces.Other preferred polycationic compounds is cathelin or being correlated with or the deutero-material from cathelin.For example, mouse cathelin has aminoacid sequence NH ₂The peptide of-RLAGLLRKGGEKIGEKLKKIGOKIKNFFQKLVPQPE-COOH.Relevant or deutero-cathelin material contains all or part of of cathelin sequence that 15-20 amino-acid residue at least arranged.Derivatize can comprise that natural amino acid is by not amino acid replacement or the modification in 20 standard amino acids.In addition, more cationic residues can be introduced in such cathelin molecule.These cathelin molecules are preferably with antigen combined.These cathelin molecules are shockingly effective as the antigenic adjuvant that does not have more adjuvant to add as a result.So such cathelin molecule is possible as effective adjuvant of the vaccine preparation that is with or without more immuno-stimulating material.

The preferred range of polycationic substances of used according to the invention another is the synthetic peptide (International Patent Application WO 02/32451 is incorporated herein by reference herein) that contains at least 2 KLK groups that separated by the connexon of 3 to 7 hydrophobic amino acids.

Pharmaceutical composition of the present invention can further comprise immunostimulatory nucleic acids.Immunostimulatory nucleic acids is the nucleic acid that for example contains neutrality or artificial CpG, is derived from non-spinal animals or extends the short of nucleic acid that some base content contains the short oligonucleotide form (ODN) (for example describing among the WO 96/02555) of non-methylated cytosine-guanine dinucleotides (CpG).Perhaps, based on as the nucleic acid of the inosine for example described among the WO01/93903 and cytidine or contain the deoxidation inosine and/or the picodna of deoxyuridine residue (describe among WO 01/93905 and the PCT/EP 02/05448, quote as a reference) can preferably be used as immunostimulatory nucleic acids of the present invention herein.Preferably, also can use the mixture of different immunostimulatory nucleic acids according to the present invention.

Any polycationic compounds above-mentioned and any as previously mentioned immunostimulatory nucleic acids associating are also within the present invention.Preferably, such uniting is according to WO 01/93905, WO02/32451, and WO 01/54720, and WO 01/93903, and those that describe among WO 02/13857 and PCT/EP 02/05448 and the austrian patent application A 1924/2001 are quoted as a reference herein.

In addition or alternately, such vaccine composition can also comprise Neurologically-active compounds except hyper-immuneserum reactive antigen according to the present invention and fragment and its coding nucleic acid.Preferably, Neurologically-active compounds is the human growth factor of describing as among the WO 01/24822 for example.Also preferably, Neurologically-active compounds is united in any polycationic compounds and/or immunostimulatory nucleic acids as previously mentioned.

On the other hand, the present invention relates to pharmaceutical composition.For example such pharmaceutical composition is a vaccine described herein.Pharmaceutical composition also is to comprise any following compound or the pharmaceutical composition of its associating: according to nucleic acid molecule of the present invention, according to hyper-immuneserum reactive antigen of the present invention and fragment thereof, support according to the present invention, according to cell of the present invention, according to antibody of the present invention, according to functional nucleic acid of the present invention with according to the binding peptide of for example anti-caline of the present invention, as any agonist described herein and antagonist.Can unite the carrier that uses in non-sterile or sterile carrier or with cell, tissue or organism about any of these compound therewith and use, for example be suitable for giving the pharmaceutical carrier of research object.Such composition comprises hyper-immuneserum reactive antigen of the present invention and fragment and the drug acceptable carrier or the vehicle of medium additive for example or treatment significant quantity.Such carrier can include but not limited to physiological saline, buffering salt, glucose, water, glycerine, ethanol and their associating.Preparation should be fit to the pattern of administration.

Pharmaceutical composition can with any effectively, mode gives easily, for example comprise by in part among other, oral cavity, anus, vagina, intravenously, intraperitoneal, intramuscular, subcutaneous, the nose, in the tracheae or the giving of intradermal routes.

In treatment or as preventive, active agent for example can be used as sterile aqueous and disperses the preferred isoosmotic Injectable composition of thing to give individuality.

The perhaps composition form that can be made into for example ointment that is suitable for topical application, emulsifiable paste, washing lotion, spongaion, eye drop, [, collutory, soak into dressing and suture and smoke substance, and can contain suitable conventional additive, comprise solvent that for example sanitas, ancillary drug penetrate and the tenderizer in ointment and the emulsifiable paste.Such topical formulations can contain the consistency conventional carriers, for example the ethanol of emulsifiable paste or ointment base and lotion or oleyl alcohol.Such carrier can constitute big from about 1% to about 98% weight of formulation; More normally they will constitute and have about 80% weight of formulation altogether.

Except above-described treatment, composition of the present invention generally can be used as the wound treatment agent and combines as the replacement of antibiont prevention or with the antibiont prevention with the stroma protein that stops bacterial adhesion and expose in the wounded tissue and for the preventive use of dental treatment.

Vaccine composition is injectable form easily.Can adopt traditional adjuvant to react with enhancing immunity.The suitable unitary dose of vaccine inoculation is 0.05-5 μ g antigen/per kilogram of body weight, preferably gives such dosage 1-3 time, intermittently 1-3 week.

Shown in dosage range, compound of the present invention should not observed and hinder them to give suitable individual secondary toxic action.

Further in the embodiment, that the present invention relates to diagnose and packing medicine and test kit, the container of the above-mentioned compositions of one or more the present invention that comprised one or more fillings.Composition can or be united with useful amount, dosage, preparation and be existed.The notice of the form of government organs' regulation of manufacturing, use or the sale of relevant with such container is management medicine or biological product has reflected the approval of mechanism of manufacturing, use or the sale of the human product that gives.

Related to the present invention, any disease that for example purposes of pharmaceutical composition or vaccine is relevant more preferably suppurative disease or morbid state that links to each other with streptococcus pneumoniae or link of suis especially as disclosed herein.Relevant therewith, notice that streptococcus agalactiae has comprised some bacterial strains that comprise disclosed herein those.Relevant with the infectation of bacteria that will prevent and/or treat according to the present invention, disease that cause or that link comprises bacillary pharyngitis, otitis media, pneumonia, microbemia, meningitis, peritonitis, wound infection and septicemia except other the mankind.

Still in the further embodiment, the present invention relates to use any according to hyper-immuneserum reactive antigen of the present invention and segmental screening method thereof.Such screening method is known to those skilled in the art, can design described method with screening agonist or antagonist.Preferably screened under this situation and to have suppressed or stop according to hyper-immuneserum reactive antigen of the present invention and fragment and interacting partner bonded antagonist.Such interacting partner can be the interacting partner of natural generation or the interacting partner that non-natural takes place.

The method that the present invention also provides SCREENED COMPOUND to be to differentiate those compounds of the function that strengthens (agonist) or blocking-up (antagonist) hyper-immuneserum reactive antigen of the present invention and fragment or nucleic acid molecule, for example the interaction of itself and binding molecule.Method for screening can comprise high-throughput.

For example, in order to screen agonist or antagonist, can prepare interacting partner and nucleic acid from the cell that expression is incorporated into hyper-immuneserum reactive antigen of the present invention and segmental molecule thereof respectively according to nucleic acid molecule of the present invention, can be synthesis reaction mixture, cell chamber, the prepared product of film, cell envelope or cell walls or any they for example.Prepared product have or can not be agonist or antagonist candidate molecules in the presence of hatch with the hyper-immuneserum reactive antigen and the fragment thereof of mark.Candidate molecules and binding molecule bonded ability are reflected in the tagged ligand bonded and reduce.Free combination does not promptly cause the most likely good antagonist of molecule of hyper-immuneserum reactive antigen and the functional effect of fragment thereof.Fine bonded also causes that with hyper-immuneserum reactive antigen and fragment molecule identical or closely-related functional effect thereof be good agonist.

Can be for example activity by the back mensuration reporting system that interacts at candidate molecules and cell or suitable cellular preparations, and compare and measure the functional effect of possible agonist and antagonist with the effect of the molecule of hyper-immuneserum reactive antigen of the present invention and fragment or initiation and hyper-immuneserum reactive antigen and fragment same function thereof.Useful in this reporting system includes but not limited to that colorimetric mark substrate conversion is a product, and the change of hyper-immuneserum reactive antigen and segmental functional activity thereof is had the reporter gene of response, and known in the art in conjunction with test.

Another example of antagonist test is a competition experiments, associating hyper-immuneserum reactive antigen of the present invention and fragment thereof and the possible antagonist that membrane-bound binding molecule, reorganization binding molecule, natural substrate or part, substrate or part dummy are arranged under competition inhibition test appropriate condition.Can be for example with radioactivity or colorimetric compound mark hyper-immuneserum reactive antigen and fragment thereof, so can accurately measure the hyper-immuneserum reactive antigen that is incorporated into binding molecule or is converted into product and fragment thereof to estimate the validity of possible antagonist.

Possible antagonist comprises and is incorporated into hyper-immuneserum reactive antigen of the present invention and fragment thereof and thereby suppresses or cancel its active little organic molecule, peptide, polypeptide and antibody.Possible antagonist also can be little organic molecule, peptide, polypeptide, for example is incorporated into same loci on the binding molecule but does not cause the closely-related protein or the antibody of hyper-immuneserum reactive antigen of the present invention and segmental functionally active thereof.

Possible antagonist comprises small molecules, and it is incorporated into and has occupied hyper-immuneserum reactive antigen and segmental binding site thereof, stops thus and the combining of cell binding molecule, and makes normal biological activity be prevented from.Micromolecular example includes but not limited to little organic molecule, peptide or peptide sample molecule.

Other possible antagonist comprises that antisense molecule (, sees { Okano, people such as H., 1991} for the description of these molecules; Oligonucleotide is as the antisense inhibitor of genetic expression; CRC Press, BocaRation, FL (1988)).

Preferred possible antagonist comprises hyper-immuneserum reactive antigen of the present invention and segmental derivative thereof.

As use herein according to hyper-immuneserum reactive antigen of the present invention and segmental activity thereof be its be incorporated into the ability of any its interacting partner be incorporated into it or any interacting partner the degree of ability.

Aspect specific, the invention provides hyper-immuneserum reactive antigen of the present invention and fragment thereof, nucleic acid molecule or inhibitor disturb pathogenic agent and be responsible for infecting initial physical interaction purposes between the mammalian hosts of sequela.Especially can use molecule of the present invention aspect following: stop i) that streptococcus agalactiae adheres to mucomembranous surface with implanted device on the mammalian cell extracellular matrix protein or the extracellular matrix proteins of wound; Ii) block the bacillary adhesion between mammalian cell extracellular matrix protein and mediation tissue injury or the invading bacteria protein; Iii) cause the escape of immune defense; For example iv) block except the implantation of device or other surgical technic morbific normal procedure in the infection of the acquisition startup by suppressing nutrition.

Each dna encoding sequence provided herein can be used for the discovery and the exploitation of antimicrobial compounds.Coded protein can be as the target spot of antimicrobial drug screening when expressing.In addition, the protein amino acid region of fgs encoder or Shine-Delgarno or separately other translation of mRNA promote dna sequence dna of sequence can be used to make up the expression of antisense sequences with control purpose encoding sequence.

Can adopt antagonist and agonist for example to suppress to be derived from the suis especially disease of streptococcus agalactiae infection, for example septicemia.

Still on the other hand, the present invention relates to affine device, so affine device comprises at least a support material and is attached to any according to hyper-immuneserum reactive antigen of the present invention and fragment thereof of support material.Since according to hyper-immuneserum reactive antigen of the present invention and fragment thereof to their target cell or the specificity of target molecule or their interacting partner, as long as satisfy the bonded condition, the interacting partner that hyper-immuneserum reactive antigen and fragment thereof allow them is selective removal from the sample of any kind of that is applied to support material.Sample can be biology or medical sample, includes but not limited to fermenting broth, cell debris, cellular preparations, tissue preparation thing, organ prepared product, blood, urine, lymph liquid, liquid or the like.

Hyper-immuneserum reactive antigen and fragment thereof can be attached to matrix in mode covalently or non-covalently.Suitable support material is known for those skilled in the art, and is selected from Mierocrystalline cellulose, silicon oxide, glass, aluminium, paramagnetic beads, starch and dextran.

The present invention further illustrates by following diagram, example and sequence list, therefrom can obtain further characteristic, embodiment and advantage.Only be appreciated that and do not give this example via disclosed restriction via illustration.

Related to the present invention

Fig. 1 has shown the feature at people's antibody sources of streptococcus agalactiae.

Fig. 2 has shown the feature from the small segment genomic library LSAg-70 of streptococcus agalactiae ATCC 12403.

Fig. 3 has shown that use biotinylation human IgG is by MACS selecting bacterial cell.

Fig. 4 has shown the serotype of bacterial strain uses therefor and has adopted the example of the gene distribution analysis of differentiating one of antigen.

Fig. 5 has shown by the example with epitope specificity antibody in the intestinal bacteria lysate immune induction mouse.

Fig. 6 has shown by the example of flow cytometer with epitope specificity antiserum(antisera) staining cell surface.

Fig. 7 has shown the mensuration of the epi-position inductive antibody fungicidal activity of selecting in the in vitro tests.

Table 1A has shown the summary of all screenings of carrying out with genome streptococcus agalactiae library and human serum.Table 1B has shown the antigen protein that indicates discriminating by sequence in the proteantigen zone of listing among the table 1A.

Table 2 has shown the summary with the epi-position serological analysis of human serum.

Table 3 has shown the summary that the antigenic gene distribution differentiated in 46 streptococcus agalactiae strains is analyzed.

Table 4 has shown the summary of mouse immune originality experiment.

Table 5 has shown the summary of all screenings of carrying out with genome streptococcus agalactiae library and human serum.

Table 6 has shown the serological summary of epi-position with human serum.

Table 7 has shown the summary of mouse immune originality experiment.

In detailed description, mention be shown in below have in more detail and to describe.

Fig. 1 has shown the human serum of the anti-streptococcus agalactiae antibody of measuring as immunity test and the signature analysis of cervical discharge.Use is measured total anti-streptococcus agalactiae IgG and IgA antibody horizontal from the total bacterial lysate or the culture supernatant component of streptococcus agalactiae serotype III strain ATCC 12403 preparations by standard ELISA.(A) shown representative experimental results by health adult's serum of total bacterium crack protein matter.The serum dilution (for IgA2.000X, for IgG 10,000) that data are expressed as in the linear detection range is calculated the ELISA unit of coming in the absorbancy at 405nm place.Word figure has been pointed out to be included in the non-pregnant serum of health adult and has been merged the serum that (from 52) in the thing select.(B), the high titre serum of selecting by ELISA is carried out IDA in order to ensure multiple immunoreactivity to proteantigen.Shown and used from total bacterial lysate of streptococcus agalactiae serotype III ATCC 12403 strains preparation with in the dilution patients serum's who selects of 5.000X representative experimental results.(C) shown by IDA selecting from the pregnant woman's who does not troop cervical discharge.The IgA content of the antibody that quantitative analysis uterine cervix meche extracts.Use total bacterial lysate in porous spot equipment, to test immunoreactivity from each prepared product 2 μ g IgA.With anti-people IgA second antibody video picture spot.Select and merge the IgA prepared product that has shown with responding property of GBS protein (pointing out) by arrow.

Fig. 2 (A) has shown the distribution of the clip size of streptococcus agalactiae ATCC 12403 small segment gene library LSAg-70.Behind the clone of 576 random chooses of order-checking, repair neat sequence (464) to eliminate the carrier residue, to clone's quantity mapping of range gene group clip size is arranged.(B) clone who has shown the identical LSAg-70 one cover random sequencing on streptococcus agalactiae ATCC 12403 karyomit(e)s legend that distributes.Rectangle pointed out and the ORF paired sequence that note is arranged, rhombus represented with+/+or+/-the complete paired clone of non-coding chromosome sequence of direction.Annular has determined to have all clones' of chimeric sequences position.Digital distance in the base pair has been pointed out the direction on the annular genome.Numeral under the figure and per-cent have provided the division of various clone's set in the library.

Fig. 3 (A) has shown that the MACS with biotinylated human IgG selects.Screened the LSAg-70 library among the pMAL9.1 with the biotinylated IgG of 15-20 μ g (PSag11-IgG, purifying from human serum).As negative control, there is not serum to join the library cell of screening.Select the cell count that shown for the first time, selected after the wash-out for the second time and for the third time (go up respectively, in and figure below) for every the wheel.(B) shown the specificity clone's (1-26) that bacterium surface displaying selects reactivity, by with MACS select use at 1: 3, the IDA of 000 dilution human serum IgG merging thing (PSag11-IgG, 4 μ g/ μ l) is analyzed.As load contrast, high immunize rabbit serum 1: 5, also analyzed by 000 extent of dilution with the antibody that points to platform protein LamB for identical spot.M, the molecular wt marker; L does not have the clone's that exogenous peptide inserts extract from expressing LamB.

Fig. 4 (A) has shown the diagram that is used for the streptococcus agalactiae clinical isolates different serotypes that gene distribution researchs and analyses.Many bacterial strains can not somatotype, can represent extra serotype.(B) shown pcr analysis with the gbs0061 gene distribution of separately oligonucleotide and 46 streptococcus agalactiae strains.The size of PCR fragment prophesy is 814bp.1-46, the streptococcus agalactiae strain is as the clinical isolates that shows under at A;-, do not add genomic dna; +, the genomic dna of streptococcus agalactiae ATCC 12403 is as the template of library construction.

Fig. 5 has shown with streptococcus agalactiae deutero-epi-position being arranged and expressing Lamb or total bacterial lysate of the escherichia coli cloning of FhuA is induced the measurement of epitope specificity mice serum IgG antibody horizontal.(A) the three cover mice serums that produce with gbs0428, gbs0628, gbs632 epi-position respectively (every group of 5 mouse, 1-5) representative peptide ELISA experiment have been shown.Two different extent of dilution test sera: black post: 100X; Gray columns: 1000X.In peptide ELISA, used corresponding to the biotin labeled synthetic peptide of epi-position separately.Refer to FhuA or LamB with the intestinal bacteria lysate inductive serum that does not have streptococcus agalactiae deutero-epi-position.(B) shown the typical immunodotting experiment of using from the lysate of the individual escherichia coli cloning preparation selected for injected in mice.The intestinal bacteria lysate that serum is not carried epi-position exhausts to remove the proteinic antibody of Chinese People's Anti-Japanese Military and Political College enterobacteria.The example that has shown gbs0918, gbs0428, gbs0628 and gbs632 epi-position.Negative control (-) is the platform protein colon bacillus clone who has time.Arrow has been pointed out the position of platform protein LamB and FhuA.

Fig. 6 has shown by flow cytometer specific antibody bonded detection on the streptococcus agalactiae cell surface.Mice serum and polyclonal serum and streptococcus agalactiae strain serotype III are hatched and are analyzed with flow cytometer before the immunity of the anti-streptococcus agalactiae serotype III lysate of cultivating among Fig. 6 A.Contrast has shown the level of the non-specific binding of two anti-and streptococcus agalactiae cell surfaces.Histogram among Fig. 6 B has been pointed out to compare because the increase of the specificity combined with fluorescent of anti-gbs0031, anti-gbs1925 and anti-gbs0012 antibody with the anti-control serum that only contains the intestinal bacteria lysate of " sky " platform protein FhuA that produces.

Fig. 7 has shown as killing the fungicidal activity of the epitope specificity antibody of measuring in the test in the body.Killing of immune serum active parallelly with suitable control serum measured and calculated with respect to control serum.Data are expressed as the per-cent that kills, and are exactly the reduction as the consequence bacterium cfu of specific antibody existence.The high immune polyclone mice serum that produces with the streptococcus agalactiae lysate and from the serum of non-immune mouse respectively as the positive and the negative control of test.Test (A) gbs0012, gbs0016, gbs0031, gbs0428, gbs1306 and gbs2018 epi-position and with (B) gbs0233, gbs0419, gbs0942, gbs0975, gbs1038, the fungicidal activity of the immune serum that gbs1144 and gbs2093 epi-position produce is represented data with respect to suitable contrast, and contrast can be for example with not having streptococcus agalactiae deutero-epi-position and expressing Lamb or the escherichia coli cloning inductive serum of FhuA.Streptococcus agalactiae serotype III cell and mouse phagocytic cell were hatched 60 minutes, and the bacterium of survival is counted cfu after the blood agar and carries out quantitatively by being inoculated in.

Table 1: the immunogenic protein that bacterium surface displaying is differentiated.

(A) vertical hurdle A, the 300bp library of streptococcus agalactiae ATCC 12403 in fhuA, NSag8-IgA (826), B, the 300bp library in fhuA, PSag10-IgA (768), C, the 300bp library in fhuA, PSag10-IgG (711), D, the 300bp library in fhuA, PSag11-IgG (640), E, the 70bp library in LamB, NSag8-IgA (1057), F, the 70bp library in LamB, NSag8-IgG (869), G, the 70bp library in LamB, PSag10-IgA (904), H, the 70bp library in LamB, (493) of PSag10-IgA-absorption, I, the 70bp library in LamB, PSag10-IgG (910), J, the 70bp library in LamB, PSag11-IgA (631), K, the 70bp library in LamB, PSag11-IgG (926), L, 70bp library in LamB, PSag18-IgA (691), M, 70bp library in LamB, PSag-sIgA (628);

*, be longer than the forecast of 5 amino acid whose antigen sequences with program ANTIGENIC (Kolaskar and Tongaonkar, 1990).Table 1B has listed aminoacid sequence and has indicated the immunogenic protein of discriminating and the peptide that bacterium surface displaying is differentiated.The antigen peptide of being differentiated by bacterium surface displaying has identical copy in the protein of listing from streptococcus agalactiae.Shown peptide and a plurality of human serum reaction (seeing Table 2).So the serum of these peptides of target can be discerned a plurality of protein.

Table 2: the hematology of using the epi-position of human serum.

The synthetic peptide of individuality of the epi-position that representative is selected and the immunoreactivity of human serum have been shown.Reactive table of degree is shown+, ++ or +++, and add individual reaction with peptide and individual serum (13 patients and 9 health adults, 22 altogether).ELISA unit as all reactive add and the basis on calculate the total points of each peptide.For+mark is 2-8, ++ for 9-16 and +++be 17-26.Behind the correcting background from OD _405nmReading and serum dilution calculate ELISA unit.The amino acid of having pointed out first and last amino-acid residue in the vertical hurdle respectively from amino acid to having provided synthetic peptide according to the position in the antigen ORF of ATCC 12403 strain gene group notes.Peptide title: gbs0012.1 is present in the ORF:gbs0012 of note.

Table 3: the gene distribution of streptococcus agalactiae strain

Oligonucleotide with the gene that is specific to the coding related antigen has been tested 46 streptococcus agalactiae strains as showing among Fig. 4 A by PCR.For the amplification that confirms correct dna fragmentation is checked order to the PCR fragment that a PCR who selects reacts.*, be derived from the number of amino acid replacement in the serotype IA strain of the order-checking of comparing with streptococcus agalactiae ATCC 12403.#, the replacement bacterial strain that is used to check order is not because gene exists in serotype IA strain.

Table 4: the immunogenicity of epitope

With comprise coding be blended in the platform protein LamB of streptococcus agalactiae peptide or FhuA plasmid the escherichia coli cloning immunoassay streptococcus agalactiae immunogenicity of antigens.Use corresponding escherichia coli cloning lysate to detect and measure the existence of epi-position specific antibody by peptide ELISA and/or immunodotting.The result is expressed as+extremely ++ +++, be calculated as reactive summation (as pointing out on Fig. 5 A) for peptide ELISA based on each body mice serum of ELISA unit, (5 individualities) mice serum that is calculated as merging for immunodotting (IB) with contain the reactive intensity of the proteinic epi-position of platform (as pointing out on Fig. 5 B).The amino acid of pointing out first and last amino-acid residue in the vertical hurdle respectively from amino acid to having provided synthetic peptide according to the position in the antigen ORF of the gene annotation of ATCC 12403.

Table 5: the immunogenic protein that bacterium surface displaying is differentiated.

(A) the 300bp library of streptococcus agalactiae ATCC12403 among the fhuA, IC8-IgA (826), B, 300bp library among the fhuA, P10-IgA (768), C, 300bp library among the fhuA, P10-IgG (711), D, 300bp library among the fhuA, P11-IgG (640), E, 70bp library among the lamB, IC8-IgA (1057), F, 70bp library among the lamB, IC8-IgG (869), G, 70bp library among the lamB, P10-IgA (904), H, 70bp library among the lamB, P10-IgA-adsorbs (493), I, 70bp library among the lamB, P10-IgG (910), J, 70bp library among the lamB, P11-IgA (631), K, 70bp library among the lamB and P11-IgG (926), *, the prophesy of being longer than 5 amino acid whose antigen sequences with program ANTIGENIC (Kolaskar and Tongaonkar, 1990).

Table 6: the serology of using the epi-position of human serum.

The synthetic peptide of individuality of the epi-position that representative is selected and the immunoreactivity of human serum have been shown.Reactive table of degree is shown+, ++ or +++, add individual reaction with peptide and individual serum (13 patients and 9 health adults, totally 22).Calculate the total points of each peptide on as the basis of the total points of each peptide in ELISA unit.For+mark is 2-8, ++ be 9-16, +++be 17-30.After the background correction from OD _405nmReading and serum dilution calculate ELISA unit.The amino acid of pointing out first and last amino-acid residue in the vertical hurdle respectively from amino acid to having provided according to synthesizing the position of peptide in the antigen ORF of the gene annotation of ATCC12403.Peptide title: gbs0233.1 is present in the ORF:gbs0233 of note.

Table 7: the immunogenicity of epitope in the mouse.

With comprise coding be blended in the platform protein LamB of streptococcus agalactiae peptide or FhuA plasmid the escherichia coli cloning immunoassay streptococcus agalactiae immunogenicity of antigens.Detect and measure the existence of epi-position specific antibody by peptide ELISA.The result is expressed as+extremely ++ +++, be calculated as reactive summation (as pointing out on Fig. 5) for peptide ELISA based on each body mice serum of ELISA unit.The amino acid of pointing out first and last amino-acid residue in the vertical hurdle respectively from amino acid to having provided synthetic peptide according to the position in the antigen ORF of the gene annotation of ATCC12403.

Embodiment

Embodiment 1: based on the sign in the human serum of anti-streptococcus agalactiae antibody source and select, the preparation of antibody screening reagent

Process of the test

Enzyme linked immune assay (ELISA).

The 5-10 μ g/ml gross protein bag that is cushioned liquid (0.1M yellow soda ash pH 9.2) dilution with bag by the ELISA flat board (Maxisorb, Millipore).Make three dilution serum among the PBS-BSA.According to the recommendation of production firm (extent of dilution: 1,000x) use the anti-human IgG of high specific horse horseradish peroxidase (HRP)-connections or anti-people IgA two to resist (Southern Biotech).By based on automatic ELISA reading apparatus (TECAN SUNRISE) OD _405nmReading to measure substrate conversion be color product, quantitative immune complex.

The preparation of bacterial antigens extract

Total bacterial lysate: bacterium is grow overnight in THB (Todd-Hewitt meat soup), repeated freezing-thaw cycles cracking bacterium: hatch on dry ice/alcohol mixture up to freezing (1 minute), thaw at 37 ℃ (5 minutes) then: repeat 3 times.After this is ultrasonic and centrifugal collection supernatant (3,500rpm, 15 minutes, 4 ℃).

Culture supernatant: behind the centrifugal removal bacterium, go up cleer and peaceful 3 parts of dehydrated alcohols, with the supernatant of the bacterial cultures of ice-cold ethanol sedimentation overnight growth ,-20 ℃ of overnight incubation by mixing 1 part.Centrifugal collecting precipitate (2,600g, 15 minutes).The exsiccant granular sludge is dissolved in PBS for ELISA, or is dissolved in urea and SDS sample buffer for SDS-PAGE and immunodotting.The protein concn of Bradford test determination sample.

Immunodotting

Prepare total bacterial lysate and culture supernatant sample from the streptococcus agalactiae serotype III strain of growth in vitro.Use BioRad Mini-Protean cell electrophoresis system to separate 10 to 25 μ g gross protein/swimming lanes by SDS-PAGE, protein transduction move on to nitrocellulose filter (ECL, AmershamPharmacia).After sealing is spent the night in 5% milk, add 2, the human serum of 000x dilution, the anti-human IgG of HRPO mark are used for detecting.

Antibody is from the extraction of uterine cervix meche

Collect cervical discharge with the absorption cylinder meche (Polyfiltronics) of introducing cervical canal in the endoscopic procedure, freezing thereafter preservation is up to extraction.According to people such as Hordnes, 1998 (suppliers of sample) extract.Briefly, meche mixes with the PBS that contains proteinase inhibitor, concussion, expel liquid from the test tube that contains meche.The concentration of total IgA and IgG antibody in the mensuration extract.

The purifying antibody that is used for the genome screening

Total anti-GBS titre based on serum that uses in the screening procedure or cervical discharge merging thing is selected from patient and healthy five parts of serum organizing.By with hot inactivated serum and full cell Bacillus coli cells (DH5 α transforms with pHIE11, with identical condition that bacterium surface displaying uses under grow) hatch and remove the proteinic antibody of Chinese People's Anti-Japanese Military and Political College enterobacteria.(the super protein G that is connected and fixed Pierce) is produced the highly enriched prepared product of IgG with the protein G affinity chromatography from barren (depleted) serum that merges according to manufacturers instruction.Also use biotin labeled anti-people IgA (Southern Biotech) the purifying IgA antibody that is fixed on streptavidin-agarose (GIBCO BRL) by affinity chromatography.Measure the efficient of checking loss (depletion) and purifying with SDS-PAGE, Western spot, ELISA and protein concn.

The result

Expression in vivo that human immune system anti-streptococcus agalactiae antibody that produce and that be present in human serum is antigen protein and their immunogenic indication.These molecules are necessary for the discriminating of each isoantigen in the method for describing as the present invention, and described method is based on the anti-GBS antibody of specificity and corresponding streptococcus agalactiae peptide or protein interactions.In order to obtain associated antibodies all components (repertoires), from following source collector's serum

I. uterine cervix and anal orifice and rectal intestine GBS carry the negative healthy pregnant woman of test

II. uterine cervix and/or anal orifice and rectal intestine GBS carry the healthy pregnant woman that negative its newborn infant of test does not still have GBS (though using antibiotic prophylaxis)

III. the adult who does not have clinical disease＜45 years old

IV. inmature individual, 5 and 10 months between child, lost maternal antibody at them, because the shortage of GSB disease not have the GSB specific antibody of acquisition

Also the donor of the past two groups is collected cervical discharge in addition.The chief value of these antibody sources mainly is a secretion IgA component, and it has directly relevant as the protectiveness effector molecule on the mucomembranous surface.

It is very important to use antibody from least two different crowds of pregnant woman and unpregnancy adult to screen, because the GBS disease also influences old man and immunocompromised host adult.In the conceived study group, still have two different patient's classifications to be included in the antigen selection, the women that GBS troops is arranged and do not have those women that troop.

The antibody that is exposed in other body fluid of inductive serum and for example mucous membrane secretory product in the individuality of pathogenic agent differentiates it is crucial for antigen.Be exposed to that GBS has caused asymptomaticly trooping, current or acute or chronic infection in the past.Trooping and infecting of streptococcus agalactiae is general, exists as the autarcetic consequence antibody from former experience.The serum of the individuality that high probably titre is not trooped contains functional antibodies, can eliminate and carry.Have only antibody to continue some antibody that existence just might be induced anti-GBS component in the identical time.For that reason, also comprise coming the serum of own individuality of trooping.Shown troop related with capsular polysaccharide (CPS)-specific antibody reacting phase.Yet whether enough the antibody capable to CPS of unclear level stops trooping of GBS, because there is the women who troops that high and low-level anti-CPS antibody are arranged, this also is genuine for the pregnant woman who does not troop.

Yet, have report to point out that the effector function and the avidity of the antibody that gestation time produces may change.Recognize that the most healthy adult is protected that to avoid invading GSB disease and susceptibility low very more important than newborn infant and old man.As if especially valuable from these individual antibody for the discriminating of corresponding antigens.Known anti-GBS antibody horizontal is along with the age increases.

GBS is the mucosal disease substance, should induce the IgA reaction; For that reason is carried out based on the screening of IgA and very important based on the screening of IgG.The fact of some streptococcus agalactiae strains expression high-affinity IgA bind receptors is also pointed out the importance of IgA in the host response.There is report to point out that not only IgG but also IgA serum antibody can and promote opsonization { Phillips-Quagliata, people such as J., 2000} by the identification of the FcRIII acceptor of PMN recently; { Shibuya, people such as A., 2000}.The main effect of IgA antibody is main neutralization at mucomembranous surface.The serum IgA level has reflected quality, quantity and the specificity of dimeric form secretion IgA.For that reason the serum gleanings is not only analyzed streptococcus IgG, also analyze the IgA level.ELISA test camber specificity second reagent is used for the antibody from the high-affinity type of test example such as IgG and IgA, but does not avoid IgM.The IgM production of antibodies takes place in elementary adaptation humoral response process, obtained low-affinity antibody, and IgG and IgA antibody has experienced the maturation of avidity, and is worth higher in opposing or preventing disease.

By a series of immunity tests to from pregnant woman's 127 serum samples and 97 one's share of expenses for a joint undertaking cervical secretionses with carry out the signature analysis of anti-streptococcus agalactiae antibody from 50 parts of serum of health adult.Use for example total bacterial extract and finish main signature analysis by ELISA from proteinic two the different antigen prepd things of culture supernatant of streptococcus agalactiae serotype III ATCC 12403 strains preparation.Shown the model experiment that uses from the group's of health adult serum among Figure 1A.In reaction is that linear given extent of dilution compares antibody titers.Reactivity based on IgG and anti-two the complex antigen mixtures (comprising serotype specificity III type pod membrane) of IgA is carried out classification to serum, selects the highest serum and carries out further immunodotting test.This is analyzed and confirms the anti-proteinic high antibody response of a plurality of GBS of the serum of selecting in advance, when especially comparing with the low titre serum of not selecting (Figure 1B).Yet, the ELISA classification of serum not always with immunodotting signal correction, to point out anti-capsular antibody be volume, controlled the ELISA reactivity of anti-total bacterial extract.Thereby, be included in antibody and merge finally selecting of serum in the thing mainly based on a plurality of immunogenicity bands in the immunodot test.This widely the antibody characteristic analysis method caused the clear and definite discriminating of anti-GBS hyper-immuneserum.

Measure the IgA content of 97 one's share of expenses for a joint undertaking cervical secretionses, tested the anti-GBS reactivity of same amount (2 μ g) by immunodotting.The serum that the positive is selected is divided into having the IgA that troops and do not troop to merge thing and being used for bacterium surface displaying dividually tests.

For bacterium surface displaying to antigenic discriminating, select and merge 5 parts of serum from two donor groups.The serum of selecting be included in four pregnant woman's merging thing (PSAg10-IgG ,-IgA, PSAg11-IgG, PSAg18-IgG and PSAg-sIgA) and the merging thing of (non-pregnant) health adult (NSAg8-IgG ,-IgA) in.By serum IgG purification and the IgA antibody of affinity chromatography, and exhaust the intestinal bacteria reactive antibody to avoid the background in the bacterium surface displaying screening from merging.

Embodiment 2: the generation of the genome dna library of the height random of streptococcus agalactiae, that select framework, small segment

Experimentation

The preparation of suis genomic dna.From the streptococcus agalactiae ATCC12403 microbionation of freezing stab culture in 50ml Todd-Hewitt broth culture and ventilate and 37 ℃ rock 18h and grow.Collect culture then, 1, centrifugal 15 minutes of 600xg removes supernatant.Clean the bacterium bead 3 times with PBS, carefully be suspended in the lytic enzyme solution (100mg/ml) of 0.5ml.Add the heat treated RNase A of 10mg/ml of 0.1ml and the RNase T1 of 20U, the careful mixing, solution is hatched 1h at 37 ℃.0.2ml 20%SDS solution and the adding of 0.1ml Proteinase K (10mg/ml) after, test tube is 55 ℃ of overnight incubation.The saturated NaCl that adds 1/3 volume then, solution was hatched 20 minutes at 4 ℃.(13,000rpm), supernatant changes new test tube over to precipitate extract in little whizzer.Use PhOH/CHCl ₃/ IAA (25: 24: 1) and use CHCl ₃/ IAA (24: 1) extraction solution.The Virahol of adding 0.6x volume is deposit D NA at room temperature, twines DNA and changes over to from solution with aseptic Pasteur transfer pipet and contain the 80% ice-cold alcoholic acid test tube.10-12, the 000xg centrifugal sediment regains DNA, then air drying and be dissolved in ddH ₂Among the O.

The preparation of minigene group dna fragmentation.Use cup-shaped pipe (cup-horn) ultrasonic apparatus (BandelinSonoplus UV 2200 ultrasonic apparatus, BB5 cup-shaped pipe is housed, exports 10 pulse per second (PPS)s at 100% power) the genomic DNA fragment mechanical shear is cut into the fragment between magnitude range 150 and the 300bp or handles (Novagen) by gentle DNase I cut into fragment between the size 50 to 70bp.Observe when DNA is broken into the fragment of 150-300bp magnitude range, ultrasonicly obtained compact a lot of fragment size distribution.Yet,, can not efficient and repeatably obtain the reduction of clip size subsequently though DNA is exposed to ultrasonic wave inductive hydromeehanics shearing force in a large number.So, use the air gun (shotgun) of Novagen to shear test kit obtains size 50 to 70bp by gentle DNase I processing fragment.1: 20 dilution DNase I that the preparation test kit provides is at MnCl ₂Exist down and in the 60Al volume, digest 5 minutes to guarantee the two strands shearing of enzyme at 20 ℃.With 2 μ l 0.5M EDTA stopped reactions, on the 2%TAE-sepharose, estimate the efficient of fragmentation.This processing has caused genomic DNA fragment to turn to nearly 50-70bp fragment.Use T4DNA polysaccharase flush end in the presence of 100 each dNTP of μ M to handle twice of (blunt-ended) fragment then to guarantee terminal effective purification (flushing).Fragment is promptly used or is frozen in-20 ℃ for subsequently use in ligation neutrality.

The description of carrier.{ Skerra, A., 1994} carrier construction pMAL4.31 on the pASK-IBA skeleton that β-Nei Xiananmei (b1a) gene that exchanges with kalamycin resistance gene is arranged.In addition, a plurality of cloning sites are advanced in the b1a gene clone.The sequence of the β-Nei Xiananmei of encoding mature prior to the leading peptide sequence of ompA to allow effectively to stride the secretion of cytoplasmic membrane.In addition, the sequence of initial 12 amino acid of the β-Nei Xiananmei of encoding mature (spacer sequence) is followed in ompA leading peptide sequence back to avoid being right after the fusion of the sequence after guide's peptase cleavage site, because for example this regional positivity electric charge amino acid group will reduce or cancel the transposition { Kajava that strides cytoplasmic membrane, A. wait people, 2000}.The SmaI restriction site is supplied with the insertion in library.Be used to select segmental FseI site, upstream that regains and NotI site, downstream both sides in the SmaI site.Read frame with the b1a genetic transcription-1 and after the sequence of 12 amino acid spacer sequences of coding, insert three restriction sites in the mode that obtains a termination codon 15bp behind the NotI site.To such an extent as to+1bp insertion has been rebuild b1a ORF and has been obtained the acquisition that β-Nei Xiananmei is accompanied by the Ampicillin Trihydrate resistance.

By the lamB gene clone being advanced a plurality of cloning site carrier construction pMAL9.1{Hashemzadeh-Bonehi of pEH1, people such as L., 1998}.Subsequently, sequence is inserted lamB in amino acid/11 54 backs, contains restriction site FseI, SmaI and NotI.Obtain the continuous reading frame of fhuA and this mode of inserting separately makes up the reading frame of this insertion with the transfer of the dna fragmentation of having selected framework, described dna fragmentation is sheared by using from the FseI of plasmid pMAL4.31 and the digestion of NotI.

Made up carrier pHIE11 by a plurality of cloning sites that the fhuA gene clone advanced pEH1.Afterwards, sequence is inserted fhuA in amino acid 405 backs, contains restriction site FseI, XbaI and NotI.Obtain the continuous reading frame of lamB and this mode of inserting is separately selected the reading frame of this insertion with the transfer of the dna fragmentation of having selected framework, described dna fragmentation is sheared by using from the FseI of plasmid pMAL4.31 and the digestion of NotI.

Be used for the clone and the evaluation in the library that framework selects.Genome streptococcus agalactiae dna fragmentation is connected to the SmaI site of carrier pMAL4.31.The recombinant DNA electroporation is advanced the competent Bacillus coli cells (GIBCO BRL) of DH10B electroporation, and transformant is inoculated on the LB agar plate that has replenished kantlex (50 μ g/ml) and Ampicillin Trihydrate (50 μ g/ml).Dull and stereotyped 37 ℃ of overnight incubation, for large-scale DNA extraction is collected the clone.Store and keep representational flat board and clone pcr analysis and the large-scale order-checking that is used for bacterium colony to collect.Simple bacterium colony PCR test is used for initial fragment size distribution and the insertion efficient of measuring roughly.From the accurate clip size of data evaluation of order-checking, in the integrity of the joint that inserts the site and the accuracy (3n+1 rule) of framework selection.

Be used for the clone and the evaluation in the library of bacterium surface displaying.From pMAL4.31 carrier cutting genomic DNA fragment, contain the streptococcus agalactiae library of restricted property enzyme FseI and NotI.Complete then segment group is transferred among plasmid pMAL9.1 (LamB) or the pHIE11 (FhuA), and described plasmid is with FseI and NotI digestion.Use identification 8bp to be rich in these two Restriction Enzymes of GC sequence, in each platform carrier, keep the reading frame of selecting in the pMAL4.31 carrier.By electroporation plasmid library is transformed into e.colidh5 then.Cell inoculation on the big LB agar plate that has replenished 50 μ g/ml kantlex, in the density that can obtain apparent single bacterium colony 37 ℃ of grow overnight.Surface from these flat boards scrapes cell then, cleans with fresh LB substratum, is divided in-80 ℃ of storages and is used for library screening.

The result

Be used for the library that framework is selected.Producing in the pMAL4.31 carrier has about 70 and two libraries (LSAg-70 and LSAg-300) of 300bp size respectively.For each library, the genome streptococcus agalactiae DNA of the pMAL4.31 plasmid DNA of about 1 μ g and the fragmentation of 50ng be connected and thereafter conversion has obtained 4x10 after framework is selected ⁵To 2x10 ⁶Individual clone.For the randomness of evaluation libraries, about 576 clones that select have at random checked order.Analysis of biological information shows that the clone who has only among these clones seldom is more than once existing.In addition, the size that shows about 80% clone has the about 40bp of mean size (Fig. 2) between scope 25 and 100bp.Nearly all sequence is followed the 3n+1 rule, has shown that all clone Selection Framework rightly.

The bacterium surface displaying library.The displaying of peptide requires to select carrier pMAL4.31 from the insertion body in LSAg-70 and LSAg-300 library from framework and transfers to showing plasmid pMAL9.1 (LamB) or pHIE11 (FhuA) on the intestinal bacteria surface.The restricted shearing genomic DNA fragment of FseI and NotI, 5ng insert body and are connected with 0.1 μ g plasmid DNA, transform DH5 α cell thereafter and have obtained 2-5x10 ⁶Individual clone.Scrape clone and freezing and not further amplification from the LB flat board.

Embodiment 3: the genomic library of use bacterium surface displaying and the hyperimmunization originality peptide sequence of human serum are from the discriminating of streptococcus agalactiae

Process of the test

The MACS screening.About 2.5x10 from given library ⁸Individual cell replenished in the LB substratum of 50 μ g/ml kantlex growth 2 hours at 37 ℃ at 5ml.1mM IPTG adds 30 minutes abduction deliverings.Clean cell twice with fresh LB substratum, approximately 2x10 ⁷Individual cell is suspended in the 100 μ lLB substratum again and transfers in the Eppendorf pipe.

The biotinylated human IgG from the serum purifying of 10 to 20 μ g joins cell, and suspension slightly jolts overnight incubation at 4 ℃.Add 900 μ l LB substratum, mixing suspension, subsequently 6,4 ℃ of 000rpm centrifugal 10 minutes (for the IgA screening, use the IgA of 10 μ g purifying, these resist with biotinylated anti-human IgG two catches).Clean cell once with 1ml LB, be suspended in 100 μ lLB substratum then again.Add 10 μ l and be coupled to the MACS microballon (Miltenyi Biotech, Germany) of streptavidin, continue to hatch 20 minutes at 4 ℃.Add 900 μ l LB substratum afterwards, sample on the MACS microballon cell suspending liquid is fixed in the MS post (Miltenyi Biotech, Germany) of magnet to counter-balanced.(the MS post also cleans balance twice with 2ml LB substratum by cleaning once with 1ml 70%EtOH).

Clean post three times with 3ml LB substratum then.After magnet is removed, by cleaning the wash-out cell with 2ml LB substratum.Behind 3ml LB substratum cleaning post, the 2ml eluate is gone up sample for the second time to identical post, repeated washing and elution step.Carry out going up sample, cleaning and elution step for the third time, obtain the whole eluate of 2ml.

Second and third round screening carry out as follows.The cell of the whole eluate of centrifugal collection is suspended in the LB substratum that 1ml has replenished 50 μ g/ml kantlex again.Culture was hatched 90 minutes at 37 ℃, induced 30 minutes at 1mM IPTG then.Collecting cell cleans once with 1ml LB substratum subsequently, is suspended in 10 μ l LB substratum.Add the biotinylated IgG of 10 to 20 μ g people once more, suspension slightly jolts overnight incubation at 4 ℃.All further steps and the first round are selected just the same.The cell inoculation that two-wheeled is selected after selecting on the LB agar plate that has replenished 50 μ g/ml kantlex, 37 ℃ of overnight incubation.

By order-checking and Western spot analysis to the evaluation of selected clone.The 37 ℃ of grow overnight of selecting that are cloned in the 3ml LB substratum that has replenished 50 μ g/ml kantlex prepare plasmid DNA to use standard program.Check order at MWG (Germany) or with TIGR (U.S.) cooperation.

For the Western spot analysis, separate about 10 to 20 μ g total cell protein matter with 10%SDS-PAGE, be transferred on the HybondC film (Amersham Pharmacia Biotech, England).End user's serum as first antibody at about 1: 3,000 to 1: 5,000 extent of dilution and be coupled to the anti-human IgG of HRP or IgA antibody as second antibody at 1: 5,000 extent of dilution detects LamB or FhuA fused protein.Use ECL detection kit (Amersham Pharmacia Biotech, Britain) to detect.Perhaps, anti-FhuA of rabbit or the anti-LamB polyclone of rabbit immune serum are as first antibody, and the associating second antibody that is coupled to HRP separately is to detect fused protein.

The result

Use biotinylation IgG to pass through magnet activated cell sorting (MACS) to the bacterium surface displaying library screening.With from health adult (NSag8-IgG ,-IgA) or P10, LSag-70 the biotinylated human IgG of the serum pref of 11,18 (see embodiment 1: antibody is from the preparations of human serum) and the merging thing of the IgA screening pMAL9.1 and the Lsag-70 library among the pHIE11.As test under the process process of selecting with describing.Fig. 3 A has shown the representative example with the screening of LSag-70 library and PSag11-IgG.As seeing ground from enumeration, after circulation is selected in first of MACS screening, the total cellular score that regains at last is dramatically from 2x10 ⁷Individual cell drops to about 2x10 ⁴Individual cell does not still have the reduction (Fig. 3 A) that has shown similar cell count of selecting of antibody adding.So carry out second and third round select.Last in third round regains about 10 with PSag11-IgG ⁴Individual cell, however when the IgG that do not have to add from human serum, only regained 2x10 ³Individual cell has clearly illustrated to select to depend on the streptococcus agalactiae specific antibody.In order to estimate the performance of screening, select 26 clones that select at random, and carry out IDA (Fig. 3 B) with screening property IgG merging thing.This analysis disclosed more than 80% clone who selects shown with relevant serum in the reactivity of the antibody that exists, and the contrast strain of expressing the LamB that does not have streptococcus agalactiae specificity inset is not reacted with identical serum.Generally, observe the speed of reaction in 35% to 90% scope.The bacterium colony pcr analysis has shown that all clones that select contain the inset of expecting magnitude range.

A large amount of subsequently clones' that select at random (the every wheel screened 600 to 1200) order-checking has caused gene and the corresponding peptide of the human serum antibodies specific identification that is used to screen or the discriminating of protein sequence.Frequency to the small part of selecting specificity clone has reflected at the serum that is used for selecting and has discerned the abundance and/or the avidity of the specific antibody of the epi-position that this clone presents.At this point, very shockingly be derived from the clone of some ORF selected more than 100 times, point out the characteristic that their hyperimmunizations are former.Table 1 and table 5 have been summed up all 13 resulting data of the screening of carrying out.Use single clone's full cell extract to verify that by IDA all clones that present in table 1 and the table 5 are to show the reactivity that shows that merges thing with the human serum that is used for each self-sizing.As seeing ground from table 1 and table 5, unique zone of the ORF of discriminating is differentiated to immunogenic, because the different big or small fragments of protein are illustrated in the surface by platform protein.

Merit attention more genes encoding that the screening of most bacterium surface displayings differentiates be attached to streptococcus agalactiae the surface and/or excretory protein.This is consistent with effect surface attachment or that excretory protein is expected in the streptococcus agalactiae virulence.

Embodiment 4: hyperimmunization originality peptide sequence and the reactive evaluation of individual human serum

Experimentation

Peptide is synthetic

Use Syroll synthesizer (Multisyntech, Witten, Germany) (PepChem, T ü bingen, Germany) on the Rink amide resins to use standard F-moc chemistry (4mg resin on a small scale; Parallel totally 288) synthetic peptide.After the combined sequence, with Fmoc-epsilon-amino caproic acid (as linking agent) and vitamin H (Sigma, St.Louis, MO; Resemble that normal amino acid is activated) the prolongation peptide.Peptide was scaled off from resin in 1 hour with 93%TFA, 5% triethyl silicane and 2% water.Dry peptide under the vacuum is from acetonitrile/water (1: 1) lyophilize 3 times.The existence of correct quality by mass spectrum at Reflex

Enzyme linked immunological experiment (ELISA)

Specification sheets according to production firm is gone up at 10 μ g/ml concentration bags by biotin labeled peptide (at the N-end) at streptavidin ELISA flat board (EXICON).According to the recommendation of production firm use the anti-human IgG two anti-(SouthernBiotech) that high degree of specificity horse horseradish peroxidase (HRP) connects (extent of dilution: 1,000x).In 200X and 1, two serum dilution test sera of 000X.After the manual bag quilt, (GENIOS, Gemini 160 ELISA robots (TECAN) TECAN) handle and analyze the peptide flat board by built-in ELISA reading apparatus is arranged.

The result

After the analysis of biological information to selected clone, design and synthesize corresponding peptide.Under situation about having, make overlapping peptide more than the epi-position of 26 amino-acid residues.All peptides synthesize with the terminal biotin labeling of N-and are used as bag on the ELISA flat board of streptavidin bag quilt by reagent.

The peptide of selecting based on the reactivity of they and individual serum is analyzed, and described peptide is included in to be used for preparing for the IgG of bacterium surface displaying and the serum of IgA screening reagent and merges thing.Shown the summary of the serum reactivity of 74 peptides in table 2 and the table 6, described peptide has been represented 22 parts of different streptococcus agalactiae antigen proteins from the genome screening that are used for human serum (being exposed to high titre individuality with 9 health from 13 patients) analysis of antigen discriminating of 55 usefulness.Mark by each peptide of calculating based on the degree of positive serum number and reaction carries out peptide.Peptide is having very high reactivity to having in reactive widely scope to weak positive serum.

Embodiment 5: use the proteinic gene distribution research of differentiating from streptococcus agalactiae of high immunogenicity

Experimentation

The antigenic gene distribution of GBS that PCR measures

The ideal vaccine antigen can be the antigen that is present in the target organism strain that all or most vaccine point to.In order to establish whether ubiquity in the streptococcus agalactiae strain of the antigenic gene of streptococcus agalactiae that coding differentiates, on a series of independently streptococcus agalactiae isolates, carry out PCR with the primer that is specific to goal gene.Obtain the isolate of streptococcus agalactiae, comprise the serotype of frequent existence in the patient as showing among Fig. 4 A.Designed oligonucleotide sequence as primer for the ORF of all discriminatings, produced approximately 1, the product of 000bp if possible comprises the immunogenicity epi-position of all discriminatings.The genomic dna of ground all streptococcus agalactiae strains of preparation is described for 2 times as embodiment.Specification sheets (Invitrogen, Holland) according to production firm uses Taq polysaccharase (1U), each oligonucleotide of 200nM dNTPs, 10pMol and test kit to advance middle performing PCR at the reaction volume of 25 μ l.As standard, carry out 30 circulations (1x:5min.95 ℃, 30x:30sec.95 ℃, 30sec.56 ℃, 30sec.72 ℃, 1x4min.72 ℃), unless that each condition must be suitable for individual primer is right.

The result

The gene of having tested the coding immunogenic protein of differentiating by PCR is they exist (Fig. 4 A) in 46 different streptococcus agalactiae strains.As an example, Fig. 4 B has shown the PCR reaction with the GBS0061 of all 46 bacterial strains pointing out.Clearly visible ground, gene is present in the bacterial strain of all analyses.PCR fragment from IA type bacterial strain is checked order, show that with the relatively more all 657bp of streptococcus agalactiae ATCC12403 strain be identical, point out high-caliber conservative property between two isolates.

From the gene of 117 analyses altogether, be present in more than 100 in the bacterial strain of all or nearly all test, and have only 5 genes in 46 bacterial strains, not have (table 3) more than 10% test.In addition, it is big or small different to have only minority gene (for example GBS0016, GBS1087, GBS1528 and GBS2018) to show, but is present in all or the most of strains separation thing.The segmental order-checking of PCR that produces from bacterial strain and subsequently and the amplification that has relatively confirmed correct dna fragmentation III type strain ATCC 12403 have disclosed the degree as the sequence difference of pointing out in the table 3.Importantly, a lot of antigens of differentiating are high conservatives in sequence and size in all bacterial strains, so be the new generation vaccine candidate of prevention GBS infection.

Embodiment 6: derive from and be used for the signature analysis of immune serum of hyperimmunization originality proteins/peptides immune mouse of the streptococcus agalactiae of showing on the intestinal bacteria surface

Experimentation

The generation of mouse immune serum

The escherichia coli cloning of the proteinic plasmid of platform that comprising has encoded is blended in the streptococcus agalactiae peptide is grown in 37 ℃ of LB substratum that replenishing 50 μ g/ml kantlex.The culture that spends the night 1: 10 dilution, growth is up to 0.5 OD ₆₀₀, hatched 2 hours with 0.2mM IPTG.Precipitate granulated bacterial cell and be suspended in the PBS damping fluid, break with ultrasonic on ice, produce the granular cell extract.According to OD ₆₀₀Measure, corresponding to 5x10 ⁷The branch loading amount intravenous injection of individual cell is appending after 2 weeks to the NMRI mouse afterwards.Inject for the second time the back blood collecting of 1 week.Peptide ELISA measures the level of epi-position specific antibody.

Antigenic vivoexpression

The antigenic expression of the streptococcus agalactiae III serotype by immunodotting test body outgrowth.Test different growth mediums and culture condition to detect antigenic existence the in total lysate and the microbial culture supernatant.When detecting, think that expression has been proved corresponding to the specific band point of molecular wt of prophesy and electrophoretic mobility.

Cell surface dyeing

Followingly carry out the analysis of cell streaming instrument.Bacterium grows under culture condition, obtains the antigenic expression that shows as IDA.Cell cleans twice in Hanks balanced salt solution (HBSS), cell density is adjusted into 100 μ l HBSS, about 1X10 among the 0.5%BSA ⁶Individual CFU.4 ℃ hatch 30 to 60 minutes with 50 to 100 times mouse resisting anteserums of dilution after, by at excessive HBSS, centrifugal among the 0.5%BSA washed unconjugated antibody off.Second goat anti-mouse antibody of fluorescein (FITC) mark (F (ab ') ₂Fragments specific) hatched 30 to 60 minutes at 4 ℃ with cell.After the cleaning, cell is fixed with 2% Paraformaldehyde 96.Use Becton DickinsonFACScan flow cytometer to detect bonded antibody, with the further analytical data of computer program CELLQuest.Negative control sera comprises the mouse polyclonal serum that lysate produced that preceding serum of mouse immune and the IPTG inductive Bacillus coli cells that transforms with plasmid-encoded gene lamB that does not have streptococcus agalactiae genome inset or fhuA prepare.

Sterilization (killing) test

Mouse macrophage (RAW246.7 or P388.D1) and bacterium hatch, and measure losing of the bacterium that lives after 60 minutes by enumeration.In brief, bacterium cleans twice with Hanks balanced salt solution (HBSS), and cell density is adjusted into about 1X10 among the 50 μ l HBSS ⁵Individual CFU.Bacterium and mice serum (can reach 25% at most) and GPC (can reach 5% at most) in cumulative volume 100 μ l 4 ℃ hatched 60 minutes.The bacterium of preconditioned and scavenger cell (mouse cell line RAW264.7 or P388.D1; Per 100 μ l 2X10 ⁶Individual cell) with 1: 20 mixed, rotation jolts on the device hatches for 37 ℃ at 500rpm.The aliquot of each sample dilutes with sterilized water, and incubated at room 5 minutes is with the cracking scavenger cell.Then the serial dilution thing is inoculated on the Todd-Hewitt bouillon agar flat board.Dull and stereotyped 37 ℃ of overnight incubation, with Countermat glistening colony calculating instrument (IUL instrument) counting bacterium colony.Control serum comprises the mouse polyclonal serum that lysate produced that serum before the mouse immune and the IPTG inductive intestinal bacteria that transform with the plasmid that comprises the gene lamB that do not have streptococcus agalactiae genome inset or fhuA prepare.

The result

Immunogenicity in mice

Use as among Fig. 5 A and the B respectively for example and the expression of summing up in table 4 and the table 7 intestinal bacteria that are embedded in given epi-position in LamB or the FhuA platform protein pass through the existence of peptide ELISA and/or immune spot measuring specific antibody.43 novel GBS antigens that 61 different epitope regions present in mouse, show be have immunogenic.Use the total bacterial lysate and the culture supernatant of streptococcus agalactiae serotype III strain preparation to pass through IDA positive serum (data not shown) then.If this is analyzed as measuring whether antigen protein expresses and in order to estimate the first step of surface expression under what growth conditions of polypeptide with facs analysis.Do not expect that based on the data of document all protein can express under conditions in vitro.

The cell surface dyeing of streptococcus agalactiae.Cross based on the accessible cell surface of some antigen proteins of evidence of flow cytometer logical subsequently.The anti-streptococcus agalactiae lysate of GBS cell and preimmune serum and generation or comprised the polyclone mice serum of escherichia coli cloning that coding is blended in the proteinic plasmid of platform of streptococcus agalactiae peptide and hatch is afterwards with fluorescently-labeled two anti-detections.As showing ground among Fig. 6 A, the remarkable migration by streptococcus agalactiae III serotype cell mass fluorescence proves that the antiserum(antisera) of the anti-streptococcus agalactiae lysate of production contains the antibody of anti-surface component.The streptococcus agalactiae III serotype cell of similar cell surface observe to(for) the polyclonal serum of the antigenic peptide of GBS of anti-many discriminatings of producing dye (Fig. 6 B).In some examples, point out the not dyeing (Fig. 6 B) of ground sub-fraction bacterium as detected two peaks in the histogram.This phenomenon can be the result that the antibody-binding fraction that causes of gene product differential expression in the bacteria growth process, insufficient antibody horizontal or other surface molecular or plasma proteins suppresses.Importantly, to be proved to be in this test also be male for well-known protectiveness GBS antigen Sip/gbs0031.

Body outer disinfecting activity.The death of conditioning phagocytosis is for example milestone of the host defense of streptococcus agalactiae of the outer bacterium of anti-cell.If specific antigen inductive antibody capable is in conjunction with activated complement component (C3bi), antibody is kill (sterilizations) that opsonize and induce phagocyte (scavenger cell and neutrophil leucocyte) to the cell surface combination of bacterial antigens.Data have presented the fungicidal activity of measuring by the antigen-specific antibodies that produces with corresponding antigens among Fig. 7 in mouse.According to these data, some novel GBS antigens, gbs0012 for example, gbs0016, gbs0428, gbs1306 and gbs2018, inducing function antibody.Importantly, well-known protectiveness GBS antigen Sip/gbs0031 is proved to be strong positive in identical test.

These experiment confirms the prophesy of bioinformation, a lot of protein are because their signal peptide sequence and being transported has shown that in addition they are present on the cell surface of streptococcus agalactiae III serotype.They confirm that also these protein can be used for having the identification of people's antibody of functional performance, make them become the of great value candidate of vaccine of the anti-GBS disease of exploitation.

Table 1A: the immunogenic protein that bacterium surface displaying is differentiated.

The streptococcus agalactiae antigen protein	The function of inferring (passing through homology)	The immunogenicity amino acid * * that infers	Each ORF and the clone that selects of screening number	The position (amino acid) in the immunogenicity zone of differentiating	Seq. ID (DNA, protein)
The streptococcus agalactiae antigen protein	The function of inferring (passing through homology)	The immunogenicity amino acid * * that infers	Each ORF and the clone that selects of screening number		Seq. ID (DNA, protein)	gbs0012	Be similar to β-Nei Xiananmei a little	4-20，35-44，65-70，73-87，92-98，112-137，1 52-161，177-186，193-200，206-213，229-255 ，282-294，308-313，320-326，349-355，373-3 84，388-406，420-425	B：13，C：6	115-199	1，218
gbs0016	Glucan-binding protein matter B	5-24，35-41，44-70，73-89，103-109，127-143 ，155-161，185-190，192-207，212-219，246-2 62，304-336，372-382，384-393，398-407，412 -418，438-444	B：12，C：4， D：3，E：5， H：4，I：12， M：2	1-75， 76-161， 164-239	2，219	gbs0012	Be similar to β-Nei Xiananmei a little		B：13，C：6	115-199	1，218
gbs0016	Glucan-binding protein matter B		B：12，C：4， D：3，E：5， H：4，I：12， M：2	1-75， 76-161， 164-239	2，219	gbs0024	Ribose phosphoric acid formyl G-NH2 synthase	4-10，16-58，60-71，77-92，100-126，132-146 ，149-164，166-172，190-209，214-220，223-2 29，241-256，297-312，314-319，337-343，351 -359，378-387，398-418，421-428，430-437，4 40-448，462-471，510-519，525-536，552-559 ，561-568，573-582，596-602，608-630，637-6 49，651-665，681-702，714-732，739-745，757 -778，790-805，807-815，821-829，836-842，8 46-873，880-903，908-914，916-923，931-940 ，943-948，956-970，975-986，996-1015，1031 -1040，1051-1069，1072-1095，1114-1119，11	F：4	802-812	3，220

The streptococcus agalactiae antigen protein	The function of inferring (passing through homology)	The immunogenicity amino acid * * that infers	Each ORF and the clone that selects of screening number	The position (amino acid) in the immunogenicity zone of differentiating	Seq. ID (DNA, protein)
The streptococcus agalactiae antigen protein	The function of inferring (passing through homology)	The immunogenicity amino acid * * that infers	Each ORF and the clone that selects of screening number		Seq. ID (DNA, protein)			30-1148，1150-1157，1169-1176，1229-1238
gbs0031	The surface immunogenic protein	5-12，14-26，35-47，52-67，72-78，83-98，121 -141，152-159，163-183，186-207，209-257，2 64-277，282-299，301-309，312-318，324-339 ，358-368，372-378，387-397，425-431	A：17， B：53， C：36，D：4	46-291	4，221			30-1148，1150-1157，1169-1176，1229-1238
gbs0031	The surface immunogenic protein		A：17， B：53， C：36，D：4	46-291	4，221	gbs0048	Hypothetical protein matter	29-38，44-64，70-76，78-87，94-100，102-112 ，119-134，140-149，163-173，178-186，188-1 94，207-234，247-262，269-290	K：13	73-92	5，222
gbs0053	Aldehyde-alcoholdehydrogenase (adhE)	10-28，36-63，77-87，103-119，127-136，141- 169，171-183，195-200，207-232，236-246，25 1-265，268-283，287-297，314-322，335-343， 354-363，384-390，405-411，419-436，443-45 5，467-473，480-513，518-529，550-557，565- 585，602-608，616-625，632-660，665-677，68 5-701，726-736，738-747，752-761，785-796， 801-813，838-853，866-871	E：4	757-774	6，223	gbs0048	Hypothetical protein matter		K：13	73-92	5，222
gbs0053	Aldehyde-alcoholdehydrogenase (adhE)		E：4	757-774	6，223	gbs0061	Rp1B ribosomal protein L2	31-38，61-66，74-81，90-115，123-145，154-1 67，169-179，182-193，200-206，238-244，267 -272	F：2，I：12	235-251	7，224
gbs0084	The RNA polymerase (α subunit) that DNA instructs (rpoA)	19-25，38-54，56-64，66-72，74-92，94-100，1 16-129，143-149，156-183，204-232，253-266 ，269-275，294-307	C：4，D：6	241-313	8，225	gbs0061	Rp1B ribosomal protein L2		F：2，I：12	235-251	7，224
gbs0084	The RNA polymerase (α subunit) that DNA instructs (rpoA)		C：4，D：6	241-313	8，225	gbs0107	Conservative hypothetical protein matter	5-34，50-56，60-65，74-85，89-97，108-119，1 59-165，181-199，209-225，230-240，245-251 ，257-262，274-282，300-305	K：2	64-75	9，226
gbs0108	Deoxyuridine 5 '-triphosphopyridine nucleotide lytic enzyme	5-13，16-21，27-42，45-52，58-66，74-87，108 -114，119-131	I：5	39-51	10，227	gbs0107	Conservative hypothetical protein matter		K：2	64-75	9，226
gbs0108	Deoxyuridine 5 '-triphosphopyridine nucleotide lytic enzyme	5-13，16-21，27-42，45-52，58-66，74-87，108 -114，119-131	I：5	39-51	10，227	gbs0113	Ribose abc transport albumen	6-23，46-54，59-65，78-84，100-120，128-133 ，140-146，159-165，171-183，190-204，224-2 32，240-248，250-259，274-280，288-296，306 -315	F：4	267-274	11，228
gbs0123	Be similar to the argininosuccinic acid synthase	5-12，15-24，26-36，42-65，68-80，82-104，11 1-116，125-144，159-167，184-189，209-218， 235-243，254-265，269-283，287-300，306-31 6，318-336，338-352，374-392	K：17	162-174	12，229	gbs0113	Ribose abc transport albumen		F：4	267-274	11，228
gbs0123	Be similar to the argininosuccinic acid synthase		K：17	162-174	12，229	gbs0127	RpmV 50S ribosomal protein L28	30-42，45-54	F：11	25-37	13，230
gbs0144	Oligopeptides abc transport egg	10-30，53-59，86-95，116-130，132-147，169-	E：7	419-431	14，231	gbs0127	RpmV 50S ribosomal protein L28	30-42，45-54	F：11	25-37	13，230

The streptococcus agalactiae antigen protein	The function of inferring (passing through homology)	The immunogenicity amino acid * * that infers	Each ORF and the clone that selects of screening number	The position (amino acid) in the immunogenicity zone of differentiating	Seq. ID (DNA, protein)
The streptococcus agalactiae antigen protein	The function of inferring (passing through homology)	The immunogenicity amino acid * * that infers	Each ORF and the clone that selects of screening number		Seq. ID (DNA, protein)		In vain, substrate bonded	189，195-201，212-221，247-256，258-265，27 8-283，291-298，310-316，329-339，341-352， 360-367，388-396，398-411，416-432，443-45 2，460-466，506-512，515-521，542-548
gbs0183	Membrane protein, supposition	4-27，30-53，60-67，70-90，92-151，159-185， 189-195，198-210，215-239	F：9	173-189	15，232		In vain, substrate bonded
gbs0183	Membrane protein, supposition		F：9	173-189	15，232	gbs0184	Oligopeptides abc transport albumen, the oligopeptides bonded	4-26，41-54，71-78，116-127，140-149，151-1 58，161-175，190-196，201-208，220-226，240 -252，266-281，298-305，308-318，321-329，3 44-353，372-378，384-405，418-426，429-442 ，457-463，494-505，514-522	E：6	174-188	16，233
gbs0235	Glycinebetaine/carnitine/choline abc transport albumen	17-25，27-39，61-67，81-89，99-110，120-131 ，133-139，147-161，167-172，179-185，192-1 98，203-213，226-238，243-258，261-267，284 -290，296-307，311-328，340-352，356-371	G：8，H：15	239-256	17，234	gbs0184			E：6	174-188	16，233
gbs0235	Glycinebetaine/carnitine/choline abc transport albumen		G：8，H：15	239-256	17，234	gbs0255	Conservative hypothetical protein matter	8-30，40-49，67-80，114-123，126-142，152-1 62，188-194	E：2	57-70	18，235
gbs0260	Glycyl-tRNA synthetase (β-subunit)	4-23，28-34，36-47，50-61，76-81，89-94，96- 104，112-119，126-146，155-181，195-200，20 8-214，220-229，244-260，263-276，282-288， 292-300，317-323，336-351，353-359，363-37 5，382-399，415-432，444-455，458-471，476- 481，484-492，499-517，522-529，535-541，54 3-568，572-584，586-600，607-617，626-637， 656-675	F：3	282-297	19，236	gbs0255	Conservative hypothetical protein matter	8-30，40-49，67-80，114-123，126-142，152-1 62，188-194	E：2	57-70	18，235
gbs0260	Glycyl-tRNA synthetase (β-subunit)		F：3	282-297	19，236	gbs0268	Transketolase (tkt)	6-24，30-35，38-45，63-91，134-140，146-160 ，167-188，214-220，226-234，244-250，260-2 70，286-301，316-329，340-371，429-446，448 -459，474-481，485-491，512-526，537-544，5 50-565，573-583，596-613，621-630，652-658	E：7	87-97	20，237
gbs0286	NH3 dependency NAD+ synthetic enzyme	8-20，26-48，56-67，76-86，94-109，115-121， 123-129，143-160，178-186，191-198，201-20 8，221-236，238-244，260-268	F：12，G：7， H：8	237-247	21，238	gbs0268	Transketolase (tkt)		E：7	87-97	20，237
gbs0286	NH3 dependency NAD+ synthetic enzyme		F：12，G：7， H：8	237-247	21，238	gbs0288	Be similar to penicillin-binding protein 1A	4-40，42-57，73-87，98-117，126-135，150-15 6，166-174，196-217，231-236，248-258，276- 284，293-301，307-313，339-347，359-365，37 5-387，395-402，428-440，445-456，485-490， 497-505，535-541，547-555，610-625，648-65 6，665-671	D：5，K：3	448-528	22，239
gbs0343	Seryl-tRNA closes	10-18，39-45，51-61，80-96，98-106，110-115	I：3	322-338	23，240	gbs0288	Be similar to penicillin-binding protein 1A		D：5，K：3	448-528	22，239

The streptococcus agalactiae antigen protein	The function of inferring (passing through homology)	The immunogenicity amino acid * * that infers	Each ORF and the clone that selects of screening number	The position (amino acid) in the immunogenicity zone of differentiating	Seq. ID (DNA, protein)
The streptococcus agalactiae antigen protein	The function of inferring (passing through homology)	The immunogenicity amino acid * * that infers	Each ORF and the clone that selects of screening number		Seq. ID (DNA, protein)		Become enzyme	，158-172，174-183，191-200，220-237，249-2 55，274-289，308-324，331-341，372-381，384 -397，405-414
gbs0411	Hypothetical protein matter	30-36，38-56，85-108，134-147，149-160，163 -183，188-201，206-211，219-238，247-254	I：11	5-13	24，241		Become enzyme
gbs0411	Hypothetical protein matter		I：11	5-13	24，241	gbs0428	Be similar to fibrinogen binding protein, the peptidoglycan of supposition connects albumen (LPXTG group)	11-40，98-103，110-115，133-145，151-159，1 72-179，192-201，204-212，222-228，235-245 ，258-268，283-296，298-309，322-329，342-3 51，354-362，372-378，385-393，407-418，495 -516	A：7，B：2， C：31	1-148	25，242
gbs0437	G-6-P isomerase (pgi)	5-19，21-36，73-94，112-119，122-137，139-1 45，152-167，184-190，198-204，208-224，249 -265，267-281，299-304，309-317，326-333，3 56-364，368-374，381-389，391-414，419-425 ，430-435	I：26	113-140	26，243	gbs0428			A：7，B：2， C：31	1-148	25，242
gbs0437	G-6-P isomerase (pgi)		I：26	113-140	26，243	gbs0460	Decarboxylase	45-54，59-67，78-91	I：7，K：11	15-23	27，244
gbs0465	Oxydo-reductase	11-22，33-47，52-80，88-112，124-129	F：4	6-25	28，245	gbs0460	Decarboxylase	45-54，59-67，78-91	I：7，K：11	15-23	27，244
gbs0465	Oxydo-reductase	11-22，33-47，52-80，88-112，124-129	F：4	6-25	28，245	gbs0470	Be similar to alpha protein, the peptidoglycan of supposition connects albumen (LPXTG group)	26-41，51-63，80-89，93-115，150-163，187-1 93，220-237，240-249，286-294，296-306，316 -329，345-353，361-370，407-425，428-437，4 74-482，484-494，504-517，533-541，549-558 ，595-613，616-625，660-668，673-685，711-7 26，736-744，749-761，787-802，812-820，825 -837，863-878，888-896，901-913，939-954，9 64-972，977-989，1003-1008，1016-1022，102 8-1034，1041-1053，1059-1074，1101-1122	B：4，C：2， D：8	420-511， 581-704	29，246
gbs0489	Acyltransferase, GNAT family	18-25，27-55，71-83，89-95，102-113，120-14 6，150-156，174-185	E：32	159-175	30，247	gbs0470			B：4，C：2， D：8	420-511， 581-704	29，246
gbs0489	Acyltransferase, GNAT family	18-25，27-55，71-83，89-95，102-113，120-14 6，150-156，174-185	E：32	159-175	30，247	gbs0492	The gbs0492 Valyl-tRNA synthetase	24-30，38-56，63-68，87-93，136-142，153-16 4，183-199，213-219，226-234，244-261，269- 278，283-289，291-297，320-328，330-336，34 0-346，348-356，358-366，382-387，401-408， 414-419，449-455，468-491，504-512，531-53 7，554-560，597-608，621-627，632-643，650- 662，667-692，703-716，724-737，743-758，78 3-794，800-818，846-856	A：3	806-884	31，248
gbs0538	Amino acid abc transporter protein (ATP is conjugated protein)	4-14，21-39，86-92，99-107，121-131，136-14 4，147-154，158-166，176-185，193-199，207- 222，224-230	G：1	117-136	32，249	gbs0492	The gbs0492 Valyl-tRNA synthetase		A：3	806-884	31，248
gbs0538			G：1	117-136	32，249	gbs0539	Be similar to mannose-phosphate mutase	65-76，85-97，103-109，115-121，125-146，16 3-169，196-205，212-219，228-237，241-247，	K：4	266-284	33，250

The streptococcus agalactiae antigen protein	The function of inferring (passing through homology)	The immunogenicity amino acid * * that infers	Each ORF and the clone that selects of screening number	The position (amino acid) in the immunogenicity zone of differentiating	Seq. ID (DNA, protein)
The streptococcus agalactiae antigen protein	The function of inferring (passing through homology)	The immunogenicity amino acid * * that infers	Each ORF and the clone that selects of screening number		Seq. ID (DNA, protein)			254-262，269-288，294-303，305-313，328-36 7，395-401，405-412，418-429，437-447，481- 488，506-513，519-524，530-541，546-557
gbs0555	Beta-lactam resistance factor (fibA)	5-14，37-42，49-71，78-92，97-112，127-136， 147-154，156-163，186-198，216-225，233-24 3，248-253，295-307，323-332，359-366，368- 374，380-398	E：3	194-223	34，251
gbs0555	Beta-lactam resistance factor (fibA)		E：3	194-223	34，251	gbs0579	Pepx	4-11，33-39，45-72，100-113，119-129，136-1 44，169-175，177-185，200-208，210-219，262 -276，278-297，320-326，336-344，347-362，3 81-394，443-453	I：4	438-454	35，252
gbs0580	Zinc ABC shifts son, and zinc is in conjunction with adhesion, lipoprotein	4-29，31-52，55-61，95-110，138-158，162-17 1，179-187，202-229，239-248，251-256，262- 267，269-285，304-310，351-360，362-368，38 1-388，415-428，435-440，448-458	I：11	161-178	36，253	gbs0579	Pepx		I：4	438-454	35，252
gbs0580			I：11	161-178	36，253	gbs0628	Cell walls surface anchoring family protein (IPxTG)	4-17，19-28，32-43，47-59，89-110，112-126， 128-134，140-148，152-161，169-184，191-20 4，230-235，255-264，328-338，341-347，401- 409，413-419，433-441，449-458，463-468，47 6-482，486-492，500-506，529-545	I：9，H：1	305-381	37，254
gbs0632	Cell walls surface anchoring family protein, (the FPKTG group) of supposition	10-29，38-45，53-61，134-145，152-160，163- 170，202-208，219-229，248-258，266-275，28 2-288，315-320，328-334，377-385，392-402， 418-424，447-453，460-471，479-487，491-49 7，500-507，531-537，581-594，615-623，629- 635，644-652，659-666，668-678，710-717，71 9-728，736-741，747-760，766-773，784-789， 794-800，805-817，855-861，866-887	H：3	698-715	38，255	gbs0628	Cell walls surface anchoring family protein (IPxTG)		I：9，H：1	305-381	37，254
gbs0632			H：3	698-715	38，255	gbs0634	The surface protein of inferring	16-26，29-37，44-58，62-68，74-80，88-95，97 -120，125-144，165-196	H：1	58-72	39，256
gbs0667	Regulate protein, supposition, block	14-21，23-46，49-60，63-74，78-92，96-103，1 17-129，134-161，169-211，217-231，239-248 ，252-281，292-299，313-343	I：2	243-257	40，257	gbs0634	The surface protein of inferring	16-26，29-37，44-58，62-68，74-80，88-95，97 -120，125-144，165-196	H：1	58-72	39，256
gbs0667	Regulate protein, supposition, block		I：2	243-257	40，257	gbs0672	Transcriptional regulatory (GntR family)	11-27，46-52，67-72，76-84，91-112，116-153 ，160-175，187-196，202-211，213-220	G：11	43-76	41，258
gbs0687	Hypothetical protein matter	5-29，37-56，78-86，108-118，152-161	I：4	120-130	42，259	gbs0672	Transcriptional regulatory (GntR family)		G：11	43-76	41，258
gbs0687	Hypothetical protein matter	5-29，37-56，78-86，108-118，152-161	I：4	120-130	42，259	gbs0785	Be similar to penicillin-binding protein 2B	8-14，19-41，52-66，75-82，87-92，106-121，1 27-133，136-143，158-175，180-187，196-204 ，221-228，239-245，259-265，291-306，318-3 23，328-340，352-358，361-368，375-381，391	K：3	184-196	43，260

The streptococcus agalactiae antigen protein	The function of inferring (passing through homology)	The immunogenicity amino acid * * that infers	Each ORF and the clone that selects of screening number	The position (amino acid) in the immunogenicity zone of differentiating	Seq. ID (DNA, protein)
The streptococcus agalactiae antigen protein	The function of inferring (passing through homology)	The immunogenicity amino acid * * that infers	Each ORF and the clone that selects of screening number		Seq. ID (DNA, protein)			-399，411-418，431-442，446-455，484-496，4 98-510，527-533，541-549，558-565，575-585 ，587-594，644-655，661-668，671-677
gbs0811	Beta-glucosidase	4-22，29-38，55-62，75-81，102-107，110-134 ，143-150，161-167，172-179，191-215，223-2 33，241-247，251-264，266-272，288-309，340 -352，354-366，394-402，414-438	L：13	198-218	44，261
gbs0811	Beta-glucosidase		L：13	198-218	44，261	gbs0828	Hypothetical protein matter	24-44，49-70，80-91，105-118，128-136，140- 154	I：3	77-92	45，262
gbs0851	Hypothetical protein matter	5-22，31-36，41-47，67-74，83-90，105-122，1 35-143，160-167	E：2	118-129	46，263	gbs0828	Hypothetical protein matter	24-44，49-70，80-91，105-118，128-136，140- 154	I：3	77-92	45，262
gbs0851	Hypothetical protein matter	5-22，31-36，41-47，67-74，83-90，105-122，1 35-143，160-167	E：2	118-129	46，263	gbs0865	Hypothetical protein matter	4-25，33-73，81-93，96-106，114-120，122-12 8，130-172，179-208，210-241，251-283，296- 301	K：4	92-100	47，264
gbs0890	Exonuclease RexB (rexB)	14-24，29-38，43-50，52-72，86-97，101-107， 110-125，127-141，145-157，168-175，177-18 4，186-195，205-226，238-250，255-261，284- 290，293-304，307-314，316-323，325-356，36 3-371，383-390，405-415，423-432，442-454， 466-485，502-511，519-527，535-556，558-56 5，569-574，612-634，641-655，672-686，698- 709，715-722，724-732，743-753，760-769，78 3-792，818-825，830-839，842-849，884-896， 905-918，926-940，957-969，979-1007，1015- 1021，1049-1057	E：5	336-349	48，265	gbs0865	Hypothetical protein matter		K：4	92-100	47，264
gbs0890	Exonuclease RexB (rexB)		E：5	336-349	48，265	gbs0896	Be similar to the 3-acetoin dehydrogenase	6-16，26-31，33-39，62-73，75-85，87-100，11 3-123，127-152，157-164，168-181，191-198， 208-214，219-226，233-254，259-266，286-32 9	K：2	181-195	49，266
gbs0898	The 3-acetoin dehydrogenase, thymus pyrimidine PPi is dependent	4-13，32-39，53-76，99-108，110-116，124-13 5，137-146，149-157，162-174，182-190，207- 231，242-253，255-264，274-283，291-323，33 4-345，351-360，375-388，418-425，456-474， 486-492，508-517，520-536，547-560，562-57 7	E：13，F：2， I：2，J：2	31-45， 419-443	50，267	gbs0896	Be similar to the 3-acetoin dehydrogenase		K：2	181-195	49，266
gbs0898			E：13，F：2， I：2，J：2	31-45， 419-443	50，267	gbs0904	Phosphoglucomutase/mannose-phosphate mutase family protein	15-26，30-37，42-49，58-90，93-99，128-134， 147-154，174-179，190-197，199-205，221-23 0，262-274，277-287，300-314，327-333，343- 351，359-377，388-396，408-413，416-425，43 1-446	I：3	246-256	51，268
gbs0918	Be similar to three a little	5-26，34-42，47-54，61-67，71-104，107-115，	B：5，C：11，	14-138，	52，269	gbs0904	Phosphoglucomutase/mannose-phosphate mutase family protein		I：3	246-256	51，268

The streptococcus agalactiae antigen protein	The function of inferring (passing through homology)	The immunogenicity amino acid * * that infers	Each ORF and the clone that selects of screening number	The position (amino acid) in the immunogenicity zone of differentiating	Seq. ID (DNA, protein)
The streptococcus agalactiae antigen protein	The function of inferring (passing through homology)	The immunogenicity amino acid * * that infers	Each ORF and the clone that selects of screening number		Seq. ID (DNA, protein)		Histidine protein matter, the lipoprotein of supposition	131-138，144-153，157-189，196-202，204-21 0，228-245，288-309，316-329，332-341，379- 386，393-399，404-412，414-421，457-468，48 3-489，500-506，508-517，523-534，543-557， 565-580，587-605，609-617，619-627，631-63 6，640-646，662-668，675-682，705-710，716- 723，727-732，750-758，784-789，795-809，86 9-874	D：36，E：3， K：3	166-286， 372-503， 674-696， 754-859
gbs0931	Pyruvate kinase	5-17，32-38，40-47，80-89，113-119，125-137 ，140-154，157-163，170-177，185-199，213-2 25，228-236，242-248，277-290，292-305，323 -333，347-353，364-370，385-394，399-406，4 23-433，441-451，462-474，477-487	F：78	116-124	53，270		Histidine protein matter, the lipoprotein of supposition		D：36，E：3， K：3	166-286， 372-503， 674-696， 754-859
gbs0931	Pyruvate kinase		F：78	116-124	53，270	gbs0947	Similar sub-L-lactose desaturase	7-16，18-30，32-49，53-61，63-85，95-101，10 5-115，119-134，143-150，159-178，185-202， 212-229，236-250，254-265，268-294	K：28	63-72	54，271
gbs0948	Dna helicase, A subunit (gyrA)	4-12，19-47，73-81，97-103，153-169，188-19 8，207-213，217-223，236-242，255-265，270- 278，298-305，309-317，335-347，354-363，37 3-394，419-424，442-465，486-492，500-507， 542-549，551-558，560-572，580-589，607-61 4，617-623，647-653，666-676，694-704，706- 714，748-754，765-772，786-792，795-806	I：4	358-370	55，272	gbs0947	Similar sub-L-lactose desaturase		K：28	63-72	54，271
gbs0948	Dna helicase, A subunit (gyrA)		I：4	358-370	55，272	gbs0969	Be similar to unknown plasmid protein	18-28，30-38，40-46，49-55，69-78，82-98，10 4-134，147-153，180-190，196-202，218-236， 244-261，266-273，275-286，290-295，301-31 4，378-387，390-395，427-434	E：3	290-305	56，273
gbs0971	Be similar to the plasmid replication albumen of supposition	4-13，20-31，39-51，54-61，69-84，87-105，11 7-124	K：17	108-125	57，274	gbs0969	Be similar to unknown plasmid protein		E：3	290-305	56，273
gbs0971		4-13，20-31，39-51，54-61，69-84，87-105，11 7-124	K：17	108-125	57，274	gbs0972	Hypothetical protein matter	24-34，43-54，56-66，68-79	E：3	50-69	58，275
gbs0983	Be similar to plasmid protein	5-43，71-77，102-131，141-148，150-156，159 -186，191-207，209-234，255-268，280-286，2 93-299，317-323，350-357，363-372，391-397 ，406-418，428-435，455-465，484-497，499-5 05，525-531，575-582，593-607，621-633，638 -649，655-673，684-698，711-725，736-741，7 43-752，759-769，781-793，813-831，843-853 ，894-905，908-916，929-946，953-963，970-9 78，1001-1007，1011-1033	D：11，E：2， F：2，J：10， K：10， L：46，M：3	165-178， 818-974	59，276	gbs0972	Hypothetical protein matter	24-34，43-54，56-66，68-79	E：3	50-69	58，275
gbs0983	Be similar to plasmid protein		D：11，E：2， F：2，J：10， K：10， L：46，M：3	165-178， 818-974	59，276	gbs0986	Surface antigen protein matter, the binding peptide of supposition is poly-	16-44，63-86，98-108，185-191，222-237，261 -274，282-294，335-345，349-362，374-384，4	B：3，C：12， D：2，E：3，	77-90， 144-212，	60，277

The streptococcus agalactiae antigen protein	The function of inferring (passing through homology)	The immunogenicity amino acid * * that infers	Each ORF and the clone that selects of screening number	The position (amino acid) in the immunogenicity zone of differentiating	Seq. ID (DNA, protein)
The streptococcus agalactiae antigen protein	The function of inferring (passing through homology)	The immunogenicity amino acid * * that infers	Each ORF and the clone that selects of screening number		Seq. ID (DNA, protein)		Sugar	09-420，424-430，440-447，453-460，465-473 ，475-504，522-534，538-551，554-560，567-5 82，598-607，611-619，627-640，643-653，655 -661，669-680，684-690，701-707，715-731，7 44-750，756-763，768-804，829-837，845-853 ，855-879，884-890，910-928	F：20，H：3， I：3，J：5， M：2	279-355， 434-536， 782-810， 875-902
gbs0988	Be similar to the plasmid surface and get rid of albumen, the binding peptide glycan protein (LPXTG group) of supposition	4-22，29-41，45-51，53-66，70-77，86-95，98- 104，106-124，129-135，142-151，153-161，16 9-176，228-251，284-299，331-337，339-370， 380-387，393-398，406-411，423-433，440-45 2，461-469，488-498，501-516，523-530，532- 559，562-567，570-602，612-628，630-645，64 9-659，666-672，677-696，714-723，727-747	J：2	212-227	61，278		Sugar		F：20，H：3， I：3，J：5， M：2	279-355， 434-536， 782-810， 875-902
gbs0988			J：2	212-227	61，278	gbs0991	ATP dependency C1p proteolytic enzyme, ATP binding subunit C1pA	4-9，17-31，35-41，56-61，66-75，81-87，90-1 24，133-138，149-163，173-192，213-219，221 -262，265-275，277-282，292-298，301-307，3 33-346，353-363，371-378，419-430，435-448 ，456-469，551-570，583-599，603-612	F：15	275-291	62，279
gbs0993	Be similar to plasmid protein	28-34，53-58，72-81，100-128，145-154，159- 168，172-189，217-225，227-249，256-263，29 9-309，322-330，361-379，381-388，392-401， 404-417，425-436，440-446，451-464，469-48 7，502-511，543-551，559-564，595-601，606- 612，615-626，633-642，644-650，664-670，67 4-684，692-701，715-723，726-734，749-756， 763-771，781-787，810-843，860-869，882-88 9，907-917，931-936，941-948，951-958，964- 971，976-993，1039-1049，1051-1065，1092-1 121，1126-1132，1145-1151，1158-1173，1181 -1192，1194-1208，1218-1223，1229-1243，12 49-1254，1265-1279，1287-1297，1303-1320， 1334-1341，1343-1358，1372-1382，1406-141 7，1419-1425，1428-1434，1441-1448，1460-1 473，1494-1504，1509-1514，1529-1550	B：2，F：2， J：4，K：2， M：7	654-669， 1400-1483	63，280	gbs0991			F：15	275-291	62，279
gbs0993	Be similar to plasmid protein		B：2，F：2， J：4，K：2， M：7	654-669， 1400-1483	63，280	gbs0995	Hypothetical protein matter	10-16，20-25，58-65，97-109，118-132，134-1 46，148-155，186-195，226-233，244-262，275 -284，295-310，317-322，330-339，345-351，3 66-375，392-403，408-415，423-430，435-444 ，446-457，467-479，486-499，503-510，525-5 37，540-585，602-612，614-623，625-634，639 -645，650-669，700-707，717-724，727-739	H：3，I：39， J：3，M：3	205-230， 733-754	64，281

The streptococcus agalactiae antigen protein	The function of inferring (passing through homology)	The immunogenicity amino acid * * that infers	Each ORF and the clone that selects of screening number	The position (amino acid) in the immunogenicity zone of differentiating	Seq. ID (DNA, protein)
The streptococcus agalactiae antigen protein	The function of inferring (passing through homology)	The immunogenicity amino acid * * that infers	Each ORF and the clone that selects of screening number		Seq. ID (DNA, protein)	gbs0997	Hypothetical protein matter	5-22，37-43，72-81，105-113，128-133，148-1 60，188-194，204-230，238-245，251-257	D：2，F：52	194-213	65，282
gbs0998	Hypothetical protein matter	16-21，35-41，56-72，74-92，103-109	I：2	62-68	66，283	gbs0997	Hypothetical protein matter		D：2，F：52	194-213	65，282
gbs0998	Hypothetical protein matter	16-21，35-41，56-72，74-92，103-109	I：2	62-68	66，283	gbs1001	Hypothetical protein matter	4-15，17-82，90-104，107-159，163-170，188- 221，234-245，252-265	G：8	220-235	67，284
gbs1015	Hypothetical protein matter	16-22，36-46，61-75，92-107，113-121，139-1 45，148-160	K：17	30-42	68，285	gbs1001	Hypothetical protein matter	4-15，17-82，90-104，107-159，163-170，188- 221，234-245，252-265	G：8	220-235	67，284
gbs1015	Hypothetical protein matter	16-22，36-46，61-75，92-107，113-121，139-1 45，148-160	K：17	30-42	68，285	gbs1035	Conservative hypothetical protein matter	4-12，20-26，43-49，55-62，66-78，121-127，1 35-141，146-161，164-170，178-189，196-205 ，233-238，269-279，288-318，325-332，381-3 86，400-407	E：3	328-346	69，286
gbs1041	Hypothetical protein matter	5-12，31-49，57-63，69-79，89-97，99-114，11 6-127，134-142，147-154，160-173，185-193， 199-204，211-222，229-236，243-249，256-27 4	L：2	58-68	70，287	gbs1035	Conservative hypothetical protein matter		E：3	328-346	69，286
gbs1041	Hypothetical protein matter		L：2	58-68	70，287	gbs1066	Hypothetical protein matter	10-20，28-34，39-53，68-79，84-90，99-106	K：2	73-79	71，288
gbs1087	FbsA	14-37，45-50，61-66，77-82，93-98，109-114， 125-130，141-146，157-162，173-178，189-19 4，205-210，221-226，237-242，253-258，269- 274，285-290，301-306，316-332，349-359，37 1-378，385-406	A：7，B：2， C：4， E：277， G：523， J：25	34-307， 312-385	72，289	gbs1066	Hypothetical protein matter	10-20，28-34，39-53，68-79，84-90，99-106	K：2	73-79	71，288
gbs1087	FbsA		A：7，B：2， C：4， E：277， G：523， J：25	34-307， 312-385	72，289	gbs1103	Abc transporter protein (ATP is conjugated protein)	4-10，17-38，50-85，93-99，109-116，128-185 ，189-197，199-210，223-256，263-287，289-3 12，327-337，371-386，389-394，406-419，424 -432，438-450，458-463，475-502，507-513，5 19-526，535-542，550-567	I：5	361-376	73，290
gbs1116	Xanthine permease (pbuX)	10-39，42-93，100-144，155-176，178-224，23 0-244，246-255，273-282，292-301，308-325， 332-351，356-361，368-379，386-393，400-42 1	I：48	138-155	74，291	gbs1103	Abc transporter protein (ATP is conjugated protein)		I：5	361-376	73，290
gbs1116	Xanthine permease (pbuX)		I：48	138-155	74，291	gbs1126	Be similar to plasmid agnoprotein matter	5-11，17-34，40-45，50-55，72-80，101-123，1 45-151，164-172，182-187，189-195，208-218 ，220-241，243-252，255-270，325-331，365-3 71，391-398，402-418，422-428，430-435，443 -452，463-469，476-484，486-494，503-509，5 29-553，560-565，570-590，608-614，619-627 ，654-661，744-750，772-780，784-790，806-8 16，836-853，876-885，912-918，926-933，961 -975，980-987，996-1006，1016-1028，1043-1 053，1057-1062	E：2，K：3	994-1003， 1033-1056	75，292
gbs1143	The dextran of inferring connects	17-45，64-71，73-81，99-109，186-192，223-2	C：3，D：2，	274-350，	76，293	gbs1126	Be similar to plasmid agnoprotein matter		E：2，K：3	994-1003， 1033-1056	75，292

The streptococcus agalactiae antigen protein	The function of inferring (passing through homology)	The immunogenicity amino acid * * that infers	Each ORF and the clone that selects of screening number	The position of differentiating of exempting from disease originality zone (amino acid)	Seq. ID (DNA, protein)
The streptococcus agalactiae antigen protein	The function of inferring (passing through homology)	The immunogenicity amino acid * * that infers	Each ORF and the clone that selects of screening number		Seq. ID (DNA, protein)		Connect albumen (LPXTG)	38，262-275，283-295，336-346，350-363，375 -385，410-421，425-431，441-448，454-463，4 68-474，476-512，523-537，539-552，568-583 ，599-608，612-620，628-641，644-654，656-6 62，670-681，685-695，702-708，716-723，725 -735，757-764，769-798，800-806，808-816，8 26-840，846-854，856-862，874-881，885-902 ，907-928	F：15，J：3	443-513
gbs1145	Protein S ec10 is got rid of on the surface	4-22，29-41，45-51，53-61，70-76，85-92，99- 104，111-122，134-140，142-154，163-174，22 4-232，255-265，273-279，283-297，330-335， 337-348，356-367，373-385，391-396，421-43 1，442-455，475-485，493-505，526-538，544- 561，587-599，605-620，622-651，662-670，67 5-681，687-692，697-712，714-735	C：2	252-262	77，294		Connect albumen (LPXTG)		F：15，J：3	443-513
gbs1145	Protein S ec10 is got rid of on the surface		C：2	252-262	77，294	gbs1158	Be similar to oxydo-reductase	4-12，15-35，40-46，50-59，67-94，110-128，1 43-169，182-188，207-215，218-228，238-250	K：2	74-90	78，295
gbs1165	Disulfurase (iscS-1)	9-18，42-58，78-85，88-95，97-106，115-122， 128-134，140-145，154-181，186-202，204-22 3，261-267，269-278，284-293，300-336，358- 368	F：5	12-29	79，296	gbs1158	Be similar to oxydo-reductase		K：2	74-90	78，295
gbs1165	Disulfurase (iscS-1)		F：5	12-29	79，296	gbs1195	Staphylokinase and streptokinase	7-34，46-53，62-72，82-88，100-105，111-117 ，132-137，144-160，166-180，183-189，209-2 21，231-236，246-253，268-282，286-293，323 -336，364-372，378-392，422-433	B：3，C：2， D：2，G：3， H：8	388-405	80，297
gbs1209	ATP dependent DNA helicase PcrA	21-27，34-50，72-77，80-95，164-177，192-19 8，202-220，226-236，239-247，270-279，285- 292，315-320，327-334，348-355，364-371，38 8-397，453-476，488-497，534-545，556-576， 582-588，601-607，609-616，642-662，674-68 1，687-697，709-715，721-727，741-755	C：3，I：5	621-739	81，298	gbs1195	Staphylokinase and streptokinase		B：3，C：2， D：2，G：3， H：8	388-405	80，297
gbs1209	ATP dependent DNA helicase PcrA		C：3，I：5	621-739	81，298	gbs1214	Conservative hypothetical protein matter	4-14，16-77，79-109	B：2	25-99	82，299
gbs1242	CpsG, β-1,4-galactotransferase	4-9，17-23，30-37，44-55，65-72，77-93，102- 121，123-132，146-153	L：24	17-29	83，300	gbs1214	Conservative hypothetical protein matter	4-14，16-77，79-109	B：2	25-99	82，299
gbs1242	CpsG, β-1,4-galactotransferase	4-9，17-23，30-37，44-55，65-72，77-93，102- 121，123-132，146-153	L：24	17-29	83，300	gbs1260	Abc transport albumen, ATP is conjugated protein	4-18，25-41，52-60，83-92，104-112，117-123 ，149-155，159-167，170-192，201-210，220-2 27，245-250	I：17	124-137	84，301
gbs1270	The gbs1270 hyaluronate lyase	8-25，50-55，89-95，138-143，148-153，159-1 69，173-179，223-238，262-268，288-295，297	C：19，D：5， L：19	1-128， 252-341，	85，302	gbs1260	Abc transport albumen, ATP is conjugated protein		I：17	124-137	84，301

The streptococcus agalactiae antigen protein	The function of inferring (passing through homology)	The immunogenicity amino acid * * that infers	Each ORF and the clone that selects of screening number	The position (amino acid) in the immunogenicity zone of differentiating	Seq. ID (DNA, protein)
The streptococcus agalactiae antigen protein	The function of inferring (passing through homology)	The immunogenicity amino acid * * that infers	Each ORF and the clone that selects of screening number		Seq. ID (DNA, protein)			-308，325-335，403-409，411-417，432-446，4 63-475，492-501，524-530，542-548，561-574 ，576-593，604-609，612-622，637-654，665-6 72，678-685，720-725，731-739，762-767，777 -783，820-838，851-865，901-908，913-920，9 58-970，1000-1006，1009-1015，1020-1026，1 043-1052，1055-1061		771-793， 1043-1058
gbs1305	Hypothetical protein matter	16-26，33-46	I：2	64-76	86，303					771-793， 1043-1058
gbs1305	Hypothetical protein matter	16-26，33-46	I：2	64-76	86，303	gbs1306	Ln is conjugated protein	4-27，69-77，79-101，117-123，126-142，155- 161，171-186，200-206，213-231，233-244，26 7-273，313-329，335-344，347-370，374-379， 399-408，422-443，445-453，461-468，476-48 2，518-534，544-553，556-567，578-595，601- 620，626-636，646-658，666-681，715-721，76 2-768，778-785，789-803，809-819	A：6，B：7， C：17， D：72，E：8， F：91，G：2， H：4，I：26， J：3，K：14	22-108， 153-318， 391-527， 638-757	87，304
gbs1307	Lmb, ln mating surface albumen	6-21，32-43，62-92，104-123，135-141，145-1 52，199-216，218-226，237-247，260-269，274 -283，297-303	A：2，D：3	1-72， 127-211	88，305	gbs1306	Ln is conjugated protein		A：6，B：7， C：17， D：72，E：8， F：91，G：2， H：4，I：26， J：3，K：14	22-108， 153-318， 391-527， 638-757	87，304
gbs1307	Lmb, ln mating surface albumen		A：2，D：3	1-72， 127-211	88，305	gbs1308	The C5a peptase, true frameshit	6-26，50-56，83-89，108-114，123-131，172-1 81，194-200，221-238，241-247，251-259，263 -271，284-292，304-319，321-335，353-358，3 84-391，408-417，424-430，442-448，459-466 ，487-500，514-528，541-556，572-578，595-6 01，605-613，620-631，635-648，660-670，673 -679，686-693，702-708，716-725，730-735，7 49-755，770-777，805-811，831-837，843-851 ，854-860，863-869，895-901，904-914，922-9 29，933-938，947-952，956-963，1000-1005，1 008-1014，1021-1030，1097-1103，1120-1130 ，1132-1140	B：4，C：15， D：70， E：18， F：26，G：5， H：4，J：2， K：40	1-213， 269-592， 992-1120	89，306
gbs1309	Hypothetical protein matter	9-16，33-39，47-59，65-79，81-95，103-108，1 15-123，138-148，163-171，176-185，191-196 ，205-211，213-221，224-256，261-276，294-3 02，357-363，384-390	E：2，F：4， H：2，J：2	95-111， 161-189	90，307	gbs1308	The C5a peptase, true frameshit		B：4，C：15， D：70， E：18， F：26，G：5， H：4，J：2， K：40	1-213， 269-592， 992-1120	89，306
gbs1309	Hypothetical protein matter		E：2，F：4， H：2，J：2	95-111， 161-189	90，307	gbs1311	Translocase, the C-terminal part	21-27，35-45，70-76，92-105，129-143，145-1 55，161-166，170-191，204-211，214-231，234 -246，249-255，259-275	F：3	1-18	91，308
gbs1321	Hypothetical protein matter	21-35，45-53，56-64，69-97	F：7	1-16	92，309	gbs1311	Translocase, the C-terminal part		F：3	1-18	91，308
gbs1321	Hypothetical protein matter	21-35，45-53，56-64，69-97	F：7	1-16	92，309	gbs1352	Helicase and the methylase inferred	25-33，41-47，61-68，86-101，106-114，116-1 29，134-142，144-156，163-176，181-190，228 -251，255-261，276-292，295-305，334-357，3	E：3，H：2， M：4	748-847， 1381-1391	93，310

The streptococcus agalactiae antigen protein	The function of inferring (passing through homology)	The immunogenicity amino acid * * that infers	Each ORF and the clone that selects of screening number	The position (amino acid) in the immunogenicity zone of differentiating	Seq. ID (DNA, protein)
The streptococcus agalactiae antigen protein	The function of inferring (passing through homology)	The immunogenicity amino acid * * that infers	Each ORF and the clone that selects of screening number		Seq. ID (DNA, protein)			68-380，395-410，424-429，454-460，469-482 ，510-516，518-527，531-546，558-570，579-6 06，628-636，638-645，651-656，668-674，691 -698，717-734，742-754，765-770，792-797，8 27-835，847-859，874-881，903-909，926-933 ，942-961，964-977，989-1004，1010-1028，10 31-1047，1057-1075，1081-1095，1108-1117， 1138-1144，1182-1189，1193-1206，1220-122 9，1239-1246，1257-1267，1271-1279，1284-1 301，1312-1320，1329-1335，1341-1347，1358 -1371，1399-1404，1417-1426，1458-1463，14 68-1476，1478-1485，1493-1506，1535-1541， 1559-1574，1583-1590，1595-1601，1603-161 1，1622-1628，1634-1644，1671-1685，1689-1 696，1715-1720，1734-1746，1766-1775，1801 -1806，1838-1844，1858-1871，1910-1917，19 48-1955，1960-1974，2000-2015，2019-2036， 2041-2063
gbs1356	The poly-enzyme of the peptide of inferring connects albumen (LPXTG group)-agglutinin receptor	5-12，18-24，27-53，56-63，96-113，119-124， 131-136，157-163，203-209，215-223，233-24 6，264-273，311-316，380-389，393-399，425- 433，445-450，457-462，464-470，475-482，50 7-513，527-535，542-548，550-565，591-602， 607-613，627-642，644-664，673-712，714-73 2，739-764，769-782，812-818，826-838，848- 854，860-871，892-906，930-938，940-954，95 7-973，990-998，1002-1021，1024-1033，1037 -1042，1050-1060，1077-1083，1085-1092，11 00-1129，1144-1161，1169-1175，1178-1189， 1192-1198，1201-1207，1211-1221，1229-123 9，1250-1270，1278-1292，1294-1300，1314-1 335，1344-1352，1360-1374，1394-1405，1407 -1414，1416-1424，1432-1452，1456-1462，14 74-1497，1500-1510，1516-1522，1534-1542， 1550-1559，1584-1603，1608-1627	C：5，D：62， I：22	187-273， 306-441	94，311
gbs1356			C：5，D：62， I：22	187-273， 306-441	94，311	gbs1376	Be similar to ATP dependency C1p proteolytic enzyme (ATP binding subunit), C1pL	70-80，90-97，118-125，128-140，142-148，15 4-162，189-202，214-222，224-232，254-260， 275-313，317-332，355-360，392-398，425-43 2，448-456，464-470，476-482，491-505，521- 528，533-546，560-567，592-597，605-614，61 8-626，637-644，646-653，660-666，677-691	K：4	207-227	95，312
gbs1377	Be similar to homotype half Guang	5-19，26-34，37-55，57-66，69-83，86-102，11	M：2	256-266	96，313	gbs1376			K：4	207-227	95，312

The streptococcus agalactiae antigen protein	The function of inferring (passing through homology)	The immunogenicity amino acid * * that infers	Each ORF and the clone that selects of screening number	The position (amino acid) in the immunogenicity zone of differentiating	Seq. ID (DNA, protein)
The streptococcus agalactiae antigen protein	The function of inferring (passing through homology)	The immunogenicity amino acid * * that infers	Each ORF and the clone that selects of screening number		Seq. ID (DNA, protein)		Propylhomoserin-S-methyltransgerase	5-134，138-143，154-172，178-195，209-246， 251-257，290-302，306-311
gbs1386	The hydroxy-3-methyl glutaryl coenzyme A synthase	10-20，22-28，35-57，72-79，87-103，108-128 ，130-144，158-171，190-198，225-242，274-2 91，301-315，317-324，374-385	G：2	353-365	97，314		Propylhomoserin-S-methyltransgerase
gbs1386	The hydroxy-3-methyl glutaryl coenzyme A synthase		G：2	353-365	97，314	gbs1390	Hypothetical protein matter	4-9，17-30，34-54，59-66，73-94，118-130，13 5-150，158-171，189-198，219-239，269-275， 283-301	E：3，K：4	89-106， 176-193	98，315
gbs1391	Hypothetical protein matter	14-20，22-74，77-86，89-99，104-109，126-13 5，154-165，181-195，197-212，216-224，264- 275	E：3	107-118	99，316	gbs1390	Hypothetical protein matter		E：3，K：4	89-106， 176-193	98，315
gbs1391	Hypothetical protein matter		E：3	107-118	99，316	gbs1403	Similar in appearance to 5 '-phosphonuclease, the binding peptide of supposition gathers zymoprotein (LPXTN)	4-18，21-38，63-72，101-109，156-162，165-1 79，183-192，195-210，212-218，230-239，241 -256，278-290，299-311，313-322，332-341，3 48-366，386-401，420-426，435-450，455-460 ，468-479，491-498，510-518，532-538，545-5 52，557-563，567-573，586-595，599-609，620 -626，628-636，652-657，665-681	A：3，C：12， D：4，J：2	1-198	100， 317
gbs1408	Be similar to abc transport albumen (ATP is conjugated protein)	4-10，16-38，51-68，73-79，94-115，120-125， 132-178，201-208，216-223，238-266，269-29 5，297-304，337-342，347-356，374-401，403- 422，440-447，478-504，510-516，519-530，53 7-544	D：2，K：4	191-206	101， 318	gbs1403			A：3，C：12， D：4，J：2	1-198	100， 317
gbs1408			D：2，K：4	191-206	101， 318	gbs1420	Be similar to cell wall protein, the peptidoglycan of supposition connects albumen (LPXTG group)	12-40，42-48，66-71，77-86，95-102，113-120 ，129-137，141-148，155-174，208-214，218-2 25，234-240，256-267，275-283，300-306，313 -321，343-350，359-367，370-383，398-405，4 32-439，443-461，492-508，516-526，528-535	C：3，D：4	370-478	102， 319
gbs1429	Hypothetical protein matter	6-14，20-37，56-62，90-95，97-113，118-125， 140-145，161-170，183-202，237-244，275-28 4，286-305，309-316，333-359，373-401，405- 412	B：2，C：2	176-187	103， 320	gbs1420			C：3，D：4	370-478	102， 319
gbs1429	Hypothetical protein matter		B：2，C：2	176-187	103， 320	gbs1442	Suppose thiamines biosynthesizing protein, ThiI	33-44，50-55，59-80，86-101，129-139，147-1 53，157-163，171-176，189-201，203-224，239 -245，257-262，281-287，290-297，304-320，3 22-331，334-350，372-390，396-401	L：28	71-88， 353-372	104， 321
gbs1452	Rp1T 50S ribosomal protein L20	5-11，15-24，26-33，40-47，75-88，95-103，10 5-112	E：2	17-30	105， 322	gbs1442	Suppose thiamines biosynthesizing protein, ThiI		L：28	71-88， 353-372	104， 321
gbs1452	Rp1T 50S ribosomal protein L20	5-11，15-24，26-33，40-47，75-88，95-103，10 5-112	E：2	17-30	105， 322	gbs1464	Ferrichrome abc transport albumen (permease)	5-11，16-39，46-54，62-82，100-107，111-124 ，126-150，154-165，167-183，204-238，245-2	F：4	8-16	106， 323

The streptococcus agalactiae antigen protein	The function of inferring (passing through homology)	The immunogenicity amino acid * * that infers	Each ORF and the clone that selects of screening number	The position (amino acid) in the immunogenicity zone of differentiating	Seq. ID (DNA, protein)
The streptococcus agalactiae antigen protein	The function of inferring (passing through homology)	The immunogenicity amino acid * * that infers	Each ORF and the clone that selects of screening number		Seq. ID (DNA, protein)			95，301-313，316-335
gbs1470	Conservative hypothetical protein matter	4-19，34-48，69-74，79-107，115-127，129-13 5，143-153，160-169，171-182	I：4	142-153	107， 324			95，301-313，316-335
gbs1470	Conservative hypothetical protein matter		I：4	142-153	107， 324	gbs1528	Conservative hypothetical protein matter	4-30，65-74，82-106，110-120，124-132，135- 140，146-175，179-184，190-196，217-223，22 8-233，250-267，275-292，303-315，322-332	I：7	174-186	108， 325
gbs1529	The binding peptide glycan albumen of inferring (LPXTG group)	9-16，29-41，47-57，68-84，87-109，113-119， 162-180，186-193，195-201，203-208，218-23 0，234-243，265-271，281-292，305-312，323- 332，341-347，349-363，368-374，383-390，39 5-410，434-440，446-452，455-464，466-473， 515-522，529-542，565-570，589-600，602-61 3，618-623，637-644，1019-1027，1238-1244， 1258-1264，1268-1276，1281-1292，1296-130 2	C：2	883-936	109， 326	gbs1528	Conservative hypothetical protein matter		I：7	174-186	108， 325
gbs1529			C：2	883-936	109， 326	gbs1531	UvrB excisionase ABC chain B (excinuclease ABC chain B)	10-17，23-32，39-44，54-72，75-81，88-111，1 38-154，160-167，178-185，201-210，236-252 ，327-334，336-342，366-376，388-400，410-4 30，472-482，493-526，552-558，586-592，598 -603，612-621，630-635，641-660	M：2	384-393	110， 327
gbs1533	Glutamine abc transport albumen, glutamine is conjugated protein	4-22，24-39，50-59，73-84，100-105，111-117 ，130-138，155-161，173-178，182-189，205-2 15，266-284，308-313，321-328，330-337，346 -363，368-374，388-395，397-405，426-434，4 53-459，482-492，501-507，509-515，518-523 ，527-544，559-590，598-612，614-629，646-6 59，663-684，686-694，698-721	I：4	445-461	111， 328	gbs1531	UvrB excisionase ABC chain B (excinuclease ABC chain B)		M：2	384-393	110， 327
gbs1533			I：4	445-461	111， 328	gbs1536	Hypothetical protein matter	14-22，27-33	E：10	3-17	112， 329
gbs1542	Oxydo-reductase, aldehyde/ketone reductase family	29-41，66-73，81-87，90-108，140-146，150-1 59，165-184，186-196，216-226，230-238，247 -253，261-269	I：13	126-140	113， 330	gbs1536	Hypothetical protein matter	14-22，27-33	E：10	3-17	112， 329
gbs1542	Oxydo-reductase, aldehyde/ketone reductase family		I：13	126-140	113， 330	gbs1547	Small protein matter, SmpB	5-12，16-25，27-33，36-45，60-68，83-88，103 -126	L：11	86-101	114， 331
gbs1565	Hypothetical protein matter	14-23，36-47，56-66，84-89，94-105，111-127 ，140-153，160-174，176-183，189-203，219-2 25，231-237，250-257	F：2，J：2， K：8，L：21	194-227	115， 332	gbs1547	Small protein matter, SmpB	5-12，16-25，27-33，36-45，60-68，83-88，103 -126	L：11	86-101	114， 331
gbs1565	Hypothetical protein matter		F：2，J：2， K：8，L：21	194-227	115， 332	gbs1586	Peptide acyl prolyl cis-trans isomerization enzyme, cyclophilin (cyclophilin) type	4-25，54-60，64-71，73-82，89-106，117-124， 157-169，183-188，199-210，221-232，236-24 4，255-264	E：3	58-98	116， 333

The streptococcus agalactiae antigen protein	The function of inferring (passing through homology)	The immunogenicity amino acid * * that infers	Each ORF and the clone that selects of screening number	The position (amino acid) in the immunogenicity zone of differentiating	Seq. ID (DNA, protein)
The streptococcus agalactiae antigen protein	The function of inferring (passing through homology)	The immunogenicity amino acid * * that infers	Each ORF and the clone that selects of screening number		Seq. ID (DNA, protein)	gbs1591	5-methylthioadenosine nucleosidase/S-acyl glycosides homocysteine nucleosidase (pfs)	13-19，26-36，41-53，55-71，77-84，86-108，1 14-135，157-172，177-183，187-194，208-213 ，218-226	I：18，L：2	110-125， 156-170	117， 334
gbs1632	Be similar to branched-chain amino acid abc transport albumen, amino acid is conjugated protein	5-24，63-69，77-85，94-112，120-137，140-14 6，152-159，166-172，179-187，193-199，206- 212，222-228，234-240，244-252，257-264，27 0-289，298-309，316-328，337-348，363-375	B：2，E：4， I：3	1-56， 340-352	118， 335	gbs1591			I：18，L：2	110-125， 156-170	117， 334
gbs1632			B：2，E：4， I：3	1-56， 340-352	118， 335	gbs1638	Amino acid permease	18-39，42-71，78-120，124-144，152-173，179 -189，199-209，213-222，228-258，269-304，3 29-361，364-372，374-389，396-441	E：8，G：9， H：9	313-327	119， 336
gbs1662	Conservative hypothetical protein matter	19-25，91-98，108-120，156-162，168-174，19 1-204，211-216，232-266，272-278，286-308， 316-321，327-333，344-355，358-364，384-39 1，395-428，464-476，487-495，497-511，544- 561，563-573，575-582，588-594	E：3，H：2	10-25， 322-338	120， 337	gbs1638	Amino acid permease		E：8，G：9， H：9	313-327	119， 336
gbs1662	Conservative hypothetical protein matter		E：3，H：2	10-25， 322-338	120， 337	gbs1666	SWI/SNF family helicase	14-26，32-49，51-57，59-72，80-91，102-112， 119-125，147-161，164-173，175-183，188-21 3，217-222，246-254，260-276，282-303，308- 318，321-328，333-350，352-359，371-378，39 2-401，407-414，416-443，448-463，471-484， 490-497，501-514，519-527，539-551，557-57 0，578-590，592-598，600-610，618-629，633- 647，654-667，676-689，702-709，718-726，72 8-737，741-760，764-780，786-795，808-826， 836-842，845-852，865-874，881-887，931-94 5，949-957，968-974，979-986，1003-1009，10 23-1029	F：4	90-103	121， 338
gbs1673	Conservative hypothetical protein matter	11-16，37-56，60-66，69-77，80-88，93-106，1 17-139，166-171	E：2	72-90	122， 339	gbs1666	SWI/SNF family helicase		F：4	90-103	121， 338
gbs1673	Conservative hypothetical protein matter	11-16，37-56，60-66，69-77，80-88，93-106，1 17-139，166-171	E：2	72-90	122， 339	gbs1695	The dihydroxyacetone kinase family protein	59-84，123-133，145-150，161-167，178-189	I：8	115-128	123， 340
gbs1754	Nickase ABC, A subunit (uvrA)	15-33，39-46，52-64，74-87，108-124，127-14 4，150-156，173-179，184-194，201-208，219- 236，243-269，272-295，302-309，343-349，35 6-361，370-379，405-411，414-423，430-451， 457-464，466-475，477-483，496-502，507-52 2，541-548，557-563，571-577，579-585，590- 605，626-642，650-662，671-691，704-710，75 1-769，775-781，786-791，794-829，851-858， 868-878，884-904，913-919，931-939	I：2	132-142	124， 341	gbs1695	The dihydroxyacetone kinase family protein	59-84，123-133，145-150，161-167，178-189	I：8	115-128	123， 340

The streptococcus agalactiae antigen protein	The function of inferring (passing through homology)	The immunogenicity amino acid * * that infers	Each ORF and the clone that selects of screening number	The position (amino acid) in the immunogenicity zone of differentiating	Seq. ID (DNA, protein)
The streptococcus agalactiae antigen protein	The function of inferring (passing through homology)	The immunogenicity amino acid * * that infers	Each ORF and the clone that selects of screening number		Seq. ID (DNA, protein)	gbs1760	Be similar to A/G specificity VITAMIN B4 transglucosylase	33-58，64-71，74-80，83-88，96-120，122-139 ，146-157，167-177，207-213，220-225，236-2 42，264-279，300-305，326-336，340-347，350 -360	K：8	97-115， 199-211	125， 342
gbs1777	The glycerine picked-up promotes protein, supposition	4-26，43-57，70-99，102-117，121-133，142-1 48，151-168，170-183，192-220，235-249，258 -279	E：4	30-41	126， 343	gbs1760	Be similar to A/G specificity VITAMIN B4 transglucosylase		K：8	97-115， 199-211	125， 342
gbs1777	The glycerine picked-up promotes protein, supposition		E：4	30-41	126， 343	gbs1783	Polyisoprene synthetic enzyme family protein	34-42，48-58，70-94，110-130，154-160，164- 172，178-183，195-203，211-222，229-250，25 6-261，274-284，286-292，312-323	I：3	222-233	127， 344
gbs1784	Abc transport albumen, the conjugated protein CydC of ATP	4-9，15-36，38-45，49-74，78-88，100-112，13 6-191，211-220，226-233，239-246，254-274， 287-307，316-322，342-353，356-366，373-37 8，384-393，405-431，449-457，459-468，487- 511，515-524，529-541，544-552，562-568，57 1-576	C：2，D：2	208-280	128， 345	gbs1783	Polyisoprene synthetic enzyme family protein		I：3	222-233	127， 344
gbs1784	Abc transport albumen, the conjugated protein CydC of ATP		C：2，D：2	208-280	128， 345	gbs1790	Hypothetical protein matter	10-27，31-37，39-54，71-108，124-143	A：23，C：6	2-107	129， 346
gbs1805	Be similar to excretory agnoprotein matter	16-27，38-57，64-70，90-102，104-113，116-1 37，160-166	A：197，C：2	1-80	130， 347	gbs1790	Hypothetical protein matter	10-27，31-37，39-54，71-108，124-143	A：23，C：6	2-107	129， 346
gbs1805	Be similar to excretory agnoprotein matter	16-27，38-57，64-70，90-102，104-113，116-1 37，160-166	A：197，C：2	1-80	130， 347	gbs1816	HD domain protein white matter	13-21，31-36，56-67，127-136，153-171，173- 180，184-200，214-222，225-231，239-263，26 7-273	F：8	135-159	131， 348
gbs1821	Be similar to 23S ribosome-RNA(rRNA) methyltransgerase	12-27，31-51，68-74，77-87，94-101，108-114 ，117-123，127-134，138-168，173-196，201-2 07，212-217，227-237，247-257，264-280	K：5	205-223	132， 349	gbs1816	HD domain protein white matter		F：8	135-159	131， 348
gbs1821	Be similar to 23S ribosome-RNA(rRNA) methyltransgerase		K：5	205-223	132， 349	gbs1823	Three family proteins	17-22，25-54，70-76，92-100	G：6，H：3	98-110	133， 350
gbs1834	Two-pack is experienced histidine kinase	7-29，40-50，60-67，87-96，105-111，119-164 ，172-199，206-212，220-227，237-259，272-2 79，282-293，295-309，313-319，321-328，345 -363，376-386	E：3，F：6	159-176	134， 351	gbs1823	Three family proteins	17-22，25-54，70-76，92-100	G：6，H：3	98-110	133， 350
gbs1834	Two-pack is experienced histidine kinase		E：3，F：6	159-176	134， 351	gbs1842	Transcribe the anti-factor, the Bg1G family of ending	4-19，24-30，36-43，50-68，71-89，93-106，14 1-152，154-172，179-197，199-215，229-239， 246-252，255-263，281-298，319-325，329-35 6，358-368，374-390，397-409，420-429，432- 444，450-456，459-475，483-494，496-502，52 0-528，532-556	I：19	362-377	135， 352
gbs1850	The transaldolase of supposing	18-25，40-62，77-85，91-97，105-116，123-13 3，139-184，189-197	G：2	122-140	136， 353	gbs1842	Transcribe the anti-factor, the Bg1G family of ending		I：19	362-377	135， 352

The streptococcus agalactiae antigen protein	The function of inferring (passing through homology)	The immunogenicity amino acid * * that infers	Each ORF and the clone that selects of screening number	The position (amino acid) in the immunogenicity zone of differentiating	Seq. ID (DNA, protein)
The streptococcus agalactiae antigen protein	The function of inferring (passing through homology)	The immunogenicity amino acid * * that infers	Each ORF and the clone that selects of screening number		Seq. ID (DNA, protein)	gbs1869	Phosphoglyceric kinase	4-49，52-58，62-70，79-105，109-133，142-15 0，163-168，206-214，220-228，233-240，243- 254，274-281，303-311，327-338，357-373，37 8-396，403-413，420-436，441-453，461-467， 475-481，484-498，506-512，514-521，523-52 9，562-579，589-595，598-603，615-648，714- 722，728-742，749-758，777-792，795-807	L：9	643-658	137， 354
gbs1875	Alkyl peroxide reductase enzyme (big subunit) and NADPH desaturase	8-27，37-48，51-56，72-79，87-106，120-138， 140-147，167-176，187-197，205-216，222-22 9，234-239，243-249，277-288，292-315，334- 343，347-353，363-391，398-404，430-447，46 1-467，478-492，498-507	F：3	456-470	138， 355	gbs1869	Phosphoglyceric kinase		L：9	643-658	137， 354
gbs1875			F：3	456-470	138， 355	gbs1879	Endopeptidase O (pepO)	5-12，18-24，59-69，80-93，95-109，119-125， 130-137，139-147，158-163，168-176，182-20 2，206-215，222-239，241-249，267-277，291- 298，311-318，321-327，338-344，348-355，37 3-386，393-406，411-417，434-443，446-465， 473-484，514-521，532-553，584-594	I：26	221-237	139， 356
gbs1893	2-Keto-3-deoxygluconokinase	4-14，27-34，50-58，63-72，79-106，109-114， 121-142，146-154，161-167，169-175，178-20 1，223-238，249-254，259-264，278-292，294- 312，319-330	F：8，K：9	167-191	140， 357	gbs1879	Endopeptidase O (pepO)		I：26	221-237	139， 356
gbs1893	2-Keto-3-deoxygluconokinase		F：8，K：9	167-191	140， 357	gbs1899	N-acetyl muramyl-L-L-Ala acyl ammonia enzyme, family's april protein	7-28，36-42，50-61，63-80，122-152，161-174 ，176-191	B：2，C：2， E：3	140-190	141， 358
gbs1907	The citrate carrier protein, CCS family	20-57，59-65，70-78，86-102，119-133，142-1 61，163-173，177-188，192-202，204-220，222 -236，240-253，279-319，326-331，337-383，3 90-399，406-412，420-427，431-438	I：2	381-395	142， 359	gbs1899		7-28，36-42，50-61，63-80，122-152，161-174 ，176-191	B：2，C：2， E：3	140-190	141， 358
gbs1907	The citrate carrier protein, CCS family		I：2	381-395	142， 359	gbs1924	Be similar to pneumonia histidine triad PROTEIN B precursor (C-terminal part)	13-18，28-34，37-43，50-59，75-81，83-97，10 5-121，139-147，200-206，209-227，231-247， 260-271，318-327，366-381，388-394，399-40 6	K：3	182-201	143， 360
gbs1925	Be similar to pneumonia histidine triad PROTEIN B precursor (N-terminal part)	6-29，37-43，51-56，70-77，82-102，110-119， 127-143，178-190，201-209，216-243，261-26 9，281-292，305-313，327-339，341-354，356- 373，391-397，423-429，438-445，450-478	A：2，B：5， C：12，D：57	21-314	144， 361	gbs1924			K：3	182-201	143， 360
gbs1925			A：2，B：5， C：12，D：57	21-314	144， 361	gbs1962	Conservative hypothetical protein matter	4-12，15-21，32-41，59-76，80-89，96-104	E：3	90-103	145， 362
gbs2008	Be similar to the C5A peptase, infer that peptidoglycan connects	9-28，30-41，44-54，69-74，77-82，90-97，104 -123，125-135，149-155，164-173，177-184，2	A：253， B：2，C：3，	3-82， 509-576	146， 363	gbs1962	Conservative hypothetical protein matter	4-12，15-21，32-41，59-76，80-89，96-104	E：3	90-103	145， 362

The streptococcus agalactiae antigen protein	The function of inferring (passing through homology)	The immunogenicity amino acid * * that infers	Each ORF and the clone that selects of screening number	The position (amino acid) in the immunogenicity zone of differentiating	Seq. ID (DNA, protein)
The streptococcus agalactiae antigen protein	The function of inferring (passing through homology)	The immunogenicity amino acid * * that infers	Each ORF and the clone that selects of screening number		Seq. ID (DNA, protein)		Albumen (LPXTG group)	17-226，230-235，238-244，258-272，282-297 ，300-305，309-315，317-322，327-336，348-3 62，368-374，380-387，400-411，414-424，451 -458，460-466，483-494，497-503，506-511，5 21-528，540-553，569-587，598-606，628-642 ，661-681，688-700，718-733，740-749，752-7 64，769-783，823-834，848-854，862-872，878 -884，886-898，915-920，938-951，954-961，9 63-972，982-989，996-1003，1010-1016，1021 -1032，1038-1044，1047-1057，1060-1070，10 79-1088，1094-1102，1117-1127，1129-1135， 1142-1153，1158-1204，1212-1229，1234-126 3，1269-1277，1308-1313，1327-1338，1344-1 376，1400-1415，1436-1443，1448-1458，1497 -1504，1511-1522，1544-1566	D：6，H：2
gbs2018	Infer that peptidoglycan connects protein (LPXTG group)	8-36，40-64，71-79，88-94，102-109，118-127 ，138-148，151-159，163-174，192-198，200-2 06，220-233，268-273，290-301，304-309，316 -323，331-349，378-391，414-420，427-437，4 55-475，494-510，541-547，549-555，616-640	A：132， B：6，C：13， D63，E：15， H：2，J：9， K：13	1-60， 55-139， 212-308， 386-458， 458-624	147， 364		Albumen (LPXTG group)		D：6，H：2
gbs2018	Infer that peptidoglycan connects protein (LPXTG group)		A：132， B：6，C：13， D63，E：15， H：2，J：9， K：13	1-60， 55-139， 212-308， 386-458， 458-624	147， 364	gbs2029	Hypothetical protein matter	16-31，35-42，70-77，91-101，120-130，132-1 40，143-153，185-190，195-202，215-222，228 -238，241-251，257-264，268-277，288-302，3 12-324，326-333，341-348，364-382，415-429 ，438-454，458-466，491-499，501-521	G：8	273-281	148， 365
gbs2042	Hypothetical protein matter	8-14，32-57，74-149，155-177，179-212，221- 266，271-296，304-324，329-346，349-359，36 8-401，413-419，426-454，465-478，493-510	L：11	466-490	149， 366	gbs2029	Hypothetical protein matter		G：8	273-281	148， 365
gbs2042	Hypothetical protein matter		L：11	466-490	149， 366	gbs2054	Dna mismatch is repaired protein HexA	22-28，33-51，64-89，96-119，126-132，138-1 46，152-159，161-169，172-179，193-198，205 -211，221-231，235-254，273-280，297-303，3 12-320，328-346，351-373，378-384，391-398 ，448-454，460-468，470-481，516-558，574-5 93，597-602，613-623，626-646，649-656，668 -673，675-683，696-708，715-722，724-739，7 45-751，759-777，780-804，816-822	E：8	102-113	150， 367
gbs2058	Hypothetical protein matter	12-28，41-91，98-107，112-120，125-131，151 -193，215-221，240-250，263-280	L：3	128-138	151， 368	gbs2054	Dna mismatch is repaired protein HexA		E：8	102-113	150， 367
gbs2058	Hypothetical protein matter		L：3	128-138	151， 368	gbs2060	Aspartoyl-tRNA synthetic enzyme (aspS)	16-24，32-38，46-62，68-81，90-105，127-133 ，144-150，160-166，178-184，186-202，210-2 19，232-240，252-258，264-273，293-324，337	I：3，L：12	96-109， 127-139	152， 369

The streptococcus agalactiae antigen protein	The function of inferring (passing through homology)	The immunogenicity amino acid * * that infers	Each ORF and the clone that selects of screening number	The position (amino acid) in the immunogenicity zone of differentiating	Seq. ID (DNA, protein)
The streptococcus agalactiae antigen protein	The function of inferring (passing through homology)	The immunogenicity amino acid * * that infers	Each ORF and the clone that selects of screening number		Seq. ID (DNA, protein)			-344，349-357，360-369，385-398，410-416，4 19-427，441-449，458-476，508-515，523-539 ，544-549，562-569，571-579
gbs2075	Hypothetical protein matter	19-25，28-34，56-61，85-97，110-116	M：2	39-53	153， 370
gbs2075	Hypothetical protein matter	19-25，28-34，56-61，85-97，110-116	M：2	39-53	153， 370	gbs2106	Unknown function protein/lipoprotein, supposition	4-37，41-50，62-72，91-97，99-109，114-125， 136-141，149-158，160-166，201-215	A：5，B：6， C：4，D：14， E：11，I：8， K：23	27-225	154， 371
gbs2118	Be similar to xanthoglobulin list phosphate dehydrogenase	15-31，44-51，96-105，122-130，149-157，162 -168，178-183，185-192，198-204，206-213，2 21-234，239-245，248-255，257-266，289-335 ，349-357，415-422，425-441，448-454，462-4 68	K：17	463-481	155， 372	gbs2106	Unknown function protein/lipoprotein, supposition		A：5，B：6， C：4，D：14， E：11，I：8， K：23	27-225	154， 371
gbs2118	Be similar to xanthoglobulin list phosphate dehydrogenase		K：17	463-481	155， 372	gbs2131	Abc transport albumen, permease protein, supposition	5-31，39-55，63-72，76-99，106-155，160-177 ，179-199，207-217，223-240，245-255，261-2 67，294-316，321-343，354-378，382-452，477 -488，529-536，555-569，584-591，593-612，6 20-627，632-640，647-654，671-680，698-704 ，723-730，732-750，769-775，781-788，822-8 52	I：2	505-525	156， 373
						gbs2131	Abc transport albumen, permease protein, supposition		I：2	505-525	156， 373
						ARF0112	Hypothetical protein matter	Do not have	F：6	3-18	157， 374
ARF0147	Hypothetical protein matter	4-14	E：3，I：3	12-24	158， 375	ARF0112	Hypothetical protein matter	Do not have	F：6	3-18	157， 374
ARF0147	Hypothetical protein matter	4-14	E：3，I：3	12-24	158， 375	ARF0532	Hypothetical protein matter	4-11，22-30	F：10	12-25	159， 376
ARF0534	Hypothetical protein matter	5-12	E：2，G：2	4-18	160， 377	ARF0532	Hypothetical protein matter	4-11，22-30	F：10	12-25	159， 376
ARF0534	Hypothetical protein matter	5-12	E：2，G：2	4-18	160， 377	ARF0557	Hypothetical protein matter	4-28	E：2，G：6， H：4	7-14	161， 378
ARF0862	Hypothetical protein matter	6-16	G：7，H：4	8-16	162， 379	ARF0557	Hypothetical protein matter	4-28	E：2，G：6， H：4	7-14	161， 378
ARF0862	Hypothetical protein matter	6-16	G：7，H：4	8-16	162， 379	ARF0891	Hypothetical protein matter	4-15，18-33	K：6	24-36	163， 380
ARF0895	Hypothetical protein matter	4-10，16-21	I：21	20-31	164， 381	ARF0891	Hypothetical protein matter	4-15，18-33	K：6	24-36	163， 380
ARF0895	Hypothetical protein matter	4-10，16-21	I：21	20-31	164， 381	ARF0943	Hypothetical protein matter	Do not have	C：2，K：9	6-19	165， 382
ARF0973	Hypothetical protein matter	11-18	D：2，G：3，	3-10	166，	ARF0943	Hypothetical protein matter	Do not have	C：2，K：9	6-19	165， 382

The streptococcus agalactiae antigen protein	The function of inferring (passing through homology)	The immunogenicity amino acid * * that infers	Each ORF and the clone that selects of screening number	The position (amino acid) in the immunogenicity zone of differentiating	Seq. ID (DNA, protein)
The streptococcus agalactiae antigen protein	The function of inferring (passing through homology)	The immunogenicity amino acid * * that infers	Each ORF and the clone that selects of screening number		Seq. ID (DNA, protein)				H：8，I：2， K：2		383
ARF0999	Hypothetical protein matter	13-24	B：4，K：3	3-15	167， 384				H：8，I：2， K：2		383
ARF0999	Hypothetical protein matter	13-24	B：4，K：3	3-15	167， 384	ARF1010	Hypothetical protein matter	15-27	K：2	7-16	168， 385
ARF1230	Hypothetical protein matter	11-16	K：11	1-15	169， 386	ARF1010	Hypothetical protein matter	15-27	K：2	7-16	168， 385
ARF1230	Hypothetical protein matter	11-16	K：11	1-15	169， 386	ARF1503	Hypothetical protein matter	4-16	E：13	9-21	170， 387
ARF1556	Hypothetical protein matter	4-24，40-48，54-67	F：2	22-39	171， 388	ARF1503	Hypothetical protein matter	4-16	E：13	9-21	170， 387
ARF1556	Hypothetical protein matter	4-24，40-48，54-67	F：2	22-39	171， 388	ARF1585	Hypothetical protein matter	6-30，34-55，62-68，78-106	I：5，J：4	68-74	172， 389
ARF1588	Hypothetical protein matter	Do not have	I：2	3-14	173， 390	ARF1585	Hypothetical protein matter	6-30，34-55，62-68，78-106	I：5，J：4	68-74	172， 389
ARF1588	Hypothetical protein matter	Do not have	I：2	3-14	173， 390	ARF1735	Hypothetical protein matter	9-19	I：13	6-21	174， 391
ARF1809	Hypothetical protein matter	4-17	H：2，L：17	1-9	175， 392	ARF1735	Hypothetical protein matter	9-19	I：13	6-21	174， 391
ARF1809	Hypothetical protein matter	4-17	H：2，L：17	1-9	175， 392	ARF1826	Hypothetical protein matter	5-30	I：6	1-8	176， 393
ARF1882	Hypothetical protein matter	4-16，23-46，51-56	K：23	45-55	177， 394	ARF1826	Hypothetical protein matter	5-30	I：6	1-8	176， 393
ARF1882	Hypothetical protein matter	4-16，23-46，51-56	K：23	45-55	177， 394	ARF1996	Hypothetical protein matter	Do not have	F：3	7-16	178， 395
CRF0123	Hypothetical protein matter	Do not have	F：32	2-14	179， 396	ARF1996	Hypothetical protein matter	Do not have	F：3	7-16	178， 395
CRF0123	Hypothetical protein matter	Do not have	F：32	2-14	179， 396	CRF0180	Hypothetical protein matter	4-36，43-65	E：6，G：6， H：12	50-62	180， 397
CRF0208	Hypothetical protein matter	10-30	I：2	14-21	181， 398	CRF0180	Hypothetical protein matter	4-36，43-65	E：6，G：6， H：12	50-62	180， 397
CRF0208	Hypothetical protein matter	10-30	I：2	14-21	181， 398	CRF0258	Hypothetical protein matter	9-17	I：2	1-10	182， 399
CRF0285	Hypothetical protein matter	4-12	F：2	3-16	183， 400	CRF0258	Hypothetical protein matter	9-17	I：2	1-10	182， 399
CRF0285	Hypothetical protein matter	4-12	F：2	3-16	183， 400	CRF0311	Hypothetical protein matter	4-15	H：4	5-23	184， 401
CRF0446	Hypothetical protein matter	Do not have	L：20	10-21	185， 402	CRF0311	Hypothetical protein matter	4-15	H：4	5-23	184， 401

The streptococcus agalactiae antigen protein	The function of inferring (passing through homology)	The immunogenicity amino acid * * that infers	Each ORF and the clone that selects of screening number	The position (amino acid) in the immunogenicity zone of differentiating	Seq. ID (DNA, protein)
The streptococcus agalactiae antigen protein	The function of inferring (passing through homology)	The immunogenicity amino acid * * that infers	Each ORF and the clone that selects of screening number		Seq. ID (DNA, protein)	CRF0455	Hypothetical protein matter	Do not have	F：5	6-16	186， 403
CRF0491	Hypothetical protein matter	4-29，31-38	G：4	2-14	187， 404	CRF0455	Hypothetical protein matter	Do not have	F：5	6-16	186， 403
CRF0491	Hypothetical protein matter	4-29，31-38	G：4	2-14	187， 404	CRF0520	Hypothetical protein matter	4-35	H：4	33-42	188， 405
CRF0530	Hypothetical protein matter	Do not have	G：13，H：8， K：3	2-17	189， 406	CRF0520	Hypothetical protein matter	4-35	H：4	33-42	188， 405
CRF0530	Hypothetical protein matter	Do not have	G：13，H：8， K：3	2-17	189， 406	CRF0570	Hypothetical protein matter	9-18，30-35	I：2	15-33	190， 407
CRF0649	Hypothetical protein matter	4-9	G：8，H：6	6-12	191， 408	CRF0570	Hypothetical protein matter	9-18，30-35	I：2	15-33	190， 407
CRF0649	Hypothetical protein matter	4-9	G：8，H：6	6-12	191， 408	CRF0853	Hypothetical protein matter	Do not have	I：6	3-17	192， 409
CRF0955	Hypothetical protein matter	12-21，37-44，52-61，72-80	E：7，L：44	38-48	193， 410	CRF0853	Hypothetical protein matter	Do not have	I：6	3-17	192， 409
CRF0955	Hypothetical protein matter	12-21，37-44，52-61，72-80	E：7，L：44	38-48	193， 410	CRF0983.1	Hypothetical protein matter	4-10，29-44，54-61，69-78	K：59	13-27	194， 411
CRF0983.2	Hypothetical protein matter	13-23，36-53	L：33	2-15	195， 412	CRF0983.1	Hypothetical protein matter	4-10，29-44，54-61，69-78	K：59	13-27	194， 411
CRF0983.2	Hypothetical protein matter	13-23，36-53	L：33	2-15	195， 412	CRF1083	Hypothetical protein matter	4-25，28-46，56-72，81-99，120-132，134-142 ，154-160	F：18	129-141	196， 413
CRF1095	Hypothetical protein matter	4-15，24-33，35-41，64-86	L：15	21-33	197， 414	CRF1083	Hypothetical protein matter	4-25，28-46，56-72，81-99，120-132，134-142 ，154-160	F：18	129-141	196， 413
CRF1095	Hypothetical protein matter	4-15，24-33，35-41，64-86	L：15	21-33	197， 414	CRF1212.1	Hypothetical protein matter	9-15	I：5	4-13	198， 415
CRF1212.2	Hypothetical protein matter	4-11，13-19，34-48	L：30	15-32	199， 416	CRF1212.1	Hypothetical protein matter	9-15	I：5	4-13	198， 415
CRF1212.2	Hypothetical protein matter	4-11，13-19，34-48	L：30	15-32	199， 416	CRF1290	Hypothetical protein matter	4-21	I：7	11-31	200， 417
CRF1383	Hypothetical protein matter	23-57	K：13	38-50	201， 418	CRF1290	Hypothetical protein matter	4-21	I：7	11-31	200， 417
CRF1383	Hypothetical protein matter	23-57	K：13	38-50	201， 418	CRF1416	Hypothetical protein matter	4-32	E：16，J：7	3-13	202， 419
CRF1500	Hypothetical protein matter	4-10，13-25，32-42，56-68，72-84	E：16	26-38	203， 420	CRF1416	Hypothetical protein matter	4-32	E：16，J：7	3-13	202， 419
CRF1500	Hypothetical protein matter	4-10，13-25，32-42，56-68，72-84	E：16	26-38	203， 420	CRF1513	Hypothetical protein matter	4-20，31-48，52-58，65-71，80-93，99-108，11 4-123	I：2	37-49	204， 421
CRF1518	Hypothetical protein matter	6-12，14-20	F：28	3-25	205， 422	CRF1513	Hypothetical protein matter	4-20，31-48，52-58，65-71，80-93，99-108，11 4-123	I：2	37-49	204， 421

The streptococcus agalactiae antigen protein	The function of inferring (passing through homology)	The immunogenicity amino acid * * that infers	Each ORF and the clone that selects of screening number	The position (amino acid) in the immunogenicity zone of differentiating	Seq. ID (DNA, protein)
The streptococcus agalactiae antigen protein	The function of inferring (passing through homology)	The immunogenicity amino acid * * that infers	Each ORF and the clone that selects of screening number		Seq. ID (DNA, protein)	CRF1663	Hypothetical protein matter	14-25，27-38	F：10	5-14	206， 423
CRF1667	Hypothetical protein matter	4-41，57-105，109-118，123-136，144-152	G：4	86-99	207， 424	CRF1663	Hypothetical protein matter	14-25，27-38	F：10	5-14	206， 423
CRF1667	Hypothetical protein matter	4-41，57-105，109-118，123-136，144-152	G：4	86-99	207， 424	CRF1832	Hypothetical protein matter	Do not have	E：5，L：8	6-19	208， 425
CRF1866	Hypothetical protein matter	Do not have	G：3，H：18	2-19	209， 426	CRF1832	Hypothetical protein matter	Do not have	E：5，L：8	6-19	208， 425
CRF1866	Hypothetical protein matter	Do not have	G：3，H：18	2-19	209， 426	CRF1892	Hypothetical protein matter	14-47	L：11	1-14	210， 427
CRF1942	Hypothetical protein matter	4-21，29-44	F：14	2-18	211， 428	CRF1892	Hypothetical protein matter	14-47	L：11	1-14	210， 427
CRF1942	Hypothetical protein matter	4-21，29-44	F：14	2-18	211， 428	CRF1992	Hypothetical protein matter	23-29	K：10	10-28	212， 429
CRF2047	Hypothetical protein matter	6-16，22-36	K：9	11-22	213， 430	CRF1992	Hypothetical protein matter	23-29	K：10	10-28	212， 429
CRF2047	Hypothetical protein matter	6-16，22-36	K：9	11-22	213， 430	CRF2050	Hypothetical protein matter	4-19，30-44	I：2	18-27	214， 431
CRF2096	Hypothetical protein matter	5-15，37-45，58-65	G：2	38-47	215， 432	CRF2050	Hypothetical protein matter	4-19，30-44	I：2	18-27	214， 431
CRF2096	Hypothetical protein matter	5-15，37-45，58-65	G：2	38-47	215， 432	CRF2113	Hypothetical protein matter	4-15，23-34	I：5	4-15	216， 433
NRF1311	Translocase, the C-terminal portions	30-36，44-54，79-85，101-114，138-152，154- 164，170-175，179-200，213-220，223-240，24 3-255，258-264，268-284	F：3	10-28	217， 434	CRF2113	Hypothetical protein matter	4-15，23-34	I：5	4-15	216， 433

Table 1B: the peptide that immunogenic protein that aminoacid sequence identification is differentiated and bacterium surface displaying are differentiated

Streptococcus agalactiae antigen protein (newly)	Same area	Peptide sequence	The peptide title	The protein that BSD differentiates	Immunogenicity zone (aa)	Serial ID (DNA, protein)
Streptococcus agalactiae antigen protein (newly)	Same area	Peptide sequence	The peptide title	The protein that BSD differentiates	Immunogenicity zone (aa)	Serial ID (DNA, protein)	gbs0384	210-226 738-753	MEYKGNFSQKTINRFKS QTQRSGKINTDFMRQL	gbs0995.1 gbs0995.2	gbs0995 gbs0995	210-226 738-753	435，449
gbs0393	326-344 326-348 338-354 371-392 801-809 877-901	VKTIGYGKLTGKVNHHYVA VKTIGYGKLTGKVNHHYVANKDG VNHHYVANKDGSVTAFV AAVNQNIVFRVLTKDGRPIFEK TVIKKGTNL VTHTTEKSKPVEPQKATPKAPAKGL	gbs0986.2 gbs1143.1 gbs0986.3 gbs1143.2 gbs0986.4 gbs0986.5	gbs0986 gbs1143 gbs0986 gbs1143 gbs0986 gbs0986	326-344 327-349 338-354 372-393 801-809 877-901	436，450	gbs0384	210-226 738-753	MEYKGNFSQKTINRFKS QTQRSGKINTDFMRQL	gbs0995.1 gbs0995.2	gbs0995 gbs0995	210-226 738-753	435，449
gbs0393	326-344 326-348 338-354 371-392 801-809 877-901		gbs0986.2 gbs1143.1 gbs0986.3 gbs1143.2 gbs0986.4 gbs0986.5	gbs0986 gbs1143 gbs0986 gbs1143 gbs0986 gbs0986	326-344 327-349 338-354 372-393 801-809 877-901	436，450	gbs0396	893-906	RQELLTPTQLSKLQ	gbs0983.1	gbs0983	893-906	437，451
gbs0407	51-69	VRYDKLEALVAYHGAKSAS	gbs0972.1	gbs0972	51-69	438，452	gbs0396	893-906	RQELLTPTQLSKLQ	gbs0983.1	gbs0983	893-906	437，451
gbs0407	51-69	VRYDKLEALVAYHGAKSAS	gbs0972.1	gbs0972	51-69	438，452	gbs0408	110-125	HQPNRIYLTDKLVPYI	gbs0971.1	gbs0971	110-125	439，453
gbs0410	291-305	QSIKQHDKEKLRTVL	gbs0969.1	gbs0969	291-305	440，454	gbs0408	110-125	HQPNRIYLTDKLVPYI	gbs0971.1	gbs0971	110-125	439，453
gbs0410	291-305	QSIKQHDKEKLRTVL	gbs0969.1	gbs0969	291-305	440，454	gbs0714	210-226 738-753	MEYKGNFSQKTINRFKS QTQRSGKINTDFMRQL	gbs0995.1 gbs0995.2	gbs0995 gbs0995	210-226 738-753	441，455
gbs0723	326-344 326-348 338-354 371-392 801-809 877-901	VKTIGYGKLTGKVNHHYVA VKTIGYGKLTGKVNHHYVANKDG VNHHYVANKDGSVTAFV AAVNQNIVFRVLTKDGRPIFEK TVIKKGTNL VTHTTEKSKPVEPQKATPKAPAKGL	gbs0986.2 gbs1143.1 gbs0986.3 gbs1143.2 gbs0986.4 gbs0986.5	gbs0986 gbs1143 gbs0986 gbs1143 gbs0986 gbs0986	326-344 327-349 338-354 372-393 801-809 877-901	442，456	gbs0714	210-226 738-753	MEYKGNFSQKTINRFKS QTQRSGKINTDFMRQL	gbs0995.1 gbs0995.2	gbs0995 gbs0995	210-226 738-753	441，455
gbs0723	326-344 326-348 338-354 371-392 801-809 877-901		gbs0986.2 gbs1143.1 gbs0986.3 gbs1143.2 gbs0986.4 gbs0986.5	gbs0986 gbs1143 gbs0986 gbs1143 gbs0986 gbs0986	326-344 327-349 338-354 372-393 801-809 877-901	442，456	gbs0726	893-906	RQELLTPTQLSKLQ	gbs0983.1	gbs0983	893-906	443，457
gbs0737	51-69	VRYDKLEALVAYHGAKSAS	gbs0972.1	gbs0972	51-69	444，458	gbs0726	893-906	RQELLTPTQLSKLQ	gbs0983.1	gbs0983	893-906	443，457
gbs0737	51-69	VRYDKLEALVAYHGAKSAS	gbs0972.1	gbs0972	51-69	444，458	gbs0738	110-125	HQPNRIYLTDKLVPYI	gbs0971.1	gbs0971	110-125	445，459
gbs0740	291-305	QSIKQHDKEXLRTVL	gbs0969.1	gbs0969	291-305	446，460	gbs0738	110-125	HQPNRIYLTDKLVPYI	gbs0971.1	gbs0971	110-125	445，459
gbs0740	291-305	QSIKQHDKEXLRTVL	gbs0969.1	gbs0969	291-305	446，460	gbs0897	32-44	EGDVLLEIMSDKT	gbs0898.1	gbs0898	32-44	447，461
gbs 0966	399-410	PGLTVEEKFVTF	gbs0144.1	gbs0144	420-431	448，462	gbs0897	32-44	EGDVLLEIMSDKT	gbs0898.1	gbs0898	32-44	447，461

The table 2. epi-position serology of human serum

Peptide	Positive	Amino acid from	Amino acid extremely	Seq ID
Peptide	Positive	Amino acid from	Amino acid extremely	Seq ID	gbs0012.1	++	120	143	218
gbs0012.2	+	138	161	218	gbs0012.1	++	120	143	218
gbs0012.2	+	138	161	218	gbs0012.3	+	156	179	218
gbs0016.2	+++	110	129	219	gbs0012.3	+	156	179	218
gbs0016.2	+++	110	129	219	gbs0016.3	+	168	184	219

gbs0048.1	+	74	90	222
gbs0048.1	+	74	90	222	gbs0053.1	+++	759	773	223
gbs0061.1	+++	237	260	224	gbs0053.1	+++	759	773	223
gbs0061.1	+++	237	260	224	gbs0084.1	+	265	284	225
gbs0107.1	++	65	74	226	gbs0084.1	+	265	284	225
gbs0107.1	++	65	74	226	gbs0108.1	++	41	50	227
gbs0123.1	+	163	174	229	gbs0108.1	++	41	50	227
gbs0123.1	+	163	174	229	gbs0127.1	++	26	37	230
gbs0183.1	+	174	189	232	gbs0127.1	++	26	37	230
gbs0183.1	+	174	189	232	gbs0235.1	++	240	256	234
gbs0260.1	+	285	297	236	gbs0235.1	++	240	256	234
gbs0260.1	+	285	297	236	gbs0286.1	+	238	247	238
gbs0288.1	+	491	519	239	gbs0286.1	+	238	247	238
gbs0288.1	+	491	519	239	gbs0437.1	++	114	140	243
gbs0539.1	+	267	284	250	gbs0437.1	++	114	140	243
gbs0539.1	+	267	284	250	gbs0579.1	+	439	453	252
gbs0580.1	++	162	178	253	gbs0579.1	+	439	453	252
gbs0580.1	++	162	178	253	gbs0628.1	++	347	364	254
gbs0632.1	+++	699	715	255	gbs0628.1	++	347	364	254
gbs0632.1	+++	699	715	255	gbs0634.1	+	60	71	256
gbs0667.1	++	244	257	257	gbs0634.1	+	60	71	256
gbs0667.1	++	244	257	257	gbs0672.1	+	44	63	258
gbs0672.2	+	57	76	258	gbs0672.1	+	44	63	258
gbs0672.2	+	57	76	258	gbs0785.1	+	185	196	260
gbs0851.1	+	119	129	263	gbs0785.1	+	185	196	260
gbs0851.1	+	119	129	263	gbs0896.1	++	182	195	266
gbs0898.1	++	32	44	267	gbs0896.1	++	182	195	266
gbs0898.1	++	32	44	267	gbs0898.2	+	424	442	267
gbs0904.1	+	247	256	268	gbs0898.2	+	424	442	267
gbs0904.1	+	247	256	268	gbs0918.1	++	678	694	269
gbs0918.2	+	785	805	269	gbs0918.1	++	678	694	269

gbs0918.4	+	55	77	269
gbs0918.4	+	55	77	269	gbs0918.5	+++	72	94	269
gbs0995.1	+	210	226	281	gbs0918.5	+++	72	94	269
gbs0995.1	+	210	226	281	gbs1087.3	+	37	59	289
gbs1165.1	+	13	29	296	gbs1087.3	+	37	59	289
gbs1165.1	+	13	29	296	gbs1816.1	+	136	159	348
gbs1821.1	+	205	222	349	gbs1816.1	+	136	159	348
gbs1821.1	+	205	222	349	gbs1823.1	+	99	110	350
gbs1834.1	+	160	176	351	gbs1823.1	+	99	110	350
gbs1834.1	+	160	176	351	gbs1875.1	+	457	470	355
gbs1879.1	+	221	237	356	gbs1875.1	+	457	470	355
gbs1879.1	+	221	237	356	gbs1893.1	+	167	190	357
gbs1925.1	+	96	120	361	gbs1893.1	+	167	190	357
gbs1925.1	+	96	120	361	gbs2018.3	+++	399	417	364
gbs2018.4	+++	503	519	364	gbs2018.3	+++	399	417	364
gbs2018.4	+++	503	519	364	gbs2018.5	+++	544	563	364
gbs2106.2	+	46	68	371	gbs2018.5	+++	544	563	364
gbs2106.2	+	46	68	371	gbs2106.7	+	159	183	371
gbs2106.8	+	184	198	371	gbs2106.7	+	159	183	371
gbs2106.8	+	184	198	371	gbs2118.1	++	463	481	372

Table 3: the gene distribution in the streptococcus agalactiae strain

ORF	General title	Gene distribution (50 existence)	Amino acid replacement (serotype IA strain)	Seq. ID (DNA, protein)
ORF	General title	Gene distribution (50 existence)	Amino acid replacement (serotype IA strain)	Seq. ID (DNA, protein)	gbs0012	Be similar to β-Nei Xiananmei a little	44/44	n.d.	1，218
gbs0016	Glucan-binding protein B (streptococcus mutans)	40/44	0/224	2，219	gbs0012	Be similar to β-Nei Xiananmei a little	44/44	n.d.	1，218
gbs0016	Glucan-binding protein B (streptococcus mutans)	40/44	0/224	2，219	gbs0024	Ribose phosphoric acid formyl G-NH2	46/46	10/228	3，220
gbs0031	The surface immunogenic protein	46/46	1/225	4，221	gbs0024	Ribose phosphoric acid formyl G-NH2	46/46	10/228	3，220
gbs0031	The surface immunogenic protein	46/46	1/225	4，221	gbs0048	Unknown	30/46	0/61	5，222
gbs0053	Aldehyde-alcoholdehydrogenase (adhE)	45/45	0/224	6，223	gbs0048	Unknown	30/46	0/61	5，222
gbs0053	Aldehyde-alcoholdehydrogenase (adhE)	45/45	0/224	6，223	gbs0061	Rp1B ribosomal protein L2	46/46	0/218	7，224
gbs0084	The RNA polymerase that DNA instructs, α-subunit (rpoA)	45/45	0/207	8，225	gbs0061	Rp1B ribosomal protein L2	46/46	0/218	7，224
gbs0084	The RNA polymerase that DNA instructs, α-subunit (rpoA)	45/45	0/207	8，225	gbs0107	Conservative hypothetical protein matter	46/46	0/235	9，226
gbs0108	Deoxyuridine 5 '-triphosphopyridine nucleotide lytic enzyme	44/44	0/125	10，227	gbs0107	Conservative hypothetical protein matter	46/46	0/235	9，226
gbs0108	Deoxyuridine 5 '-triphosphopyridine nucleotide lytic enzyme	44/44	0/125	10，227	gbs0113	Ribose abc transport albumen	44/45	0/227	11，228
gbs0123	Be similar to argininosuccinate synthetase	44/44	0/184	12，229	gbs0113	Ribose abc transport albumen	44/45	0/227	11，228
gbs0123	Be similar to argininosuccinate synthetase	44/44	0/184	12，229	gbs0127	RpmV 50S ribosomal protein L28	46/46	0/40	13，230
gbs0144	Oligopeptides abc transport albumen, the substrate combination	45/45	0/282	14，231	gbs0127	RpmV 50S ribosomal protein L28	46/46	0/40	13，230
gbs0144		45/45	0/282	14，231	gbs0183	Membrane protein, supposition	44/44	0/223	15，232
gbs0184	Oligopeptides abc transport albumen, the oligopeptides bonded	46/46	1/203	16，233	gbs0183	Membrane protein, supposition	44/44	0/223	15，232
gbs0184		46/46	1/203	16，233	gbs0235	Glycine hercynine/carnitine/choline ABC translocator	46/46	0/219	17，234
gbs0255	Conservative hypothetical protein matter	46/46	0/180	18，235	gbs0235	Glycine hercynine/carnitine/choline ABC translocator	46/46	0/219	17，234
gbs0255	Conservative hypothetical protein matter	46/46	0/180	18，235	gbs0260	Glycyl-tRNA synthetase (β-subunit)	46/46	0/209	19，236
gbs0268	Transketolase (tkt)	46/46	0/208	20，237	gbs0260	Glycyl-tRNA synthetase (β-subunit)	46/46	0/209	19，236
gbs0268	Transketolase (tkt)	46/46	0/208	20，237	gbs0286	NH3-dependency NAD+ synthetic enzyme	45/45	0/191	21，238
gbs0288	Be similar to penicillin-binding protein 1A	45/45	0/212	22，239	gbs0286	NH3-dependency NAD+ synthetic enzyme	45/45	0/191	21，238
gbs0288	Be similar to penicillin-binding protein 1A	45/45	0/212	22，239	gbs0343	Seryl-tRNA synthetase (serS)	46/46	0/228	23，240
gbs0428	Be similar to fibrinogen binding protein, the peptidoglycan of supposition connects albumen (LPXTG group)	45/46	1/126	25，242	gbs0343	Seryl-tRNA synthetase (serS)	46/46	0/228	23，240
gbs0428		45/46	1/126	25，242	gbs0437	Glucose-6-phosphate isomerase (pgi)	45/45	0/232	26，243
gbs0460	Decarboxylase	46/46	1/81	27，244	gbs0437	Glucose-6-phosphate isomerase (pgi)	45/45	0/232	26，243
gbs0460	Decarboxylase	46/46	1/81	27，244	gbs0465	Oxydo-reductase	46/46	0/126	28，245
gbs0489	Transacetylase, GNAT family	45/45	3/144	30，247	gbs0465	Oxydo-reductase	46/46	0/126	28，245
gbs0489	Transacetylase, GNAT family	45/45	3/144	30，247	gbs0492	The gbs0492 Valyl-tRNA synthetase	44/44	3/125	31，248
gbs0538	Amino acid abc transport albumen (ATP is conjugated protein)	46/46	0/214	32，249	gbs0492	The gbs0492 Valyl-tRNA synthetase	44/44	3/125	31，248
gbs0538		46/46	0/214	32，249	gbs0539	Be similar to mannose-phosphate mutase	46/46	0/244	33，250
gbs0555	Beta-lactam resistance factor (fibA)	46/46	0/218	34，251	gbs0539	Be similar to mannose-phosphate mutase	46/46	0/244	33，250
gbs0555	Beta-lactam resistance factor (fibA)	46/46	0/218	34，251	gbs0579	Pepx	46/46	0/218	35，252
gbs0580	Zinc abc transport albumen, zinc is in conjunction with adhering to lipoprotein	45/45	2/235	36，253	gbs0579	Pepx	46/46	0/218	35，252
gbs0580		45/45	2/235	36，253	gbs0628	Cell walls surface anchoring family protein (IPxTG)	42/44	0/219	37，254
gbs0632	Cell walls surface anchoring family protein, (the FPKTG group) of supposition	44/45	0/238	38，255	gbs0628	Cell walls surface anchoring family protein (IPxTG)	42/44	0/219	37，254
gbs0632		44/45	0/238	38，255	gbs0667	Regulate protein, supposition, block	44/44	0/229	40，257
gbs0672	Transcriptional regulatory (Gnt R family)	43/43	0/203	41，258	gbs0667	Regulate protein, supposition, block	44/44	0/229	40，257
gbs0672	Transcriptional regulatory (Gnt R family)	43/43	0/203	41，258	gbs0687	Agnoprotein matter	45/45	0/149	42，259
gbs0785	Be similar to penicillin-binding protein 2B	45/45	0/218	43，260	gbs0687	Agnoprotein matter	45/45	0/149	42，259
gbs0785	Be similar to penicillin-binding protein 2B	45/45	0/218	43，260	gbs0828	Agnoprotein matter	46/46	1/120	45，262
gbs0851	Hypothetical protein matter	46/46	0/140	46，263	gbs0828	Agnoprotein matter	46/46	1/120	45，262
gbs0851	Hypothetical protein matter	46/46	0/140	46，263	gbs0865	Gbs0865 the unknown	44/44	0/241	47，264
gbs0890	Exonuclease RexB (rexB)	46/46	0/232	48，265	gbs0865	Gbs0865 the unknown	44/44	0/241	47，264
gbs0890	Exonuclease RexB (rexB)	46/46	0/232	48，265	gbs0896	Be similar to the 3-acetoin dehydrogenase	46/46	0/239	49，266
gbs0898	The 3-acetoin dehydrogenase, thymus pyrimidine PPi complies with	45/45	0/180	50，267	gbs0896	Be similar to the 3-acetoin dehydrogenase	46/46	0/239	49，266

ORF	General title	Gene distribution (50 existence)	Amino acid replacement (serotype IA strain)	Seq. ID (DNA, protein)
ORF	General title	Gene distribution (50 existence)	Amino acid replacement (serotype IA strain)	Seq. ID (DNA, protein)		Bad property
gbs0904	Phosphoglucomutase/mannose-phosphate mutase family protein	46/46	0/169	51，268		Bad property
gbs0904	Phosphoglucomutase/mannose-phosphate mutase family protein	46/46	0/169	51，268	gbs0918	Be similar to histidine triad albumen a little, the lipoprotein of supposition	45/45	1/209	52，269
gbs0931	Pyruvate kinase	46/46	0/185	53，270	gbs0918		45/45	1/209	52，269
gbs0931	Pyruvate kinase	46/46	0/185	53，270	gbs0947	Similar in appearance to the L-serum lactic dehydrogenase	46/46	0/233	54，271
gbs0948	DNA gyrase (gyrase), A subunit (gyrA)	44/44	0/172	55，272	gbs0947	Similar in appearance to the L-serum lactic dehydrogenase	46/46	0/233	54，271
gbs0948	DNA gyrase (gyrase), A subunit (gyrA)	44/44	0/172	55，272	gbs1035	Conservative hypothetical protein matter	46/46	0/210	69，286
gbs1066	Gbs1066 the unknown	17/46	2/92	71，288	gbs1035	Conservative hypothetical protein matter	46/46	0/210	69，286
gbs1066	Gbs1066 the unknown	17/46	2/92	71，288	gbs1087	High multiple binding peptide glycan albumen (LPXTG group)	42/45	n.d.	72，289
gbs1103	Abc transport albumen (ATP is conjugated protein)	46/46	1/165	73，290	gbs1087	High multiple binding peptide glycan albumen (LPXTG group)	42/45	n.d.	72，289
gbs1103	Abc transport albumen (ATP is conjugated protein)	46/46	1/165	73，290	gbs1116	Xanthine permease (pbuX)	45/45	1/170	74，291
gbs1158	Be similar to oxydo-reductase	44/44	1/170	78，295	gbs1116	Xanthine permease (pbuX)	45/45	1/170	74，291
gbs1158	Be similar to oxydo-reductase	44/44	1/170	78，295	gbs1165	Disulfurase (iscS-1)	43/43	0/148#	79，296
gbs1195	Staphylokinase and streptokinase	45/45	60/142	80，297	gbs1165	Disulfurase (iscS-1)	43/43	0/148#	79，296
gbs1195	Staphylokinase and streptokinase	45/45	60/142	80，297	gbs1209	ATP dependent DNA helicase PcrA	43/44	1/94#	81，298
gbs1214	Conservative supposition protein	43/46	0/97	82，299	gbs1209	ATP dependent DNA helicase PcrA	43/44	1/94#	81，298
gbs1214	Conservative supposition protein	43/46	0/97	82，299	gbs1260	Abc transport albumen, ATP is conjugated protein	44/46	1/198	84，301
gbs1306	Ln conjugated protein (Spellerberg, people such as B 1999)	45/46	0/215	87，304	gbs1260	Abc transport albumen, ATP is conjugated protein	44/46	1/198	84，301
gbs1306	Ln conjugated protein (Spellerberg, people such as B 1999)	45/46	0/215	87，304	gbs1307	Lmb ln mating surface albumen	45/45	n.d.	88，305
gbs1308	The C5a peptase, real frameshit	46/46	0/205	89，306	gbs1307	Lmb ln mating surface albumen	45/45	n.d.	88，305
gbs1308	The C5a peptase, real frameshit	46/46	0/205	89，306	gbs1309	Hypothetical protein matter	44/46	0/214	90，307
gbs1356	The peptidoglycan of inferring connects albumen (LPXTG group)-agglutinin receptor	20/46	50/211#	94，311	gbs1309	Hypothetical protein matter	44/46	0/214	90，307
gbs1356		20/46	50/211#	94，311	gbs1376	Be similar to ATP dependency C1p proteolytic enzyme (ATP binding subunit), C1pL	45/45	0/197	95，312
gbs1377	Be similar to homocysteine S-methyltransgerase	45/45	0/55	96，313	gbs1376		45/45	0/197	95，312
gbs1377	Be similar to homocysteine S-methyltransgerase	45/45	0/55	96，313	gbs1386	-hydroxy-3-methylglutaryl A synthase	44/44	0/219	97，314
gbs1390	Gbs1390 the unknown	43/43	0/198	98，315	gbs1386	-hydroxy-3-methylglutaryl A synthase	44/44	0/219	97，314
gbs1390	Gbs1390 the unknown	43/43	0/198	98，315	gbs1391	Gbs1391 the unknown	44/44	0/214	99，316
gbs1403	Be similar to 5 '-phosphonuclease, the binding peptide glycan albumen (LPXTN) of supposition	45/45	3/189	100，317	gbs1391	Gbs1391 the unknown	44/44	0/214	99，316
gbs1403		45/45	3/189	100，317	gbs1408	Be similar to abc transport albumen (ATP is conjugated protein)	45/45	0/205	101，318
gbs1429	Agnoprotein matter	46/46	1/193	103，320	gbs1408		45/45	0/205	101，318
gbs1429	Agnoprotein matter	46/46	1/193	103，320	gbs1452	Rp1T 50S ribosomal protein L 20	46/46	0/101	105，322
gbs1464	Ferrichrome abc transport albumen (permease)	44/44	2/232	106，323	gbs1452	Rp1T 50S ribosomal protein L 20	46/46	0/101	105，322
gbs1464	Ferrichrome abc transport albumen (permease)	44/44	2/232	106，323	gbs1470	Conservative hypothetical protein matter	46/46	2/164	107，324
gbs1528	Conservative hypothetical protein matter	45/45	0/213	108，325	gbs1470	Conservative hypothetical protein matter	46/46	2/164	107，324
gbs1528	Conservative hypothetical protein matter	45/45	0/213	108，325	gbs1531	UvrB exonuclease ABC B chain	45/45	0/108	110，327
gbs1533	Glutamine abc transport albumen, glutamine is conjugated protein	44/44	0/166	111，328	gbs1531	UvrB exonuclease ABC B chain	45/45	0/108	110，327
gbs1533		44/44	0/166	111，328	gbs1542	Oxydo-reductase, aldehyde/ketone reductase family	45/45	1/219	113，330
gbs1565	Hypothetical protein matter	43/43	1/218	115，332	gbs1542	Oxydo-reductase, aldehyde/ketone reductase family	45/45	1/219	113，330

ORF	General title	Gene distribution (50 existence)	Amino acid replacement (serotype IA strain)	Seq. ID (DNA, protein)
ORF	General title	Gene distribution (50 existence)	Amino acid replacement (serotype IA strain)	Seq. ID (DNA, protein)	gbs1586	The peptide prolyl is suitable-the trans-isomerism enzyme, and the cyclophilin type	45/45	1/227	116，333
gbs1591	5-methylthioadenosine nucleosidase/S-adenosyl homocysteine	45/45	0/203	117，334	gbs1586		45/45	1/227	116，333
gbs1591	5-methylthioadenosine nucleosidase/S-adenosyl homocysteine	45/45	0/203	117，334	gbs1632	Be similar to branched-chain amino acid abc transport albumen, amino acid is conjugated protein	45/45	0/223	118，335
gbs1638	Amino acid permease	45/45	0/100	119，336	gbs1632		45/45	0/223	118，335
gbs1638	Amino acid permease	45/45	0/100	119，336	gbs1662	Conservative hypothetical protein matter	45/45	0/213	120，337
gbs1666	SWI/SNF family helicase	45/45	0/200	121，338	gbs1662	Conservative hypothetical protein matter	45/45	0/213	120，337
gbs1666	SWI/SNF family helicase	45/45	0/200	121，338	gbs1673	Conservative hypothetical protein matter	45/45	0/147	122，339
gbs1695	The dihydroxyacetone kinase family protein	43/43	1/165	123，340	gbs1673	Conservative hypothetical protein matter	45/45	0/147	122，339
gbs1695	The dihydroxyacetone kinase family protein	43/43	1/165	123，340	gbs1754	Nucleic acid excisionase ABC, A subunit (uvrA)	43/43	0/224	124，341
gbs1760	Be similar to A/G specificity VITAMIN B4 and change glucokinase	46/46	0/181	125，342	gbs1754	Nucleic acid excisionase ABC, A subunit (uvrA)	43/43	0/224	124，341
gbs1760		46/46	0/181	125，342	gbs1777	The glycerine picked-up promotes albumen, supposition	43/43	0/199	126，343
gbs1783	Polyisoprene synthetic enzyme family protein	45/45	0/217	127，344	gbs1777	The glycerine picked-up promotes albumen, supposition	43/43	0/199	126，343
gbs1783	Polyisoprene synthetic enzyme family protein	45/45	0/217	127，344	gbs1784	Abc transport albumen, ATP is conjugated protein, CydC	45/45	1/220	128，345
gbs1790	Agnoprotein matter	41/43	3/75#	129，346	gbs1784	Abc transport albumen, ATP is conjugated protein, CydC	45/45	1/220	128，345
gbs1790	Agnoprotein matter	41/43	3/75#	129，346	gbs1805	Be similar to excretory agnoprotein matter	45/45	0/66	130，347
gbs1816	HD domain protein white matter	43/43	1/176	131，348	gbs1805	Be similar to excretory agnoprotein matter	45/45	0/66	130，347
gbs1816	HD domain protein white matter	43/43	1/176	131，348	gbs1821	Be similar to 23S ribosome-RNA(rRNA) methyltransgerase	43/43	2/155#	132，349
gbs1834	Two-pack is experienced histidine kinase	44/44	0/213	134，351	gbs1821	Be similar to 23S ribosome-RNA(rRNA) methyltransgerase	43/43	2/155#	132，349
gbs1834	Two-pack is experienced histidine kinase	44/44	0/213	134，351	gbs1842	Transcribe anti-son, the Bg1G family of ending	43/43	0/208	135，352
gbs1850	Suppose transaldolase	44/44	0/194	136，353	gbs1842	Transcribe anti-son, the Bg1G family of ending	43/43	0/208	135，352
gbs1850	Suppose transaldolase	44/44	0/194	136，353	gbs1875	Alkyl peroxide reductase enzyme (big subunit) and nadh dehydrogenase	46/46	0/192	138，355
gbs1879	Endopeptidase O (pepO)	43/43	0/135	139，356	gbs1875		46/46	0/192	138，355
gbs1879	Endopeptidase O (pepO)	43/43	0/135	139，356	gbs1893	2-Keto-3-deoxygluconokinase	36/46	0/228	140，357
gbs1899	N-acetyl muramyl-L-ala amide enzyme, family's april protein	43/43	0/149	141，358	gbs1893	2-Keto-3-deoxygluconokinase	36/46	0/228	140，357
gbs1899	N-acetyl muramyl-L-ala amide enzyme, family's april protein	43/43	0/149	141，358	gbs1907	The citrate carrier proteins, CCS family	43/43	0/219	142，359
gbs1925	Be similar to pneumonia histidine triad PROTEIN B precursor (N-terminal portions)	43/43	0/103	144，361	gbs1907	The citrate carrier proteins, CCS family	43/43	0/219	142，359
gbs1925		43/43	0/103	144，361	gbs1962	Conservative hypothetical protein matter	28/46	0/136	145，362
gbs2008	Be similar to the C5A peptase, the peptidoglycan of supposition connects albumen (LPXTG group)	43/43	n.d.	146，363	gbs1962	Conservative hypothetical protein matter	28/46	0/136	145，362
gbs2008		43/43	n.d.	146，363	gbs2018	The peptidoglycan of inferring connects albumen (LPXTG group)	43/45	0/104	147，364
gbs2029	Agnoprotein matter	44/44	1/238	148，365	gbs2018		43/45	0/104	147，364
gbs2029	Agnoprotein matter	44/44	1/238	148，365	gbs2054	Dna mismatch is repaired albumen HexA	46/46	0/206	150，367
gbs2060	Aspartyl-tRNA synthetase (aspS)	46/46	2/211	152，369	gbs2054	Dna mismatch is repaired albumen HexA	46/46	0/206	150，367
gbs2060	Aspartyl-tRNA synthetase (aspS)	46/46	2/211	152，369	gbs2106	Unknown function protein/lipoprotein, supposition	44/44	0/160	154，371
gbs2118	Be similar to xanthoglobulin list phosphate dehydrogenase	43/43	0/113	155，372	gbs2106	Unknown function protein/lipoprotein, supposition	44/44	0/160	154，371
gbs2118	Be similar to xanthoglobulin list phosphate dehydrogenase	43/43	0/113	155，372	gbs2131	Abc transport albumen, permease protein, supposition	45/45	0/237	156，373

The immunogenicity of table 4. mouse epi-position

ORF	Amino acid from	Amino acid extremely	IB	Peptide ELISA	Seq ID
ORF	Amino acid from	Amino acid extremely	IB	Peptide ELISA	Seq ID	gbs0016	110	129	+		219
	168	184	+			gbs0016	110	129	+		219
	168	184	+			gbs0986	877	901	+		277
	333	354	+			gbs0986	877	901	+		277
	333	354	+				326	344	+
	801	809	+				326	344	+
	801	809	+			gbs1805	1	54		+++++	347
gbs2018	544	563	++	+++++	364	gbs1805	1	54		+++++	347
gbs2018	544	563	++	+++++	364		31	51	+	+++
	107	119	+				31	51	+	+++
	107	119	+				399	417	++	++++
	503	519	+	++++			399	417	++	++++
	503	519	+	++++		gbs0012	120	198	++	+	218
gbs0016	20	35	+	++	219	gbs0012	120	198	++	+	218
gbs0016	20	35	+	++	219	gbs0031	118	201		++++	221
gbs0428	48	132	+	+++++	242	gbs0031	118	201		++++	221
gbs0428	48	132	+	+++++	242	gbs0538	118	136	+	++++	249
gbs0580	162	178	+	+	253	gbs0538	118	136	+	++++	249
gbs0580	162	178	+	+	253	gbs0628	347	364	+	+++++	254
gbs0632	699	715	+	+++++	255	gbs0628	347	364	+	+++++	254
gbs0632	699	715	+	+++++	255	gbs0672	50	76		+	258
gbs0918	785	819	+	+++++	269	gbs0672	50	76		+	258
gbs0918	785	819	+	+++++	269		44	128	++
gbs0971	90	128		+++++	274		44	128	++
gbs0971	90	128		+++++	274	gbs1087	314	384	+		289
gbs1143	327	349		+++	293	gbs1087	314	384	+		289
gbs1143	327	349		+++	293	gbs1306	242	314	++	++++	304
	405	478	++			gbs1306	242	314	++	++++	304
	405	478	++				23	100	+
gbs1307	129	210	++		305		23	100	+
gbs1307	129	210	++		305	gbs1309	162	188		++	307
gbs1352	750	772	++	+++++	310	gbs1309	162	188		++	307
gbs1352	750	772	++	+++++	310	gbs1632	1	56		++	335
gbs1662	322	337	+	+++++	337	gbs1632	1	56		++	335
gbs1662	322	337	+	+++++	337	gbs1673	72	90	+	+++++	339
gbs1784	374	395		+	345	gbs1673	72	90	+	+++++	339
gbs1784	374	395		+	345	gbs1816	136	159	+	++++	348
gbs1899	141	164	+		358	gbs1816	136	159	+	++++	348
gbs1899	141	164	+		358	gbs1925	96	157	++	+	361
gbs2008	1	82		+	363	gbs1925	96	157	++	+	361
gbs2008	1	82		+	363	gbs2018	489	556	+	+++++	364
gbs2106	159	183		++	371	gbs2018	489	556	+	+++++	364
gbs2106	159	183		++	371		49	133	+	+++++

Table 5: the immunogenic protein that bacterium surface displaying is differentiated

The streptococcus agalactiae antigen protein	Infer function (passing through homology)	The immunogenicity amino acid * * that infers	Each ORF and the clone that selects of screening number	The position in the immunogenicity zone of differentiating (amino acid)	Seq. ID (DNA, protein)
The streptococcus agalactiae antigen protein	Infer function (passing through homology)	The immunogenicity amino acid * * that infers	Each ORF and the clone that selects of screening number		Seq. ID (DNA, protein)	gbs0233	Be similar to glycinebetaine/carnitine/choline abc transport albumen (osmotic protection is conjugated protein)	4-26，35-41，53-61，73-84， 103-108，114-120，140-146， 156-162，192-208，214-219， 227-233，239-252，260-268， 284-297	A：5，B：9， H：3	1-48， 113-133	463，475
gbs0419	Unknown function protein/lipoprotein	4-27，38-44，50-56，59-64，72-79， 83-89，92-97，108-116，123-148， 152-167，183-196，200-220， 232-244，255-261，265-274， 282-302，309-317	C：3，D：4， J：2，K：4	1-79， 231-302	464，476	gbs0233			A：5，B：9， H：3	1-48， 113-133	463，475
gbs0419	Unknown function protein/lipoprotein		C：3，D：4， J：2，K：4	1-79， 231-302	464，476	gbs0456	Cell walls surface anchoring family protein	6-28，66-72，85-105，115-121， 144-151，160-170，176-185， 223-230，252-288，296-310， 319-333，367-374，458-464， 471-480，483-488，520-528， 530-549，559-564，593-601， 606-616，636-643，655-662， 676-682，684-699，719-726， 735-750，757-764，777-785， 799-810，812-843，846-853， 868-873，880-889，891-899， 909-929，934-940，963-969， 998-1004，1007-1014，1016-1022， 1030-1046	A：9，B：125， F：9	1-80， 808-821	465，477
gbs0942	Unknown function albumen/lipoprotein	7-24，35-41，75-81，91-114， 122-132，137-144，148-156， 183-192，194-200，212-228， 233-238，251-258，275-295， 326-332，337-346	A：3，B：9， C：2，J：3	1-79， 305-321	466，478	gbs0456	Cell walls surface anchoring family protein		A：9，B：125， F：9	1-80， 808-821	465，477
gbs0942	Unknown function albumen/lipoprotein		A：3，B：9， C：2，J：3	1-79， 305-321	466，478	gbs0973	Gid protein (gid)	31-38，42-52，66-72，86-92， 98-104，115-122，127-146， 154-164，169-187，198-212， 225-237，255-269	D：2，G：3， H：8，I：2， K：2	13-92， 135-142	467，479
gbs0975	Agnoprotein matter	4-36，39-49，63-69，71-77，81-88， 123-131，133-139，160-169， 174-180，188-194，210-217， 273-278，289-300，317-334， 336-341，383-401，425-438	A：3，B：5， C：5，D：2， J：2	1-68， 212-270， 402-446	468，480	gbs0973	Gid protein (gid)		D：2，G：3， H：8，I：2， K：2	13-92， 135-142	467，479
gbs0975	Agnoprotein matter		A：3，B：5， C：5，D：2， J：2	1-68， 212-270， 402-446	468，480	gbs1038	Permease, supposition	21-29，31-42，49-63，72-79，81-93， 112-132，159-165，188-195， 197-232，262-267，279-286， 294-301，318-326，348-366， 381-405，409-426，436-465， 471-480，484-492，497-505，	B：2，C：3， E：3，J：2	1-57， 84-106	469，481

The streptococcus agalactiae antigen protein	Infer function (passing through homology)	The immunogenicity amino acid * * that infers	Each ORF and the clone that selects of screening number	The position in the immunogenicity zone of differentiating (amino acid)	Seq. ID (DNA, protein)
The streptococcus agalactiae antigen protein	Infer function (passing through homology)	The immunogenicity amino acid * * that infers	Each ORF and the clone that selects of screening number		Seq. ID (DNA, protein)			521-544，554-561，567-577， 581-589，601-609，611-622， 636-651，653-667，669-685， 700-708，716-722，729-744， 749-766，780-786，789-811， 814-864
gbs1144	Agnoprotein matter	6-24，35-48，57-63，72-78，87-92， 113-119，123-137，147-153， 173-181，212-233	C：30	1-124	470，482
gbs1144	Agnoprotein matter		C：30	1-124	470，482	gbs1279	Conserved domain albumen	13-34，62-69，78-83，86-91， 98-104，107-115，146-159， 179-188，195-205，209-221， 226-233，239-253，276-282， 284-294，297-308，331-354， 375-382，388-399，421-433， 449-458，464-469，472-491， 508-513，525-531，534-550， 575-593，601-618，629-635， 654-661，666-680，706-721， 723-740，771-805，810-830， 845-851	A：44，B：2， C：4	1-84	471，483
gbs1441	Conservative hypothetical protein matter	4-32，45-64，73-83，86-92， 100-111，125-147，157-163， 170-175，177-188，226-232， 245-252，258-274，320-335， 348-359	A：2，B：8， C：2	1-71	472，484	gbs1279	Conserved domain albumen		A：44，B：2， C：4	1-84	471，483
gbs1441	Conservative hypothetical protein matter		A：2，B：8， C：2	1-71	472，484	gbs1677	The sodium transport family protein	13-40，43-71，76-83，87-101， 109-119，125-156，162-175， 182-219，226-232，240-262， 270-287，306-318，326-342， 344-408，414-444，449-456	B：3，C：2， K：5	1-51	473，485
gbs2093	Membrane protein, supposition	4-16，18-34，45-54，99-108， 134-140，203-212，241-257， 266-274，279-291，308-315， 330-336，355-370，374-382， 402-410，428-455，466-472， 474-480，531-554，560-566， 572-580，597-618，632-660， 664-674，676-685，691-705， 708-735，750-768	B：5，C：8， D：2	1-87， 342-480	474，486	gbs1677	The sodium transport family protein		B：3，C：2， K：5	1-51	473，485

The immunoreactivity of epi-position and human serum in the antigen that table 6. is differentiated

Peptide	Positive	Amino acid from	Amino acid extremely	Sequence number
Peptide	Positive	Amino acid from	Amino acid extremely	Sequence number	gbs0233.1	+	115	132	475
gbs0233.2	+	1	26	475	gbs0233.1	+	115	132	475
gbs0233.2	+	1	26	475	gbs0419.1	++	33	55	476
gbs0942.2	+++	1	25	478	gbs0419.1	++	33	55	476
gbs0942.2	+++	1	25	478	gbs0973.1	+	37	61	479
gbs0975.1	+	1	24	480	gbs0973.1	+	37	61	479
gbs0975.1	+	1	24	480	gbs1038.1	+	1	23	481
gbs1144.3	++	46	60	482	gbs1038.1	+	1	23	481
gbs1144.3	++	46	60	482	gbs1279.1	+++	1	28	483
gbs1279.2	+	23	50	483	gbs1279.1	+++	1	28	483
gbs1279.2	+	23	50	483	gbs1279.3	++	45	71	483
gbs1441.1	+	1	22	484	gbs1279.3	++	45	71	483
gbs1441.1	+	1	22	484	gbs1441.2	++	17	38	484
gbs1677.1	+	1	22	485	gbs1441.2	++	17	38	484
gbs1677.1	+	1	22	485	gbs1677.2	+	17	38	485
gbs2093.1	+	1	27	486	gbs1677.2	+	17	38	485
gbs2093.1	+	1	27	486	gbs2093.2	++	22	47	486
gbs2093.4	+	422	447	486	gbs2093.2	++	22	47	486

The immunogenicity of table 7. mouse epi-position

ORF	Amino acid from	Amino acid extremely	Peptide ELISA	Sequence number
ORF	Amino acid from	Amino acid extremely	Peptide ELISA	Sequence number	gbs0233	115	132	++++	475
	1	47	++		gbs0233	115	132	++++	475
	1	47	++		gbs0419	1	55	++++	476
gbs0456	22	85	++	477	gbs0419	1	55	++++	476
gbs0456	22	85	++	477	gbs0942	307	320	+	478
	1	44	++		gbs0942	307	320	+	478
	1	44	++		gbs0973	15	76	++	479
	40	92	+++		gbs0973	15	76	++	479
	40	92	+++		gbs0975	1	59	++	480
	213	269	+		gbs0975	1	59	++	480
	213	269	+			403	445	+
gbs1038	1	56	++++	481		403	445	+
gbs1038	1	56	++++	481		85	105	+
gbs1144	37	121	+++++	482		85	105	+
gbs1144	37	121	+++++	482	gbs1279	1	71	+++++	483
gbs1441	1	38	+++	484	gbs1279	1	71	+++++	483
gbs1441	1	38	+++	484	gbs1677	1	38	+++	485
gbs2093	1	47	+++	486	gbs1677	1	38	+++	485

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Sequence table

SeqID 1

atgaagaaag ttttaacctt cttattatgt agcttatatt ttgtatcaat cccagctata 60

agtaccgagg agccattaac gctttctcaa aatagacgat acgccttaac tcagacagtt 120

gtggataagg aaatgtattt tgatgctatt ccagagagac caactacaaa aattgaaata 180

agcagttttc aagatgaagc tttaaccata acaggagaga ctttagttcc aaatacttta 240

ttgtcgattg tttctcttac aattaattca aatggaattc cagtatttac tttgtccaat 300

ggacagttca taaaggctag tcgtgaagct atttttaatg atttagtttc taagcagcaa 360

tctgtctcat tggattattg gttaaaacct tcttttgtga cttatgaagc accttacaca 420

aatggtgtta gtgaagttaa aaataattta aaaccttatt ccagagtgca tttagtagaa 480

caagcagaaa cagaacatgg tatttattat aaaacggaca gtggtttttg gatatcagta 540

gaagatctat cggttgctga taatcgtatg gctaaggttc aagaagtatt attggaaaaa 600

tataataaag ataaatatgg tatatacatc aaacaattaa atacacagac agtagctggt 660

attaatattg ataggtcaat gtactctgca agtattgcaa aattagcaac actttatgct 720

agccaagagc aagtaaaatt aggaaaactg tcattggaca gcaaatttga atacaaagat 780

aatgttaatc aatttcctaa tagctatgat ccaagtggta gtgggaaatt agagaaaaaa 840

gctgatcata aattatatac cgttaaggag ttacttgaag caactgccaa ggaatcagat 900

aatgttgcaa caaatatgct aggttattat gtcaataatc aatatgattc aatgtttcaa 960

acacaagtag acacaatttc tggtatgcac tgggatatga aaaaacgcca gatttctcca 1020

caagctgcgg gtaaaatgat ggaagcaatt tattatcaaa atggagatat tgttaattac 1080

ctatcaaaga ctgattttga taatacaaga atccctaaaa atataccagt taaagtagca 1140

cataaaattg gagatgctta tgactacaaa catgatgcgg ctattgttta tgcagaacaa 1200

ccttttatta tgattatctt tacggataaa tcatcttacg atgatatcac taagattgct 1260

gacgacgttt accaggtt ttaaaataa 1287

SeqID 2

atgaaaaaaa gaatattatc agcagtcctt gtgagtggag taactcttgg aactgctgcc 60

gtaacagtaa acgctgatga ctttgactcg aaaattgctg ctactgattc agtaatcaat 120

actttatcag ggcaacaagc agcagcacaa aaccaggtga ctgctattaa aggacaagtt 180

ggtgctttag agtctcaaca atcagaatta gaagcgcaaa atgctcaact tgaggctgta 240

tctcagcagt taggtcaaga gattcaaact ctttctaata aaattgttgc acgtaacgaa 300

tctttgaaaa agcaagttcg tagtgctcaa aagggtaact tgactaacta tattaatact 360

attttaaatt cgaaatcagt atctgatgct gttaatcgtg ttgttgctat tcgtgaggtt 420

gtttcagcta atgaaaagat gttagcacaa caagaggctg acaaagcggc cttagaagct 480

aaacaaattg aaaatcaaaa tgctataaat actgtagcag ctaacaagca agctatcgaa 540

aataataaag cagccttagc aacacaaaga gcacaattag aagcagctca attagagtta 600

tctgctcaat tgactacagt ccaaaatgaa aaagcatctt taattcaggc aaaagcacaa 660

gctgaggaag ctgcgcgtaa ggcagctgaa gcacaagcag cagctgaagc gaaagcacaa 720

gctgaagcga aagcacaagc tgagtctgta gcaaaagcac aggcagcagc acaagttgag 780

tctgccacag ctcctactga aacagttcaa acacaaccga gaactgaaat aaagccttct 840

aatcttacgg ctacttcttc ggcaactact gttgctacaa ctacagctac agctactaat 900

gagccaaaag ttactcaacc ttctgtagta actaaagcag ttgaagcacc taaagcagtt 960

gtttcttcaa cacctagagc ggtatcaaaa cctgttgtca gaagttatga ttcaagtaac 1020

acatacccaa tgggacaatg tacttgggga gctaagtcaa tggcttcttg ggttggtaac 1080

tattggggaa atgctaatca gtggggtgct agtgcacgtg cagctggtta ttctgtagga 1140

actacccctc gtgttggtgc agtagctgtt tggccatatg atggtggtgg ttacgggcac 1200

gttgcagtag taacgtcagt ggctaacaat tcatctatcc aagttatgga atcaaactat 1260

gctggaaata tgagtattgg caattaccgt ggttcattta accctagtgc ttctggctct 1320

gtttactata tttacccaaa ttaa 1344

SeqID 3

atgaataaac gtatttttgt tgagaaaaag gctgactttg acattaaatc ggctagtctt 60

gtgaaagagt tgacgcataa tctacaactg acttctttga aggatttgcg tattgtgcag 120

gtctatgatg tcttcaattt ggctgaggat ttgctggcgc gtgctgagaa gcatattttc 180

tctgagcagg tgacagactg tcttttgacg gaaactgaaa tcactgcgga gcttgataag 240

gttgccttct ttgccattga ggcgcttcct ggtcaatttg accaacgtgc tgctagttcg 300

caagaatctt tgctattgct tggaagtgac agtcaggtta aggtcaatac agcccagctt 360

tacttggtca ataaggatat tgcagaagca gagcttgaag ccgttaagaa ctatcttttg 420

aatcctgttg attcacgttt caaggacatt accttgccgc ttgaagagca ggctttctct 480

gtgtatgata agacaatccc tagtcttgat ttctttgaaa cttataaggc tgacgatttt 540

gcggcctata aggcagagca gggcttggct atggaggtcg atgatcttct cttcatccaa 600

gattatttca aatcaatcgg gcgtgtgcca actgagactg agttgaaagt tttggatact 660

tactggtcag accactgccg tcacacaacc tttgaaactg agttgaaaaa catcgacttt 720

tcagcttcta aattccaaaa acaattgcag gcgacttatg acaaatatat cgccatgcgt 780

gatgaacttg gtcgttctga aaagccgcag acacttatgg atatggcgac tatctttggt 840

cgttatgaac gtgccaatgg acgactggat gatatggaag tctcagatga aatcaatgcc 900

tgctcagttg aaattgaagt agatgttgat ggtgtaaaag agccttggct cctcatgttt 960

aagaatgaga ctcacaatca cccaacagaa attgagccat ttggtggcgc agcaacttgt 1020

atcggtggtg ccattcgtga cccgttgtca ggacgttcat acgtttatca ggctatgcgt 1080

atttcaggcg caggcgatat tacgactccg attgcggaaa cacgtgctgg taaattgccg 1140

caacaggtta tttctaaaac tgcggcgcac ggctattctt catatggtaa ccagattggg 1200

cttgcgacaa cttatgtgcg tgagtacttt caccctggct ttgtagccaa acgcatggag 1260

cttggagctg tggttggtgc tgcacctaag gaaaatgtgg ttcgtgaaaa accagaagca 1320

ggcgatgtgg tcatcttgct cggtggtaaa acaggtcgtg acggtgtcgg cggtgcgaca 1380

ggttcatcta aggttcaaac ggttgaatct gtggaaacag ctggcgcaga ggtacaaaaa 1440

gggaatgcca tcgaagaacg taagattcaa cgccttttcc gtaatggtaa tgtcactcgc 1500

ctaatcaaga aatcaaatga cttcggtgca ggtggtgtct gtgttgccat cggtgaattg 1560

gctgacggtc ttgaaatcga tttggacaag gtaccgctta aataccaagg tcttaatggt 1620

actgaaattg caatctcaga atcacaagag cgtatgtcag tcgttgttcg tcctagtgac 1680

gtggatacct tcatcgcagc ctgcaacaag gaaaatatcg atgccgttgt ggttgctact 1740

attactgcaa aaccaaacct tgtcatgact tgggatggtg aaacaattgt tgacttggaa 1800

cgccgtttcc ttgataccaa cggtgtccgt gtcgttgttg atgctaaagt cgttgacaaa 1860

gacttgacag ttccagaggt acgcacaaca tcagcagaga cacttgaagc ggatacgctt 1920

aaggtcttgt ctgacctcaa ccacgctagt caaaaaggtc tccaaactat ctttgactca 1980

tctgttggtc gctcaaccgt taaccaccca atcggtggcc gttaccaaat cacaccaaca 2040

gaaagctctg ttcaaaaatt gccagttcaa cacggtgtca caacaactgc atctgttatg 2100

gctcaaggtt acaatcctta tattgcagaa tggtcacctt accacggtgc tgcctatgct 2160

gtcattgaag cgacagctcg cttggtagca acgggtgctg actggtctcg tgcacgtttc 2220

tcttaccaag agtatttcga gcgtatggat aaacaggcag agcgttttgg tcagccagta 2280

tcagctcttc ttggttctat tgaggctcag attcaacttg gattgccatc aatcggcggt 2340

aaggactcta tgtctggtac tttcgaagaa ttgacagtac cgccaacctt ggtagctttc 2400

ggtgtgacaa cagcggacag ccgcaaggtt ctctctcctg agtttaaagc ggctggtgag 2460

aatatctact acattccagg tcaagctatt tcagaagata ttgattttga ccttatcaag 2520

gctaacttta gccagttcga gactattcaa gctcaacata agattactgc tgcttcagct 2580

gttaaatacg gtggtgtcct agaaagtctt gctatcatga cttttggtaa ccgtatcggg 2640

gcctctgttg aaattgcaga gcttgacagtagcttgacag ctcaactcgg aggctttgtc 2700

tttacatcag ctgaggaaat tgctgactcg gtgaaaatcg gtcaaactca ggcagccttt 2760

acacttactg tcaatggaaa tgaccttgct ggggcaagcc ttctatcagt cttcgaaggc 2820

aaattggaag aggtttaccc aacagagttt gaacaggcag acgctcttga agaagttcct 2880

gctgtggtat cagatactgt tatcaaggct aaggaaacaa ttgaaaaacc agtggtttac 2940

attccagtct tccctggtac caactcagaa tatgattcag ctaaggcctt tgaacaggtt 3000

ggagctagtg tcaacttggt agcatttgta accttgaatg aggctgctat tgctgactca 3060

gttgacacaa tggtcgctaa tattgctaag gcaaatatca tcttctttgc tggaggattc 3120

tcagcagcgg atgaaccaga tgggtctgct aagtttatcg tcaatatctt gcttaacaag 3180

aaggttcgcg cagctattga cagcttcatc gaaaaaggtg gccttatcat cggtatctgt 3240

aatggtttcc aagcccttgt taaatcaggt cttcttccat acggaaactt cgaggaagct 3300

ggtgagacaa gtccaaccct cttctataac gatgccaacc agcacgttgc caagatggtt 3360

gagactcgta tcgcaaatac caactcacct tggttggtag gagttgaggt cggcgatatt 3420

catgccatcc cagtttcaca cggtgaaggt aaatttgttg tcagcgcttc tgaatttgca 3480

gagctaagag acaatggtca aatctggagc caatatgtgg actttgacgg acaaccatct 3540

atggattcta aatacaatcc aaatggctct gtcaatgcca tcgaagggat taccagcaag 3600

aatggtcaaa tcatcggtaa gatggggcac tcagaacgct gggaagacgg cctcttccaa 3660

aatatccctg gtaacaaaga ccaagccttg tttgctagtg cagtaaaata ttttacagga 3720

aaataa 3726

SeqID 4

atgaaaatga ataaaaaggt actattgaca tcgacaatgg cagcttcgct attatcagtc 60

gcaagtgttc aagcacaaga aacagatacg acgtggacag cacgtactgt ttcagaggta 120

aaggctgatt tggtaaagca agacaataaa tcatcatata ctgtgaaata tggtgataca 180

ctaagcgtta tttcagaagc aatgtcaatt gatatgaatg tcttagcaaa aataaataac 240

attgcagata tcaatcttat ttatcctgag acaacactga cagtaactta cgatcagaag 300

agtcatactg ccacttcaat gaaaatagaa acaccagcaa caaatgctgc tggtcaaaca 360

acagctactg tggatttgaa aaccaatcaa gtttctgttg cagaccaaaa agtttctctc 420

aatacaattt cggaaggtat gacaccagaa gcagcaacaa cgattgtttc gccaatgaag 480

acatattctt ctgcgccagc tttgaaatca aaagaagtat tagcacaaga gcaagctgtt 540

agtcaagcag cagctaatga acaggtatca ccagctcctg tgaagtcgat tacttcagaa 600

gttccagcag ctaaagagga agttaaacca actcagacgt cagtcagtca gtcaacaaca 660

gtatcaccag cttctgttgc cgctgaaaca ccagctccag tagctaaagt agcaccggta 720

agaactgtag cagcccctag agtggcaagt gttaaagtag tcactcctaa agtagaaact 780

ggtgcatcac cagagcatgt atcagctcca gcagttcctg tgactacgac ttcaccagct 840

acagacagta agttacaagc gactgaagtt aagagcgttc cggtagcaca aaaagctcca 900

acagcaacac cggtagcaca accagcttca acaacaaatg cagtagctgc acatcctgaa 960

aatgcagggc tccaacctca tgttgcagct tataaagaaa aagtagcgtc aacttatgga 1020

gttaatgaat tcagtacata ccgtgcggga gatccaggtg atcatggtaa aggtttagca 1080

gttgacttta ttgtaggtac taatcaagca cttggtaata aagttgcaca gtactctaca 1140

caaaatatgg cagcaaataa catttcatat gttatctggc aacaaaagtt ttactcaaat 1200

acaaacagta tttatggacc tgctaatact tggaatgcaa tgccagatcg tggtggcgtt 1260

actgccaacc actatgacca cgttcacgta tcatttaaca aataa 1305

SeqID 5

atgttaaaac attttggtag taaagtaaga aatttgagag taactagaaa tattactcgc 60

gaagattttt gtggtgatga aactgagtta tctgtaagac aattagctag gattgaaagt 120

ggacagtcaa ttccaaactt gactaaagct cactacatag ctaaacaatt gaatgtaaaa 180

ttggatattt taacaggagg agagagctta gaattaccta aacggtataa agagttgaag 240

tacaaattgt tgcgtacccc gacttacgga gatgctaata gacttgcagt tagagaagca 300

tattttgatg aaatctatga agtattttat gaggagctcc cagaagatga aagactaatt 360

attgattgta tgcaatcaaa attagatgtt catttttctg ttaatgataa ttttggaatc 420

actattttac atgactactt tgatcaaatt aagaaaaaaa aggaatatac cacaaatgat 480

tttgttatga tagatttata tttactttgc ttttctatta actatggaat gaagagcttg 540

tattctttag agaactatca ttttattatg tcaaaattgt tagagcaaga taatttactt 600

cctgaggata attttcaatt gaacaacgtt ttattaaatc atgtcgaatt agcttttcaa 660

tttaagcaga aaaaatatgt acagcaaatt attcatagaa gtaatgctat tatgacagaa 720

atccatgatt ttcaaaaaag accaatacta agcttaatag agtggaaata tttattaatt 780

attgaaaaag atagaacaaa agcagaaaca tgttttaaac aatcaattct atttgcagag 840

ttaataggtg atctatattt aaaaggaaaa ttaatagagg agtggaataa agatttaaca 900

tga 903

SeqID 6

atgactgaaa aaactaaagc ggtagaaact acagatgttg ctcttgcaat tgatacattg 60

gttcaaaacg gtttaaaagc acttgatgag atgcgtcaac ttaatcaaga acaagttgat 120

tatattgtag ctaaagcttc tgttgcagcc ttggatgccc atggagaact tgctcttcat 180

gcagtagaag aaactggtcg tggtgtcttt gaagataaag cgacaaaaaa tttatttgca 240

tgtgaacacg tcgttaacaa catgcgtcat acaaagacag tgggagttat tgaagaagac 300

gatgtcacag gcttgacatt aattgctgaa ccagtgggag ttgtctgtgg tattacgcca 360

acaaccaacc cgacttcaac tgctattttc aagtcattaa tctctcttaa aacacgtaat 420

ccaattattt ttgccttcca cccatcagca caagaatcat cagcacatgc agcacgtatt 480

gttcgtgatg ctgctattgc tgctggagct cctgaaaatt gtgtccaatg gattgaacaa 540

ccatcgattg atgcaactaa tgctttgatg aaccatgatg gtattgccac tatccttgcg 600

acaggtggta atgcgatggt taaagctgcc tactcatgtg ggaaacctgc ccttggtgta 660

ggtgctggga atgttccagc ttatgttgaa aaatcagcta atatccgaca agccgctcac 720

gatattgtta tgtctaaatc atttgataac ggtatggtat gtgcatcaga acaggctgtt 780

attattgata aagaaattta taaagaattt gttgaagaat ttaaatctta tcatacttat 840

ttcgttaata agaaagaaaa agctcttctt gaagaattct gttttggtgc aaaagcaaat 900

agcaaaaact gtgctggtgc aaaattaaat ccaaatattg ttggaaaatc agcagtttgg 960

attgctgaac aagctggatt tactgttcca gaaggaacaa atattcttgc cgcagaatgt 1020

actgaagtta gtgaaaaaga acctttgaca cgtgaaaaac tatcacctgt tattgccgta 1080

ttgaaagctg aatctacaga agatggtgtt gaaaaagctc gtcaaatggt tgagtttaat 1140

ggtctaggac actcagctgc tattcataca aaagatgcag acttagctcg tgaatttgga 1200

actagaattc gtgctatccg tgttatttgg aattctcctt ctacttttgg tggtattggg 1260

gatgtttaca atgctttctt gccatcattg actcttggtt gtggttcgta tggacgtaac 1320

tcagttggtg ataacgttag tgctataaat ctcttgaata tcaaaaaagt aggaagacgt 1380

agaaataata tgcaatggtt taaagtccct tcaaaaacat acttcgaacg tgattctatt 1440

caatatttgc aaaaatgtcg cgatgttgaa cgtgtcatga ttgttacaga tcacgccatg 1500

gttgagcttg gtttcttgga tcgtatcatt gaacaacttg atcttcgtcg caataaagtt 1560

gtttatcaga tttttgctga agtagaacca gatccagaca ttacaacagt aatgaaagga 1620

acagatttga tgcgtacctt caaaccagac actattattg cattaggtgg tggttctcct 1680

atggatgctg cgaaagttat gtggctcttc tatgaacaac cagaagttga tttccatgac 1740

ctcgttcaaa aattcatgga tatccgtaaa cgtgcgttca aattcccaga acttggtaag 1800

aaaacaaaat ttgttgctat tccaacaact tctggaacag gttctgaagt gacaccattt 1860

gccgttatct ctgacaaagc aaataatcgt aaatatccaa tcgctgatta ttcattaaca 1920

ccaactgtag ctatcgttga tccggccctt gttatgactg ttcctggttt tattgcggct 1980

gatacgggta tggatgtatt gacacatgca acagaggctt atgtttcaca gatggcaaat 2040

gattatacag acggacttgc ccttcaagca attaaaattg tgtttgacta cttagaacgc 2100

tcagtaaaag atgctgattt tgaagcacgt gaaaagatgc acaatgcgtc aactatggca 2160

ggtatggctt tcgccaatgc attcttaggt atttctcact caatggcgca taaaattggc 2220

gcacaattcc ataccgtaca cggacgtaca aatgcaattc ttttgccgta tgttatccgc 2280

tataatggaa ctcgtccagc taaaacagca acatggccta agtacaatta ctatcgtgct 2340

gatgaaaaat atcaagatat cgctaaatta ctcggcttac cagcagcaac accagaagaa 2400

gctgtcgaat catatgcaaa agctgtgtat gaccttggaa cacgtttagg tattaagatg 2460

aatttccgcg accaaggcat tgatgaaaaa gaatggaaag aaaaatctcg tgaactagct 2520

ttcttagcat atgaagatca atgttcacca gcaaatccac gccttccgat ggtagatcac 2580

atgcaagaaa tcatcgaaga tgcttattat ggttatgagg aacgtccagg acgtcgtaag 2640

taa 2643

SeqID 7

gtgggtatta aagtttataa accaacgaca aatggccgtc gtaatatgac ttctttggat 60

tttgcagaaa tcactacaaa cactcctgag aaatcattgc ttgtttcact aaagaataaa 120

gcaggacgta acaacaacgg acgtattact gttcgtcacc aaggtggtgg acacaaacgt 180

cattaccgtt tgattgactt caaacgtaat aaagatggtg ttgaagcagt tgttaaaaca 240

attgaatacg atccaaatcg tactgcaaat attgctcttg tacactatac tgatggtgtt 300

aaagcttaca ttcttgcgcc taaaggcctt gaagtaggtc aacgcattat ttctggtcca 360

gaagcagata tcaaagttgg taacgcactt ccacttgcta acattccagt cggtacagtt 420

atccataata ttgaattgca accaggtaaa ggtgctgaat tgattcgtgc cgcgggtgct 480

tctgctcaag tacttggtca agaaggtaaa tatgttcttg ttcgccttca atcaggtgaa 540

gtacgtatga tccttggtac ttgccgtgca acaattggta ctgttggtaa tgaacaacaa 600

tcacttgtta acattggtaa agcaggacgt aatcgttgga aaggtgttcg ccctacagtt 660

cgtggttctg taatgaaccc taatgatcac ccacacggtg gtggtgaagg taaagcacca 720

gttggtcgta aagcaccatc tactccatgg ggtaaacctg cacttggact taaaactcgt 780

aataagaaagctaaatcaga caaacttatt gttcgtcgtc gtaaccaaaa ataa 834

SeqID 8

atgattgagt ttgaaaaacc aataataaca aaaattgatg aaaataaaga ttacggcaga 60

tttgtcatcg aaccactaga acgtggctac ggtacaactc taggaaactc tcttcgtcgt 120

gtgcttctgt cttcacttcc aggtgcagca gtcacatcaa tcaaaattga tggagtatta 180

cacgaatttg atactatccc aggtgtacgt gaagatgtga tgcaaatcat ccttaatgta 240

aaaggacttg ctgtgaaatc ttacgtcgaa gacgaaaaga ttatcgaact agacgttgaa 300

ggtcctgcag aaattactgc cggagatatt ttaacagata gtgatatcga aattgtaaac 360

cctgaccatt atcttttcac aattgctgaa ggtcacagtt tgaaagcaac aatgacagtt 420

gctaaaaacc gaggttatgt tccagcagaa ggaaataaaa aagatgatgc accagtggga 480

acattggctg tagattcaat ctatacacca gtgaaaaaag ttaattatca agttgaacct 540

gctcgtgtag gtagtaacga tggctttgat aaattaacta ttgaaatcat gacaaatggc 600

acaatcattc ctgaggatgc tcttggtctt tcagctcgcg tcttaattga acacttaaac 660

ttgtttactg atttaacaga ggttgctaaa gcaactgaag taatgaagga aacagagaaa 720

gtgaacgatg agaaagtgct tgaccgcaca attgaggaac ttgatttgtc agtgcgctca 780

tataactgtt taaaacgtgc tggaattaat acagtatttg atttaacaga aaaaactgag 840

cctgaaatga tgaaagtccg aaacttagga cgtaagagtc ttgaagaagt taaaatcaaa 900

cttgccgatc taggtcttgg attaaaaaac gataaataa 939

geqID 9

atgaaaaaga ttcgattatc aaagtttatt aaaatgattg ttgttatttt gtttttaatt 60

agtgtagcag ctagttttta ttttttccac gttgcccaag ttcgagatga taaatccttt 120

atttcaaatg gtcaacgtaa gcctggaaac tctttatatg cttatgataa atcctttgat 180

aagctattaa agcaaaaaat agaaatgaca aaccaaaata taaagcaagt tgcttggtat 240

gttcctgctg ctaagaaaac tcataagaca gctgttgtcg ttcatggttt tgcgaatagc 300

aaagagaata tgaaggcata tggttggctg tttcataagt taggatacaa tgttcttatg 360

cctgacaata ttgcacatgg tgaaagtcat gggcagttga taggctatgg ctggaacgac 420

cgcgagaaca ttatcaaatg gacagaaatg atagttgata agaatccatc aagccaaatt 480

actttatttg gtgtttcaat gggtggagca acagtcatga tggctagtgg tgaaaaatta 540

cctagtcagg ttgttaatat cattgaagat tgcggttatt ctagtgtttg ggatgaatta 600

aaatttcagg ctaaagagat gtatggttta ccagccttcc cactcttata tgaagtttca 660

acaatttcta aaatcagagc aggtttttcg tatggacaag caagtagtgt cgaacaattg 720

aaaaagaata atttaccagc cctctttatt catggtgata aggataattt tgttccaaca 780

agtatggttt atgacaacta taaagctaca gcaggtaaga aagagcttta tattgtaaaa 840

ggggcaaaac atgcgaaatc ttttgaaaca gagccagaaa aatatgagaa acgtatctct 900

agttttttga aaaaatatga aaaataa 927

SeqID 10

atgtcaaaagtacgtggctt tgaattagtt agccaattta gcaataaaga gttattacca 60

aaacgagaaa cagctcacgc agctggctat gatttaaaag tagctaaaaa aacggttatt 120

gaacctggtg aaattacatt ggtcccgacc ggtattaaag cacacatgca accaggggag 180

gtcctgtacc tttatgatcg ttcatcgaat ccacgtaaaa aagggattgt cctaataaat 240

tcagttggtg ttattgatgg tgactactat aataaccaag tcaatgaagg acatattttt 300

gcacagatgc aaaatattac tgatcaggct gttattttag aagaagggga acggattgtt 360

caggctgttt ttgccccatt cttattggca gatgacgatc aggctacagg aatgcgtaca 420

ggtggttttg gtagtactgg aaaataa 447

SeqID 11

atgaaatttg gaaaaaaact tggctttcta gccttattga tgtctattgt cctcattcta 60

ggtgcatgtg gtaaaactgg acttggaaat tctactggga attctacaaa aaatgtaacc 120

aaaaaatctg ctaaaaactt gaaactaggt gtatctattt caacaactaa caatccctac 180

tttgttgcga tgaaagacgg tattgacaaa tatgctagca ataaaaaaat aagcattaag 240

gtagctgatg ctcaagatga cgccgcacgt caagcagatg atgtccaaaa ctttattagt 300

caaaacgtag atgctatttt gattaatccc gttgactcaa aagctattgt aacagctatt 360

aagtctgcaa acaacgctaa tattcctgtt attctaatgg accgtggtag tgagggaggc 420

aaagtattaa ctactgtcgc ttcagataac gttgctgctg gtaagatggc tgcggattat 480

gctgttaaaa aactaggcaa aaaagcaaaa gcctttgaat tatctggagt accaggtgcc 540

tctgctactg tagaccgtgg taaaggattt cattcagttg caaaatctaa acttgacatt 600

ctttcaagcc aatctgccaa ttttgaccgc gccaaagctt tgaatactac acaaaatatg 660

attcaaggac ataaagatgt ccaaattatt ttcgcacaaa atgatgaaat ggcacttggg 720

gctgcacagg ctgtcaaatc tgctggcttg caaaatgttc ttatcgtagg aattgatggt 780

cagccagatg cccatgatgc cattaaaaaa ggagatattt cagccactat tgcacaacaa 840

ccagctaaga tgggagaaat agcaattcaa gcagcaattg atcattataa aggcaaaaaa 900

gtagagaaag aaacaatttc tccaatttac cttgttacca aagacaatgt tgaaaaatac 960

aattggtaa 969

SeqID 12

atgggaaaag aaaaattaat tttagcttat tctggtggtt tagatacctc tgttgctata 60

gcttggttaa aaaaagatta tgacgttatt gctgtctgta tggatgttgg cgaaggtaaa 120

gatcttgatt ttattcacga taaggcttta acaataggtg ccatagaatc ttatatttta 180

gatgttaaag atgagttcgc tgagcatttt gtgttaccag ctttacaggc acatgcgatg 240

tatgaacaaa aatacccgct tgtttcagcg cttagtcgtc caattattgc tcaaaaatta 300

gttgaaatgg cacatcaaac aggtgcgacc acaattgccc atggatgtac aggtaaagga 360

aatgaccaag tgcgttttga agttgcaatt gcagcactag atcctgaatt aaaagtaatt 420

gcccctgttc gtgagtggaa gtggcatcgt gaagaggaaa ttacttttgc aaaagctaac 480

ggcgtaccta ttccagcaga tttagataac ccttattcta tagaccaaaa tttatgggga 540

cgtgccaatg aatgtggtgt gcttgagaat ccttggaatc aagcgccaga agaagctttt 600

ggtattacaa aatcaccaga agaagctcct gattgtgcag aatatattga tattacgttc 660

caaaatggga agcctatagc tataaacaat caagaaatga cactagcaga tttgatttta 720

tcgctaaatg aaattgcagg aaaacatggt attggacgca ttgatcatgt tgaaaatcgt 780

ttagtcggta tcaaatcacg tgaaatttat gaatgtcctg cggcaatggt actgttagct 840

gctcataaag aaattgaaga tttaacatta gtccgtgaag tgtctcattt taaacctatc 900

cttgaaaatg aactatcaaa tcttatttat aatgctttat ggtttagtcc agctacaaaa 960

gctatcattg cctatgtcaa agaaacacaa aaagtggtta atggtactac aaaagttaag 1020

ttatataaag gttctgccaa agtcgttgct cgccactcgt ctaattcatt atatgatgag 1080

aacttagcaa cttatacagc tgctgataac tttgatcaag atgcagcagt tggttttatc 1140

aaactctggg gacttccaac ccaagttaat gcacaagtaa acaaaggata a 1191

SeqID 13

atggctaaag tatgttattt cacaggacgt aaaacagttt ctggtaacaa ccgttcacac 60

gcgatgaacc aaacaaaacg tactgttaaa ccaaaccttc aaaaagttac tgttcttatc 120

gacggtaaac caaaaaaagt ttgggtttca gctcgtgcgc ttaaatctgg taaagtagaa 180

cgcgtttaa 189

SeqID 14

atggcaaaac agaaaaataa ctggcgccgt gttggagttg gtgtccttac acttgcttca 60

gttgcgactc ttgctgcatg tggaagtaaa tcagcttccc aggattctaa tggagcgatt 120

aattgggcta ttccaacaga aatcaataca ctagatttat ctaaagttac agacacttac 180

tcaaatctag ctattggtaa ctctagtagt aatttccttc gcttagataa ggatggaaag 240

acgagaccag atttggctac taaagttgat gtttcaaaag atggtttaac ttatacagct 300

acattacgta aaggcttgaa gtggtcagat ggcagtaaac ttactgcaaa ggattttgtt 360

tattcatggc aacgtttagt tgatcctaag acagcttcac aatatgctta ccttgctgtt 420

gaagggcatg tgcttaatgc cgataaaatc aacgaaggac aagagaaaga cttgaataag 480

ctaggtgtta aggcagaagg cgatgacaaa gttgttatta ctttatctag tccgtctcca 540

caattcatct actaccttgc attcactaat ttcatgccac aaaaacaaga agttgttgaa 600

aaatatggaa aagattacgc aactacttca aaaaatacag tttactcagg accatatact 660

gttgaaggtt ggaatggttc aaatggtact ttcaccctga agaaaaacaa aaattattgg 720

gacgctaaaa atgtaaaaac aaaagaagtt cgcatccaga ctgttaaaaa accagatacc 780

gccgttcaaa tgtataaacg tggtgaatta gatgcagcta atatctcaaa tacttctgct 840

atttatcaag ctaataaaaa taataaagat gtcacagatg ttctagaagc gaccactgcc 900

tatatgcaat ataatactac tggttctgtg aaagggcttg ataatgttaa gattcgtcgc 960

gccttaaact tagcaactaa ccgtaaagga gttgttcaag cagccgttga tacaggctca 1020

aaaccggcaa ttgcttttgc accgactggt ttagccaaaa caccagatgg aactgatttg 1080

gcaaaatatg ttgccccagg ttatgaatat aataaaactg aagcagcaaa actctttaaa 1140

gaaggtttgg ctgaatcagg cttgactaag ctaaaattaa caattacagc agatgctgat 1200

gttcctgctg ccaaaaactc tgttgactat atcaagtcta cttgggaagc tgctcttcca 1260

ggacttactg ttgaagaaaa atttgtaacc tttaaacaac gcttagaaga cagtagaaaa 1320

caaaactttg atatcgtagt ttctgtttgg ggtggggatt atccagaagg gtcaactttc 1380

tacggcctct ttaagtcaga ttcacaaaat aacgatggaa aatttgctaa caaggactat 1440

gacgctgctt ataacaaggc aatttccgaa gatgccttaa aaccagaaga atcagcaaag 1500

gactataaag aagcagagaa aattctattt gagcaaggcg cttataaccc actctacttc 1560

cgtagtggta aaggtttaca aaatccaaaa ttaaaaggtg ttattcgtaa tactacaggt 1620

ttgtcaatag actttacaca tgcttataaa aaataa 1656

SeqID 15

atggaactct taaaaacacc catctttggt atttgctttt ctttaatact ctatacgata 60

ggacaacatt tatttaagaa gagtaaaggt ttcttccttt tgcagcctct tttctttgca 120

atggttagtg gtattgtcat tctttggctt atgtcaaaag gtttaggaac cgatgttaag 180

acattttata cacaagctta taaaccaggt ggcgatttaa tattttggtt tttaaatcca 240

gcaacaattg cttttgcagt tcctctctat aagaaaaatg acgttgttaa aaaatattgg 300

gtagaaattc tcagcagttt agtaatcggt atgattgttt cccttatgct tatcgtcgct 360

atttctaaaa tggttgggct tagtcaagtc ggaattgctt caatgttgcc acaagcagca 420

acaacagcaa ttgctcttcc aataacagca gcaattggag ggaacacagc tgtgacagca 480

atggcgtgta tcttaaatgc agttattatt tatgcattag gtaaaaaatt agtgtcattt 540

ttccatttga atgatagtaa gattggtgca ggattaggtc tagggacctc tggtcataca 600

gtcggagcag cctttgcatt ggaattggga gaactgcaag gtgcgatggc agctatagcg 660

gtggtggtta tcggtttggt agttgatttg gttattccta tctttagtca tttgattggt 720

ttgctataa 729

SeqID 16

gtgactaaat atttgaagta catctctttt gttgctttgt ttctggcgag tatcttttta 60

gtagcttgtc aaaatcaaaa ttcacaaaca aaggagcgaa cacgaaaaca acgacccaaa 120

gatgaattgg ttgtttctat gggggcaaag cttcctcatg aattcgatcc aaaggaccgt 180

tatggaatcc ataatgaagg taatattact catagtaccc tattgaaacg ttctcctgaa 240

ctagatataa aaggagagct tgctaaaaaa tataaaatct ctaaggatgg cttaacgtgg 300

tcgttcgact taaatgatga ttttaaattc tccaatggtg agcctgttac tgctgacgat 360

gttaagttta cttatgatat gttgaaagca gatggaaaag cttgggattt gacctttatt 420

aagaatgttg aagtagttgg gaaaaaccag gtaaatattc atttgactga ggcgcattcg 480

acatttacag cacagttgac agaaatccca atcgtcccta aaaaacatta caatgataag 540

tataagagca atcctatcgg ttcaggacct tacatggtaa aagaatataa ggctggagaa 600

caagcaatct tcgttcgaaa tccatattgg cacggtaaaa agccttattt taaaaagtgg 660

acttgggtat tacttgatga aaatacagca ctagctgctt tagaatctgg tgatgttgac 720

atgatttacg caacgccaga gcttgctagc aagaaagtca aaggaacacg tcttctagat 780

attgcttcaa atgatgttcg tgggttatcg ctaccttatg tgaagaaagg tgttgtgaaa 840

aattcaccag acggttatcc agtaggaaat gatgtcacta gtgatccagc aatcagaaaa 900

gccttgacta ttggtttaaa tagacaaaaa gttctggata ctgttttaaa tggttatggt 960

aaaccagctt attcaattat tgatagaaca ccattctgga atccaaaaac agcaattaaa 1020

gataataaag tagctaaagc taagcaactt ttgacaaaag ctggatggaa agaacaagca 1080

gacggtagcc gcaaaaaagg aaatcttaaa gcggaatttg acctttacta ccctactaat 1140

gatcaattac gagcaaactt agccgttgaa gtagcggagc aagctaaagc cttaggtatt 1200

actattaaac tcaaggctag taactgggat gaaatggcaa ctaagtcaca tgattcagcc 1260

ttactttatg cgggaggacg tcatcatgcg cagcaatttt atgaatcaca ttacccaagt 1320

ttagctggta aaggttggac caatattact ttttataaca atcctactgt gactaagtac 1380

cttgacaaag caatgacatc tcccgacctt gataaagcta acaaatattg gaagttagct 1440

cagtgggatg gcaaaacagg tgcttccact cttggagatt taccaaatgt atggttggtg 1500

agtcttaacc atacttatat tggtgataaa cgtatcaatg taggtaaaca aggtgtccat 1560

agtcatggtc atgattggtc attattgact aacattgctg aatggacttg ggatgaatct 1620

gctaagtaa 1629

SeqID 17

atgggccaag aacctatcat cgaatatcaa aatatcaata aagtgtatgg ggaaaatgtt 60

gcggttgaag atattaacct taaaatttac cctggtgatt tcgtttgttt catcggtacg 120

agtggatcag gtaaaacaac attaatgcgt atggttaacc atatgttaaa accaacaaat 180

ggtactctat tatttaaggg aaaagatatt tctactatta accccattga attaagacgc 240

agaattggat atgttatcca aaacattggt ttaatgcctc atatgaccat ttacgaaaat 300

atagttcttg taccaaaatt attgaaatgg tcagaagaag ctaaaagagc taaagcaagg 360

gaacttatta aattagttga attacccgaa gaatatttgg atcgctaccc tagtgagttg 420

tctggcggtc agcaacaacg tatcggtgtc attcgcgctc ttgcagcaga ccaagatatt 480

attttaatgg atgagccttt tggagctctg gatcctatta ctagagaagg tattcaagac 540

ttagtcaagt ctcttcagga agaaatgggg aaaactatca tcttagttac tcatgatatg 600

gatgaagccc tcaagttagc aacaaaaatt attgttatgg acaatggtaa aatggtccaa 660

gaagggacac ccaatgatct cttacatcat cctgctacca gtttcgttga acaaatgatt 720

ggggaagagc gtcttcttca tgcgcaggct gatattaccc ctgttaaaca gataatgtta 780

aataatcctg tttcaataac tgctgaaaaa acactaactg aagctattac actaatgcgc 840

caaaaacgcg ttgactcact tctagtaacc gataacggta aattaattgg ttttattgac 900

ttagaatctc taagcagtaa atataagaaa gaccgacttg tttctgatat cttaaaacat 960

actgattttt atgttatgga agacgactta cttagaaata ctgctgagcg tattttaaaa 1020

cgtggtttaa aatacgctcc agttgttgac catgagaata acctaaaggg cattgttact 1080

cgtgcatccc tagttgatat gttatacgat attatttggg gcgatactga aacggaggat 1140

caataa 1146

SeqID 18

atgaaaatag ataaaaaaga atttttagca cttattgcta gtattatttt attaattttt 60

gcttctgtta cgtttttctt atttaaagat catggcacta ctcaaatgga tactgtagaa 120

agcagtgtta accatgtcag tgattctcaa ttaactgagg cacaagatat gttagataaa 180

tttgagaaga aaccttcaga aaaattattg aaggatgtcg aacttgcttt aaataaattg 240

tccaattctt ctaaaaaaga agctttgcaa aaacgtttta agaaagcaaa agataagtat 300

cttaaagacg aagcagataa gaaagctacc aaagatgcta cagatttagt agaaattcta 360

gaacaagccc cttcagaaga aaatgtgtta aaagctgaag cagcagtcaa taagttaact 420

gtcaaagaat ctaaagaagc tttacaaaaa cgtattgata ctgttaagac acaatatggt 480

ttgattggga atcaaactcc ctctagttca gtagctgaaa ctacagaaca agggacagct 540

aatcctgcta gtcaggatac ttctagttac gttaatcaga atgtagcacc aacttatgag 600

caaccgcaaa cgaataatac accagttact ccaggggtta acaatactgt tccgactcca 660

ggaactggta ctgcacctgc tactaatggg acgggtgttg ctcagtaa 708

SeqID 19

atgactaaag atttattatt ggagcttggc cttgaagagt tgccagctta tgttgtgaca 60

ccgagtgaga agcaattggg ccaaaaaatg gtcaagtttt tagaggatca tcgtttatca 120

tttgaaactg ttcaaacttt ttcaactcca cgtcgtttgg ctgttcgtgt taaaggattg 180

gcagaccagc agacagattt aacagaagat ttcaagggtc cttctaagaa aattgctttg 240

gatgcagaag ggaatttttc taaagcagcc caaggttttg ttcgcggaaa aggtttaagt 300

gttgatgata tcgaatttcg tgaagttaag ggagaagaat atgtttatgt cactaagcat 360

gaaactggaa aaagtgcgat tgatgtttta gcaagtgtga cagaggtatt aacagaattg 420

actttcccag tgaacatgca ttgggcaaat aatagttttg aatatattcg tccagttcac 480

actttagtgg tattattaga tgatcaagcc ttagagctag acttccttga tattcattca 540

gggcgtatta gtcgagggca ccgtttcttg ggttctgaca ctgaaatttt atctgctagt 600

tcgtatgaag atgacttgcg acaacaattt gttattgctg atgcaaaaga acgccagcaa 660

atgatcgttg atcaaattca tgctatcgaa gaaaaggaaa atatttcagt tgaaattgac 720

gaagatttac taaatgaagt gcttaattta gttgagtatc cgacagcatt cttgggttct 780

tttgacgaga aataccttga tgtacctgaa gaagttttag tcacttcaat gaaaaatcat 840

caacgctatt ttgttgttcg cgacagggat ggaaaattgt taccaaactt tatttctgtg 900

cgtaatggaa atgctgagca tatcgaaaat gttataaaag gtaatgaaaa agttttagtg 960

gctcgtctcg aagatggtga attcttctgg caagaagatc agaaacttaa tattgcagat 1020

ttagttgaga aattaaaaca agtaactttc catgaaaaaa ttggttcact ttatgaacat 1080

atggatcgtg taaaagttat ttcacaatat ttggctgaga aggcagatct ttctgatgaa 1140

gaaaaattag ctgtgcttcg agctgcaagt atttataaat ttgacctctt aactggaatg 1200

gttgatgagt ttgatgaatt acaaggtatt atgggtgaga aatatgcctt acttgcaggg 1260

gaacagcccg cagtagcagc agcgatacgt gaacattata tgccgacatc ggccgatgga 1320

gagcttccag aaaccagagt tggtgcaatt ctagcgttag ctgataaatt tgacacctta 1380

ctttcattct tctcagttgg tttgattcca agcggttcaa atgatccata tgctcttcgt 1440

cgagctactc aggggattgt ccgtattttg gaagcttttg gttgggatat cccacttgat 1500

gaattagtaa ctaaccttta tggtctatcg tttgctagtt tagattatgc taatcaaaaa 1560

gaggtcatgg cctttatttc agctcgtatt gaaaagatga ttggctctaa ggtccctaaa 1620

gatattcgag aagccgtttt agaatcagac acttatattg ttagccttat tctagaagct 1680

agccaagcat tggtgcaaaa gagcaaggat gctcaatata aagtttccat tgaaagtttg 1740

tcacgtgcat ttaatttagc tgaaaaagtt actcattctg tatcggtgga ctattcattg 1800

tttgaaaata atcaggagaa ggcgctatat caagctattt tgtcacttga actgactgag 1860

gatatgcatg acaacttaga taagcttttc gcacttagtc caattattaa tgatttcttt 1920

gataatacaa tggtaatgac agatgatgaa aaaatgaaac aaaatcgtct ggctttactc 1980

aatagccttg ttgcaaaagc aagaacagtc gctgctttta acttattaaa cacaaaataa 2040

SeqID 20

atgacatttg acaccattga tcaattagcg gttaatacag tccgcacgct ttctattgat 60

gctatccaag cagcaaattc tgggcaccca ggtcttccta tgggagctgc gcctatggct 120

tatgtgcttt ggaataaatt cttaaatgta aacccaaaaa caagtcgcaa ttggacaaac 180

cgtgaccgtt ttgtgctatc agctgggcat ggttcagctc ttctttatag cctacttcat 240

ttagctggct atgatttatc aattgatgat ttaaaacaat tccgccaatg gggatcaaaa 300

acacctggac atccagaagt taaccatact gatggtgtag aagcaacaac tggaccttta 360

gggcaaggta ttgctaatgc tgttggtatg gcaatggctg aagctcattt ggctgctaaa 420

tttaataaac caggctttga tttagttgat cattacactt atacacttca tggtgatggt 480

tgtttgatgg agggtgttag ccaagaagct gccagtcttg ctggacactt aaaacttggt 540

aaattggtcc ttctttatga ttcaaatgat atctcacttg atggtccaac gtctcaatca 600

tttacagaag atgttaaagg gcgttttgaa tcatatggtt ggcaacatat cttagttaaa 660

gatggtaatg atttagaagc gattgctgca gcaattgaag ctgctaaagc tgaaactgat 720

aaaccaacca ttattgaagt taaaactatt attggatttg gcgcagagaa acagggaaca 780

tcttcagttc atggagcgcc acttggtgca gaaggtatta cttttgccaa gaaggcttat 840

ggatgggaat atccagactt tactgttcct gctgaagttg tagctcgatt tgcctcagat 900

cttcaagcgc gtggtgcgaa ggcagaagag gcatggaatg atctttttgc taaatatgaa 960

gttgagtatc cagaattagc agctgaatac aaagaagctt ttgctggcca agctgagaca 1020

gttgaattga aagctcatga cctaggttca tcagtggcta gtcgtgtttc aagccaacaa 1080

gctattcaac aattatcgac tcaattaccg aacctctggg gagggtcagc agacctctca 1140

gcttctaata acacaatggt agcagcagaa acagatttcc aagcaagtaa ttacgctggg 1200

cgtaacattt ggtttggtgt acgtgaattt gcaatggctg ctgcaatgaa tggaattgcg 1260

cttcacggtg ggactcgtgt ttatgggggt acattctttg tcttctcaaa ctacttgtta 1320

ccagcagtac gtatggctgc acttcaaaac ttgccaactg tctatgttat gacacatgat 1380

tctattgctg ttggagaaga tgggccaact catgagccaa tagaacaatt agcatcagtg 1440

cgctcaatgc ctaatttgaa cgttatccgt cccgcagacg gtaatgaaac aaacgctgca 1500

tggcaacgag ctgtatcaga gacagatcga ccaacgatgc ttgttttaac acgtcaaaac 1560

ttaccagttc ttgaaggaac atcagaatta gcccaagaag gggttaataa aggtgcttat 1620

attttatcag aagctaaagg tgaacttgat ggtatcatca ttgcaacagg ttcagaagtt 1680

aaattagctt tagatactca agacaaactt gagtcagagg gcatccatgt tcgcgtcgta 1740

tctatgccag cacaaaacat ttttgatgaa caagaggctt catatcaaga gcaagtgctt 1800

ccatcagctg ttacaaaacg tcttgctatt gaggcaggat caagcttcgg ttggggtaaa 1860

tatgttggtt taaatgggct aacacttact attgatactt ggggagcatc agcaccaggt 1920

aacagaattt tcgaagagta tggctttaca gttgagaatg ctgtatcgct atataaagaa 1980

ctttaa 1986

SeqID 21

atgactttgc aagatcaaat tatcaaagaa ctcggtgtaa aacctgttat taatccaagt 60

caagaaatcc gtcgttccgt agaattttta aaagactatc tattaaaaca tagttttcta 120

aagacctatg tactgggtat ttcagggggg caagattcaa ctttagcagg acgtctagct 180

caactagcag ttgaagagtt gcgtgctgat acaggggaaa actatcaatt tatagctatt 240

cgtttaccat atggtattca ggccgatgaa gaagatgcac aaaaagcatt agattttatc 300

aagccagata ttgctttgac cattaatatt aaagaagctg ttgatggcca agttagagct 360

cttaatgcag caggagtaga gattacagac tttaataaag gaaatattaa agctcgtcaa 420

agaatgatta gtcaatatgc agttgcaggt caatatgctg gagcggttat tggtactgat 480

catgctgcag aaaatattac aggttttttc accaaatttg gtgatggagg tgctgactta 540

ttacctctct ttagacttaa taaaagtcag gggaaacagt tattagctga acttggtgct 600

gataaagcct tgtacgaaaa gattccaacc gcagatttag aagaaaataa accaggaatc 660

gctgatgaaa tagcccttgg agtaacctac caagaaattg atgcttatct tgaagggaag 720

gtggtttcgg acaagtctcg aggtatcatt gaaaattggt ggtataaggg tcaacataaa 780

cgtcatcttc caatcacaat ttttgatgat ttctggaaat aa 822

SeqID 22

attaaaaagg aatctgttat caaactattg aagtatgctt ttggcattat aatgggattt 60

attatcttag ctattgtaat aggtgggctc ctatttgcat actacgttag tcgttctccg 120

aaattaaccg atcaagcttt aaaatccgtt aactctagtt tggtttatga tggtaataat 180

aaacttattg ccgatttagg ctcagaaaag cgtgaaagtg ttagtgcgga tagcattcca 240

ctaaatttgg ttaacgctat cacttctatc gaagataaac gtttctttaa acatagaggt 300

gtcgatattt atcgtatttt aggtgcagct tggcataatc ttgttagtag taatacgcaa 360

ggtggttcaa cccttgatca acagttgatt aaactggctt acttttctac caataaatct 420

gaccaaacgt taaaacgtaa atcacaggaa gtttggcttg cgcttcaaat ggagcgtaaa 480

tacaccaaag aagaaattct tactttctat attaataaag tttatatggg aaatgggaat 540

tatggtatga gaacaacagc taaatcatac tttggtaaag acctaaagga attatctatt 600

gcacaacttg ctttgctcgc tggtattcct caagcaccta cacaatatga cccttataaa 660

aacccagaat ctgctcaaac aagacgtaat accgttcttc agcagatgta tcaagataaa 720

aacatttcta aaaaggaata cgaccaagct gttgcaactc cagtaactga tggcttaaaa 780

gaattaaagc aaaaatctac ttatccaaaa tatatggata actacttaaa acaagttatt 840

agtgaagtta aacaaaaaac tggtaaagat atctttactg ctgggctaaa agtgtatact 900

aatatcaaca ctgatgcaca aaaacaacta tatgacatct acaacagtga cacttacatc 960

gcttatccaa acaatgaatt acaaatagca tctaccatca tggatgcgac taatggtaaa 1020

gtcattgcac aattaggcgg gcgtcatcag aatgaaaata tttcatttgg gacaaatcaa 1080

tctgtcttaa cagaccgcga ttggggttct acaatgaaac ctatctcagc ttatgcacct 1140

gctattgata gtggtgtcta taattcaaca ggtcaatcat taaacgactc agtttactac 1200

tggcctggta cttctactca actatatgac tgggatcgtc aatatatggg ttggatgagt 1260

atgcagaccg ctattcaaca atcacgtaac gtccctgctg tcagagcact tgaagccgct 1320

ggattagacg aagcaaaatc tttccttgaa aaattaggca tatactatcc agaaatgaac 1380

tattcaaatg ctatttcaag taacaacagt agcagtgatg caaaatatgg tgcaagtagt 1440

gagaaaatgg cagcggctta ctcggctttt gcaaacggcg gaacttacta taaaccgcaa 1500

tatgttaata aaattgaatt tagcgatgga accaatgata cttatgcagc gtctggtagc 1560

cgtgcgatga aagagactac tgcctacatg atgacggata tgctgaaaac agtactaaca 1620

tttggtactg gtactaaagc agctatccct ggtgttgcac aagctggtaa gactggtact 1680

tccaactata cggaagatga gttagctaaa attgaagcaa ctactggtat ctacaatagc 1740

gccgttggta caatggctcc tgatgaaaac tttgtcggct atacttctaa gtacacaatg 1800

gcaatttgga ctggttataa aaatcgcctt acaccacttt atggtagcca actggatatt 1860

gctactgagg tttatcgtgc aatgatgtcc tacctaactg gcggatacag tgcagattgg 1920

acaatgccag aagggctcta tcgaagcgga agctacttgt atatcaatgg gacaactaca 1980

accggcactt actcttcttc tgtctataag aatatctatc aaaattctgg acagtcaagt 2040

caaagcagta gttcaacatc ctctgagaaa caaaaagagg ataagaatac agcaaatgat 2100

gccaattcat catccccaca agtcgaaaca cctaataatg gtaatgctac tacccctaat 2160

aattccaatc aaactgtgcc aggaactgga catggtaatg gtaatggtaa caacaataca 2220

gtacctaacg gtaattaa 2238

SeqID 23

atgttagact taaaacgtat tcgtacagat tttgatgttg tcgctaaaaa attggccacg 60

cgtggtgttg accaagaaac attaacaacc cttaaagaac ttgatataaa acgccgtgaa 120

ctacttatca aagctgaaga ggcgaaagcg caacgtaatg ttgcttctgc tgccattgct 180

caagcaaaac gaaataaaga gaatgcagac gagcaaatcg cagctatgca aacactttcc 240

gctgatatca aagcaattga tgcggaattg gcagatgtgg atgctaattt acaatcaatg 300

gtgaccgtac ttcctaatac accagctgat gatgtgccgc ttggagcaga cgaggatgaa 360

aatgttgaag ttcgtcgctg gggaactcct cgtgaatttg attttgagac caaagctcac 420

tgggatttag gtgaaagcct tggaattctc gattgggaac gtggtgcaaa agttactggc 480

tctcgcttcc tcttctacaa aggtctaggt gctcgtttag agcgtgctat ctacagcttc 540

atgctggatg aacacgctaa ggaggggtac actgaagtga tccctcctta tatggttaac 600

cacgattcaa tgttcggtac gggacaatat ccaaagttca aggaagatac tttcgaatta 660

gcagatagcc cattcgtact tatcccaaca gctgaagttc ctttaacaaa ctattatcgt 720

gatgaaatta tcgatgggaa agaattacct atatacttta ctgccatgag tccatcattc 780

cgttcagaag caggctctgc aggacgtgac acacgtggat taattcgcct tcatcaattc 840

cataaagtag aaatggtgaa atttgccaag cctgaggaat cataccaaga attagaaaaa 900

atgacagcaa atgctgaaaa tattcttcaa aaacttaatc tcccataccg tgtcattacc 960

ttgtgtacag gagatatggg attctcagca gctaaaacgt atgacttaga ggtatggatt 1020

cccgctcaaa atacttatcg agaaatttct tcatgttcta atactgaaga tttccaagca 1080

cgtcgtgctc aaattcgtta ccgtgacgaa gttgatggca aagttaggtt gcttcatact 1140

ttaaatggtt caggacttgc agttggacgt actgtagcag caatcctcga aaattatcaa 1200

aatgaagatg gttctgtgac tatcccagaa gttcttcgtc catatatggg taatatcgat 1260

attattaaac caaattag 1278

SeqID 24

gtgacaatat caaatcaaga attgacgctt actcccttaa gaggaaaaag tggcaaagcc 60

tatattggta cttatccaaa tggtgaacgt gtctttgtca aatataatac gacacctatt 120

ttgccagcct tagctaagga acagattgcg ccacaactac tgtgggcacg ccgtacaagt 180

aatggcgata tgatgagtgc tcaagaatgg ttagatggtc gaacattgac taaagaagat 240

atgggtagta aacagattat tcatatttta ttgcgcctgc acaagtctcg tcccttagta 300

aatcagctgt tacaattagg gtataaaatt gaaaaccctt atgatttgtt gatggattgg 360

gaaaaacaaa ctcctattca aatccgggaa aacacttatt tgcaatctat tgtgactgag 420

ttaaagcgta gtttacctga gtttcgaaca gaggttgcaa caatcgttca cggagatatc 480

aaacatagca actgggttat tacaacaagt ggtttaattt atttggttga ttgggattct 540

gttcgtttaa cagatcgcat gtatgacgtt gcatatattt taagtcatta tattccacaa 600

aaacactgga aagattggct gtcttactac ggttataaag ataatgagaa agtctggagc 660

aaaattatct ggtatgggca attttcatat ttatcacaga taatcaaatg ttttgataaa 720

cgagatatgg agcatgtcaa tcaagaaatt tatgaattac gaaagtttag agagttaatt 780

aaaaagcata atgcgagtta g 801

SeqID 25

atgaaaatat cacaatacaa taaatggtca attcgccgat tgaaagttgg agctgcatct 60

gtaatgattg ctagtggaag cattgttgct ttagggcaat ctcatattgt ttcagcagat 120

gagatgtccc aacctaaaac aaccattaca gctccaacag ctaacacttc tacaaatgta 180

gaaagtagta ctgataaggc attatcaaaa gttactacga tggagacaag ttcagaaatg 240

cctaaaatgc aaaatatggc aaaagttgaa aaaacgagtg acaaacctat gatggtggct 300

acctctgtta gaaaaatgat ggcaacgccg acacctgtag cgatgaccaa aacgactagt 360

gtagatgagg taaagaaatc aactgatact gcttttaaac agacagtaga cgttccggct 420

cattatgtta atgcagcaaa agggaatggc ctatttctag caggtgtaaa tcaaacgatt 480

ccatatgaag cgtttggtgg agatggcatg ttaacgcgtt taatccttaa atcttctgaa 540

ggagcaaaat ggtcagataa tggtgttgat aaaaatagtc cattattacc attgaaaggc 600

ttaactaaag gtaaatattt ctatcaagtt tctttaaatg gtaatacgac aggtaaagag 660

ggacaagctc tcttagatca aattaaagct aatgataaac atagctacca agccacaatt 720

agagtttatg gtgctaaaga tggtaaagtt gatttaaaaa acatgattag ccaaaaaatg 780

gtaaccatta acattcctca cattacaact gatatggaag taaaaaattc tctaaagatg 840

gcctttaaag aaaaagtaga cgtcccagca aaatatgtta gtgctgcaaa agctaaaggt 900

ccatttttag caggtgttaa cgaaacgatt ccatatgaag cgtttggtgg ggatggcatg 960

ttaacgcgtt taatccttaa agcttcagag ggagcaaaat ggtcagacaa tggtgttgat 1020

aaaaatagtc cattattacc gttaaaagat ttaactaagg gtaaatactt ctaccaagtc 1080

tctcttaatg gcaatacagc cggtaaaaaa ggccaagctc tcttagatca aattaaggca 1140

aatggaagcc atacttatca agcaactatc actatttatg gtacgaaaga tggcaaagtt 1200

gatatgaata ctattttagg acaaaagaca gtgatgattc atattaatgt cgctaaaaaa 1260

gatatgaata gcacatcaat gatgatgaaa aaagataaga tgacaatgcc tatgaagaaa 1320

gaaatgactt catctaaaat aaataccggt atgatgatgt ctaacaacaa gatgtcagct 1380

aatatgcaaa tgtcatcaca agccaaatca aatgataaag ctggtaaaaa aatgtctatg 1440

atgtctaaaa atttacctaa tacaggtgaa acaaagcaac aaaatgtagg tgtgctaggt 1500

atgcttagtc tagcctttgc gacaggtcta actgcgctag gccttaaaaa atctaaacaa 1560

agatag 1566

SeqID 26

atgacacata ttacatttga ctattcaaaa gtcttgggtc aatttgtagg cgaacacgag 60

ttagactacc tacaaccaca agtaagtgca gcagatgctt tccttcgtca agggactggt 120

cctggctcag attttctcgg atggatggac ctcccagaaa actatgacaa agaagaattt 180

tctcgcattc aaaaagccgc tgaaaagatt aaatcagata gcgaagtact cgtggttatt 240

ggtattggtg gttcgtacct tggcgcaaaa gcagcaattg actttttgaa taatcatttt 300

gctaatttgc aaaccgcaga agaacgtaaa gcgcctcaga ttctttatgc tggaaattct 360

atttcatcta cttaccttgc cgatttagtt gaatacgtcc aagataaaga attctcagta 420

aatgtcattt caaaatcagg tacaacaact gaaccagcga ttgctttccg tgtatttaaa 480

gaacttctag ttaaaaagta cggtcaagaa gaagctaata aacgtatcta cgctacaact 540

gataaagtta aaggtgccgt aaaagttgaa gcagatgcta acaattggga aacatttgta 600

gttcctgata atgttggtgg tcgtttctca gtactgacag ccgtaggtct tcttccaatt 660

gctgcttcag gagcagatat tactgctctt atggaaggcg caaatgctgc tcgtaaagat 720

ttatcttcag ataaaatctc tgaaaacatt gcttatcagt acgctgcagt tcgtaatgtt 780

ctttatcgca aaggttatat cacagagatt ttagctaact acgaaccatc attacaatac 840

tttggtgaat ggtggaaaca attagcaggt gaatcagaag gtaaagatca aaaaggtatc 900

tatcctacat cagcaaactt ctcaactgat cttcactcac ttggtcaatt tattcaagaa 960

ggttaccgta acctctttga gacagttgtt cgcgttgaga aacctcgtaa aaatgtgact 1020

attcctgaat taaccgaaga tttagatggt cttggttatc ttcaaggaaa agatgtagat 1080

tttgttaata aaaaagcaac agatggtgta ctccttgctc atacagatgg tggggttcca 1140

aatatgtttg taacgcttcc tacacaagac gcttacaccc ttggttacac tatttacttc 1200

tttgagttag caattggcct ttcaggttat cttaactcag taaatccatt tgatcaacca 1260

ggggtagaag catataaacg taatatgttt gctcttctag gtaaacctgg attcgaagag 1320

cttagcgctg aattgaatgc acgtctttaa 1350

SeqID 27

atgaaagaaa aacaaacagc cggacgtcgt caattagaag aatttgcacc tgaatttgcc 60

agatataatg atgatatttt atttggtgag gtttgggcaa aagaggatca tttaacagat 120

aaaacacgtt ctattattac tatatctgcc ttaattagcg gaggaaatct tgaacagtta 180

gagcatcatc ttcaatttgc caaacaaaac ggagttacta aagaagaaat tgctgacatt 240

atcactcacc ttgcttttta tgttggatgg ccaaaagctt ggtcagcctt taataaagct 300

aaggaaattt ggatataa 318

SeqID 28

gcttggagag ctttagagga tgctcaaaaa tctggtaagg taaaatccat cggtgtctca 60

aactttttag aaaaagactt agaaaatatc ttaaaaaatg gtcatgttaa acctgctgtc 120

aatcaaatct tagctcacat cggaaataca cctttcgact tgattgatta ctgtcaaagt 180

aaaggtattc aagttgaagc ttattcacca attgcacatg gacaagcatt gaaatctgat 240

ggtattcaaa aaatggctga aaaatatggg gttagtgttg ctcaattatg tattcaatat 300

ttactacaat taaacctgat cgttttacca aaggcttcta gtaaagaaca tttacagtct 360

aatctagact ttgactttgt aattagtgac gaagatatgt cgatattaaa atcacttatg 420

tttgacgatt atggggaatt ttctaacttc cctgttttta gtgaaaaatg a 471

SeqID 29

atgtttagaa ggtctaaaaa taacagttat gatacttcac agacgaaaca acggttttca 60

attaagaagt tcaagtttgg tgcagcttct gtactaattg gtcttagttt tttgggtggg 120

gttacacaag gtaatcttaa tatttttgaa gagtcaatag ttgctgcatc tacaattcca 180

gggagtgcag cgaccttaaa tacaagcatc actaaaaata tacaaaacgg aaacgcttac 240

atagatttat atgatgtaaa gaatggattg attgatcctc aaaacctcat tgtattaaat 300

ccatcaagct attcagcaaa ttattatatc aaacaaggtg ctaaatatta tagtaatccg 360

agtgaaatta caacaactgg ttcagcaact attactttta atatacttga tgaaactgga 420

aatccacata aaaaagctga tggacaaatt gatatagtta gtgtgaattt aactatatat 480

gattctacag ctttaagaaa taggatagat gaagtaataa ataatgcaaa tgatcctaag 540

tggagtgatg ggagtcgtga tgaagtctta actggattag aaaaaataaa aaaagatatt 600

gataataatc caaaaacaca aatagatatt gataataaaa ttaatgaagt caatgaaata 660

gagaaattgt tagttgtatc gctaccagat aaaattaagt attcaccaga ggctaagcat 720

aggactgttg aacaacacgc ggaattagat gcaaaagata gcattgcaaa tacagatgaa 780

ttgccatcaa attcaacgta taactggaaa aatggtcata aaccagacac ctcaacatca 840

ggtgaaaaag acggaattgt tgaagttcac tatccagatg gtactgttga tgatgtgaat 900

gttaaagtaa ccgttacatc gaaaaaaact gataatacag ctccaacatt aaccgtcact 960

ccagagcaac agactgttaa agtggatgaa gatattacct ttacggttac agctgaagac 1020

gaaaatgaag ttgaactagg tttagatgat cttaaagcta agtatgaaaa tgatatcatt 1080

ggagctcgtg ttaaaattaa gtatcttact aaagaaccta ataagaaagt catggaagtg 1140

acaattatga aagctacttt agcagataag ggcgcaatta cctttactgc aaaagataaa 1200

gcaggtaatc aagcagaacc taagacagtt accatcaatg ttcttccgcc agataaaatt 1260

aagtattcac cagaggctaa gcataggact gttgaacaac acgcggaatt agatgcaaaa 1320

gatagcattg caaatacaga tgaattgcca tcaaattcaa cgtataactg gaaaaatggt 1380

cataaaccag acacctcaac atcaggtgaa aaagacggaa ttgttgaagt tcactatcca 1440

gatggtactg ttgatgatgt gaatgttaaa gtaaccgtta catcgaaaaa aactgataat 1500

acagctccaa cattaaccgt cactccagag caacagactg ttaaagtgga tgaagatatt 1560

acctttacgg ttacagctga agacgaaaat gaagttgaac taggtttaga tgatcttaaa 1620

gctaagtatg aaaatgatat cattggagct cgtgttaaaa ttaagtatct tactaaagaa 1680

cctaataaga aagtcatgga agtgacaatt atgaaagcta ctttagcaga taagggcgca 1740

attaccttta ctgcaaaaga taaagcaggt aatcaagcag aacctaagac agttaccatc 1800

aatgttcttc cgccagataa aattaagtat tcaccagagg ctaagcatag gactgttgaa 1860

caacacgcgg aattagatgc aaaagatagc attgcaaata cagatgaatt gccatcaaat 1920

tcaacgtata actggaaaaa tggtcataaa ccagatacct caacaccagg agagaaaaac 1980

gctgttgttg ttgtgaccta tccagataag tcaacagatg aagttcctgt aaaagttaca 2040

gtggttgacc caagtacaga cgcagagaaa aatgatccag caggtaaaga tcaaactgtc 2100

aaagtgggtg aacaaccaga tccgacaaaa tctcttgaag cagtaccagc tggaagtaca 2160

gtagcgtaca aagaaccagt tgatactaag acaccaggag agaaaaacgc tattgttgtt 2220

gtgacctatc cagataagtc aacagatgaa gttcctgtaa aagttacagt ggttgaccca 2280

cgtacagacg cagagaaaaa tgatccagca ggtaaagatc aaactgtcaa agtgggtgaa 2340

caaccagatc cgacaaaatc tcttgaagca gtaccagctg gaagtacagt agcgtacaaa 2400

gaaccagttg atactaagac accaggagag aaaaacgcta ttgttgttgt gacctatcca 2460

gataagtcaa cagatgaagt tcctgtaaaa gttacagtgg ttgacccacg tacagacgca 2520

gagaaaaatg atacagcagg taaagatcaa actgtcaaag tgggtgaaca accagatccg 2580

acaaaatctc ttgaagcagt accagctgga agtacagtag cgtacaaaga accagttgat 2640

actaagacac caggagagaa aaacgctgtt gttgttgtga cctatccaga taagtcaaca 2700

gatgaagttc ctgtaaaagt tacagtggtt gacccacgta cagacgcaga gaaaaatgat 2760

ccagcaggta aagatcaaac tgtcaaagtg ggtgaacaac cagatccgac aaaatctctt 2820

gaagcagtac cagctggaag tacagtagcg tacaaagaac cagttgatac taagacacca 2880

ggagagaaaa acgctgttgt tgttgtgacc tatccagata agtcaacaga tgaagttcct 2940

gtaaaagtta cagtggttga cccacgtaca gacgcagaga aaaatgatcc agcaggtggg 3000

gaaacaacag taccacaagg cacaccgatc agtgatgaag aaattacagg attagtgaag 3060

attccagaag gatcaaacgg cgtaccgaaa gtagtaggag accgtccgaa tacagacgta 3120

ccaggagatt ataaagtaac ggtagaagta acgtatccag atggaacaaa ggatacagta 3180

gcagtaacgg ttcatgtgac accaaaacca gtaccggata aagataaata tgatccaaca 3240

ggtaaatctc agcaagtcaa cggtaaagga aataaactac cagcaacagg tgagagtgca 3300

actccattct ttaatgttgc agctttgaca attatatcat cagttggttt attatctgtt 3360

tctaagaaaaaagaggatta a 3381

SeqID 30

atgcagattc tcgaagacta tgatggtaga gcattaccca agctagaaac ggatcgactt 60

atcttgcgtc agcgaacggt gggggatgtc ccagccatgt ttgattacgt ttgcttggaa 120

gaggttgcct atccagcagg gctcagccca atagcatctt tagaagatga gtatgattat 180

tttgaaaatc gctactatca aaatctggaa aaggcaaaat taccatctgg ttatggtatt 240

acggttaaag gaagtgaccg gattatcgga tcctgcgcct ttaaccatcg ccgtgaagac 300

gatgttttcg agattggcta tctgcttcat cctgactact ggggtcatgg ctatatgaca 360

gaagctgtcg ccgctttgat tgaagtcggt tttacccttc tcaatctcca caaaatcgaa 420

atccgctgct atgactacaa taagcaaagc cagcgcgtgg cagaaaaact aggattcacc 480

cttgaagcaa ccatccgtga tcgtaaggat aatcaaggca atcgctgtgt caatttgata 540

tatggtttgc tgaggagtga gtgggagtaa 570

SeqID 31

atgtcaaaag aactatcacc aaaatacaat cctgccgagg ttgaggaagg ccgttatcaa 60

acttggcttg accaagatgt tttcaagcca tcaggggata ctgaggctaa gccctattct 120

atcgtgattc caccaccaaa cgttacgggt aaattacact tgggtcacgc ttgggataca 180

actctacaag atatcattat tcgtcaaaag cgtatgcagg gctttgatac actctggctt 240

ccaggtatgg accacgctgg tatagccact caagctaagg ttgaggagcg ccttcgtgag 300

caaggtattt cacgttatga tcttggtcgt gaaaaattcc tagataaagt ttgggaatgg 360

aaagacgagt atgcagcaac tattaaatct cagtggggga aaatgggtct ttcggttgat 420

tatagccgtg agcgttttac ccttgatgag ggactttcaa aagcggtaag aaaagtcttt 480

gttgatttgt acaataaagg ttggatctat cgtggggaat tcatcattaa ctgggatcca 540

gcagctcgta cagccctttc agatattgaa gttattcaca aggatgttga gggtgctttc 600

tatcatatga attatatgct ggaagatggc tcacgcgccc tagaagtagc aacaactcgt 660

cctgagacta tgtttggaga tgtggctgta gcggtaaatc cagaagatcc acgttacaaa 720

gatttgattg gtcaaaatgt tatccttcct atcatcaata aacctattcc aatcatcgct 780

gatgaacacg ctgatccaga atttggaaca ggtgtggtta agattacgcc ggctcacgac 840

cctaatgact tcgctgtagg tcaacgccac aacttaccac aagttaacgt tatgaacgat 900

gatggtacta tgaatgaact tgcggatgaa tttaatggta tggatcgttt cgaagctcgt 960

aaggcagtag tcgctaaatt agaatcgtta ggaaacttag taaaaattga aaaaatgact 1020

cattctgttg gtcattcaga acgaacaggc gttgtggttg aaccacgttt atctactcaa 1080

tggttcgtca agatggatca attggctaag aatgccattg ccaaccaaga tacagaagac 1140

aaggtagagt tttatccacc acgatttaac gatactttta tgagctggat ggaaaatgtc 1200

catgactggg ttatctctcg tcaattatgg tggggacacc aaattcctgc atggtacaac 1260

gtcaatggtg agatgtatgt tggagaagat gccccagagg gtgacggatg gacacaggat 1320

gaagacgttc tagacacatg gtttagttct gctttatggc cgttttcaac aatgggatgg 1380

ccagacaccg aagcagctga cttcaaacgc tacttcccaa catcaacctt ggtaactgga 1440

tacgatatca tcttcttctg ggtatcgcgc atgattttcc aatctcttga atttacagga 1500

cgccaaccat tctcaaatgt tcttattcat ggactcatcc gtgatgaaga aggacgtaaa 1560

atgtctaaat cacttggcaa cgggattgat ccgatggatg tcattgagaa atatggtgcg 1620

gatgcccttc gttggttcct ctcaaatggt tcagcccctg gtcaagatgt gcgtttttca 1680

tacgaaaaaa tggatgcttc gtggaatttc attaataaga tttggaacat ttcacgctat 1740

atccttatga ataacgaagg tttaacgttg gatcaagcca gagaaaatgt ggaaaaagtt 1800

gttaatagtc aagttggaaa tgtaacagac cgttggattc tccacaattt aaatgagact 1860

gtgggtaaag ttactgaaag ctttgacaag tttgaatttg gtgttgctgg tcacatcctt 1920

tacaacttta tctgggaaga atttgctaat tggtacgttg agttaacaaa agaggttctt 1980

tatagtgaca atgaagatga gaaagttgtt acacgttctg ttcttcttta taccttagat 2040

caaatcttgc gtcttcttca tccaatcatg ccatttgtga cagaagaaat ttttggacaa 2100

tacgcagaag gatcaattgt ccttgcaagt tatccacaag ttaacgcgac atttgaaaat 2160

cagacagctc acaaaggcgt tgaaagcttg aaagatttga ttcgttcagt gcgaaatagc 2220

agagcagagg tcaatgttgc tccatctaaa ccaatcacta tcttggttaa gacaagtgac 2280

agcgaactcg aaagcttctt caaagacaat agtaactaca tcaaacgctt cacaaaccca 2340

gagacgcttg aaatcagctc agcgattact gccccagaac ttgccatgac tagcattatc 2400

acaggtgcag aaattttcct cccacttgct gacctcctta acgttgaaga agagttggca 2460

cgacttgaaa aagaacttgc taaatggcaa aaagaactaa atatggtcgg caaaaaactc 2520

agcaacgagc gctttgtagc taacgctaaa ccagaagtcg tccaaaaaga aaaagacaaa 2580

cagaccgact accaaactaa atacgacgca actatagcac gtattgaaga gatgaaaaaa 2640

ttaaataatg attga 2655

SeqID 32

atggttgagc caattatttc aatacaagga cttcataaaa gttttgggaa aaatgaggtt 60

ttaaaaggca ttgacttgga tattcatcaa ggagaagtgg tggttattattggcccttct 120

ggctctggta agtcaacatt tttaagaaca atgaatctct tggaagtacc aacaaaggga 180

acagtgactt ttgaagggat tgatataaca gacaaaaaga atgatatttt taaaatgcgc 240

gaaaaaatgg gcatggtttt tcaacagttc aatctatttc ccaatatgac tgtactagaa 300

aatattactt tatcacctat taagacaaag ggactttcta agcttgatgc tcagacaaaa 360

gcatacgagc tacttgaaaa agttggactc aaagagaagg ctaatgctta tccagctagc 420

ttatctggag ggcaacaaca acgaattgct attgcaagag gtcttgcaat gaatcctgat 480

gtccttcttt ttgatgaacc tacttcagct cttgatcctg aaatggtagg tgaagtcttg 540

actgttatgc aagatttagc taaatctggt atgacgatgg ttattgtcac tcatgaaatg 600

ggttttgcac gtgaagtagc ggatcgtgtc atttttatgg atgcaggcat tattgttgag 660

caagggaccc ctaaggaagt atttgagcag acaaaagaaa tccgcacaag agatttctta 720

agtaaagtat tataa 735

SeqID 33

atgaattata aagagattta tcaagagtgg ttagaaaacg actcactcgg taaagatatt 60

aagtcagatt tagaagctat taaaggcgat gaatctgaaa ttcaggatcg tttttacaaa 120

acattagaat ttggaacggc gggattgaga ggtaaacttg gagcaggaac caatcgtatg 180

aatacttata tggtggggaa agcagcacaa gcattagcta atacgattat tgatcatggc 240

cctgaagcta ttgcacgtgg aattgcagtt agttatgatg tccgttatca atctaaggaa 300

tttgcagaat taacttgttc cattatggca gcaaatggta ttaagtctta tatttataaa 360

gggattcgcc caacaccaat gtgctcatat gctattcgtg ctctaggatg tgtttcgggt 420

gtgatggtta ctgctagtca taatcctcaa gcttataatg gttataaggc atattggaaa 480

gaaggatctc agattttaga tgatattgct gatcaaattg ccaatcatat ggatgctata 540

accgattatc agcaaattag gcaaataccc tttgaagagg ctctggcaag tggtttggca 600

agttatattg atgagagtat tgaagaagca tataaaaaag aagtgcttgg tttaaccatt 660

aatgatacta acattgataa gtcagtccga gtagtttata ctcctttaaa tggcgtagga 720

aatttacctg tgcgcgaagt tttaagacgc cgtggttttg aaaatgttta tgtggtacct 780

gagcaggaaa tgcccgatcc tgattttaca acggttggct atccaaaccc tgaagttcct 840

aaagcatttg cctattcaga atctctagga aagtcagttg atgcagatat cttacttgcc 900

acagatccag attgtgaccg agtagcattg gaagtcaaag atagtaaggg agaatatatt 960

ttcttaaatg gtaataagat aggggcactt ctttcctatt atattttttc acaacgatgt 1020

gccttaggga atttgccaca tcatcctgta ttggtaaaat ccattgtaac tggtgatcta 1080

tcaaaagtta ttgcagataa atataatatt gaaactgttg aaactttaac cggatttaaa 1140

aatatttgtg gaaaagctaa tgaatatgat atctcaaagg ataaaactta tctctttggc 1200

tatgaagaaa gtattggttt ttgctatggc acttttgtac gtgataaaga tgctgtgagt 1260

gcttcaatga tggtagtaga aatgactgcc tattataaag aacgagggca aacactttta 1320

gacgttttgc aaaccattta cgatgaattt ggctattaca acgagcgcca attttctctt 1380

gagttagagg gtgctgaggg gcaagaacgt attagtcgta ttatggagga ttttagacag 1440

gacccaatat tacaagtagg tgagatgaga ttggagaatt ctattgattt caaggatggt 1500

tataaggatt ttccaaagca aaattgttta aaatattatt ttaatgaggg ttcatggtat 1560

gctttaaggc cgtcagggac ggaacctaag ataaaatgtt acctttatac gattggttgt 1620

acagaagcag atagtttatc gaaacttaat gcaattgagt cggcttgtcg tgctaaaatg 1680

aatagtacta aataa 1695

SeqID 34

atgtatagag aaattaccgc tgtcgaacac gatcgctttg tgagcgaatc caaccaaaca 60

aacctacttc aatcttctaa ttggcccaaa gtaaaagaca actggggtag tcaattactt 120

ggcttttttg acggtgaaac ccaaattgcc agcgctagta ttctcatcaa atcacttcct 180

cttggcttct ccatgctata tattccgcgt ggaccaatca tggattactc caatctagat 240

attgtaacta aggtccttaa ggaccttaaa gcttttggca aaaaacaaag agctctcttt 300

atcaagtgtg atcctctcat ctatttaaaa atggtcaatg ccaaagattt tgaaaattcg 360

cccgatgaaa aagaagggtt aatcgccatt gatcatttac agcgtgcagg tgctgattgg 420

actggtcgga caacagattt agctcatact atccaaccac gttttcaggc aaacctatat 480

gctaatcaat ttggacttga taaaatgtct aaaaaaactc gtcaagctat tcgaacttcc 540

aaaaataagg gagtagatat acaatttggt agccatgaac tacttgaaga ttttgcagag 600

ttgatgaaaa aaacggaaga ccgtaaaggg ataaatctta gaggaattga ctactaccaa 660

aaactccttg atacgtatcc taataactca tacatcacaa tggcttcgct agatgttgca 720

aaacgattag aaaaaataga aaaagaatgt cagatagccc aatctgaaag aataaaatca 780

cttgaactta atcgtgagaa aaaagttaaa caacaccaag gtacgattga tcgattaaat 840

aaagaaattg attttctcaa agaagctcaa aaagcctatg accgagacat tattccattg 900

gctgcaaccc tcactctaga atttggtaat acatcagaaa atatttatgc tggtatggac 960

gattacttta aatcttattc tgctcctatt tacacttggt ttgaaacagc tcaaagggct 1020

tttgaacgtg gaaatatttg gcaaaatatg ggggggattg agaatgactt atctggtggt 1080

ttatatcatt ttaagtctaa gttcgaacca atcattgagg aatttattgg agaatttaac 1140

attccagtga acagattgct ttataaagcc tctaattatg tctatgcctt acgcaaaaaa 1200

cgtaatagct ag 1212

SeqID 35

atggcttgta caacaatatt ggttggtaaa aaggcttctt atgatggttc gactatgatc 60

gctagaacgg aagactctgt taatggcgat ttcacaccca aaaaattaaa ggtaatgaca 120

tctaaagatc aaccgcgtca ttacaaatca gttttatcaa attttgaagt agatttacca 180

gataacccac ttccttatac ttcagtaccg gacgcattgg gaaaagatgg tatatggggt 240

gaagccggta ttaacagtaa aaatgtagcg atgagtgcta cagaaactat tacaacgaat 300

tcccgcgttt tgggtgcaga tcctttggtt tcagatggta taggggaaga ggatatactc 360

actttagtgc ttccctatat tcagtcagcg cgagaaggtg tggagcgttt aggtgctatt 420

ttggaaaaat atggaaccta tgaatcaaat ggtattgctt tttcagatac cgaagaaata 480

tggtggttag aaacaattgg tgggcatcat tggattgctc gtcgcgtacc tgatgatgtt 540

tatgttacta atcctaacca actaggaatt gatcattttg aatttaataa ctgtgatgac 600

tacatgtgct ctagtgattt gaaagagttt atcgaacaat accatttaga tttgacctat 660

tctaatgagc atttcaatcc tcgatatgct tttggtagcc aacgtgataa agatcgtcat 720

tacaacacac caagaagttg ggcaatgcag cgttttttaa atcctgaaat tgaacaggat 780

ccacgtagct tgtttattcc ctggtgtcaa aagccttacc gaaaaattac tgttgaggat 840

attaaatatg tgttgagtga tcattatcaa gacagtgtgt atgacccata tggaccagaa 900

ggggatgcgg taagtaggag agcttttcgt tcagttggta tcaaccgaac tagtcaaacg 960

tctattctac aattacgacc aaataaatca cttgaaacga caggtgttca atggttatct 1020

tatggctcta tgccatttgc aaccatggtg ccgttgttta cacaagttga gactgtacca 1080

aactattttt cgaatacaac caaggatgct tcaacagata atttttattg gaccaatcgt 1140

ttaattgcag ctctagcaga tccacacttt tatcaacatg aagctgatat tgaaagctat 1200

atcgagagaa cgatggctca aggacatgca catattaacg gtgttgatag agaagttgct 1260

gagaataaag agattgattt tcaacagaaa aatcaagaaa tgagtgacta tatccaaaaa 1320

gaaagccaag aattgttaaa tcgtattcta tttgatgcaa gtaatttaat gacaaatcgc 1380

ttttcaatgg gagattaa 1398

SeqID 36

atgagaaaga aatttctttt attgatgagc tttgtagcta tgtttgcagc ttggcaactt 60

gttcaagtta aacaagtttg ggctgatagt aaacttaaag tggtaacaac tttttaccca 120

gtttatgagt ttacaaaaaa tgtcgttggt gataaagctg atgtatctat gttaattaaa 180

gcaggtacag aaccgcatga ttttgaacca tcaactaaaa acatcgctgc catccaagat 240

tcaaatgctt ttgtttacat ggatgataac atggaaactt gggctccaaa agtagctaag 300

tcagttaaat ccaaaaaagt aacaactatt aaaggtactg gcgatatgtt acttactaaa 360

ggcgtcgaag aagaaggtga agaacatgaa ggacatggtc atgaagggca tcatcatgaa 420

cttgacccac acgtatggtt gtctccagaa cgtgcgattt ctgttgtaga aaacatccgt 480

aataaatttg tcaaagctta tccaaaagat gcagcttcat ttaacaaaaa tgcagatgct 540

tacattgcaa aattaaaaga gcttgacaaa gaatacaaaa atggtttgtc aaatgctaaa 600

caaaagagtt ttgtgactca acacgcagcg tttggttaca tggcgcttga ttacggttta 660

aatcaagttc caattgctgg tcttactcca gatgcagaac cttcatcaaa acgtttaggc 720

gaattagcta aatacatcaa gaaatataac atcaactaca tttattttga agaaaatgct 780

tcaaataaag ttgctaaaac tttagcagat gaagttggcg tgaaaacagc tgtgcttagt 840

ccacttgaag gactttctaa aaaagaaatg gcagctggcg aagattactt ctcagttatg 900

agacgtaatt tgaaagttct taaaaagaca acagatgttg caggtaaaga agtagctcct 960

gaagaagata aaactaaaac agttgaaaca ggttacttta aaactaaaga tgttaaagac 1020

cgtaaattga cagattactc tggtaattgg caatcagtat atcctcttct tcaagatggg 1080

acacttgatc cagtttggga ttacaaagct aaatctaaaa aagatatgac tgctgcagag 1140

tacaaaaaat attatacagc aggttacaag actgacgtag aatcaatcaa gattgatggt 1200

aaaaaacatc aaatgacctt tgtacgtaat ggtaaatcac aaacatttac atacaaatat 1260

gcaggttaca aaatcttaac ttataaaaaa ggtaatcgtg gagtacgtta tctctttgaa 1320

gctaaagaaa aagatgctgg tcaattcaaa tatatccaat ttagtgacca tggtattaaa 1380

ccgaataaag ctgaacactt ccatatcttc tggggttcag aaagccaaga aaaattattt 1440

gaggaaatgg aaaactggcc aacatacttc ccagctaaaa tgtctggacg tgaagttgcc 1500

caagacctta tgtctcatta a 1521

SeqID 37

atgaaattat cgaagaagtt attgttttcg gctgctgttt taacaatggt ggcggggtca 60

actgttgaac cagtagctca gtttgcgact ggaatgagta ttgtaagagc tgcagaagtg 120

tcacaagaac gcccagcgaa aacaacagta aatatctata aattacaagc tgatagttat 180

aaatcggaaa ttacttctaa tggtggtatc gagaataaag acggcgaagt aatatctaac 240

tatgctaaac ttggtgacaa tgtaaaaggt ttgcaaggtg tacagtttaa acgttataaa 300

gtcaagacgg atatttctgt tgatgaattg aaaaaattga caacagttga agcagcagat 360

gcaaaagttg gaacgattct tgaagaaggt gtcagtctac ctcaaaaaac taatgctcaa 420

ggtttggtcg tcgatgctct ggattcaaaa agtaatgtga gatacttgta tgtagaagat 480

ttaaagaatt caccttcaaa cattaccaaa gcttatgctg taccgtttgt gttggaatta 540

ccagttgcta actctacagg tacaggtttc ctttctgaaa ttaatattta ccctaaaaac 600

gttgtaactg atgaaccaaa aacagataaa gatgttaaaa aattaggtca ggacgatgca 660

ggttatacga ttggtgaaga attcaaatgg ttcttgaaat ctacaatccc tgccaattta 720

ggtgactatg aaaaatttga aattactgat aaatttgcag atggcttgac ttataaatct 780

gttggaaaaa tcaagattgg ttcgaaaaca ctgaatagag atgagcacta cactattgat 840

gaaccaacag ttgataacca aaatacatta aaaattacgt ttaaaccaga gaaatttaaa 900

gaaattgctg agctacttaa aggaatgacc cttgttaaaa atcaagatgc tcttgataaa 960

gctactgcaa atacagatga tgcggcattt ttggaaattc cagttgcatc aactattaat 1020

gaaaaagcag ttttaggaaa agcaattgaa aatacttttg aacttcaata tgaccatact 1080

cctgataaag ctgacaatcc aaaaccatct aatcctccaa gaaaaccaga agttcatact 1140

ggtgggaaac gatttgtaaa gaaagactca acagaaacac aaacactagg tggtgctgag 1200

tttgatttgt tggcttctga tgggacagca gtaaaatgga cagatgctct tattaaagcg 1260

aatactaata aaaactatat tgctggagaa gctgttactg ggcaaccaat caaattgaaa 1320

tcacatacag acggtacgtt tgagattaaa ggtttggctt atgcagttga tgcgaatgca 1380

gagggtacag cagtaactta caaattaaaa gaaacaaaag caccagaagg ttatgtaatc 1440

cctgataaag aaatcgagtt tacagtatca caaacatctt ataatacaaa accaactgac 1500

atcacggttg atagtgctga tgcaacacct gatacaatta aaaacaacaa acgtccttca 1560

atccctaata ctggtggtat tggtacggct atctttgtcg ctatcggtgc tgcggtgatg 1620

gcttttgctg ttaaggggat gaagcgtcgt acaaaagata actaa 1665

SeqID 38

atgaaaaaga gacaaaaaat atggagaggg ttatcagtta ctttactaat cctgtcccaa 60

attccatttg gtatattggt acaaggtgaa acccaagata ccaatcaagc acttggaaaa 120

gtaattgtta aaaaaacggg agacaatgct acaccattag gcaaagcgac ttttgtgtta 180

aaaaatgaca atgataagtc agaaacaagt cacgaaacgg tagagggttc tggagaagca 240

acctttgaaa acataaaacc tggagactac acattaagag aagaaacagc accaattggt 300

tataaaaaaa ctgataaaac ctggaaagtt aaagttgcag ataacggagc aacaataatc 360

gagggtatgg atgcagataa agcagagaaa cgaaaagaag ttttgaatgc ccaatatcca 420

aaatcagcta tttatgagga tacaaaagaa aattacccat tagttaatgt agagggttcc 480

aaagttggtg aacaatacaa agcattgaat ccaataaatg gaaaagatgg tcgaagagag 540

attgctgaag gttggttatc aaaaaaaatt acaggggtca atgatctcga taagaataaa 600

tataaaattg aattaactgt tgagggtaaa accactgttg aaacgaaaga acttaatcaa 660

ccactagatg tcgttgtgct attagataat tcaaatagta tgaataatga aagagccaat 720

aattctcaaa gagcattaaa agctggggaa gcagttgaaa agctgattga taaaattaca 780

tcaaataaag acaatagagt agctcttgtg acatatgcct caaccatttt tgatggtact 840

gaagcgaccg tatcaaaggg agttgccgat caaaatggta aagcgctgaa tgatagtgta 900

tcatgggatt atcataaaac tacttttaca gcaactacac ataattacag ttatttaaat 960

ttaacaaatg atgctaacga agttaatatt ctaaagtcaa gaattccaaa ggaagcggag 1020

catataaatg gggatcgcac gctctatcaa tttggtgcga catttactca aaaagctcta 1080

atgaaagcaa atgaaatttt agagacacaa agttctaatg ctagaaaaaa acttattttt 1140

cacgtaactg atggtgtccc tacgatgtct tatgccataa attttaatcc ttatatatca 1200

acatcttacc aaaaccagtt taattctttt ttaaataaaa taccagatag aagtggtatt 1260

ctccaagagg attttataat caatggtgat gattatcaaa tagtaaaagg agatggagag 1320

agttttaaac tgttttcgga tagaaaagtt cctgttactg gaggaacgac acaagcagct 1380

tatcgagtac cgcaaaatca actctctgta atgagtaatg agggatatgc aattaatagt 1440

ggatatattt atctctattg gagagattac aactgggtct atccatttga tcctaagaca 1500

aagaaagttt ctgcaacgaa acaaatcaaa actcatggtg agccaacaac attatacttt 1560

aatggaaata taagacctaa aggttatgac atttttactg ttgggattgg tgtaaacgga 1620

gatcctggtg caactcctct tgaagctgag aaatttatgc aatcaatatc aagtaaaaca 1680

gaaaattata ctaatgttga tgatacaaat aaaatttatg atgagctaaa taaatacttt 1740

aaaacaattg ttgaggaaaa acattctatt gttgatggaa atgtgactga tcctatggga 1800

gagatgattg aattccaatt aaaaaatggt caaagtttta cacatgatga ttacgttttg 1860

gttggaaatg atggcagtca attaaaaaat ggtgtggctc ttggtggacc aaacagtgat 1920

gggggaattt taaaagatgt tacagtgact tatgataaga catctcaaac catcaaaatc 1980

aatcatttga acttaggaag tggacaaaaa gtagttctta cctatgatgt acgtttaaaa 2040

gataactata taagtaacaa attttacaat acaaataatc gtacaacgct aagtccgaag 2100

agtgaaaaag aaccaaatac tattcgtgat ttcccaattc ccaaaattcg tgatgttcgt 2160

gagtttccgg tactaaccat cagtaatcag aagaaaatgg gtgaggttga atttattaaa 2220

gttaataaag acaaacattc agaatcgctt ttgggagcta agtttcaact tcagatagaa 2280

aaagattttt ctgggtataa gcaatttgtt ccagagggaa gtgatgttac aacaaagaat 2340

gatggtaaaa tttattttaa agcacttcaa gatggtaact ataaattata tgaaatttca 2400

agtccagatg gctatataga ggttaaaacg aaacctgttg tgacatttac aattcaaaat 2460

ggagaagtta cgaacctgaa agcagatcca aatgctaata aaaatcaaat cgggtatctt 2520

gaaggaaatg gtaaacatct tattaccaac actcccaaac gcccaccagg tgtttttcct 2580

aaaacagggg gaattggtac aattgtctat atattagttg gttctacttt tatgatactt 2640

accatttgtt ctttccgtcg taaacaattg taa 2673

SeqID 39

ggagcaaaaa aggcagggtg gactgaatac gctaggatgt tagaagtcag agagcaggtt 60

gaccatgtga tgattccaaa aatcaatcag gatttaccaa tctacgctgg tccagaagag 120

gacaatctgc aacggggagt tggtcatcta gaagggataa gtttgccgat tggaggggct 180

tctacacatg cggtcttgag cggtcaaaga ggtatgccag ctgctcggtt gtttgcggat 240

ttggataaga tgaaaaaagg tgattatttt tatgttacca atctgaaaga aaccttggct 300

tatcaagtgg atcgtatcat ggtgattgaa cctagccaat tggatgccgt gagcattgaa 360

gaggataaag attatgttac ccttctgacc tgtacacctt atatgggctc tttgtcaact 420

gtaatgggtg acttatcatt aacaacgaga gagaatcagc ttggttctct ctctttttgg 480

atgttcaaag cgatgagaat tttgctttta aaatttttaa agttgcgaaa gccaaaggct 540

tgtcgcttga tgtctttgat aagcttgttg gtcgcttcta atttggcatt ggaaagggat 600

agctga 606

SeqID 40

atgtcggatg ttgtcgaaaa acaaacagct aaatcgttta taatgaacgt attaaatggt 60

ttagctttag ggactgttat tgtcttgata ccaggagcaa tccttggaga attaatgaaa 120

gctttactgc caatgtggag cggatttgca actttaatcg ctgcaacagc agttgcaaca 180

agtatgatgg ggcttgttat tggtattatg gttggtctca attttaaatt taatcccatt 240

caatcagcat cactaggatt agcggttatg tttgcaggtg gagctgcaac cttccttaaa 300

ggcgctatta tgctaaaagg aacaggcgat atcattaata tgggaattac tgctgcctta 360

ggtgtccttt tgatccaatt tttatctgac aagacgaaat cattcacttt gattgtcatt 420

ccaacagtaa cactcttact agttggagga gtaggtcatg tgttattacc ttatgtcaaa 480

atgattacca ccatgattgg acaagggatt gcttcattac ttggtttaca acctgttttg 540

atgtcaattt taattgcgat gattttctgt ttcttgatcg tatctccaat cacaacagtt 600

gggattgctt tggcaattag tttgtccgga attggttctg gtgctgctaa tttaggtatc 660

tgtgctgcta gttttgggct ttgtatggct gggtggtcag tcaattctaa ggggacagcc 720

ttagcgcatg ttttagggtc tcctaagatt tcgatggcta atgtattagc aaaaccgaag 780

attatgctac ctatgatatc ttcagcagca atactaggaa ttctcggagc tctctttaat 840

attcaaggaa caccagctag tgcaggtttt ggtatcagtg gtttgattgg accgattaat 900

gctcttaatt tagcaaaagg tggatggtca gttatgaata tgttattgat aattataata 960

tttgttgctg caccaattat attgaatttt atttttaatt acctctttat aaaagtactt 1020

aaaattattg atccaatgga ctataaatta gatatttaa 1059

SeqID 41

atggctagac ccttagtaga acaaacagca gatcgtctat tgcatttaat tttagaaaga 60

gaataccctg ttggtgctaa gctccccaat gaatatgaat tagcggaaga tctagatgtt 120

ggtcgcagca caatccgaga agcagtcaga agtttagcaa cgcgtaatat tttagaagtc 180

agacagggct ctggaacata tattagttct aaaaagggtg tttctgagga tccattaggg 240

ttctctctaa taaaggacac agataggttg acatcagatc tctttgaact acgcctttta 300

ttggaacctc gaatagcgga gctggtagct tatagaatta cagatgatca gttacaatta 360

ctcgaaaagc ttgttggaga tattgaagat gctgttcacg caggcgatcc gaaacattta 420

cttttagatg tggaatttca ttccatgcta gctaaataca gtggtaatat tgcaatggat 480

agccttttgc cagtcatcaa ccagtcaatt catctgataa atgcgaatta tacaaatcgt 540

cagatgaagt cggatagttt agaagcacat agagaaatta tcaaagcaat ccgagaaaaa 600

aatccagtag ctgcacatga tgctatgctt atgcatatca tgagtgttag acgttcagca 660

ttgaaagctt aa 672

SeqID 42

atgattaaga aaaataaagt ttttttagga gttcttttag tccttgttgt tatccttgga 60

ggtggtgtat tattttacca atcacaattc caaaagacaa ctaatcaagc gttagctatt 120

gcttataaag atgctaaagt ggctaagaag gatgtcatac atcaaaaaat tgacaaagaa 180

tttgaaaact ttagagggag ctacgaaatt gagttcaata ccaaaagcgc agaatacagc 240

tatcatgtag atgctaagac tggacaaatc cttgagcggg acatggataa taatggcttt 300

tcaaaatcga cttctcagtc aagttcatcg tcaagtcaaa aatctcataa aataagtcaa 360

gaagaagcca aaaagattgc atttaaagat gctaatatag aggaatcaga agtcagtaat 420

cttaagatca aagaagaaat tgaaaatgga aaatcagttt atgatattga ctttgtggac 480

ctgaaaaata aaaatgaagt tgattatcaa attgatgcag aaactggaaa aatcattgaa 540

cgttctagag atcatatgaa tgattaa 567

geqID 43

ttgaatcgta aaaaaaggta tcgtctcact gtaaaaaagc aaaatgctag catacctaga 60

cgattaaatc ttctattttt tattattgtt ttattattta ctgttctaat tcttcgttta 120

gagcaaatgc agattgggca acaatcattt tacatgaaaa aactgacagc tcttacgagt 180

tatacagtga aagaatcaaa agcgagaggt cagatttttg atgctaaggg agtagtctta 240

gttgaaaatg atgaacgtcc tactgtggcg ttttctagag gaaacaatat ttcatctcag 300

tctatcaaag agttagcgaa taaattatca cattacatta ccttaaccga agttgcttca 360

agcgatagag caaagagaga ttattattta gcagataaag ccaactacaa aaaagttgtt 420

gaaagtttac cagattctaa acgttacgat aaatttggta accatctagc cgaatcaacc 480

gtatatgcaa atgcagttgc tgcagttcca gtgagcgcta ttaactattc tgaagatgag 540

ttaaaagtgg ttgccttatt taaccaaatg aatgcgacac caacgtttgg ttcagtgaaa 600

ttaagtacag gcgaattaag tgatgatcaa atcaagaaac tggatgctga taagaaggaa 660

ctgttaggaa tttctgtaac aagtaattgg catcgtcgta aaaaaggaac ttctttatcg 720

gatatattag gtactatttc tacagaaaag gcaggtttac caagagaaga agttaaaaaa 780

tatttgaaaa aaggctattc actgaatgac cgagtgggaa cttcttacct tgaaaaacaa 840

tatgaagatg acttacaagg aatcagacaa attcgcaaag tagttgttaa taaaaaaggt 900

aaagttgttt cagataatat tacgcaagaa ggtaagtcag gaagaaatct taaattaacg 960

attgatctta attaccaaaa caaagtagaa agtatcttga aacaatacta tggcagtgaa 1020

ttatctagtg gacgcgctag tttttccgaa gggatgtatg cagttgctat agaaccttca 1080

actggtaaag tgttagcaat ggctggtctg aaaaatgatc atgggaattt agttgatgac 1140

agcctaggca ctattgctaa gaatttcacg ccaggatctg ttgtgaaagg tgcgacgcta 1200

tcatcaggat gggaaaataa agttcttaga ggaaatgaag tgctttatga ccaagaaatt 1260

gctaatatac ggtcatggtt tacacgaggt ttgactccaa tatctgcggc acaagcgcta 1320

gagtattcat ctaatactta tatggttcaa gtagcacttc gtttaatggg acaagactat 1380

aatacaggag atgctttaac tgatcgaggc tatcaagaag caatggctaa actaaggaaa 1440

acttacggcg aatatggttt aggggtttct acaggattag atttacctga atcagaaggt 1500

tatgtacctg gaaaatacag cttaggaaca actctaatgg aatcgttcgg tcagtatgat 1560

gcctatacac caatgcaact tggtcagtat atctcaacta ttgcgaataa tgggaatcgt 1620

ttagcacctc acgtggtttc agatatctat gaagggaatg attctaataa gttcgctcaa 1680

ttggttcgtt caatcactcc taaaacacta aataagatag ctatctcaga tcaagagtta 1740

gccattattc aagaaggttt ttataacgtt gtcaatagtg gaagtggcta tgcaactggt 1800

acgtcaatga gggggaatgt gacaaccatt agtggtaaaa ctggtaccgc tgaaacattt 1860

gctaaaaatg taaatggaca aacagtttct acctacaact taaacgctat tgcctacgat 1920

actaatcgta aaatagcagt agcggtaatg tatccgcatg ttacaactga tacaacaaaa 1980

tcccatcaat tagttgcacg agatatgatt gatcaatata tttcacagtt cacaggacaa 2040

taa 2043

SeqID 44

atgacggttt ttcctaaaca ttttctatgg ggaggtgcag ttgctgccaa tcaggtagag 60

ggtgcattta gaacagatgg caaaggttta tctgtacaag atgtacttcc aaatggtggt 120

ttaggcgatt tcaccgccaa acctactcca gacaatttga agctagaagc aattgatttt 180

tatcataact ataaaaatga tataaaactg tttgcagaga tgggctttaa agtttttaga 240

acctctattg cctggtctcg aatttttcca aatggtgatg atagtgctcc aaacgaagct 300

ggcttacaat tttatgataa tttatttgat gagttgctta aatataatat cgagcccttg 360

gttaccttat ctcattatga aacaccgctt catcttgcaa aaacttataa tggttgggct 420

gatagacgtt tgatagcatt ttttgagaaa tttgctcaaa cagtcatgga gcgttataaa 480

gataaggtga aatattggtt gacttttaat gaagtgaatt ctattttaca tatgcctttc 540

accagtggtg ctattatgac cgacaaatca caactaagtc ctcaggagct ttatcaagct 600

attcatcatg aattagtagc atcggctaga gtgacaaaat tagggcgttc tatcaatcct 660

aattttaaaa ttggctgtat gattctggct atgccagctt atcctatgac atctgatcca 720

agagatgtcc tggctgcaag acaatttgaa caacataatc tgctattttc agatatacat 780

gtcagaggta aatatcccac ttatattcag tcctatttca aaaataatgg tattaaaatt 840

aaatttgaag aaggagatga ggaagtattg gcacaaaata cagtagattt tctatcgttt 900

agttactata tgagcgtgac acaagcttat gactttgaaa attatcaaag tggtcaggga 960

aatatccttg gaggcctaac taatcctcat ttaacaactt cagaatgggg atggcaaatc 1020

gatcccatcg ggctacgcct agtcttaaat cagtattatg aacgttatca gattccgttg 1080

tttattgtag aaaatggatt gggagcaaaa gatcaattga tagaaacact agacggtgat 1140

tatactgtcg aagatgatta tcgtattgat tatatgaatc aacatttagt tcaagttgct 1200

aaagctattg aagatggtgt tgaaataatg ggatatacat cttgggggtg tattgattgt 1260

gtatccatgt ctacagctca actaagtaaa cgttatggtc taatatatgt tgatcgaaat 1320

gatgatggaa cagggagtct acaacgttac aagaagaagt catttggttg gtaccaaaaa 1380

gtgattaaga caaatggcca atcattgttt gaacatcata acagataa 1428

SeqID 45

atggctactt ttcaaattaa agaaaaaatg ttttctctag gcggtaaatt tactattaca 60

gaccagactg ggcttccctg ttatcacgtt gaaggaagtt tgttccctct acctaagact 120

tttaaagtct ttgacgaaga ggggcatctg attagtcaga ttgagaagaa ggttttaagt 180

tttttaccaa agtttaatgt gacattagcc aatgggaatc atttcacgat taagaaagat 240

ttctcatttt tgaaacctca ttacaccatt gaagaccttg atatggaagt taagggaaat 300

ttttgggata tagatttcca actcttaaaa gacaatcaag ttatagcaaa catatctcaa 360

cagtggtttc gtatgacttc aacttatcag gtagaagttt acaatgaaac ttacaacgat 420

ttgacaattt cgcttgtcat agcgatcgac tatgtcaaag agttagaaaa aaatgcatca 480

aactaa 486

SeqID 46

atgaaaaaaa taacaacttt aatcttagct agtagcttat tactagttgc aacgacatcg 60

gttaaagctg atgataactt tgaaatgcca acgcgttatg ttaaaatgag tgaaaaatca 120

aaagcatttt atcaaagact acaagaaaaa caacgtaagg cacatactac tgtgaagact 180

tttaataatt cagaaataag gcatcaacta cctcttaaac aagaaaaggc tagaaatgat 240

atctacaatt taggcattct tatttctcag gagtctaaag ggttcatcca acgtattgat 300

aatgcctatt ctttggaaaa tgtctcagat attgttaatg aagctcaggc tttgtataaa 360

cgtaactatg atttatttga aaaaatcaaa tctacacgtg ataaggttca agtcttactt 420

gcatcgcatc aagataatac agacttaaaa aacttttatg ctgagttaga tgatatgtat 480

gaacatgttt atctcaatga aagtagagtg gaggcgataa acagaaatat ccaaaaatat 540

aattag 546

SeqID 47

atgaaattaa aaaaattctt tgaggatttg ctggctaaac tagaatatag acccattcaa 60

gtttttatgc gtcattttca aagtgcagag atggacttat cagccatagc agttgcttat 120

tatctattag tgacagcttt tccattacta gttattgctg ctaatatttt tccttatttt 180

catattaatg tgtcggattt attgtcactt atgcaaaaaa atcttccgaa aaatatttat 240

gaacctgcct cacgcttagc agtagatgct ttttcgaaac cgtctaccgg cattttggga 300

ttcgcttcat taacagcttt ttggacaatg tctaaaagtt tgacctctct tcaaaaagcc 360

attaataaag catacggtgt tgaccaacac agagactttg tgattagtcg tttagtgggt 420

gtggggactg ggctgatcat cctattttta ctgacttttg ttttaatttt ttcaactttt 480

tcaaaaccag ttttacagat aattgtaaac atgtatgatt tgggagatac tctaacggcg 540

tggcttttaa atcttgcaca accagttaca tttttgacta tatttttagg tattggaatt 600

ttatacttta ttttacctaa cgcacgaatt agaaaagtac gttatgtcat tccagggaca 660

ttattttcaa cgtttgttat tggttttttt agtaatttaa ttagccaata cgtcttaaat 720

agggttgaga aaatggtaga tattaaaacg tttggttcgg tcgttatctt tatcttaatg 780

ctatggttta ttttcttagc ccacattatg attttaggtg caatactgaa tgccagcgtt 840

caagaaattg cgacaggaaa aattgaatca aggcgtggag atattatgtc tcttatccaa 900

aaatccaaag aagaaaaata a 921

SeqID 48

atgaaattac tttatactga tattaatcat gatatgacag aaatcttagt caatcaggcc 60

gcgcatgctg ctgaagctgg atggcgtatt ttttatattg ctccaaactc tttatctttt 120

gaaaaagagc gtgcagtatt agagaacctt cctcaagaag cctcttttgc tattacaatt 180

actcgctttg ctcaattggc acgttatttt actttaaacc agcctaatca aaaagaaagt 240

ttaaacgata ttggattagc aatgatcttt tatcgtgctt tagcaagttt tgaagatgga 300

caattaaaag tttttggacg attgaagcaa gatgcttcct ttatcagtca attggtagac 360

ctatacaaag aattgcagac agcaaatctg agtattttag atttaaaata tttacattct 420

cctgaaaaat ttgaagattt attagctatt tttctagtgg tttcagatct tttaagagaa 480

ggagaatacg acaatcaatc taaaattgcc ttttttacag aacaagtaag gtcaggccag 540

ctagatgtgg atttaaagaa cactatcctt atagtcgatg gttttacccg tttctcagct 600

gaagaagaag ctttaattaa aagtctgagt tcacgttgtc aagagattat catcggagct 660

tatgctagcc aaaaagcgta taaggctaac tttactaatg gaaatattta ttctgcaggg 720

gttgattttt taagatatct agcaacgaca tttcaaacaa aaccagaatt tatcttgtct 780

aagtgggaaa gtaagtctgg ttttgaaatg atttcgaaaa atatcgaagg taaacatgat 840

tttacaaata gtagtcacat tttagacgac actgctaaag attgtataac aatttgggag 900

tgcattaatc aaaaagatga agtggaacat gtggctagag ctatacgtca gaaactttat 960

cagggatatc gctacaaaga tattttagtt ttactaggag atgtagatag ttataaacta 1020

caattaagca agatttttga gcagtatgat attccttatt atttcggtaa agctgaaaca 1080

atggctgcgc accctttagt tcattttatg gattccttgt ctagaataaa acgttatcga 1140

tttagagctg aagatgtttt aaatcttttt aagacaggta tttatggaga gattagtcaa 1200

gatgatttag attattttga ggcttatatc tcatatgcag acattaaagg tcctaagaag 1260

ttctttacag attttgttgt gggggctaaa aaatttgatt tagggcgtct taacactatt 1320

cgtcagtccc tccttgctcc actggaaagt tttgttaaaa cgaaaaaaca agacggtata 1380

aaaacactca accaattcat gtttttcctt actcaggtag gtttgagtga caatttatcg 1440

cgattagttg gtcaaatgtc tgaaaatgaa caagaaaaac atcaagaagt ttggaaaca 1500

tttacagata ttttagagca gtttcaaaca atatttggtc aagaaaaatt aaatttagat 1560

gaatttttat cgttattaaa ttcaggtatg atgcaagccg agtatcgtat ggtccctgct 1620

actgttgatg tggttactgt taagtcttac gacttggtag aaccacatag taatcaattt 1680

gtctatgccc taggcatgac acaatcacat tttccaaaga ttgctcaaaa caaatcttta 1740

atatctgata ttgaacgtca gcttataaat gatgccaatg acactgatgg tcattttgac 1800

attatgacgc gagaaaattt gaagaaaaac cattttgctg ctctctctct ttttaatgct 1860

gctaaacaag cattagtgtt gactatacca caattattga atgagtctga agatcagatg 1920

tcaccatatc ttatcgaact aagagatata ggtgttcctt ttaatcataa aggacgccaa 1980

tcgttaaaag aagaagctga taatattggg aattacaaag ctttattatc gcgtgttgtt 2040

gacttgtatc gctctgctat tgacaaagag atgactaaag aagaacaaac tttttggtca 2100

gttgctgttc gttatttgcg tcgtcaatta acttctaaag ggattgagat accaataatt 2160

acagatagtc ttgatactgt gacagtttca tcagatgtga tgaccagacg cttcccagaa 2220

gatgacccac ttaaattatc ctcatctgct ttgacaactt tttataataa tcaatataag 2280

tattttttac aatatgtgtt aggtttggaa gaacaagact caattcatcc agatatgcgg 2340

caccatggaa cgtaccttca tcgtgttttt gaaattctga tgaaaaacca agggatagaa 2400

tcttttgaag agaaactcaa ctctgcaatt aataaaacca atcaagaaga tgttttcaaa 2460

tccttgtatt cagaagatgc agaaagtcgt tattctttag aaattttaga agatatcgcg 2520

cgtgcaactg caaccatatt acgacaagat agtcagatga ctgttgaaag tgaagaagaa 2580

cgatttgaac taatgattga taatactata aaaattaacg gtattattga ccgtatcgat 2640

cgtttatctg atggttcttt gggtgtcgtg gactataaat caagcgctca aaaattcgat 2700

atccaaaaat tttataacgg tctaagtcca caattagtta cctacattga tgctatcagt 2760

cgtgacaagg aggttgaaca aaaaccaccg atttttggag ctatgtattt acatatgcaa 2820

gaacctaaac aagacttgtc taaaattaaa aatttagatg atttagtaac aaaaaatcat 2880

caagctttaa cttataaagg attattttca gaagctgaaa aagaattttt agcaaatggg 2940

aaataccatc tcaaagattc cctttattca gaggctgaaa ttgctatttt acaagcacat 3000

aaccaattgc tttacaaaaa agcatctgag actattaagt caggtaagtt tttgatcaat 3060

ccatatacag aagatgctaa aacagttgac ggtgatcagt ttaagagtat tacggggttc 3120

gaggcggata ggcatatggc gcgtgccaga gcgctttata aattacctgc taaggaaaaa 3180

cgtcaaggtt tcttaacatt aatgcagcaa gaggaggaaaatgatgacct ttaa 3234

SeqID 49

atgagcgaaa ctaaagttat ggctttgcgc gaagcgatta atgtcgctat gagcgaagaa 60

atgcgcaaag atgaaaaagt atttttaatg ggtgaagatg taggtgtata tggaggagac 120

ttcggtacat cagttggtat gttagaagag tttggtgcta agcgcgttcg cgacacacca 180

atctcagagg ctgcgattgc aggttcagct atcggtgctg cacaaacagg tttgcgtcct 240

atcgttgatt tgacattcat ggatttcgta acaatcgcaa tggatgctat cgttaaccaa 300

ggtgctaaaa cgaattatat gtttggtgga ggtctatcaa ctccagttac tttccgtgtg 360

gcatcagggt caggtattgg ttcggctgca caacactcac aatcattaga agcttggtta 420

acacacattc caggtctaaa agttgttgca cctggtactg ttaatgagtc aaaagcactt 480

ttgaaatctt ctattcttga caataaccca gttatcttct tagagccaaa agctctctat 540

ggtaagaaag aagaagttaa catggatcct gatttctata ttccacttgg taaaggggat 600

attaagcgtg agggtactga cttaacaatt gtttcttatg gccgtatgct tgagcgtgtt 660

atgcaagctg ctgaagaagt tgctgaggaa ggtattaacg ttgaggttgt tgatccacgt 720

acacttattc cgcttgataa agaattaatt atcgattctg ttaagaaaac tggtaaattg 780

attttggtta acgatgctta taaaactggt ggtttcactg gtgaaattgc aactatggtt 840

gctgaaagtg aagcatttga ttaccttgat catcctatcg tgcgtcttgc aagtgaagat 900

gttccagtac cgtattcacg tgtacttgaa caaggaatct tacctgatgt agcaaaaatt 960

aaagatgcta tctacaaagt agttaacaaa ggtaaataa 999

SeqID 50

atggcttttg atgtaattat gccaaaactt ggggttgaca tgcaagaagg cgaaatcctt 60

gagtggaaaa aaaatgaagg tgacaccgtt aatgaaggtg atgtccttct tgaaatcatg 120

tctgataaaa ccaatatgga aattgaagca gaagacactg gtgtactttt aaaaattgta 180

catcaggcag gagatgttgt tcccgtcact gaagtgattg cctatatcgg agaagaaggt 240

gaagaagtag gtacgtcatc accttcagct gatgcaacta tcacagctga agatggtcaa 300

tcagtttctg gacctgctgc tccttcacaa gagacagttg cagcagcaac tcctaaagaa 360

gaacttgcag ctgatgagta cgatatcgta gtcgtaggtg gtggacctgc aggttattac 420

gcagcaatcc gcggtgctca acttggagga aaaattgcca ttgttgagaa aactgaattt 480

ggtggtacgt gtttgaatgt tggttgtatc cctacaaaaa catatcttaa aaatgctgaa 540

atcctagatg gtttgaaagt agcagctgga cgtggtatca accttgcttc aactaactat 600

gcaattgata tggataagac tgttgccttt aaaaattctg tagttaagac actgactggc 660

ggtgtacgtg gtctcttgaa agcgaacaaa gttgaaattt tcaatggact tggacaagta 720

aaccctgata agtctgttgt tattggcgat aaagttatca aaggtcgtaa cgtcgttctt 780

gcgactggtt ctaaggtatc tcgtattaat attccaggta ttgaatcacc attagtatta 840

acatcagatg atattcttga tttacgtgaa attcctaagt cacttgctgt tatgggtggt 900

ggtgtcgtag gtattgagct tggtcttgta tgggcttcat atggtgttga cgtaactgtt 960

attgaaatgg ctgatcgcat cattccagca atggataaag aagtatcgct agaacttcaa 1020

aaaatccttg ctaagaaggg aatgaagatt aaaacatcag ttggtgtttc tgaaattgtt 1080

gaagccaata atcaattaac gcttaaatta aacaacggcg aagaagttgt tgctgataaa 1140

gctcttcttt caattggacg tgttccacaa atgaatggtc ttgaaaatct tgagcctgaa 1200

cttgagatgg aacgtggacg tattaaagtt aatgcttatc aagaaacttc aattccaggt 1260

atctatgcac caggtgatgt taatggaact cgtatgttag ctcatgctgc ataccgtatg 1320

ggtgaagtag ctgctgaaaa cgcactcggc ggtaataagc gtaaagcaca tctagatttc 1380

actccagcag ctgtttatac tcatcctgaa gtagcaatgg tggggatgac agaagagcaa 1440

gcacgtgaac aatatggtga catattagtt ggtaaaaata gctttacagg caatggtcgt 1500

gctatcgctt caaacgaagc acatggtttt gtaaaagtaa ttgctgaacc taagtataaa 1560

gagattcttg gtgttcacat tattggtcca gctgcagcgg aattgattaa tgaagcttct 1620

acgattatgg aaaatgagtt gactgtctat gatgtagcac aatctatcca tggtcaccca 1680

acattctctg aagttatgta cgaagctttc ttagatgttc tcggtgaagc tattcacaac 1740

cctccaaaac gcaaataa 1758

SeqID 51

atgggaaaat attttggtac agatggtgtt cgcggtgaag ctaatgttga actgacgcca 60

gaattggctt ttaaattagg acgttttgga ggatatgttc taagtcaaca cgaaactgat 120

cgtccgagag tttttgtagc tcgtgatact cgtatttctg gtgaaatgct ggaatctgct 180

ttgattgctg gtctactttc agtaggtatt gaggtttata aacttggtgt tttagccact 240

cctggagtgt cttaccttgt ccgtacagaa aaagctagtg caggggttat gatttcagca 300

agccacaatc cagcacttga taatggtatt aagttctttg gtagtgatgg ttttaaactt 360

gatgatgatc gtgaattaga aattgaagct ttacttgatg cgaaggaaga cactttacca 420

cgtccatcag cacaaggttt aggtacttta gtagattatc ctgaaggact tcggaaatat 480

gagaagttta tggaatcaac tggtattgat ttagaaggta tgaaggttgc gttggataca 540

gcaaatggag cagcaacagc ctcagctcgg aatatctttt tagacttgaa tgctgatata 600

agtgttattg gtgatcaacc agatggtttg aatatcaatg atggtgttgg ttcgacacat 660

cctgagcaat tacaaagttt agttagagaa aatggttccg atatcggtct agcttttgat 720

ggtgatagtg atcgtttgat cgctgttgat gaaaatggag agattgtaga tggtgataaa 780

attatgttta tcattgggaa atatctttct gataaagggc agcttgctca gaatacaatc 840

gtaacaacgg ttatgtcaaa tctcggcttt cataaagcgc tcgaccgtga aggtattcat 900

aaagcgatta ccgctgtagg agaccgctac gtggttgaag aaatgcgtaa atcaggatat 960

aatcttggcg gggagcaatc aggccacgtt attatcatgg attacaatac tacaggggat 1020

ggacaattaa cagccatcca attgactaag gtcatgaaag aaactggtaa gaaattatca 1080

gagttagcta gtgaagtgac gatttatcct caaaaattag taaatattcg tgttgaaaat 1140

aacatgaaag ataaagcgat ggaagttcct gcaattgcgg aaattattgc taaaatggaa 1200

gaagagatgg atggcaatgg tcgtattcta gtacgtccta gtggtactga gcctcttctt 1260

agagttatgg cagaagcgcc aacaaatgaa gcagttgatt attatgttga tactatcgca 1320

gatgtcgttc gtacagaaat tggtttagat taa 1353

SeqID 52

atgaaaggtc aaaaaattat tgctctagct ggtcttgtcc tgtcatgcca ttttgctctt 60

acggcatgtc atactcaaga acacaaaaat tctcatcata ttaaaacaaa gcaggttgct 120

aagaaaaaag ctaataaaaa gaaagtctct gttaaggaaa gtcataaaaa acgcaagggt 180

gttgcagggg ttgactttcc aactgatgat ggctttttac tgactaaaga ttcgaagatt 240

ttatctcatc ccgattctgg aatcgttgta gcacatggaa atcattctca ttttattttt 300

tactctgatt taaaaggttc aaaatttagc tatttaattc ctaatgcaaa tgcaaaaact 360

aataaaaagc aagcggtaag aaactttaaa gcaggggcgg tagctgttaa tacattaaat 420

gatggctatg tttttaatcc cgcagatatt gtttcagaag atgctaatgg ctacgtggtc 480

agacatggtg atcatttcca ctatattcca aaagcaagtt tatctcagca aaagcaagta 540

caagcaagta gagctgtttc acgtttagga aatcaaaata atagccatta cagagttaat 600

agttctaaaa ttgcagggct tcaccaccca acaagtgatg gtttcttatt caatggacaa 660

ggaataaaag gcactacccc aacaggtatt ttagtagaac atcataatca tctgcatttt 720

attagttttg ctgatttaag aaaaggggga tggggatcaa ttgctgaccg ttaccaacca 780

caaaagaaag ttgattctaa aaaacagtca ccatcaagta aaaagccaag aactgaaaat 840

actttaccta aggacatcaa agataagcta gcttaccttg ctcgtgaatt acatttagat 900

atttcacgta tcagagtcct taaaacacta aatggtgaaa ttggatttga atacccacat 960

gatgatcata cacatgttat tatggccaaa gatattgatc taagtaagcc aattccaaat 1020

ccacatcatg atgacgaaga tcatcacaaa ggtcatcacc atgatgagtc cgaccataag 1080

catgaagaac acgagcacac taagtcaaat aaattatctg atgaagatca aaagaaatta 1140

atttatttag cagagaagct tggtttaaat cctaatcaaa ttgaagtatt aacgtcagag 1200

gatgggagta tcatctttaa atatccacat gatgaccact cacacactat agcaagtaag 1260

gatattgaaa ttgggaagcc tatcccagat ggacaccacg atcactctca tgcaaaagat 1320

aaagttggta tggcaactct aaaacaaatt ggttttgatg atgagattat tcaggatatt 1380

ctgcatgcag atgctccaac accattccca tccaatgaaa ctaatcctga aaaaatgcgt 1440

cagtggcttg caactgttac taagattaac attggacaaa gaaccaatcc attccagcgt 1500

tttggtctat cattgatgcc taatattgaa gttctaggaa ttgggtttac tccgattaat 1560

gatatgacgc cagttttgca attcaaaaaa cttaaacagc tttggatgac aaatactggt 1620

attacagatt attctttcct tgataagatg ccactattag aaggattgga tatttcacaa 1680

aacggtatta aggatttatc tttccttaca aaatataaac aattaagtct tattgctgca 1740

gctaataatg gcattacttc gctaaaacca ttggctgaat taccaaacct tcaattctta 1800

gttttgagtc ataacaatat ctctgacttg acaccgttat caaacttgac aaaactccaa 1860

gaattgcacc ttgatcataa taatgtgaag aatttaagtg cactttctgg taaaaaagat 1920

ttgaaagttc tagatttatc aaacaataaa tccgcagact tatcgactct aaaaacaact 1980

agtcttgaga cgcttctttt aaatgagacg aatacaagca atctaagttt cttaaagcaa 2040

aatcctaagg tatctaattt aacaattaat aatgcgaaat tagcatcatt agatggtatt 2100

gaagagagcg atgaaattgt taaagtagaa gctgaaggga accaaattaa gtcactagta 2160

ttgaaaaata aacaaggttc gttaaaattc ttgaatgtga ctaataatca gttaacatca 2220

cttgaaggtg ttaataatta tacttcactt gagaccttaa gtgtttctaa aaataaactt 2280

gaatctttag atattaaaac acctaacaaa acagttacaa atcttgattt tagccataac 2340

aatgttccaa catcacaatt gaaattgaat gaaaaaaata ttcctgaagc agtagctaag 2400

aattttccag cagttgtaga aggttcaatg gttggaaatg gtagtcttgc tgaaaaagca 2460

gctatggctt ctaaagagga caaacaagtt tcagataata ctaatcacca aaaaaacact 2520

gaaaaatctg ctcaagcgaa tgctgatagt aaaaaagaaa accctaaaac acatgatgaa 2580

catcatgacc atgaagaaac agatcatgca catgtaggtc atcatcacca ttaa 2634

SeqID 53

atgaataaac gcgtaaaaat cgttgcaaca cttggtcctg cggtagaatt ccgtggtggt 60

aagaagtttg gtgagtctgg atactggggt gaaagccttg acgtagaagc ttcagcagaa 120

aaaattgctc aattgattaa agaaggtgct aacgttttcc gtttcaactt ctcacatgga 180

gatcatgctg agcaaggagc tcgtatggct actgttcgta aagcagaaga gattgcagga 240

caaaaagttg gcttcctcct tgatactaaa ggacctgaaa ttcgtacaga actttttgaa 300

gatggttcag atttccattc atatacaaca ggtacaaaat tacgtgttgc tactaagcaa 360

ggtatcaaat caactccaga agtgattgca ttgaatgttg ctggtggact tgacatcttt 420

gatgacgttg aagttggtaa gcaaatcctt gttgatgatg gtaaactagg tcttactgtg 480

tttgcaaaag ataaagacac tcgtgaattt gaagtagttg ttgagaatga tggccttatt 540

ggtaaacaaa aaggtgtaaa catcccttat actaaaattc ctttcccagc acttgcagaa 600

cgcgataatg ctgatatccg ttttggactt gagcaaggac ttaactttat tgctatctca 660

tttgtacgta ctgctaaaga tgttaatgaa gttcgtgcta tttgtgaaga aactggcaat 720

ggacatgtta agttgtttgc taaaattgaa aatcaacaag gtatcgataa tattgatgag 780

attatcgaag cagcagatgg tattatgatt gctcgtggtg atatgggtat cgaagttcca 840

tttgaaatgg ttccagttta ccaaaaaatg atcattacta aagttaatgc agctggtaaa 900

gcagttatta cagcaacaaa tatgcttgaa acaatgactg ataaaccacg tgcgactcgt 960

tcagaagtat ctgatgtctt caatgctgtt attgatggta ctgatgctac aatgctttca 1020

ggtgagtcag ctaatggtaa atacccagtt gagtcagttc gtacaatggc tactattgat 1080

aaaaatgctc aaacattact caatgagtat ggtcgcttag actcatctgc attcccacgt 1140

aataacaaaa ctgatgttat tgcatctgcg gttaaagatg caacacactc aatggatatc 1200

aaacttgttg tgacaattac tgaaacaggt aatacagctc gtgccatttc taaattccgt 1260

ccagatgcag acattttggc tgttacattt gatgaaaaag tacaacgttc attgatgatt 1320

aactggggtg ttatccctgt ccttgcagac aaaccagcat ctacagatga tatgtttgag 1380

gttgcagaac gtgtagcact tgaagcagga cttgttgaat caggcgataa tatcgttatc 1440

gttgcaggtg ttcctgtagg tacaggtgga actaacacaa tgcgtgttcg tactgttaaa 1500

taa 1503

SeqID 54

atgactgcaa ctaaacaaca taaaaaagtt atcctcgttg gtgatggtgc cgtaggttct 60

tcttacgctt ttgcacttgt taaccaaggt attgcgcaag agttaggtat cattgaaatc 120

ccagctttat ttgataaagc tgttggtgat gctgaagatc tttcacatgc ccttgcattt 180

acatcaccta aaaaaatcta cgcagctact tatgcagact gcgcagatgc tgaccttgtt 240

gtcattactg ctggcgcacc tcaaaaacca ggtgaaactc gacttgacct tgttggtaaa 300

aacttagcaa ttaacaaatc aatcgtaact caagttgttg aatcaggttt caacggtatc 360

ttcttagtag cggctaaccc tgtagatgta cttacatatt caacttggaa attctcaggt 420

ttccctaagg aacgcgttat tggttcaggt acttcacttg actctgcacg tttccgtcaa 480

gctttagcag ataaaattgg tgttgatgct cgttcagttc acgcatacat catgggtgaa 540

cacggtgact cagaatttgc cgtttggtca catgctaacg ttgctggtgt ccaacttgaa 600

caatggttac aagaaaatcg cgatattgat gaacaaggac ttgttgattt gtttatttca 660

gttcgtgacg ctgcatactc aatcatcaac aaaaaaggtg ctacatatta cggtattgct 720

gtcgcacttg ctcgtattac taaagctatc cttgatgatg aaaatgcagt tcttccatta 780

tctgtatatc aagaaggcca atacggtgat gttaaagatg tctttatcgg tcagcctgca 840

atcgtaggtg cacatggtat cgttcgtcca gttaatatcc cattaaatga tgctgaactt 900

caaaaaatgc aagcctcagc tgaacaatta aaagatatta ttgacgaagc ttggaaaaac 960

ccagaattcc aagaagcatc aaaaaactaa 990

SeqID 55

atgcaagata aaaatttagt agatgttaat ctaactagtg aaatgaaaac gagttttatc 60

gattacgcca tgagtgtcat tgttgctcgt gcacttccag atgttagaga tggtttaaaa 120

cctgttcatc gtcgtatttt atatggtatg aatgaactag gtgtgacacc tgataaacct 180

cataagaaat cagcacgtat tactggtgat gttatgggta aataccatcc acatggtgat 240

tcatctattt acgaagcaat ggtgcgtatg gcacaatggt ggtcatatcg tcatatgctc 300

gttgatggtc atggaaactt tggttcaatg gatggggatg gtgctgccgc acagcgttat 360

acagaagcac gtatgagtaa aattgctctt gagatgcttc gtgatatcaa taaaaataca 420

gttgattttc aagataacta tgatggcagt gaacgtgaac ctcttgtttt accagcgcgt 480

ttccctaatt tattagtcaa tggtgcaact ggtattgctg taggtatggc aacaaatatt 540

ccaccacata atctaggtga gtctatagat gctgtcaagt tggttatgga taaccctgat 600

gttactacac gggagttaat ggaagttatt cctggtccag attttcctac tggtgccttg 660

gtgatgggac gttcaggtat tcaccgtgcc tatgaaactg gaaagggatc aattgtctta 720

cgttcacgga ctgaaattga aacaacttca aatgggaaag agcgtattgt tgtcacagag 780

tttccatacg gtgttaataa aacaaaagta cacgagcata ttgtacgttt agcacaagaa 840

aagcgtattg aaggtattac tgctgttcgt gatgaatcaa gtcgtgaagg cgttcgtttt 900

gttattgaag ttcgtcgtga tgcttcagca aatgtgattt taaataatct tttcaaatta 960

actagcttgc agacaaattt tagcttcaat atgcttgcta ttgaaaaagg tgttccgaaa 1020

attttgtcat tacgacaaat cattgataac tatattgaac accaaaagga agttattgtc 1080

cgtcgtactc agtttgacaa agctaaagca gaagctcgtg ctcatatctt agaaggttta 1140

cttgtggcgc ttgatcactt ggatgaagtt atcacgatta ttcgtaatag tgagacagac 1200

accattgctc aagcagagtt gatgtcacgt tttgaattat cggagcgtca gagtcaagca 1260

attttagata tgcgtcttcg tcgtttaact ggattagaac gcgataaaat ccaatcagaa 1320

tacaatgact tgctagcttt aattgctgat ttagcagata tcttagctaa gcctgaacgt 1380

gttgttacca ttataaaaga agagatggat gaggttaagc gtaagtatgc tgatgcccgt 1440

cgtacagaat taatgattgg tgaagtttta tcacttgaag atgaagacct tattgaggaa 1500

gaagatgttc ttattactct ctcaaacaag ggatatatca agcgtctcgc tcaagatgag 1560

tttagagcac aaaaacgtgg aggacgcggt attcaaggga ctggagttaa taacgatgat 1620

tttgttcgtg aattggtttc aacaagtact cacgatactg tgcttttctt tacaaattta 1680

gggcgtgtgt atcgtttgaa agcatatgaa attccagaat atggacgtac agctaaaggc 1740

ctccctatcg ttaacctttt aaaacttgac gaaggtgaaa caattcaaac aattattaat 1800

gctagaaaag aagatgtcgc taataaatat ttctttttca caactcagca aggtatagtg 1860

aaacgtacaa gtgtttctga atttagtaac attcgtcaaa atggacttcg tgctattaat 1920

ttgaaagaga atgacgagtt gattaacgtc ctcttgattg acgaaaatga agatgttatt 1980

atcgggacac gtacaggcta ctctgttcgc ttcaaagtga acgctgtacg taatatggga 2040

cgcacagcga ctggggtacg aggcgttaat cttcgtgaag gtgataaagt tgtgggagct 2100

tctcgtatcg ttaatggaca agaagtactg atcatcactg aaaaaggtta tggcaagaga 2160

acagaagctt cggaatatcc tacaaagggt cgtggtggta aaggaataaa aaccgctaat 2220

attactgcta aaaatgggcc attagcacgc ttagtgacga ttaatggaaa tgaggacatc 2280

atggtcatta cagatacagg cgttattatt cgaaccaatg ttgctaatat ttctcaaact 2340

ggtcgttcta ctatgggtgt taaagtgatg agacttgatc aagaagctaa aatcgttact 2400

gttgcgctag tggaacaaga aattgaagat aagtctaata tagaggatac aaaagaatag 2460

SeqID 56

atgacaatct ttgatgaaag agagttaaaa gaacgattta ctcatgaaaa tagggttagt 60

ttttatgagt ttgtggctaa atatgatgct caaatggttc ctgtgatgaa agcaaagggc 120

tatcgatgta ttcattcaat ggagcgtaca gtggttttta cttttgggga attcacgatt 180

aggcgacgtc ggtggcaaaa aggagaacat tgggtggtgc cagttgatga aaagctagga 240

ctgaaaaaga atgttcgcta ctctttagaa ttcatgtatc aaattgctag tttagcaacc 300

atgatgcctt atgaaaaagt gattaaggtt gttcagatga tgtattgtat tgtgattacc 360

aaacctactg ttgtgaaggc cgttaagatt agtcgtgaat tgcttaaaga aaaggaagcg 420

tatcgttttt ttgatgaaga tataccagta gataaagaac cagttgatat gatttatctt 480

gaaggagatg gagtcatggt caaagctcga gaagaaggat tagataatcg caatgttgat 540

ttgtctcatt tcgtggttca tacgggtagt cagaaagtag gaagcaatcg ctttgtctta 600

caaaataaaa aagagtttgt gtcccttgat aatcgtcaaa cgcgtcaaaa gattttggac 660

tacctttaca atcattttta cattgcccca aacaccttgc ttattaccaa ttcagatggt 720

ggccatggct ataccccata tgtttttaaa gagattgcga aagcactcaa agtgaagcaa 780

cacgaacact tttgggatag gtatcatgtc aatgaaaaga tcaaaagttt ttttaaactc 840

tatccagtgg aactgatgac tggcgctttt cagagcatta aaaagcatga taaagaaaag 900

cttagaactg ttttggatac gacagaagca ttgatactga tggaagaaga aatggaaggg 960

tttaaccagt tcaaacgaaa gttgttaaac aattttcaat atactaaatc agctgaattg 1020

agaggtttca gtcgtgcagg aataggtgtt atggagtcac aacatcgaaa aattacctat 1080

cggatgaaaa agcgcgggat gtattggaca attcagggag ctgagacgat gagtcaatta 1140

attgtcctat cgtatgaagg acagctaaga gatctcttct ttggttcttg gcgtgaagac 1200

tatcagaaat atcaagagct agaaaacctt agtgcgggaa aaatcaaaca tgaacagaat 1260

aaaatcaata agagatatga tctccagaca cttggtcggc ttaggtacgg taggcatcgt 1320

aatttatag 1329

SeqID 57

atggcatatt tatcaaaatt atccgatttg gatccatcgt tgatggatgc ggattctgaa 60

caaatttata ttcctaaagt cttgtttgag cataatgact ttaaaggctt gacctacaaa 120

gagattttat tatattcttt tttgttaaat cgtttaagag agccattaga ttttattcaa 180

aaaggctatg atgataatga agatacctat gttcacttta aggtcgaaga tttatgtgaa 240

ctactcaacc agagtaagac aaccgttatt tccttgaaga aaaggttagc tcaatatggt 300

ttgatagaag aagtgaaagc aggtagtcac cagccgaatc gtatttattt aacagataaa 360

ttagttccat atattaaggg gtaa 384

SeqID 58

atgacagata atcgctttgc ccaattaaaa gaaaactttg aaaagggatc tcctaaaagg 60

cgagttccaa cgtctcgccc aatcgcagct caaaaagcgc ctgagagcta taacaaaaag 120

ggacggtatc cattttcgct ccaccaagat gtgcgttatg ataaattaga agcattagta 180

gcttatcatg gagctaagtc tgcatcagat tatctggaga ggttgattgt tcaggaatgg 240

gaaaagatgc agcggaagct taagaacaaa gaaaaataa 279

SeqID 59

atgtttagtt ggttggaagc tctttattac actctgatac aactggcaaa agtgaaccgg 60

ttgaatgctc ttttcttagt tagcgttgtg ggttatcttt gttaccaggg aataaaactc 120

gtcagaaaaa ccataagaaa cttttttcag ctgatgaagg gtttcatagg tgatagagag 180

aacatcaaga aatgcatcaa aaacaagaaa gaggcactag tccattcttg gaaacatcgt 240

caagatattg attggaaatc aactggaaaa gataagagta aacagttatg gaatcttatg 300

aagcgacttg cgacagttgc tccttcattt ttgttcttat tattgggaaa tgttctcttt 360

cgtctcattt atcaactgcc ttttgtaaag caagacagaa agcgatttga caaggaaatg 420

aagcccttgc tctacttcaa gaactatcgt agttttgtgt tcatgggaat aggtttcagt 480

ttcatagcgt ttattctcac aaactatttt gtgacggttt taagggctgc tattcgtttt 540

ctatatttct caatcatgac gttaagagat aatagccaag tcgttagctt taacgttgat 600

agtttgctca tccagaattt attcaatgct agggtatttg tgatagctcc cattctagca 660

gtgccaatct ttctcattgg tttagtcgta gcttggcggt ctgcttgggt taactttgaa 720

cagtaccgtg attataatca taatgaagaa ggggatgatc gctttgcgac tgtcaaagaa 780

atccaccagc aatataagaa agttcctaat aaaacggaaa cttatccagg tgaaggaggt 840

gtgcccgttc ttcatgaaac aagaaagaat ttgacaggct taacgcttaa atctcaaatg 900

ctttggcaaa accgtacctt tagtcgctat ttaacgaatg cggaaaggat tttagggctc 960

ttatcgacgc cttcaggaga ttattacatc gatgatagca ccacaaactt gatcaccatg 1020

gggattactc ggtcaggtaa gggagaagct catattgccc ctattattga tattaatagc 1080

cgtgcggaaa ttcaaccgtc actgattatt gcagacccta aaggggaaca ttaccagtcc 1140

tcttataaaa ccatgcgtcg gcgtggctat gatgttaatg tcctttcttt ccaaaacatg 1200

gattggtcca tgtcctataa ccctttagct cttgcgattg cagcagctaa gaagggttac 1260

tatgaaatga cacagacaag ggttaatgcg gttgcagaag ccatttatcg taaaacgaaa 1320

cctggtagcg gtaatggcaa tgcaaaatac tgggaagata cctccatttc cctctttaat 1380

gccattgcta tggccttaat ggaccgtgct aatgaaaccg tcaggaatgg tgaaaccgat 1440

gcttgggata ccgttacagt tcgtaacatt gccaagtttt tgactgactt gggttctgaa 1500

gaagtctttg tcaatgattt tggagagatt gttgagaatc ctgataagaa ccaacaagtg 1560

aagaagaaat ctaaaatcac ggtttacttt gataacttgc gtaaaatcaa tcaagaacaa 1620

ttttccaaat ttagagatat ggctgattta aactttaggt cttctgactt cgcttcagaa 1680

gaaactaagg gaaatgtctt ctctagcatg atgtcaggta ttaacttatt cttgcaagat 1740

aatattgcta aactaacctc taaaaactct attgacctag aatcggttgg tttcccacgt 1800

cgcttgtcta tcaagtttcg ttctagttcc aatgtcgcta tgcgtaacga atacactcat 1860

aagacggcta aggttaccat tactagtcaa gctgtttggg gtaaaaccac taaacaagtt 1920

atccacgtag atgctgcaac agctctgatt gatggtgaag gctatctaac ctatgtgatt 1980

gaaccccagc ttcctgatca attcttggta acaattgact ttaatcacga aaacaatggt 2040

ggttcagcta ttcgtcacaa aactttccaa ttctcagctg agaaagtcta taagaaacgt 2100

ggtaacgtta ttacgttgga tgactacacg aaaaaaccag ttttggatca tatcaaagtt 2160

actgttctca acaagcaaga tgataacctt ctccagaaag aagatattga cctgatttat 2220

tcagataatc ctaaagtgat ttacttggta acacctccaa ataggactga atataatagt 2280

attgtatctc tgtttttgga tcaattgttt aatgccaatt atgagttagc tatgtcaaat 2340

ggtcgcaagt gtgtcaatcg aattcttcat atcctcgatg aattcacaaa cattccagct 2400

attcctcaca tggataccaa gatttccatt ggtcttggtc aaaatattct ctactatctc 2460

tggattcaga acttgaaaca gttagtcagt gaatatggcg agaatacagc ggaaaccatt 2520

cgggagaact gttctttgaa agtttatatc aaatctactg ccccagcgac caacgagtac 2580

ttcagtaaag agttggggac tcggaccatt acacgtcgca gaaggtcaag taatattcta 2640

gatgaagcta atccaaatgt ttccattgaa aatcctagac aggaactctt aacaccgaca 2700

cagctctcga aactccaaga aggggaagcg gttattttgc gtggtgttaa aggtcgagac 2760

aatgcaggtc ggaaaatcac aacggatccg attttcttgc atgagaaaac gagccttcct 2820

tatcgctaca tgttcttaca agaagaattt gaccagtcga tggctttggc agatattcca 2880

gtggaaagtg ggcataggga ccttgacctg caagatatag cagtaggggc acaaagcact 2940

tttaataaga ttattgattg gcggatggct ctaactgacc gtatgagaac aaatgggaag 3000

atacctcaat tagcatcaag aaaacaaacg attaaagctc taagtcaatc tcaatttact 3060

tctccagcag acctaacaca agctgtgatt gcagaggtat ttgatgagga agatgatgac 3120

gatcttttct ttgtggatga tgtcatgtaa 3150

SeqID 60

atgaattcta acacaaaagg tcacggattt ttccgcaagt caaaagcata cggcttagta 60

tgtgctattg cattagcagg tgcatttaca ttagctacta gtcaagtgtc tgctgatcaa 120

gttacaactc aagcaacaac tcaaacagta acgcaaaatc aagcagaaac agtaacatca 180

actcaacttg ataaagcagt agctacagct aaaaaagcag ctgtagctgt tacaaccaca 240

cctgcagtta atcatgcgac aactactgat gcacaagctg atttagctaa tcaaacacaa 300

gctgttaaag atgttactgc aaaagcacaa gctaatacac aagctattaa agatgctact 360

gctgaaaatg caaaaattga tgctgaaaac aaagcagagg cagagcgtgt tgcaaaagaa 420

aacaaggaag gtcaagcagc cgtagatgca cgtaacaaag caggtcaagc agccgtagat 480

gcacgtaata aagcgaaaca gcaagcgcaa gacgatcaaa aagcaaaaat tgatgctgaa 540

aacaaagcag agtctcaacg tgtaagtcag ttaaatgcac aaaataaagc aaaaattgac 600

gcagaaaata aagatgcgca agctaaagca aatgcgacta atgcacaatt acaaaaagat 660

tatcaagcta aattagcgga aatcaaatca gttgaagctt ataatgcagg tgtacgtcaa 720

cgtaataaag atgcacaagc taaagcagat gcgactaacg cacagttaca aaaagactat 780

caagctaaat tagcacttta taatcaagct ctaaaagcta aagcagaagc agataaacag 840

tctattaata atgttgcttt tgatatcaaa gctcaagcta aaggtgttga taacgctgaa 900

tatggaaact caatcatgac tgcaaaaact aaacctgacg gaagtttcga gtttaaccat 960

gatatgatcg atggtgtgaa gacaatcggc tatgggaaat tgacaggtaa agttaatcat 1020

cattatgttg ctaacaagga tggctctgtg acagcatttg ttgattctgt cactctttac 1080

aagtacgagt atcgtaatgt tgctcaaaat gctgctgtta accaaaatat tgtatttaga 1140

gttttaacaa aagatggtcg tcctattttt gaaaaagctc ataatggtaa caaaactttt 1200

gcagaaactt taaacaaaac tttacaactc aatcttaaat atgagcttaa accacatgct 1260

tccagcggta acgtcgaagt ctttaagatt catgatgact gggtacatga cacacatggg 1320

tctgctttag tgtcttatgt taataataat gatgctgttc ctaatgtggt catcccagaa 1380

cggccaactc caccaaagcc agtgaaagtt acacctgaag cagaaaaacc agtacctgaa 1440

aagccagttg agcctaaatt ggtaacgcct acattaaaaa cttatactcc agtcaaattt 1500

attccgcgag aatacaaacc agaaccaatc acccctgaga cgtttacccc tgagaaattt 1560

actccagctc aaccaaaagt gaaaccacat gtgtctattc ctgaaaagat taactactca 1620

gttagtgttc atcctgtttt agttccagct gctaatcctt caaaagctgt cattgatgaa 1680

gcaggtcaat ctgttaatgg taaaaccgta ttaccaaatg caacattaga ctatgttgct 1740

aaacaaaact ttagtcaata caaaggtatt aaagcttctg cagaagcgat cgcaaaaggt 1800

tttgcatttg tagatcaacc aaatgaagcg ttagctgaat tgactgttaa gtctatcaaa 1860

gcatctaatg gtgatgatgt atcaagcttg ttagaaatgc gtcatgtttt atcaaaagat 1920

actttagacc aaaaacttca atctcttatt aaagaggcag gaattagtcc agttggtgag 1980

ttttacatgt ggactgcaaa agatccacaa gctttttata aagcttatgt tcaaaaagga 2040

ctagatatca cttataatct atcctttaaa atcaaagcta actttactaa aggtcaaatc 2100

aaaaatggtg ttgcacagat tgattttggg aatggatata caggtaatat tgtagtcaat 2160

gatgttactg ttccagaagt acataaggat atacttgata aagaggatgg taaatcaatt 2220

aataatagta cggttaagtt aggtgatgaa gtgacctaca aacttgaagg atgggttgta 2280

ccagcaaacc gtggttacga tctttttgaa tacaaatttg tggatcaatt acaacacaca 2340

catgatcttt acttacgtga taaagtggtc gctaaagttg atgtgacatt aaaagatggt 2400

acagtcatta aaaaagggac taatttagga gagtacacag aaacagttta caacaaaacg 2460

acaggtcatt atgagcttgc ctttaaaaaa gagtttttag ctaaggtttc tcgtgaatca 2520

gaatttggtg cagatgattt tattgtagtt aaacgtatta aggcaggtga tgtttacaat 2580

actgctgatt tatatgttaa tggatataaa gttaagtcag aagcagttgt gactcatact 2640

actgagaaat caaaaccagt tgaaccacaa aaagcaactc caaaagctcc agctaaagga 2700

ctgccatcaa ctggtgaagc tagtatgacg ccacttactg caattggagc aattatctta 2760

tcagctctag gcctcgcagg ctttaaaaag cgtcaaaaat aa 2802

SeqID 61

atgaaacaaa taaaaattat cacaggactt acagtcgcaa cactatccgc agtggtaggg 60

aatgtatacg cagaagatat cacaccgaca gcaccagtta atgagccaca agtttcaagc 120

gaaaccgcaa aaacgcctca agttacagaa agtcaagtta atagcgcaaa agttactgcc 180

gatcaagcaa taagtgatgt taataaccaa caaattgtag ttgatgaggc tcaaaagcag 240

aaagatcaat cacaacaaaa tcttgtaaaa gccacatcaa cagtaactga agctgaaaaa 300

gtcgctgctg aggcaacacc tgaagttgtt aaagaagcta ttaaagctgt cactgaagca 360

aaggaagctg ttacggatgc tgaagctaat gtagtcgatg cacaaaagac ggaacaaaaa 420

gctaatcaag aagtacaatc gcaagctaag actgttgatg aaaatgttaa agttgttgct 480

gataaagaat ccgaagtgaa acaagcagag ggtgttgtaa ctacagctca agaagctatt 540

gatagcaaaa ccgctaacac taatgcatca gaagcagaaa aagcagtgac tgagaagcaa 600

acaaagcttg aaactgctga aacaaatctt acagaggcac aaaaacaaga tgccaaaatc 660

gctgaagaaa aacgacttgc agaacaagaa gttgttaata aacaattagc tgtgacagat 720

acacaaactc ttttgaagaa gcttgttact gagattaata acgagaaagt aagcactagt 780

ttagaaaacc aagcctattt taaccaacgt gacggttcat gggcgggcta ttatggcaat 840

tatacttttg ccgctactgg ctgtgtacca agtagtttag caatggtatt tacggagtta 900

gctagacgcc aaattactcc aactgaaatc gccaattatc tttggaacaa ttcaaatgag 960

tttaataaaa actatggtgg aacaagtggc aaagggttag tacaagctac taaacatttc 1020

ggttttgtac caactcattt agcatctcaa tctgctattg tggaagcctt acaggcagga 1080

caccatgtcc tagcagctgt ccaacaagat aaattttctc cttggggtat caattacagc 1140

catgagattg tcttacgtgg ttactcaaat ggcaatactt atgtttacga tccttataat 1200

cgagctaaca ttgggtggta ccctgttgct aatttatgga atgaacaaag tagagacgca 1260

attgacacat ctagtgtagg cgtaccattt ttcaaaatta caacacaaaa aatggcccaa 1320

ttagaagctc agaaagcaca agttcaatct tctctaaata cagctaaaaa tcagttagct 1380

aaaacacaag acgtattaag aacactagaa gcaacacctc ttaaaacacc agaagcacaa 1440

gctaagctta atcaagccaa agaagctcta gctcttgcac aagcagacta tactaaagcg 1500

caagaagctg ttaagttagc tagtcaagac ttagctgtta aagaagaaac acttaaaaat 1560

gctcaggctg atttattaac gaaacaaaca gccttgaaag atgctcaaac tgttctcgtg 1620

gctagtcaag ttaaattagc tgatcttaaa gcaactttag ctactgttga aaataacgtt 1680

aagaaagctc aagctacttt aacagatgcc aaagcaattg ttggtcaaaa acaagcaaaa 1740

ttacttgctt tacaaaatgc ccctaaaatt ctagcagatg ctcaagctaa acttgtaact 1800

gctaaaaatg atttagctaa taaaatggct attttagatg aagcagtcgc aaaactaaaa 1860

tccttacaag ccgttcaagc cgaagcccaa aaacaatacc atgttgtttt tgaagcctat 1920

aaagcagtac gagatgccaa agaacaagct aaacttgcag aaagctataa ccatattatc 1980

gcacgaggcg gtgaggctat cccagttgtt gacgaaacag ataaaataac tggatatgta 2040

gatggtagcc agaaagcagt tgctaatgaa gtgactcttg ccttaactag taacggtgca 2100

ccccttgaaa gtccagttaa taaagaaaac caaaacgtaa caaaatcatc tcaagcttta 2160

ccacatactg gtgaagctgg attatccatt ttatcagtat taggtgtagg tcttatctca 2220

actttagggc ttactagttt gaaaaaacgt cgcccacatt aa 2262

SeqID 62

gtgagattaa cccacgtgag tttgtggatg ccatcaaaga gaagttggcc gctcaaattg 60

cagttgttaa ggaaaggagt gattgagttg gaagatgaac tgaagacccc ttatcttgat 120

caatacacag acaatttaac ggctaaagtc actaaaaaat cggatgacta ccaagtttat 180

ggacgaaaca aggaggttca atccgtcatc atctctcttc tcagacgaac caaaaataac 240

cctattttag taggagaagc aggggttggt aaatcagcca ttgtggaagg tataacgctt 300

gctatcttac gtggccaagt tcctgaacct ttaaaaggtt taacggttcg ttccctggag 360

ttgtctagtt tgatgagtga agatgacgaa ggctttattg ctaagtttaa gaagattatt 420

gaagagatgg ttgctacacg tggtcataat cttctctttg tagatgaatt tcatacgatt 480

attggtgcgg gtagccagaa tggtcaagcc cttgatgcag gaaatgtgat taaacctgtc 540

ttagcacgtg gtgatattca gctgattgga gcaaccacct tagatgagtt ccatgagtat 600

attgaaacag atagagcctt ggaacgtcgg atgaagcctg ttatggttga agagccaacg 660

atttcacaag ctattaccat tattgaacaa gctaaagtca tttatgagaa gtttcatggg 720

attcaaattt cctcagatgc tgtgcatcaa gcaatccgtt tatctgttcg ctatttgaca 780

gatcgattct tgccggataa ggcctttgat ttgatcgatg aagcggcgac gattgcttca 840

gttgaaggga aaagtaaggt gacagaaaaa gatattgctc aagttttaaa agataaaacg 900

ggaattccag tcactactat cctaaaggga gatcaagagc ggttagaggg tttcaaagaa 960

aggctgatga atcgagtcaa aggtcaagaa gatgccattg aggccgttgt agatgcggta 1020

acgattgctc aagctggttt acaaaatgaa aaaaggccgc ttgcatcatt ccttttcctc 1080

ggaccaactg gcgttgggaa aacagaatta gccaaagcaa ttgcagaagc actttttgat 1140

gatgaagctg ccatgattcg ttttgatatg tctgagtaca aacaaaaaga agatgtgact 1200

aaactcatcg gcaatcgtgc gacaagaata aaaggacaat tgactgaagg agtaaaacag 1260

aagccttatt gtgtcctgtt actagatgag attgaaaaag cacacagtga ggtaatggat 1320

cttttcttgc aagtgctaga tgatggtcgt ttaacagata gttcgggtcg tttgattagc 1380

tttaaaaaca ccattgtgat tatgaccacc aatattggcg ctaaaaaaat catcaataag 1440

tgggagttga aaggaaactt taaagattta accgatcgag atcggaaaca atttgaaaag 1500

tcgatggaca gtgagcttca aaatgagttt cgtccagaat ttctcaatag gattgaaaat 1560

aagctcatct tcaacctttt agaacgtgat gtgattgaaa aaattgcaga aaaaaatcta 1620

tctgagattg cggataggat gaagcgccaa aatttaacac tgtcatatga gcctagtctg 1680

attcagtatc tctctgatgt tggtactgat gtgaaaaatg gcgcacgtcc cttagaacga 1740

ctgatgaaac gaaaagtgct ggcacccatt tctgtaaaaa gcttacagtt agataagtca 1800

aagcaaggct ataacgttca tctctgggtt gagggacggg ctccagacgg caatcatcgt 1860

caagaacaac gtcaaattca catggagata gagggagaaa gagataactt ttttagctga 1920

SeqID 63

atggcagaag aaaatgcaca acagccatct ttacgtggaa aaagccgtcg agaacgtgtt 60

gaatttgctc gttcccgtga tattttagat gtggcccatg aattgaatat ggaacttttt 120

cgagatggca aaaattatcg ttggaaagag cacgattcta tggtaattac acctgcaact 180

aatcagtggt actggttttc acaacgtcaa ggaggtgatg tgattgcgct tgtagagacg 240

ataaaagaaa tcggcttcaa tcaagcattt gaatacctga atgaaggtac atttaaagaa 300

tttactgtgg tcaatcaagt aaaagaacct ttttcttatt atttagaacc ttacgaacag 360

ctttttgttg aagcaagacg ttatttgaaa gagaatcgtg gcttatcaga tgacactatt 420

gatttttttt atgataaagg agtacttgct caagccaatg ccaaggtagg tgatatgatt 480

gagcctgttt tggtgtttaa aaacttggat aagaacggcc aagtggttgg cgctgctcta 540

caaggcttag tagctgcccc cgataaatat ttcggtcggg gttacctcaa acaaatcatg 600

aaaaattccc aaccttacaa cggcatgcat gttgatattg gcacaccaaa ccgcctagtc 660

tttgcggaaa gtagtattga tcttatgagt tactatgaaa tccacaaaga tagcttatca 720

gatgttcgtt tagtttccct agaaggcttg aaaacaggaa caataggaag gcatctcatc 780

caattaagag ctgaaatgga acgtcgtccc ctctcctcca gttggactga tgaaatactt 840

gctcagggat tagatgaggc tgtaaagcaa ggctatttca aagatggtaa gaacagtcat 900

ctccttacgc tagctgtaga taatgatgta aaaggtaaac aattgattga agagttaaaa 960

gataagagca tcccagtcat tgatgccact cctcctaaag cagaaggtca atctaaaatg 1020

gattggaatg cctatttgca ggaaaccaag gctacttttt ctactgaaaa atatcaagaa 1080

aagattgatc acctaatatc agatgttatt ttaggtgatg aaacttatta tctttggcat 1140

gatgatgagt tggtaaattt aggagcagga gactctatta tacaggcgtt tcatcatcag 1200

ctggaagaca gacgttatgt tattaatcag gcagaacttt acgttgaaga gtccagtaat 1260

gatggggcta ctggctatct atcaattgaa ggacatgtat tagataagga tggtattagt 1320

gactatttat ctgatcaagc tttaacagat gcggaaaaag tagcattctt agaaacttta 1380

cagacagaac taccagatat ttgggacgag atagtcaatc attatgacaa ggtctttgaa 1440

gaagttgtcg ttaaatatgg actcagagaa aaacatgcgg acataattca ggaacaagag 1500

ttggatttag accctttagt tgttccagaa gcaaaagaaa agtcccttga aatgaatcaa 1560

gagactaaca ctggcggcga actatttaat cgcaattcca gttttttagg agaagattct 1620

ccggggacag caccgcagcc cgttgagcca actgctcaac ctgattttcc taccaatgtt 1680

cgcttacatt ttaccactga tgatggaaat atgtcaaata aagcatttag gaaaaacatg 1740

cgaaccttga atttatatgc taatacgatg agagattcgg cacaatggta cttatcagaa 1800

atagccgata ctacaatgag ttatgtttat aaaactcctc atgaagaggg agtacaggtt 1860

cttagtgtgc attttggtaa aaagaattgg atgcacttaa caggagtgac accagtatat 1920

gaaaattggg tagattcact ttctgaacaa tttattgatg atattgctaa tagtaaggga 1980

cattttaaaa atctaaagtt tgcactaggg actcctgata agttaaaggt tcttaactta 2040

cttcctgaaa ttattgaatc agataccttt gtatttaatg atttatcatc tgttcaaaag 2100

ttaaataatc ttgatttatc acaggcgctt aatcctgaag atagcgattt acttttgcta 2160

tttagagatg aggggcttca tcaagtgcct gcttcgttga tgaggataaa gggcgattta 2220

gaagaaagat tatctcatat agatagtggg acagtgttag gagtttaccg agaaagaaat 2280

ggccaacttg agcaagtttc tgtcaatgag gagtatgtca aagatagtgg tcaagaaatg 2340

ctatctattt tacagaataa gcactatgaa gaggctcttg atagtggtca agaaatggtt 2400

caaacggatg gtttttcagc cgaagatttc acaaaagttt tggatgctgt ctatcatgtt 2460

ggtgttccag atgatttagc acgtgttcca gaaggagtct tacctgtttg gcaaaaatat 2520

cttgaggtat cagaagagaa tcaatgggac ttagaacaaa tgattgatta tgcggataag 2580

aacagtcttt tagtaaaaga ttctgccttc tataaagagt ggaaagagga tatgatctat 2640

aaaaatgact atcatgttcg cttacaattt gctgagaatt gggataatgg cgttgaactg 2700

cctttccgta cagaacaatt gattgattac aaaacgtttg tcacaggact ttatgaagct 2760

aatcaagctc atcatcaacg cagacaagaa agtcagcttc cttatacaaa aacagagttt 2820

gacatttatg ctcctggcgg acagctcatt aaagataatg tccattatgc tattggagat 2880

gaaacaagac ctgtttcaca actcatgggg ttaggttatc gtcgcctacc tggctatcag 2940

gaattagctg tgatagataa cagtattctt tctcagctgg aaaataagga gctaaatcaa 3000

gaaattgctt ctgaagctaa tgaacattcc ttaaattcac aagaaatacc taaagaagat 3060

aactatccaa gagaagcttt cacttctcct aaacaagaca tcaaaaaggg gctagctcaa 3120

cgagtagaag agattgtggc agaagatgct actaagattt tagtatcttc cattcctcaa 3180

gttcaagaga atttatcagt cgaaggaaac ttggtcggta ctccacaagc ggataatcgg 3240

atgctataca ctaacctaga agattttggc caagattatc aattagagct tgcagtttat 3300

agtccaaaac gggttgactt tttagaagat gtgcaggctc cgtggacctt ggccctaatt 3360

agaaaagaaa aaaagatagg ttatttagcg tatggtagtg attgggcaaa agaatttcag 3420

attgaagaag agctagaaca tttagctgca caaattggag acgaaaaagt accagaggga 3480

ctctataaac aagctgaagt agaggctttt atagctagtc atcagggaaa tgaaagcctt 3540

caggaaccaa tacctactat tgtagctgaa ccgtttgact ataccagtgc ctccgcctat 3600

gagatcagcg aacacgcttt ccaaaagatt cgtgagtaca cacaatcccc tgaggacttg 3660

cttgagtaca tggatttcat gagtaagttc ccacaacttt cacctcgaaa tgtcgctctt 3720

atccatgaac aatggcgtgg cgcaaatgca gtcgcaactt acgaacaatg gaaagccatg 3780

ggagaagctc taggtattaa accagacgat gttgtccaaa caaaagcaac ttatgttaat 3840

aagcggacag gagagacgaa ggaagtcgtt catcaagggt tatctgttaa gactggcgaa 3900

aaatcgaaaa ttacgctctt tagaccgcta atggttaaaa tgatcccagt tcttgatgag 3960

aatggtcaac agctgaaaaa tgataaaggt aatccaaagt ataagaagct atcagaagcg 4020

tcacttcaag agaaagcttt agtaaaagac ggtaaacttc cagttcgtca atttcaggag 4080

agagattcaa aaactggtca accaaggttt acgacttaca aagtttttga gttgtcacaa 4140

actactctga agccaggaag ctatcctaag gctatgccaa accgtcattt caactttaat 4200

gtggataaag tcaagactaa agaggtatta gaagggcttt gtgactatgc ggaaaagatt 4260

ggggttagcc taatgaaaga tgatgctcat gttttggata atgccaaagg agctttttat 4320

tcagaagaac agctgattct tattaatcct aataatacac ctggagaaaa aattgcaact 4380

accattcatg agttagcaca tgcaaccctt cataatccta agttggaaaa acagtataaa 4440

gagttgccga aaggacaaaa agaatttgaa gctgagatga ccagttactt gttgtcaaaa 4500

cactttggcc tagatacgtc tgaaaaggca atccattata tggcaagttg gacagataat 4560

ctaaaagcgt tagaggataa acaattggca gactctctga aacgagttca tcaaacggtt 4620

tcgaaaatgt tgaagcaagt tgaaaaatat acgaaccctc accagctagg aagggggaaa 4680

gaacacggtctaaattttcc taaagcccca accaaagggt ctagtcgtta a 4731

SeqID 64

atggatgtat cgtctagtcc gaatattaca tttatgctgc aatacacaga ggctaatcct 60

caatatgtgg actatactaa cagagaagag gctgtcaaaa ttgatgaaga attgtcctta 120

gaaacgaaca ggcaaatgat tgaaggatta actgaagacg agttgactcg tattcaggaa 180

gctgtccctg aaacgcagtt gaattttagg gaatacattg attatatgaa ccgctcgtat 240

gcaactgaag aacaatctaa agaactaaca gctatcttta ctcaagaagc agattatctt 300

cagaaactac gattaatcga tctaaaaaat aagttggaat cagcttatca aaatggttca 360

cttctctggc aaggagttat ttcgtttgat aatgcttttc ttgcggaaca gggattgtat 420

gatgttgcga ctggtcaagt tgatcaaaaa gcgattaagg cagtgatgcg tgatatgatg 480

ccaacactta tccagaaaga gggcctttct gattctgctt tttggtgggg gaatatccat 540

ctgaatacag ataatatcca tatccatttt gggctttctg aagttgaatc taaccgtgag 600

aaaatattct atcagccacg tggacgtatg gagtacaaag gtaacttctc tcagaaaacc 660

atcaaccggt ttaaaagtgg tgtgtatcat ggattgctga aagaagaaac aagatccaat 720

cttctcagaa aagaacagat tttagctaat ctcaaagcgg acttcataac atctatttac 780

cagaaggaca agattacttc ttcagctgaa aaaaattttt tggaacaagc ctacaatcat 840

ttgccgctaa ataagaagtg gcgctatggt tctaatgcca gagattttgc ggttagtaag 900

ttctttcttg atcgctattt agattcctat ttaaacaatg aaggtagtgc tgcctatcaa 960

gaatttttga aagagactag agattttctt cagacttatg aaggggttta ttcagctgaa 1020

aaaaataaaa tctatgaaaa actacgtaaa gttgatgggc aaacgatcag aacgcttgca 1080

gaatcaaaag gatatgattt agaacatcat ttggcacgtc gtgtaatgga tttaagagag 1140

cgtttagcca ataatatctt acgttcgttt agagaagctg caccccaaat tcaagacgtt 1200

cagctggaaa aaaatttaga gagtttttct gttttgaacc agaagaaaat tttagaacaa 1260

catcctgaag caagtgtggt aaaaagtcag aaagcttggc agaagttagg ctactttgtg 1320

aaggctggag agcagccact tgaaattata aggccagtct ataaatctta tgataagcat 1380

ggtaaaggta taggacggcc agaatttgta tcagatactg tttatgatat tagtcagcta 1440

acagaaaata ttcagctgaa aagtctaacc ttgaaagacc tctctctgtt ttcttctaac 1500

gagttaaaag agttagtaga tgctgctaag ttaaagacta atccaacaga gagagaacgc 1560

cgtgaattag gtacctatcg ttatgcgttg aaacttagca tattagaatc tagtcagaag 1620

gaattgcagg ttcgtcaaaa gctactagaa caggtacagc cactagcgtc tgatcaacca 1680

tttttagatt ttaagaaaca gttaatagct caggaattac aagctatagc gctacaattg 1740

actcctaatt acaagctatc agaagatgat aaagccttga aaaatcgatt gaagaggcag 1800

tttgaagata gtgttgcgct acctgtttca aaagctactc ctggtgccat acaacttcct 1860

attaggcaac tttggactga gctaggattg gttcatcaca ttcaagatga aaacattcta 1920

acgcttctga aagggacatc aacgacgaaa caagcttata tagaagaact tcagactcat 1980

atctctattt ttcagttgaa atatcagatt aacaatagaa acaagcagat aagccagtta 2040

tcggatgaag caacaataaa ggagatgagg atagctaatg ctaaaggttt ctctgagcta 2100

aaacgtctat atgatacatt acagccatca gatgatggtc aaaatcagat tagtcaagct 2160

gtttctaaac aattacaaga acgaaaagtt atcaaaaaag ctcaattaca acagacgcag 2220

agaagcggaa aaatcaatac agacttcatg cgacaattga cagcttctct taatcgttca 2280

caacaagcaa gtaaaaaagc attgatggaa cgtgcacgta gtgatgaacg tgaggaacaa 2340

gaagaacgta ggcaagctca acgttaa 2367

SeqID 65

atgaagaaga ataaatttct attagtcagc attgtattta tcatcatttt tgtggtacag 60

ccacaaaatt ttcaatcttt aaaaaatata tttactcaaa atgatatagc tagtcaattg 120

aatatatcaa gttcacctga agaaaaaaat gatggcttag gaacggccta tcaaacgcaa 180

aatgaagatt tgaaatctaa aagttttgat ggtcaacatc aagtcatagt ggttaatgaa 240

aaagcacaat ttacggcaga agagttgagc atgagaaatg gttcatggga aaaatacgat 300

aacctagatt ttttaaatcg tgttggggtc gcagaagcta tgttaggaaa agagttaatg 360

ccaaaagagg cacgtcagga catttcttca gtcaaaccaa ctggctggaa aaataaaaaa 420

attaccttca atggaaagca agattatctc tataatcgtt ctcacttaat tggatttcag 480

ttaagtggtg agaatgccaa tgtgaaaaat ctctttacag gaacacgtgc ccttaatgca 540

aactttaacg atgataaatc gtccatggtg tactatgaaa atgaggtcgc aaactacatt 600

aagaaaacaa atcatcatgt tcgttatcga gtgaccccac ttttcaaaaa tgtggagtta 660

gtagctcgtg gcgtacgtat agaagcacaa agtattgaag atgagaccat ctcatttgat 720

gtctatattt ttaatgggca accaggatat gacattgatt atttgacggg atcatctgaa 780

aaaataatga ttacaaaatg a 801

SeqID 66

gtgactaaag aaattaaaat tagatccatt ccagaaaaaa catgggcaca gctacatatg 60

atttcggaag aatatgagta tccgtctttt aatgaattta tgcttgctca attgcaacgg 120

attgtcgaaa atggtggtct tgatttatat gataataaat ttgcggaaac cttggcagtt 180

attaaagaac aacaagcaca aatattggat cagctcctaa aaaacgaaat caagttactg 240

gcttatcatg caaagcaaga tatagtggaa gagctaacaa ccgactggtt acgatttatg 300

gatgatgttg atgcgctggc tgcggaaaga ggagcaggag gacgttga 348

SeqID 67

ttgttatatt attttattta cttgataaaa gtaattggaa atggccttaa attatcactg 60

atttgtggtt taaattggct catcaaaata gtattcaaag gacaatttta tcttttctca 120

gctgtctttt gtggcttgtt gacctactat atgccacaag atattcaatt atttacagtg 180

agagttttag agctaatcat catgctaaaa gtgataatag atgttaccca tacagctctt 240

tcaagagatt ttaaacggat gaaaacgcct ctttttttag gagtgatgta cgtcttcttt 300

ctagctggta acagttatat taaagcacat cttttgacag aagttatggt taatcatctt 360

atttcgtttt ggcttatcag cctgtttttt gccactcttg taatagttat tcagcccaga 420

ttatttaagc attatctgtt aaaaaaagtc attgataagg aatacttggg tatccgaaaa 480

ttcacagact ctcttcctcc agaaatcaat ttgtacaagg atgcggacga agaagatgcg 540

gacaaacgga tgcgactgat caaccagaat gtcatcaaac acccctatca agaagttgtt 600

gaactgagct ttttgaatag agaagtgata acagctattg gctataaagc cgttccattt 660

gaaaaagaaa ctgaacgtac ttttatagac gatgatacca tctattatcc catttttacg 720

gttcaccctt tcagaaattt ggaagggaaa tcagattttt atcacatact aatgaaactt 780

aaactcagtc gaaaagcggc ctttacaaaa aatggtgagc gattattaat tagagatttt 840

taa 843

SeqID 68

atgattagaa atgaatttta taatcagtta atcaatagtg agccaatagg ttttattgat 60

cctttcactg acttaggaga atttgattct attcagatga agttcaaaca acctgttaga 120

aatctggtaa ataagtactc tggtaaacct tataatctta gttggcaaaa taagattgaa 180

cagatgagag tgctatatat taaatatcag aaaagcttga agctagaaga tgaagaacaa 240

gaggttcata accgagttaa aaataaaaag tctaaaaaat atgttcatga aatcgttaca 300

acatatttga agttaggatt caggtttaaa gaaattgaag caagggtatc cctattcaat 360

actcgtcttc gtcggaactg gaaaagaagc gactatgtga caactgataa ccctgaattc 420

tatttgaaga aggatctaca gaatggttat tgttctccaa attcatttct tcctcggagt 480

atgaaaataa actaa 495

SeqID 69

atgaacgaaa tcaaatgccc tcattgtgga acagcttttg ccatcaacga gtctgaatac 60

catcaattac tagaacaaat tcgtggagat gcttttgaca aagaagtaag tgaacggttg 120

gaaaaagaac gtctaatatt aggggagcaa gcaaaaaatc aattacagga agttgttgta 180

gaaaaagaca aggagatagc taaacttcag tacaaagtca aacaatttct tatagaaaaa 240

gacaatcttc tcaaagacaa tgagtaccaa ctcgctgagc aattaaatca aaaagacatg 300

atgcttcgcg accttgaaaa ccaaatcgat agactacgtt tagagcatga aaatagcttg 360

caagaggcgc taacaaaagt cgaacgagaa agagatgcaa tacaaaatca gttgcacatt 420

caagaaaaag aaaaagattt agctttagct tcagtaaaaa gtgattatga agtacaacta 480

aaggcagcca atgaacaagt agaattctat aaaaacttca aagctcaaca gtctactaaa 540

gcagtaggag aaagtttaga acattatgct gaaacagaat ttaataaagt gcgacatttg 600

gcctttccta atgcttattt tgagaaggac aatacattat caagtcgtgg ctcaaagggg 660

gactttatct atcgagaaaa ggatgaaaat gaccttgagt ttttaagtat catgtttgaa 720

atgaaaaatg agtctgatga tactatcaag aagcataaaa atgaagattt tttcaaagaa 780

ttagataaag atcgtcgtga aaaatcttgc gaatacgcag ttttagtaac tatgcttgaa 840

gcagacaatg actattataa tactggaatt gttgatgtta gtcacaaata ccctaaaatg 900

tacgttatac gtccacaatt ttttatccaa ttaattggta ttctaagaaa tgcagcactc 960

aataccttaa aatataaaca agagcttgct ttgatgaaag aacaaaatat tgacatcaca 1020

cattttgaag aagatttaga tattttcaaa aatgcatttg ctaaaaatta taattctgca 1080

agcaaaaatt tccagaaagc aatcgatgaa atagataaat ctattaaacg tatggaagct 1140

gttaaggctg ctttaacaac gtctgaaaat caactacgtc ttgcaaataa taaattagac 1200

gatgtttctg tcaagaaatt aacaagaaaa aatccaacaa tgaaagcaaa attcgatgct 1260

ctaaaagact aa 1272

SeqID 70

atgaatcatt ttgaactatt taagcttaaa aaagctggac taacaaatct taatatcaac 60

aatattatca actatctcaa aaagaatagt ttaacttctc tatctgttcg caatatggcc 120

gtagtatcaa aatgtaaaaa tcctactttc tttatagaaa attataaaca gctagacctt 180

aaaaaacttc gacaagaatt caaaaaattt ccagttctat cgattttgga ttctaactat 240

cctttagagt taaaagaaat atataatcca ccagttctac ttttttatca gggtaatatt 300

gaacttctat ctaaacctaa attagctgta gtgggggcaa gacaggcatc tcagataggt 360

tgtcagtctg ttaaaaagat tatcaaggag actaacaatc aatttgttat cgtaagtggt 420

ttagcgcgtg gcattgatac agcagcacat gttagtgctt taaaaaatgg cggcagtagt 480

atagctgtta tcgggagtgg tttagatgtt tattatccaa cggagaataa gaaacttcaa 540

gaatatatgt catataatca tctcgtatta tcagaatatt ttaccggaga acaacccttg 600

aaatttcatt tccccgaacg taaccgtatt attgcagggc tgtgtcaagg tattgtggtt 660

gccgaagcta agatgagatc tggaagttta attacctgtg aaagagcatt agaagaggga 720

cgagaagttt ttgccattcc cggaaatatt atcgatggca aatcagatgg atgccaccat 780

cttatccaag aaggagctaa atgcattatt tcaggaaaag atatcctttc tgaatatcag 840

tag 843

SeqID 71

atgactgaac gaacattcga agatattgaa cttgacttaa agttattcca aataaagctt 60

gataatgctg agaatagtaa aaggctctta caaaaattga aaaacgacgt catggagtta 120

caaatagagt tactagaatc attaaaactc ggtgatgcct atttaacaga atcagaagaa 180

ttagaagaga ataatgactt tattctaacg gtaaatagtg aaacactaag tttatcctat 240

gacaatagga taaacttagt ttctaaagag attatggatt atgaaaatgc attagataag 300

ttgtattatg aaaaacagag tttgatgcaa aaaagtaatg aaagaaaagg aggttaa 357

SeqID 72

ttgttcaata aaataggttt tagaacttgg aaatcaggaa agctttggct ttatatggga 60

gtgctaggat caactattat tttaggatca agtcctgtat ctgctatgga tagtgttgga 120

aatcaaagtc agggcaatgt tttagagcgt cgtcaacgtg atgcagaaaa cagaagccaa 180

ggcaatgttc tagagcgtcg tcaacgcgat gcagaaaaca gaagccaagg taatgttcta 240

gagcgtcgtc aacgtgatgc agaaaacaga agccaaggta atgttctaga gcgtcgtcaa 300

cgtgatgcag aaaacagaag ccaaggtaat gttctagagc gtcgtcaacg cgatgttgag 360

aataagagcc aaggcaatgt tttagagcgt cgtcaacgtg atgcggaaaa caagagccaa 420

ggcaatgttt tagagcgtcg tcaacgtgat gcagaaaaca gaagccaagg caatgtttta 480

gagcgtcgtc aacgcgatgt tgagaataag agccaaggca atgttttaga gcgtcgtcaa 540

cgtgatgcag aaaacagaag ccaaggtaat gttctagagc gtcgtcaacg cgatgttgag 600

aataagagcc aaggtaatgt tctagagcgt cgtcaacgcg atgttgagaa taagagccaa 660

ggcaatgttt tagagcgtcg tcaacgtgat gcagaaaaca gaagccaagg taatgttcta 720

gagcgtcgtc aacgcgatgt tgagaataag agccaaggta atgttctaga gcgtcgtcaa 780

cgcgatgttg agaataagag ccaaggcaat gttttagagc gtcgtcaacg tgatgcagaa 840

aacagaagcc aaggcaatgt tctagagcgt cgtcaacgcg atgcagaaaa cagaagccaa 900

ggtaatgttc tagagcgtcg tcaacgtgat gcggaaaaca agagccaagt aggtcaactt 960

atagggaaaa atccacttct ttcaaagtca attatatcta gagaaaataa tcactctagt 1020

caaggtgact ctaacaaaca gtcattctct aaaaaagtat ctcaggttac taatgtagct 1080

aatagaccga tgttaactaa taattctaga acaatttcag tgataaataa attacctaaa 1140

acaggtgatg atcaaaatgt catttttaaa cttgtaggtt ttggtttaat tttgttaaca 1200

agtcgctgcg gtttgagacg caatgaaaat taa 1233

SeqID 73

atgaaaagac taacttatta ttttaagggg tatatcaaag aaactatctt tggacccctt 60

ttcaaattat tagaagcctc ttttgaactt ttggtaccaa tcgttattgc aaaaatgatt 120

gacgagacca ttccacgagg ggatagaagt agtttactgt tgcaaattgg attgattttc 180

tttttggctg cggtgggtgt tgtagtagcg ataactgctc aatattattc ttcaaaagcc 240

gcagttggtt atacgagaca attgacagag gatctctacc aaaaagtcat gtcactgggg 300

aaaaaagaca gagatgaatt gggaactgct agtttgatta ctcgtttaac tgctgataca 360

tttcaaatcc aaactggttt aaatcaattt ttacgtttat ttttgagggc tccgattatt 420

gtttttggtg ccataattat ggctttttcc attagcccct cattgactat ttggttcttg 480

gtaatggttg tcacgttatt tatcattgtt tttgtaatgt ctcgactatt aaatcctatt 540

taccttaaaa tcagaacttc aaccgattat ttggtaaagt tgactaggca acaacttcaa 600

ggtgtacgag ttatccgtgc ctttaatcaa gtagatagag agagtgaagc atttaatgat 660

atcaattatc attatacgaa tttacaatta aaagcaggta ggctttctag tttagtaacg 720

cctctaacat ttctagttgt taatatcact ttggttgtga ttatttggcg tggtaattta 780

aatatagcta atcatctttt atcacaagga atgttggttg ctttaattaa ttacttattg 840

caaattcttg ttgaattgtt aaaaatgaca atgttggtga catcactgaa ccaaagttat 900

atcagtgcta agcgaattat agccgttttt gaaagaccgt ctgaaataat tgatgacaaa 960

cttgagccaa aatattcgaa taaggcttta gaagtacaag aaatggcatt ttcttatcca 1020

aattcttctg aaaaagcttt atctgatatt actttttcta tgaatgtagg agaaacttta 1080

gggataattg gtggaactgg ctctgggaaa tcaaccttgg ttaatttact gcttcatatt 1140

tataaagtgc aagaagggga tattgatata tatcatcagg gaaaaagtcc agatacaatt 1200

tcaaattggc gtaccctggt aagagttgtt cctcaaaatg ctcagttgtt taaaggaact 1260

attcgttcta acctttcttt gggacttggt aaagttagtg aggaaaaact ttggactgct 1320

ttagaaatag cacaagctag tgattttgta aaagaaaaag atggtcaact tgatgcccct 1380

gtagagagtt ttggtagaaa tttctctggt ggtcaaaggc aaaggttgac gattgcaaga 1440

gctttagttc aagataagat accatttttg attttagacg atgcaacatc tgcattggat 1500

tatttaacag aagctcgttt atttaaagcc ataactaaac atttcaacca aactaatctt 1560

attattgtat cacagagaat taatagtata caaaatgcag atagaatctt actccttgat 1620

aaggggaaac aagttggttt tgataatcat caatctttat tagctcataa caaagtttat 1680

aagtccattt accattcaca aaattttaag gaggaggagt aa 1722

SeqID 74

atgaaattta atgagcaaag taactcgcaa gcagcacttc taggcttgca acacctgtta 60

gctatgtatg caggatccat tcttgtacct atcatgattg ctagtgctct tggttataat 120

gctgagcaac taacttatct tattgcgaca gatattttta tgtgtgggat tgccacctta 180

ttacaattac aattaagtaa gcactttgga gtgggtcttc cagttgtatt agggtgtgcc 240

tttcagtctg tagctccttt atcaattatt ggtgcacaac aaggttccgg ctatatgttt 300

ggagctttaa ttgcttcagg aatatatgtc gttttagttg ctggcatttt ttctaaagtt 360

gcaaatttct ttcctccaat tgtaacagga tcagttatta ctacaattgg tttgacatta 420

ataccagttg cgatgggaaa tatgggtgat aatgcaaaag aaccgagctt acaatcttta 480

actttatcac tggtaacgat tggtgttgtt ttattaatta atattttcgc aaaaggcttt 540

ttgaagtcaa tttcaatcct tattggactc atatcaggta caattcttgc agcatttatg 600

ggcttagttg atgcttctgt ggtagcagaa gcaccacttg tacatattcc gaagccattt 660

tattttggag ctcctagatt tgaatttact tctattttaa tgatgtgtat tattgcaaca 720

gtttctatgg tagaatcaac aggtgtttac cttgcgcttt cagatattac aaacgataaa 780

ttagacagta agagacttcg taatggttac cgttcagaag gattggcagt attacttggc 840

ggcttattta atacctttcc atataccggt ttttctcaaa atgtgggact ggtacagata 900

tctgggatac gtacgcgtaa gccgatatat tttacagctc tcttcttagt tatacttggc 960

ttgttaccta aatttggcgc aatggctcaa atgattccaa gtccagttct tggtggtgct 1020

atgttagttt tatttggtat ggtagcactt caagggatga aaatgcttaa tcaggttgat 1080

tttgagcata atgagcataa ctttatcatt gcagccgtat caattgcagc aggagtaggt 1140

tttaatggga caaatctctt cattagtctt cctaatactt tacaaatgtt tttgacaaat 1200

ggtattgtta tctcaaccct gacagctgtt gttttaaata tcattttgaa tggattgcct 1260

aaaaaattaa tatga 1275

SeqID 75

atggctaata catacgattt aatttcacag cgtattgaag cacaacgaca gaaactcatc 60

gctatagata ttgtggccgt tgcgagctca ctaggactta acttgaaaca aggttcaggt 120

ggacatctct attgggatga acatgatagt tttcatattt acccccaaac taacaccttt 180

cgttggtggt caagaagcat ggggaccaat accattgatt tagtccaagt catccaagaa 240

gagctaacag gaaagaaacc tagctttcga gaaactgtca actttttaga aacagggcaa 300

tttgaatcgg ttacggtaac accagtcgtt agagaaccct ttaagcacta cctcgctcct 360

tatgaacatc acaattttga cttagggcga cagtatctta aagaagaacg agggctatct 420

gatgagacca ttgattttgc tttggcatca ggtagtatga gctctgcgac attgaaaaag 480

ggtgattatt ttgaacctgt tattatcttt aaaagttttg cggaagacgg ccgaatgatc 540

ggcggtagcc ttcaggggat cgttgaaaat aaagtccagc atcctgaacg tggccgtctc 600

aaacaaatca tgaagcattc agacggtcta gcaggttttc atttggacgt tggaacacct 660

aaacgtctcg tgttttcgga agctcctatt gaccttctct cttattatga attacataag 720

gagagtttac agaatgtccg cttagtcgct atggatgggg tcaaaaaagg ggtgattagt 780

cgctatacgg ctgatttatt gacagatggc cagtattctc aaaccatgcc cagagagtcg 840

attagaggag cgatagatgc cattaaccaa acgactagaa tccttaaaaa caatcccaat 900

atgattacca ttgccgttga taatgacgag gcaggccgaa actttattaa ggagttacag 960

gaagatggca ttccaattaa cgtagacctc ccaccacgga aagaacacca gagtaaaatg 1020

gattggaata attatctgaa acaaaagaaa ggattactaa aaatgccaca aacagaaggc 1080

acacaaaaag ccccagaaca agttctggag catgaaaaaa tggataggag ccaaattagt 1140

tcgggctctt tagaggacga ccctcaaggc agtgccaaac ctgtatccaa aagggatacc 1200

tttgagcaag ctgttaccag ccacccgact ttctcctatc ctttactaca gtttagcaca 1260

gaagaagcct ttgtgtcaaa cgttagagat ggctaccaca tcgcaagtga ggaggatatt 1320

cggaatctca attactatgc ccctagtctc caacaaacgg ctaactggta tcgggataat 1380

ctggcagacc gtcaagtgac ttatatgtta aaaggggata aggagataaa ggcgcttcag 1440

gtcagttttg ccaaggataa gtttgcccac ttaaccggta ttcgtccgat tggaaaaggg 1500

ttgtcagctg aaaaactgtt agatgatttt gcgaaaggtc gaggttccta tcctaatctg 1560

accttatcaa atggttttaa tgacaagata caagttttgc caatgattca agaactatct 1620

caatccaagt cttttgtctt tacagattta gaggaagttc aaaaaatgag aaacttaaag 1680

gccagtcatg cgattcaatc caataatcgg agtttagtcg tcgctttaaa aactattgat 1740

gatgtcacct ttccttcgtc cctcctaaga ggaaaaaaga atctaaatga tgatctgatt 1800

caaaaggcga aagaaaatga agttctaggt gtactcagtg agaaagatgg gaacatcacg 1860

gtgctgtccg ttaacgataa gtacatccaa gatggtgggc aagccttaaa agatatgatt 1920

aaaaatggcg aacttgaacc actccaaatg gagacgatac agcgacatgt tcctcatgag 1980

aatgcctatc ctaaggattc tgacggtgat ggcttaaccg atgatgaaga aatcgctcta 2040

ggcaccaatc cctttagttc tgatagtgat ggtgatggga ctccagataa tgtcgaaaag 2100

gcaaatggaa cagaccccac caatgcttct gataatgagg tgacaaggca acaagaagcc 2160

aataaacgtg acttcacttt gtcagaaatg attaaagcca aaaataccgc agcgctgaat 2220

caacacttac aagacggcat caaacagtat tttgatagtg atacctataa gcaatacctg 2280

gaagggatgg cccacttcaa taactactcg ccacgaaata ttcaattgat tatgtcacaa 2340

tttccagaag catcgatggt tgcgtctttc caagagtgga ggaagcgaaa tggttcggtt 2400

aaaaaaggtg agaaggccat ctatatccaa gcccctgttt ctgttatgaa aaaagatgag 2460

aatgggaaac ctatccttaa tcctgaaacg ggagaaaagg agaccatcac ttactttaaa 2520

cctgtccctg tctttgacat taaacaggtc tctccccaag aaggaaaaga actcaatctc 2580

cctaaagcca tgggaactat tccagaacaa ttggataaag aatactacca aaatgtctat 2640

cgtagcttaa gagatatttc tcagaataac aataaggttc ccattcgatt tagagaactt 2700

ggacaagaag atgggttcta tagtccacag acaaatgaaa ttgttattaa gaaagggatg 2760

tcttatgaac gaaccttgtc aacgctgatt catgaaatgg ctcattcgga attacacaac 2820

aaacaaagtt tgacggaacg ctttgatggt aaactaacaa gaagtaccaa agaacttcaa 2880

gcggaatcca ttgcttatgt tgtatccagt catctaggtt ttgataccag tcaagagtca 2940

tttccttatt tagcgtcttg gtcaaaagaa aaagacgggc ttgccaactt aacagcacaa 3000

ttggaaatcg tgcaagaaga agccaaaaac ttaatggaac ggattgatca acagttaagt 3060

caatatcaaa cagtaacact gaataaagaa acccaacaat tgactaaaca agaaatgaaa 3120

aagcagactc atccctttta tcaaagttta gcagccgcta aaacatcaag agcacaagtc 3180

accactcagg agaaggaagc aagcgtaaaa aaagacaatc gacctacgat gccgtag 3237

SeqID 76

atgaattcac aagaaacaaa aggtcacgga ttttttagaa aatcaaaagc atatggttta 60

gtatgtggga ttgcactagc aggtgcattt acattagcta ctagtcaagt gtctgctgat 120

caagttacaa ctcaagcaac aactcaaaca gtaacgcaaa atcaagcaga aacagtaaca 180

tcaactcaac ttgataaagc agtagataca gctaaaaaag cagctgtagc tgttacaacc 240

acaacagcag ttaatcatgc gacaactact gatgcacaag ctgatttagc taatcaaaca 300

caagctgtta aagatgttac tgcaaaagca caagctaata cacaagctat taaagatgct 360

actgctgaaa atgcaaaaat tgatgctgaa aacaaagcag aggcagagcg tgttgcaaaa 420

gctaataaag caggtcaagc agaagtagat gctcgtaata aagcaggtca agcagccgtt 480

gatgcacgta ataaagcaaa acagcaagcg caagacgatc aaaaagcaaa aattgatgct 540

gaaaacaaag cagagtctca acgtgtaagt cagttaaatg cacaaaataa agcaaaaatt 600

gacgcagaaa ataaagatgc gcaagctaaa gcagatgcga ctaatgcaca attacaaaaa 660

gactatcaaa caaagttagc aaacattaaa tctgttgaag cttataatgc aggtgtacgt 720

caacgtaata aagatgcaca agctaaagca gatgcgacta acgcacagtt acaaaaagac 780

tatcaagcta aattagcact ttataatcaa gctctaaaag ctaaagcaga agcagataaa 840

cagtctatta ataatgttgc ttttgacatt aaagcccaag ctaaaggtgt tgataacgct 900

gaatatggaa actcaatcat gactgcaaaa actaaacctg acggaagttt cgagtttaac 960

cacgatatga tcgatggtgt gaagacaatc ggctatggta agcttacagg taaagttaat 1020

catcattatg ttgctaacaa ggatggctct gtgacagcat ttgttgattc tgtcactctt 1080

tacaagtacg agtatcgtaa tgttgctcaa aatgctgctg ttaaccaaaa tattgtattt 1140

agagttttaa caaaagatgg tcgtcctatt tttgaaaaag ctcataatgg taacaaaact 1200

tttgcagaaa ctttaaacaa aactttacaa ctcaatctta aatatgagct taaaccacat 1260

gcttccagcg gtaacgtcga agtctttaag attcatgatg actgggtaca tgacacacat 1320

gggtctgctt tagtgtctta tgttaataat aatgatgctg ttcctaatgt ggtcatccca 1380

gaacagccaa ctccaccaaa accagaaaaa gttacacctg aagcagaaaa accagtacct 1440

gaaaagccag ttgagcctaa attggtaacg cctgttttaa aaacttatac tccagtcaaa 1500

tttattccgc gagaatacaa accagtccca agtacccctg agacgtttac ccctgagaaa 1560

tttactccag ctcaaccaaa agtgaaacct catgtgtctg tacctgaaaa aattaactat 1620

aaagtagcgg tgcatccagt acagatacca aaggccacac caactaagaa agttctagat 1680

gaaaacgggc aatctattaa cggtaaatct gtattaccaa atgcaacatt agactatgtt 1740

gctaaacaaa actttagtca atacaaaggt attaaagctt ctgcagaagc tatcgcaaaa 1800

ggttttgcat ttgtagatca accaaatgaa gcgttagctg aattgactgt taagtctatc 1860

aaagcatcta atggtgatga tgtatcaagc ttgttagaaa tgcgtcatgt tttatcaaaa 1920

gatactttag accaaaaact tcaatctctt attaaagagg caggaattag tccagttggt 1980

gagttttaca tgtggactgc aaaagatcca caagcttttt ataaagctta tgttcaaaaa 2040

ggtctagata ttacttataa cttatcattt aaagttaaaa aagagtttac taaaggtcaa 2100

atcaaaaatg gtgttgcaca gattgatttt gggaatggat atacaggtaa tattgtagtc 2160

aatgatctga caactccaga agtccataaa gacgtgttag acaaagaaga cggcaagtct 2220

attaacaatg gtactgtcaa actcggtgac gaagtcactt acaagcttga aggatgggtt 2280

gtaccagcga accgtggtta cgatcttttt gaatacaaat ttgtagatca cttacaacac 2340

acacacgatc tttacctaaa agataaagta gtcgctaaag tagcaattac acttaaagat 2400

ggcactgtaa ttccaaaagg gacaaatcta gttcaatata ctgagactgt ctataataag 2460

gaaacaggtc gctatgagtt agcctttaag gcagacttcc tcgcacaagt ttcacgttct 2520

agtgcctttg gggcagatga ctttattgta gttaaacgta tcaaagcggg tgatgtttac 2580

aataccgcag acttctttgt caatggcaat aaggtaaaaa ctgaaactgt ggtaacacat 2640

actcctgaga aaccaaaacc agttatgccg caaaaagtaa ctcctaaagc accagcttta 2700

ccatccacag gagagcaagg ggtatctgtc ctaacagtac ttggtgccgc cttactctca 2760

ctcttaggcc ttgtagggtt taaaaagcgt caacagtaa 2799

SeqID 77

atgaatcaga taaaaattat cacaggactt acagtcgcaa cactatccgc agtggtaggg 60

aatgtatacg cagaagatat cacaccgaca gcaccagtta atgaaccaca agtatcaagc 120

gaaaccgcaa aaacgcctca agttacagaa agtcaagtta acagcgcaaa agttactgcc 180

gatcaagcaa caagtgatgt taatgcacaa aaaaatgtag ttaataatgc tcaaaatcaa 240

aaaaatcaag cacaacaaaa acttgttaac gcaactacaa cgttaaatga aacacaaaaa 300

ttagtgcaag aatctaccaa tcaaaatcaa gtacaacaaa cagttgattc cgcaaagcaa 360

aggttgtcgc agacagaagc taatcaaaaa attactcaaa ctgaacaagt caaagctcaa 420

aatcaagtta atgcacaaca aacagttgtt gttaacaatg agcatgatgt tgcaactaag 480

acagctgatg ttaaacaagc tcaagcatca gtcgatacag ctaaagatgc tttgactaat 540

actatagtta atagtgattt aaataaagca cagtcaaacg tcacaactaa gacagctgat 600

gttaaaactg cgacagatgc acttacaaaa gcacaagcga ctgataaaac acttactaat 660

caaaaagcaa aagcacaaca aatagttgat tcagcaaaac aaaacttatc tgctaaagat 720

acacagcttt cacaagctaa tgctgaggtc aatcatcaca agtttaaaac ggctctaggg 780

caaagtcatt attacaatca acgtgataat gcttgggctg gggtatatgg agggcataca 840

tttgcttcaa ctggatgtgt cccatcagca ttagcaatgg tttactctga tttatcaaat 900

cggacaataa cgccgagaga gatagctgat tacttataca acaacacaga tgaattcaat 960

aaacgtttcg gtggcacaag tgggaaaggt attatttctg ctactaaagc gtttggttat 1020

gttgtgactc atttggctag caaaaatgct ataactgaag cattaaaagc aggtcatcat 1080

gttgttgctg ctgtacaaaa taataaattc agtccctggg ggcctcaata cagtcacgaa 1140

attgtattga gaggtagttc taatggcaat acgtatgttt atgatccgta taaccgtgat 1200

aacaatggtt tttacagtgt tgaccgcatt tggaatgaac agtcacgaga tagtattgat 1260

actgctggtg taggtgtacc gtttttcgca attatgacta aaaatatggc taatgcttta 1320

actaagcagt cacaagcttt agcaagccaa caagttgctc aaaaacaatt aaatgatgca 1380

caagctaaag caacaggtct taatgcagta actatgcaga caccgattgc acaagctaat 1440

ttgattaaag cacagtcaaa tttaaaagat gctcaaaagc gattagcaga agcacaagca 1500

tcagtcaaat tagctaatca agataatgtt aaaaaacaag ctgacttaac aaaagcagag 1560

tctaaattaa aagatgctca aaagcaatta gcagcagcac aagctaaatt gacaacaagc 1620

aaaacaaaac ttaatcaatt aaaacaagtg ttagcagaag caagtcaaca agtagcccaa 1680

gcaaatcaag attacaagca agctaaagat aatctaacac aaaaaactgc ttatctaaca 1740

aatctacgca atgcacaagc taatttgatt aaagcacagt ctgatgtagc acaagctaaa 1800

gataacttag caaataagat tgctaagtta caaagagaag tagcttattt acaagagtta 1860

aaaactaaag cagtagatgc gcaatcacag tatcaaaaag ttttatcagc ttataagtca 1920

gttttatcag ctaaagcaag tttaaaatta gcggaagaga aagctcgact tgataaaaag 1980

ggtcacgaag cagtagcagt agttgacgaa acaggtaaaa ttactagcta tattacttct 2040

aaacacaaaa tagaaatgaa atctcttgtt gcaactaaga caactgatgt taaacaagta 2100

tcagttgcta aagcaagcgt gttgccaagt actggtgatg ttaaacaagt atcagttgct 2160

cttctgggta tgttattaac gttctctggt tttttaggta tacgtaaaca aagtaaaaaa 2220

gttattaatt aa 2232

SeqID 78

atgatatcac gtaaagttgc gcttgtgacg ggggcatctg ctggttttgg tgcagctatt 60

gttactaaat tagtttcaga tggttatagc gttattggtt gtgctcgtcg aatggataaa 120

ttaaaatgct ttggagaaaa gttttcagag ggttactttt atccacttca aatggatatt 180

acaagtagag aatcggttga caaagcttta gaaagtctgc ctaaaaattt gcaatcaatt 240

gatttgttgg ttaataatgc aggtttagca cttggattgg ataaatcata tgaagctgat 300

tttgaagatt ggatgactat gattaatact aacgttgtag gattaattta cttgacacga 360

tgcattttac cgaaaatggt agaagttaat agagggttaa ttatcaattt gggatcaact 420

gcaggaacaa ttccttatcc aggagctaat gtctatggcg catcaaaagc ttttgtaaaa 480

caattttctc tcaacttgcg tgccgattta gctggaacta aaatcagagt tactaaccta 540

gaacccggtt tatgtgaagg gacagaattt tcaactgtac gtttcaaagg agatcataaa 600

agagttgaaa aactttatga aggtgcgcat gcaattcaag cagaggatat tgctaatacc 660

gtgtcatggg ttgctagcca accagaacac attaatatta atcgcataga aataatgcca 720

gtcagtcaaa cttatggacc tcaacctgtt tatcgtgatt aa 762

SeqID 79

atgatttatt tagacaatgc tgctactacc gctctaaccc catctgttat tgagaaaatg 60

accaatgtca tgacaagtaa ctatggtaat ccatctagta tacatacctt tgggcgtcaa 120

gcaaatcaac ttttacgtga atgtcgacaa attattgctg aatatctaaa tgttaattca 180

cgtgaaatta ttttcacttc tgggggaact gagagcaaca atacagctat caaagggtat 240

gctcttgcaa atcagctaaa aggtaaacat attattacct ctgaaattga acatcattca 300

gtcctacata ctatgactta cttatcagag cgatttggtt ttgatattac ttacttaaaa 360

ccaaaccatg gacaaattac tgcaaaagac gttcaagaag ctttacgaga tgatactatt 420

atggtatctc tcatgtttgc taataatgaa accggggact ttttaccaat tcaagagatt 480

ggtcagcttc tcaggaacca ccaagctgtt tttcacgttg atgccgttca agtctttagc 540

aaaatggaac ttgatcctca ttctttagga attgactttt tagctgcttc tgcccataaa 600

tttcacggtc caaaaggtgt tgggatactt tactgtgctc cccatcactt tgatagtcta 660

cttcatggtg gagaccaaga ggaaaaaagg cgtgcttcaa ctgaaaatat aattggtatt 720

gctggaatgt ctcaagctct tactgatgct acgactaaca cccttaaaaa ttggactcac 780

attagtcagc tgagaacgac ctttttagat gctatttcag accttgactt ctatcttaat 840

aacggtcaag actgcttacc tcatgtactt aatataggtt ttcctagaca gaataatggc 900

ttgttattga cacagttaga tttagctgga ttcgcagttt caacaggttc tgcatgtact 960

gcaggaacag tcgaacctag tcatgtctta acaagcttgt atggagccaa ctcaccacgt 1020

ctaaatgaat caatacgtat tagtttttca gaactaaata cccaagaaga aattcttgaa 1080

ttagctaaaa ccttaagaaa aattatagga gattaa 1116

SeqID 80

atgtctagga aaacatttaa acatatacta tcgattggag tttgcacgct cgtactatcg 60

atgagtcttt attacactga aaaagcccac gctattgctg gtcctagtga ccgccaatac 120

gtagaaaacc caaatcctca cattattgta aatgttacag gtactgatca aaacggaaat 180

agcattttac cgcattacat cgaagtcaat gtaaagatgg gacaaacttt aagtaaagaa 240

gaaattctag attatattgc tcgaaattta aactctagtg ttggaggaga aagtaaaaac 300

gttcaataca gcaacatcga gtttaaggaa agtgcttatc tgaagcgtca attagatgat 360

ggcaagacag aagaaatagc aattgataac gacggtgtta ctgtacctaa agacggtcca 420

aacaaatttt ggattgacgt tccagtaact tgtactgtta ctcctatcgt aacagaaaca 480

catgaagttc gatgggggac tccagtcgct atatcacacc gtatttactt tgttgaagaa 540

tcttctggaa aagttttaga tgaatacaca aatctacaca ctgctgattc ggaacttaac 600

ggttatcgtg ttggagatta tatcacagac tatgcacttt ctaagtctgc ttacgaagct 660

tttttaaact ctcgtttaga taaagaaggt tacaaacttc aacatcgtat tagcacgaat 720

gtacgacaaa accttcaaat tgataaattg attttcaatt atgactttaa tgaggaaaat 780

atttactacc aaatcgggaa tatccgtcca ctattaagtc gctcatcagc tgaagtagaa 840

tctgacatca ttacagaacg ctactatgtt tctaaaaatg ctaaaagttt agcacgtaca 900

gaatcaacca tttcgattaa aatggttgat gccaaaactg aacaaccgct atttaaccac 960

acattaactg gttatcaatt ggcaactgtc tcccatgtct ataacagact ctttgaagaa 1020

aatcttatcc caactacaaa atcaggagaa agatatttta ttcaaaatat gaaaaaaaca 1080

gctgaacaag aatatactgt ttacctttca gaaacacctt attctaaaga gaacgctccc 1140

gtaatttctt atgatgcaag acctgttgat tgggattatc actcaggcgc ttcaggatca 1200

cttgaaaatc agcctaacat ctatactgaa gaagattcaa ctgaattttt gggtaataaa 1260

ccaaaagcag cttgttatcc aaacaaacaa tttgcttgcg aaaatactga ctctaaatac 1320

aactatagct atttagaaaa atag 1344

SeqID 81

atgaatcctt taataattgg aatgaatgat aaacaagcag aagcggtaca aacgacagac 60

ggaccgcttt tgattatggc aggagctggc tctggaaaaa cacgtgttct gactcatcgt 120

attgcttatt taatagatga aaaatatgtt aatccttgga atattttagc gattactttt 180

actaataaag cagcgcgtga aatgcgtgaa cgtgctatcg ccttgaatcc agctacccaa 240

gatactttga ttgcaacttt tcatagtatg tgtgttcgta ttttacgtcg tgaagctgat 300

tatattggat ataatcgtaa ctttacaatc gttgatccag gtgagcagcg tactttaatg 360

aagcgaatca ttaagcaatt aaatttggac acaaaaaagt ggaatgaacg ttcaatttta 420

ggtacaatct ctaatgctaa aaatgacctt cttgatgaaa ttgcatatga gaagcaagcc 480

ggagatatgt atacacaggt aattgctaaa tgctataaag cttatcaaga ggaattacgt 540

agaagtgagg ccatggattt cgatgacttg attatgatga cacttcgatt atttgatcag 600

aataaagatg ttttggccta ttaccaacag aggtatcaat atatccatgt agacgagtat 660

caagatacta accacgctca ataccaatta gttaagttat tagcttcgcg tttcaaaaat 720

atttgtgttg ttggtgatgc cgatcaatcc atttacggat ggcgtggagc tgatatgcaa 780

aatattcttg attttgaaaa ggactatccg caagccaaag ttgtattatt agaagaaaat 840

tatcgatcga ctaagaaaat acttcaagct gctaataatg tgattaatca taataaaaat 900

cgccgtccca aaaaattatg gactcaaaat gatgaaggtg agcaaattgt atatcataga 960

gctaacaatg agcaagaaga agccgttttt gtagcatcaa ctattgataa catcgttcga 1020

gaacaaggaa aaaatttcaa agattttgcc gttctttatc gtacgaatgc gcaatctcgt 1080

actattgagg aagcactttt aaaatccaat attccatata caatggttgg tggaacaaaa 1140

ttctatagtc gtaaagaaat tcgagatgtt attgcttatc tcaatatcct tgcaaatact 1200

tctgataata tttcttttga gcggattgta aatgaaccta aaagaggggt tgggccaggc 1260

actttagaaa aaatacggtc atttgcctat gaacagaaca tgtctcttct tgatgcctct 1320

tcaaatgtca tgatgtcgcc cttaaaaggg aaggctgctc aagctgtttg ggatttagct 1380

aatctgattt tgactctacg tagtaagcta gatagtttaa ctgtaacgga gattacagaa 1440

aacctactgg ataagacagg atatctagaa gcacttcaag ttcaaaatac attagaaagt 1500

caagcgcgta ttgaaaatat tgaagaattc ttgtcagtga ctaaaaattt tgacgataac 1560

cctgagatta cagtagaggg tgaaactggt ttagatcgtc tatcgcgttt tttaaatgat 1620

ttagctttaa tagcagatac tgatgatagc gccactgaaa cggctgaggt cactttaatg 1680

acattacatg ctgccaaggg tctagaattt ccagttgttt tcttaatcgg tatggaagaa 1740

ggagtatttc ctttatcgcg tgcaatagag gatgctgatg aattagaaga agagcgtcgt 1800

ttagcttatg tcgggattac gagagcagaa caaatacttt tccttaccaa tgctaatacc 1860

cgtactttat ttggtaaaac tagttataat agaccaaccc gttttatacg tgagattgat 1920

gatgagctga tacaacatca agggttggct cgaccagtta attcttcatt tggtgtgaaa 1980

tattcaaaag aacagcctac acaatttggt caaggaatga gtcttcaaca agcgcttcag 2040

gcacgtaaaa gtaattcaca acctcaagta actgcccaac tccaggctct taacgcgaac 2100

aatagccatg agacatcatg ggagattggt gatgttgcaa ctcataaaaa atggggggac 2160

ggtacggtgc ttgaagtttc aggtagcgga aagactcaag agttaaaaat aaattttcca 2220

ggtattgggt taaagaaatt attagcgagt gtcgctccta ttagtaaaaa ggaaaactaa 2280

SeqID 82

atgaaattat acgttcaatt aatggttatt ttaacctttt cattcgctgg tgaggttcta 60

tctactatat tcaacttacc tgtaccaggt agtattattg gattaatatt actattccta 120

gcgttaaaat acaaaatcat caggctcagg catattgatg ccgtggggaa ttttctatta 180

gcaaatatga ccattttatt tttaccacca gcagttggac tgatggagca ctttcaagac 240

ataaaaccct atctttttgg gatagctatc attatcttag gagcgctatt tctcaatatt 300

ctaactattg gactggtatc ccaatggatc aaaaagagat atgaaggaga ttatccagaa 360

attggaggta aaaatggcaa ctttaacgaa taa 393

SeqID 83

atgatttttg tcacagtggg gacacatgaa cagcagttca accgtcttat taaagaagtt 60

gatagattaa aagggacagg tgctattgat caagaagtgt tcattcaaac gggttactca 120

gactttgaac ctcagaattg tcagtggtca aaatttctct catatgatga tatgaactct 180

tacatgaaag aagctgagat tgttatcaca catggcggtc cagcgacgtt tatgtcagtt 240

atttctttag ggaaattacc agtcgttgtt cctaggagaa agcagtttgg tgaacatatc 300

aatgatcatc aaatacaatt ttttaaaaaa attgcccacc tgtatccctt ggcttggatt 360

gaagatgtag atggacttgc ggaagtgttg aaaaggaata tagctacaga aaaatatcag 420

ggaaataatg atatgttttg tcataaatta gaaaaaatta taggtgaaat atga 474

geqID 84

atggtgatga aaatcataga gttaaaagaa gcaaccgtac aagtcagtaa tggtttagca 60

gaaatgaaaa cgatattaga ccatgttaat ttgagtattt atgaacatga cttcattaca 120

atattgggtg gaaatggagc tggaaaatca acgcttttta atgtaattgc aggtaccttg 180

atgttaagcg gtggaaatat ttacatcatg ggacaagatg ttaataattt accagcagaa 240

aaacgagcta aatatttatc acgggtattt caagatccga aaatgggaac agcacctagg 300

atgacagttg ctgaaaattt attagttgct aaatttcgag gtgaaaagag accgttagtt 360

cctagaaaga ttactaacta taccgaagaa tttaaaaaat tgattgctag aactggtaat 420

ggacttgatc gccatttaga gacacctaca ggtttattat caggtggaca aagacaagca 480

cttagtttat taatggcaac tttgaaaaaa ccaaatctgt tattattgga cgaacacaca 540

gcagcgcttg atcctagaac aagtgtttcg cttatgggct tgactgatga gtttatcaaa 600

caagattcgt tgactgcctt aatgattaca catcatatgg aagatgctct caaatatgga 660

aatcgtatat tagtaatgaa agatggcaag attgtcagag atttaaatca ggcacaaaaa 720

aacaagatgg ctatagctga ctattatcaa ttatttgatt aa 762

SeqID 85

atggaaatca aaaagaaaca tcgtattatg ctttattcag cccttatttt aggaacaata 60

ttggttaaca atagttacca agctaaagct gaagagctta ccaaaactac ctcaacgtcc 120

caaataagag atactcaaac taataatatt gaagttctcc agactgaaag taccactgtc 180

aaagagacta gcaccacaac cacacaacaa gatctgtcta accccacagc ttcaaccgca 240

actgcaacag ccactcatag cacaatgaaa caagtagtag ataatcaaac tcaaaataag 300

gagctggtga aaaacggaga ttttaatcaa actaaccctg tatctggaag ctggtcacat 360

acaagcgcta gggaatggtc tgcttggatt gataaagaaa atactgctga taaatcacct 420

attatccaac gtaccgaaca aggccaagta agcctatcca gcgacaaagg ctttagaggt 480

gctgtaacac aaaaagtgaa cattgatccc actaaaaaat atgaggtcaa gtttgatatt 540

gaaacaagta acaaggctgg acaagctttc cttcgtatta tggagaaaaa agacaacaat 600

acgcgacttt ggctttctga gatgaccagc ggtactacta acaaacatac cttaacaaag 660

atatataacc caaagttaaa tgtctccgag gtgacacttg aactttatta tgaaaaagga 720

actggttctg ctacttttga taatatatca atgaaagcaa aaggccctaa agactcagag 780

catccacaac ccgtcacaac acaaattgaa gaaagcgtta atacggcttt aaacaaaaat 840

tacgttttta ataaagctga ctaccaatac actctaacca atccgtctct cgggaaaatt 900

gttggtggaa tattgtatcc aaacgctact ggttcaacaa ctgttaaaat atctgataaa 960

tctggtaaaa taattaaaga agtaccgtta tcagttacag cttcaacaga agataagttt 1020

acaaaactcc tcgacaaatg gaacgacgtg actattggta atcatgttta cgatactaat 1080

gattcgaaca tgcaaaagat taatcagaaa ttagatgaaa ctaacgccaa aaacatcaaa 1140

actatcaaac tggattctaa tcacactttc ctttggaaag atttagataa tctcaataat 1200

tcagcacagt taaccgctac ttatcgtcgt ttggaagatt tagctaaaca aatcaccaat 1260

ccccactcta ctatttacaa aaatgaaaaa gctattcgta ctgtaaaaga gagtctggct 1320

tggcttcatc aaaacttcta caatgttaat aaagatatag aaggctctgc caattggtgg 1380

gattttgaaa tcggtgtccc tcgctcaatt acagctaccc tagctctcat gaataactac 1440

ttcactgacg ctgaaataaa aacttatacc gacccaattg aacactttgt tcctgatgca 1500

ggatatttcc gtaaaacgct tgacaatcca tttaaagccc ttggtggtaa tctagtcgat 1560

atggggcgcg ttaaaatcat tgaaggttta cttcgtaaag acaatactat tatcgaaaaa 1620

acttctcatt ctctaaaaaa tctttttact actgctacta aagctgaagg tttctatgct 1680

gacggttctt acatcgacca tacaaatgtt gcttatactg gcgcctatgg taatgttctg 1740

atagatggtt tgacacaatt gctgcctatc attcaagaaa ctgactataa aatctctaat 1800

caagaacttg atatggttta taaatggatt aatcaatcat ttttaccttt aattgtaaaa 1860

ggtgagttaa tggatatgag tcgtggacgc tcaattagta gagaggcagc ttcttcgcat 1920

gcggctgcag ttgaagttct cagaggtttc ctcagattgg ctaacatgtc taatgaagag 1980

cgaaacttag acctcaaatc aactattaaa acgattatca cttcaaataa attctacaat 2040

gtcttcaata acctcaaatc gtattccgat attgccaaca tgaataagat gcttaatgac 2100

agtacagtcg ctactaaacc tttaaaaagt aatttatcaa cctttaatag catggaccgc 2160

ttagcttatt ataatgccga gaaagacttt ggtttcgcgc tttcattaca ttctaaacgt 2220

accctcaact atgaaggaat gaatgatgaa aatacacgtg attggtatac cggagatggt 2280

atgttctatc tttataatag tgatcaatct cattatagta atcatttttg gccaaccgtc 2340

aatccttata aaatggctgg aacaactgaa aaagatgcta agcgtgaaga taccactaag 2400

gaattcatga gcaaacatag caaagacgct aaagaaaaaa ccggtcaagt tacaggaaca 2460

tctgactttg ttggttccgt caaacttaat gatcactttg ctcttgccgc tatggatttt 2520

actaactggg atcgcacctt aacagcacaa aaaggttggg ttatcttaaa tgataagatt 2580

gtctttttag gtagcaacat caagaatact aacggcattg gaaatgtttc tacaacaatt 2640

gatcaacgaa aagacgattc taaaacacct tatactacat acgtcaatgg aaaaactatt 2700

gatttaaaac aagcaagttc tcaacaattt acagatacaa aaagtgtctt tttagaatca 2760

aaagaacctg gtcgcaatat tggttatatc ttctttaaaa atagcactat tgatattgaa 2820

cgcaaagagc aaacaggtac ttggaacagc attaatcgta cttctaaaaa tacctcaatc 2880

gttagcaatc cttttatcac tataagccaa aagcatgaca acaaaggtga tagctatggt 2940

cacatgatgg ttccaaacat tgatcgcaca agttttgata aattagccaa cagcaaagaa 3000

gtagaattac tagaaaatag ttcaaaacaa caagttatct atgataaaaa cagtcaaact 3060

tgggctgtta tcaaacacga taatcaagag agtctcatta acaatcaatt caaaatgaat 3120

aaagcgggac tttacctagt acaaaaagtt ggtaatgact atcaaaatgt ctattaccaa 3180

cctcaaacca tgacaaaaac agaccaatta gctatctaa 3219

SeqID 86

atgcattctt tttctaatcc tggatatcct tatgataatg ccgtaactga agcatttttc 60

aagtatttaa agcatagaca aatcaaccga aaacattatc aaaatatcaa acaggttcaa 120

ttagactgct ttgaatacat tgagaatttt tataacaatt acaacccaca tacggctaat 180

ctaggactaa cccctaatca gaaagaagaa aattatttta acgcaataaa ataa 234

SeqID 87

gtgaagaaaa catatggtta tatcggctca gttgctgcca ttttactagc tactcatatt 60

ggaagttacc aacttggtaa gcatcatatg ggtctagcaa caaaggacaa tcagattgcc 120

tatattgatg acagcaaagg taaggcaaaa gcccctaaaa caaacaaaac gatggatcaa 180

atcagtgctg aagaaggcat ctctgctgaa cagatcgtag tcaaaattac tgaccaaggc 240

tatgtgacct cacatggtga ccattatcat ttttacaatg ggaaagttcc ttatgatgcg 300

attattagtg aagagttgtt gatgacggat cctaattacc gttttaaaca atcagacgtt 360

atcaatgaaa tcttagacgg ttacgttatt aaagtcaatg gcaactatta tgtttacctc 420

aagccaggta gtaagcgcaa aaacattcga accaaacaac aaattgctga gcaagtagcc 480

aaaggaacta aagaagctaa agaaaaaggt ttagctcaag tggcccatct cagtaaagaa 540

gaagttgcgg cagtcaatga agcaaaaaga caaggacgct atactacaga cgatggctat 600

atttttagtc cgacagatat cattgatgat ttaggagatg cttatttagt acctcatggt 660

aatcactatc attatattcc taaaaaggat ttgtctccaa gtgagctagc tgctgcacaa 720

gcctactgga gtcaaaaaca aggtcgaggt gctagaccgt ctgattaccg cccgacacca 780

gccccaggtc gtaggaaagc cccaattcct gatgtgacgc ctaaccctgg acaaggtcat 840

cagccagata acggtggcta tcatccagcg cctcctaggc caaatgatgc gtcacaaaac 900

aaacaccaaa gagatgagtt taaaggaaaa acctttaagg aacttttaga tcaactacac 960

cgtcttgatt tgaaataccg tcatgtggaa gaagatgggt tgatttttga accgactcaa 1020

gtgatcaaat caaacgcttt tgggtatgtg gtgcctcatg gagatcatta tcatattatc 1080

ccaagaagtc agttatcacc tcttgaaatg gaattagcag atcgatactt agctggccaa 1140

actgaggaca atgactcagg ttcagagcac tcaaaaccat cagataaaga agtgacacat 1200

acctttcttg gtcatcgcat caaagcttac ggaaaaggct tagatggtaa accatatgat 1260

acgagtgatg cttatgtttt tagtaaagaa tccattcatt cagtggataa atcaggagtt 1320

acagctaaac acggagatca tttccactat ataggatttg gagaacttga acaatatgag 1380

ttggatgagg tcgctaactg ggtgaaagca aaaggtcaag ctgatgagct tgctgctgct 1440

ttggatcagg aacaaggcaa agaaaaacca ctctttgaca ctaaaaaagt gagtcgcaaa 1500

gtaacaaaag atggtaaagt gggctatatg atgccaaaag atggtaagga ctatttctat 1560

gctcgtgatc aacttgattt gactcagatt gcctttgccg aacaagaact aatgcttaaa 1620

gataagaagc attaccgtta tgacattgtt gacacaggta ttgagccacg acttgctgta 1680

gatgtgtcaa gtctgccgat gcatgctggt aatgctactt acgatactgg aagttcgttt 1740

gttatcccac atattgatca tatccatgtc gttccgtatt catggttgac gcgcgatcag 1800

attgcaacag tcaagtatgt gatgcaacac cccgaagttc gtccggatgt atggtctaag 1860

ccagggcatg aagagtcagg ttcggtcatt ccaaatgtta cgcctcttga taaacgtgct 1920

ggtatgccaa actggcaaat tatccattct gctgaagaag ttcaaaaagc cctagcagaa 1980

ggtcgttttg caacaccaga cggctatatt ttcgatccac gagatgtttt ggccaaagaa 2040

acttttgtat ggaaagatgg ctcctttagc atcccaagag cagatggcag ttcattgaga 2100

accattaata aatctgatct atcccaagct gagtggcaac aagctcaaga gttattggca 2160

aagaaaaata ctggtgatgc tactgatacg gataaaccca aagaaaagca acaggcagat 2220

aagagcaatg aaaaccaaca gccaagtgaa gccagtaaag aagaaaaaga atcagatgac 2280

tttatagaca gtttaccaga ctatggtcta gatagagcaa ccctagaaga tcatatcaat 2340

caattagcac aaaaagctaa tatcgatcct aagtatctca ttttccaacc agaaggtgtc 2400

caattttata ataaaaatgg tgaattggta acttatgata tcaagacact tcaacaaata 2460

aacccttaa 2469

SeqID 88

atgaaaaaag gtttttttct catggctatg gttgtgagtt tagtaatgat agcagggtgt 60

gataagtcag caaaccccaa acagcctacg caaggcatgt cagttgtaac cagcttttac 120

ccaatgtatg cgatgacaaa agaagtatct ggagacctca atgatgtgag gatgatccaa 180

tcaggtgcag gcattcattc ctttgaaccg tctgtaaatg atgtggcagc tatttatgac 240

gcggatttgt ttgtttacca ttcacatacc ttagaagctt gggcaaggga tctagaccct 300

aatttaaaaa aatcaaaggt tgatgtgttt gaagcgtcaa aacctttgac actagataga 360

gtcaaagggc tagaagatat ggaagtcaca caaggcattg accctgcgac actttatgac 420

ccacatacct ggacggatcc cgttttagct ggtgaggaag ctgttaatat cgctaaagag 480

ctaggacgtt tggatcctaa acacaaagac agttacacta aaaaggctaa ggctttcaaa 540

aaagaagcag agcaactaac tgaagaatac actcaaaaat ttaaaaaggt gcgctcaaaa 600

acattcgtga cgcaacacac ggcattttct tatctggcta aacgattcgg cttgaaacaa 660

cttggtatct cgggtattta tacagagcaa gagccctctc ctcgccaatt gaaagaaatt 720

caagactttg tcaaagaata caacgtcaag actatttttg cagaagacaa cgtcaatccc 780

aaaattgctc atgctattgc gaaatcaaca ggagctaaag taaagacatt aagtccactt 840

gaagctgctc caagcggaaa caagacatat ctagaaaatc ttagagcaaa tttggaagtg 900

ctctatcaac agttgaagta a 921

SeqID 89

ttgcgtaaaa aacagaaact accatttgat aaacttgcca ttgcgcttat gtctacgagc 60

atcttgctca atgcacaatc agacatcaaa gcaaatactg tgacagaaga cactcctgct 120

accgaacaaa ccgtagaaac tccacaacca acagcggttt ctgaggaagc accatcatca 180

aaggaaacta aaaccccaca aactcctagt gatgcaggag aaacagtagc agatgacgct 240

aatgatctag cccctcaagc tcctgctaaa actgctgata caccagcaac ctcaaaagcg 300

actattaggg atttgaacga cccttctcag gtcaaaaccc tgcaggaaaa agcaggcaag 360

ggagctggga ctgttgttgc agtgattgat gctggttttg ataaaaatca tgaagcgtgg 420

cgcttaacag acaaagccaa agcacgttac caatcaaaag aagatcttga aaaagctaaa 480

aaagagcacg gtattaccta tggcgagtgg gtcaatgata aggttgctta ctaccatgac 540

tatagtaaag atggtaaaac cgctgtcgat caagagcacg gcacacacgt gtcaggaatc 600

ttgtcaggaa atgctccatc tgaaacgaaa gaaccttacc gcctagaagg tgcgatgcct 660

gaggctcaat tgcttttgat gcgtgtcgaa attgtaaatg gactagcaga ctatgctcgt 720

aactacgctc aagctatcag agatgctatc aacttgggag ctaaggtgat taatatgagc 780

tttggtaatg ctgcactagc ttacgccaac cttccagacg aaaccaaaaa agcctttgac 840

tatgccaaat caaaaggtgt tagcattgtg acctcagctg gtaatgatag tagctttggg 900

ggcaagaccc gtctacctct agcagatcat cctgattatg gggtggttgg gacacctgca 960

gcggcagact caacattgac agttgcttct tacagcccag ataaacagct cactgaaact 1020

gctacggtca aaacagccga tcagcaagat aaagaaatgc ctgttctttc aacaaaccgt 1080

tttgagccaa acaaggctta cgactatgct tatgctaatc gagggacgaa agaggatgat 1140

tttaaggatg tcaaaggtaa gattgccctt attgaacgtg gcgatattga tttcaaagat 1200

aagattgcaa aagctaaaaa agctggtgct gtaggagtct tgatctatga caatcaggac 1260

aagggcttcc cgattgaatt gccaaatgtt gatcagatgc ctgcggcctt tatcagtcga 1320

aaagatggtc tcttattaaa agacaatccc caaaaaacca tcaccttcaa tgcgacacct 1380

aaggtattgc caacagcaag tggcaccaaa ctaagccgct tctcaagctg gggtctgaca 1440

gctgacggta atattaagcc agatattgca gcacccggcc aagatatttt gtcatcagtg 1500

gctaacaaca agtatgccaa actttctgga actagtatgt ctgcgccatt agtagcgggt 1560

atcatgggac tgttgcaaaa gcagtatgag acacagtatc ctgatatgac accatcagag 1620

cgtcttgatt tagctaaaaa agtattgatg agctcagcaa ctgccttata tgatgaagat 1680

gaaaaagctt atttttctcc tcgccaacaa ggagcaggag cagtcgatgc taaaaaagct 1740

tcagcagcaa cgatgtatgt gacagataag gataatacct caagcaaggt tcacctgaac 1800

aatgtttctg ataaatttga agtaacagta acagttcaca acaaatctga taaacctcaa 1860

gagttgtatt accaagcaac tgttcaaaca gataaagtag atggaaaaca ctttgccttg 1920

gctcctaaag cattgtatga gacatcatgg caaaaaatca caattccagc caatagcagc 1980

aaacaagtca ccgttccaat cgatgctagt cgatttagca aggacttgct tgcccaaatg 2040

aaaaatggct atttcttaga aggttttgtt cgtttcaaac aagatcctaa aaaagaagag 2100

cttatgagca ttccatatat tggtttccga ggtgattttg gcaatctgtc agccttagaa 2160

aaaccaatct atgatagcaa agacggtagc agctactatc atgaagcaaa tagtgatgcc 2220

aaagaccaat tagatggtga tggattacag ttttacgctc tgaaaaataa ctttacagca 2280

cttaccacag agtctaaccc gtggacgatt attaaagctg tcaaagaagg ggttgaaaac 2340

atagaggata tcgaatcttc agagatcaca gaaaccattt ttgcaggtac ttttgcaaaa 2400

caagacgatg atagccacta ctatatccac cgtcacgcta atggcaagcc atatgctgcg 2460

atctctccaa atggggacgg taacagagat tatgtccaat tccaaggtac tttcttgcgt 2520

aatgctaaaa accttgtggc tgaagtcttg gacaaagaag gaaatgttgt ttggacaagt 2580

gaggtaaccg agcaagttgt taaaaactac aacaatgact tggcaagcac acttggttca 2640

acccgttttg aaaaaacgcg ttgggacggt aaagataaag acggcaaagt tgttgctaac 2700

ggaacctaca cctatcgtgt ccgctacact ccgattagct caggtgcaaa agaacaacac 2760

actgattttg atgtgattgt agacaatacg acacctgaag tcgcaacatc ggcaacattc 2820

tcaacagaag atcgtcgttt gacacttgca tctaaaccaa aaaccagcca accggtttac 2880

cgtgagcgta ttgcttatac ttacatggat gaggatctgc caacaacaga gtatatttct 2940

ccaaatgaag atggtacctt tactcttcct gaagaggctg aaacaatgga aggcgctact 3000

gttccattga aaatgtcaga ctttacttat gttgttgaag atatggctgg taacatcact 3060

tatacaccag tgactaagct attggagggc cactctaata agccagaaca agacggttca 3120

gatcaagcac cagacaaaaa accagaagct aaaccagaac aagacggttc aggtcaaaca 3180

ccagataaaa aaacagaaac taaaccagaa aaagatagtt caggtcaaac accaggtaaa 3240

actcctcaaa aaggtcaacc ttctcgtact ctagagaaac gatcttctaa gcgtgcttta 3300

gctacaaaag catcaacaag agatcagtta ccaacgacta atgacaagga tacaaatcgt 3360

ttacatctcc ttaagttagt tatgaccact ttcttcttgg gattagtagc tcatatattt 3420

aaaacaaaac gccaaaaaga aactaaaaaa tag 3453

SeqID 90

tttagtgtaa cctattcaca gtctgaacgt acggttgttt tctcttttgg agaaataaca 60

tttagtagga gtcgctggac aaatggcttt gaaactagaa taccagtaga tgagtggtta 120

ggtcttgaaa aatataagag atattcaata gaattcttat atcatgttgc aaaattggct 180

acaatgatgc cttatcgtca agtttgcaaa gtaatagata gcactttgca aacaatcata 240

acaaaagact gtgttttaaa agcagtaaaa tttgtagaaa aattgttaaa agaaaaagaa 300

cgctatcgtt tttatttgga agagccaccc gaacgtaaaa aagtgaaaaa actgtatgtt 360

gagggtgatg gagtcatgat taaaagcaca gattctagag aggaaagaag gtatttagat 420

ttaacacatt ttgttattca tacaggctca aaaaaagttt ctactaaaag atatgaattg 480

caggacaagc acgaaatatt acagcttaat tatgataaag ctaaatataa tcttttagat 540

tatatttata ataactatga agtagatgac gatactattt taatcactaa ctctgatatg 600

ggtaaaggct atactagtag agtttttaag gaattaggaa aagcacttaa ggtaaagaaa 660

catgagcatt tttgggatat ctatcatgtt aaagaaaagt taagttcata ccttagaaaa 720

tatccaattg aattaaccga ttttgcttta gatgcggtaa aaaaatataa ttctgataag 780

cttgaattag tttttgatac tgttgaatca ctgatttgtg atgaacttga agatcaagaa 840

tttcagaagt ttaagaaaaa agtattaaat aatttcaaat atataaaacc agctcatctt 900

agaaatcttt caaatcgtgg tattggtatc atggaatcac aacacagaaa gataacgtat 960

agaatgaagc gacgtggcat gtattggtca aagtggggaa tctccacaat ggcaaatatg 1020

attatacttg aaagagctaa cggtttacga gaattatttt tcggttcttg gagaaaggta 1080

tacagtgagt ataaagaagg ttcatttagt gcagggcgac tttttaaaaa gacagatgaa 1140

ttagataaat tttctaagcc ccttctaaaa aatggcagaa aatggagtat aacaggaatc 1200

aaaacaaaat ag 1212

SeqID 91

aaagcaagca gcggtgatta tggcacaaaa agggaaataa tcactgctaa taaggataaa 60

tacagcattt caaagatgtg tcgctggctg aatatgccac gctcaagtta ttactatcaa 120

gccgtggagt cagtatctaa aacggagttt gaagaaacta ttaaaagaat ttttctcgat 180

agcgagtcta gatacggatc cagaaaaatc aaaatatgct tgaataacga aggtatcaca 240

ctttcacgtc gtcggattcg acgcattatg aagcgactca atttggtttc tgtttatcag 300

aaagccacct tcaaaccaca ttctagaggc aagaatgaag cccctattcc caaccactta 360

gacaggcaat ttaagcaaga aagaccacta caagccttag tcactgactt aacctatgtt 420

cgtgtaggca atcgttgggc ttatgtttgc ctcatcattg acctatacaa ccgtgaaatc 480

atcggcctgt ctcttggttg gcacaagacc gctgaactcg ttaagcaagc catacaaagc 540

atcccttacg ccctgaccaa agtcaagatg ttccattcag atcgtggcaa agagtttgat 600

aatcagttaa ttgatgaaat attggaagcc tttggaatca cacgttcgct tagtcaggct 660

ggttgtcctt atgacaatgc cgtagctgaa agtacgtatc gtgctttcaa aattgaattt 720

gtttatcaag aaacctttca atcgctggaa gaactagctc ttaagactga aaaggcaaca 780

cttttctgta caacatttat aaagtgttgc cttttcaggt ttttaccaat gctataa 837

SeqID 92

atgaagacaa gaaatcgtaa aggtggttat ttagcgaata ctgcaaatga gtacatcgac 60

tctaaacagg caattcattg cttgagtgta gaacttgaac cgcaaattag gtttgaagag 120

ggtcagccta ctggggagat tatcgcttat aaggcttggt tctctcaaaa agggcttccg 180

ccttttatgg tgaagtttga aaatgaagtg acactaccag catatatggt aatggtgcaa 240

tttgagaatc ttcaagcttg tgaggttggg ttcaatgttt atttcaaggc agacaatctc 300

aaggaggtca aataa 315

SeqID 93

ttgtccactt ttgattcagt tacaggaagg accaatcact tggaagctta tgaagggctg 60

aacctttctg aaaaatttgc cattttaagt cactttgaca aacttagaaa tgagctgcag 120

acaccatcta ttcagctagg ggagtttgat agggaaatgg aagctttttc aatcactttg 180

ggaaatgaac tgttgggtta tttagaggca aatggcagcc cctatgagtt gaagcgagaa 240

ttgaatcagg ccgaaatgat ggcagtcctt gagcttagcc gtcaactggt tgcaaaattt 300

tctacaaaac tagaggagct gggaattgat ttgggttcat ttcaaccaga ccaagtcaac 360

atcttattgg atgccgttgg tcgttttcgc ttgaaaaatg cggacattgc tttattaggc 420

ggttatccaa aagcgagtgt ttcccagcta gctcttgcga cagaactcct ccagatggga 480

ctaagtcatg ataaggtaga atttttctta accagtcagc ttcagttaga ggatatgcga 540

caggtcgctt ttgctttcct acatgaaagc ttgaccagag aagaagcaga gcaatttgaa 600

acagaccgct ttcgtcatac aagcttaaac tttcgagaat ggcgagaact tctagaaaag 660

caagagccag aaatggtgga gatgtcagac gtcagcccgc ttgttcggga agtcttgcat 720

cattatccac ttggttcaag ggtcacttat aaaggacagg agtttgagat cttgtctatt 780

gaagctgcgg acatggataa tctgattcga atagagcttc aaaatgattt ttcatatctc 840

attgaacaaa atcccgttct ctatttccaa aacttggcag aaatcaggca agttcttcat 900

ttgtctcgtt cggaaattgt agaaagagag gaacaacctg aggaagaatg gactctcttt 960

tcgtttatgg atgaggggac agaggataac gaaaaggaac ctgagattgt ttttgaatct 1020

accgataaag ttgttacatt agatagtcag ccagcacaag taagtgagac tatatccgaa 1080

tcagttcctg aaaccaaaga ggtgattgaa gcagaacaag caatagcagt tgattttagc 1140

tttcctgaag atttgactaa tttttacccc aaaacagcta gggataaagt agtggcgaat 1200

ctcgtagcta ttcgtcttgt aaaagaatta gaaagtgcta ataaatcagc aacacctaat 1260

gaacaagaaa ttcttgccaa atatgtgggt tggggtggtc ttgccaatga attctttgat 1320

gagtacaatc cgaagttttc taaggaaaga gaggagttga aaactctcgt ttctgataaa 1380

gagtattccg atatgaaaca atcatctcta acggcctact atacggatcc gcttttaatt 1440

cgtcagatgt ggtctaagtt agaacaagac ggctttagtg gtggaaaaat attagaccca 1500

tcaatgggaa cagggaattt ctttgcggct atgccagctc atttgaggga aaagagtgag 1560

ctgtgtgggg ttgagttaga tactatcaca ggagcgatag ctaagcagct tcattcaaat 1620

gtccatattg aagtaaaggg atttgagacg gttgctttta atgataatag ttttgatttg 1680

gttatttcaa atgttccctt tgccaatatc cgtatcgcgg ataatcagta cgataagccc 1740

tatatgattc atgactactt tgtcaagaaa tctcttgatt tggtacatga tggtgggcaa 1800

gtagccatta tttcctccac aggaaccatg gacaaacgga cagaaaatat tctccaagat 1860

attcgtgaga cgacggattt tcttggtggc gtgcgtttgc cagatacggc ttttaaggcc 1920

attgcaggta caagtgtgac aacggatatg cttttctttc agaaacatct gaacaaaggc 1980

tatcaagcag atgacattgc cttttcaggt tctgttcgtt atgacaagga cgagcgtatt 2040

tggctcaatc cctactttga tggggagtat aatgcccagg ttcttggaag gtatgagatt 2100

aaaaacttta acggtggaac actttcggtt aaggaaacga ctgataacct gattgcgagt 2160

gttcgagaag ccttgcaaca tgtaaaagca ccaagggtta ttgataaaac agaagtcatg 2220

attaactcgg atgtgattgc aagacaggtc atagatacca ccattccacc tgaaattaga 2280

gaaaaccttg aacaatatag ctatggttac aaaggttcta ccatttatta tcgtgataat 2340

aaggggattc gtgttgggac aaaaacggag gaaattagct attatgtgga tgatgaggga 2400

aactttcagg cttgggagtc caagcattca caaaaacaga ttgaccgttt taataactta 2460

gaagtcacag atagcacagc tcttgatgtt tatgtgactg aagaacctgc gaaacgtggg 2520

caatttaaag gatatttcaa aaaggcggtt ttttatgaag ctcctttgtc tgaaaaagaa 2580

gtggcacgga ttaaaggaat ggtagatatt cgcaatgcct atcaagaggt cattgctatt 2640

caacgcaatt atgattacga taagaatgag tttaaccgct tgttaggaaa cctcaatcgc 2700

acctatgata gctttgtcaa acgctatggc tttttgaaca gtccagttaa ccgaaattta 2760

tttgatagtg atgataagta ttcgctttta gctagtctcg aagatgaaag tcttgaccca 2820

agtgggaaga cggtcattta tacgaagtca ctggcttttg aaaaagcctt ggtccgtcct 2880

gaaaaaatgg ttaaagaggt gtcaaccgct cttgatgcac tcaactccag tcttgcggat 2940

ggtcgtggag ttgactttga ttacatggct tccatctatc aaacagcatc taaggcagct 3000

ttgattgagg agctgggaga tcagattata ccagatccag aaagttattt aaaaggacag 3060

ctgacctatg tatctcgcca ggagttttta tcaggagata ttgtgacaaa attagaagtt 3120

atggacttac tgttaaagca agacaatcat gattttaact gggcgcatta tggtaatttg 3180

ttagaaagtg ttcgtccagc acgagtgatg ttggcagata ttgattatcg aattggttca 3240

cgttggattc ctttagctgt ttatggaaaa tttgtccaag aagcctttat ggggaagaat 3300

tatgacttaa cggctacaga agtggaagag gtcctctctg tcagcccgat tgatgggacg 3360

atggaatttc ggacacggtt tgcctatacc tactcaacag cgacggatag aagcctgggt 3420

gtagcaggct cacgctatga tagtggtcgg aaaatctttg agaatctgct taactccaat 3480

cagccaacca tcacgaagca gattcaagag ggagataaga aaaagaatgt gacagatgtg 3540

gagaagacaa ccgttctgcg tgctaaagaa gcacaaatcc aagacctctt tcaagatttt 3600

gtcgcaagct atcctgaagc ccaacagatg attgaggata cctataatag tctctataat 3660

cgtaccgtct ctaaagttta tgatggcagc cgtttggaga ttgatggttt ggctcaaaat 3720

atttcgcttc gtcctcatca aaaaaatgcc attcagcgaa tcgtggagga aaaacgggct 3780

cttttagctc atgaagtggg ttcaggcaaa actttaacca ttcttggagc aggctttaaa 3840

ctgaaagagt taggtatggt acataaaccg ctttatgtgg taccttctag cttgaccgct 3900

cagtttggtc aagaaatcat gaagttcttc ccaacgaaga aagtctacgt gacgacaaag 3960

aaagattttg ccaaagctcg gcgcaagcag tttgtctcac gtattattac aggggattac 4020

gatgccattg tcatcgggga ctcccagttt gaaaaaatcc ccatgagtca ggaaaaacag 4080

gtgacttata ttcaagataa gttggaacaa ttacgggaga taaagcaagg aagcgatagt 4140

gactacacag tcaaagaagc ggagcgttcg attaaaggtt tggaaaacca attggaagaa 4200

ctccagaaac tagatcgaga tacctttatt gaatttgaaa atcttggcat tgattttctc 4260

tttgtggacg aagcccatca tttcaaaaat attaggccga ttactggtct gggaaatgta 4320

gcgggaatta ccaacacgac ttctaaaaag aacgtggata tggaaatgaa agtgagacag 4380

attcaggcag agtatggaga tagaaatgtc gtttttgcga caggaacgcc ggtgtctaat 4440

tccattagtg aactctatac tatgatgaac tatattcagc cagatgtcct agaacgctac 4500

caagtttcta actttgactc ctgggttgga gcttttggca atatcgaaaa ttcgatggag 4560

ctagcgccaa caggagataa gtatcaaccc aagaaacggt ttaaaaagtt tgtgaatcta 4620

cctgaactca tgcggattta taaagaaacc gctgatattc aaacgtcaga tatgttggat 4680

ttacctgtac ctgaagccac agtcattgcg gtggaaagtg aactcacaga agctcagaaa 4740

aactaccttg aagaattggt ggaccgttca gatgcgatta aatcaggaag cgttgaccct 4800

agtgtcgata acatgttaaa ggtgataggc gaagccagaa agctagccat tgatatgcgt 4860

ttgattgacc ctgcctatac cttatctgac aatcagaaga ttatgcaagt agtggataac 4920

gtggaacgga tttaccgtga gggcaaagga gataaggcta cacagatgat tttctctgac 4980

attggcacgc ctaaaagtaa agaagaagga tttgatgtct ataatgaatt gaaagccctc 5040

ttagttgacc gaggaattcc aaaagaagaa attgcctttg tccatgacgc taatacggat 5100

gagaagaaaa attctttgtc acgaaaggtc aatagtgggg aagtaaggat tctcatggct 5160

tcaactgaaa aaggaggaac aggattaaac gtgcaagcac gcatgaaagc tgttcaccat 5220

ctagacgttc cttggcgtcc ctcagacatt cagcaacgca atggacgttt gattagacaa 5280

ggaaatcaac atcagaatgt agagatttat cattatatta ccaaaggttc atttgataac 5340

tatttgtggg ctacacaaga gaataagttg cgttatatta aacaaattat gacttcaaaa 5400

gatcctgttc ggtcagcaga agatattgac gagcaaacca tgaccgcttc agatttcaaa 5460

gcgttagcta ctggcaatcc ctatctcaaa ctcaaaatgg agttagagaa tgaattgacc 5520

gtcttagaaa atcagaaacg tgctttcaat cgctccaaag atgaatacag gcacactatt 5580

tcctattgcg agaaaaactt acctgttatg gaaaaacgat taaggcagta tgacagagat 5640

attgagaaat cacaagcaac gaagaatcaa gagtttatca tgcggtttga taaccagaca 5700

attgacaatc gcagcgaagc tggggattat ttacgaaaac tcatcaccta taatcgttcc 5760

gaaaccaaag aagtcagaac cttagcgacc tttagaggct ttgagcttaa aatggctaca 5820

cgaagtcctg gtgaaccctt gtctgacatg gtgtctctaa ctatttcagg ggataaccag 5880

tattcagttt cccttgattt aaaatcagac gtgggaacga ttcaacggat taacaacgcc 5940

attgaccata tcctagagga taaggaaaag acagaagaga tgacaaacaa cctcaaagat 6000

aagctagcag tcgcaagagt tgaagttgag aaagtctttg caaaggaaga ggaatatcag 6060

ttggtaaagg ccaagtatga tgtacttgcc ccattagtgg aaagagaagc agacttagaa 6120

gaaattgacg tagcactttc ccaatttagt agctctgacc catgcttgaa aaaagatcaa 6180

cagttagtcc ttgacattta a 6201

SeqID 94

atgaccacaa aatgcaatca tcattttctc atcaatcagg agaaaggcga aaagtatgtt 60

tttcgtaaaa gtaagcaata tcgcacgctg tgttcggttg ctttaggaac agttgtgatg 120

gcattcgtag ctttggccgg accgatggtg caggcggatg aagtagggag aactgttgcg 180

acaagtgtac agacggaaac gaatcctgca acaaatttaa aagaaaatca gccaagtccg 240

attgcggaac aaaaagatag tcttgcagca accggtcaat caactggaac ggtgacagtc 300

actgttccac atgataaggt gacacaagct gtcgataagg caaagaccga aggaataaaa 360

gcagtacaag acaagccgat ggacttaggc aatacagtat ccgcagctga aaccagccaa 420

caactcaaaa aggcagaaga agatgccaca aaccaaacaa caactatttc taaaactgtt 480

gaaatctaca agtctgataa agcaacttat gaagctgaaa agaagtgggt agagaagcgt 540

aatgaagagt taactgctgc ttatgataag gcagaacaaa cagggactgg cttaaaccat 600

tcggttgata cgactgtttc agaattgaag tcgcaagacc aaaacgctca tgtgaccgtg 660

aacacacaaa cagtaaaatc aggagatggg acgagtgttt caggctatca ggagtacgtc 720

aagtctgttg cggccattga taagaagaat aaagcgaact tagctgatta tcggactaag 780

aaacaagccg cagacgctgt tgtggctaag aatcaactca ttcaaaaaga gaatgaagct 840

gggcttgcta aggcaaaagc agaaaatgaa gcgattgaca gacggaataa agaagggcaa 900

aaagctgttg atgaagcaaa taaggctggg caagccgcag tagagcaagc gaaccaagaa 960

aaacaaaaac aggccgcaaa ccgtgctttt gaaattgcga caattaccaa acggaataaa 1020

gaaagagaag aagtcgcaaa gaaagaaaat gcagcgattg atgcttataa tgcgaaagaa 1080

tggatccgct ataagcggga tttagcaaac atctcaaaag gggaggaagg ctacatttca 1140

gaagcccttg cgcaggctct agatttaaat catggcgaac cgcaggtcaa acatggtgca 1200

ggtactcgaa atccagatcg aatcatttca aagggagatg ccatgttggg tggctattct 1260

aacattcttg attcaacggg tttctttgtc tacaatcact ttaaaacagg tgaaacgctg 1320

aactttacct atcaaaatct gaagcatgca cgttttgatg ggaagaaaat tacagccata 1380

acttatgata ttaccaatct ggtttcacca actggaacca atgcggtgca gttagttgtt 1440

ccaaacgacc caacagaagg ctttattgct tatcgcaatg atggcgctgg aaattggcgg 1500

acagataaga tggagttccg tgtcaaagct cggtatttct tagaagacgg ttcacaagtg 1560

acctttacta aagaaaaacc aggtgtcttt acccactcgt cactcaatca taatgatatt 1620

gggcttgaat atgttaaaga ctcatcaggt aaatttgttc ctattcatgg ttcaagtgtg 1680

caagtaacca atgagggctt agcccgttcg ttaggttcaa accgagcgag tgacttgaag 1740

ttgccagaag aatgggatac gacttctagt cgctatgctt ataaaggagc gattgtatca 1800

acagtcacat cggggaatat ttataccgta acctttggcc aaggagatat gccaacccaa 1860

gtgggaggga agacctattg gtttgcttta aatactttgc cagtcgcaaa aacagtgact 1920

ccctacaatc caaagactca tgtaagacca cagctggatc cagttcctga accgataaaa 1980

gttacaccag aaacttatac tcctaaaatc tttactcctg aaaaaccagt aacctttact 2040

ccaaaatcag tagaaaaagt gccccaacct agtttgacct taacaaaagt cacactacca 2100

acaaatctga agctagaacc attacccaaa gctccacaaa agccaaccgt tcattaccac 2160

gattacctct taaccacaac acccgctatc gcaaaagaag tgatgaatgt tgacaaagtt 2220

aatcttcatg gtaaacaggt ggctaaggat tccactgtta tttatccctt gacagtagat 2280

gttttatctc caaatcgttc caagataacc agtcttatct ttgaagatta tctgcctgct 2340

ggttatgcgt ttgatatgac gaagacacaa gcggagaata gcgactatga cttaaccttt 2400

gataaaaata agaactttgt gaccttgaaa gcgaaagata gcttacttca aacgttgaat 2460

aaagagttaa acaagtctta tcaactgtct gctccaaaac tttatggttc agttcaaaat 2520

gatggggcga cttattctaa tagctataaa ctccttatta acaaggacac cccaaacacc 2580

tatacggtta tttcaaacgt agttagaatt cggactccag gagacggtga aacgaccagc 2640

cgaatccggc ctaaaaagga caatgaaaat gcggacggtg tgttgataaa tgatacggtt 2700

gttgctctta gtacaaccaa tcattaccgt ttgacctggg atttggatca gtacaagggg 2760

gatacttctt caaaagatac cattgcacga ggattccttt ttgtagatga ctacccagaa 2820

gaagcacttg acttggtaga taaagggaca gttatcacca cccttgatgg taaagctgtt 2880

tcaggtattt ctgtatacag ctatacatcc cttgataaag caccaaaaga gctacaagat 2940

aaattagctc gtgcgaacat ttcgccaaaa ggagcgttcc aggtctttga accggacaat 3000

catcaagcgt tttacgatac ttacgttaag actgggcagt ctttagccct tctcacaaaa 3060

atgaaggtaa aagatagttt gtacggtcag actgtcagat acaaaaataa agcctaccaa 3120

gttgattttg gcaatggcta tgaaactaag gaagtcgtca atacagtcgt tcatccagag 3180

cctaagaaac aaaatctcaa taaggacaag gtggacatca atggcaaagc gatgcttgtc 3240

ggctcccaaa acttctatac gctttcttgg gatttagatc agtatcgtgg cctacaagcg 3300

gataagagtc agattgctca aggcttttac tttgtggacg attatcccga agatgttctt 3360

cttcctgaca caaaagccat tcaaattatg acgaaagacg ggaaagctgt taaaggaatg 3420

gagataaaaa cctatcatca actctcagat gctccaaagg aattgcaggc agctcttgcc 3480

aaacgtaata tcacgcccaa aggcgctttt caagtcttta tgccaaaaga tccacaagct 3540

ttttataaag cttatgtgac aactggtcaa aacttgacta ttgtgaatcc gatgacggtt 3600

cgtgaagcag tatacaactc agggaagtct tatgataatg tggcctatca agtggacttt 3660

ggacaggcct atgaaacgaa tatagtgacc aatcacgtgc caacagtaaa cccgcataag 3720

tccaatacca ataaagaagg tgtgtcaata gaaggtaaga cagtccttcc gaatacggtc 3780

aactactata aaattgttct agattatagt cagtacaaga atatgattgt gacagatgat 3840

gtcctcgtca aaggttttta tatggtggat gattacccag aagaagcact tactcctcat 3900

ccagatggga ctcaagtgat ggatcagaac gggaaatttg tcagaggcct gtctgttcga 3960

acttacgcaa gtttggcaga tgcaccgaaa gcagtacaag aagcaatgaa atctcgaaat 4020

ttcattccta aaggagctat tcaggtcttt caggcagatg acccaaaaac tttctttgaa 4080

acttatgtga aaacaggaca aaaattggtt gtgacaacac cgatgacagt gaagaatgaa 4140

cggattcaaa caggcggtca gtatgagaat acagcttatc agattgattt tggcatcgct 4200

tatgtaacgg aaacagttgt gaataatgta ccaaaattag aacctcaaaa agatgtggtc 4260

attgacttat ctcaaaaaga taagagttta aacggaaaag caatcgcttt ggatcaggtc 4320

tttaactacc gcttggtagg ttcacttatc ccacgcaatc gtgcgacagc cttggtggaa 4380

tacagtttta aagatgacta cgatgaaaag cacgatgaat ataagggtgt ttataaagcc 4440

tatactttgc gtgatgtaac cttaaaagat ggcacagtat taaagcaggg gacagaagtg 4500

acgaaataca ccttacaaag tgttgataag gcaaaaggaa cgattgcgat tcgttttgat 4560

acagcttttt tggaaaatat agctgatgaa tcagagtttc aggctgagct ttatcttcag 4620

atgaaacgca ttgcttcagg tgatgttgaa aatacagtta ttcatagtgt caatggttat 4680

aatattcgct ctaacacggt taagaccaca accccacaac cagagtcacc gactcctgat 4740

aacccaccgt caccccaacc gccagttcca accacagaat ccccagttca agctagtgtc 4800

ctaccaagta caggggaaag tcaatccctt ttggcgctca ttggaggagg tctcctgctt 4860

ggcctcgctt acggactttc taaacgaaaa aaggagaaaa actaa 4905

SeqID 95

atggcaaata ataattttta taatcgtgac ccttttggaa atatggatga tatttttaat 60

tctctaatgg gaaatatggg gggctataat agtgagaata aacgatactt aatcaatgga 120

cgggaagtta ctcctgaaga attcagtcaa tatcgtcaaa ctggaaaact ccccggtcaa 180

gagctaaata accaaaatac tcctacaaac caagtctctg cagatagtgt tttgactaaa 240

ttaggaacta atttaaccga tcaagcacgt cagcatttgc tagatccagt tattggacga 300

aacaaagaaa ttcaggaaac tgcagaaatt ctagctcgtc gtactaaaaa taatcctgtt 360

ctagttggtg atgcaggtgt tggtaaaaca gctgttattg aaggtttggc acaagcaatt 420

ataaatggtg atgtccctgc tgctatcaaa aataaagaaa tcatttcgat agatatctct 480

agccttgaag ctggcaccca atataggggt agttttgaag aaaatattca aaatatcata 540

aaagaagtta aggaaacagg aaatattatt ctcttctttg atgaaataca ccaaatatta 600

ggagccggtt caactggtgg tgatagtggc tctaaggggc tcgcagatat acttaagcct 660

gcactttcac gtggtgaatt aacggttata ggtgcaacta ctcaagatga atatcgtaac 720

accattctca agaatgctgc cttagcacgc cgctttaacg aagttaaagt caatgcacct 780

tctgcacaag atactttcaa tatccttatg gggataagaa acctctatga gcaacatcat 840

aatgttgttt tacctgactc tgttttgaaa gcagctgtag atttatctat tcaatacata 900

ccacaaagaa gtctccctga taaagcaatt gaccttattg atatgactgc tgcacaccta 960

gctgcgcaac atcccgtaac tgaccttaaa tccttagaaa aagaaatcgc tgcgcaaaga 1020

gacaagcaag aaaaagctgt caatactgaa gattttgaag aagctcttaa ggtcaagaca 1080

cgcattgaag agttacaaaa ccagattgat aatcataccg aaggacaaaa agtcactgca 1140

actatcaatg atatcgctat gtcaattgag cgtctaactg gagttcctgt atcaaatatg 1200

ggtgctagtg atattgaacg cttaaaagaa cttggcaatc gtctaaaagg taaagtcatt 1260

ggtcaaaatg atgctgttga ggcagttgca agagctattc gccgaaatcg tgctggtttc 1320

gatgatggta atcgtccgat tggtagcttt ctatttgttg gaccaactgg agttggtaaa 1380

actgagctag caaagcaatt agcttttgat atgttcggat caaaggatgc tatcgttaga 1440

cttgatatgt ctgaatataa tgatcgtact gcagtttcta agctaattgg agcgactgct 1500

ggttacgttg gttatgacga caatagtaat acactaacag aacgtatccg tcgaaatcct 1560

tattctattg ttcttcttga tgaaatcgaa aaagctgacc cgcaagttat cacgcttctc 1620

cttcaagttc tagatgatgg tcgtttaact gatggtcaag gaaacactat taactttaaa 1680

aatactgtta tcattgcaac ctcaaatgca ggttttggaa acgaagcatt tacaggtgac 1740

agcgataaag acttgaaaat tatggaacga atttctccat atttccgtcc agaatttcta 1800

aatcgtttca atggtgttat tgaattctct cacctaagca aagatgactt aaacgaaatt 1860

gtagatttga tgcttgatga agttaaccaa acaattggca aaaaaggaat tgaccttgtg 1920

gtagatgaaa atgttaaatc acacttaatt gacctgggtt atgacgaagc aatgggagta 1980

cgtccattgc gccgtgtcat cgagcaagaa attcgagatc gcatcacaga ctactatctc 2040

gatcatacag atgttaaaca cctaaaagct aatttgcaag atggccaaat cgtcatttct 2100

gaaagataa 2109

SeqID 96

atgggaagat ttaaagaact tttagagagt aaaaaagctc tcatcctaca cggtgccttg 60

gggaccgagc ttgagagtag aggttgtgat gtttctggta aactttggtc agacaaatat 120

ctgattgagg atccagcagc tattcagacc attcatgaag attatattcg tgctggcgct 180

gatattgtga cgacatcaac ttatcaggcg actttacaag ggctagctca agttggtgtc 240

tctgaaagtc aggcagaaga cttgattcgc ttgactgttc agttggcaaa agctgtgcgt 300

gagcaagttt ggaaaagttt gacaaaagaa gaaaaatcag aaagaatcta tcctttgatt 360

tctggggatg ttggtcccta cgctgccttt ttggcggacg gttctgagta cacagggctc 420

tacgatattt ataaggaagg tctcaaaaat ttccatcgcc atcgtattga gcttctttta 480

gatgagggtg ttgaccttct ggcacttgaa accattccaa atgctcagga ggctgaggct 540

cttattgagc tacttgtcga ggatttccct caggttgagg cttatatgtc attcacctct 600

caagatggta agaccatctc agatggtagt gctgttgcag gcttggcgaa agctattgat 660

gttagcccac aggtcgtggc gctgggcatt aactgttcaa gtccatcgtt agttgctgat 720

ttcttgcaag caatagcaga gcagacggac aagcctcttg tgacctatcc aaactctgga 780

gagatttatg atggggcaag ccaatcttgg caatctagcc gagatcattc acacacccta 840

cttgaaaata caagtgactg gcaaaaactt ggcgcccaag ttgtcggagg ttgttgccga 900

actagaccag ctgatattgc tgacctctca gaacatttga cataa 945

SeqID 97

atgaaaatag gaattgataa gattggtttt gcaaccagtc aatatgtcct tgaaatgact 60

gatttggcta ttgctcgcca agttgaccct gaaaaattca gcaagggatt attgttagat 120

tcacttagta ttacgccagt tacagaagat atcgttacac ttgcagcctc tgcagctaat 180

gatattttat ctgatgagga caaagaaact attgatatgg tgattgtcgc tacagaatca 240

agtatcgatc agagtaaggc ggcttctgtc tatgtacatc aacttttaga gattcaacct 300

ttcgcaaggt cattcgagat gaaagaagca tgctatagcg caactgctgc tcttgattac 360

gctaaattac atgtagaaaa acatccagat tctaaagtat tagtgatagc ttcagatatt 420

gctaaatatg gcattaaatc tactggagaa tccactcaag gtgcaggcag tatcgctatg 480

cttattagtc aaaacccatc cattctagag ctaaaagagg accgtctagc gcaaacccgt 540

gatattatgg acttttggcg accaaattat tctgatgttc cttatgttaa tggcatgttt 600

tcaacaaaac aatatctaga tatgctaaaa actacttgga aagaatatca aaaacgtttt 660

aatactagtc taagtgacta tgctgctttt tgtttccata tcccttttcc aaaattagct 720

ttaaagggct ttaataaaat tctagataat aacctagatg aacaaaagaa agctgaacta 780

caagaaaatt ttgagcactc tattacttat agtaagaaaa ttggaaattg ctatactggc 840

tcattgtatc taggacttct atcgttacta gaaaatagtc aaaatttaaa agcaggtgat 900

caaattgcct tcttttcata cggtagcgga gctgttgctg aaatttttac cggtcagtta 960

gtagacggct accaaaataa acttcagagc gatcgtatgg atcaactcaa taaacgtcaa 1020

aaaattacag taactgagta tgagaaactc ttctttgaaa aaacaatcct agacgaaaat 1080

ggaaatgcca acttcaacac ctaccgtact ggcacttttt ctctagactc tatttgcgaa 1140

catcaacgta tctacaagaa aattaataat taa 1173

SeqID 98

atgaaaagtg cttatatctt ttttaatcca aaatctggta aagatgaaca agcattggca 60

caagaagtaa aatcttattt aatagaacat gattttcaag acgattatgt tcgtatcatt 120

acaccttcat ctgttgagga agctgtcgcc ttagcaaaaa aagcatcaga agaccatatc 180

gacttggtta ttcctttagg gggagatggc actattaata aaatctgtgg tggtgtctac 240

gctggtggtg cttatcctac tatcggacta gtacctgccg ggaccgtcaa taacttttca 300

aaagctttga acatcccaca agagaggaat ctcgctcttg aaaaccttct aaacggtcat 360

gtaaagtccg tagatatctg taaagttaac gacgattaca tgattagcag tttaacactt 420

gggctccttg ctgatattgc tgctaatgtc acttctgaaa tgaagcgtaa attaggacct 480

tttgctttcg taggtgatgc ctaccgtata ttaaagcgta atcgatcata ttctattact 540

cttgcttacg ataataatgt acggtcttta cgcacgcggt tactacttat taccatgacc 600

aattcgattg ccggtatgcc agccttctct ccagaagcaa caattgatga tggacttttt 660

agagtctaca ctatggaaca tattcacttc ttcaaattat tgttgcatct aagacaattt 720

cgtaagggag attttagtca agcaaaggag ataaaacatt ttcataccaa taatctgact 780

atttcaactt ttaaacgtaa aaaatcagct atccctaaag ttcgtattga tggcgatcca 840

ggtgaccaat tgcctgtaaa agttgaagtc atccctaaag ccttaaagtt tatcattcct 900

aactcattac cataa 915

SeqID 99

atgacaacat ttactgctaa atttattgac caagagtggg aagtcccagt agaatcaggt 60

cgctaccata tgattgttgg ggaattttgt ccttatgcac aacgtccgca gattgcacgc 120

caactgcttg gtttggataa acatatttct atcagttttg tggatgatgt tccaagtgat 180

ataggactta tttttagcca accagaacag gttactggtg ctaagtcgct tcgtgatatt 240

tatcatttga ctgacccgac ctacaaaggt ccttatacta tcccaatttt aattgacaag 300

actgataatc gtattgtctg taaggaatct gcagatatgc ttcgactttt tactacagat 360

ttctctgatt tgcatcaaga ggatgctcct gtccttttca gccaagaaac tgcttcactt 420

attgataatg atattaaaga cattaataat aacttccaaa gcttaatgta taagttagct 480

tttcttgata aacaagcaga ttatgatact tatagtaagg aattcttcac ttttctagat 540

caaaaagaac acttactagg acaacgtcct ttcttgctcg gagataatct aagtgaggtt 600

gatattcatt tctttacacc attagtccgc tgggatattg ctgggcgtga tcttctacta 660

cttaatcaga aagcgctaga agactaccca aatatttttt cttgggctaa aactttatac 720

aatgacttta atttaaaaac attaaccaat ccacaatcca taaaaaataa ctattactta 780

ggcaaatttg gaagagctgt tcgtcatcat accatcgtac caacaggtcc aaatatggta 840

aaatgggaaa aataa 855

SeqID 100

atgaaaaaga aaattatttt gaaaagtagt gtccttggtt tagtcgctgg gacttctatt 60

atgttctcaa gcgcattcgc tgaccaagtc ggcgtccaag ttataggcgt caatgacttt 120

catggtgcac ttgacaatac tggaacagca aatatgcctg acggaaaagt tactaatgct 180

ggcactgctg ctcaattaga tgcttatata gatgatgctc aaaaagattt caaacaaact 240

aaccctaatg gtgaaagcat tagagttcaa gctggtgata tggttggagc aagtccagct 300

aactcagggc ttcttcaaga tgaaccaacc gttaaaacat ttaatgcaat gaatgttgag 360

tatggcacat taggtaacca tgaatttgat gaaggtttgg cagaatacaa tcgtatcgtt 420

actggaaagg cccctgctcc agattctaat ataaataata ttacgaaatc atacccacac 480

gaagctgcaa aacaagaaat tgtagtggca aacgttattg ataaagttaa caaacaaatc 540

ccttacaatt ggaaacctta cgctattaaa aatattcctg taaataacaa aagtgtgaac 600

gttggcttta tcggaatcgt taccaaagac atcccaaacc ttgtcttacg taaaaattat 660

gaacaatatg aatttttaga tgaagctgaa acaatcgtta aatacgccaa agaattacaa 720

gctaaaaatg tcaaggctat tgtagtcctt gctcatgtac ctgcaacaag caaggatgat 780

attgctgaag gtgaagcagc agaaatgatg aaaaaagtca atcaactctt ccctgaaaat 840

agcgtagata ttgtctttgc tggacacaat catcaatata caaatggtct tgttggtaaa 900

actcgcattg tacaagcgct ctctcaagga aaagcctatg ctgacgtacg tggtgtccta 960

gatactgata cacaagattt cattgaaacc ccttcagcta aagtagttgc agttgctcct 1020

ggtaaaaaaa caggtagtgc cgatattcaa gccattgttg accaagctaa tactatcgtt 1080

aaacaagtaa cagaagctaa aattggtact gccgaggtaa gtggcatgat tacgcgttct 1140

gttgatcaag ataatgttag tccagtaggc agcctcatca cagaggctca actagcaatt 1200

gctcgaaaaa gctggccaga tatcgatttt gccatgacaa ataatggtgg cattcgtgct 1260

gacttactca tcaaaccaga tggaacaatc acctggggag ctgcacaagc agttcaacct 1320

tttggtaata tcttacaagt cgtcgaaatt actggtagag atctttataa agcactcaac 1380

gaacaatacg accaaaaaca aaatttcttc cttcaaatag ctggtctgcg atacacttac 1440

acagataata aagagggcgg agaagaaaca ccatttaaag ttgtaaaagc ttataaatca 1500

aatggtgaag aaatcaatcc tgatgcaaaa tacaaattag ttatcaatga ctttttattc 1560

ggtggtggtg atggctttgc aagcttcaga aatgccaaac ttctaggagc cattaatccc 1620

gatacagagg tatttatggc ctatatcact gatttagaaa aagctggtaa aaaagtgagc 1680

gttccaaata ataaacctaa aatctatgtc actatgaaga tggttaatga aactattaca 1740

caaaatgatg gtacacatag cattattaag aaactttatt tagatcgaca aggaaatatt 1800

gtagcacaag agattgtatc agacacttta aaccaaacaa aatcaaaatc tacaaaaatc 1860

aaccctgtaa ctacaattca caaaaaacaa ttacaccaat ttacagctat taaccctatg 1920

agaaattatg gcaaaccatc aaactccact actgtaaaat caaaacaatt accaaaaaca 1980

aactctgaat atggacaatc attccttatg tctgtctttg gtgttggact tataggaatt 2040

gctttaaata caaagaaaaa acatatgaaa taa 2073

SeqID 101

atggcatata tttggtctta tttgaaaagg taccccaatt ggttatggct tgatttacta 60

ggagctatgc tttttgtgac ggttatccta ggaatgccca cagacttagc gggtatgatt 120

gataatggcg ttacaaaagg tgatcggact ggagtttatc tgtggacgtt catcatgttt 180

atatttgttg tactaggtat tattgggcgt attacgatgg cttacgcatc tagtcgctta 240

acgacaacaa tgattagaga tatgcgtaat gatatgtatg ctaagcttca agaatactcc 300

catcatgaat atgaacagat aggtgtatct tcactagtga cacgtatgac aagcgatact 360

tttgttttga tgcaatttga tgaaatgtct ttacgtttag gcctagtaac tcctatggta 420

atgattttta gcgtggttat gatactaatt acgagtccat ctttggcttg gcttgtagcg 480

gttgcgatgc ctcttttggt aggagtcgtt ttatatgtag ctataaaaac aaaaccttta 540

tctgaaagac aacagactat gcttgataaa atcaatcaat atgttcgtga aaatttaaca 600

gggttacgcg ttgttagagc ctttgcaaga gagaattttc aatcacaaaa atttcaagtc 660

gctaaccaac gttacacaga tacttcaact ggtcttttta aattaacagg gctaacagaa 720

ccacttttcg ttcaaattat tattgcaatg attgtggcta tcgtttggtt tgctttggat 780

cccttacaaa gaggtgctat taaaataggg gatttagttg cttttatcga atatagcttc 840

catgctctct tttcattttt gctatttgcc aatcttttta ctatgtatcc tcgtatggtg 900

gtatcaagcc atcgtattag agaggtgatg gatatgccaa tctctatcaa tcctaatgcc 960

gaaggtgtta cggatacgaa acttaaaggg catttagaat ttgataatgt aacattcgct 1020

tatccaggag aaacagagag tcccgttttg catgatattt cttttaaagc taagcctgga 1080

gaaacaattg cttttattgg ttcaacaggt tcaggaaaat cttctcttgt taatttgatt 1140

ccacgttttt atgatgtgac acttggaaaa atcttagtag atggagttga tgtaagagat 1200

tataacctta aatcacttcg ccaaaagatt ggatttatcc cccaaaaagc tcttttattt 1260

acagggacaa taggagagaa tttaaaatat ggaaaagctg atgctactat tgatgatctt 1320

agacaagcgg ttgatatttc tcaagctaaa gagtttattg agagtcacca agaagccttt 1380

gaaacgcatt tagctgaagg tgggagcaat ctttctgggg gtcaaaaaca acggttatct 1440

attgctaggg ctgttgttaa agatccagat ttatatattt ttgatgattc attttctgct 1500

ctcgattata agacagacgc tactttaaga gcgcgtctaa aagaagtaac cggtgattct 1560

acagttttga tagttgctca aagggtgggt acgattatgg atgctgatca gattattgtc 1620

cttgatgaag gcgaaattgt cggtcgtggt acccacgctc aattaataga aaataatgct 1680

atttatcgtg aaatcgctga gtcacaactg aagaaccaaa acttatcaga aggagagtga 1740

SeqID 102

atgaaaaaag gacaagtaaa tgatactaag caatcttact ctctacgtaa atataaattt 60

ggtttagcat cagtaatttt agggccattc ataatggtca caagtcctgt ttttgcggat 120

caaactacat cggttcaagt taataatcag acaggcacta gtgtggatgc taataattct 180

tccaatgaga caagtgcgtc aagtgtgatt acttccaata atgatagtgt tcaagcgtct 240

gataaagttg taaatagtca aaatacggca acaaaggaca ttactactcc tttagtagag 300

acaaagccaa tggtggaaaa aacattacct gaacaaggga attatgttta tagcaaagaa 360

accgaggtga aaaatacacc ttcaaaatca gccccagtag ctttctatgc aaagaaaggt 420

gataaagttt tctatgacca agtatttaat aaagataatg tgaaatggat ttcatataag 480

tctttttgtg gcgtacgtcg atacgcagct attgagtcac tagatccatc aggaggttca 540

gagactaaag cacctactcc tgtaacaaat tcaggaagca ataatcaaga gaaaatagca 600

acgcaaggaa attatacatt ttcacataaa gtagaagtaa aaaatgaagc taaggtagcg 660

agtccaactc aatttacatt ggacaaagga gacagaattt tttacgacca aatactaact 720

attgaaggaa atcagtggtt atcttataaa tcattcaatg gtgttcgtcg ttttgttttg 780

ctaggtaaag catcttcagt agaaaaaact gaagataaag aaaaagtgtc tcctcaacca 840

caagcccgta ttactaaaac tggtagactg actatttcta acgaaacaac tacaggtttt 900

gatattttaa ttacgaatat taaagatgat aacggtatcg ctgctgttaa ggtaccggtt 960

tggactgaac aaggagggca agatgatatt aaatggtata cagctgtaac tactggggat 1020

ggcaactaca aagtagctgt atcatttgct gaccataaga atgagaaggg tctttataat 1080

attcatttat actaccaaga agctagtggg acacttgtag gtgtaacagg aactaaagtg 1140

acagtagctg gaactaattc ttctcaagaa cctattgaaa atggtttagc aaagactggt 1200

gtttataata ttatcggaag tactgaagta aaaaatgaag ctaaaatatc aagtcagacc 1260

caatttactt tagaaaaagg tgacaaaata aattatgatc aagtattgac agcagatggt 1320

taccagtgga tttcttacaa atcttatagt ggtgttcgtc gctatattcc tgtgaaaaag 1380

ctaactacaa gtagtgaaaa agcgaaagat gaggcgacta aaccgactag ttatcccaac 1440

ttacctaaaa caggtaccta tacatttact aaaactgtag atgtgaagag tcaacctaaa 1500

gtatcaagtc cagtggaatt taattttcaa aagggtgaaa aaatacatta tgatcaagtg 1560

ttagtagtag atggtcatca gtggatttca tacaagagtt attccggtat tcgtcgctat 1620

attgaaattt aa 1632

SeqID 103

atggaaaatt ggaagtttgc cctatcatcg attctagggc acaagatgcg tgcttttctg 60

accatgttag ggatcatcat tggggttgca tcagttgtgt taattatggc cttaggtaag 120

ggcatgaaag attcggttac aaatgagatt acaaagtctc agaagaacct tcagatttat 180

tataaaacaa aagaagacaa aaaaaatgag gataactttg gagctcaagg ggctttcatg 240

caaggtagtg ataccaaccg taaggaacca attattcaag aatcatggtt aaaaaagatt 300

gctaaggaag tagatggtgt tagtggctac tatgtaacca atcaaacaaa tgctccagta 360

gcttaccttg aaaaaaaggc aaagacggtt aatataacag gagttaaccg cacttacctt 420

ggtatcaaaa agttcaaaat taaaagtggt cgtcaattcc aagaggagga ttataaccaa 480

ttctcgagag ttattttact agaagaaaaa ttagctcaga gattattcca gacaaatgaa 540

gcagcgttaa ataaggtggt gactgtaaaa aataaatctt acttagtagt gggggtttat 600

tcggatccag aagctggttc aggcttatat ggaagtaatt ctgatgggaa tgctatttta 660

accaatacac agttagcttc ggagtttggt gctaaagaag cagaaaacat atattttcat 720

cttaatgatg tatcgcaatc caatcgcatt ggtaaagaaa taggcaaacg tttaacagac 780

attagtcatg ctaaggatgg gtactatgat aattttgaca tgacatctat agttaaatct 840

atcaataccc aagttggtat tatgacaggt gttattggag cgattgcagc aatttctctc 900

ctagttggtg gtatcggagt gatgaatatt atgttagtct ctgtaactga gcgcacgaga 960

gaaattggcc ttcgtaaagc ccttggggca actcgacgta aaattttagc tcaattttta 1020

attgaatcaa tggtcttaac gattttgggt ggattaattg ggttattact agcctatgga 1080

ggtacgatgt taattgctaa tgcgcaagat aagattactc cttctgtgtc acttaacgtg 1140

gctataggca gtttaatttt ctcagcattt attggaatta tatttggttt attacctgct 1200

aataaagcca gcaaattaaa cccaattgat gctttacgat atgaataa 1248

SeqID 104

atgcagtatt cagaaattat gattcgttat ggagaactct ctactaagaa gaaaaaccgt 60

atgcgcttca tcaataagtt aaaaaataat atggagcatg tactctccat ttatccagat 120

gtttcagtaa aaacagatcg tgatagagga catgtatatc tcaatggtac agattatcat 180

gaagttgcag agtccttaaa agagattttt ggtatccaag ctttttctcc atcttttaaa 240

gtagaaaaaa atgttgatac attggtaaaa gctgtccagg aaattatgac ttccgtttat 300

aaagatggga tgacttttaa aattaccgca aaacgtagtg accactcatt tgaattggat 360

agccgtgctc taaatcatac tttaggagat gccgtttttt cagtcttgcc aaatattaag 420

gctcagatga agcaaccaga tatcaatctt aaagtcgaga tacgagatga ggctgcttat 480

atttcatatg agaatattag gggtgcagga ggattaccgg taggaacatc tggaaaaggg 540

atgctgatgt tgtctggtgg gattgattct ccggtggcag gttacctagc gttaaaacgt 600

ggtgtagata tagaagcagt tcattttgca agtcctcctt atactagccc aggtgcattg 660

aaaaaagcac atgatttaac acgtaaattg acaaaatttg gtggtaatat tcaatttatt 720

gaagttccat tcacagaaat tcaagaggaa attaaggaaa aagctcctga agcctacttg 780

atgacgttaa cacgtaggtt tatgatgcgt attacagatc gtattcgtga gaaccgaaat 840

ggtcttgtta ttattaacgg tgaaagttta gggcaggtgg caagccaaac gctagaaagt 900

atgcaagcca ttaatgctgt cactgcaaca ccgattattc gtcctgtggt cacgatggat 960

aagctagaaa ttattgatat tgctcaaaaa atagatactt ttgatatttc aattcaacca 1020

tttgaggatt gctgtacgat ttttgcacca gatcgcccaa aaactaaccc taaaattaag 1080

aatacagaac agtatgagaa acgtatggat gtagaaggtc tggtagagag ggcagttgca 1140

gggattatgg taactactat tcaacctcaa gcagatagtg atgatgttga tgacttgatt 1200

gacgatttat tataa 1215

SeqID 105

atggctcgtg ttaaaggtgg agttgtttca cgtaaacgtc gtaaacgcgt acttaaatta 60

gctaaaggtt actatggtgc aaaacatatc ttgttccgta ctgcaaaaga acaagtaatg 120

aattcttact actatgcata ccgtgaccgt cgtcagaaaa aacgtgactt ccgtaaatta 180

tggatcactc gtatcaatgc ggctgctcgt atgaatggtt tatcatattc gcaattgatg 240

cacggtttga aattagctga aatcgaagtt aaccgtaaaa tgcttgctga tttagcagtt 300

aacgatgcag cagctttcac agctcttgca gatgcagcta aagctaaact tggtaaataa 360

SeqID 106

atgtttaaag cttcaaaaaa actcgttcaa aaaaataagt caaatcattt ttggcttgtt 60

tttttcataa cacttatact atttcttatt ggttgttacg ccagcttacg atttggtgct 120

atcaatttca agactagtga tttaataact gttttaaaaa atcccttaaa aaactctaat 180

gctcaggatg ttatttttga cattagacta ccacgcataa ttgcagctat cttagtaggt 240

gcagctatgt cacaagcagg tgctattatg cagggagtca ctcgaaacgc cattgctgat 300

cctggactac taggtattaa cgccggagct gggttagccc ttgttgttgc atatgctttt 360

ttagggagca tgcactatag tacgattctc attgtctgct tactagggtc tgtcatttct 420

tatcttttag tcttcacact ctcttataca aagcaaaagg gttaccacca actacgactc 480

attttagctg gagcgatgat ctccacactt tttacgtctg ttggacaagt ggttacatta 540

tactttaaac tcaatcgaac tgttatcggt tggcaagcag gtggtttatc ccaaattaac 600

tggaaaatgc taatcataat agcacctatc attattttag gtttacttat ttcacaactc 660

ctagcacatc aattaactat cttgagcctt aatgaatctg tggccaaagc attgggacaa 720

aaaactcaat taatgactgc gtttctatta cttattgttt tgtttttatc ggcttcatca 780

gtcgcgttga taggaaccgt ttctttcatt ggattgatta ttccacattt tattaagctt 840

tttattccca aagattatcg tctgttatta cctttaatag gattttcagg ggctactttc 900

atgatctggg tagacttatc atctcgtatt ataaatcccc cttcagagac accaattagt 960

tctataatca gtatcgtagg gcttccatgt ttcctatggt taattagaaa agggaaaaat 1020

ctatga 1026

SeqID 107

atgattaaaa gaccaattca tctttctcat gattttttag cagaagttat tgataaagaa 60

gcaataacgc ttgatgcaac tatgggaaat gggaatgata ctgttttttt agcgaaaagt 120

tctaaaaaag tttatgcatt tgatattcaa gaagaagcaa tagcaaaaac gaaagcaaag 180

ctcacggagc aagggatttc taacgctgaa cttatcttgg atgggcatga aaatttggaa 240

cagtatgttc atacaccttt aagagcggct atatttaatc tgggatactt accctcagcg 300

gataaaacag ttattacaaa acctcacaca actatcaagg caatcaaaaa cgttttagac 360

attttagaag taggtggacg tctatcactt atggtttatt atggtcatga tggtggaaaa 420

agtgagaaag atgcggtgat agcattcgtg gaacaattac cacagaataa tttcgctaca 480

atgctttatc aaccactcaa tcaagttaat acgcccccct ttttaattat ggtagaaaag 540

ttacaatcat atgaaaatga ggtgtaa 567

SeqID 108

atgcgagtac atattacaag tatatatggg cagtcaccgc gtagcattgc ccttatatca 60

caaaaactag ttaaggatgt cggacgtcaa cttggttatg atgaaatggg aatctacttt 120

tataatgatc atgctgaaac gcatggtgaa agaagtacta ggatggatgg tatcattgcg 180

ggattaggca gaggggatat tgttgttttt caagtaccta catggaattc aacagaattt 240

gatgaacttt ttctagataa attacaggca tacggtgcta gaattataac ttttgtacat 300

gatattgttc ccttaatgtt tgagtctaat ttttatttat tagatagagt aattgacatg 360

tataatcgtt cggatgttgt tattctacct acaaaagcga tgcatgatta tctaattgaa 420

aaagggatga ctacctctaa agttctttat caagaagtat gggatcatcc agttaatata 480

gacctccctc gtccagaatg tcaaaaagtc cttagttttg caggcgatat tcaacgcttt 540

ccctttgtca atgactggaa ggaaaatatc ccgctcatct attatggaga cggaagcaga 600

ctcaattctg aggcaaatgt tcatgctcag ggatggaaag atgatgtaga attgatgcta 660

agtctttcta agcggggagg atttggcctg tgttggagtg aagatagaga ggagttagtc 720

gagcgtcgtt attcgaggat gaatgcctcg tataaattat ctactttctt ggctgctgga 780

cttccaatta ttgctaacca tgatatttca agtagagact ttatcaaaca acacggatta 840

ggatttacag ttgaaacgct agaagaagct gttgaaaaaa ttaataatat ggaaaaagag 900

acttatgaca gctatgtgga aaatgttgag aagatagcaa ctctcttgcg aaatggttat 960

atcactaaaa aattgttgat agatgctgtg catatgcttt atagatag 1008

SeqID 109

atgtcccaaa agacttttgg caagcagtta acagttgtag atactaagag tagagtcaag 60

atgcataaat caggaaaaaa ctgggtaaga acagtaatgt cgcattttaa tctatttaaa 120

gcgattaaag ggagagcaac tgttgaagca gatgtgtgta ttcaagatgt tgaaaaagaa 180

gaccgactat cttcaggaaa tttgacctat ctcaaaggaa tactagctgc cggagctctg 240

gtaggtggag cgagtttaac cagtcgtgtt tatgcagatg agactccagt tgttcaagaa 300

caatcaagtt ctgtaccaac actggcagaa caaacggaag tgactgttaa aacaactact 360

gttcaaaatc atcaagatgg gacagtatcg aaaaacatta ttgattctaa tagtgtatct 420

atgtcagagt cagcctcaac aagtactagt gaatctgtaa gtatgtctat gtcagggtca 480

actttaacaa gtgtaagtga atctgtaagt acatctgctt taacaagtgc ttcagaatcg 540

ataagcacgt cagcctcaga aagtgtttca aaatctacaa gtattagtga ggtttcaaat 600

attcttgaaa ctcaagcttc tttaactgat aaaggaagag agtcgttttc ggcaaaccag 660

atagtaacag aaagtagctt agttactgat gctggtaaaa atgcttcagt atctagccta 720

attgaaatta caaaaccaaa atcggagtta cagacttcca aaatgtcaaa tgagtcgctt 780

ataactccag agaaatccca agtaatgatt gcaagcgata aaactgggaa tgagagtcta 840

actccgacaa ttagattaaa atcagttatt cagccaagga gtatgaactt gatgactttg 900

agttcggaga tggacttgat accactagaa gaagtgtctg atactgaaat gttaggtaaa 960

gatgtatcaa gcgagttgca gaaagttaat attgcgttaa aagataacac tcttagtgag 1020

cctggaacag ttaaattaga tagttcagaa aaccttgttt tgaactttgc cttttcaatc 1080

gcttctgtta acgagggaga tgtctttact gtaaagcttt ctgataacct tgacacacaa 1140

gggattggta ctattctaaa agttcaagat ataatggatg aaacggggca gttattagcg 1200

actgggtcat atagtccttt aacacataat attacataca cctggacaag gtatgcttct 1260

acgttgaata atattaaagc tagagtcaat atgccagttt ggcctgacca gagaataatt 1320

tctaaaacaa cttcagataa gcagtgcttt actgcaacat tgaacaatca agttgcttca 1380

attgaggaac gtgttcagta taatagtcct tcagtgacag aacatactaa tgttaagaca 1440

aatgtaagat ctcggatcat gaagcttgat gatgaaagac agacagaaac ttatattact 1500

caaattaatc ctgaaggtaa ggaaatgtat ttcgcatcag gacttgggaa tctatatact 1560

attatcggtt cagatggaac atcaggttca ccagttaatt tattaaatgc ggaagtaaag 1620

attctaaaaa ctaattcaaa aaatcttaca gatagtatgg atcaaaatta tgattcgcct 1680

gagtttgaag atgtgacttc ccagtatagt tatactaacg atggttctaa aattaccata 1740

gattggaaaa caaattctat ttcttccact acatcttatg ttgttttggt caaaatacct 1800

aaacaaagtg gtgtattgta ttcaactgtt tctgatataa atcaaacata tggttctaaa 1860

tattcttatg ggcatacgaa tataagtggt gactcagatg cgaatgccga aattaaactt 1920

ttatcagaaa gtgcttctac gagtgcgtcg acgtcagcaa gtaccagcgc ttccatgagt 1980

gcctcgacat cagcaagtac cagcgcttcc atgagtgcct cgacatcagc aagtaccagc 2040

gcttccatga gtgcgtcgac gtcagccagc accagcgctt caaccagcac ctcaacgagt 2100

gcctcgacat cagccagcac aagtgcttca acaagtgcaa gtatgagtgc ttcaacaagt 2160

gcaagtacga gtgcatccac gtcagcaagt actagcgctt ccacaagtgc cagcaccagc 2220

gcctcaacga gtgcctcaac gtcagccagc actagcgcct caacgtcagc cagcaccagc 2280

gcctcaacca gtgcttcaac cagtgcttcc acatcagcca gcactagcgc ctcaatgagt 2340

gcgtcgatgt cagccagcac aagtgcttca atgagtgcct cgacgtcagc cagcactagc 2400

gcctcaacca gtgcttcaac cagtgcttca accagtgctt ccacctcagc aagtatgagc 2460

gcctcaacaa gtgcaagtac tagcgcttca acaagtgcaa gtatgagcgc ctcaaccagt 2520

gcaagtacta gtgcttcaac aagtgccagt accagtgcct cgacgtcaac aagtactagc 2580

gcttccacgt cagcaagtac cagtgcatcc acatcagcaa gtatgagcgc atccacgtca 2640

gccagcacca gcccctcaac cagtgcttca accagtgctt caaccagtgc ttcaaccagt 2700

gcttccacat cagccagcac tagcgcctca atgagtgcat ccacgtcagc aagtactagc 2760

gcctcaatgt cagccagtac tagcgcctca accagtgcca gtaccagtgc ttccacctca 2820

gcaagtatga gcgcctcaac aagtgcaagt actagcgctt ccacaagtgc aagtatgagc 2880

gcctcaacca gtgcaagtac tagtgcttca accagtgcca gtaccagtgc ctcgacgtca 2940

acaagtacta gcgcttccac gtcagcaagt accagtgcat ccacatcagc aagtatgagc 3000

gcatccacgt cagcaagtac cagtgcatcc acgtcagcaa gtatgagcgc ctccacaagt 3060

gcaagtatca gtgcatccac gtcagcaagt atgagcgctt ccacaagtgc aagtaccagt 3120

gcatcgacgt cagccagtac tagcgcctca atgagtgcat cgacgtcagc cagcaccagt 3180

gcttccacaa gtgcaagtac tagcgcctca atgagtgcat cgacatcagc aagtaccagc 3240

gcttccacaa gtgcaagtac gagtgcttcc acatcagcaa gtactagcgc ctcaacatcg 3300

gcaagtacaa gttcttccac aagtgcaagt accagtgcat cgacatcagc cagcactagc 3360

gcctcaatga gtgcctcgac gtcagccagc acaagtgctt ccatgagtgc gtcgacgtca 3420

gccagcacta gcgcctcaac gagtgcgtca atgtcagcca gcacaagttc ttcaacaagt 3480

gcctcgatgt cagccagcac tagcgcttca atgagtgcct cgacgtcagc tagcactagc 3540

gcctcaacga gtgcgtcaat gtcagccagc acaagttctt caacaagtgc ctcgatgtca 3600

gccagcacta gcgcttcaat gagtgcgtcg acgtcagcca gcaccagcgc ttcaacgagt 3660

gcgtcaatgt cagccagcac tagcgcttca atgagtgcca cgacgtcagc cagcaccagc 3720

gtctcaacga gtgcatcgac atcagcaagt accagcgctt ccacaagttc ttcaagctca 3780

gtgacttcta attcatcaaa agagaaggtg tattctgcct taccttctac gggtgaccaa 3840

gattattctg taactgctac tgccttaggt ttaggtttaa tgactggtgc aacccttttg 3900

ggacgaaaaa aatctaaaaa agataaagac taa 3933

SeqID 110

atgatagata gaaaagatac taaccgattt aaattagttt ccaaatatag cccttcggga 60

gatcaacctc aagctattga aacgttggtt gacaatattg aaggaggaga aaaagctcag 120

attttaaaag gtgctactgg tactggtaaa acctacacaa tgagccaagt tatcgcccaa 180

gttaacaagc caaccttggt gattgcacat aataaaacct tagcaggtca actttatgga 240

gagtttaaag aattttttcc tgacaatgcc gtagagtact ttgtttcata ttatgattat 300

tatcaaccag aagcttatgt gccttcttcg gatacttata ttgaaaaaga tagttcagtc 360

aatgacgaaa ttgacaaatt acgtcactca gcgacttctt cgttattaga aagaaatgac 420

gttattgttg tagcatctgt ttcttgtatt tacggtttag gttctcctaa agagtatgca 480

gacagtgttg ttagccttag gccaggtcaa gaaatttcac gtgatcagtt attgaacaat 540

ctcgttgata ttcaatttga gcgtaacgat attgattttc aacgtggaaa atttcgcgtt 600

cgtggcgatg ttgtagaagt ttttcctgcg agtcgtgatg agcatgcctt tcgaattgaa 660

ttttttggtg atgaaattga tcgtataaga gaaatagaat cgttaacagg gcgtgtgcta 720

ggagaagtgg aacatttagc tattttccca gcaacacact ttatgacaaa tgatgaacat 780

atggaagaag ctatttcaaa aatacaagct gaaatggaaa atcaagtaga actttttgaa 840

aaagaaggga aattgattga agcgcaacgt attcgacaaa ggacagaata tgatattgaa 900

atgcttcgtg aaatgggata tacaaatggt gttgagaact actcacgtca catggatggt 960

agaagtgaag gtgagccacc ttttactttg ttagatttct tcccagaaga ttttttgatt 1020

atgattgatg agagtcacat gacaatgggg caaatcaaag ggatgtataa tggagaccgt 1080

tcgcgtaaag aaatgttggt taattacggt ttccgtttac catctgcctt agataaccgt 1140

ccacttcgtc gtgaagaatt cgaaagccat gtccatcaaa ttgtctatgt atctgcaacg 1200

ccaggagact atgaaatgga gcaaacagat acagttgttg agcaaattat ccggccaaca 1260

ggacttcttg atccagaagt tgaagttaga ccaagtatgg ggcagatgga tgacttgctt 1320

ggtgaaatca atttgaggac tgaaaaaggt gagcgtacct ttattacaac attgacaaag 1380

cgtatggcag aagacttaac agattatctc aaagaaatgg gagttaaagt taagtatatg 1440

cacagcgata ttaaaacctt agaacgtaca gagattattc gtgatttacg acttggtgtg 1500

tttgatgttt taattggtat taatctctta cgtgaaggaa tagatgttcc tgaggtttcc 1560

cttgttgcaa tcttagatgc tgataaggaa ggtttccttc gtaatgagcg tggtcttatc 1620

caaacaatcg gacgtgcagc acgtaacagt aatggtcatg ttattatgta tgctgataaa 1680

ataacagatt ctatgcaaag agctatggat gaaaccgctc gccgtcgtcg tttacaaatg 1740

gattataatg aaaagcatgg aattgtgcca caaacaatta aaaaagaaat ccgtgattta 1800

attgctatta ctaaaagtaa tgacagcgat aagcctgaaa aagttgtcga ttacagtagt 1860

ttatctaaaa aagaaaggca agctgaaatt aaagctcttc aacagcaaat gcaagaagca 1920

gctgaattat tggactttga gctagctgca caaatccgag atgttatctt agaactaaaa 1980

gcaatcgatt aa 1992

SeqID 111

atgaagcaca agttaaaagc ttttacgctt gctttactct caatattctt tgtgtttggt 60

ggaaaggtca gtgcagaaac tgtgaatatt gtttctgata cagcatacgc tccattcgaa 120

tttaaagatt ctgatcaaac ttataaagga atcgatgttg acatcgttaa cgaagtcgct 180

aagcgtgctg gctggaatgt taacatgacg tacccaggtt ttgatgccgc agttaacgct 240

gttcaatctg gacaggcaga cgcgctaatg gccggaacta ctgttactga agcacgtaaa 300

aaagtcttta atttctcaga tacttattac gatacttccg ttattcttta tactaaaaat 360

aataataaag tcacaaacta caaacaacta aaaggaaaag tagtcggtgt aaaaaatgga 420

acagctgctc aaagcttctt agaggaaaat aaatctaaat acggctataa agttaaaaca 480

tttgatacaa gtgacctaat gaataacagc cttgattctg gttctattta cgccgctatg 540

gacgatcaac cagttgtgca atttgcgata aatcaaggaa aagcttacgc cattaacatg 600

gaaggcgagg cagttggtag ctttgcattt gctgtcaaaa aaggtagtgg acacgataac 660

ctaattaaag aatttaacac agcttttgca caaatgaaat cagatggcac ttataatgac 720

atcatggata aatggcttgg gaaagacgct acaaaaacaa gcggcaaagc aacaggtaat 780

gccaatgaaa aagcaactcc tgtaaagcca agttataaaa ttgtttctga ttcttcattc 840

gcaccattcg aatatcaaaa cggtaaaggg aaatatactg gttttgatat ggaattaatc 900

aagaaaattg ctaaacagca aggttttaaa cttgatatct caaatccagg ttttgatgcc 960

gctttaaatg ctgtccaatc tggacaagct gacggtgtta ttgcaggagc tacaatcaca 1020

gaagcacgcc aaaaaatctt tgatttttct gatccttatt acacatctag cgttatctta 1080

gcggttaaaa aaggaagcaa tgtcaaatca taccaagatt taaaaggaaa aacagttggt 1140

gctaaaaatg gtactgcctc atatacttgg ttatcagacc acgcagataa gtacaactat 1200

catgttaaag catttgatga agcatctaca atgtatgata gtatgaactc aggttcaatt 1260

gatgctctaa tggatgacga agcagttctt gcttacgcta ttaatcaagg tcgtaaattt 1320

gaaacaccta tcaaaggtga aaaatcaggc gatatcggat ttgcagtgaa aaaaggggca 1380

aatccagaat taattaaaat gtttaacaac ggtcttgctt cactcaaaaa atcgggtgag 1440

tacgataaac ttgttaaaaa atacctttcc acagccagca cctcttcaaa cgataaagct 1500

gctaaacctg tagatgaatc aactatttta gggttaattt ctaataacta caaacaattg 1560

ctatctggta ttggaactac tttaagttta actcttatct catttgcgat tgctatggtt 1620

attggtatta tctttggtat gatgagcgta tcaccaagta atactctccg cacaatttca 1680

atgatttttg ttgatattgt ccgtggtatt ccactcatga ttgtggccgc ttttattttc 1740

tggggtattc ctaatttaat cgaaagcatc acaggtcacc aaagtccaat taatgacttc 1800

gttgctgcta ctatcgctct ttctttaaat ggtggtgcgt acattgctga aattgtacgt 1860

ggtggtattg aagctgttcc ttctggtcaa atggaagcaa gtcgtagttt aggtatttct 1920

tacggcaaaa ctatgcaaaa ggttatcttg cctcaagcag tacgccttat gttaccaaac 1980

tttatcaacc aatttgtcat ctcattaaag gatacaacaa ttgtatcagc aatcggactt 2040

gtggaactct tccaaactgg taaaatcata attgcacgta actatcaatc attccgtatg 2100

tatgctatcc ttgcaattat gtatcttgta atcattaccc ttctcacccg tctagcaaaa 2160

cgtttagaaa agaggcttaa ataa 2184

SeqID 112

atgggagata aaccgatatc atttcgtgat aaagatggga attttgtttc agcggccgat 60

gtctggaatg ctgaaaaatt agaagaactc tttaacacat taaatcctaa tcgtaaactt 120

cgtttagaac gcgaaaaatt agcaaaagaa aaaggatag 159

SeqID 113

atggaaacat atactctatc gaatactctt aacattccta aaattggttt tgggacatgg 60

caactaactg aaggtgaaga agcttataag gcagtgactc atgctttaaa agttggttat 120

actcatattg atactgctca gatttatggc aacgaacaca gtgttggacg tgctataaga 180

gacagtggtt tggcgcgaga aagtatcttt ttgacaacta agatttggaa tgacaaacat 240

gactatcatt tagccaaagc ttctattgat gaatctctgc agaaattagg tgttgattat 300

attgatcttc ttttgattca ttggccaaat ccaaaagctc ttcgtgaaaa tgatgcgtgg 360

aaagctggga atgcaggcac ttggaaagcg atggaagaag cttataaaga aggaaaggta 420

aaagctattg gtgtttcgaa tttcatgaaa catcacttag aggctttatt tgaaactgcc 480

gaaataaaac ctatggttaa tcaaattatc ctagcaccag gttgtgctca agaggatttg 540

gttcgcttct gtaaaggaaa tgatatctta ttagaagctt acagtccatt tggaacaggt 600

gctatttttg aaaatgaatc tataaaagct attgctgaaa aatatggtaa atcagttgca 660

caagtagctc ttcgctggtc acttgataat ggctttttac cattacctaa gtcagcgact 720

cccaaaaata ttgaagcaaa ccttgatatt tttgattttc aattaaacga agatgatatt 780

actaccttaa tacaactaga ttcaggtatt aaacccaaag atccagataa tgtctcattt 840

taa 843

geqID 114

atggttaaag gtcaaggaaa cgttgtagca caaaataaaa aagcgcatca cgactatact 60

atcgttgaaa caatagaagc aggaattgtt ttgacaggaa cagaaatcaa aagtgtccgc 120

gcagcgcgta ttacattgaa agatggatat gcacaaataa aaaatggtga agcgtggttg 180

attaatgttc atattacacc atacgatcaa ggtaatatat ggaatcaaga tcctgatcgt 240

acacgaaagt tattactaaa aaaacgtgag attgaaaaaa tatcaaatga actgaaaggg 300

actggtatga ctctggtacc tctaaaagtt tacttaaaag atggttttgc taaagttcta 360

ttgggtttgg caaaaggaaa acatgattat gataagcgag agtctatcaa acgtcgtgag 420

caaaaccgtg atattgcccg tcagttaaaa aactataatt cacgatga 468

SeqID 115

atggaccatt tcacaaaact ctggcaagat ttctctaaac ttccaaatgt tgttgctata 60

gcactcggag gctcacgctc tggtgacagt tttgaccaat cttccgatta tgatttgtat 120

gtttattgtg cagcaactcc tgatatcact agtcgtaaac gtatccttaa caagcactgt 180

cattacattg aacttaacaa tcattactgg gagcttgaag ataatggtac tttaaacgac 240

ggaactgata ttgatattct ctatcgtaac atagataact ttttatcaga cttagaagat 300

gtcgttgaac accacaattc tcgaattgga tacactactt gtttttggca taacctcatc 360

aattgccaaa tactctatga tcctgaaaat caattacaat cactcaaaga gagattcgaa 420

gtttcttatc ccagtcagtt acaaaaacaa attatcattc aaaatcgtaa cttattaact 480

ggcaagcttc cctcttacga taaacaaatt ataaaagccc ttaaacgcca agactttgtt 540

agtactcacc atagaactac tgctttctta gattcctact ttgatattat ttttgcactt 600

aataagttga cacatcctgg cgaaaaaaga atgatttcct atgctaagaa gaatgctaca 660

ttgcttccta aacatttcga agaaaatatc attaaactat gtcatcacaa ctccaacgaa 720

cacactgtta aagaaacatt aaacgatata ataatgcatc tcgacgtcat gcttaaagaa 780

aattttcaac actttatagg ttaa 804

SeqID 116

atgaaaaaaa ttatttatct tggtttagcc tgtgtcagta tattaacttt aagtggttgc 60

gagtcaattg agcgttctct aaaaggagac cgttatgttg accaaaaact agctgaaaac 120

tcttcaaagg aagctactga acaactaaac aaaaaaacta agcaagcctt aaaagctgat 180

aaaaaagctt ttcctcaatt ggacaaggct gttgccaaaa acgaggcaca agtgcttata 240

aaaacctcta aaggtgatat aaatatcaag ttatttccaa aatatgctcc tttagctgtt 300

gaaaattttc taacacatgc aaaagaagga tactataatg ggttaagttt ccacagagtt 360

atcaaagatt ttatgataca atctggtgac cctaatggag acgggaccgg tggaaaatct 420

atatggaata gcaaagataa aaagaaagat tctggaaatg gttttgttaa tgaaatatcc 480

ccttacctct acaatatcag gggaagttta gcaatggcaa atgctggagc tgatactaat 540

ggcagccaat tctttatcaa tcaaagccag caagatcatt ccaaacaact gtcagataaa 600

aaagttccta aggtgattat taaagcctat tcagagggag gaaatccaag tttagatggt 660

ggttataccg tctttggaca agtaatctct ggcatggaaa ctgtggataa aattgcttcg 720

gtagaagtta caaaatcaga tcaaccaaaa gaaaaaatta ctattacaag tatcaaagtt 780

attaaagact acaaatttaa ataa 804

SeqID 117

atgaaaattg gaattattgc agccatggag gaagagttaa aactccttgt ggaaaatctt 60

gaggataaat ctcaagagac agtattatca aatgtatatt acagtggacg ttatggtgaa 120

catgagcttg ttttagtaca gtctggtgtt gggaaagtca tgtcagcgat gagtgttgct 180

attttagtag aaagctttaa agttgatgct attattaata caggatcagc tggtgcagta 240

gcaacaggat tgaatgtagg agacgtcgtt gtcgcagata cattagttta tcacgatgta 300

gatttaaccg cctttggtta tgactatgga caaatgtcaa tgcagccact ttattttcat 360

tcggataaga cttttgtttc cacttttgaa gcagttttat ctaaagaaga aatgactagt 420

aaagttggtt taattgcaac tggggatagt tttattgctg gccaggaaaa gattgatgtt 480

attaaggggc attttccaca ggttttagct gtcgagatgg aaggtgcagc aattgcccaa 540

gctgctcaag ctacaggaaa gccctttgtg gtggtacgtg ctatgagtga tactgcagct 600

catgatgcca atatcacatt tgatgaattt atcattgaag ctggtaaacg ctctgcccaa 660

gtattaatgg catttttgaa agcattataa 690

SeqID 118

atggaaaaga gacttagttt aggagctctt gttttagcta gtacagttct tttagcagca 60

tgtggaaatg tcggaggagg agcatcttcg acaggaacaa aaatagggaa agatattaag 120

gtagggtata actgggaatt atcaggaaac gtttcttcgt atggaaattc aatgaagaat 180

ggagctgact tagcagtaaa agaaattaat gctgctggag gggttggcgg caagaagtta 240

aaagtcctat cacaggataa taaatcagaa aatgcagagg cagcaacggt tgccacaaac 300

ttggttacca aaggagctaa tgtcattatc ggaccagcaa catcgggtgc agctgcatct 360

tcaactccaa aagtaaatgc agcagcagtt ccaatgattg cacctgctgc gacacaagac 420

aatttagtct atggttctga tggaaaaacc ttaaatcagt atttcttccg agctactttt 480

gtcgataatt atcaaggaaa gctattgtct cagtatgcta cagacaacct taaagctaaa 540

aaagttgttc tattttatga taattcatca gattactcaa agggggtagc aaaatcattt 600

aaggaaagtt atagtggaaa aattgttgat agcatgacat tctccgctgg tgatactgat 660

ttccaagcgt cattgactaa gttgaaaggg aaagaatatg atgctattgt gatgccaggt 720

tactataccg agacaggatt aatagttaag caagcgcgtg atttaggtat ctctaaaccg 780

gttcttgggc ctgatggttt tgatagtccg aaatttgtgc aatcggcaac acctgtggga 840

gcttcaaacg tttattattt gacaggtttc actacacaag gatcaaccaa agctaaagct 900

ttccatgatc attacgttaa ggcatatggt gaagaaccat ccatgttctc ggctttatca 960

tacgatgccg tgtatatggc agctaaatct gctaaaggag ctaaaacttc aattgatctt 1020

aaaaaagctt tagctaagct gaaagatttt aaaggtgtga cagggaaaat gtctattgat 1080

aaaaatcata atgttgttaa atcagcttac gttgtcaaat tagacgatgg aaaaacaagt 1140

agcgttaaca ttatttcagc aaaataa 1167

SeqID 119

atggagaatc ataattctat taaacagacc tatggattaa tgactacaat tgctatgatt 60

gtaggtgtag tgattggttc aggcatttat tttaaagttg atgacatcct taaatttact 120

ggtggagatg tctttttagg catggtaatt ttagtactag gttctttttc gattgttttt 180

ggaagtttgt caatttctga gctagctatt agaacgagtg aaagtggtgg tattttttct 240

tactacgaaa aatatgttag cccagcttta gcagcaactt taggcttgtt tgcgtctttt 300

ttgtatcttc caactttaac agcaatcgtt tcatgggtag ctgcatttta tactttaggt 360

gaatcatcaa gtttggaatc acagattatt ttagctgctg tttatatttt agcacttagt 420

ctcatgaata tctttgctaa gcgtattgca gggggattcc aatctttaac gacctttgta 480

aaaatgattc ctttagtcct aattgcttta attggtgctt tttggtctga taaagctcca 540

caacttccac aacatttaac agctattcag ccgtcaaatg ttggatggag ttgggtgtca 600

ggtctggtac cattgtattt tgcatatgat ggttggacaa tttttgttag tattgcacca 660

gaagtcaaga atcccaagaa gaatttacca cttgcctttg tcattggtcc agcgcttatc 720

ttattatcat atttggcatt tttctatggt ttgacacaaa ttttaggtgc tagctttatt 780

atgacaaccg gtaatgatgc tattaattat gcagctaaca ttattttcgg tcctagtgtt 840

gggcgtttat tatctttcat tgttatttta tctgtattag gtgttgccaa tggactttta 900

cttgggacga tgcgtctacc tcaagctttt gcggaacgcg gatggatcaa aagtgagagg 960

atggctaaca taaatttgaa gtatcaaatg tcattgccag ctagtttgac ggtgacggct 1020

gtagctattt tttggctttt tgttcacttt atggttacaa aatttaattt attgccgggt 1080

agtgatatta gtgaaattgc tgtggtgttt aataatacta gtttaatcat tctttatgtt 1140

ttagtactca gcctttatct caaaaaagat attaaaaata aatttaccgg cttagtttca 1200

ccgattctag caattttagg cggtttgatc ctctttatcg ggagtttgtt aagtaatttc 1260

tttacggttt taattttcca atgtttttgc ttattattct gtttgatttg tcattatatc 1320

tatcaaaaaa ataacccaaa aactcatgaa tag 1353

SeqID 120

ttgaccgaat ttaatgacga tcagcattct aaccatgatc agaaaagttt taaagaacaa 60

attctggcag agttagaaga agctaaccgt ttgagaaagt tgcgtgaaga agaactctat 120

caaaaagagc aagaagccaa agaagctgct cgcagaacag cacaattgat ggcagattat 180

gaagcgcaac gtttgaaaga cgaacaagaa gctagagcaa aggcgctaga gactaagcaa 240

cgcttagaag agcaagagaa ggctcggatt gaggcgaaac ttctggcaga agctgctcga 300

gaagaagaaa gacgtcaggc ggaacaagca ttagcttccc aagaagaaca agttatcaat 360

caagggatgg aaccttcaag agagcttgat agcggctcca aatctagtga atttaggaca 420

actgaaaatg tacctgacat agatttaaaa gcagataaga ctgatgttgc aactgctgtg 480

cctaatcaag aaacggaaga aatctttctt gtgagagcaa cagatattcc tacagaaggt 540

gaaaatgtaa aacttggtga gacatcagag ttagagcccg tagcaaagga acctatccga 600

gtagaggatt tgtcaaaaga agaagaggat atagctttgt cagccaagaa caaacataac 660

aagcgtgaaa gacgtcagaa agcagataat gtggcaaaac gaattgcacg tattctgata 720

tcaattatca tacttgtatt gctcttgaca gcatttgtag gttatcgttt tgtagatagt 780

gctattaaac ctgttgattc caattcaaat aaatttgtac aagttgaaat tccgatagga 840

tctgggaata aattaattgg tcaaatttta gaaaaagcag gagtcattaa gagcgccaca 900

gtctttaatt actattccaa gttcaaaaac tatagtaatt ttcaaagtgg atactacaat 960

cttaaaaaaa gtatgacttt agatcagatt gctgctgaat tggaaaaagg cggaactgca 1020

gaaccgacaa aaccagcttt aggtaagata ttaattaccg agggttacac tattaaacag 1080

attgctaaag ctattgagtc taataaaatt gatacaaaga ctacaagtac accttataaa 1140

gctgatgatt tcttgaaact tgttcaagat gaaacgttta ttaaaaaaat ggtagctaaa 1200

tatccaaatt tactaggaag tctacctgat aaatcaaaag ctatttatca attggaagga 1260

tatttattcc cagctactta caactattat aaagatacaa ccttagaagg acttgttgaa 1320

gatatgattt caacaatgaa tactaagatg gcaccttact ataacactat taaagcgaag 1380

aatatgtctg taaatgatgt tttgacatta tcatctcttg ttgaaaaaga gggatcaaca 1440

gacgaagatc gccgtaaaat agcaagtgtc ttttataacc gattatcagc cggtcaagct 1500

cttcaaagta acattgcaat tttgtatgct atggggaaat taggtgataa aacaagttta 1560

gctgaagatg ctcaaattaa tacttcaatt aaatcaccat acaacatcta tactaatact 1620

ggtttgatgc ctggaccagt tgatagtcca agtatctcag caattgaagc gactataaaa 1680

cctgcttcaa cagattatct ttattttgtc gctgatgtga agaccggaaa tgtatactac 1740

gctaaagact ttgaaacaca caaggctaac gttgaaaaat atatcaatag tcaaattaac 1800

taa 1803

SeqID 121

atgagtagaa tgattccagg acgtattcga aatcaaggta tagaactcta cgaacaaggt 60

ttggtgtcct tgatttctca agaaggtaat ctattaaaag ctaaagttgg ggattgtcag 120

attgaatatt cattagtaac agaagaaaca aaatgttctt gtgatttttt tgcaagaaaa 180

ggctattgtc aacatttagc tgctctggag catttcttga aaaacgaccc agagggtaaa 240

gcaatcttat ctaaagttca agttcaacaa gaaagtcaac aagaaactaa gaagaaaacc 300

tcttttggta gtgttttttt agatagtcta attattaatg aagatgatac tataaaatat 360

caactatcgg ctcaaggaga acaaaatcct tatgctaatg atatctggtg gactttaaaa 420

attagacgac tgcctgatga tagaagttat gttattcgtg acatcaaagc ttttttaaat 480

actgttcgaa aagaagctta ttatcagatt ggaaaacaat actttgaaac tttgtctttg 540

atacagtttg atgagaccag tcaagaattg atagaatttt tatggaggct cattccaagt 600

cattcaagca aaattgatct agagtttatt ttaccaaatc aaggtcgtca ccttagtctc 660

actcgtggtt tttttgaaga gggcgttact ttgatgaatg ctttagaaaa tttttcattt 720

gaaagtgatt ttcatcaatt taatcacctc tactttaagg aattagaagg tgaggatcat 780

ctttatcaat ttaaggttat tgtccatagg caatcaattg aattagaaat caaagagaaa 840

gacttaaaac cactatttgc gaattcttat ttgttttatc gcgatacttt ctaccatctt 900

aatttgaagc aagaaaagat ggtaacggct attcgtagtc ttcccattga gggagattta 960

gcaaaacata tccattttga tttagatgat caagataagt tagcagctca tttactggat 1020

tttaaggaga ttggactagt agatgctcca cggagcttct ctattcatga ttttaaggta 1080

aattttgagt tcgatattaa tagtcaaaat gaaattttat tgcaaatggt atttgattat 1140

ggtaatgatt taactgttca taatcgtcaa gaactagaac agttaacatt tgctagtcac 1200

ttcaaacatg aagaaaaaat atttaaactc ttagaaagat atggctttgc accccacttt 1260

tcaaccagcc atccagcata cagtgcacaa gaattatacg atttttatac ctatatgctt 1320

ccacaattta aaaaaatggg aacagtgagc ttatccgcaa aattggaaag ttatcgtctc 1380

atagaacgac cgcaaattga tattgaagct aagggaagcc tgctagatat ttcttttgat 1440

ttctctgatt tactggaaaa cgatgttgat caagctctgg tagctttgtt tgacaataat 1500

ccttattttg tgaataaatc agggcaatta gttatttttg atgaagagac taagaaagta 1560

agtgcaactt tgcaagggtt gagagctaga cgagcaaaga atgggcatat tgaattagat 1620

aatattgcag catttcaatt atcagaactg tttgcaaatc aagataatgt tagcttttca 1680

caacattttt accaattgat tgaagatttg agacacccag aaaagtttaa aatacctggt 1740

ctatctgttt cagcttcctt gcgcgattat cagcttacag gtgtccgttg gttaagtatg 1800

ttagatcatt atggctttgc agggatttta gctgatgata tggggcttgg aaaaacactt 1860

caaacgattt catttttaag tacaaaacta actagagatt ctcgtgtgct tattttatca 1920

ccatcaagtt taatttataa ttggcaagat gaattccaca aatttgcgcc agacgtagat 1980

gtagcagttg cctatggttc taagattcgt agagatgaaa ttattgctga acgtcatcaa 2040

gtaatcatta caagttattc atcatttagg caggattttg agacttattc tgaaggaaat 2100

tatgattatt taattttgga tgaagcacag gttatgaaaa atgctcaaac taaaatagcc 2160

catagcttga gatcttttga ggtcaaaaac tgttttgcat tatcgggaac acctattgag 2220

aataaactgt tagaaatttg gtccattttc cagatcattt tacctggttt attacctggt 2280

aagaaagagt ttttgaagtt gaaccctaaa caagttgctc gttatatcaa accatttgtg 2340

atgagacgtc gaaaagaaga agttctccca gaattaccgg atttgattga aatgaactat 2400

ccaaatgaaa tgacagacag tcaaaaagtt atctatttgg cgcaattacg ccaaattcaa 2460

gaaagtatcc aacattctag tgacgctgac ttaaatcgtc gtaaaattga aattttatct 2520

ggcatcacac gattacgtca aatttgcgac actccacgtc tttttatgga ttatgatggt 2580

gaaagcggta agttagaaag cctacatcaa cttttaacac aaattaagga aaatggacat 2640

cgtgctctga ttttttctca gttcaggggt atgttagata tcgcagaacg tgagatggta 2700

gctatggggc ttacaactta taaaattact ggttctacac cagcaaatga acgtcatgaa 2760

atgaccagag catttaatgc aggatcaaaa gatgctttct tgatttcttt aaaagctggt 2820

ggcgttggtc tcaaccttac aggagctgat accgttgttc tgattgactt gtggtggaat 2880

ccagctgttg aaatgcaggc gattagtcgc gcacataggt tggggcaaaa agagaatgtc 2940

gaggtttatc gcttgattac tagaggcaca atcgaagaaa aaattctaga aatgcaagaa 3000

actaaaaaac atttggttac aacagttctg gatggaaatg aaacccatgc tagcatgagt 3060

gtagatgata ttcgagagat acttggagtt tctaaataa 3099

SeqID 122

gtgttactta cagaaatcaa aaaatcacca gaggggcttt attttgataa aaaaatagat 60

atcaaagagt cgcttatgga gcgtcattca gagataatgg atatttctga tattcaagtt 120

tcaggtcatg tggtgtatga agatggtctc tatctacttg actataatat ggcttacgat 180

attacattac cgtctagtcg ttctatgaaa ccagttgttt tatctgaaaa gcaaactatt 240

aatgaagttt ttattgaagc tgaaaatgtt agcactaaaa aagagctcgt tgatcaggat 300

ttggtcttaa tattagagga agatgatatt aatttagaag aaagcgttat cgataatatc 360

ttgttaaata tacctcttcg tgttctggca gctgatgagg ttggggtaga agcggattta 420

tcaggtaaaa attggtcgtt aatgactgaa aagcagtatg aagaaaaaca agctaaagaa 480

aaagaaaaat ctaatccttt cgcagcctta gaaggtatgt ttgatagtga ttag 534

SeqID 123

atggaagtaa aaactgcaat agaatggatg cacactttta accaaaaaat tcaatccaac 60

aaggactatt tatccgaact agatactcca ataggtgatg gcgaccatgg tggcaatatg 120

gcgcgtggta tgactgctgt tattgaaaac cttgataaca atgaatttag tagtgctgcc 180

gatgttttca aaaccgtatc catgcaactt ttaagcaaag taggcggagc atctggacca 240

ctctatggtt ctgcatttat gggaattaca aaagctgaac agagtgaaag taccatttca 300

gaggcacttg gcgcaggact tgaaatgatt caaaaacgtg gtaaagctga acttaatgaa 360

aaaacaatgg ttgatgtttg gcacggtgtt attgaagcga ttgaaaaaaa tgaattgact 420

gaagaccgta ttgattcctt agtagatgct acaaaaggga tgaaagcaac aaaaggacgc 480

gcttcttatg ttggagaacg ctcattagga cacattgatc cagggtcatt ttcttcagga 540

ttattattta aagcattact agaagttgga ggtgtctaa 579

SeqID 124

atgcaagata aattaatgat aagaggagca agagctcaca atttaaaaaa tataagtgtc 60

gacatcccac gagacaagtt agttgtcgtt acgggactat caggatcagg taagtctagt 120

ttggcctttg acactatcta tgcagaagga caacgtcgtt acgttgaaag tctttctgct 180

tacgcgcgcc aatttttggg aaatatggaa aagcctgatg tagattctat tgatggcctt 240

agtccagcta tttctattga tcagaaaaca acaagtaaga atcctcgctc tactgttggg 300

actgtaacag aaattaacga ttatttacgt ttgctttatg ctcgtgttgg aaccccatat 360

tgtatcaatg gtcacggagc catcacagca tcttctgtag agcaaattgt ggataaggta 420

ctagcgcttc cagagcgaac aaagatgcaa attttagccc caattatacg tcgaaaaaaa 480

ggacaacaca aatcaacatt tgagaaaata caaaaagatg gttacgttcg tgttcgtatt 540

gatggtgata ttcacgatgt tactgaggtg cctgaattat caaaaagtaa gatgcacaat 600

attgatattg tagtagatcg attaattaat aaagagggca ttcgttcccg cttatttgat 660

tccgttgaag cggctctacg tttgtcggat ggttatgtcg ttattgatac aatggatggc 720

aatgagttat tgttttcaga gcattattct tgtcctgaat gtggttttac tgttcctgaa 780

ttagagccac gccttttttc ttttaatgct ccctttggtt cttgtacgac ctgtgatggc 840

ttaggaatta agttagaagt ggatatagat ttggttattc cagataaaag caagaccctt 900

agagaagggg ccttagttcc ttggaatcca atttcatcaa attattatcc aacgatgcta 960

gagcaagcta tgactcaatt tgatgttgat atggatacgc catttgaaaa attaagcaag 1020

gcggagcagg acctagccct atatggttca ggcgaacgag agttccattt ccattatatt 1080

aacgattttg gaggcgagag aaatattgat ttgccttttg aaggggttgt caataatatt 1140

aatcgtcgtt accatgaaac taatagcgat tacacacgca atgttatgcg agaatatatg 1200

aacgagctaa aatgtaacac ttgtcatggc tatcgtctca atgatcaagc actttgcgta 1260

agagttgggg gagaagaagg tcttaatatt gggcaagtct cagatttatc gatagcagac 1320

catttagaac ttttagaaac gttgcgttta tcttcaaatg aacaattaat tgctcgacca 1380

attatcaaag aaattcatga ccgcctaagt ttcttaaata atgttgggct taattattta 1440

aatctgtcac gttctgcagg aacgctttcc gggggcgaga gtcaacgtat tcgtttggct 1500

actcaaattg ggtctaattt atcaggtgtc ctttatgtac ttgatgaacc gtccattgga 1560

cttcatcagc gagacaatga ccgtttgatt gatagtctta agaaaatgcg tgatttgggt 1620

aatacgctta ttgttgttga acatgatgaa gatacaatga tggcagctga ttggttaatc 1680

gatgtgggac ctggcgctgg tgcgtttggt ggtgagattg tggcctctgg cacaccgaag 1740

caagtagcta aaaatactaa atctattaca ggacaatatt tgtcaggtaa gaaagtgatt 1800

cctgtgccat ctgaacgaag agtaggcaat ggtcgctttt tagaaatcaa aggtgcggca 1860

gaaaacaacc tacaaaattt agatgtcaag ttccctctcg gtaaatttat tgccgtaact 1920

ggtgtctcag gttctggaaa atcaacctta attaatagta ttttgaaaaa agctgttgcg 1980

cagaagctta accgtaactc agataaacca ggtaaatatg tttctttaga aggcattgaa 2040

tatgttgatc gattgattga tattgaccaa agtccaattg gtcggacccc acgttctaat 2100

cccgcaacct atacaggtgt ttttgatgat attcgtgatc tttttgctca aactaatgag 2160

gcaaaaattc gaggttataa aaaaggacgc ttctcattta acgtcaaagg tggacgttgt 2220

gagtcttgtt ctggtgatgg tatcatcaaa attgagatgc attttttacc agatgtctac 2280

gttccttgtg aagtatgcca cggcacgcgc tataatagtg agactctgga agtacattac 2340

aaagaaaaaa atattgcaca aattcttgat atgacagtga atgatgcagt tacatttttt 2400

gcagctattc ctaaaattgc tcgtaagtta cagactatta aggatgttgg tttgggatat 2460

gtcactttgg ggcaaccagc tacaacctta tcaggaggag aagctcagcg aatgaagttg 2520

gcaagtgaat tacataagcg ttcgacaggt aagagtcttt acatattaga cgaaccaaca 2580

acggggcttc atgcagatga tattgctcgc ttacttaagg ttttggatcg ttttgtagat 2640

gatggtaata cagtgcttgt tattgagcat aaccttgatg ttattaaaac tgctgaccac 2700

attattgatc tcggccctga aggaggtatt ggtggcggac aaattgtagc tatcggaaca 2760

ccagaagaag ttgctgaaaa tcctaaatcc tatacaggat attatttaaa agagaagtta 2820

gcaagatag 2829

SeqID 125

atgtggcctg aagatagaat cgcttctttt cgtcgcacac ttttagggtg gtatgaccaa 60

gaaaaacgtg atctcccttg gcgaagaact actaatccat actatatttg ggtttctgaa 120

atcatgttgc aacaaacaca agttaataca gtaatccctt attataaacg ttttctagag 180

tggtttcctc aaataaaaga tttggcagac gctcctgaag aacaattact aaaggcatgg 240

gagggactag gttattattc tcgggtgcgt aatatgcaaa aggctgcaca gcaagtgatg 300

gttgattttg gtggcatatt tcctcacact tatgatgata ttgcttcatt aaaagggatt 360

ggtccttata ctgcaggtgc tatcgccagc atctcattca acttaccaga gcctgccgtt 420

gatggcaatg tgatgcgtgt catggctcgt ttgtttgaag ttaattatga catcggtgac 480

cctaaaaatc gtaaaatttt tcaagctatt atggaaatac taattgaccc tgacagaccc 540

ggtgatttta accaagcact tatggatctg ggtacagaca tcgaatccgc taagacacct 600

agacctgatg aaagccctat tcgtttcttt aatgctgctt atttgaatgg tacttatagt 660

aaatatccca taaaaaatcc taagaaaaag cctaaaccaa tgcgaattca ggcttttgtc 720

attcgtaacc aaaatggcca atatctactg gagaaaaata ctaaagggag acttctaggg 780

ggtttttggt catttcctat tatcgaaacg tcacctcttt ctcaacaatt agatttattt 840

gatgacaatc aatccaaccc cataatctgg caaacccaaa atgaaacttt ccaaagagaa 900

tatcaattaa agccccaatg gactgataat cactttccca atattaagca cacttttagt 960

caccagaagt ggacaataga actcatagag ggggtggtca aagcaacaga tttacctaat 1020

gctcctcacc taaagtgggc agcaattgag gacttttctc tgtatccctt tgcaacgcca 1080

caaaagaaaa tgctggagac ctacttaaaa cagaaaaatg cttaa 1125

SeqID 126

ttgatcgaaa tcacttggac tgtgaagtat atcacagaat ttatcgcaac cgcttttctt 60

atcatcttag gaaatggggc tgtagctaac gttgacttaa aaggcacaaa aggaaataat 120

tcaggttgga ttattattgc aattggttat ggtttaggtg ttatgatgcc agcgcttatg 180

tttggtaatg tctctggaaa ccatattaac ccagctttca cacttggatt ggcattttca 240

ggtcttttcc cttgggcaca cgttggacaa tacattcttg cacaaatttt gggagcaatg 300

tttggacagt tagttgtcgt tatggtttat caaccttact ttgttaagac agaaaatcca 360

aatcatgttc ttggatcatt ttcaactatt tctgcattag atgatggaca gaaatcaagt 420

cgtaaagcgg cttacattaa tggtttctta aatgaattcg ttggttcttt tgttcttttc 480

tttggagctt tggctttaac gaagaactat ttcggtgtag aacttgttgg taaacttgtt 540

caagctggtt atgaccaaac gacagcagcg acaaggattt caccatacgt aactggttca 600

cttgctgtag ctcacttagg tattggtttc ttggtaatga ctctagttgc ttctttaggt 660

ggaccgactg gacctgcatt gaaccctgcg cgtgaccttg gtccacgtat tgttcaccgc 720

ttattaccaa aacaaattct tggacaagct aaagaagatt caaaatggtg gtatgcatgg 780

gtgccagttt tagcaccaat cgtagcaagt atactcgctg tagccttgtt caaactatta 840

tatctttaa 849

SeqID 127

atgtcatctt attggaataa ctatcctgaa cttaaaaaaa atattgatga aaccaatcaa 60

ctaattcaag aaagaataca ggtcagaaat aaagatattg aagcggcgct aagccaactc 120

acagctgcgg gaggaaaaca gctcagacca gcattctttt accttttttc tcaacttggt 180

aataaggaga atcaagatac tcagcaacta aagaaaatcg ctgcttcttt agaaatcctt 240

cacgttgcta cattaatcca tgatgatgtc attgatgact caccactaag acgtggaaat 300

atgaccattc aaagcaagtt tggcaaagac atcgcagttt atactgggga tttacttttc 360

acagtctttt tcgatcttat tttagaatct atggctgata caccatttat gaggattaat 420

gcaaaatcta tgcgtaaaat tctcatggga gaattggacc agatgcacct tcgttacaat 480

caacaacaag gtatccatca ctatttacgt gcgatttcag gtaagacagc cgaactcttt 540

aaattagcta gcaaagaagg agcttacttt ggtggtgcag agaaggaggt tgttcgtcta 600

gcaggccata tcggctttaa cattggtatg acattccaaa ttttggatga tatcctggat 660

tatactgcag ataaaaaaac atttaataag cctgtcttag aggatttaac acaaggcgtt 720

tacagccttc ctctacttct tgccattgaa gaaaatcctg atattttcaa acctatttta 780

gataaaaaaa cagatatggc tactgaagac atggaaaaaa ttgcttatct cgtcgtttcc 840

catagaggtg ttgacaaagc tcgccatcta gctcgtaaat ttactgagaa agctattagt 900

gacataaata agctacccca gaactctgca aaaaaacagt tgctacaatt aactaattac 960

cttttaaaac gcaaaattta a 981

SeqID 128

atgtttaaaa tccctttgtt taaagaatta aagacagatc aatgggtaaa accatttttc 60

aaacaatata aagtttcctt agtaattgct cttttccttg gttttatgac tttcttttct 120

gccagtgctc ttatgtttaa ttcgggctat ctgattagta agtcagcttc actaccgagt 180

aatattcttc tagtatatgt tccaattgta ctaacacgtg cttttggtat tggacgtcct 240

gtttttcgtt atatagaacg cctaacgagt cacaattggg tattacgaat gaattcacaa 300

ctgcgcttga agctttacca ttccttagaa agcaatgcta tctttatgaa gcgtgatttt 360

cgtttaggag atgttatggg gcttttagcg gaggatatta attatttgca aaatttgtat 420

cttaggacaa tatttccaac gattatagcg tggttattat atagctttat tattattgca 480

acaggcttct tttctctatg gtttgcttta atgatgttgc tttatttagc tattatgatt 540

tttctttttc cactttggtc gattttagct aatggtgctc gtcagactcg tgaaaaagaa 600

ttaaaaaatc atctttatac tgatttgacg gataatgtct taggtatttc agactggatt 660

tttagtcaac gtggtcaaga gtacgtcgcc ttgcatgaac gttcggaaag tgaactaatg 720

gctattcaga aaaaaattcg ttcctttaat aatcgtagag ctcttattgt ggaattagtt 780

tttggctttc tagctatttt ggttattatt tgggctagta atcagtttat tggtcacaga 840

ggtggcgaag ctaactggat tgcagcattt gttctaacag tatttccctt gtcagaggca 900

tttgctggtt tgtctgctgc tgctcaagaa acaaataaat attctgattc aattcatcgt 960

cttaatgaat tatcagaaac gtattttgaa acaactcaaa atcaattgcc taataagcca 1020

tatgattttt ccgtcaagaa tttaagtttc cagtataagc cacaagagaa atgggttttg 1080

catcatttag atttggatat taaagaagga gaaaaaattg ctattttagg gcggagtggt 1140

tctggcaaga gtaccttagc tagtctactt cgtggcgact tgaaagcaag tcaagggaag 1200

attaccctag gcggtgctga tgtttcaata gttggtgatt gcatttctaa ctatattgga 1260

gtgatacaac aagcgcccta cctttttaat acaaccctgc taaataacat tcgcattgga 1320

aaccaagatg ctagtgaaga agatgtatgg aaagttcttg aacgagttgg attgaaagag 1380

atggtcactg atttatcaga tggtctctat acaatggttg atgaagctgg acttaggttt 1440

tcaggtggcg aaaggcatag aattgctttg gctagaatct tactaaaaga tgtcccaatt 1500

gttatcttag atgagccaac tgttggactt gatcctatta ctgaacaagc cttactaagg 1560

gtatttatga aagaattgga aggtaagacc ttagtttgga ttacacatca cttaaaaggt 1620

attgaacatg ctgatcgtat cttatttata gaaaatgggc aattagagtt ggaaggttct 1680

cctcaagaat tgtctcaaag tagtcaacgt taccgtcagc tgaaagccgc tgatgatggt 1740

gatctatga 1749

SeqID 129

atgaaaaaac ttattacaga aaaaaaagtc aacaacgtct caactgttaa ctatttgaaa 60

ctcggtcttg tctcagcaat gtttgctgga ggagcattcg tagctcttgg ttctacacaa 120

ggtgtttcag cttcaacttt cacagcaccg caagcaacac atccaaaagc agaacgtcag 180

ttaacagatt cagaaatcta tgaacgtgcc caaaaacaag tgttaccaaa atatatccaa 240

ggttctctct caggtatcct taaccaacat tcaacacttt acaaacaaca aaacgcagca 300

gtgacaccgc aagtatcaag ccccaaagca gaacgtcagt taacagattc agaaatctat 360

gaacgtgccc aaaaacaagt attgccaaaa tatattcaag gttctctctc aggtatcctt 420

aaccaacatt caactttgaa tgcttaa 447

SeqID 130

atggaggaaa acatgaacat aaaacaatta aagtctaaaa caatgcttgg aacagtggct 60

ttggtgtccg ctttttcatt tgcttcaaca aatgcagatg cgaatacata taactatgca 120

gtagatgtag actacctagc aagtgcggag gaaattgctc aagcacatcc agctagtaat 180

acgtttccac taggtcaatg cacatggggt gtaaaggaga tggcaacatg ggcaggtaac 240

tggtggggaa acggtggtga ttgggctgcg agcgccgcat cagctggtta tacagtagga 300

acacaacctc gcgtgggttc tatcgtatgt tggactgatg gtagttatgg acatgtggct 360

tatgttacag ctgtagatcc tgtcactaat aaaattcagg ttttagaatc taattatgca 420

ggtcatcaat ggattgataa ttatcgtggt tggtttgatc cacaaaatac agcgacacca 480

ggggtagtta gctacattta tccgaattaa 510

SeqID 131

atgaaaatta accaaatgaa aaaagacgag ctttttgagg ggttttacct tataaagaaa 60

gcagaagttc gtaaaacgcg tgcaggtaaa gactttattg cctttacctt tcgagatgat 120

actggcgaaa tttcaggaaa tatgtgggat gcacaaacct ataatgtaga agagtttgta 180

gccggtaaaa ttgttcatat gaaagggcgt cgtgaagttt ataatggaac tccccaagtc 240

aaccaaatta cattaagaaa catcaaagat ggtgaaccta atgatcctag agattttaaa 300

gaaaaacctc caatcaatgt tgacaacgtg agagagtaca tggaacaaat gctctttaaa 360

attgaaaatg caacctggca gcgtgtggta agagcacttt atcgtaaata caataaggaa 420

ttttttacct atccagccgc caaaacaaac caccacgcct ttgaatcagg attggcatat 480

cacacggcaa cgatggttcg tttggcagat agtattggag atatctatcc agaacttaat 540

aaaagtttga tgtttgctgg tattatgcta catgatttag ccaaggtcat tgagttatcg 600

ggtcctgata atacagaata tactattcga ggtaacctta tcggtcatat ttcacttatt 660

gatgaggaat taacaaaaat actagcagag cttaatattg atgatacaaa agaagaggtt 720

acagtgttac gccatgttat tctgagtcac catggacaat tagaatatgg aagcccggtt 780

cgtcctagaa tcatggaggc agaaattatc catatgatcg ataacattga tgcaaatatg 840

atgatgatga caacagcttt aaatcgtgtc aacgaaggtg aaatgacaaa tcgcattttt 900

gccatggata accgttcttt ttataaaccg aacattaaat aa 942

SeqID 132

atgttggttg acaagaagtg gcgttttgaa gatagtgcaa gttatttcgc ttgtcctaaa 60

tgtcaaaatc ccttgataaa agaaagtaac agcctcaaat gctctgataa tcattgtttt 120

gatttatcca aatttggata tgttaatctt cttggaggaa agaagataga tgaacattat 180

gataaaaaat catttgaaaa tcggcagctt gtcttagaaa acgggtatta taatcatatt 240

ttagaagcta tttcaaaggt tttggaaaat aattctcagt ttcattcggt tttagatatt 300

ggttgtggag agggctttta ttctcgtcaa ttagttaaca agtatgagaa gactttcttg 360

gcttttgata tttctaaaga ttcgattcaa ttagctgcta aaagtgatca atctaggctt 420

gtgaaatggt ttgtttctga tttagctaac cttcctattc aagactcatc aattgatatc 480

attttagaca ttttttctcc tgcaaattat aaagaattta ggcgggtact gagtgatgat 540

ggtatccttg taaaggttgt tcctgtagct gaacatgtcc aagaattgcg agaaaaagct 600

agtcaatact tgaaacagaa agattactct aatcaaaaaa ttctagatca ctttagagaa 660

aattttgaaa ttatttctga gcagaaagtt gttcaaagtt ataactgttc tcaacaagaa 720

cgtcaagctt ttattgatat gacaccgttg ttattcagcg tagataaaac gactattgat 780

tgggcgagta tatcagaaat tacagtaggt gccttaatag taattggaaa aaagcgcagt 840

gtatctaaat aa 852

SeqID 133

atgtgtctta tttgtcaacg tattgaaatg attgaacgaa atgagaatcc ttattttgtg 60

aaagaatatg aaacgggtta tcttgttctg ggagaccatc aatattttca aggttattgc 120

ctttttctct ctaaaaaaca cgtaacagag ttacatgaat tgcctagaga ttggcgaaat 180

cagtatcttt ccgaaatggc agatgcttct gaaatagttg ctaaggcttt tagagcagat 240

aagatgaata ttgagagttt gggaaatgga gacgctcatc tacattttca tctttttcca 300

agaaaaacag gtgatttaag gaactatggg cataatggaa aaggtccagt ttggtggtat 360

ccttttgaga agatgtatgc agatagtgtc agagcaactg gtgcagaaat agaaaaatta 420

aaagagaaat tgttagatgt cttaggatag 450

SeqID 134

atgtttagtg atttacgcaa aaaatttgtc tttttgacca tgtcaatcct tattgtcgtt 60

gttctctttc tctttgcagt gagtaatcgc tacaatcaat attgggatga atatgatgca 120

tataggattg ttaaattagt tgccaaaaat gattatttag gaataccagg cgatgaaccg 180

attgctttgg tgactataga taaccaaaag atggttaaga ttcaaagcaa taatactgat 240

ttgacaaatg atgttatcga aaaatctagt ttgaagctcc ttgagcaagg aaagaagtca 300

cgaaaatgga agtcttttat atattccata aaagaatata aagataaaac ctacaccata 360

gctattatgg atttggctag ttatgaagtc ccttatgcaa ggcgttttct aattcttgtt 420

tttactattt ttggattttg tttattagca gctgtaagtc tttatctttc tcgatttatc 480

gtagggcctg tagagacaga aatgactcgc gaaaaacagt tcgtttcaga tgcgagtcat 540

gagctgaaga cccctattgc agctatacga gccaacgttc aggttctgga acagcaaatt 600

ccaggaaatc gttatctgga tcatgttgtt tcggagacta agcgaatgga gtttttaatt 660

gaagatttgc ttaatttgtc tagacttgat gaaaaacgct ccaaggtaaa tttcaaaaag 720

ttaaacttat ctgtcttatg tcaagaagta cttctgacat atgaaagttt ggcatatgaa 780

gaagaaaagt gtttaaatga tacgattgaa gatgatgtct ggattgttgg agaagagagt 840

cagattaagc aaattttaat aattcttttg gataatgcta ttaggcattc tttgtcaaaa 900

tcagaaatcc agtttagtct taaacaggct cgaagaaaag ctattctgac aatatcaaat 960

ccaagtgcta tctattccaa agaggttatg gataatctct ttgagcgttt ttatcaagct 1020

aaagatgatc atgcagattc tctcagtttt ggtctaggct tatctattgc taaggcaatc 1080

gtggaacgcc ataaggggag aattagagca tatcaagaaa aagaccaatt acgtcttgaa 1140

gtccagttac caattgatgg attttggacc aataccatga ttaattaa 1188

SeqID 135

ttgattattc tcgataaaaa aagttatgac ctcctctttt acctattgaa attagaggaa 60

cctgaaacag ttatggcaat tgccaacgca cttaatcagt ctaggcgtaa agtgtattat 120

cacttagaga agataaacga tgcactgcct agcgatgtgc ctcagattgt tagttatcca 180

cgtgtaggaa tcttgctaac cgaaaaacaa aaagcagcct gtcgtctttt attagatgaa 240

gtaactgatt atagttacgt catgaaaagt agtgagaggt tgcagctgtc tttagtatct 300

atcgtggtag ctaaggaccg cataacgatt gataggttga tgcaactaaa cgatgtttct 360

cgaaatacta tcttaaacga tttaaacgaa ttaagaagtg agttagcaga gaaagaatat 420

aatttacagt tacaatcaac aaaatgtcgc ggttattttt tagatggtca cccattgtcc 480

attatccagt acttatataa gctcttagat gatatctacc ataatggaag tagtagtttt 540

atagaccttt ttaatcataa actgtctcaa gcttttggtg ccagcacata tttttctaaa 600

gaggttcttg attattttca ccattatctc ttcatttctc aacgaagtct aggtaagaaa 660

atcaacagtc aagatggtca gtttatgatt cagattttgc cttttatact aatggcttat 720

cgtaagatgc gattaagtcc tgaagtacag acctctctta atagtgattt tagcttggtt 780

tggcaacgta aggaatatga gattgctaaa gagttggctg atgagctgga agaaaatttt 840

cagttatcac tggatgagat tgaagtggga ctagtggcca tgcttatgct tagtttccgc 900

aaggaccgtg acaatcattt agagagccag gattatgatg atatgcgagc tactctaacc 960

agttttttga aagaattgga agaacgatat caccttcact ttgttcataa aaaggactta 1020

ctaagacaac ttctaactca ctgcaaggca ctcttatatc gtaaacgtta tggtattttt 1080

tctgttaatc ctttaacaga gcatattaaa gacaaatatg aagaactttt tgccataacc 1140

tcgtcttctg taaagctttt agagaaagct tggcaaatca aattgaccga tgatgatgta 1200

gcatatctaa cgattcattt aggaggggaa cttcgtaata gtcaacaatc tcctaataaa 1260

cttaagttag ttattgtatc tgatgaagga atagcgattc agaaacttct tttaaagcaa 1320

tgtcaacgct acttaacaaa cagtgatata gaagctgttt ttacaaccga acagtaccaa 1380

agtgtgagtg atcttatgca tgtagatatg gttgtctcta ctagtgacgc tttagaatct 1440

cgttttccga tgttagtagt tcaccctgtt ttgacagatg atgatattat tcgcttgatt 1500

cgcttttcaa aaaaaggtaa ctgtgcaaat agtaatcaat ttaccaatga acttgaaaaa 1560

acgattgctc aatatgtcaa ggaagatagt gaacgctacg tgctgaaatc taagattgag 1620

aaacttattc atcaagaatt gctccaagac gtccttcccc ttcaaagtac agtttgttaa 1680

SeqID 136

atgaaatact ttttagatac tgccgatgtt tctgaaattc gtcgtcttaa tcgtttagga 60

atcgttgatg gtgttactac caatccgact attatttcac gtgaggggcg tgactttaaa 120

gaagttatta acgaaatttg ccaaatcgtt gatggcccag ttagtgcaga agtgacaggt 180

ttaacttgtg atgaaatggt taccgaagct agagaaatcg ctaagtggtc accaaatgtt 240

gttgttaaaa ttccaatgac tgaagaaggt ttagcagcag tttcacaatt atcaaaagaa 300

ggtataaaga caaatgtgac attaatattt actgtggctc aaggtctttc ggcaatgaaa 360

gctggtgcaa catttattag tccttttgtg ggtcgtcttg aagatattgg aacagatgct 420

tatgctttga ttcgtgacct tcgtcacatt attgattttt atggtttcca atctgagatt 480

attgctgcta gcattcgtgg attggcgcat gtggagggcg tcgctaaatg cggtgcgcac 540

attgccacta ttccagataa aacatttgca tcactattta ctcacccgct tactgataaa 600

gggattgaaa ctttcttaaa agattgggat agtttcaaga agaagtag 648

SeqID 137

atgagtcatt attccataaa attacaagaa gtattcagat tggcgcaatt tcaagcagca 60

cgctatgaaa gtcactattt agaaagctgg catcttcttt tagcaatggt tttagttcat 120

gattcagtag ctggtttgac atttgctgaa tatgagtctg aagttgcaat tgaagagtac 180

gaagcagcaa caattttagc tttaggtaga gcaccgaaag aagagattac caattatcaa 240

tttttagagc aatcaccagc tttgaaaaag atcttaaagt tagcagaaaa tattagtatc 300

gtagtaggag ctgaagatgt gggtactgaa catgtcttgc ttgctatgtt ggttaataaa 360

gatttattag ccacacgtat cttagagtta gttggcttta gaggtcaaga cgatggtgaa 420

tcagttcgta tggttgattt acgtaaggct ctagaacgtc atgccggttt tacaaaagat 480

gatatcaaag caatttatga attacgtaat cctaaaaaag caaagtcggg tgcttctttt 540

tctgatatga tgaaaccacc gagtactgct ggtgatttag ctgatttcac acgtgattta 600

tcacagatgg cggttgatgg ggaaattgaa cctgttattg ggcgtgataa agaaatttca 660

cgtatggtcc aagtcttaag tcgtaaaaca aagaataatc ctgtcttagt tggagatgcg 720

ggtgtcggta aaacagcttt ggcgtacggt ctagcacaac gtattgctaa tggaaatatt 780

ccttatgagc ttagagatat gcgtgttcta gagttagata tgatgagcgt tgttgctgga 840

actcgttttc gtggtgattt tgaggaacgt atgaatcaaa tcattgcaga tattgaagaa 900

gacggtcata ttattctttt cattgatgaa ctgcatacta ttatgggttc tggctctggt 960

attgatagca cacttgatgc tgctaatatt ttaaaaccag ctttggcccg aggcacttta 1020

cgcactgttg gagcaacaac tcaagaggaa tatcaaaagc atattgaaaa agatgctgct 1080

ttatctcgtc gctttgcaaa agttttagta gaagaaccaa atcttgaaga cgcatatgaa 1140

attctacttg gattgaagcc agcgtatgaa gcctttcata atgttactat ttcagatgaa 1200

gcagttatga ctgctgtaaa agtggctcat cgttatttga caagtaagaa tttaccagac 1260

tcagctatag acttacttga tgaagcttct gccactgtgc aaatgatgat taagaaaaat 1320

gctccttcgc ttttaacaga agttgaccaa gctattttag atgatgatat gaaatctgct 1380

tctaaagctc taaaagcaag ttataagggt aaaaagcgta agcctattgc cgtaacagaa 1440

gatcatatta tggcaactct cagtcgtcta tctggtattc ctgttgaaaa attgacacaa 1500

gctgatagta aaaaatacct caatttagaa aaagaattgc ataaacgtgt tatcggtcaa 1560

gatgatgcag ttactgccat tagtcgtgct atccgtcgca atcaatctgg cattcgtact 1620

ggtaagcgtc ctattggttc ctttatgttc ttgggaccaa ctggtgttgg taagacagag 1680

ttagcaaaag ccttggcaga agtgttgttt gatgatgaat cagccctaat tcgttttgat 1740

atgtcggaat atatggaaaa attcgcagct tctcacctga atggggcacc tccaggttat 1800

gttggttatg atgaaggagg agagttgact gaaaaagtac gcaataaacc atattcagtt 1860

ttactttttg atgaagttga aaaagctcat ccagatattt ttaatgtatt attacaagtg 1920

cttgatgatg gcgttttaac agatagtcga ggacgaaaag ttgatttttc aaatactata 1980

atcattatga cttctaattt aggagcaact gcattacgtg acgataaaac agttggtttt 2040

ggagctaagg atatcagcca tgattatact gctatgcaaa aacgtattat ggaggagctt 2100

aaaaaagctt atcgaccaga gtttatcaat agtattgatg agaaagttgt attccatagt 2160

ctcagtcaag ataatatgcg tgaagttgtg aaaattatgg ttaaaccttt aattttagcg 2220

cttaaagata aaggtatgga tctgaaattc cagccttcag ccttgaaaca cttggcagag 2280

gatggctatg atatagaaat gggtgctcgt ccactgcgta gaactattca aacgcaagta 2340

gaggatcatt tatcagaact actattggca aatcaagtga aagaggggca agtaattaag 2400

attggcgtatccaaaggcaa attgaaattt gatattgcaa aatcataa 2448

SeqID 138

atggtcttag ataaagaaat caaggctcaa ttagcgcaat atttagattt gctagaatct 60

gatattgtat tacaagctga tttaggagat aacgataatt ctcaaaaagt aaaggatttt 120

ctagatgaaa ttgtcgctat gtctgatcgc atcagccttg aatcaacaca tttaaaacgt 180

caacctagtt ttggaattgc aaaaaaaggg catgaaagtc gcgttatttt ttcaggcttg 240

ccaatggggc atgaattcac atctttcatt ttagctttgt tgcaagtctc tggtcgcgca 300

ccaaaagttg atgaagatat tatcaaacga attaagggga ttgagaaaac tatcaatcta 360

gaaacttatg tttcattaac ttgccataat tgtccagatg tcgttcaagc ctttaatata 420

atggctgttt tgaatccgaa tatcactcat actatgattg aaggaggcat gtatcaagac 480

gaagtcaaat caaaaggtat catgtctgtt cctactgttt ataaggacca agaagaattt 540

acttctggcc gtgctacaat tgaacagctt ttagaacaac tagatggacc tcttgatgct 600

gaagcatttg ctgacaaagg tgtctatgat gttctagtta ttggtggtgg tcctgcaggt 660

aacagtgctg caatttatgc cgcccgtaaa gggttaaaaa caggtatttt agcagaaact 720

tttggtggac aagttatcga aacagttggt attgagaaca tgattggtac actgtacaca 780

gaaggcccta aattaatggc tcaaattgaa gagcatacca aatcttacga tattgatatt 840

atcaagagtc aattagcaac tggcattgag aaaaaagaat tagttgaggt aacactggct 900

aacggagcta ttttacaagc taaaactgct atcctagctc tgggtgctaa gtggcgaaat 960

atcaacgttc ctggcgagga agaattccgc aataaaggtg ttacttactg tccacattgt 1020

gatggccctc ttttcgaagg aaaagatgtt gctgttatag gtggtggtaa ttcaggtatg 1080

gaagctgctt tagatctagc cggtgtcact aaacatgtta cagtcctaga attcttacct 1140

gagctcaaag cagaccaagt actgcaagaa cgagcagcta aaactgacaa tttaactatt 1200

cttaagaatg ttgctactaa agatatcgtt ggtgaagatc atgtcactgg tttaaactat 1260

accgatcgtg atacaaacga agaaaaacac attgatttag aaggtgtttt tgtacaaatt 1320

ggtcttgtac caagtacatc ttggctcaag gatagcggca ttgaactcaa tgaacgtcaa 1380

gaaattgtcg ttgataaatt tggctcaaca aatatccctg gtatttttgc tgcaggtgac 1440

tgtacagatg ctgcctacaa gcaaatcatt atttccatgg ggtctggtgc cacagctgcc 1500

attggcgcat ttgattattt aatcagacaa taa 1533

SeqID 139

atgataaaat atcaagatga tttttaccaa gcggttaatg gggagtgggc taagacagct 60

gttattcctg atgacaaacc aaggacaggt ggtttttctg atcttgctga tgacattgaa 120

gcattaatgc tgtcaacgac agataagtgg ctggctgatg aaaataaacc aagtgatacg 180

attttaaatc attttattgc ttttcataaa atgacagctg attatcaaaa acgtgaagag 240

gtaggagtat caccagtatt accattaatt gaagaatata aaggcctaca atctttctct 300

gagtttgcat caaaagttgc agaatatgaa ctagagggta aaccaaatga atttccattt 360

ggtgtggctc ctgactttat gaatgctcaa ttgaatgtac tttgggctga agcgcctggg 420

attattttac cagatacaac ttattatagt gaagataatg aaaaaggaaa ggaactcctt 480

gctttttggc gtaaaagtca agaagaccta ctgcctttgt ttggcctttc agaacaggaa 540

attaaagata tcttggataa ggttttagca ttggatgcaa aattggcaca atatgtctta 600

tctcgcgaag agagttcgga atatgttaaa ctttaccatc cttataactg ggaagatttc 660

acaaaattag cacctgaact tccattagat gctattttcc aaaagatatt aggacaaaaa 720

ccagataaag ttattgttcc tgaagaacgt ttctggacag aatttgcatc tgactattac 780

tcagaatcta attgggaatt attaaaggca gatcttattt tatcagctgc gaatgcctat 840

aatgcttact taactgatga tattcgtatc aaatcgggag tttacagtcg agctttatca 900

ggcacaccac aagcaatgga taagaaaaaa gcagcttact accttgcttc aggaccttac 960

aatcaagctc taggtttgtg gtatgcagga gaaaaattct ctccagaagc taaagctgat 1020

gttgagcata agattgcaac gatgattgat gtctataagt cacgcttaga aaaggcggat 1080

tggcttgcac agtcaacgcg agaaaaagct attatgaaac ttaatgttat cacaccacat 1140

attggatacc ctgagaaact accagagact tatactaaaa aaatcattga tcctaaacta 1200

tcgcttgtcg aaaatgctac taatttagac aaaatttcaa tagcctatgg ttggagtaaa 1260

tggaataaac cagttgatag aagtgaatgg catatgcctg cccacatggt caatgcgtat 1320

tatgatccac aacaaaacca aattgtcttc ccagcagcca ttttacaaga accattttat 1380

gctttagagc aaagttcatc agcaaattat gggggaattg gtgcagtgat tgcacatgaa 1440

atatcacacg cttttgacac taatggagct tcatttgatg aacatggaag tttgaataat 1500

tggtggactg atgaagattt tgaagctttc aaaaaactta cagataaagt agttgagcag 1560

tttgatggac tggaatctta tggcgcaaaa gtcaatggta aactaacagt atcagaaaat 1620

gttgcagatt taggtggagt tgcttgtgct ttagaagctg cgcagcgtga gtcagatttt 1680

tcagcgaggg atttctttat taactttgcg acaatttggc gtatgaaggc acgtgatgag 1740

tatatgcaaa tgttagctag tgtagatgtt cacgccccag cacaatggcg aactaatatt 1800

acagtaacga attttgaaga attccataaa gagtttgatg ttaaagatgg tgataacatg 1860

tggcgtccag ttgaaaaacg tgttattatt tggtaa 1896

SeqID 140

atgtctaaga ttcttttttt cggtgaacct ctcattcgca tcactccaaa agaaaatgac 60

tattttgcag atagtatctc aactaaacta ttctatggtg gctctgaagt taataccgca 120

agagctctac aaggctttgg gcaagatact aaactcctct cagccttacc caataatccc 180

ataggtaata gttttcttca atttttaaaa gcacagggta ttgatactca ttctattcag 240

tgggttggag aacgtgttgg gttatatttt ttagaagatt cctttgcttg cagaaaaggt 300

gaggtggtct acgaccgcga tcatagtagt ttacatgact ttaggataaa tcaaattgat 360

ttcgatcaat tatttgaagg agtttctctc tttcatttta gtggtattac actatcattg 420

gatgagtcta tccaagagat aacactgctt cttttaaaag aagctaaaaa aagagagata 480

acaatctcct tagatctcaa ttttcgcagt aaactaattt ctcctaaaaa tgccaaaatc 540

cttttttctc agttcgctac ctttgcggat atttgttttg gtattgaacc tcttatggtt 600

gactcacagg acactacctt cttcaatcgc gatgaagcta caatagagga cgtcaaagaa 660

cgaatgattt cgcttataaa tcactttgac tttcaagtca ttttccatac taaacgtcta 720

caggatgaat ggggacgaaa ccactaccaa gcttacatag caaatcgtaa acaagagttt 780

gtcacttcaa aagaaatcac cacagctgta aaccagagaa ttggaagcgg agatgctttt 840

gtggcgggag cgctatatca actacttcag cactcagaca gcaagacagt cattgatttc 900

gctgtcgcta gtgcatcttt aaaatgtgct ttggaagggg acaatatgtt tgagaccgtt 960

accgcagtta acaaagtact aaatcaatcg aaagacatta ttcgttag 1008

SeqID 141

atgaaaagtc gaaaaaaaga taaattggta ttgaggttaa caacaacact attggttttt 60

ggtttgggtg gggtttggtt ttataattat aaaaatgata atgtcgaacc gacagtcact 120

agtgcatcgg atcaaacgac gacttttatt caaacgattt ctccaacagc tattgaaatt 180

tctaagacct atgatttgta tgcgtcagtc ttattagcac aagctatttt ggaatcatcc 240

agtggacaat cagatttgtc taaggctcct aattataacc tctttggcat caaaggagaa 300

tataaaggta aatctgtcca aatgcctact ttagaagatg atgggaaagg caatatgact 360

caaatccaag ctccttttcg cgcctatcca aattattctg cttcactata tgattatgct 420

gagttagtat ctagtcaaaa gtatgcatct gtttggaaat caaatacctc ttcttataag 480

gatgctactg cagctctaac aggtctttat gcgacagata ctgcttatgc tagtaaatta 540

aaccaaatta ttgaaaccta cagtctagat gcttatgata aatag 585

SeqID 142

atggcagacg tgaaggtagt gaataatgag gattctagag gtcaaaagca agacttaaag 60

gctaaactat ttcatattaa gatagggtca gttcccctac cagtatatgt ttgtttagca 120

ttattgattc ttctagcagg ctttttacaa aaattgccag tcaatatgct aggaggattt 180

gcagttatct taacaatggg gtggttctta gggactatcg gagctagcat tcctggtttt 240

aaaaacttcg gtggcccagc tattttatct ttattagtac catctatttt ggtgtttttc 300

aacctcatta ataaaaatgt tttagaatca acaaatatgt tgatgaagca agctaacttt 360

ctttattttt atattgcttg tttagtgtcc ggtagtattt tagggatgaa tcggaaaatg 420

ttgattcagg gattgctaag aatgattttc cccatgttat taggaatggt ttgtgcgatg 480

atggtaggga catttgtcgg tgttatttta ggcttagagt ggcgacacac tttgttttat 540

atcgtaacac ccgttttagc tggtggtatt ggtgaaggta ttttaccat tatcgttaggc 600

tatagttcaa ttaccggtgt agctagtgaa caactagttg ctcaactcat cccagccact 660

attattggta atttctttgc cattttatgt actgcactat tgaatcgttt gggagaaaag 720

aaaccacact tgtctggtca agggcaatta gtaaggttaa ataaaggaga ggacatgtca 780

gatattattg ctgatcattc tggcccaatt gacgttaaga aaatgggtgg aggtgtttta 840

acagcatgta gtctctttat ttttggacat ttgttgcagc aattaactgg atttcctggt 900

cccgtattaa tgattgttgc agcagctatt ttgaaatata ttaatgttat tcctagagaa 960

acacaaaatg gagctaagca actttataaa tttatttctg gtaattttac atttcctcta 1020

atggcagggc taggattgct ttatatcccg ttaaaagatg ttgtggcaac gcttagcata 1080

caatatttca tagttgttat tagtgttgta tttacagtta tttctgttgg attctttgta 1140

tcgcgattcc ttaatatgaa tcctgttgaa gcaggtatta tttcagcttg tcaaagtggt 1200

atgggaggaa caggagatgt tgccatttta agtacagcag accgaatgaa cttgatgcca 1260

tttgctcaag ttgctacgcg tttaggagga gctattactg ttatcacaat gacagccatt 1320

ttacgcatgt tattctaa 1338

SeqID 143

agaggcaaaa aagctggtaa atacacaaca tctgatggtt acatttttga tgccaaagat 60

attaaaaaag atacaggtac aggttatgtc attccacata tgacacatga gcattgggta 120

ccaaagaaag atttatcaga gtcggaatta aaagcagctc aagaatttct ttcaggaaaa 180

tctgaagcaa atcaagacaa accaaaaaca ggtaaaacag ctcaagaaat ctatgaggca 240

attgaaccaa aagcaattgt taaacctgaa gatttattat ttggaattgc acaagcgaca 300

gactataaga atggtacatt tgtaattcct cataaagatc attaccatta tgtggaatta 360

aaatggtttg atgaagaaaa agatctttta gctgattcag ataagacata ttctttagaa 420

gactatttag ctacggctaa atattacatg atgcacccag aaaaacgtcc taaagttgaa 480

ggatggggta aagatgctga aatttataag gaaaaggact ctaataaagc agataaacca 540

agtcctgcac caactgataa taaatcaaca tcaaattcta gtgacaaaaa cttaagtgct 600

gcagaagtat tcaaacaagc aaaaccagaa aaaattgtac cgcttgataa aattgctgct 660

cacatggcat atgcagttgg atttgaagat gatcaattga ttgttcctca tcatgatcat 720

tatcataatg ttcctatggc atggtttgac aagggtggtt tatggaaagc accagaaggc 780

tatacattac aacaactctt ctcaacaatt aaatactaca tggaacatcc taatgaatta 840

ccaaaagaaa aaggttgggg acacgacagt gatcataaca aaggctcaaa taaagacaat 900

aaagccaaaa attatgctcc agatgaagaa cctgaagatt cagggaaagt aactcacaac 960

tatggttttt atgatgttaa taaaggttca gacgaagaag aaccagaaaa acaagaagat 1020

gaatcagagc tagatgaata tgaactagga atggcacaaa atgctaagaa atatggtatg 1080

gatagacaat cttttgaaaa gcaactcatc caattatcaa ataaatatag tgtaagtttt 1140

gaaagcttta attatattaa tggtagtcaa gttcaagtta ctaaaaaaga tggctctaaa 1200

gtgcttgttg atattaaaac actaacagaa gtgaaataa 1239

SeqID 144

atgaatcgta aaaaaacagt tattattagt gctttatcag tagctctatt tggtactggt 60

gtaggtgctt accaattagg tagctataat gcccaaaaat cagacaatag tgtttcttat 120

gtgaaaacag ataaatctga tagcaaagca caagcaacag ctgtaaataa aacgcccgac 180

caaatcagta aagaagaagg catatctgca gaacaaatcg ttgttaaaat tactgacgac 240

ggttatgtga cttctcatgg agaccattac cattattaca atgggaaagt gccatatgat 300

gccatcatta gtgaagaatt gattatgaaa gaccctagct atgtctttaa taaagctgat 360

gtcattaatg aagtcaaaga tggttatatt atcaaagtta acggtaagta ttacctctat 420

cttaaagaag gtagcaaacg aacaaatgtt cgtacaaagg aacaaatcca aaagcaacgc 480

gaagaatggt ctaagggtgg ttctaaggga gaatcaggaa agcatagtag tgcaaaaact 540

caagctttat cagccagcgt acgtgaagcg aaagctagcg gtcgctatac tacagatgat 600

ggttatgttt ttagtccaac agatgtcatt gatgacatgg gagatgcctt tttagtacca 660

catggcgatc atttccatta tatcccgaaa gctgatttat ctccatcaga gttatcagcc 720

gcacaggcat attggaaccg caagactggc cgatcaggta atagctcaaa accatcaaat 780

tcaagttcat acattcatgc aagtgcacca agcggtaatg tatcaacagg aagacatgct 840

aatgccccta tttctattcc aagagtcact cacgcaaatc actggtcaaa accagctggt 900

aatcatgcta cggcaccaaa acaccatgca cctacaacca aaccaatcaa caaagattct 960

gctttagaca aaatgttgaa acgtttatat gctcaaccgt tatatgctcg tcacgtagaa 1020

agtgatggtt tggtttatga cccagcacaa gttaatgctt tcacagcaat cggtgtttct 1080

attccgcacg gtaatcattt ccactttatt cactataagg atatgtctcc attagagtta 1140

gaagcaacaa ggatggtggc agagcataga ggacatcata ttgatgcatt agggaaaaaa 1200

gattctacag agaaaccaaa gcatatttct catgaaccta ataaggaacc tcacacagag 1260

gaagaacacc atgcagtaac accgaaagac caacgtaaag gcaaaccaaa tagccagatt 1320

gtctacagtg ctcaagaaat tgaagaggca aaaaagctgg taaatacaca acatctgatg 1380

gttacatttt tgatgccaaa gatattaaaa aagatacagg tacaggttat gtcattccac 1440

atatga 1446

SeqID 145

atggctaaat ttggattttt atcggtacta gaagaagaat tggataagca tttacagtat 60

gactttgcca tggattggga taagaaaaat catactgtag aagtcacttt tatattagaa 120

gcacaaaaca gctctgctat tgaaaccgtg gacgaccagg gggagacgag tagcgaggac 180

atcgtttttg aggactatgt actcttttac aaccctgtca agtctcgctt tgatgctgag 240

gactatctgg tgactatccc ctatgagcct aagaagggct tgtcgcgtga gtttctggct 300

tattttgcgg agactctcaa cgaggttgcc actgaagggt tgagcgacct catggacttc 360

ctcaccgatg acagtatcga agagttcggt ctttcttggg atacggatgc ttttgaaaat 420

ggcagagcag aactgaaaga gacagagttt tacccttatc cgagatatta g 471

SeqID 146

atgaacacaa aacagcgttt ttcaatccgg aaatataagt taggtgccgt atctgtactt 60

ttgggaaccc tatttttttt aggtggtatc acaaatgtag ctgctgattc tgtcataaat 120

aagccatctg atattgcagt tgaacagcaa gtaaaagaca gtccaacgag catagcaaat 180

gagacaccta ctaacaacac gtcatcagcc cttgcgtcga cagctcaaga caatcttgtt 240

acaaaggcta ataatagtcc aacagaaaca caaccagtag ctgagtctca ctctcaagcc 300

accgagacat tttccccagt cgcaaatcaa ccggttgaaa gcactcaaga agtttctaaa 360

actcctttaa ccaaacaaaa tttagcagtc aaatctacac cagctatttc taaagaaacc 420

cctcaaaaca ttgatagtaa taaaattatc actgtcccca aagtatggaa cacaggctac 480

aaaggagagg gaactgttgt agcaattatt gactcaggac tagatatcaa tcacgatgct 540

ctccaattaa atgattcgac aaaagcaaaa taccaaaacg aacagcaaat gaatgctgct 600

aaagcaaaag ctggtataaa ctatggaaaa tggtataaca acaaagtaat ctttggtcac 660

aactatgttg atgtcaatac agagctaaaa gaggtgaaaa gcacttctca tggtatgcac 720

gtaaccagta tcgcaacagc taatcctagc aagaaagata caaatgaatt aatctatggt 780

gttgctcctg aagcacaagt aatgtttatg agagtcttct ctgatgaaaa aagaggaact 840

ggaccagccc tttatgttaa agctattgaa gatgccgtta aactcggtgc tgacagcatt 900

aatttaagtt taggtggagc taatgggtct ttagttaatg ccgatgaccg acttataaaa 960

gctttagaga tggctagact cgctggcgtt tctgttgtta tagcagcagg taacgacggt 1020

acatttggga gtggagcatc aaagccttct gctctttatc ctgattatgg tttagttggt 1080

agtccatcaa cagctcgtga ggccatttct gtagcatcat ataataatac aacactggtt 1140

aataaagtct tcaacattat cggattagaa aacaacagaa atctcaacaa cggattagct 1200

gcttatgcag atcctaaagt tagtgataag acctttgaag tagggaaaca atatgattat 1260

gttttcgtag gaaaaggaaa cgacaatgat tataaggaca aaactttaaa tggtaaaatc 1320

gccttaattg aacgtggaga tattactttt acaaaaaaag tcgtcaatgc tattaatcac 1380

ggtgctgtgg gagctattat ctttaataac aaagctgggg aagctaatct aacaatgagt 1440

ttagatcctg aagcaagtgc tattcctgct atttttaccc aaaaagagtt tggagatgtt 1500

ttagctaaaa acaactataa aattgtattt aacaatatca aaaataaaca agccaaccct 1560

aatgcaggtg tcctatctga cttttcaagc tgggggttaa cagcagacgg acaattaaaa 1620

cctgacttat ctgctcctgg aggctctatt tacgccgcta tcaatgataa tgaatatgat 1680

atgatgagtg ggacaagtat ggcttctccc catgtcgctg gtgctactgc tctagttaaa 1740

caatacttat tgaaagaaca tccagaactt aaaaaaggtg acattgaaag aactgtcaaa 1800

taccttctta tgagtactgc taaagcacac ctaaacaaag atacaggcgc ttacacctca 1860

ccacgccaac aaggagcagg tattatcgat gtcgcagcag cagttcagac aggattatac 1920

ctaactggtg gggaaaacaa ctatggcagc gttacattag gaaatattaa agataaaatt 1980

tcctttgatg ttactgttca taatatcaat aaagttgcaa aagatttaca ctatacaacc 2040

tatttaaata ctgatcaagt taaagatggc tttgtcacat tggctcctca acaacttggt 2100

acatttacag ggaaaacgat acggattgaa ccagggcaaa cccaaacgat tacaattgat 2160

atagatgttt cgaaatacca tgacatgtta aaaaaagtaa tgccaaacgg ctatttccta 2220

gaaggctacg tacgttttac agaccctgtt gatggtgggg aagttcttag tattccttat 2280

gttggattta agggagaatt ccaaaactta gaagttttag aaaaatccat ttataagctt 2340

gttgctaaca aagaaaaggg attttatttc caaccaaaac aaacaaacga agttcctggt 2400

tcagaagatt atactgcctt aatgactaca agttcagagc ctatctactc aacagacggt 2460

actagtccta tccaattgaa agccttggga agctataagt ctatagatgg aaaatggatc 2520

ttacaactag atcaaaaagg ccagcctcat ctagccattt cacctaatga tgaccaaaat 2580

caagatgccg ttgcagtgaa aggtgttttc ttacgtaatt tcaataattt aagagccaaa 2640

gtctatcgtg cagatgatgt taatttacaa aaaccactat gggtaagtgc tccccaagca 2700

ggagataaaa attactacag cggaaatact gaaaatccaa aatctacatt tttatatgac 2760

acagaatgga aaggaaccac tactgatggt attcctttag aagatggaaa atacaaatac 2820

gttttaactt attactctga tgtccctggc tctaagccac aacaaatggt gtttgatatc 2880

actttggata gacaagctcc tacactaaca acagcaactt atgacaaaga tagacgtatc 2940

ttcaaagctc gtcctgcagt agaacacggg gaatctggta tctttagaga acaagtttgt 3000

tacttaaaaa aagataaaga tggtcattat aatagcgtct tacgtcaaca aggagaagac 3060

ggtatccttg ttgaagataa caaagtattt atcaaacaag aaaagaatgg tagctttatt 3120

ctacctaaag aggttaacga tttctctcat gtctactata ctgttgaaga ttatgcaggc 3180

aatctagtat cagcaaaact cgaagatttg atcaatattg gcaataaaaa tggtttagta 3240

aacgtcaaag tgtttagccc tgagcttaac agtaatgtcg atattgattt ctcttactct 3300

gtcaaagatg acaaaggtaa tatcatcaaa aagcaacatc acgggaaaga cctcaattta 3360

ttgaaattgc cttttggtac ctatacgttt gatctattct tatacgatga ggaacgagca 3420

aatctaatca gtcccaaaag tgtcactgta actatttctg aaaaagatag ccttaaagac 3480

gtcttattta aagttaactt actcaagaaa gcagccttac tcgttgaatt tgacaagctt 3540

ttaccaaaag gagcaacagt ccagttggtt actaagacaa atactgttgt tgatctacca 3600

aaagcaactt attctcctac tgactatggt aaaaacatac ctgtaggaga ctatcgttta 3660

aacgtaacgc tgcctagtgg gtatagcact ttagagaact tagatgattt acttgtatcc 3720

gtaaaagaag atcaggtaaa cctaacaaaa ttgacgctga ttaataaagc tcctatgatt 3780

aatgccctag cagaacaaac tgatattatt acccagcctg tgttttataa tgctggaact 3840

cacttaaaaa ataattacct agctaatctt gaaaaggcac aaactttaat taaaaataga 3900

gtggaacaaa caagtattga taatgctatt gctgctttga gagaaagtcg ccaagctctt 3960

aacggtaaag aaacagatac ttctttactg gcaaaagcta ttttagctga aacagaaatc 4020

aagggaaact atcaatttgt taatgctagt ccattaagcc aatcaactta tatcaatcaa 4080

gtccaattgg cgaaaaacct tctacaaaaa cctaacgtca ctcaatcaga agtagacaaa 4140

gccttagaaa atcttgatat tgctaaaaat caattaaatg gtcatgaaac tgattactct 4200

ggtttacacc atatgataat taaagcaaac gttctgaaac aaacatcatc taaatatcag 4260

aacgccagtc aatttgataa agaaaattat aataacctta tcaagaaagc agaattgctg 4320

ctttccaata gacaagctac acaagctcaa gttgaagagt tattaaacca aataaaagca 4380

accgaacaag agcttgatgg ccgtgataga gtttcttccg cagagaatta tagtcaatca 4440

ctcaatgata atgactctct caatacaaca cctatcaatc cgccaaatca gccccaggcg 4500

ttgatattca aaaaaggcat gactaaagaa agtgaggttg ctcagaagcg tgtcttaggg 4560

gtgactagcc aaaccgataa tcaaaaggta aagacaaaca agcttcctaa aacaggcgaa 4620

agcactccta aaataaccta tacaatattg ctatttagtc tctctatgct aggtctggca 4680

acaatcaaac taaagtctat caaaagagaa taa 4713

SeqID 147

atgaataata acgaaaaaaa agtaaaatac tttttaagaa aaacagctta tggtttggcc 60

tcaatgtcag cagcgtttgc tgtatgtagt ggtattgtac acgcggatac tagttcagga 120

atatcggctt caattcctca taagaaacaa gttaatttag gggcggttac tctgaagaat 180

ttgatttcta aatatcgtgg taatgacaaa gctattgcta tacttttaag tagagtaaat 240

gattttaata gagcatcaca ggatacactt ccacaattaa ttaatagtac tgaagcagaa 300

attagaaata ttttatatca aggacaaatt ggtaagcaaa ataaaccaag tgtaactaca 360

catgctaaag ttagtgatca agaactaggt aagcagtcaa gacgttctca agatatcatt 420

aagtcattag gtttcctttc atcagaccaa aaagatattt tagttaaatc tattagctct 480

tcaaaagatt cgcaacttat tcttaaattt gtaactcaag ccacgcaact gaataatgct 540

gaatcaacaa aagctaagca aatggctcaa aatgacgtgg ccttaataaa aaatataagc 600

cccgaagtct tagaagaata taaagaaaaa attcaaagag ctagcactaa gagtcaagtt 660

gatgagtttg tagcagaagc taaaaaagtt gttaattcca ataaagaaac gttggtaaat 720

caggccaatg gtaaaaagca agaaattgct aagttagaaa atttatctaa cgatgaaatg 780

ttgagatata atactgcaat tgataatgta gtgaaacagt ataatgaagg taagctcaat 840

attactgctg caatgaatgc tttaaatagt attaagcaag cagcacagga agttgcccag 900

aaaaacttac aaaagcagta tgctaaaaaa attgaaagaa taagttcaaa aggattagcg 960

ttatctaaaa aggctaaaga aatttatgaa aagcataaaa gtattttgcc tacacctgga 1020

tattatgcag actctgtggg aacttatttg aataggttta gagataaaca aactttcgga 1080

aataggagtg tttggactgg tcaaagtgga cttgatgaag caaaaaaaat gcttgatgaa 1140

gtcaaaaagc ttttaaaaga acttcaagac cttaccagag gtactaaaga agataaaaaa 1200

ccagacgtta agccagaagc caaaccagag gccaaaccaa atattcaagt acctaaacaa 1260

gcacctacag aagctgcaaa accagctttg tcaccagaag ccttgacaag attgactaca 1320

tggtataatc aagctaaaga tctgcttaaa gatgatcaag taaaggacaa atacgtagat 1380

atacttgcag ttcaaaaagc tgttgaccaa gcttatgatc atgtggaaga gggaaaattt 1440

attaccactg atcaagcaaa tcaattagct aacaagctac gtgatgcttt acaaagttta 1500

gaattaaaag ataaaaaagt agccaaacca gaagccaaac cagaggccaa accagaagct 1560

aagccagaag ctaagccaga agctaagcca gaagctaagc cagaggccaa accagaagct 1620

aagccagacg ttaagccaga agctaaacca gacgttaaac cagaggctaa gccagaagct 1680

aaaccagagg ctaagtcaga agctaaacca gaggctaagc tagaagctaa accagaggcc 1740

aaaccagcaa ccaaaaaatc ggttaatact agcggaaact tggcggctaa aaaagctatt 1800

gaaaacaaaa agtatagtaa aaaattacca tcaacgggtg aagccgcaag tccactctta 1860

gcaattgtat cactaattgt tatgttaagt gcaggtctta ttacgatagt tttaaagcat 1920

aaaaaaaatt aa 1932

SeqID 148

atggcaaaag atattaaatt ttcagcagat gcccgctcag caatggtgcg tggtgttgat 60

attttagctg atacagtcaa agtaacatta ggtcctaaag gccgtaatgt tgttcttgaa 120

aaagcatttg gttctccttt aattacaaat gatggtgtga caattgctaa agaaattgag 180

ctagaagatc actttgaaaa tatgggagct aaacttgtgt cagaagtggc ttcaaaaact 240

aatgatattg caggggatgg cactacaact gctactgttt tgacccaagc tattgtacgg 300

gaaggtctta aaaatgtaac tgcaggggca aatccgattg gcattcgtcg tggtattgaa 360

acagctgttt cagcagcagt tgaagagcta aaagagattg cacaaccagt ttcaggcaaa 420

gaagctattg ctcaagttgc ggctgtgtct tcacgttctg aaaaagttgg ggaatacatt 480

tctgaagcta tggggcgcgt gggtaatgat ggtgttatca ctattgaaga atcgcgaggt 540

atggaaacag agcttgaagt tgtggaagga atgcagtttg accgtgggta cttgtcacag 600

tatatggtaa ctgataacga gaaaatggtc tctgaacttg agaatccgta tatccttatt 660

acagataaga aaatttcaaa tatccaagaa attttaccat tattagaaga ggttcttaaa 720

acaaatcgtc cgttgctaat catcgctgat gatgttgatg gagaagctct cccaacgctt 780

gttcttaaca aaattcgtgg aactttcaat gtcgtagctg ttaaagcgcc tggatttggt 840

gatcgtcgta aagccatgct ggaagatatt gctatcctaa caggaggaac tgtcgttact 900

gaagaccttg gtttagactt aaaagatgct actatgcaag ttttaggaca gtctgctaaa 960

gtaacagtag ataaagattc tactgttatt gtcgaaggtg ccggtgactc atcagcaatt 1020

gctaatcgcg tagctatcat taagtcacag atggaggcta caacttctga ttttgatcgt 1080

gaaaaattac aagaacgact tgctaagtta gccggtggtg tagcagtaat taaagttggt 1140

gcagcgactg aaacagaatt aaaagagatg aaacttcgca tcgaagatgc gttaaatgca 1200

acgcgtgctg cagttgaaga aggtattgtt tcaggtggag gtacggctct tgtgaacgtt 1260

attgaaaaag tagcggcact gaaacttaat ggtgatgagg agactggacg taatattgtt 1320

cttcgtgcta tagaagagcc tgttcgtcaa attgcttaca atgctggata tgaaggttca 1380

gttattattg aacgtttaaa acagtctgaa attggtacag gatttaatgc ggccaatgga 1440

gaatgggtag atatggttac cacaggtatc attgaccctg tcaaagtaac acgttctgca 1500

cttcaaaatg cggcatctgt agcaagtctt atcttgacta cagaagcagt agtagcaaat 1560

aaacctgaac cagaagctcc tacagctcct gcaatggatc catctatgat gggtggcttc 1620

taa 1623

SeqID 149

gtggtagaaa acctagaaaa accaattgga gtgagctata aaaatagccc atcaatgagt 60

aaacgaacgg ctatcagaat gaagaaatct agtcgatttt caattttact atatagtgtt 120

ttgtcaactc tcttagcaat tgcaaatcct ttattgactt attttgcaaa tggtttgcag 180

actcagaatc tctatacagg tttgatgatg accaaaggac agatccctta tagtgatgtt 240

tttgctacag gaggattttt atactatgta acgattgctc taagttacct tttagggtct 300

agtatctggt tacttattgt acagtttatt gcttactatg tatctggaat ttatttttat 360

aaattagttt attatgtggc acaaagtgaa attgtctcga taggcatgac gttgattttc 420

tatataatga atattgtctt aggattcggt ggtatgtacc caatacagtg ggcattacct 480

tttatgctca tttcgctatg gtttttaatt aaattttgtg tcgataatat cgttgatgaa 540

gcatttatat tttatggcat tttagcagca ttctcactat ttatagatcc ccaaacactg 600

attttttggc tttgctcttt tgttctctta acagcaacca atattaagca aaagcagtca 660

ctacgaggat tttatcagtt tttatgcgtt gtttttggta tgattttaat tgcttatact 720

gttggttact ttatgtttaa tctacagatt atctcttctt atattgacaa agctattttt 780

taccccttta cgtatttcgc taggacaaac cactcattcc ttcttagttt agcaattcaa 840

attgttgtcc ttctagggtc aggttgtctc tttggactat gggattttat tcaaaatcgt 900

aaaaaagcat cttatcaaat cggcttgaac tttattgcct gtatttttat tatttacgct 960

ataatggcca ttttttcaag agattttaac ctttaccact ttttacctgc tttaccattt 1020

ggtcttttgc taacgagtaa taaaattact attctttacc aaaaagtgat tgacagacgt 1080

tcacatagac gtcagtattt ctctggtaaa tctcttatcg tagacctttt tgtaaagaaa 1140

acatattatt tgcccctctt actagtttcc ttatcgatag ggttactagt ttataatact 1200

tatcaaaatg ttactcttag taaagaaagg cgtgacattt cacattattt gactacaaaa 1260

attgatagag atggtaagat ttatgtttgg gacaaagttg ctagcattta tagccagact 1320

cggttaaaat ctgcttctca atttgttttg ccacatatta atacagcgca aaaaaataat 1380

gagaaaattt tgaaggacga gctgcttcag catggggcta aatactttat cttaaataaa 1440

aatgagaagt taccaaatga actgaaatct gatattaaga agcactacca agaagttcct 1500

ctatcaaata tcactcattt tgttctatat cgtttcaaat ag 1542

SeqID 150

atggccaagc caacgatatc accgggaatg caacagtatc tggatataaa agagaattat 60

ccagatgctt ttttgctttt tagaatgggt gatttttatg aattatttta tgatgatgcg 120

gtaaaagcag cacaaatcct ggaaattagc ttgactagtc gaaataagaa cgcagaaaag 180

ccaatcccaa tggcaggagt tccctatcac tcagctcaac agtatattga cgttttagtt 240

gaattaggtt acaaagtagc cattgctgag cagatggaag atcctaaaaa agctgtggga 300

gtggtcaagc gtgaggtagt gcaagttgtt accccaggaa cggttgtgga gtcaacgaag 360

ccggatagtg ctaataattt cttagtagcg attgattcgc aagatcaaca aacatttggt 420

ctagcatata tggatgtctc aactggagag tttcaggcaa cccttttaac agattttgag 480

tccgtccgta gtgaaatact aaatttaaaa gctcgtgaga ttgtagtagg atatcaattg 540

acggacgaaa aaaatcacct actgacgaag cagatgaact tgcttttatc atacgaagac 600

gaacgactta atgatattca tttgattgat gagcagttaa ctgatttgga aatatctgct 660

gcggaaaaac ttttacaata tgtgcataga acacaaaagc gtgaacttag tcatttacag 720

aaagtagttc attatgaaat aaaggactat ttacaaatgt catatgcaac gaaaaatagt 780

ctagatttac tggagaatgc tagaacaagc aagaagcatg gaagtcttta ctggttgtta 840

gatgagacta aaacagcgat gggaactcga atgctgagaa cttggattga caggcctttg 900

gtaagtatga atcgaatcaa ggaacgtcaa gatattattc aagtgtttct tgattatttt 960

tttgagagaa acgatctcac agaaagttta aagggtgtat atgatattga acgcctagca 1020

agtcgagtat ctttcggaaa agccaaccct aaagatctat tgcaactcgg acagacctta 1080

tcacaaattc ctcggattaa aatgatttta cagtccttca atcaacctga gcttgacatc 1140

attgtcaaca aaattgacac tatgcctgaa ttagaaagtt taattaatac ggcgatagcc 1200

ccagaagcac aggctactat cactgaggga aacattatca agtctggatt tgataagcaa 1260

ttggataatt atcgaacagt gatgcgtgaa ggtacaggtt ggattgctga tattgaagct 1320

aaggaaagag cagcaagtgg tatcggtact cttaaaattg attataataa aaaagacggt 1380

tattacttcc atgttaccaa ttccaattta tcactagtac cggagcattt tttccgtaaa 1440

gcgacattaa aaaattctga acgctatgga acagcagaac tagccaaaat tgaaggtgaa 1500

atgctcgaag ctcgcgagca atcttcaaat ttagaatatg atatttttat gcgtgttcgt 1560

gcccaagtag aatcttatat taaacgtctt caagagttag caaagacgat tgcaaccgtt 1620

gatgttctac agagtttggc agtagttgca gaaaattatc actatgttcg tcccaaattt 1680

aatgatcaac atcagattaa gattaagaat gggcgtcatg caactgttga aaaagtgatg 1740

ggagtgcaag aatatattca caatagcatc tattttgata gtcagacaga tatccagttg 1800

attacaggac caaatatgag tggtaagtcg acctatatgc gccagttagc tttgacagtt 1860

attatggcac aaatgggagg ttttgtatcg gcagacgaag ttgatttgcc tgtatttgat 1920

gcaatattta ctaggattgg tgatgctgac gacttaattt ctgggcaatc aacctttatg 1980

gtagaaatga tggaagcgaa tcaagctgta aaacgagcca gtgataaatc tttgattctt 2040

tttgatgaat taggtcgagg gacagccact tatgatggta tggcattagc tcaatcgatt 2100

atagaatata ttcatgaccg tgttagggca aaaacaatgt ttgcgactca ttaccatgag 2160

ttgacagatt tatctgaaca gttgacaagg cttgtcaatg tacacgtggc tactttagag 2220

agagatggag aagttacctt cttacataaa attgaatctg gacctgcgga taagtcttat 2280

gggatacacg tcgcaaaaat agctggttta ccaattgact tattggatag ggcaactgat 2340

attttatcac agttggaagc tgatgcagta cagttgatcg tatcgccctc ccaagaagct 2400

gttactgctg acttaaatga ggaactagat tctgagaagc aacaaggaca attatcgctt 2460

tttgaagaac cttcaaatgc aggtagggtt attgaggagt tagaagcgat agatataatg 2520

aatctaactc caatgcaagc tatgaatgct atatttgact taaagaaatt attataa 2577

SeqID 151

atgttgaaac tggatttaaa aacaaagata aaagaagcta ttttaattgc ctttggtgtg 60

gctctctata ctttcggctt tgtcaaattt aatatggcta atcatttggc tgaaggaggt 120

atttccggtg tcactttgat tatccatgca cttttcggag ttaatccagc actttcatca 180

cttctcctta atatcccgtt atttatatta ggggctagga ttttagggaa aaaatcctta 240

ttattaacta tatatggtac agtgttaatg tcttttttca tgtggttttg gcaacaaatc 300

cctgtaacag tccctttgaa aaacgatatg atgttagttg cagtagctgc tggaatttta 360

gcaggaactg gtagtggttt agtatttaga tatggtgcaa ctacaggtgg tgcagatatt 420

attggtcgta ttgtagagga aaaatctgga ataaaattag ggcaaacctt attgtttatt 480

gatgctatag ttctaacgtc ttcacttgtt tatattaatt tacaacagat gctatatacg 540

ttagtagcta gctttgtatt tagtcaagtg ttaacaaatg ttgaaaatgg gggctacact 600

gttcgtggaa tgatcattat taccaaagaa tcagaatctg cagcagcaac cattttgcat 660

gaaattaata ggggtgtaac ttttttacgg ggccaaggtg cctactcagg tagagagcat 720

gatgtcttgt acgtagctct taacccaagt gaagtgcgtg atgtgaaaga aatcatggct 780

gatttagacc cagatgcttt tatttctgtt ataaatgtag atgaagttat tagctcagat 840

tttaaaattc gacgaagaaa ttacgataaa taa 873

SeqID 152

atgaaacgta gtatgtatgc tgggcgtgtt cgtagtgagc acataggaac atcgattact 60

ttaaaaggat gggttggtcg tcgtcgtgac cttggaggcc ttatttttat tgatcttcgc 120

gatcgcgagg gaatcatgca attagttatt aatcctgaag aagtagcagc ctcagttatg 180

gcaaccgcag aaagtctccg tagtgaattt gttatagagg ttagcggtgt agtgacagca 240

cgagaacaag ctaatgataa tttaccaaca ggagaagttg aattaaaagt gcaagaatta 300

tcggttctta atacctcgaa aacaacacca tttgaaatta aagatggtat tgaggcaaat 360

gatgatacac gcatgcgtta ccgttacttg gatcttcgtc gtccggaaat gcttgaaaat 420

ttcaaattac gtgcgaaagt aacacactca atccgtaatt accttgacaa cttagagttt 480

attgatgtcg aaactccgat gctaacaaaa tcaactccag agggggcacg agactatttg 540

gttccatcac gtgtcaacca aggacatttt tatgcccttc cacaaagccc tcaaattaca 600

aaacagctat tgatgaatgc aggatttgat cgttattatc aaattgtcaa atgttttcgt 660

gatgaggatt tacgtggaga tcgtcaacct gagtttacac aagttgactt agagacatca 720

ttccttagtg atcaagaaat tcaagacatt gttgagggta tgattgctaa agtaatgaaa 780

gacactaaag gtttagaggt ttctttacca ttcccaagga tggcatatga tgatgcaatg 840

aataactacg ggtctgataa gcctgataca aggtttgata tgcttttaca agacttgaca 900

gaaattgtaa aagaagttga cttcaaggtg ttctcagaag cttcagtagt taaagcgatt 960

gttgtcaaag acaaggcgga caagtactct cgaaaaaata ttgataaatt aacagaaatt 1020

gcaaaacaat acggtgccaa aggtttagca tggctaaaat atgtagataa tactatttca 1080

ggtccggttg ctaagttctt gacagctatt gaaggtaggt tgacagaagc tttgcagcta 1140

gagaataatg acttaattct atttgtagca gatagtcttg aagtagctaa cgagacttta 1200

ggagcgcttc gtacacgcat tgcaaaagaa cttgaactca ttgattattc taaatttaat 1260

ttcctatggg ttgttgattg gccaatgttt gagtggtcag aagaagaggg acgatatatg 1320

tcggctcatc atccattcac cttgccaaca gcggagacag cgcatgagtt ggaaggggat 1380

ttggctaaag tacgtgcagt tgcctatgat attgttttga atggatatga gttaggcggt 1440

ggtagcctac gtattaacca aaaagataca caagagcgta tgttcaaggc tttaggattt 1500

agcgctgaat cagctcagga acagtttggt ttcttacttg aagctatgga ttacggcttc 1560

ccaccacatg gtggattggc aataggcctt gaccgctttg ttatgttgtt agcaggcaag 1620

gacaatattc gagaagttat tgcattccct aaaaacaata aggctagtga tccaatgact 1680

caagctccta gtctcgtttc agaacaacaa ttagaagaat tatcacttac tgtagaaagt 1740

tatgaaaact ag 1752

SeqID 153

aaagaaaaag gaaaactaat aaagaaaaaa attctagaaa ataatactga tattattcca 60

aaaattatag aaaaaagtcc tcaaaatctt attcttacaa gtaattacaa cagagttaat 120

attgataaaa taaaaaatat aaaaaatttt gataaaggat tcgaactagg ctttccactt 180

tttgaaaaag gagaaattct gagaaaagaa ggcgaaataa ctagtgcaat tgaattattt 240

gataaagcac gtgaactagg atattttgta cccgccttat acaattctta tgcgatggct 300

tttaggaaaa taaagaatta tgatgatgaa attctaatac tacaagaagg aattgaaaga 360

ttcaaaaaaa gcaccctgtc atcaaatatt aacccaaaga caatcgatag atggagtact 420

cgtatttcta gagctaaaga tttaaaatgt aaataa 456

SeqID 154

ttgaatgtaa aaaaacatca tttagcttat ggagctatca ctttagtagc ccttttttca 60

tgtattttgg ctgtaatggt catctttaaa agttcacaag ttactactga atctttgtca 120

aaagcagata aagttcgcgt agccaaaaaa tcaaaaatga ctaaggcgac atctaaatca 180

aaagtagaag atgtaaaaca ggctccaaaa ccttctcagg catctaatga agccccaaaa 240

tcaagttctc aatctacaga agctaattct cagcaacaag ttactgcgag tgaagaggca 300

gctgtagaac aagcagttgt aacagaaaac acccctgcta ccagtcaggc acaacaagct 360

tatgctgtta ctgagacaac ttatagacct gctcaacacc agacgagtgg ccaagtattg 420

agtaatggaa atactgcagg ggctattggc tcagcagctg cagcacaaat ggctgctgca 480

acaggagtcc ctcagtctac ttgggaacat attattgccc gtgaatcaaa tggtaatcct 540

aatgttgcta atgcctcagg agcttcagga cttttccaaa cgatgccagg ttggggttca 600

acagctacag ttcaggatca agttaattca gctattaaag cttatcgtgc tcaaggttta 660

tcagcttggg gttactag 678

SeqID 155

atgtcaaatt gggatactaa gtttttgaaa aaaggtttta cttttgatga tgttctgctt 60

attcctgctg aaagccatgt tttaccaaat gaagttgata tgaagacaaa gttggctgat 120

aatttgacat taaacattcc aatcattaca gctgcaatgg atacagttac agatagtaag 180

atggctattg ctattgcacg tgctggtggg cttggtatca ttcataaaaa tatgtcaata 240

gttgatcaag cagaagaagt tcgtaaagtt aaacgctcag aaaatggtgt tattattgac 300

ccattcttct tgacaccaga taatactgtc tctgaagctg aggaattgat gcaaaactat 360

cgcattagtg gagttcctat tgtagagaca ctcgagaatc gtaaattagt tggaattatt 420

actaaccgtg atatgcgttt catttctgat tataaacaac taatctcaga gcacatgaca 480

agtcaaaatc ttgtaacagc tcctattggt acagatcttg aaactgcaga gcgtattctt 540

catgagcatc gtattgaaaa gctaccttta gtagatgatg agggacgtct ttcaggactt 600

attactatta aggatatcga aaaagttatt gaatttccta aggcagcaaa agatgaattt 660

ggacgtctct tagttgcagg tgcagtaggt gtaacctctg atacttttga acgtgcagaa 720

gctttgtttg aagcaggtgc agatgctatt gtcattgata cagcacatgg acattcagct 780

ggtgtgctcc gtaaaattgc tgaaattcgt gctcacttcc ctaatcgcac attgattgcg 840

ggaaatattg ctacagcaga aggagcgcgt gcactttatg atgcaggtgt ggatgtcgtt 900

aaagttggta ttggtccagg ttcaatttgt acgactcgtg tcgttgctgg ggtaggtgtt 960

cctcagatta cagctattta cgatgcagcg gctgttgcgc gtgagtacgg taagactatc 1020

attgctgatg gtggtattaa atattcaggt gatattgtaa aagcacttgc agcaggtgga 1080

aatgcagtta tgctaggttc aatgtttgca ggaacagatg aagcgccagg agaaacagaa 1140

attttccaag ggcgtaagtt caagacatac cgtggtatgg gctcaattgc agcaatgaaa 1200

aaaggttcaa gcgatcgtta tttccaaggt tctgtaaacg aggctaacaa attggttcca 1260

gaaggtattg aaggtcgtgt agcatacaaa ggttcagtag ccgatattgt tttccaaatg 1320

cttggtggca ttcgctctgg tatgggatat gtaggtgcag cgaacataaa agaacttcat 1380

gataatgctc aatttgttga aatgtcagga gctggcttga aagaaagcca tccccatgat 1440

gttcaaatta caaatgaggc accaaattat tcagtacact aa 1482

SeqID 156

atgacaatta accactataa gcttcgtata ccttactata caataagctt tctacttccc 60

ttcataatca tagtctgtat cctctttact aagaatattt attggggaag tccaacaacc 120

atattagcaa gcgatggctt tcatcaatat gttatcttta atcaagcatt aagaaatatt 180

cttcatggtt caaacagctt attctatacc tttacaagcg gtttaggttt aaatttttat 240

gctctgagta gttactattt gggaagtttt ttatccccaa tagtttattt ctttaaccta 300

aaaaatatgc ctgatgctat ctacctttta acaatatgta aaatagggtt gataggtttg 360

tcaatgtttg tcactctttg taaacgtcat tgtaaagtta atcgtgtctt acttttagta 420

atttctactt gctatagctt aatgagtttc tcaattagcc aaattgaaat taatatgtgg 480

ctagatgtat ttattctaat tccccttgtt gttttaggag ttgatcagct actatgggaa 540

agaaaaccta tactttattt cctctctctg acagccttat tcatccaaaa ctattatttt 600

ggttttatga ctgccatctt tactagcctt tacttcatcg tacaaataac ccgtaatact 660

gatagtaagg ttgcatttaa acaatttctt cattttacct ttttatctct tttagctggt 720

atgactagta gcattatgat tttgccaact tattttgatt taactactca tggtgaaaag 780

ttgacaaagg tatcgaagat gtttacagaa aattcttggt acatggattt gtttgctaaa 840

aatatgattg gagcctatga tacaacaaaa tttggctcta ttcctatgat ttatgtaggt 900

ttacttcctt tactcctttc tttactttac tttacaataa aggaagttcc tcgaaggact 960

agattagcat atggattttt aataatattt gttattgcta gtttttatat aacacctcta 1020

gatttatttt ggcaagggat gcatgctcca aatatgtttc tccatcgcta ctcttgggta 1080

ctttcagtac ttatctgtct gctagctgct gaatgccttg agtatttaga caacatttct 1140

tggaaaaaga ttttaggggt caatttaatt ttagtaagtg gtttcataat cacgttttta 1200

tttaaaaagc attatcatta ccttaatctt gaattacttc ttttaacctt aactttcctc 1260

tcagcttata ttattctaac tattagtttt gtcagtaagc aaatccctaa attagttttt 1320

tatccctttc ttattggttt tgttgtatta gagatgacat taaacacctt ttaccaacta 1380

aatagtctta acgatgaatg gatatttcca tctaggcaag gatatgctaa atataatcac 1440

agcattagca aacttgtcag aaagactgag agaaataact ctacattctt ccgtacagag 1500

cgctggttag gacaaaccgg caacgactct atgaaatata attataatgg tatttcacaa 1560

ttttcatcta ttagaaatcg ttcttcaagt caagtgcttg atagacttgg ctttaaatca 1620

gatggtacaa atttaaattt acgttatcaa aataatactt tgatcgcaga tagtctattt 1680

ggtgtgaagt ataaccttac agaatatcct tttgataaat ttggttttat caaaaaagct 1740

caagataaac aaaccattct ttacaaaaat cagtttgcca gtcaattagc tatactaacg 1800

aatcaagtat atcaagataa accatttact gtgaatactt tggataatca gacaactctt 1860

ttaaatcaat taagtggttt gaaagagacg tattttgagc atcttattcc aaatagcgtt 1920

tctgggcaaa ccactcttaa taaacaagta tttgttaaaa agaataaaca agggaatact 1980

gaaattacct ataacataac tatccctaaa aacagtcagt tatatgttag tatgccgttt 2040

ataaatttta ataatgagga aaataaaatt gttcaaatct ccgttaataa tggaccattt 2100

gtacctaata ctcttgataa tgcttattca ttttttaata ttggttcctt tgctgaaaac 2160

agtcgtatca aagtaaaatt tcaatttcca cacaatgatc aagtttcttt cccaattcct 2220

cacttttatg ggctcaagtt agaagcttat caaaaagcta tgactgttat taataaacga 2280

aaagtaaaag taagaactga tcacaataaa gttattgcta attatacaag tcctaataga 2340

agttctcttt tcttcactat tccctatgat aggggttgga aagcttatca aaataataaa 2400

gaaataaaaa ttttcaaagc tcagaaaggt tttatgaaaa taaatattcc taaaggaaaa 2460

ggcaaagtaa cattaatttt tatcccttac ggctttaaat ttggggtagg cctatctatt 2520

actggaatag tattatttac tgtctattat tttaaatttg gaaaaaataa aataggatga 2580

SeqID 157

tggtttatga agcactcggt tgggaagcgc cacaatttgg tcaca 45

SeqID 158

atgaaagatc acctaaggtg cttcctccat taccgatcca aaaacgttgg gaaaaatgga 60

acgagattga agggaggaaa gcctaatgac 90

SeqID 159

gacaaacagc ttttccaatg cgtgcagggcttccaaataa ggaacctcaa tggcaagaag 60

catgggatca agctgatatt tataaaaaac gtcaagcat 99

SeqID160

aacgaggaag ccatagatgg ggtgtccaag cttgggaaaa aaatcaccat tctaggaaat 60

aattcatacc tttcttcc 78

SeqID 161

atcaaacaat ggctattggt gatgaagaaa atgaccgtgc tatgcttgaa gttgttggaa 60

atcctgttgt tatgcaaaac ggcaatcctg aac 93

SeqID 162

aggcgcattt caaacaatct aggagtttta aaggaagcga ccgttataca cgccgta 57

SeqID 163

gagctctgcc taaagctgag caacgtcaag ccaatctata tgccttggca cttcgagcaa 60

atcagtttga aaaaacaggt ttcaagggtt tatcacgctt tattcgaa 108

SeqID 164

agaagctgct tcagtaggtg ctatccaaga tttaacagat tcagatatca ttttctcaaa 60

ccaccgtgga cacggacaaa ctattgcaaa aggtat 96

SeqID 165

agatggtttg gttttagata tgacgatggc agaaaattta gctttacaaa cttatta 57

SeqID 166

cccttaaaaa gtttgtcgat gacatcaaga atgaagctat tgaggttatc acaaagaaaa 60

gctatg 66

SeqID 167

aggcacttgc gaaagagaca aaagcaaaat catttaatga atttatgctt tctatgtgcc 60

gtgaaaaaat tgaaaaagga caatttaatt 90

SeqID 168

aagttattta ttcagcctat tcaggaaaatatgaagcagc ca atcgagat tatatcaaat 60

tactatgcaa agcttgggtt aatgaaaatc 90

SeqID 169

tatcaaacgt attcgaaaaa ggcaagaaaa tttggttccg tttggcttaa acctaag 57

SeqID 170

acgctgttat tccagatact tctcctgctt tctcatttga aaaagaacat ctttctggag 60

tggaatatgc acgctactta caat 84

SeqID 171

aagaagtttt acgaaataat attagcaacc aagtaccaca catcagtgtt caaatggagt 60

ttaaaactca agaacaagtt gacgaatacc aaaaaaatct cggaagcatc atccgggaaa 120

ttggagatac acttggaaca gcaactgaat tcaatgccaa aagtaacatt agcacttata 180

ctcttggtgg acaaatccaa cgcattattg 210

SeqID 172

ttaaagcaaa tgttccgtcg cgtattgcat ttgctgtttc aagtggtact gatagccgta 60

cgatccttga tgaaaatggt gctgaaaagc tcttgggacg gggtgacatg ctctttaagc 120

ctattgatga gaatcatcca gtacgactac aaggttcctt tatttcagat gatgatgttg 180

aaaggatcgt tggttttatc aaagaccaag ccgaggctga ctatgatgat gcctttgatc 240

ctggagaagt atctgaaaca gataacggct ctggtggtgg cggcggagta cctgaaagtg 300

atcctctttt tgaagaagcc aagggactcg ttt 333

SeqID 173

ggccggaaaa ttaattttag tcgtccatca cgatttgag 39

SeqID 174

agagttaagt ataaaaaatg ggaaccttta tcaatatccc gttcctgcta tgaaaaatct 60

tcgtcaacat caagcagaat t 81

SeqID 175

acttggtcaa gatgttgtat tcccaggtgt tactcgtggt gcaaaattag aagaagcaat 60

caatgctttg gaagatggac aagttctttt ggt 93

SeqID 176

aaggagaatt tcgttcaaag gttgaggagt ctattgaaag caatcagcaa aaatataatc 60

atttatagtc tagtttctct accattagct agacacttg 99

SeqID 177

agtataatca atattaaact agaaatatat gacatatgtc ctaaaactatctcaaatagg 60

gagtcaatac atggatatag aaaactactg ccaagactac tgtttggaga ctatctttcc 120

aaaacattat ttcaaggatt tacaagtaat caccctaact gccggccaat ccgtctg 177

SeqID 178

ctttctttca gaagaagtcg gcgaattggc tcaagccatt cgcaaata 48

SeqID 179

ttcattttca aggataggtt taaaatgaga cacttcacgg ac 42

SeqID 180

gggttgattt gtgcttgtaa agcctttatc tctgccatac tggctaacttattttgttcc 60

tctgttatcc ccattgccag ttgtcctgaa aatatttgcg ctaaaccaag gactaggttt 120

tcctccacct cagacattgt cttatctcct gcaaagtaca tttttaaggc acccacagtt 180

ttatcattta tttttagagg aactacaata gcagaattta ac 222

SeqID 181

tcctcctctt tggacaccca ttacaaacat agggagcttt tcttagtagc ggacagtcta 60

ggcagtcttt ggttgattct cttagttgct tatttcgtt 99

SeqID 182

cacctggtgc ccactcaata tcataaacag aatctacctcttgaatataa gaggctaaac 60

gctctagtcc ataag 75

SeqID 183

gcacacctac acgaagggta tcataagtat ccactaaaaa gacgcaatct ttatgagttt 60

ctgcgtaagc tt 72

SeqID 184

ccattgaaag agctccgcga acattatata attcctcgga aaattcgtct tcaaaacttt 60

ccccataga 69

SeqID 185

aagtatacta ttgcaggctc tcgtgtcacc cagcgtcgac catcattttc tgttactaaa 60

gta 63

SeqID 186

gcagcttttt catcaccagc agctaaaaac agagcattca ttacaacacg atcttca 57

SeqID 187

tccatggtta aacaaatatc gttctctgac catttgatat cacgttttga aggcttatgc 60

ttgaggcgat tttctgtgcg attccgtttg agacgctcac ttagctctgt ttctaattct 120

acaaataata cctcttggtt g 141

SeqID 188

taccacgttc tttctcgata tcatttgaat ccattgcacg ttcttcaagc tctttacgct 60

catcaagagt atgtgattgt tttaataatt catcaacgag agttgtttta ccgtggtcaa 120

cgtgggcaa 129

SeqID 189

gcacagcctt cttcaactag gatatcccct aaaattgatc gttttacacc taag 54

SeqID 190

tggaatagaa ctatcaattattttttgaat aacctgcaat tggtgactaa tatcaagttg 60

cccacgagaa aaattttcca gtcccaaaac aagtttctga gcttcatcaa aaac 114

SeqID 191

gtccaagaag gccataaaat tttccctctt caaaga 36

SeqID 192

caacaaagca ataacaccgc caattatagt cgcccctata aaccttggca taaaaagaaa 60

aatggattga gctgcagatg ctttagc 87

SeqID 193

ttctttgatg ataccaaagt taacattcat tggttggaaa tgtttgctgt ctgtatgtgt 60

aatataatgt ggcagtgcac caatagcagt agtttgtggg aaaactactt cactctcccc 120

attaaagcga cgggcagcat tgattcccgc tacaagacca cttgctgccg attctacata 180

accttcaaca ccggtcattt gtccagcaaa gaaaagattt gggttcttac gtgtagcaaa 240

ggtttggtc 249

SeqID 194

gtgtattcgt tacgcatagc gacattggaa ctagaacgaa acttgataga caagcgacgt 60

gggaaaccaa ccgattctag gtcaatagag tttttagagg ttagtttagc aatattatct 120

tgcaagaata agttaatacc tgacatcatg ctagagaaga catttccctt agtttcttct 180

gaagcgaagt cagaagacct aaagtttaaa tcagccatat ctctaaattt ggaaaattgt 240

tct 243

SeqID 195

ggccatagca atggcattaa agagggaaat ggaggtatct tcccagtatt ttgcattgcc 60

attaccgcta ccaggtttcg ttttacgata aatggcttct gcaaccgcat taacccttgt 120

ctgtgtcatt tcatagtaac ccttcttagc tgctgcaatc gcaagagc 168

SeqID 196

agaatagaga aggctatcgt ccctaaaccg gccattagtg ttctgatgat aatctcaagg 60

atggtattag gaacaccaga aaaagaacct gtcatttgtt ctgcaaacgg aataatttca 120

attgtaatag ccacacctgc tgatatagaa atacaaatga gtaaagcaga cataatcaaa 180

gctaaacctg ttgaaaaatt attctgtgaa aattctctca ccgaattaac aaaggcagcc 240

cctggcacca taaccattag tgcccctaat ataataatgc tacgatgctc acctaatcct 300

gaaaaatata ataaattagc acttaaagcc actacagaac tacctagaat ggtcaaaaga 360

aatcctgtat gtatacgact ttccatcaaa ttcagaaaat atcctaaaat taagcctgtt 420

acagcagcgg aaaatgaatc taataaggaa ctacctaagg ctaaagaaaa actacccgct 480

ccacaaaaa 489

SeqID 197

ccatattctg taacatatgc tgctccagta atttgtaatg gagcggattt agcacgacga 60

acagcatatg ctatgtcgtc atcagttaca aaaccttctg atgtagaaga attagttacc 120

ataggtgata gaacaaaacg attttctagg ctcaatccat tgggaagtat taatggacga 180

aataaattac cttggacatt attcatctct gctcaccgtc cccttattag ccatagactt 240

agcctgaaat ctatcaaact ctgcaac 267

SeqID 198

gccaaccata atgacaaaaa taatacgata agcgttaata aatttagtgc caaa 54

SeqID 199

tccttctgcg tattttgttg ccattccaaa aaaggcagct acccacatcc aaaacaaagc 60

tcctggtcct ccagatttaa tagccgtagc cacaccaacg atattacctg tccctaccgt 120

tgcagcaaga gcagttgcaa gggcagcata act 153

SeqID 200

acattaggca tttcttttaa aataagaggg tgccatttct ccgcaaaacc ttttctaggg 60

ggtaaatctc ctgattttcc tttgcctggg 90

SeqID 201

tttaatattt ctaatagttc cttaggtttt ggtacttctg ctaaatcagc aagtacttcc 60

tcagctaatt cttccttaat aatttcttgt gataaggcca cacattcatt acaaatgaaa 120

acaccattac ctgcaataat tttttttact tcatcttggc ttttgccaca aaatgaacaa 180

SeqID 202

ggtgtttata aaaagcagag cctaagcctg attttccatc agattcacct gattgtttat 60

cattttgatt ttttgcatga taggtttgtg ccttattatc gagaa 105

SeqID 203

attttcttac gatcagtatg tggaaattca cgacaagctt taggtctgac atcgtatata 60

ctacataagt tatcatcgcc taaaaacgga caaggcatcg ctttaaatac tttatctcca 120

tcttcatcta cttctaaaaa taaatcttca aaagccgata acttcatccg gaaatgttta 180

gaaatcctag aaatatcagc ttctgtaaat agaggaccca aagttttaca acaattagca 240

catgctgtac aatcaatttc t 261

SeqID 204

agtagaataa gtacaataac aataccaata aggataagcc caactttttt atatagagag 60

gacttttctt caggacttaa aggatcgttt ggtctgagac tctcttcagt caaatatttt 120

tttccatcta aggtatattg gaggagacca aagaacatac caatagcagc aagtgagaaa 180

ccaagatgga aatttacttc ttgtccgagg taccccacta caatgggtga aataaaagca 240

ccaaggttaa taccaaaaac aaagatacta aaaccagcat cacgacgaga atcattttct 300

ccatataaat taccaaccat ctctgataca tttggtttta atagtccagt accaaggata 360

attagagcaa tcgaaatgaa tagagctgtt 390

SeqID 205

tcttttcctc gttcaaataa tttatgtgct tttaaagata agttcaaatg tcgcacaagt 60

tctcgattag actgttta 78

SeqID 206

atccattcat ttcatagaag gaaattaaat catcatggca agttaaactt ataccatctc 60

gttcttgaga aacaaccagg tctttcatcg ctgctaataa tgctgtacca atcccctgac 120

ctt 123

SeqID 207

ctgtcttttt cgatagtatc ccatttatta actacaataa taataccttt accagcttca 60

tgtgcaaacc ctgcaatacg cttgtcatac tcacgaatac cttcctctgc attaatcacc 120

ataagaacaa catctgagcg gtcgatggcg cgcatagaac gcataacaga atatttctct 180

gtgttttcat ataccttacc agatttacgc ataccagcag tatcaatcat agtatactct 240

tgcccctggc tatcaacgaa attagtatca atagcatcgc gcgtagtacc agcaactggg 300

ctagcaataa cacggtcttc cccaaggatg gcattaatta aactagattt cccaacattt 360

ggacgtccaa tcaaactaaa acgaataata tctggatttt cattttcctc ttctaccgga 420

agattttcca caatagcatc caatatatca ccagtaccaa tacca 465

SeqID 208

tgcccatcct cagatatagc tggaattaat tttaccaaaa atagcggaaa aatagataaa 60

caaaccgtta ca 72

SeqID 209

ccctttctca gaaatcattt ccataacaac gagaccagca ccaaattctt tagcgatagt 60

acgaaaagca gaattggtaa tacctgccatcggtgc 96

SeqID 210

gcgtcgacag gatcagcagc gaatccacag actacgccag gaaaactatt acaagcaagc 60

atcgctccaa ttcctgtccc acagcctgta ataacaaaat ctgcagcacc gctattaagt 120

aaaatagcag tcagtaaacc attttgtaca 150

SeqID 211

gcaatgccta aacgttgctt cataccaccg gagtatgatg aaatcttctt tttacctaca 60

tcgcctaaac caacaagctc tagtaatcta ggaatttctt gtttagcttt ctttttatca 120

acacctttta aggtagcgat a 141

SeqID212

cttttactga ttttaaaaaa tccagatact acacataaca ccaccataaa aagacaacct 60

aacaaacccc ctaaagtttt acgaaaaaga actgaaaaaa agagt attaa gcttatgaaa 120

agaataccga taaaccatgt tgcaccg 147

SeqID 213

aatttcaaag cacgaccacc aggtgttgtt tcagggttac caaacataac gcctactttt 60

tcccttaact ggttgataaa aattgcgatt gtctttgtct tgttaatgga tgctgacaat 120

ttacgcattg ct 132

SeqID 214

aaaagagtag ggatttgcca catatatcat gtatcccaat ccagctgttt ccacaacaat 60

aaatttagcc gttattttac tcaattttcc tttaatataa tcgtacattc tattttccta 120

tctcaatatt tttcattttg g 141

SeqID 215

aattcaacga caagtgcttc attgatttct gggttaattt catcgcgttc tggaaggcga 60

gtcaatgaac cttcaagttt ttcagcatca aatgatacga aagctggacg tcctaaagta 120

gcttcaacag cttcaaggat tgcaggtact ttcattgatt tttcacgaac tgaaataact 180

tgtcctggag taacgcggtatgatgggata tcaacacgtt taccatcaac aaggata 237

SeqID 216

acaagaccct tagtagaaat cttagttgat aaactagcgc catataagga agctaacttt 60

tctcgaaatt cttgtacctt aggatactta gcatttgcag taactaacat t 111

SeqID 217

agaactcgaa atgcaattag atatcctaaa gcaagcagcg gtgattatgg cacaaaaagg 60

gaaataatca ctgctaataa ggataaatac agcatttcaa agatgtgtcg ctggctgaat 120

atgccacgct caagttatta ctatcaagcc gtggagtcag tatctaaaac ggagtttgaa 180

gaaactatta aaagaatttt tctcgatagc gagtctagat acggatccag aaaaatcaaa 240

atatgcttga ataacgaagg tatcacactt tcacgtcgtc ggattcgacg cattatgaag 300

cgactcaatt tggtttctgt ttatcagaaa gccaccttca aaccacattc tagaggcaag 360

aatgaagccc ctattcccaa ccacttagac aggcaattta agcaagaaag accactacaa 420

gccttagtca ctgacttaac ctatgttcgt gtaggcaatc gttgggctta tgtttgcctc 480

atcattgacc tatacaaccg tgaaatcatc ggcctgtctc ttggttggca caagaccgct 540

gaactcgtta agcaagccat acaaagcatc ccttacgccc tgaccaaagt caagatgttc 600

cattcagatc gtggcaaaga gtttgataat cagttaattg atgaaatatt ggaagccttt 660

ggaatcacac gttcgcttag tcaggctggt tgtccttatg acaatgccgt agctgaaagt 720

acgtatcgtg ctttcaaaat tgaatttgtt tatcaagaaa cctttcaatc gctggaagaa 780

ctagctctta agactgaaaa ggcaacactt ttctgtacaa catttataaa gtgttgcctt 840

ttcaggtttt taccaatgct a 861

SeqID 218

MKKVLTFLLCSLYFVSIPAISTEEPLTLSQNRRYALTQTVVDKEMYFDAIPERPTTKIEISSFQDEALTITGETLVP

NTLLSIVSLTINSNGIPVFTLSNGQFIKASREAIFNDLVSKQQSVSLDYWLKPSFVTYEAPYTNGVSEVKNNLKPYS

RVHLVEQAETEHGIYYKTDSGFWISVEDLSVADNRMAKVQEVLLEKYNKDKYGIYIKQLNTQTVAGINIDRSMYSAS

IAKLATLYASQEQVKLGKLSLDSKFEYKDNVNQFPNSYDPSGSGKLEKKADHKLYTVKKLLEATAKESDNVATNMLG

YYVNNQYDSMFQTQVDTISGMHWDMKKRQISPQAAGKMMEAIYYQNGDIVNYLSKTDFDNTRIPKNIPVKVAHKIGD

AYDYKHDAAIVYAEQPFIMIIFTDKSSYDDITKIADDVYQVLK

SeqID 219

MKKRILSAVLVSGVTLGTAAVTVNADDFDSKIAATDSVINTLSGQQAAAQNQVTAIKGQVGALESQQSELEAQNAQL

EAVSQQLGQEIQTLSNKIVARNESLKKQVRSAQKGNLTNYINTILNSKSVSDAVNRVVAIREVVSANEKMLAQQEAD

KAALEAKQIENQNAINTVAANKQAIENNKAALATQRAQLEAAQLELSAQLTTVQNEKASLIQAKAQAEEAARKAAEA

QAAAEAKAQAEAKAQAESVAKAQAAAQVESATAPTETVQTQPRTEIKPSNLTATSSATTVATTTATATNEPKVTQPS

VVTKAVEAPKAVVSSTPRAVSKPVVRSYDSSNTYPMGQCTWGAKSMASWVGNYWGNANQWGASARAAGYSVGTTPRV

GAVAVWPYDGGGYGHVAVVTSVANNSSIQVMESNYAGNMSIGNYRGSFNPSASGSVYYIYPN

SeqID 220

MNKRIFVEKKADFDIKSASLVKELTHNLQLTSLKDLRIVQVYDVFNLAEDLLARAEKHIFSEQVTDCLLTETEITAE

LDKVAFFAIEALPGQFDQRAASSQESLLLLGSDSQVKVNTAQLYLVNKDIAEAELEAVKNYLLNPVDSRFKDITLPL

EEQAFSVSDKTIPSLDFFETYKADDFAAYKAEQGLAMEVDDLLFIQDYFKSIGRVPTETELKVLDTYWSDHCRHTTF

ETELKNIDFSASKFQKQLQATYDKYIAMRDELGRSEKPQTLMDMATIFGRYERANGRLDDMEVSDEINACSVEIEVD

VDGVKEPWLLMFKNETHNHPTEIEPFGGAATCIGGAIRDPLSGRSYVYQAMRISGAGDITTPIAETRAGKLPQQVIS

KTAAHGYSSYGNQIGLATTYVREYFHPGFVAKRMELGAVVGAAPKENVVREKPEAGDVVILLGGKTGRDGVGGATGS

SKVQTVESVETAGAEVQKGNAIEERKIQRLFRNGNVTRLIKKSNDFGAGGVCVAIGELADGLEIDLDKVPLKYQGLN

GTEIAISESQERMSVVVRPSDVDTFIAACNKENIDAVVVATITAKPNLVMTWDGETIVDLERRFLDTNGVRVVVDAK

VVDKDLTVPEVRTTSAETLEADTLKVLSDLNHASQKGLQTIFDSSVGRSTVNHPIGGRYQITPTESSVQKLPVQHGV

TTTASVMAQGYNPYIAEWSPYHGAAYAVIEATARLVATGADWSRARFSYQEYFERMDKQAERFGQPVSALLGSIEAQ

IQLGLPSIGGKDSMSGTFEELTVPPTLVAFGVTTADSRKVLSPEFKAAGENIYYIPGQAISEDIDFDLIKANFSQFE

TIQAQHKITAASAVKYGGVLESLALMTFGNRIGASVEIAELDSSLTAQLGGFVFTSAEEIADSVKIGQTQAAFTLTV

NGNDLAGASLLSVFEGKLEEVYPTEFEQADALEEVPAVVSDTVIKAKETIEKPVVYIPVFPGTNSEYDSAKAFEQVG

ASVNLVAFVTLNEAAIADSVDTMVANIAKANIIFFAGGFSAADEPDGSAKFIVNILLNKKVRAAIDSFIEKGGLIIG

ICNGFQALVKSGLLPYGNFEEAGETSPTLFYNDANQHVAKMVETRIANTNSPWLVGVEVGDIHAIPVSHGEGKFVVS

ASEFAELRDNGQIWSQYVDFDGQPSMDSKYNPNGSVNAIEGITSKNGQIIGKMGHSERWEDGLFQNIPGNKDQALFA

SAVKYFTGK

SeqID 221

MKMNKKVLLTSTMAASLLSVASVQAQETDTTWTARTVSEVKADLVKQDNKSSYTVKYGDTLSVISEAMSIDMNVLAK

INNIADINLIYPETTLTVTYDQKSHTATSMKIETPATNAAGQTTATVDLKTNQVSVADQKVSLNTISEGMTPEAATT

IVSPMKTYSSAPALKSKEVLAQEQAVSQAAANEQVSPAPVKSITSEVPAAKEEVKPTQTSVSQSTTVSPASVAAETP

APVAKVAPVRTVAAPRVASVKVVTPKVETGASPEHVSAPAVPVTTTSPATDSKLQATEVKSVPVAQKAPTATPVAQP

ASTTNAVAAHPENAGLQPHVAAYKEKVASTYGVNEFSTYRAGDPGDHGKGLAVDFIVGTNQALGNKVAQYSTQNMAA

NNISYVIWQQKFYSNTNSIYGPANTWNAMPDRGGVTANHYDHVHVSFNK

SeqID 222

MLKHFGSKVRNLRVTRNITREDFCGDETELSVRQLARIESGQSIPNLTKAHYLAKQLNVKLDILTGGESLELPKRYK

ELKYKLLRTPTYGDANRLAVREAYFDEIYEVFYEELPEDERLIIDCMQSKLDVHFSVNDNFGITILHDYFDQIKKKK

EYTTNDFVMIDLYLLCFSINYGMKSLYSLENYHFIMSKLLEQDNLLPEDNFQLNNVLLNHVELAFQFKQKKYVQQII

HRSNAIMTEIHDFQKRPILSLIEWKYLLIIEKDRTKAETCFKQSILFAELIGDLYLKGKLIEEWNKDLT

SeqID 223

MTEKTKAVETTDVALAIDTLVQNGLKALDEMRQLNQEQVDYIVAKASVAALDAHGELALHAVEETGRGVFEDKATKN

LEACEHVVNNMRHTKTVGVIEEDDVTGLTLIAEPVGVVCGITPTTNPTSTAIFKSLISLKTRNPIIFAFHPSAQESS

AHAARIVRDAAIAAGAPENCVQWIEQPSIDATNALMNHDGIATILATGGNAMVKAAYSCGKPALGVGAGNVPAYVEK

SANIRQAAHDIVMSKSFDNGMVCASEQAVIIDKEIYKEFVEEFKSYHTYFVNKKEKALLEEFCFGAKANSKNCAGAK

LNPNIVGKSAVWIAEQAGFTVPEGTNILAAECTEVSEKEPLTREKLSPVIAVLKAESTEDGVEKARQMVEFNGLGHS

AAIHTKDADLAREFGTRIRAIRVIWNSPSTFGGIGDVYNAFLPSLTLGCGSYGRNSVGDNVSAINLLNIKKVGRRRN

NMQWFKVPSKTYFERDSIQYLQKCRDVERVMIVTDHAMVELGFLDRIIEQLDLRRNKVVYQIFAEVEPDPDITTVMK

GTDLMRTFKPDTIIALGGGSPMDAAKVMWLFYEQPEVDFHDLVQKFMDIRKRAFKFPELGKKTKFVAIPTTSGTGSE

VTPFAVISDKANNRKYPIADYSLTPTVAIVDPALVMTVPGFIAADTGMDVLTHATEAYVSQMANDYTDGLALQAIKI

VFDYLERSVKDADFEAREKMHNASTMAGMAFANAFLGISHSMAHKIGAQFHTVHGRTNAILLPYVIRYNGTRPAKTA

TWPKYNYYRADEKYQDIAKLLGLPAATPEEAVESYAKAVYDLGTRLGIKMNFRDQGIDEKEWKEKSRELAFLAYEDQ

CSPANPRLPMVDHMQEIIEDAYYGYEERPQRRK

SeqID 224

VGIKVYKPTTNGRRNMTSLDFAEITTNTPEKSLLVSLKNKAGRNNNGRITVRHQGGGHKRHYRLIDFKRNKDGVEAV

VKTIEYDPNRTANIALVHYTDGVKAYILAPKGLEVGQRIISGPEADIKVGNALPLANIPVGTVIHNIELQPGKGAEL

IRAAGASAQVLGQEGKYVLVRLQSGEVRMILGTCRATIGTVGNEQQSLVNIGKAGRNRWKGVRPTVRGSVMNPNDHP

HGGGEGKAPVGRKAPSTPWGKPALGLKTRNKKAKSDKLIVRRRNQK

SeqID 225

MIEFEKPIITKIDENKDYGRFVIEPLERGYGTTLGNSLRRVLLSSLPGAAVTSIKIDGVLHEFDTIPGVREDVMQII

LNVKGLAVKSYVEDEKIIELDVEGPAEITAGDILTDSDIEIVNPDHYLFTIAEGHSLKATMTVAKNRGYVPAEGNKK

DDAPVGTLAVDSIYTPVKKVNYQVEPARVGSNDGFDKLTIEIMTNGTIIPEDALGLSARVLIEHLNLFTDLTEVAKA

TEVMKETEKVNDEKVLDRTIEELDLSVRSYNCLKRAGINTVFDLTEKTEPEMMKVRNLGRKSLEEVKIKLADLGLGL

KNDK

SeqID 226

MKKIRLSKFIKMIVVILFLISVAASFYFFHVAQVRDDKSFISNGQRKPGNSLYAYDKSFDKLLKQKIEMTNQNIKQV

AWYVPAAKKTHKTAVVVHGFANSKENMKAYGWLFHKLGYNVLMPDNLAHGESHGQLIGYGWNDRENIIKWTEMIVDK

NPSSQITLFGVSMGGATVMMASGEKLPSQVVNIIEDCGYSSVWDELKFQAKEMYGLPAFPLLYEVSTISKIRAGFSY

GQASSVEQLKKNNLPALFIHGDKDNFVPTSMVYDNYKATAGKKELYIVKGAKHAKSFETEPEKYEKRISSFLKKYEK

SeqID 227

MSKVRGFELVSQFSNKKLLPKRETAHAAGYDLKVAKKTVIEPGEITLVPTGIKAHMQPGEVLYLYDRSSNPRKKGIV

LINSVGVIDGDYYNNQVNEGHIFAQMQNITDQAVILEEGERIVQAVFAPFLLADDDQATGMRTGGFGSTGK

SeqID 228

MKFGKKLGFLALLMSIVLILGACGKTGLGNSTGNSTKNVTKKSAKNLKLGVSISTTNNPYFVAMKDGIDKYASNKKI

SIKVADAQDDAARQADDVQNFISQNVDAILINPVDSKAIVTAIKSANNANIPVILMDRGSEGGKVLTTVASDNVAAG

KMAADYAVKKLGKKAKAFELSGVPGASATVDRGKGFHSVAKSKLDILSSQSANFDRAKALNTTQNMIQGHKDVQIIF

AQNDEMALGAAQAVKSAGLQNVLIVGIDGQPDAHDAIKKGDISATIAQQPAKMGEIAIQAAIDHYKGKKVEKETISP

IYLVTKDNVEKYNW

SeqID 229

MGKEKLILAYSGGLDTSVAIAWLKKDYDVIAVCMDVGEGKDLDFIHDKALTIGAIESYILDVKDEFAEHFVLPALQA

HAMYEQKYPLVSALSRPIIAQKLVEMAHQTGATTIAHGCTGKGNDQVRFEVAIAALDPELKVIAPVREWKWHREEEI

TFAKANGVPIPADLDNPYSIDQNLWGRANECGVLENPWNQAPEEAFGITKSPEEAPDCAEYIDITFQNGKPIAINNQ

EMTLADLILSLNEIAGKHGIGRIDHVENRLVGIKSREIYECPAAMVLLAAHKEIEDLTLVREVSHFKPILENELSNL

IYNALWFSPATKAIIAYVKETQKVVNGTTKVKLYKGSAKVVARHSSNSLYDENLATYTAADNFDQDAAVGFIKLWGL

PTQVNAQVNKG

SeqID 230

MAKVCYFTGRKTVSGNNRSHAMNQTKRTVKPNLQKVTVLIDGKPKKVWVSARALKSGKVERV

SeqID 231

MAKQKNNWRRVGVGVLTLASVATLAACGSKSASQDSNGAINWAIPTEINTLDLSKVTDTYSNLAIGNSSSNFLRLDK

DGKTRPDLATKVDVSKDGLTYTATLRKGLKWSDGSKLTAKDFVYSWQRLVDPKTASQYAYLAVEGHVLNADKINEGQ

EKDLNKLGVKAEGDDKVVITLSSPSPQFIYYLAFTNFMPQKQEVVEKYGKDYATTSKNTVYSGPYTVEGWNGSNGTF

TLKKNKNYWDAKNVKTKEVRIQTVKKPDTAVQMYKRGELDAANISNTSAIYQANKNNKDVTDVLEATTAYMQYNTTG

SVKGLDNVKIRRALNLATNRKGVVQAAVDTGSKPAIAFAPTGLAKTPDGTDLAKYVAPGYEYNKTEAAKLFKEGLAE

SGLTKLKLTITADADVPAAKNSVDYIKSTWEAALPGLTVEEKFVTFKQRLEDSRKQNFDIVVSVWGGDYPEGSTFYG

LFKSDSQNNDGKFANKDYDAAYNKAISEDALKPEESAKDYKEAEKILFEQGAYNPLYFRSGKGLQNPKLKGVIRNTT

GLSIDFTHAYKK

SeqID 232

MELLKTPIFGICFSLILYTIGQHLFKKSKGFFLLQPLFFAMVSGIVILWLMSKGLGTDVKTFYTQAYKPGGDLIFWF

LNPATIAFAVPLYKKNDVVKKYWVEILSSLVIGMIVSLMLIVAISKMVGLSQVGIASMLPQAATTAIALPITAAIGG

NTAVTAMACILNAVIIYALGKKLVSFFHLNDSKIGAGLGLGTSGHTVGAAFALELGELQGAMAAIAVVVIGLVVDLV

IPIFSHLIGLL

SeqID 233

VTKYLKYISFVALFLASIFLVACQNQNSQTKERTRKQRPKDELVVSMGAKLPHEFDPKDRYGIHNEGNITHSTLLKR

SPELDIKGELAKKYKISKDGLTWSFDLNDDFKFSNGEPVTADDVKFTYDMLKADGKAWDLTFIKNVEVVGKNQVNIH

LTEAHSTFTAQLTEIPIVPKKHYNDKYKSNPIGSGPYMVKEYKAGEQAIFVRNPYWHGKKPYFKKWTWVLLDENTAL

AALESGDVDMIYATPELASKKVKGTRLLDIASNDVRGLSLPYVKKGVVKNSPDGYPVGNDVTSDPAIRKALTIGLNR

QKVLDTVLNGYGKPAYSIIDRTPFWNPKTAIKDNKVAKAKQLLTKAGWKEQADGSRKKGNLKAEFDLYYPTNDQLRA

NLAVEVAEQAKALGITIKLKASNWDEMATKSHDSALLYAGGRHHAQQFYESHYPSLAGKGWTNITFYNNPTVTKYLD

KAMTSPDLDKANKYWKLAQWDGKTGASTLGDLPNVWLVSLNHTYIGDKRINVGKQGVHSHGHDWSLLTNIAEWTWDE

SAK

SeqID 234

MGQEPIIEYQNINKVYGENVAVEDINLKIYPGDFVCFIGTSGSGKTTLMRMVNHMLKPTNGTLLFKGKDISTINPIE

LRRRIGYVIQNIGLMPHMTIYENIVLVPKLLKWSEEAKRAKARELIKLVELPEEYLDRYPSELSGGQQQRIGVIRAL

AADQDIILMDEPFGALDPITREGIQDLVKSLQEEMGKTIILVTHDMDEALKLATKIIVMDNGKMVQEGTPNDLLHHP

ATSFVEQMIGEERLLHAQADITPVKQIMLNNPVSITAEKTLTEAITLMRQKRVDSLLVTDNGKLIGFIDLESLSSKY

KKDRLVSDILKHTDFYVMEDDLLRNTAERILKRGLKYAPVVDHENNLKGIVTRASLVDMLYDIIWGDTETEDQ

SeqID 235

MKIDKKEFLALIASIILLIFASVTFFLFKDHGTTQMDTVESSVNHVSDSQLTEAQDMLDKFEKKPSEKLLKDVELAL

NKLSNSSKKEALQKRFKKAKDKYLKDEADKKATKDATDLVEILEQAPSEENVLKAEAAVNKLTVKESKEALQKRIDT

VKTQYGLIGNQTPSSSVAETTEQGTANPASQDTSSYVNQNVAPTYEQPQTNNTPVTPGVNNTVPTPGTGTAPATNGT

GVAQ

SeqID 236

MTKDLLLELGLEELPAYVVTPSEKQLGQKMVKFLEDHRLSFETVQTFSTPRRLAVRVKGLADQQTDLTEDFKGPSKK

IALDAEGNFSKAAQGFVRGKGLSVDDIEFREVKGEEYVYVTKHETGKAAIDVLASVTEVLTELTFPVNMHWANNSFE

YIRPVHTLVVLLDDQALELDFLDIHSGRISRGHRFLGSDTEILSASSYEDDLRQQFVIADAKERQQMIVDQIHAIEE

KENISVEIDEDLLNEVLNLVEYPTAFLGSFDEKYLDVPEEVLVTSMKNHQRYFVVRDRDGKLLPNFISVRNGNAEHI

ENVIKGNEKVLVARLEDGEFFWQEDQKLNIADLVEKLKQVTFHEKIGSLYEHMDRVKVISQYLAEKADLSDEEKLAV

LRAASIYKFDLLTGMVDEFDELQGIMGEKYALLAGEQPAVAAAIREHYMPTSADGELPETRVGAILALADKFDTLLS

FFSVGLIPSGSNDPYALRRATQGIVRILEAFGWDIPLDELVTNLYGLSFASLDYANQKEVMAFISARIEKMIGSKVP

KDIREAVLESDTYIVSLILEASQALVQKSKDAQYKVSIESLSRAFNLAEKVTHSVSVDYSLFENNQEKALYQAILSL

ELTEDMHDNLDKLFALSPIINDFFDNTMVMTDDEKMKQNRLALLNSLVAKARTVAAFNLLNTK

SeqID 237

MTFDTIDQLAVNTVRTLSIDAIQAANSGHPGLPMGAAPMAYVLWNKFLNVNPKTSRNWTNRDRFVLSAGHGSALLYS

LLHLAGYDLSIDDLKQFRQWGSKTPGHPEVNHTDGVEATTGPLGQGIANAVGMAMAEAHLAAKFNKPGFDLVDHYTY

TLHGDGCLMEGVSQEAASLAGHLKLGKLVLLYDSNDISLDGPTSQSFTEDVKGRFESYGWQHILVKDGNDLEAIAAA

IEAAKAETDKPTIIEVKTIIGFGAEKQGTSSVHGAPLGAEGITFAKKAYGWEYPDFTVPAEVVARFASDLQARGAKA

EEAWNDLFAKYEVEYPELAAEYKEAFAGQAETVELKAHDLGSSVASRVSSQQAIQQLSTQLPNLWGGSADLSASNNT

MVAAETDFQASNYAGRNIWFGVREFAMAAAMNGIALHGGTRVYGGTFFVFSNYLLPAVRMAALQNLPTVYVMTHDSI

AVGEDGPTHEPIEQLASVRSMPNLNVIRPADGNETNAAWQRAVSETDRPTMLVLTRQNLPVLEGTSELAQEGVNKGA

YILSEAKGELDGIIIATGSEVKLALDTQDKLESEGIHVRVVSMPAQNIFDEQEASYQEQVLPSAVTKRLAIEAGSSF

GWGKYVGLNGLTLTIDTWGASAPGNRIFEEYGFTVENAVSLYKEL

SeqID 238

MTLQDQIIKELGVKPVINPSQEIRRSVEFLKDYLLKHSFLKTYVLGISGGQDSTLAGRLAQLAVEELRADTGENYQF

IAIRLPYGIQADEEDAQKALDFIKPDIALTINIKEAVDGQVRALNAAGVEITDFNKGNIKARQRMISQYAVAGQYAG

AVIGTDHAAENITGFFTKFGDGGADLLPLFRLNKSQGKQLLAELGADKALYEKIPTADLEENKPGIADEIALGVTYQ

EIDAYLEGKVVSDKSRGIIENWWYKGQHKRHLPITIFDDFWK

SeqID 239

IKKESVIKLLKYAFGIIMGFIILAIVIGGLLFAYYVSRSPKLTDQALKSVNSSLVYDGNNKLIADLGSEKRESVSAD

SIPLNLVNAITSIEDKRFFKHRGVDIYRILGAAWHNLVSSNTQGGSTLDQQLIKLAYFSTNKSDQTLKRKSQEVWLA

LQMERKYTKEEILTFYINKVYMGNGNYGMRTTAKSYFGKDLKELSIAQLALLAGIPQAPTQYDPYKNPESAQTRRNT

VLQQMYQDKNISKKEYDQAVATPVTDGLKELKQKSTYPKYMDNYLKQVISEVKQKTGKDIFTAGLKVYTNINTDAQK

QLYDIYNSDTYIAYPNNELQIASTIMDATNGKVIAQLGGRHQNENISFGTNQSVLTDRDWGSTMKPISAYAPAIDSG

VYNSTGQSLNDSVYYWPGTSTQLYDWDRQYMGWMSMQTAIQQSRNVPAVRALEAAGLDEAKSFLEKLGIYYPEMNYS

NAISSNNSSSDAKYGASSEKMAAAYSAFANGGTYYKPQYVNKIEFSDGTNDTYAASGSRAMKETTAYMMTDMLKTVL

TFGTGTKAAIPGVAQAGKTGTSNYTEDELAKIEATTGIYNSAVGTMAPDENFVGYTSKYTMAIWTGYKNRLTPLYGS

QLDIATEVYRAMMSYLTGGYSADWTMPEGLYRSGSYLYINGTTTTGTYSSSVYKNIYQNSGQSSQSSSSTSSEKQKE

DKNTANDANSSSPQVETPNNGNATTPNNSNQTVPGTGHGNGNGNNNTVPNGN

SeqID 240

MLDLKRIRTDFDVVAKKLATRGVDQETLTTLKELDIKRRELLIKAEEAKAQRNVASAAIAQAKRNKENADEQIAAMQ

TLSADIKAIDAELADVDANLQSMVTVLPNTPADDVPLGADEDENVEVRRWGTPREFDFETKAHWDLGESLGILDWER

GAKVTGSRFLFYKGLGARLERAIYSFMLDEHAKEGYTEVIPPYMVNHDSMFGTGQYPKFKEDTFELADSPFVLIPTA

EVPLTNYYRDEIIDGKELPIYFTAMSPSFRSEAGSAGRDTRGLIRLHQFHKVEMVKFAKPEESYQELEKMTANAENI

LQKLNLPYRVITLCTGDMGFSAAKTYDLEVWIPAQNTYREISSCSNTEDFQARRAQIRYRDEVDGKVRLLHTLNGSG

LAVGRTVAAILENYQNEDGSVTIPEVLRPYMGNIDIIKPN

SeqID 241

VTISNQELTLTPLRGKSGKAYIGTYPNGERVFVKYNTTPILPALAKEQIAPQLLWARRTSNGDMMSAQEWLDGRTLT

KEDMGSKQIIHILLRLHKSRPLVNQLLQLGYKIENPYDLLMDWEKQTPIQIRENTYLQSIVTELKRSLPEFRTEVAT

IVHGDIKHSNWVITTSGLIYLVDWDSVRLTDRMYDVAYILSHYIPQKHWKDWLSYYGYKDNEKVWSKIIWYGQFSYL

SQIIKCFDKRDMEHVNQEIYELRKFRELIKKHNAS

SeqID 242

MKISQYNKWSIRRLKVGAASVMIASGSIVALGQSHIVSADEMSQPKTTITAPTANTSTNVESSTDKALSKVTTMETS

SEMPKMQNMAKVEKTSDKPMMVATSVRKMMATPTPVAMTKTTSVDEVKKSTDTAFKQTVDVPAHYVNAAKGNGPFLA

GVNQTIPYEAFGGDGMLTRLILKSSEGAKWSDNGVDKNSPLLPLKGLTKGKYFYQVSLNGNTTGKEGQALLDQIKAN

DKHSYQATIRVYGAKDGKVDLKNMISQKMVTINIPHITTDMEVKNSLKMAFKEKVDVPAKYVSAAKAKGPFLAGVNE

TIPYEAFGGDGMLTRLILKASEGAKWSDNGVDKNSPLLPLKDLTKGKYFYQVSLNGNTAGKKGQALLDQIKANGSHT

YQATITIYGTKDGKVDMNTILGQKTVMIHTNVAKKDMNSTSMMMKKDKMTMPMKKEMTSSKINTGMMMSNNKMSANM

QMSSQAKSNDKAGKKMSMMSKNLPNTGETKQQNVGVLGMLSLAFATGLTALGLKKSKQR

SeqID 243

MTHITFDYSKVLGQFVGEHELDYLQPQVSAADAFLRQGTGPGSDFLGWMDLPENYDKEEFSRIQKAAEKIKSDSEVL

VVIGIGGSYLGAKAAIDFLNNHFANLQTAEERKAPQILYAGNSISSTYLADLVEYVQDKEFSVNVISKSGTTTEPAI

AFRVFKELLVKKYGQEEANKRIYATTDKVKGAVKVEADANNWETFVVPDNVGGRFSVLTAVGLLPIAASGADITALM

EGANAARKDLSSDKISENIAYQYAAVRNVLYRKGYITEILANYEPSLQYFGEWWKQLAGESEGKDQKGIYPTSANFS

TDLHSLGQFIQEGYRNLEETVVRVEKPRKNVTIPELTEDLDGLGYLQGKDVDFVNKKATDGVLLAHTDGGVPNMFVT

LPTQDAYTLGYTIYFFELAIGLSGYLNSVNPFDQPGVEAYRNMEALLGKPGFEELSAELNARL

SeqID 244

MKEKQTAGRRQLEEFAPEFARYNDDILFGEVWAKEDHLTDKTRSIITISALISGGNLEQLEHHLQFAKQNGVTKEEI

ADIITHLAFYVGWPKAWSAFNKAKEIWI

SeqID 245

AWRALEDAQKSGKVKSIGVSNFLEKDLENILKNGHVKPAVNQILAHIGNTPFDLIDYCQSKGIQVEAYSPIAHGQAL

KSDGIQKMAEKYGVSVAQLCIQYLLQLNLIVLPKASSKEHLQSNLDFDFVISDEDMSILKSLMFDDYGEFSNFPVFS

EK

SeqID 246

MFRRSKNNSYDTSQTKQRFSIKKFKFGAASVLIGLSFLGGVTQGNLNIFEESIVAASTIPGSAATLNTSITKNIQNG

NAYIDLYDVKNGLIDPQNLIVLNPSSYSANYYIKQGAKYYSNPSEITTTGSATITFNILDETGNPHKKADGQIDIVS

VNLTIYDSTALRNRIDEVINNANDPKWSDGSRDEVLTGLEKIKKDIDNNPKTQIDIDNKINEVNEIEKLLVVSLPDK

IKYSPEAKHRTVEQHAELDAKDSIANTDELPSNSTYNWKNGHKPDTSTSGEKDGIVEVHYPDGTVDDVNVKVTVTSK

KTDNTAPTLTVTPEQQTVKVDEDITFTVTAEDENEVELGLDDLKAKYENDIIGARVKIKYLTKEPNKKVMEVTIMKA

TLADKGAITFTAKDKAGNQAEPKTVTINVLPPDKIKYSPEAKHRTVEQHAELDAKDSIANTDELPSNSTYNWKNGHK

PDTSTSGEKDGIVEVHYPDGTVDDVNVKVTVTSKKTDNTAPTLTVTPEQQTVKVDEDITFTVTAEDENEVELGLDDL

KAKYENDIIGARVKIKYLTKEPNKKVMEVTIMKATLADKGAITFTAKDKAGNQAEPKTVTINVLPPDKIKYSPEAKH

RTVEQHAELDAKDSIANTDELPSNSTYNWKNGHKPDTSTPGEKNAVVVVTYPDKSTDEVPVKVTVVDPRTDAEKNDP

AGKDQTVKVGEQPDPTKSLEAVPAGSTVAYKEPVDTKTPGEKNAIVVVTYPDKSTDEVPVKVTVVDPRTDAEKNDPA

GKDQTVKVGEQPDPTKSLEAVPAGSTVAYKEPVDTKTPGEKNAIVVVTYPDKSTDEVPVKVTVVDPRTDAEKNDPAG

KDQTVKVGEQPDPTKSLEAVPAGSTVAYKEPVDTKTPGEKNAVVVVTYPDKSTDEVPVKVTVVDPRTDAEKNDPAGK

DQTVKVGEQPDPTKSLEAVPAGSTVAYKEPVDTKTPGEKNAVVVVTYPDKSTDEVPVKVTVVDPRTDAEKNDPAGGE

TTVPQGTPISDEEITGLVKIPEGSNGVPKVVGDRPNTDVPGDYKVTVEVTYPDGTKDTVAVTVHVTPKPVPDKDKYD

PTGKSQQVNGKGNKLPATGESATPFFNVAALTIISSVGLLSVSKKKED

SeqID 247

MQILEDYDGRALPKLETDRLILRQRTVGDVPAMFDYVCLEEVAYPAGLSPIASLEDEYDYFENRYYQNLEKAKLPSG

YGITVKGSDRIIGSCAFNHRREDDVFEIGYLLHPDYWGHGYMTEAVAALIEVGFTLLNLHKIEIRCYDYNKQSQRVA

EKLGFTLEATIRDRKDNQGNRCVNLIYGLLRSEWE

SeqID 248

MSKELSPKYNPAEVEEGRYQTWLDQDVFKPSGDTEAKPYSIVIPPPNVTGKLHLGHAWDTTLQDIIIRQKRMQGFDT

LWLPGMDHAGIATQAKVEERLREQGISRYDLGREKFLDKVWEWKDEYAATIKSQWGKMGLSVDYSRERFTLDEGLSK

AVRKVFVDLYNKGWIYRGEFIINWDPAARTALSDIEVIHKDVEGAFYHMNYMLEDGSRALEVATTRPETMFGDVAVA

VNPEDPRYKDLIGQNVILPIINKPIPIIADEHADPEFGTGVVKITPAHDPNDFAVGQRHNLPQVNVMNDDGTMNELA

DEFNGMDRFEARKAVVKLESLGNLVKIEKMTTHSVGHSERTGVVVEPRLSTQWFVKMDQLAKNAIANQDTEDKVEFY

PPRFNDTFMSWMENVHDWVISRQLWWGHQIPAWYNVNGEMYVGEDAPEGDGWTQDEDVLDTWFSSALWPFSTMGWPD

TEAADFKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGRQPFSNVLIHGLIRDEEGRKMSKSLGNGIDPMDVIEKYG

ADALRWFLSNGSAPGQDVRFSYEKMDASWNFINKIWNISRYILMNNEGLTLDQARENVEKVVNSQVGNVTDRWILHN

LNETVGKVTESFDKFEFGVAGHILYNFIWEEFANWYVELTKEVLYSDNEDEKVVTRSVLLYTLDQILRLLHPIMPFV

TEEIFGQYAEGSIVLASYPQVNATFENQTAHKGVESLKDLIRSVRNSRAEVNVAPSKPITILVKTSDSELESFFKDN

SNYIKRFTNPETLEISSAITAPELAMTSIITGAEIFLPLADLLNVEEELARLEKELAKWQKELNMVGKKLSNERFVA

NAKPEVVQKEKDKQTDYQTYDDATIARIEEMKKLNND

SeqID 249

MVEPIISIQGLHKSFGKNEVLKGIDLDIHQGEVVVIIGPSGSGKSTFLRTMNLLEVPTKGTVTFEGIDITDKKNDIF

KMREKMGMVFQQFNLFPNMTVLENITLSPIKTKGLSKLDAQTKAYELLEKVGLKEKANAYPASLSGGQQQRIAIARG

LAMNPDVLLFDEPTSALDPEMVGEVLTVMQDLAKSGMTMVIVTHEMGFAREVADRVIFMDAGIIVEQGTPKEVFEQT

KEIRTRDFLSKVL

SeqID 250

MNYKEIYQEWLENDSLGRDIKSDLEAIKGDESEIQDRFYKTLEFGTAGLRGKLGAGTNRMNTYMVGKAAQALANTII

DHGPEALARGIAVSYDVRYQSKEFAELTCSIMAANGIKSYIYKGIRPTPMCSYAIRALGCVSGVMVTASHNPQAYNG

YKAYWKEGSQILDDIADQIANHMDAITDYQQIRQIPFEEALASGLASYIDESIEEAYKKEVLGLTINDTNIDKSVRV

VYTPLNGVGNLPVREVLRRRGFENVYVVPEQEMPDPDFTTVGYPNPEVPKAFAYSESLGKSVDADILLATDPDCDRV

ALEVKDSKGEYIFLNGNKIGALLSYYIFSQRCALGNLPHHPVLVKSIVTGDLSKVIADKYNIETVETLTGFKNICGK

ANEYDISKDKTYLFGYEESIGFCYGTFVRDKDAVSASMMVVEMTAYYKERGQTLLDVLQTIYDEFGYYNERQFSLEL

EGAEGQERISRIMEDFRQDPILQVGEMRLENSIDFKDGYKDFPKQNCLKYYFNEGSWYALRPSGTEPKIKCYLYTIG

CTEADSLSKLNAIESACRAKMNSTK

SeqID 251

MYREITAVEHDRFVSESNQTNLLQSSNWPKVKDNWGSQLLGFFDGETQIASASILIKSLPLGFSMLYIPRGPIMDYS

NLDIVTKVLKDLKAFGKKQRALFIKCDPLIYLKMVNAKDFENSPDEKEGLIAIDHLQRAGADWTGRTTDLAHTIQPR

FQANLYANQFGLDKMSKKTRQAIRTSKNKGVDIQFGSHELLEDFAELMKKTEDRKGINLRGIDYYQKLLDTYPNNSY

ITMASLDVAKRLEKIEKECQIAQSERIKSLELNREKKVKQHQGTIDRLNKEIDFLKEAQKAYDRDIIPLAATLTLEF

GNTSENIYAGMDDYFKSYSAPIYTWFETAQRAFERGNIWQNMGGIENDLSGGLYHFKSKFEPIIEEFIGEFNIPVNR

LLYKASNYVYALRKKRNS

SeqID 252

MACTTILVGKKASYDGSTMIARTEDSVNGDFTPKKLKVMTSKDQPRHYKSVLSNFEVDLPDNPLPYTSVPDALGKDG

IWGEAGINSKNVAMSATETITTNSRVLGADPLVSDGIGEEDILTLVLPYIQSAREGVERLGAILEKYGTYESNGIAF

SDTEEIWWLETIGGHHWIARRVPDDVYVTNPNQLGIDHFEFNNCDDYMCSSDLKEFIEQYHLDLTYSNEHFNPRYAF

GSQRDKDRHYNTPRSWAMQRFLNPEIEQDPRSLFIPWCQKPYRKITVEDIKYVLSDHYQDSVYDPYGPEGDAVSRRA

FRSVGINRTSQTSILQLRPNKSLETTGVQWLSYGSMPFATMVPLFTQVETVPNYFSNTTKDASTDNFYWTNRLIAAL

ADPHFYQHEADIESYIERTMAQGHAHINGVDREVAENKEIDFQQKNQEMSDYIQKESQELLNRILFDASNLMTNRFS

MGD

SeqID 253

MRKKFLLLMSFVAMFAAWQLVQVKQVWADSKLKVVTTFYPVYEFTKNVVGDKADVSMLIKAGTEPHDFEPSTKNIAA

IQDSNAFVYMDDNMETWAPKVAKSVKSKKVTTIKGTGDMLLTKGVEEEGEEHEGHGHEGHHHELDPHVWLSPERAIS

VVENIRNKFVKAYPKDAASFNKNADAYIAKLKELDKEYKNGLSNAKQKSFVTQHAAFGYMALDYGLNQVPIAGLTPD

AEPSSKRLGELAKYIKKYNINYIYFEENASNKVAKTLADEVGVKTAVLSPLEGLSKKEMAAGEDYFSVMRRNLKVLK

KTTDVAGKEVAPEEDKTKTVETGYFKTKDVKDRKLTDYSGNWQSVYPLLQDGTLDPVWDYKAKSKKDMTAAEYKKYY

TAGYKTDVESIKIDGKKHQMTFVRNGKSQTFTYKYAGYKILTYKKGNRGVRYLFEAKEKDAGQFKYIQFSDHGIKPN

KAEHFHIFWGSESQEKLFEEMEWPTYFPAKMSGREVAQDLMSH

SeqID 254

MKLSKKLLFSAAVLTMVAGSTVEPVAQFATGMSIVRAAEVSQERPAKTTVNIYKLQADSYKSEITSNGGIENKDGEV

ISNYAKLGDNVKGLQGVQFKRYKVKTDISVDELKKLTTVEAADAKVGTILEEGVSLPQKTNAQGLVVDALDSKSNVR

YLYVEDLKNSPSNITKAYAVPFVLELPVANSTGTGFLSEINIYPKNVVTDEPKTDKDVKKLGQDDAGYTIGEEFKWF

LKSTIPANLGDYEKFEITDKFADGLTYKSVGKIKIGSKTLNRDEHYTIDEPTVDNQNTLKITFKPEKFKEIAELLKG

MTLVKNQDALDKATANTDDAAFLEIPVASTINEKAVLGKAIENTFELQYDHTPDKADNPKPSNPPRKPEVHTGGKRF

VKKDSTETQTLGGAEFDLLASDGTAVKWTDALIKANTNKNYIAGEAVTGQPIKLKSHTDGTFEIKGLAYAVDANAEG

TAVTYKLKETKAPEGYVIPDKEIEFTVSQTSYNTKPTDITVDSADATPDTIKNNKRPSIPNTGGIGTAIFVAIGAAV

MAFAVKGMKRRTKDN

SeqID 255

MKKRQKIWRGLSVTLLILSQIPFGILVQGETQDTNQALGKVIVKKTGDNATPLGKATFVLKNDNDKSETSHETVEGS

GEATFENIKPGDYTLREETAPIGYKKTDKTDKVKVADNGATIIEGMDADKAEKRKEVLNAQYPKSAIYEDTKENYPL

VNVEGSKVGEQYKALNPINGKDGRREIAEGWLSKKITGVNDLDKNKYKIELTVEGKTTVETKELNQPLDVVVLLDNS

NSMNNERANNSQRALKAGEAVEKLIDKITSNKDNRVALVTYASTIFDGTEATVSKGVADQNGKALNDSVSWDYHKTT

FTATTHNYSYLNLTNDANEVNILKSRIPKEAEHINGDRTLYQFGATFTQKALMKANEILETQSSNARKKLIFHVTDG

VPTMSYAINFNPYISTSYQNQFNSFLNKIPDRSGILQEDFIINGDDYQIVKGDGESFKLFSDRKVPVTGGTTQAAYR

VPQNQLSVMSNEGYAINSGYIYLYWRDYNWVYPFDPKTKKVSATKQIKTHGEPTTLYFNGNIRPKGYDIFTVGIGVN

GDPGATPLEAEKFMQSISSKTENYTNVDDTNKIYDELNKYFKTIVEEKHSIVDGNVTDPMGEMIEFQLKNGQSFTHD

DYVLVGNDGSQLKNGVALGGPNSDGGILKDVTVTYDKTSQTIKINHLNLGSGQKVVLTYDVRLKDNYISNKFYNTNN

RTTLSPKSEKEPNTIRDFPIPKIRDVREFPVLTISNQKKMGEVEFIKVNKDKHSESLLGAKFQLQIEKDFSGYKQFV

PEGSDVTTKNDGKIYFKALQDGNYKLYEISSPDGYIEVKTKPVVTFTIQNGEVTNLKADPNANKNQIGYLEGNGKHL

ITNTPKRPPGVFPKTGGIGTIVYILVGSTFMILTICSFRRKQL

SeqID 256

GAKKAGWTEYARMLEVREQVDHVMIPKINQDLPIYAGPEEDNLQRGVGHLEGISLPIGGASTHAVLSGQRGMPAARL

FADLDKMKKGDYFYVTNLKETLAYQVDRIMVIEPSQLDAVSIEEDKDYVTLLTCTPYMGSLSTVMGDLSLTTRENQL

GSLSFWMFKAMRILLLKFLKLRKPKACRLMSLISLLVASNLALERDS

SeqID 257

MSDVVEKQTAKSFIMNVLNGLALGTVIVLIPGAILGELMKALLPMWSGFATLIAATAVATSMMGLVIGIMVGLNFKF

NPIQSASLGLAVMFAGGAATFLKGAIMLKGTGDIINMGITAALGVLLIQFLSDKTKSFTLIVIPTVTLLLVGGVGHV

LLPYVKMITTMIGQGIASLLGLQPVLMSILIAMIFCFLIVSPITTVGIALAISLSGIGSGAANLGICAASFGLCMAG

WSVNSKGTALAHVLGSPKISMANVLAKPKIMLPMISSAAILGILGALFNIQGTPASAGFGISGLIGPINALNLAKGG

WSVMNMLLIIIIFVAAPIILNFIFNYLFIKVLKIIDPMDYKLDI

SeqID 258

MARPLVEQTADRLLHLILEREYPVGAKLPNEYELAEDLDVGRSTIREAVRSLATRNILEVRQGSGTYISSKKGVSED

PLGFSLIKDTDRLTSDLFELRLLLEPRIAELVAYRITDDQLQLLEKLVGDIEDAVHAGDPKHLLLDVEFHSMLAKYS

GNIAMDSLLPVINQSIHLINANYTNRQMKSDSLEAHREIIKAIREKNPVAAHDAMLMHIMSVRRSALKA

SeqID 259

MIKKNKVFLGVLLVLVVILGGGVLFYQSQFQKTTNQALAIAYKDAKVAKKDVIHQKIDKEFENFRGSYEIEFNTKSA

EYSYHVDAKTGQILERDMDNNGFSKSTSQSSSSSSQKSHKISQEEAKKIAFKDANIEESEVSNLKIKEEIENGKSVY

DIDFVDLKNKNEVDYQIDAETGKIIERSRDHMND

SeqID 260

LNRKKRYRLTVKKQNASIPRRLNLLFFIIVLLFTVLILRLEQMQIGQQSFYMKKLTALTSYTVKESKARGQIFDAKG

VVLVENDERPTVAFSRGNNISSQSIKELANKLSHYITLTEVASSDRAKRDYYLADKANYKKVVESLPDSKRYDKFGN

HLAESTVYANAVAAVPVSAINYSEDELKVVALFNQMNATPTFGSVKLSTGELSDDQIKKLDADKKELLGISVTSNWH

RRKKGTSLSDILGTISTEKAGLPREEVKKYLKKGYSLNDRVGTSYLEKQYEDDLQGIRQIRKVVVNKKGKVVSDNIT

QEGKSGRNLKLTIDLNYQNKVESILKQYYGSELSSGRASFSEGMYAVAIEPSTGKVLAMAGLKNDHGNLVDDSLGTI

AKNFTPGSVVKGATLSSGWENKVLRGNEVLYDQEIANIRSWFTRGLTPISAAQALEYSSNTYMVQVALRLMGQDYNT

GDALTDRGYQEAMAKLRKTYGEYGLGVSTGLDLPESEGYVPGKYSLGTTLMESFGQYDAYTPMQLGQYISTIANNGN

RLAPHVVSDIYEGNDSNKFAQLVRSITPKTLNKIAISDQELAIIQEGFYNVVNSGSGYATGTSMRGNVTTISGKTGT

AETFAAKNVNGQTVSTYNLNAIAYDTNRKIAVAVMYPHVTTDTKSHQLVARDMIDQYISQFTGQ

SeqID 261

MTVFPKHFLWGGAVAANQVEGAFRTDGKGLSVQDVLPNGGLGDFTAKPTPDNLKLEAIDFYHNYKNDIKLFAEMGFK

VFRTSIAWSRIFPNGDDSAPNEAGLQFYDNLFDELLKYNIEPLVTLSHYETPLHLAKTYNGWADRRLIAFFEKFAQT

VMERYKDKVKYWLTFNEVNSILHMPFTSGAIMTDKSQLSPQELYQAIHHELVASARVTKLGRSINPNFKIGCMILAM

PAYPMTSDPRDVLAARQFEQHNLLFSDIHVRGKYPTYIQSYFKNNGIKIKFEEGDEEVLAQNTVDFLSFSYYMSVTQ

AYDFENYQSGQGNILGGLTNPHLTTSEWGWQIDPIGLRLVLNQYYERYQIPLFIVENGLGAKDQLIETLDGDYTVED

DYRIDYMNQHLVQVAKAIEDGVEIMGYTSWGCIDCVSMSTAQLSKRYGLIYVDRNDDGTGSLQRYKKKSFGWYQKVI

KTNGQSLFEHHNR

SeqID 262

MATFQIKEKNFSLGGKFTITDQTGLPCYHVEGSLFPLPKTFKVFDEEGHLISQIEKKVLSFLPKFNVTLANGNHFTI

KKDFSFLKPHYTIEDLDMEVKGNFWDIDFQLLKDNQVIANISQQWFRMTSTYQVEVYNETYNDLTISLVIAIDYVKE

LEKNASN

SeqID 263

MKKITTLILASSLLLVATTSVKADDNFEMPTRYVKMSEKSKAFYQRLQEKQRKAHTTVKTFNNSEIRHQLPLKQEKA

RNDIYNLGILISQESKGFIQRIDNAYSLENVSDIVNEAQALYKRNYDLFEKIKSTRDKVQVLLASHQDNTDLKNFYA

ELDDMYEHVYLNESRVEAINRNIQKYN

SeqID 264

MKLKKFFEDLLAKLEYRPIQVFMRHFQSAEMDLSAIAVAYYLLVTAFPLLVIAANIFPYFHINVSDLLSLMQKNLPK

NIYEPASRLAVDAFSKPSTGILGFASLTAFWTMSKSLTSLQKAINKAYGVDQHRDFVISRLVGVGTGLIILFLLTFV

LIFSTFSKPVLQIIVNMYDLGDTLTAWLLNLAQPVTFLTIFLGIGILYFILPNARIRKVRYVIPGTLFSTFVIGFFS

NLISQYVLNRVEKMVDIKTFGSVVIFILMLWFIFLAHIMILGAILNASVQEIATGKIESRRGDIMSLIQKSKEEK

SeqID 265

MKLLYTDINHDMTEILVNQAAHAAEAGWRIFYIAPNSLSFEKERAVLENLPQEASFAITITRFAQLARYFTLNQPNQ

KESLNDIGLAMIFYRALASFEDGQLKVFGRLKQDASFISQLVDLYKELQTANLSILDLKYLHSPEKFEDLLAIFLVV

SDLLREGEYDNQSKIAFFTEQVRSGQLDVDLKNTILIVDGFTRFSAEEEALIKSLSSRCQEIIIGAYASQKAYKANF

TNGNIYSAGVDFLRYLATTFQTKPEFILSKWESKSGFEMISKNIEGKHDFTNSSHILDDTAKDCITIWECINQKDEV

EHVARAIRQKLYQGYRYKDILVLLGDVDSYKLQLSKIFEQYDIPYYFGKAETMAAHPLVHFMDSLSRIKRYRFRAED

VLNLFKTGIYGEISQDDLDYFEAYISYADIKGPKKFFTDFVVGAKKFDLGRLNTIRQSLLAPLESFVKTKKQDGIKT

LNQFMFFLTQVGLSDNLSRLVGQMSENEQEKHQEVWKTFTDILEQFQTIFGQEKLNLDEFLSLLNSGMMQAEYRMVP

AYVDVVTVKSYDLVEPHSNQFVYALGMTQSHFPKIAQNKSLISDIERQLINDANDTDGHFDIMTRENLKKNHFAALS

LFNAAKQALVLTIPQLLNESEDQMSPYLIELELRDIGVPFNHKGRQSLKEEADNIGNYKALLSRVVDLYRSAIDKTK

EEQTFWSVAVRYLRRQLTSKGIEIPIITDSLDTVTVSSDVMTRRFPEDDPLKLSSSALTTFYNNQYKYFLQYVLGLE

EQDSIHPDMRHHGTYLHRVFEILMKNQGIESFEEKLNSAINKTNQEDVFKSLYSEDAESRYSLEILEDIARATATIL

RQDSQMTVESEEERFELMIDNTIKINGIIDRIDRLSDGSLGVVDYKSSAQKFDIQKFYNGLSPQLVTYIDAISRDKE

VEQKPPIFGAMYLHMQEPKQDLSKIKNLDDLVTKNHQALTYKGLFSEAEKEFLANGKYHLKDSLYSEAEIAILQAHN

QLLYKKASETIKSGKFLINPYTEDAKTVDGDQFKSITGFEADRHMARARALYKLPAKEKRQGFLTLMQQEEENDDL

SeqID 266

MSETKVMALREAINVAMSEEMRKDEKVFLMGEDVGVYGGDFGTSVGMLEEFGAKRVRDTPISEAAIAGSAIGAAQTG

LRPIVDLTFMDFVTIAMDAIVNQGAKTNYMFGGGLSTPVTFRVASGSGIGSAAQHSQSLEAWLTHIPGLKVVAPGTV

NESKALLKSSILDNNPVIFLEPKALYGKKEEVNMDPDFYIPLGKGDIKREGTDLTIVSYGRMLERVMQAAEEVAEEG

INVEVVDPRTLIPLDKELIIDSVKKTGKLILVNDAYKTGGFTGEIATMVAESEAFDYLDHPIVRLASEDVPVPYSRV

LEQGILPDVAKIKDAIYKVVNKGK

SeqID 267

MAFDVIMPKLGVDMQEGEILEWKKNEGDTVNEGDVLLEIMSDKTNMEIEAEDTGVLLKIVHQAGDVVPVTEVIAYIG

EEGEEVGTSSPSADATITAEDGQSVSGPAAPSQETVAAATPKEELAADEYDIVVVGGGPAGYYAAIRGAQLGGKIAI

VEKTEFGGTCLNVGCIPTKTYLKNAEILDGLKVAAGRGINLASTNYAIDMDKTVAFKNSVVKTLTGGVRGLLKANKV

EIFNGLGQVNPDKSVVIGDKVIKGRNVVLATGSKVSRINIPGIESPLVLTSDDILDLREIPKSLAVMGGGVVGIELG

LQWASYGVDVTVIEMADRIIPAMDKEVSLELQKILAKKGMKIKTSVGVSEIVEANNQLTLKLNNGEEVVADKALLSI

GRVPQMNGLENLEPELEMERGRIKVNAYQETSIPGIYAPGDVNGTRMLAHAAYRMGEVAAENALGGNKRKAHLDFTP

AAVYTHPEVAMVGMTEEQAREQYGDILVGKNSFTGNGRAIASNEAHGFVKVIAEPKYKEILGVHIIGPAAAELINEA

STIMENELTVYDVAQSIHGHPTFSEVMYEAFLDVLGEAIHNPPKRK

SeqID 268

MGKYFGTDGVRGEANVELTPELAFKLGRFGGYVLSQHETDRPRVFVARDTRISGEMLESALIAGLLSVGIEVYKLGV

LATPGVSYLVRTEKASAGVMISASHNPALDNGIKFFGSDGFKLDDDRELFIEALLDAKEDTLPRPSAQGLGTLVDYP

EGLRKYEKFMESTGIDLEGMKVALDTANGAATASARNIFLDLNADISVIGDQPDGLNINDGVGSTHPEQLQSLVREN

GSDIGLAFDGDSDRLIAVDENGEIVDGDKIMFIIGKYLSDKGQLAQNTIVTTVMSNLGFHKALDREGIHKAITAVGD

RYVVEEMRKSGYNLGGEQSGHVIIMDYNTTGDGQLTAIQLTKVMKETGKKLSELASEVTIYPQKLVNIRVENNMKDK

AMEVPAIAEIIAKMEEEMDGNGRILVRPSGTEPLLRVMAEAPTNEAVDYYVDTIADVVRTEIGLD

SeqID 269

MKGQKIIALAGLVLSCHFALTACHTQEHKNSHHIKTKQVAKKKANKKKVSVKESHKKRKGVAGVDFPTDDGFLLTKD

SKILSHPDSGIVVAHGNHSHFIFYSDLKGSKFSYIPNANAKTNKKQAVRNFKAGAVAVNTLNDDGYVFNPADIVSED

ANGYVVRHGDHFHYIPKASLSQQKQVQASRAVSRLGNQNNSHYRVNSSKIAGLHHPTSDGFLFNGQGIKGTTPTGIL

VEHHNHLHFISFADLRKGGWGSIADRYQPQKKVDSKKQSPSSKKPRTENTLPKDIKDKLAYLARELHLDISRIRVLK

TLNGEIGFEYPHDDHTHVIMAKDIDLSKPIPNPHHDDEDHHKGHHHDESDHKHEEHEHTKSNKLSDEDQKKLIYLAE

KLGLNPNQIEVLTSEDGSIIFKYPHDDHSHTIASKDIEIGKPIPDGHHDHSHAKDKVGMATLKQIGFDDEIIQDILH

ADAPTPFPSNETNPEKMRQWLATVTKINIGQRTNPFQRFGLSLMPNIEVLGIGFTPINDMTPVLQFKKLKQLWMTNT

GITDYSFLDKMPLLEGLDISQNGIKDLSFLTKYKQLSLIAAANNGITSLKPLAELPNLQFLVLSHNNISDLTPLSNL

TKLQELHLDHNNVKNLSALSGKKDLKVLDLSNNKSADLSTLKTTSLETLLLNETNTSNLSFLKQNPKVSNLTINNAK

LASLDGIEESDEIVKVEAEGNQIKSLVLKNKQGSLKFLNVTNNQLTSLEGVNNYTSLETLSVSKNKLESLDIKTPNK

TVTNLDFSHNNVPTSQLKLNEKNIPEAVAKNFPAVVEGSMVGNGSLAEKAAMASKEDKQVSDNTNHQKNTEKSAQAN

ADSKKENPKTHDEHHDHEETDHAHVGHHHH

SeqID 270

MNKRVKIVATLGPAVEFRGGKKFGESGYWGESLDVEASAEKIAQLIKEGANVFRFNFSHGDHAEQGARMATVRKAEE

IAGQKVGFLLDTKGPEIRTELFEDGSDFHSYTTGTKLRVATKQGIKSTPEVIALNVAGGLDIFDDVEVGKQILVDDG

KLGLTVFAKDKDTREFEVVVENDGLIGKQKGVNIPYTKIPFPALAERDNADIRFGLEQGLNFIAISFVRTAKDVNEV

RAICEETGNGHVKLFAKAENQQGIDNIDEIIEAADGIMIARGDMGIEVPFEMVPVYQKMIITKVNAAGKAVITATNM

LETMTDKPRATRSEVSDVFNAVIDGTDATMLSGESANGKYPVESVRTMATIDKNAQTLLNEYGRLDSSAFRRNNKTD

VIASAVKDATHSMDIKLVVTITETGNTARAISKFRPDADILAVTFDEKVQRSLMINWGVIPVLADKPASTDDMFEVA

ERVALEAGLVESGDNIVIVAGVPVGTGGTNTMVRTVK

SeqID 271

MTATKQHKKVILVGDGAVGSSYAFALVNQGIAQELGIIEIPALFDKAVGDAEDLSHALAFTSPKKIYAATYADCADA

DLVVITAGAPQKPGETRLDLVGKNLAINKSIVTQVVESGFNGIFLVAANPVDVLTYSTWKFSGFPKERVIGSGTSLD

SARFRQALADKIGVDARSVHAYIMGEHGDSEFAVWSHANVAGVQLEQWLQENRDIDEQGLVDLFISVRDAAYSIINK

KGATYYGIAVALARITKAILDDENAVLPLSVYQEGQYGDVKDVFIGQPAIVGAHGIVRPVNIPLNDAELQKMQASAE

QLKDIIDEAWKNPEFQEASKN

SeqID 272

MQDKNLVDVNLTSEMKTSFIDYAMSVIVARALPDVRDGLKPVHRRILYGMNELGVTPDKPHKKSARITGDVMGKYHP

HGDSSIYEAMVRMAQWWSYRHMLVDGHGNFGSMDGDGAAAQRYTEARMSKIALEMLRDINKNTVDFQDNYDGSEREP

LVLPARFPNLLVNGATGIAVGMATNIPPHNLGESIDAVKLVMDNPDVTTRELMEVIPGPDFPTGALVMGRSGIHRAY

ETGKGSIVLRSRTEIETTSNGKERIVVTEFPYGVNKTKVHEHIVRLAQEKRIEGITAVRDESSREGVRFVIEVRRDA

SANVILNNLFKLTSLQTNFSFNMLAIEKGVPKILSLRQIIDNYIEHQKEVIVRRTQFDKAKAEARAHILEGLLVALD

HLDEVITIIRNSETDTIAQAELMSRFELSERQSQAILDMRLRRLTGLERDKIQSEYNDLLALIADLADILAKPERVV

TIIKEEMDEVKRKYADARRTELMIGEVLSLEDEDLIEEEDVLITLSNKGYIKRLAQDEFRAQKRGGRGIQGTGVNND

DFVRELVSTSTHDTVLFFTNLGRVYRLKAYEIPEYGRTAKGLPIVNLLKLDEGETIQTIINARKEDVANKYFFFTTQ

QGIVKRTSVSEFSNIRQNGLRAINLKENDELINVLLIDENEDVIIGTRTGYSVRFKVNAVRNMGRTATGVRGVNLRE

GDKVVGASRIVNGQEVLIITEKGYGKRTEASEYPTKGRGGKGIKTANITAKNGPLARLVTINGNEDIMVITDTGVII

RTNVANISQTGRSTMGVKVMRLDQEAKIVTVALVEQEIEDKSNIEDTKE

SeqID 273

MTIFDERELKERFTHENRVSFYEFVAKYDAQMVPVMKAKGYRCIHSMERTVVFTFGEFTIRRRRWQKGEHWVVPVDE

KLGLKKNVRYSLEFMYQIASLATMMPYEKVIKVVQMMYCIVITKPTVVKAVKISRELLKEKEAYRFFDEDIPVDKEP

VDMIYLEGDGVMVKAREEGLDNRNVDLSHFVVHTGSQKVGSNRFVLQNKKEFVSLDNRQTRQKILDYLYNHFYIAPN

TLLITNSDGGHGYTPYVFKEIAKALKVKQHEHFWDRYHVNEKIKSFFKLYPVELMTGAFQSIKQHDKEKLRTVLDTT

EALILMEEEMEGFNQFKRKLLNNFQYTKSAELRGFSRAGIGVMESQHRKITYRMKKRGMYWTIQGAETMSQLIVLSY

EGQLRDLFFGSWREDYQKYQELENLSAGKIKHEQNKINKRYDLQTLGRLRYGRHRNL

SeqID 274

MAYLSKLSDLDPSLMDADSEQIYIPKVLFEHNDFKGLTYKEILLYSFLLNRLREPLDFIQKGYDDNEDTYVHFKVED

LCELLNQSKTTVISLKKRLAQYGLIEEVKAGSHQPNRIYLTDKLVPYIKG

SeqID 275

MTDNRFAQLKENFEKGSPKRRVPTSRPIAAQKAPESYNKKGRYPFSLHQDVRYDKLEALVAYHGAKSASDYLERLIV

QEWEKMQRKLKNKEK

SeqID 276

MFSWLEALYYTLIQLAKVNRLNALFLVSVVGYLCYQGIKLVRKTIRNFFQLMKGFIGDRENIKKCIKNKKEALVHSW

KHRQDIDWKSTGKDKSKQLWNLMKRLATVAPSFLFLLLGNVLFRLIYQLPFVKQDRKRFDKEMKPLLTFKNYRSFVF

MGIGFSFIAFILTNYFVTVLRAAIRFLYFSIMTLRDNSQVVSFNVDSLLIQNLFNARVFVIAPILAVPIFLIGLVVA

WRSAWVNFEQYRDYNHNEEGDDRFATVKEIHQQYKKVPNLTETYPGEGGVPVLHETRKNLTGLTLKSQMLWQNRTFS

RYLTNAERILGLLSTPSGDYYIDDSTTNLITMGITRSGKGEAHIAPIIDINSRAEIQPSLIIADPKGEHYQSSYKTM

RRRGYDVNVLSFQNMDWSMSYNPLALAIAAAKKGYYEMTQTRVNAVAEAIYRKTKPGSGNGNAKYWEDTSISLFNAI

AMALMDRANETVRNGETDAWDTVTVRNIAKFLTDLGSEEVFVNDFGEIVENPDKNQQVKKKSKITVYFDNLRKINQE

QFSKFRDMADLNFRSSDFASEETKGNVFSSMMSGINLFLQDNIAKLTSKNSIDLESVGFPRRLSIKFRSSSNVAMRN

EYTHKTAKVTITSQAVWGKTTKQVIHVDAATALIDGEGYLTYVIEPQKPDQFLVTIDFNHENNGGSAIRHKTFQFSA

EKVYKKRGNVITLDDYTKKPVLDHIKVTVLNKQDDNLLQKEDIDLIYSDNPKVIYLVTPPNRTEYNSIVSLFLDQLF

NANYELALSNGRKCVNRILHILDEFTNIPAIPHMDTKISIGLGQNILYYLWIQNLKQLVSEYGENTAETIRENCSLK

VYIKSTAPATNEYFSKELGTRTITRRRRSSNILDEANPNVSIENPRQELLTPTQLSKLQEGEAVILRGVKGRDNAGR

KITTDPIFLHEKTSLPYRYMFLQEEFDQSMALADIPVESGHRDLDLQDIAVGAQSTFNKIIDWRMALTDRMRTNGKI

PQLASRKQTIKALSQSQFTSPADLTQAVIAEVFDEEDDDDLFFVDDVM

SeqID 277

MNSNTKGHGFFRKSKAYGLVCAIALAGAFTLATSQVSADQVTTQATTQTVTQNQAETVTSTQLDKAVATAKKAAVAV

TTTPAVNHATTTDAQADLANQTQAVKDVTAKAQANTQAIKDATAENAKIDAENKAEAERVAKENKEGQAAVDARNKA

GQAAVDARNKAKQQAQDDQKAKIDAENKAESQRVSQLNAQNAKKIDAENKDAQAKANATNAQLQKDYQAKLAEIKSV

EAYNAGVRQRNKDAQAKADATNAQLQKDYQAKLALYNQALKAKAEADKQSINNVAFDIKAQAKGVDNAEYGNSIMTA

KTKPDGSFEFNHDMIDGVKTIGYGKLTGKVNHHYVANKDGSVTAFVDSVTLYKYEYRNVAQNAAVNQNIVFRVLTKD

GRPIFEKAHNGNKTFAETLNKTLQLNLKYELKPHASSGNVEVFKIHDDWVHDTHGSALVSYVNNNDAVPNVVIPERP

TPPKPVKVTPEAEKPVPEKPVEPKLVTPTLKTYTPVKFIPREYKPEPITPETFTPEKFTPAQPKVKPHVSIPEKINY

SVSVHPVLVPAANPSKAVIDEAGQSVNGKTVLPNATLDYVAKQNFSQYKGIKASAEAIAKGFAFVDQPNEALAELTV

KSIKASNGDDVSSLLEMRHVLSKDTLDQKLQSLIKEAGISPVGEFYMWTAKDPQAFYKAYVQKGLDITYNLSFKIKA

NFTKGQIKNGVAQIDFGNGYTGNIVVNDVTVPEVHKDILDKEDGKSINNSTVKLGDEVTYKLEGWVVPANRGYDLFE

YKFVDQLQHTHDLYLRDKVVAKVDVTLKDGTVIKKGTNLGEYTETVYNKTTGHYELAFKKEFLAKVSRESEFGADDF

IVVKRIKAGDVYNTADLYVNGYKVKSEAVVTHTTEKSKPVEPQKATPKAPAKGLPSTGEASMTPLTAIGAIILSALG

LAGFKKRQK

SeqID 278

MKQIKIITGLTVATLSAVVGNVYAEDITPTAPVNEPQVSSETAKTPQVTESQVNSAKVTADQAISDVNNQQIVVDEA

QKQKDQSQQNLVKATSTVTEAEKVAAEATPEVVKEAIKAVTEAKEAVTDAEANVVDAQKTEQKANQEVQSQAKTVDE

NVKVVADKESEVKQAEGVVTTAQEAIDSKTANTNASEAEKAVTEKQTKLETAETNLTEAQKQDAKIAEEKRLAEQEV

VNKQLAVTDTQTLLKKLVTEINNEKVSTSLENQAYFNQRDGSWAGYYGNYTFAATGCVPSSLAMVFTELARRQITPT

EIANYLWNNSNEFNKNYGGTSGKGLVQATKHFGFVPTHLASQSAIVEALQAGHHVLAAVQQDKFSPWGINYSHRIVL

RGYSNGNTYVYDPYNRANIGWYPVANLWNEQSRDAIDTSSVGVPFFKITTQKMAQLEAQKAQVQSSLNTAKNQLAKT

QDVLRTLEATPLKTPEAQAKLNQAKEALALAQADYTKAQEAVKLASQDLAVKEETLKNAQADLLTKQTALKDAQTVL

VASQVKLADLKATLATVENNVKKAQATLTDAKAIVGQKQAKLLALQNAPKILADAQAKLVTAKNDLANKMAILDEAV

AKLKSLQAVQAEAQKQYHVVFEAYKAVRDAKEQAKLAESYNHIIARGGEAIPVVDETDKITGYVDGSQKAVANEVTL

ALTSNGAPLESPVNKENQNVTKSSQALPHTGEAGLSLLSVLGVGLISTLGLTSLKKRRPH

SeqID 279

VRLTHVSLWMPSKRSWPLKLQLLRKGVIELEDELKTPYLDQYTDNLTAKVTKKSDDYQVYGRNKEVQSVIISLLRRT

KNNPILVGEAGVGKSAIVEGITLAILRGQVPEPLKGLTVRSLELSSLMSEDDEGFIAKFKKIIEEMVATRGHNLLFV

DEFHTIIGAGSQNGQALDAGNVIKPVLARGDIQLIGATTLDEFHEYIETDRALERRMQPVMVEEPTISQAITIIEQA

KVIYEKFHGIQISSDAVHQAIRLSVRYLTDRFLPDKAFDLIDEAATIASVEGKSKVTEKDIAQVLKDKTGIPVTTIL

KGDQERLEGFKERLMNRVKGQEDAIEAVVDAVTIAQAGLQNEKRPLASFLFLGPTGVGKTELAKAIAEALFDDEAAM

IRFDMSEYKQKEDVTKLIGNRATRIKGQLTEGVKQKPYCVLLLDEIEKAHSEVMDLFLQVLDDGRLTDSSGRLISFK

NTIVIMTTNIGAKKIINKWELKGNFKDLTDRDRKQFEKSMDSELQNEFRPEFLNRIENKLIFNLLERDVIEKIAEKN

LSEIADRMKRQNLTLSYEPSLIQYLSDVGTDVKNGARPLERLMKRKVLAPISVKSLQLDKSKQGYNVHLWVEGRAPD

GNHRQEQRQIHMEIEGERDNFFS

SeQID 280

MAEENAQQPSLRGKSRRERVEFARSRDILDVAHELNMELFRDGKNYRWKEHDSMVITPATNQWYWFSQRQGGDVIAL

VETIKEIGFNQAFEYLNEGTFKEFTVVNQVKEPFSYYLEPYEQLFVEARRYLKENRGLSDDTIDFFYDKGVLAQANA

KVGDMIEPVLVFKNLDKNGQVVGAALQGLVAAPDKYFGRGYLKQIMKNSQPYNGMHVDIGTPNRLVFAESSIDLMSY

YEIHKDSLSDVRLVSLEGLKTGTIGRHLIQLRAEMERRPLSSSWTDEILAQGLDEAVKQGYFKDGKNSHLLTLAVDN

DVKGKQLIEELKDKSIPVIDATPPKAEGQSKMDWNAYLQETKATFSTEKYQEKIDHLISDVILGDETYYLWHDDELV

NLGAGDSIIQAFHHQLEDRRYVINQAELYVEESSNDGATGYLSIEGHVLDKDGISDYLSDQALTDAEKVAFLETLQT

ELPDIWDEIVNHYDKVFEEVVVKYGLREKHADIIQEQELDLDPLVVPEAKEKSLEMNQETNTGGELFNRNSSFLGED

SPGTAPQPVEPTAQPDFPTNVRLHFTTDDGNMSNKAFRKNMRTLNLYANTMRDSAQWYLSEIADTTMSYVYKTPHEE

GVQVLSVHFGKKNWMHLTGVTPVYENWVDSLSEQFIDDIANSKGHFKNLKFALGTPDKLKVLNLLPEIIESDTFVFN

DLSSVQKLNNLDLSQALNPEDSDLLLLFRDEGLHQVPASLMRIKGDLEERLSHIDSGTVLGVYRERNGQLEQVSVNE

EYVKDSGQEMLSILQNKHYEEALDSGQEMVQTDGFSAEDFTKVLDAVYHVGVPDDLARVPEGVLPVWQKYLEVSEEN

QWDLEQMIDYADKNSLLVKDSAFYKEWKEDMIYKNDYHVRLQFAENWDNGVELPFRTEQLIDYKTFVTGLYEANQAH

HQRRQESQLPYTKTEFDIYAPGGQLIKDNVHYAIGDETRPVSQLMGLGYRRLPGYQELAVIDNSILSQLENKELNQE

IASEANEHSLNSQEIPKEDNYPREAFTSPKQDIKKGLAQRVEEIVAEDATKILVSSIPQVQENLSVEGNLVGTPQAD

NRMLYTNLEDFGQDYQLELAVYSPKRVDFLEDVQAPWTLALIRKEKKIGYLAYGSDWAKEFQIEEELEHLAAQIGDE

KVPEGLYKQAEVEAFIASHQGNESLQEPIPTIVAEPFDYTSASAYEISEHAFQKIREYTQSPEDLLEYMDFMSKFPQ

LSPRNVALIHEQWRGANAVATYEQWKAMGEALGIKPDDVVQTKATYVNKRTGETKEVVHQGLSVKTGEKSKITLFRP

LMVKMIPVLDENGQQLKNDKGNPKYKKLSEASLQEKALVKDGKLPVRQFQERDSKTGQPRFTTYKVFELSQTTLKPG

SYPKAMPNRHFNFNVDKVKTKEVLEGLCDYAEKIGVSLMKDDAHVLDNAKGAFYSEEQLILINPNNTPGEKIATTIH

ELAHATLHNPKLEKQYKELPKGQKEFEAEMTSYLLSKHFGLDTSEKAIHYMASWTDNLKALEDKQLADSLKRVHQTV

SKMLKQVEKYTNPHQLGRGKEHGLNFPKAPTKGSSR

SeqID 281

MDVSSSPNITFMLQYTEANPQYVDYTNREEAVKIDEELSLETNRQMIEGLTEDELTRIQEAVPETQLNFREYIDYMN

RSYATEEQSKELTAIFTQEADYLQKLRLIDLKNKLESAYQNGSLLWQGVISFDNAFLAEQGLYDVATGQVDQKAIKA

VMRDMMPTLIQKEGLSDSAFWWGNIHLNTDNIHIHFGLSEVESNREKIFYQPRGRMEYKGNFSQKTINRFKSGVYHG

LLKEETRSNLLRKEQILANLKADFITSIYQKDKITSSAEKNFLEQAYNHLPLNKKWRYGSNARDFAVSKFFLDRYLD

SYLNNEGSAAYQEFLKETRDFLQTYEGVYSAEKNKIYEKLRKVDGQTIRTLAESKGYDLEHHLARRVMDLRERLANN

ILRSFREAAPQIQDVQLEKNLESFSVLNQKKILEQHPEASVVKSQKAWQKLGYFVKAGEQPLEIIRPVYKSYDKHGK

GIGRPEFVSDTVYDISQLTENTQLKSLTLKDLSLFSSNELKELVDAAKLKTNPTERERRELGTYRYALKLSILESSQ

KELQVRQKLLEQVQPLASDQPFLDFKKQLIAQELQAIALQLTPNYKLSEDDKALKNRLKRQFEDSVALPVSKATPGA

IQLPIRQLWTELGLVHHIQDENILTLLKGTSTTKQAYIEELQTHISIFQLKYQINNRNKQISQLSDEATIKEMRIAN

AKGFSELKRLYDTLQPSDDGQNQISQAVSKQLQERKVIKKAQLQQTQRSGKINTDFMRQLTASLNRSQQASKKALMR

RARSDEREEQEERRQAQR

SeqID 282

MKKNKFLLVSIVFIIIFVVQPQNFQSLKNIFTQNDIASQLNISSSPEEKNDGLGTAYQTQNEDLKSKSFDGQHQVIV

VNEKAQFTAEELSMRNGSWEKYDNLDFLNRVGVAEAMLGKELMPKEARQDISSVKPTGWKNKKITFNGKQDYLYNRS

HLIGFQLSGENANVKNLFTGTRALNALNFNDDKSSMVYYENEVANYIKKTNHHVRYRTPLFKNVELVARGVRIEAQS

IEDETISFDVYIFNGQPGYDIDYLTGSSEKIMITK

SeqID 283

VTKEIKIRSIPEKTWAQLHMISEEYEYPSFNEFMLAQLQRIVENGGLDLYDNKFAETLAVIKEQQAQILDQLLKNEI

KLLAYHAKQDIVEELTTDWLRFMDDVDALAAERGAGGR

SeqID 284

LLYYFIYLIKVIGNGLKLSLICGINWLIKIVFKGQFYLFSAVFCGLLTYYMPQDIQLFTVRVLELIIMLKVIIDVTH

TALSRDFKRMKTPLFLGVMYVFFLAGNSYIKAHLLTEVMVNHLISFWLISLFFATLVIVIQPRLFKHYLLKKVIDKE

YLGIRKFTDSLPPEINLYKDADEEDADKRMRLINQNVIKHPYQEVVELSFLNREVITAIGYKAVPFEKETERTFIDD

DTIYYPIFTVHPFRNLEGKSDFYHILMKLKLSRKAAFTKNGERLLIRDF

SeqID 285

MIRNEFYNQLINSEPIGFIDPFTDLGEFDSIQMKFKQPVRNLVNKYSGKPYNLSWQNKIEQMRVLYIKYQKSLKLED

EEQEVHNRVKNKKSKKYVHEIVTTYLKLGFRFKEIEARVSLFNTRLRRNWKRSDYVTTDNPEFYLKKDLQNGYCSPN

SFLPRSMKIN

SeqID 286

MNEIKCPHCGTAFAINESEYHQLLEQIRGDAFDKEVSERLEKRRLILGEQAKNQLQEVVVEKDKEIAKLQYKVKQFL

IEKDNLLKDNEYQLAEQLNQKDMMLRDLENQIDRLRLEHENSLQEALTKVERERDAIQNQLHIQEKEKDLALASVKS

DYEVQLKAANEQVEFYKNFKAQQSTKAVGESLEHYAETEFNKVRHLAFPNAYFEKDNTLSSRGSKGDFIYREKDRND

LEFLSTMFEMKNESDDTIKKHKNEDFFKRLDKDRREKSCEYAVLVTMLEADNDYYNTGTVDVSHKYPKMYVIRPQFF

IQLIGILRNAALNTLKYKQELALMKEQNIDITHFEEDLDIFKNAFARNYNSASKNFQKAIDEIDKSIKRMEAVKAAL

TTSENQLRLANNKLDDVSVKKLTRKNPTMKAKFDALKD

SeqID 287

MNHFELFKLKKAGLTNLNINNIINYLKKNSLTSLSVRNMAVVSKCKNPTFFIENYKQLDLKKLRQEFKKFPVLSILD

SNYPLELKEIYNPPVLLFYQGNIELLSKPKLAVVGARQASQIGCQSVKKIIKETNNQFVIVSGLARGIDTAAHVSAL

KNGGSSIAVIGSGLDVYYPTENKKLQEYMSYNHLVLSEYFTGEQPLKFHFPERNRIIAGLCQGIVVAEAKMRSGSLI

TCERALEEGREVFAIPGNIIDGKSDGCHHLIQEGAKCIISGKDILSEYQ

SeqID 288

MTERTFEDIELDLKLFQIKLDNAENSKRLLQKLKNDVMELQIELLESLKLGDAYLTESEELEENNDFILTVNSETLS

LSYDNRINLVSKEIMDYENALDKLYYEKQSLMQKSNERKGG

SeqID 289

LFNKIGFRTWKSGKLWLYMGVLGSTIILGSSPVSAMDSVGNQSQGNVLERRQRDAENRSQGNVLERRQRDAENRSQG

NVLERRQRDAENRSQGNBLERRQRDAENRSQGNVLERRQRDVENKSQGNVLERRQRDAENKSQGNVLERRQRDAENR

SQGNVLERRQRDVENKSQGNVLERRQRDAENRSQGNVLERRQRDVENKSQGNVLERRQRDVENKSQGNVLERRQRDA

ENRSQGNVLERRQRDVENKSQGNVLERRQRDVENKSQGNVLERRQRDAENRSQGNVLERRQRDAENRSQGNVLERRQ

RDAENKSQVGQLIGKNPLLSKSIISRENNHSSQGDSNKQSFSKKVSQVTNVANRPMLTNNSRTISVINKLPKTGDDQ

NVIFKLVGFGLILLTSRCGLRRNEN

SeqID 290

MKRLTYYFKGYIKETIFGPLFKLLEASFELLVPIVIAKMIDETIPRGDRSSLLLQIGLIFFLAAVGVVVAITAQYYS

SKAAVGYTRQLTEDLYQKVMSLGKKDRDELGTASLITRLTADTFQIQTGLNQFLRLFLRAPIIVFGAIIMAFSISPS

LTIWFLVMVVTLFIIVFVMSRLLNPIYLKIRTSTDYLVKLTRQQLQGVRVIRAFNQVDRESEAFNDINYHYTNLQLK

AGRLSSLVTPLTFLVVNITLVVIIWRGNLNIANHLLSQGMLVALINYLLQILVELLKMTMLVTSLNQSYISAKRIIA

VFRRPSEIIDDKLERKYSNKALEVQEMAFSYPNSSEKALSDITFSMNVGETLGIIGGTGSGKSTLVNLLLHIYLVQE

GDIDIYHQGKSPDTISNWRTLVRVVPQNAQLFKGTIRSNLSLGLGKVSEEKLWTALEIAQASDFVKEKDGQLDAPVE

SFGRNFSGGQRQRLTIARALVQDKIPFLILDDATSALDYLTEARLFKAITKHFNQTNLIIVSQRINSIQNADRILLL

DKGKQVGFDNHQSLLAHNKVYKSIYHSQNFKEEE

SeqID 291

MKFNEQSNSQAALLGLQHLLAMYAGSILVPTMIASALGYNAEQLTYLIATDIFMCGIATLLQLQLSKHFGVGLPVVL

GCAFQSVAPLSIIGAQQGSGYMFGALIASGIYVVLVAGIFSKVANFFPPIVTGSVITTIGLTLIPVAMGNMGDNAKE

PSLQSLTLSLVTIGVVLLINIFAKGFLKSISILIGLISGTILAAFMGLVDASVVAEAPLVHIPKPFYFGAPRFEFTS

ILMMCIIATVSMVESTGVYLALSDITNDKLDSKRLRNGYRSEGLAVLLGGLFNTFPYTGFSQNVGLVQISGIRTRKP

IYFTALFLVILGLLPKFGAMAQMIPSPVLGGAMLVLFGMVALQGMKMLNQVDFEHNEHNFIIAAVSIAAGVGFNGTN

LFISLPNTLQMFLTNGIVISTLTAVVLNIILNGLPKKLI

SeqID 292

MANTYDLISQRIEAQRQKLIAIDIVAVASSLGLNLKQGSGGHLYWDEHDSFHIYPQTNTFRWWSRSMGTNTIDLVQV

IQEELTGKKPSFRETVNFLETGQFESVTVTPVVREPFKHYLAPYEHHNFDLGRQYLKEERGLSDETIDFALASGSMS

SATLKKGDYFEPVIIFKSFAEDGRMIGGSLQGIVENKVQHPERGRLKQIMKHSDGLAGFHLDVGTPKRLVFSEAPID

LLSYYELHKESLQNVRLVAMDGVKKGVISRYTADLLTDGQYSQTMPRESIRGAIDAINQTTRILKNNPNMITIAVDN

DEAGRNFIKELQEDGIPINVDLPPRKEHQSKMDWNNYLKQKKGLLKMPQTEGTQKAPEQVLEHEKMDRSQISSGSLE

DDPQGSAKPVSKRDTFEQAVTSHPTFSYPLLQFSTEEAFVSNVRDGYHIASEEDIRNLNYYAPSLQQTANWYRDNLA

DRQVTYMLKGDKEIKALQVSFAKDKFAHLTGIRPIGKGLSAEKLLDDFAKGRGSYPNLTLSNGFNDKIQVLPMIQEL

SQSKSFVFTDLEEVQKMRNLKASHAIQSNNRSLVVALKTIDDVTFPSSLLRGKKNLNDDLIQKAKENEVLGVLSEKD

GNITVLSVNDKYIQDGGQALKDMIKNGELEPLQMETIQRHVPHENAYPKDSDGDGLTDDEEIALGTNPFSSDSDGDG

TPDNVEKANGTDPTNASDNEVTRQQEANKRDFTLSEMIKAKNTAALNQHLQDGIKQYFDSDTYKQYLEGMAHFNNYS

PRNIQLIMSQFPEASMVASFQEWRKRNGSVKKGEKAIYIQAPVSVMKKDENGKPILNPETGEKETITTFKPVPVFDI

KQVSPQEGKELNLPKAMGTIPEQLDKEYYQNVYRSLRDISQNNNKVPIRFRELGQEDGFYSPQTNEIVIKKGMSYER

TLSTLIHEMAHSELHNKQSLTERFDGKLTRSTKELQAESIAYVVSSHLGFDTSQESFPYLASWSKEKDGLANLTAQL

EIVQEEAKNLMERIDQQLSQYQTVTLNKETQQLTKQEMKKQTHPFYQSLAAAKTSRAQVTTQEKEASVKKDNRPTMP

SeqID 293

MNSQETKGHGFFRKSKAYGLVCGIALAGAFTLATSQVSADQVTTQATTQTVTQNQAETVTSTQLDKAVDTAKKAAVA

VTTTTAVNHATTTDAQADLANQTQAVKDVTAKAQANTQAIKDATAENAKIDAENKAEAERVAKANKAGQAEVDARMK

AGQAAVDARNKAKQQAQDDQKAKIDAENKAESQRVSQLNAQNKAKIDAENKDAQAKADATNAQLQKDYQTKLANIKS

VEAYNAGVRQRNKDAQAKADATNAQLQKDYQAKLALYNQALKAKAEADKQSINNVAFDIKAQAKGVDNAEYGNSIMT

AKTKPDGSFEFNHDMIDGVKTIGYGKLTGKVNHHYVANKDGSVTAFVDSVTLYKYEYRNVAQNAAVNQNIVFRVLTK

DGRPIFEKAHNGNKTFAETLNKTLQLNLKYELKPHASSGNVEVFKIHDDWVHDTHGSALVSYVNNNDAVPNVVIPEQ

PTPPKPEKVTPEAEKPVPEKPVEPKLVTPVLKTYTPVKFIPREYKPVPSTPETFTPEKFTPAQPKVKPHVSVPEKIN

YKVAVHPVQIPKATPTKKVLDENGQSINGKSVLPNATLDYVAKQNESQYKGIKASAEAIAKGFAFVDQPNEALAELT

VKSIKASNGDDVSSLLEMRHVLSKDTLDQKLQSLIKEAGISPVGEFYMWTAKDPQAFYKAYVQKGLDITYNLSFKVK

KEFTKGQIKNGVAQIDFGNGYTGNIVVNDLTTPEVHKDVLDKEDGKSINNGTVKLGDEVTYKLEGWVVPANRGYDLF

EYKFVDHLQHTHDLYLKDKVVAKVAITLKDGTVIPKGTNLVQYTETVYNKETGRYELAFKADFLAQVSRSSAFGADD

FIVVKRIKAGDVYNTADFFVNGNKVKTETVVTHTPEKPKPVMPQKVTPKAPALPSTGEQGVSVLTVLGAALLSLLGL

VGFKKRQQ

SeqID 294

MNQIKIITGLTVATLSAVVGNVYAEDITPTAPVNEPQVSSETAKTPQVTESQVNSAKVTADQATSDVNAQKNVVNNA

QNQKNQAQQKLVNATTTLNETQKLVQESTNQNQVQQTVDSAKQRLSQTEANQKITQTEQVKAQNQVNAQQTVVVNNE

HDVATKTADVKQAQASVDTAKDALTNTIVNSDLNKAQSNVTTKTADVKTATDALTKAQATDKTLTNQKAKAQQIVDS

AKQNLSAKDTQLSQANAEVNHHKFKTALGQSHYYNQRDNAWAGVYGGHTFASTGCVPSALAMVYSDLSNRTITPREI

ADYLYNNTDEFNKRFGGTSGKGIISATKAFGYVVTHLASKNAITEALKAGHHVVAAVQNNKFSPWGPQYSHRIVLRG

SSNGNTYVYDPYNRDNNGFYSVDRIWNEQSRDSIDTAGVGVPFFAIMTKNMANALTKQSQALASQQVAQKQTNDAQA

KATGLNAVTMQTPIAQANLIKAQSNLKDAQKRALEAQASVKLANQDNVKKQADLTKAESKLKDAQKQLAAAQAKLTT

SKTKLNQLKQVLAEASQQVAQANQDYKQAKDNLTQKTAYLTNLRNAQANLIKAQSDVAQAKDNLANKIAKLQREVAY

LQELKTKAVDAQSQYQKVLSAYKSVLSAKASLKLAEEKARLDKKGHEAVAVVDETGKITSYITSKHKIEMKSLVATK

TTDVQVSVAKASVLPSTGDVKQVSVALLGMLLTFSGFLGIRKQSKKVIN

SeqID 295

MISRKVALVTGASAGFGAAIVTKLVSDGYSVIGCARRMDKLKCFGEKFSEGYFYPLQMDITSRESVDKALESLPKNL

QSIDLLVNNAGLALGLDKSYEADFEDWMTMINTNVVGLIYTRRCILPKMVEVNRGLIINLGSTAGTIPYPGANVYGA

SKAFVKQFSLNLRADLAGTKIRVTNLEPGLCEGTEFSTVRFKGDHKRVEKLYEGAHAIQAEDIANTVSWVASQPEHI

NINRIEIMRVSQTYGPQPVRD

SeqID 296

MIYLDNAATTALTPSVIEKMTNVMTSNYGNPSSIHTFGRQANQLLRECRQIIAEYLNVNSREIIFTSGGTESNNTAI

KGYALANQLKGKHIITSEIEHHSVLHTMTYLSERFGFDITYLKPNHGQITAKDVQEALRDDTTMVSLMFANNETGDF

LPIQEIGQLLRNHQAVFHVDAVQVFSKMELDPHSLGIDFLAASAHKFHGPKGVGILYCAPHHFDSLLHGGDQEEKRR

ASTENIIGIAGMSQALTDATTNTLKNWTHISQLRTTFLDAISDLDFYLNNGQDCLPHVLNIGFPRQNNGLLLTQLDL

AGFAVSTGSACTAGTVEPSHVLTSLYGANSPRLNESIRISFSELNTQEEILELAKTLRKIIGD

SeqID 297

MSRKTFKHILSIGVCTLVLSMSLYYTEKAHAIAGPSDRQYVENPNPHIIVNVTGTDQNGNSILPHYIEVNVRMGQTL

SKEEILDYIARNLNSSVGGESKNVQYSNIEFKESAYLKRQLDDGKTEEIAIDNDGVTVPKDGPNKFWIDVPVTCTVT

PIVTETHFVRWGTPVAISHRIYFVEESSGKVLDEYTNLHTADSELNGYRVGDYITDYALSKSAYEAFLNSRLDKEGY

KLQHRISTNVRQNLQIDKLIFNYDFNEENIYYQIGNIRPLLSRSSAEVESDIITERYYVSKNAKSLARTESTISIKM

VDAKTEQPLFNHTLTGYQLATVSHVYNRLFEENLIPTTKSGERYFIQNMKKTAEQEYTVYLSETPYSKENAPVISYD

ARPVDWDYHSGASGSLENQRNIYTEEDSTEFLGNKPQAACYPNKQFACENTDSKYNYSYLEK

SeqID 298

MNPLIIGMNDKQAEAVQTTDGPLLIMAGAGSGKTRVLTHRIAYLIDEKYVNPWNILAITFTNKAAREMRERAIALNP

ATQDTLIATFHSMCVRILRREADYIGYNRNFTIVDPGEQRTLMKRIIKQLNLDTKKWNERSILGTISNAKNDLLDEI

AYEKQAGDMYTQVIAKCYKAYQEELRRSEAMDFDDLIMMTLRLFDQNKDVLAYYQQRYQYIHVDEYQDTNHAQYQLV

KLLASRFKNICVVGDADQSIYGWRGADMQNILDFEKDYPQAKVVLLEENYRSTKKILQAANNVINHNKNRRPKKLWT

QNDEGEQIVYHRANNEQEEAVFVASTIDNIVREQGKNFKDFAVLYRTNAQSRTIEEALLKSNIPYTMVGGTKFYSRK

EIRDVIAYLNILANTSDNISFERIVNEPKRGVGPGTLEKIRSFAYEQNMSLLDASSNVMMSPLKGKAAQAVWDLANL

ILTLRSKLDSLTVTEITENLLDKTGYLEALQVQNTLESQARIENIEEFLSVTKNFDDNPEITVEGETGLDRLSRFLN

DLALIADTDDSATETAEVTLMTLEAAKGLEFPVVFLIGMEEGVFPLSRAIEDADELEEERRLAYVGITRAEQILFLT

NANTRTLFGKTSYNRPTRFIREIDDELIQHQGLARPVNSSFGVKYSKEQPTQFGQGMSLQQALQARKSNSQPQVTAQ

LQALNANNSHETSWEIGDVATHKKWGDGTVLEVSGSGKTQELKINFPGIGLKKLLASVAPISKLEN

SeqID 299

MKLYVQLMVILTFSFAGEVLSTIFNLPVPGSIIGLILLFLALKYKIIRLRHIDAVGNFLLANMTILFLPPAVGLMEH

FQDIKPYLFGIAIIILGALFLNILTIGLVSQWIKKRYEGDYPEIGGKNGNFNE

SeqID 300

MIFVTVGTHEQQFNRLIKEVDRLKGTGAIDQEVFIQTGYSDFEPQNCQWSKFLSYDDMNSYMKEAEIVITHGGPATF

MSVISLGKLPVVVPRRKQFGEHINDHQIQFFKKIAHLYPLAWIEDVDGLAEVLKRNIATEKYQGNNDMFCHKLEKII

GEI

SeqID 301

MVMKIIELKEATVQVSNGLAEMKTILDHVNLSIYEHDFITILGGNGAGKSTLFNVIAGTLMLSGGNIYIMGQVTTNL

PAEKRAKYLSRVFQDPKMGTAPRMTVAENLLVAKFRGEKRPLVPRKITNYTEEFQKLIARTGNGLDRHLETPTGLLS

GGQRQALSLMATLKKPNLLLLDEHTAALDPRTSVSLMGLTDEFIKQDSLTALMITHHMEDQALKYGNRILVMKDGKI

VRDLNQAQKNKMAIADYYQLFD

SeqID 302

MEIKKKHRIMLKSALILGTILVNNSYQAKAEELTKTTSTSQIRDTQTNNIEVLQTESTTVKETSTTTTQQDLSNPTA

STATATATHSTMKQVVDNQTQNKELVKNGDFNQTNPVSGSWSHTSAREWSAWIDKENTADKSPIIQRTEQGQVSLSS

DKGFRGAVTQKVNIDPTKKYEVKFDIETSNKAGQAFLRIMEKKDNNTRLWLSEMTSGTTNKHTLTKIYNPKLNVSEV

TLELYYEKGTGSATFDNISMKAKGPKDSEHPQPVTTQIEESVNTALNKNYVFNKADYQYTLTNPSLGKIVGGILYPN

ATGSTTVKISDKSGKIIKEVPLSVTASTEDKFTKLLDKWNDVTIGNHVYDTNDSNMQKINQKLDETNAKNIKTIKLD

SNHTFLWKDLDNLNNSAQLTATYRRLEDLAKQITNPHSTIYKNEKAIRTVKESLAWLHQNFYNVNKDIEGSANWWDF

EIGVPRSITATLALMNNYFTDAEIKTYTDPIEHFVPDAGYFRKTLDNPFKALGGNLVDMGRVKIIEGLLRKDNTIIE

KTSHSLKNLFTTATKAEGFYADGSYIDHTNVAYTGAYGNVLIDGLTQLLPIIQETDYKISNQELDMVYKWINQSFLP

LIVKGELMDSMRGRSISREAASSHAAAVEVLRGFLRLANMSNEERNLDLKSTIKTIITSNKFYNVFNNLKSYSDIAN

MNKMLNDSTVATKPLKSNLSTFNSMDRLAYYNAEKDFGFALSLHSKRTLNYEGMNDENTRDWYTGDGMFYLYNSDQS

HYSNHFWPTVNPYKMAGTTEKDAKREDTTKEFMSKHSKDAKEKTGQVTGTSDFVGSVKLNDHFALAAMDFTNWDRTL

TAQKGWVILNDKIVFLGSNIKNTNGIGNVSTTIDQRKDDSKTPYTTYVNGKTIDLKQASSQQFTDTKSVFLESKEPG

RNIGYIFFKNSTIDIERKEQTGTWNSINRTSKNTSIVSNPFITISQKHDNKGDSYGYMMVPNIDRTSFDKLANSKEV

ELLENSSKQQVIYDKNSQTWAVIKHDNQESLINNQFKMNKAGLYLVQKVGNDYQNVYYQPQTMTKTDQLAI

SeqID 303

MHSFSNPGYPYDNAVTEAFFKYLKHRQINRKHYQNIKQVQLDCFEYIENFYNNYNPHTANLGLTPNQKEENYFNAIK

SeqID 304

VKKTYGYIGSVAAILLATHIGSYQLGKHHMGLATKDNQIAYIDDSKGKAKAPKTNKTMDQISAEEGISAEQIVVKIT

DQGYVTSHGDHYHFYNGKVPYDAIISEELLMTDPNYRFKQSDVINEILDGYVIKVNGNYYVYLKPGSKRKNIRTKQQ

IAEQVAKGTKEAKEKGLAQVAHLSKEEVAAVNEAKRQGRYTTDDGYIFSPTDIIDDLGDAYLVPHGNHYHYIPKKDL

SPSELAAAQAYWSQKQGRGARPSDYRPTPAPGRRKAPIPDVTPNPGQGHQPDNGGYHPAPPRPNDASQNKHQRDEFK

GKTFKELLDQLHRLDLKYRHVEEDGLIFEPTQVIKSNAFGYVVPHGDHYHIIPRSQLSPLEMELADRYLAGQTEDND

SGSEHSKPSDKEVTHTFLGHRIKAYGKGLDGKPYDTSDAYVFSKESIHSVDKSGVTAKHGDHFHYIGFGELEQYELD

EVANWVKAKGQADELAAALDQEQGKEKPLFDTKKVSRKVTKDGKVGYMMPKDGKDYFYARDQLDLTQIAFAEQELML

KDKKHYRYDIVDTGIEPRLAVDVSSLPMHAGNATYDTGSSFVIPHIDHIHVVPYSWLTRDQIATVKYVMQHPEVRPD

VWSKPGHEESGSVIPNVTPLDKRAGMPNWQIIHSAEEVQKALAEGRFATPDGYIFDPRDVLAKETFVWKDGSFSIPR

ADGSSLRTINKSDLSQAEWQQAQELLAKKNTGDATDTDKPKEKQQADKSNENQQPSEASKEEKESDDFIDSLPDYGL

DRATLEDHINQLAQKANIDPKYLIFQPEGVQFYNKNGELVTYDIKTLQQINP

SeqID 305

MKKGFFLMAMVVSLVMIAGCDKSANPKQPTQGMSVVTSFYPMYAMTKEVSGDLNDVRMIQSGAGIHSFEPSVNDVAA

IYDADLFVYHSHTLEAWARDLDPNLKKSKVDVFEASKPLTLDRVKGLEDMEVTQGIDPATLYDPHTWTDPVLAGEEA

VNIAKELGRLDPKHKDSYTKKAKAFKKEAEQLTEEYTQKFKKVRSKTFVTQHTAFSYLAKRFGLKQLGISGISPEQE

PSPRQLKEIQDFVKEYNVKTIFAEDNVNPKIAHAIAKSTGAKVKTLSPLEAAPSGNKTYLENLRANLEVLYQQLK

SeqID 306

LRKKQKLPFDKLAIALMSTSILLNAQSDIKANTVTEDTPATEQTVETPQPTAVSEEAPSSKETKTPQTPSDAGETVA

DDANDLAPQAPAKTADTPATSKATIRDLNDPSQVKTLQEKAGKGAGTVVAVIDAGFDKNHEAWRLTDKAKARYQSKE

DLEKAKKEHGITYGEWVNDKVAYYHDYSKDGKTAVDQEHGTHVSGILSGNAPSETKEPYRLEGAMPEAQLLLMRVEI

VNGLADYARNYAQAIRDAINLGAKVINMSFGNAALAYANLPDETKKAFDYAKSKGVSIVTSAGNDSSFGGKTRLPLA

DHPDYGVVGTPAAADSTLTVASYSPDKQLTETATVKTADQQDKEMPVLSTNRFEPNKAYDYAYANRGTKEDDFKDVK

GKIALIERGDIDFKDKIAKAKKAAGVGVLIYDNQDKGFPIELPNVDQMPAAFISRKDGLLLKDNPQKTITFNATRKV

LPTASGTKLSRFSSWGLTADGNIKPDIAAPGQDILSSVANNKYAKLSGTSMSAPLVAGIMGLLQKQYETQYPDMTPS

ERLDLAKKVLMSSATALYDEDEKAYFSPRQQGAGAVDAKKASAATMYVTDKDNTSSKVHLNNVSDKFEVTVTVHNKS

DKPQELYYQATVQTDKVDGKHFALAPKALYETSWQKITIPANSSKQVTVPIDASRFSKDLLAQMKNGYFLEGFVRFK

QDPKKEELMSIPYIGFRGDFGNLSALEKPIYDSKDGSSYYHEANSDAKDQLDGDGKQFYALKNNFTALTTESNPWTI

IKAVKEGVENIEDIESSEITETIFAGTFAKQDDDSHYYIHRHANGKPYAAISPNGDGNRDYVQFQGTFLRNAKNLVA

EVLDKEGNVVWTSEVTEQVVKNYNNDLASTLGSTRFEKTRWDGKDKDGKVVANGTYTYRVRYTPISSGAKEQHTDFD

VIVDNTTPEVATSATFSTEDRRLTLASKPKTSQPVYRERIAYTYMDEDLPTTEYISPNEDGTFTLPEEAETMEGATV

PLEMSDFTYVVEDMAGNITYTPVTKLLEGHSNKPEQDGSDQAPDKKPEAKPEQDGSGQTPDKKTETKPEKDSSGQTP

GKTPQKGQPSRTLEKRSSKRALATKASTRDQLPTTNDKDTNRLHLLKLVMTTFFLGLVAHIFKTKRQKETKK

SeqID 307

FSVTYSQSERTVVFSFGEITFSRSRWTNGFETRIPVDEWLGLEKYKRYSIEFLYHVAKLATMMPYRQVCKVIDSTLQ

TIITKDCVLKAVKFVEKLLKEKERYRFYLEEPPERKKVKKLYVEGDGVMIKSTDSREERRYLDLTHFVIHTGSKKVS

TKRYELQDKHEILQLNYDKAKYNLLDYIYNNYEVDDDTILITNSDMGKGYTSRVFKELGKALKVKKHEHFWDIYHVK

EKLSSYLRKYPIELTDFALDAVKKYNSDKLELVFDTVESLICDELEDQEFQKFKKKVLNNFKYIKPAHLRNLSNRGI

GIMESQHRKITYRMKRRGMYWSKWGISTMANMIILERANGLRELFFGSWRKVYSEYKEGSFSAGRLFKKTDELDKFS

KPLLKNGRKWSITGIKTK

SeqID 308

KASSGDYGTKREIITANKDKYSISKMCRWLNMPRSSYYYQAVESVSKTEFEETIKRIFLDSESRYGSRKIKICLNNE

GITLSRRRIRRIMKRLNLVSVYQKATFKPHSRGKNEAPIPNHLDRQFKQERPLQALVTDLTYVRVGNRWAYVCLIID

LYNREIIGLSLGWHKTAELVKQAIQSIPYALTKVKMFHSDRGKEFDNQLIDEILEAFGITRSLSQAGCPYDNAVAES

TYRAFKIEFVYQETFQSLEELALKTEKATLFCTTFIKCCLFRFLPML

SeqID 309

MKTRNRKGGYLANTANEYIDSKQAIHCLSVELEPQIRFEEGQPTGEIIAYKAWFSQKGLPPFMVKFENEVTLPAYMV

MVQFENLQACEVGFNVYFKADNLKEVK

SeqID 310

LSTFDSVTGRTNHLEAYEGLNLSEKFAILSHFDKLRNELQTPSIQLGEFDREMEAFSITLGNELLGYLEANGSPYEL

KRELNQAEMMAVLELSRQLVAKFSTKLEELGIDLGSFQPDQVNILLDAVGRFRLKNADIALLGGYPKASVSQLALAT

ELLQMGLSHDKVEFFLTSQLQLEDMRQVAFAFLHESLTREEAEQFETDRFRHTSLNFREWRELLEKQEPEMVEMSDV

SPLVREVLHHYPLGSRVTYKGQEFEILSIEAADMDNLIRIELQNDFSYLIEQNPVLYEQNLAEIRQVLHLSRSEIVE

REEQPEEEWTLFSFMDEGTEDNEKEPEIVFESTDKVVTLDSQPAQVSETISESVPETKEVIEAEQAIAVDFSFPEDL

TNFYPKTARDKVVANLVAIRLVKELESANQSATPNEQEILAKYVGWGGLANEFFDEYNPKFSYEREELKTLVSDKEY

SDMKQSSLTAYYTDPLLIRQMWSKLEQDGFSGGKILDPSMGTGNFFAAMPAHLREKSELCGVELDTITGAIAKQLHS

NVHIEVKGFETVAFNDNSFDLVISNVPFANIRIADNQYDKPYMIHDYFVKKSLDLVHDGGQVAIISSTGTMDKRTEN

ILQDIRETTDFLGGVRLPDTAFKAIAGTSVTTDMLFFQKHLNKGYQADDIAFSGSVRYDKDERIWLNPYFDGEYNAQ

VLGRYEIKNFNGGTLSVKETTDNLIASVREALQHVKAPRVIDKTEVMINSDVIARQVIDTTIPPEIRENLEQYSYGY

KGSTIYYRDNKGIRVGTKTEEISYYVDDEGNFQAWESKHSQKQIDRFNNLEVTDSTALDVYVTEEPAKRGQFKGYFK

KAVFYEAPLSEKEVARIKGMVDIRNAYQEVIAIQRNYDYDKNEFNRLLGNLNRTYDSFVKRYGFLNSPVNRNLFDSD

DKYSLLASLEDESLDPSGKTVIYTKSLAFEKALVRPEKMVKEVSTALDALNSSLADGRGVDFDYMASIYQTASKAAL

IEELGDQIIPDPESYLKGQLTYVSRQEFLSGDIVTKLEVMDLLLKQDNHDFNWAHYGNLLESVRPARVMLADIDYRI

GSRWIPLAVYGKFVQEAFMGKNYDLTATEVEEVLSVSPIDGTMEFRTRFAYTYSTATDRSLGVAGSRYDSGRKIFEN

LLNSNQPTITKQIQEGDKKKNVTDVEKTTVLRAKEAQIQDLFQDFVASYPEAQQMIEDTYNSLYNRTVSKVYDGSRL

EIDGLAQNISLRPHQKNAIQRIVEEKRALLAHEVGSGKTLTILGAGFKLKELGMVHKPLYVVPSSLTAQFGQEIMKF

FPTKKVYVTTKKDFAKARRKQFVSRIITGDYDAIVIGDSQFEKIPMSQEKQVTYIQDKLEQLREIKQGSDSDYTVKE

AERSIKGLENQLEELQKLDRDTFIEFENLGIDFLFVDEAHHFKNIRPITGLGNVAGITNTTSKKNVDMEMKVRQIQA

EYGDRNVVFATGTPVSNSISELYTMMNYIQPDVLERYQVSNFDSWVGAFGNIENSMELAPTGDKYQPKKRFKKFVNL

PELMRIYKETADIQTSDMLDLPVPEATVIAVESELTEAQKNYLEELVDRSDAIKSGSVDPSVDNMLKVIGEARKLAI

DMRLIDPAYTLSDNQKIMQVVDNVERIYREGKGDKATQMIFSDIGTPKSKEEGFDVYNELKALLVDRGIPKEEIAFV

HDANTDEKKNSLSRKVNSGEVRILMASTEKGGTGLNVQARMKAVHHLDVPWRPSDIQQRNGRLIRQGNQHQNVEIYH

YITKGSFDNYLWATQENKLRYIKQIMTSKDPVRSAEDIDEQTMTASDFKALATGNPYLKLKMELENELTVLENQKRA

FNRSKDEYRHTISYCEKNLPVMEKRLRQYDRDIEKSQATKNQEFIMRFDNQTIDNRSEAGDYLRKLITYNRSETKEV

RTLATFRGFELKMATRSPGEPLSDMVSLTISGDNQYSVSLDLKSDVGTIQRINNAIDHILEDKEKTEEMTNNLKDKL

AVARVEVEKVFAKEEEYQLVKAKYDVLAPLVEREADLEEIDVALSQFSSSDPCLKKDDQQLVLDI

SeqID 311

MTTKCNHHFLINQEKGEKYVFRKSKQYRTLCSVALGTVVMAFVALAGPMVQADEVGRTVATSVQTETNPATNLKENQ

PSPIAEQKDSLAATGQSTGTVTVTVPHDKVTQAVDKAKTEGIKAVQDKPMDLGNTVSAAETSQQLKKAEEDATNQTT

TISKTVEIYKSDKATYEAEKKWVEKRNEELTAAYDKAEQTGTGLNHSVDTTVSELKSQDQNAHVTVNTQTVKSGDGT

SVSGYQEYVKSVAAIDKKNKANLADYRTKKQAADAVVAKNQLIQKENEAGLAKAKAENEAIDRRNKEGQKAVDEANK

AGQAAVEQANQEKQKQAANRAFEIATITKRNKEREEVAKKENAAIDAYNAKEWIRYKRDLANISKGEEGYISEALAQ

ALDLNHGEPQVKHGAGTRNPDRIISKGDAMLGGYSNILDSTGFFVYNHFKTGETLNFTYQNLKHARFDGKKITAITY

DITNLVSPTGTNAVQLVVPNDPTEGFIAYRNDGAGNWRTDKMEFRVKARYFLEDGSQVTFTKEKPGVFTHSSLNHND

IGLEYVKDSSGKFVPIHGSSVQVTNEGLARSLGSNRASDLKLPEEWDTTSSRYAYKGAIVSTVTSGNIYTVTFGQGD

MPTQVGGKTYWFALNTLPVAKTVTPYNPKTHVRPQLDPVPEPIKVTPETYTPKIFTPEKPVTFTPKSVEKVPQPSLT

LTKVTLPTNLKLEPLPKAPQKPTVHYHDYLLTTTPAIAKEVMNVDKVNLHGKQVAKDSTVIYPLTVDVLSPNRSKIT

SLIFEDYLPAGYAFDMTKTQAENSDYDLTFDKNKNFVTLKAKDSLLQTLNKELNKSYQLSAPKLYGSVQNDGATYSN

SYKLLINKDTPNTYTVISNVVRIRTPGDGETTSRIRPKKDNENADGVLINDTVVALSTTNHYRLTWDLDQYKGDTSS

KDTIARGFLFVDDYPEEALDLVDKGTVITILDGKAVSGISVYSYTSLDKAPKELQDKLARANISPKGAFQVFEPDNH

QAFYDTYVKTGQSLALLTKMKVKDSLYGQTYRYKNKAYQVDFGNGYETKEVVNTVVHPEPKKQNLNKDKVDINGKAM

LVGSQNFYTLSWDLDQYRGLQADKSQIAQGFYFVDDYPEDVLLPDTKAIQIMTKDGKAVKGMEIKTYHQLSDAPKEL

QAALAKRNITPKGAFQVFMPKDPQAFYKAYVTTGQNLTIVNPMTVREAVYNSGKSYDNVAYQVDFGQAYETNIVTNH

VPTVNPHKSNTNKEGVSIEGKTVLPNTVNYYKIVLDYSQYKNMIVTDDVLVKGFYMVDDYPEEALTPHPDGTQVMDQ

NGKFVRGLSVRTYASLADAPKAVQEAMKSRNFIPKGAIQVFQADDPKTFFETYVKTGQKLVVTTRMTVKNERIQTGG

QYENTAYQIDFGIAYVTETVVNNVPKLEPQKDVVIDLSQKDKSLNKGAIALDQVFNYRLVGSLIPRNRATALVEYSF

KDDYDEKHDEYKGVYKAYTLRDVTLKDGTVLKQGTEVTKYTLQSVDKAKGTIAIRFDTAFLENIADESEFQAELYLQ

MKRIASGDVENTVIHSVNGYNIRSNTVKTTTPQPESPTPDNPPSPQPPVPTTESPVQASVLPSTGESQSLLALIGGG

LLLGLAYGLSKRKKEKN

SeqID 312

MANNNFYNRDPFGNMDDIFNSLMGNMGGYNSENKRYLINGREVTPEEFSQYRQTGKLPGQELNNQNTPTNQVSADSV

LTKLGTNLTDQARQHLLDPVIGRNKEIQETAEILARRTKNNPVLVGDAGVGKTAVIEGLAQAIINGDVPAAIKNKEI

ISIDISSLEAGTQYRGSFEENIQNIIKEVKETGNIILFFDEIHQILGAGSTGGDSGSKGLADILKPALSRGELTVIG

ATTQDEYRNTILKNAALARRFNEVKVNAPSAQDTFNILMGIRNLYEQHHNVVLPDSVLKAAVDLSIQYIPQRSLPDK

AIDLIDMTAAHLAAQHPVTDLKSLEKEIAAQRDKQEKAVNTEDFEEALKVKTRIEELQNQIDNHTEGQKVTATINDI

AMSIERLTGVPVSNMGASDIERLKELGNRLKGKVIGQNDAVEAVARAIRRNRAGFDDGNRPIGSFLFVGPTGVGKTE

LAKQLAFDMFGSKDAIVRLDMSEYNNRTAVSKLIGATAGYVGYDDNSNTLTERIRRNPYSIVLLDEIEKADPQVITL

LLQVLDDGRLTDGQGNTINFKNTVIIATSNAGFGNEAFTGDSDKDLKIMRRISPYFRPEFLNRFNGVIEFSHLSKDD

LNEIVDLMLDEVNQTIGKKGIDLVVDENVKSHLIDLGYDEAMGVRPLRRVIEQEIRDRITDYYLDHTDVKHLKANLQ

DGQIVISER

SeqID 313

MGRFKELLESKKALILHGALGTELESRGCDVSGKLWSDKYLIEDPAAIQTIHEDYIRAGADIVTTSTYQATLQGLAQ

VGVSESQAEDLIRLTVQLAKAVREQVWKSLTKEEKSERIYPLISGDVGPYAAFLADGSEYTGLYDIYKEGLKNFHRH

RIELLLDEGVDLLALETIPNAQEAEALIELLVEDFPQVEAYMSFTSQDGKTISDGSAVAGLAKAIDVSPQVVALGIN

CSSPSLVADFLQAIAEQTDKPLVTYPNSGEIYDGASQSWQSSRDHSHTLLENTSDWQKLGAQVVGGCCRTRPADIAD

LSEHLT

SeqID 314

MKIGIDKIGFATSQYVLEMTDLAIARQVDPEKFSKGLLLDSLSITPVTEDIVTLAASAANDILSDEDKETIDMVIVA

TESSIDQSKAASVYVHQLLEIQPFARSFEMKEACYSATAALDYAKLHVEKHPDSKVLVIASDIAKYGIKSTGESTQG

AGSIAMLISQNPSILELKEDRLAQTRDTMDFWRPNYSDVPYVNGMFSTKQYLDMLKTTWKEYQKRFNTSLSDYAAFC

FHIPFPKLALKGFNKILDNNLDEQKKAELQENFEHSITYSKKIGNCYTGSLYLGLLSLLENSQNLKAGDQIAFFSYG

SGAVAEIFTGQLVDGYQNKLQSDRMDQLNKRQKITVTEYEKLFFEKTILDENGNANFNTYRTGTFSLDSICEHQRIY

KKINN

SeqID 315

MKSAYIFFNPKSGKDEQALAQEVKSYLIEHDFQDDYVRIITPSSVEEAVALAKKASEDHDDLVIPLGGDGTINKICG

GVYAGGAYPTIGLVPAGTVNNFSKALNIPQERNLALENLLNGHVKSVDICKVNDDYMISSLTLGLLADIAANVTSEM

KRKLGPFAFVGDAYRILKRNRSYSITLAYDNNVRSLRTRLLLITMTNSIAGMPAFSPEATIDDGLFRVYTMEHIHFF

KLLLHLRQFRKGDFSQAKEIKHFHTNNLTISTFKRKKSAIPKVRIDGDPGDQLPVKVEVIPKALKFIIPNSLP

SeqID 316

MTTFTAKFIDQEWEVPVESGRYHMIVGEFCPYAQRPQIARQLLGLDKHISISFVDDVPSDIGLIFSQPEQVTGAKSL

RDIYHLTDPTYKGPYTIPILIDKTKNRIVCKESADMLRLFTTDFSDLHQEDAPVLFSQETASLIDNDIKDINNMFQS

LMYKLAFLDKQADYDTYSKEFFTFLDQKEHLLGQRPFLLGDNLSEVDIHFFTPLVRWDIAGRDLLLLNQKALEDYPN

IFSWAKTLYNDFNLKTLTNPQSIKNNYYLGKFGRAVRHHTIVPTGPNMVKWEK

SeqID 317

MKKKIILKSSVLGLVAGTSIMFSSAFADQVGVQVIGVNDFHGALDNTGTANMPDGKVTNAGTAAQLDAYIDDAQKDF

KQTNPNGESIRVQAGDMVGASPANSGLLQDEPTVKTFNAMNVEYGTLGNHEFDEGLAEYNRIVTGKAPAPDSNINNI

TKSYPHEAAKQEIVVANVIDKVNKQIPYNWKPYAIKNIPVNNKSVNVGFIGIVTKDIPNLVLRKNYEQYEFLDEAET

IVKYAKELQAKNVKAIVVLAHVPATSKDDIAEGEAAEMMKKVNQLFPENSVDIVFAGHNHQYTNGLVGKTRIVQALS

QGKAYADVRGVLDTDTQDFIETPSAKVVAVAPGKKTGSADIQAIVDQANTIVKQVTEAKIGTAEVSGMITRSVDQDN

VSPVGSLITEAQLAIARKSWPDIDFAMTNNGGIRADLLIKPDGTITWGAAQAVQPFGNILQVVEITGRDLYKALNEQ

YDQKQNFFLQIAGLRYTYTDNKEGGEETPFKVVKAYKSNGEEINPDAKYKLVINDFLFGGGDGRASFRNAKLLGAIN

PDTEVFMAYITDLEKAGKKVSVPNNKPKIYNTMKMVNETITQNDGTHSIIKKLYLDRQGNIVAQEIVSDTLNQTKSK

STKINPVTTIHKKQLHQFTAINPMRNYGKPSNSTTVKSKQLPKTNSEYGQSFLMSVEGVGLIGIALNTKKKHMK

SeqID 318

MAYIWSYLKRYPNWLWLDLLGAMLFVTVILGMPTALAGMIDNGVTKGDRTGVYLWTFIMFIFVVLGIIGRITMAYAS

SRLTTTMIRDMRNDMYAKLQEYSHHEYEQIGVSSLVTRMTSDTFVLMQFAEMSLRLGLVTPMVMIFSVVMILITSPS

LAWLVAVAMPLLVGVVLYVAIKTKPLSERQQTMLDKINQYVRENLTGLRVVRAFARENFQSQKFQVANQRYTDTSTG

LFKLTGLTRPLFVQIIIAMIVAIVWFALDPLQRGAIKIGDLVAFIEYSFHALFSFLLFANLFTMYPRMVVSSHRIRE

VMDMPISINPNAEGVTDTKLKGHLEFDNVTFAYPGETESPVLHDISFKAKPGETIAFIGSTGSGKSSLVNLIPRFYD

VTLGKILVDGVDVRDYNLKSLRQKIGFIPQKALLFTGTIGENLKYGKADATIDDLRQAVDISQAKEFIESHQEAFET

HLAEGGSNLSGGQKQRLSIARAVVKDPDLYIFDDSFSALDYKTDATLRARLKEVTGDSTVLIVAQRVGTIMDADQII

VLDEGEIVGRGTHAQLIENNAIYREIAESQLKNQNLSEGE

SeqID 319

MKKGQVNDTKQSYSLRKYKFGLASVILGPFIMVTSPVFADQTTSVQVNNQTGTSVDANNSSNETSASSVITSNNDSV

QASDKVVNSQNTATKDITTPLVETKPMVEKTLPEQGNYVYSKETEVKNTPSKSAPVAFYAKKGDKVFYDQVFNKDNV

KWISYKSFCGVRRYAAIESLDPSGGSETKAPTPVTNSGSNNQEKIATQGNYTFSHKVEVKNEAKVASPTQFTLDKGD

RIFYDQILTIEGNQWLSYKSFNGVRRFVLLGKASSVEKTEDKEKVSPQPQARITKTGRLTISNETTTGFDILITNIK

DDNGIAAVKVPVWTEQGGQDDIKWYTAVTTGDGNYKVAVSFADHKNEKGLYNIHLYYQEASGTLVGVTGTKVTVAGT

NSSQEPIENGLAKTGVYNIIGSTEVKNEAKISSQTQFTLEKGDKINYDQVLTADGYQWISYKSYSGVRRYIPVKKLT

TSSEKAKDEATKPTSYPNLPKTGTYTFTKTVDVKSQPKVSSPVEFNFQKGEKIHYDQVLVVDGHQWISYKSYSGIRR

YIEI

SeqID 320

MENWKFALSSILGHKMRAFLTMLGIIIGVASVVLIMALGKGMKDSVTNEITKSQKNLQIYYKTYEDQKNEDNFGAQG

AFMQGSDTNRKKPIIQESWLKKIAKEVDGVSGYYVTNQTNAPVAYLEKKAKTVNITGVNRTYLGIKKFKIKSGRQFQ

EEDYNQFSRVILLEEKLAQRLFQTNEAALNKVVTVKNKSYLVVGVYSDPEAGSGLYGSNSDGNAILTNTQLASEFGA

KEAENIYFHLNDVSQSNRIGKEIGKRLTDISHAKDGYYDNFDMTSIVKSINTQVGIMTGVIGAIAAISLLVGGIGVM

NIMLVSVTERTREIGLRKALGATRRKILAQFLIESMVLTILGGLIGLLLAYGGTMLIANAQDKITPSVSLNVAIGSL

IFSAFIGIIFGLLPANKASKLNPIDALRYE

SeqID 321

MQYSEIMIRYGELSTKKKNRMRFINKLKNNMEHVLSIYPDVSVKTDRDRGHVYLNGTDYHEVAESLKEIFGIQAFSP

SFKVEKNVDTLVKAVQEIMTSVYKDGMTFKITAKRSDHSFELDSRALNHTLGDAVFSVLPNIKAQMKQPDINLKVEI

RDEAAYISYENIRGAGGLPVGTSGKGMLMLSGGIDSPVAGYLALKRGVDIEAVHFASPPYTSPGALKKAHDLTRKLT

KFGGNIQFIEVPFTEIQEEIKEKAPEAYLMTLTRRFMMRITDRIRENRNGLVIINGESLGQVASQTLESMQAINAVT

ATPIIRPVVTMDKLEIIDIAQKIDTFDISIQPFEDCCTIFAPDRPKTNPKIKNTEQYEKRMDVEGLVERAVAGIMVT

TIQPQADSDDVDDLIDDLL

SeqID322

MARVKGGVVSRKRRKRVLKLAKGYYGAKHILFRTAKEQVMNSYYYAYRDRRQKKRDFRKLWITRINAAARMNGLSYS

QLMHGLKLAEIEVNRKMLADLAVNDAAAFTALADAAKAKLGK

SeqID 323

MFKASKKLVQKNKSNHFWLVFFITLILFLIGCYASLRFGAINFKTSDLITVLKNPLKNSNAQDVIFDIRLPRIIAAI

LVGAAMSQAGAIMQGVTRNAIADPGLLGINAGAGLALVVAYAFLGSMHYSTILIVCLLGSVISYLLVFTLSYTKQKG

YHQLRLILAGAMISTLFTSVGQVVTLYFKLNRTVIGWQAGGLSQINWKMLIIIAPIIILGLLISQLLAHQLTILSLN

ESVAKALGQKTQLMTAFLLLIVLFLSASSVALIGTVSFIGLIIPHFIKLFIPKDYRLLLPLIGFSGATFMIWVDLSS

RIINPPSETPISSIISIVGLPCFLWLIRKGKNL

SeqID 324

MIKRPIHLSHDFLAEVIDKEAITLDATMGNGNDTVFLAKSSKKVYAFDIQEEAIAKTKAKLTEQGISNAELILDGHE

NLEQYVHTPLRAAIFNLGYLPSADKTVITKPHTTIKAIKNVLDILEVGGRLSLMVYYGHDGGKSEKDAVIAFVEQLP

QNNFATMLYQPLNQVNTPPFLIMVEKLQSYENEV

SeqID 325

MRVHITSIYGQSPRSIALISQKLVKDVGRQLGYDEMGIYFYNDHAETHGERSTRMDGIIAGLGRGDIVVFQVPTWNS

TEFDELFLDKLQAYGARIITFVHDIVPLMFESNFYLLDRVIDMYNRSDVVILPTKAMHDYLIEKGMTTSKVLYQEVW

DHPVNIDLPRPECQKVLSFAGDIQRFPFVNDWKENIPLIYYGDGSRLNSEANVHAQGWKDDVELMLSLSKRGGFGLC

WSEDREELVERRYSRMNASYKLSTFLAAGLPIIANHDISSRDFIKQHGLGFTVETLEEAVEKINNMEKETYDSYVEN

VEKIATLLRNGYITKKLLIDAVHMLYR

SeqID 326

MSQKTFGKQLTVVDTKSRVKMHKSGKNWVRTVMSHFNLFKAIKGRATVEADVCIQDVEKEDRLSSGNLTYLKGILAA

GALVGGASLTSRVYADETPVVQEQSSSVPTLAEQTEVTVKTTTVQNHQDGTVSKNIIDSNSVSMSESASTSTSESVS

MSMSGSTLTSVSESVSTSALTSASESISTSASESVSKSTSISEVSNILETQASLTDKGRESFSANQIVTESSLVTDA

GKNASVSSLIEITKPKSELQTSKMSNESLITPEKSQVMIASDKTGNESLTPTIRLKSVIQPRSMNLMTLSSEMDLIP

LEEVSDTEMLGKDVSSELQKVNIALLDMTLSEPGTVKLDSSENLVLNFAFSIASVNEGDVFTVKLSDNLDTQGIGTI

LKVQDIMDETGQLLATGSYSPLTHNITYTWTRYASTLNNIKARVNMPVWPDQRIISKTTSDKQCFTATLNNQVASIE

ERVQYNSPSVTEHTNVKTNVRSRIMKLDDERQTETYITQINPEGKEMYFASGLGNLYTIIGSDGTSGSPVNLLNAEV

KILKTNSKNLTDSMDQNYDSPEFEDVTSQYSYTNDGSKITIDWKTNSISSTTSYVVLVKIPKQSGVLYSTVSDINQT

YGSKYSYGHTNISGDSDANAEIKLLSESASTSASTSASTSASMSASTSASTSASMSASTSASTSASMSASTSASTSA

STSTSTSASTSASTSASTSASMSASTSASTSASTSASTSASTSASTSASTSASTSASTSASTSASTSASTSASTSAS

TSASTSASMSASMSASTSASMSASTSASTSASTSASTSASTSASTSASMSASTSASTSASTSASMSASTSASTSAST

SASTSASTSTSTSASTSASTSASTSASMSASTSASTSPSTSASTSASTSASTSASTSASTSASMSASTSASTSASMS

ASTSASTSASTSASTSASMSASTSASTSASTSASMSASTSASTSASTSASTSASTSTSTSASTSASTSASTSASMSA

STSASTSASTSASMSASTSASISASTSASMSASTSASTSASTSASTSASMSASTSASTSASTSASTSASMSASTSAS

TSASTSASTSASTSASTSASTSASTSSSTSASTSASTSASTSASMSASTSASTSASMSASTSASTSASTSASMSAST

SSSTSASMSASTSASMSASTSASTSASTSASMSASTSSSTSASMSASTSASMSASTSASTSASTSASMSASTSASMS

ATTSASTSVSTSASTSASTSASTSSSSSVTSNSSKEKVYSALPSTGDQDYSVTATALGLGLMTGATLLGRKKSKKDK

D

SeqID 327

MIDRKDTNRFKLVSKYSPSGDQPQAIETLVDNIEGGEKAQILKGATGTGKTYTMSQVIAQVNKPTLVIAHNKTLAGQ

LYGEFKEFFPDNAVEYFVSYYDYYQPEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVVASVSCIYGLG

SPKEYADSVVSLRPGQEISRDQLLNNLVDIQFERNDIDFQRGKFRVRGDVVEVFPASRDEHAFRIEFFGDEIDRIRE

IESLTGRVLGEVEHLAIFPATHFMTNDEHMEEAISKIQAEMENQVELFEKEGKLIEAQRIRQRTEYDIEMLREMGYT

NGVENYSRHMDGRSEGEPPFTLLDFFPEDFLIMIDESHMTMGQIKGMYNGDRSRKEMLVNYGFRLPSALDNRPLRRE

EFESHVHQIVYVSATPGDYEMEQTDTVVEQIIRPTGLLDPEVEVRPSMGQMDDLLGEINLRTEKGERTFITTLTKRM

AEDLTDYLKEMGVKVKYMHSDIKTLERTEIIRDLRLGVFDVLIGINLLREGIDVPEVSLVAILDADKEGFLRNERGL

IQTIGRAARNSNGHVIMYADKITDSMQRAMDETARRRRLQMDYNEKHGIVPQTIKKEIRDLIAITKSNDSDKPEKVV

DYSSLSKKERQAEIKALQQQMQEAAELLDFELAAQIRDVILELKAID

SeqID 328

MKHKLKAFTLALLSIFFVFGGKVSAETVNIVSDTAYAPFEFKDSDQTYKGIDVDIVNEVAKRAGWNVNMTYPGFDAA

VNAVQSGQADALMAGTTVTEARKKVFNFSDTYYDTSVILYTKNNNKVTNYKQLKGKVVGVKNGTAAQSFLEENKSKY

GYKVKTFDTSDLMNNSLDSGSTYAAMDDQPVVQFAINQGKAYAINMEGEAVGSFAFAVKKGSGHDNLIKEFNTAFAQ

MKSDGTYNDIMDKWLGKDATKTSGKATGNANEKATPVKPSYKIVSDSSFAPFEYQNGKGKYTGFDMELIKKIAKQQG

FKLDISNPGFDAALNAVQSGQADGVIAGATITEARQKIFDFSDPYYTSSVILAVKKGSNVKSYQDLKGKTVGAKNGT

ASYTWLSDHADKYNYHVKAFDEASTMYDSMNSGSIDALMDDEAVLAYAINQGRKFETPIKGEKSGDIGFAVKKGANP

ELIKMFNNGLASLKKSGEYDKLVKKYLSTASTSSNDKAAKPVDESTILGLISNNYKQLLSGIGTTLSLTLISFAIAM

VIGIIFGMMSVSPSNTLRTISMIFVDIVRGIPLMIVAAFIFWGIPNLIESITGHQSPINDFVAATIALSLNGGAYIA

EIVRGGIEAVPSGQMEASRSLGISYGKTMQKVILPQAVRLMLPNFINQFVISLKDTTIVSAIGLVELFQTGKIIIAR

NYQSFRMYAILAIMYLVIITLLTRLAKRLEKRLK

SeqID 329

MGDKPISFRDKDGNFVSAADVWNAEKLEELFNTLNPNRKLRLEREKLAKEKG

SeqID 330

METYTLSNTLNIPKIGFGTWQLTEGEEAYKAYTHALKVGYTHTDTAQIYGNEHSVGRAIRDSGLARESIFLTTKIWN

DKHDYHLAKASIDESLQKLGVDYIDLLLIHWPNPKALRENDAWKAGNAGTWKAMEEAYKEGKVKAIGVSNFMKHHLE

ALFETAEIKPMVNQIILAPGCAQEDLVRFCKGNDILLEAYSPFGTGAIFENESIKAIAEKYGKSVAQVALRWSLDNG

FLPLPKSATPKNIEANLDIFDFQLNEDDITTLIQLDSGIKPKDPDNVSF

SeqID 331

MVKGQGNVVAQNKKAHHDYTIVETIEAGIVLTGTEIKSVRAARITLKDGYAQIKNGEAWLINVHITPYDQGNIWNQD

PDRTRKLLLKKREIEKISNELKGTGMTLVPLKVYLKDGFAKVLLGLAKGKHDYDKRESIKRREQNRDIARQLKNYVS

R

SeqID 332

MDHFTKLWQDFSKLPNVVAIALGGSRSGDSFDQSSDYDLYVYCAATPDITSRKRILNKHCHYIELNNHYWELEDNGT

LNDGTDIDILYRNIDNFLSDLEDVVEHHNSRIGYTTCFWHNLINCQILYDPENQLQSLKERFEVSYPSQLQKQIIIQ

NRNLLTGKLPSYDKQIIKALKRQDFVSTHHRTTAFLDSYFDIIFALNKLTHPGEKRMISYAKKNATLLPKHFEENII

KLCHHNSNEHTVKETLNDIIMHLDVMLKENFQHFIG

SeqID 333

MKKIIYLGLACVSILTLSGCESIERSLKGDRYVDQKLAENSSKEATEQLNKKTKQALKADKKAFPQLDKAVAKNEAQ

VLIKTSKGDINIKLFPKYAPLAVENFLTHAKEGYYNGLSFHRVIKDFMIQSGDPNGDGTGGKSIWNSKDKKKDSGNG

FVNEISPYLYNIRGSLAMANAGADTNGSQFFINQSQQDHSKQLSDKKVPKVIIKAYSEGGNPSLDGGYTVFGQVISG

METVDKIASVEVTKSDQPKEKITITSIKVIKDYKFK

SeqID 334

MKIGIIAAMEEELKLLVENLEDKSQETVLSNVYYSGRYGEHELVLVQSGVGKVMSAMSVAILVESFKVDAIINTGSA

GAVATGLNVGDVVVADTLVYHDVDLTAFGYDYGQMSMQPLYFHSDKTFVSTFEAVLSKEEMTSKVGLIATGDSFIAG

QEKIDVIKGHFPQVLAVEMFGAAIAQAAQATGKPFVVVRAMSDTAAHDANITFDEFIIEAGKRSAQVLMAFLKAL

SeqID 335

MEKRLSLGALVLASTVLLAACGNVGGGASSTGTKIGKDIKVGYNWELSGNVSSYGNSMLNGADLAVKEINAAGGVGG

KKLKVLSQDNKSENAEAATVATNLVTKGANVIIGPATSGAAASSTPKVNAAAVPMIAPAATQDNLVYGSDGKTLNQY

FFRATFVDNYQGKLLSQYATDNLKAKKVVLFYDNSSDYSKGVAKSFKESYSGKIVDSMTFSAGDTDFQASLTKLKGK

FYDAIVMPGYYTETGLIVLQARDLGISKPVLGPDGFDSPKFVQSATPVGASNVYYLTGFTTQGSTKAKAFHDHYYKA

YGEEPSMFSALSYDAVYMAAKSAKGAKTSIDLKKALAKLKDFKGVTGKMSIDKNHNVVKSAYVVKLDDGKTSSVNII

SAK

SeqID 336

MENHNSIKQTYGLMTTIAMIVGVVIGSGIYFKVDDILKFTGGDVFLGMVILVLGSFSIVFGSLSISELAIRTSESGG

IFSYYEKYVSPALAATLGLFASFLYLRTLTAIVSWVAAFYTLGESSSLESQIILAAVYILALSLMNIFAKRIAGGFQ

SLTTFVKMIPLVLIALIGAFWSDKAPQLPQHLTAIQPSNVGWSWVSGLVPLYFAYDGWTIFVSIAPEVKNPKKNLPL

AFVIGPALILLSYLAFFYGLTQILGASFIMTTGNDAINYAANIIFGPSVGRLLSFIVILSVLGVANGLLLGTMRLPQ

AFAERGWIKSERMANINLKYQMSLPASLTVTAVAIFWLFVHFMVTKFNLLPGSDISEIVVVFNNTSLIILYVLVLSL

YLKKDIKNKFTGLVSPILAILGGLILFIGSLLSNFFTVLIFQCFCLLFCLICHYTYQKNNPKTHE

SeqID 337

LTEFNDDQHSNHDQKSFKEQILAELEEANRLRKLREEELYQKEQEAKEAARRTAQLMADYEAQRLKDEQEARAKALE

TKQRLEEQEKARIEAKLLAEAAREEERRQAEQALASQEEQVINQGMEPSRELDSGSKSSEFRTTENVPDIDLKADKT

DVATAVPNQETEEIFLVRATDIPTEGENVKLGETSELEPVAKEPIRVEDLSKEEEDIALSAKNKHNKRERRQKADNV

AKRIARILISIIILVLLLTAFVGYRFVDSAIKPVDSNSNKFVQVEIPIGSGNKLIGQILEKAGVIKSATVFNYYSKF

KNYSNFQSGYYNLKKSMTLDQIAAELEKGGTAEPTKPALGKILITEGYTIKQIAKAIESNKIDTKTTSTPYKADDFL

KLVQDETFIKKMVAKYPNLLGSLPDKSKAIYQLEGYLFPATYNYYKDTTLEGLVEDMISTMNTKMAPYYNTIKAKNM

SVNDVLTLSSLVEKEGSTDEDRRKIASVFYNRLSAGQALQSNIAILYAMGKLGDKTSLAEDAQINTSIKSPYNIYTN

TGLMPGPVDSPSISAIEATIKPASTDYLYFVADVKTGNVYYAKDFETHKANVEKYINSQIN

SeqID 338

MSRMIPGRIRNQGIELYEQGLVSLISQEGNLLKAKVGDCQIEYSLVTEETKCSCDFFARKGYCQHLAALEHFLKNDP

EGKAILSKVQVQQESQQETKKKTSFGSVFLDSLIINEDDTIKYQLSAQGEQNPYANDIWWTLKIRRLPDDRSYVIRD

IKAFLNTVRKEAYYQIGKQYFETLSLIQFDETSQELIEFLWRLIPSHSSKIDLEFILPNQGRHLSLTRGFFEEGVTL

MNALENFSFESDFHQFNHLYFKELEGEDHLYQFKVIVHRQSIELEIKEKDLKPLFANSYLFYRDTFYHLNLKQEKMV

TAIRSLPIEGDLAKHIHFDLDDQDKLAAHLLDFKEIGLVDAPRSFSIHDFKVNFEFDINSQNEILLQMVFDYGNDLT

VHNRQELEQLTFASHFKHEEKIFKLLERYGFAPHFSTSHPAYSAQELYDFYTYMLPQFKKMGTVSLSAKLESYRLIE

RPQIDIEAKGSLLDISFDFSDLLENDVDQALVALFDNNPYFVNKSGQLVIFDEETKKVSATLQGLRARRAKNGHIEL

DNIAAFQLSELFANQDNVSFSQHFYQLIEDLRHPEKFKIPGLSVSASLRDYQLTGVRWLSMLDHYGFAGILADDMGL

GKTLQTISFLSTKLTRDSRVLILSPSSLIYNWQDEFHKFAPDVDVAVAYGSKIRRDEIIAERHQVIITSYSSFRQDF

ETYSEGNYDYLILDEAQVMKNAQTKIAHSLRSFEVKNCFALSGTPIENKLLEIWSIFQIILPGLLPGKKEFLKLNPK

QVARYIKPFVMRRRKEEVLPELPDLIEMNYPNEMTDSQKVIYLAQLRQIQESIQHSSDADLNRRKIEILSGITRLRQ

ICDTPRLFMDYDGESGKLESLHQLLTQIKENGHRALIFSQFRGMLDIAEREMVAMGLTTYKTTGSTPANERHEMTRA

FNAGSKDAFLISLKAGGVGLNLTGADTVVLIDLWWNPAVEMQAISRAHRLGQKENVEVYRLITRGTIEEKILEMQET

KKHLVTTVLDGNETHASMSVDDIREILGVSK

SeqID 339

VLLTEIKKSPEGLYFDKKIDIKESLMERHSEIMDISDIQVSGHVVYEDGLYLLDYNMAYDITLPSSRSMKPVVLSEK

QTINEVFIEAENVSTKKELVDQDLVLILEEDDINLEESVIDNILLNIPLRVLAADEVGVEADLSGKNWSLMTEKQYE

EKQAKEKEKSNPFAALEGMFDSD

SeqID 340

MEVKTAIEWMHTFNQKIQSNKDYLSELDTPIGDGDHGGNMARGMTAVIENLDNNEFSSAADVFKTVSMQLLSKVGGA

SGPLYGSAFMGITKAEQSESTISEALGAGLEMIQKRGKAELNEKTMVDVWHGVIEAIEKNELTEDRIDSLVDATKGM

KATKGRASYVGERSLGHIDPGSFSSGLLFKALLEVGGV

SeqID 341

MQDKLMIRGARAHNLKNISVDIPRDKLVVVTGLSGSGKSSLAFDTIYAEGQRRYVESLSAYARQFLGNMEKPDVDSI

DGLSPAISIDQKTTSKNPRSTVGTVTEINDYLRLLYARVGTPYCINGHGAITASSVEQIVDKVLALPERTKMQILAP

IIRRKKGQHKSTFEKIQKDGYVRVRIDGDIHDVTEVPELSKSKMHNIDIVVDRLINKEGIRSRLFDSVEAALRLSDG

YVVIDTMDGNELLFSEHYSCPECGFTVPELEPRLFSFNAPFGSCTTCDGLGIKLEVDIDLVIPDKSKTLREGALVPW

NPISSNYYPTMLEQAMTQFDVDMDTPFEKLSKAEQDLALYGSGEREFHFHYINDFGGERNIDLPFEGVVNNINRRYH

ETNSDYTRNVMREYMNELKCNTCHGYRLNDQALCVRVGGEEGLNIGQVSDLSIADHLELLETLRLSSNEQLIARPII

KEIHDRLSFLNNVGLNYLNLSRSAGTLSGGESQRIRLATQIGSNLSGVLYVLDEPSIGLHQRDNDRLIDSLKKMRDL

GNTLIVVEHDEDTMMAADWLIDVGPGAGAFGGEIVASGTPKQVAKNTKSITGQYLSGKKVIPVPSERRVGNGRFLEI

KGAAENNLQNLDVKFPLGKFIAVTGVSGSGKSTLINSILKKAVAQKLNRNSDKPGKYVSLEGIEYVDRLIDIDQSPI

GRTPRSNPATYTGVFDDIRDLFAQTNEAKIRGYKKGRFSFNVKGGRCESCSGDGIIKIEMHFLPDVYVPCEVCHGTR

YNSETLEVHYKEKNIAQILDMTVNDAVTFFAAIPKIARKLQTIKDVGLGYVTLGQPATTLSGGEAQRMKLASELHKR

STGKSLYILDEPTTGLHADDIARLLKVLDRFVDDGNTVLVIEHNLDVIKTADHIIDLGPEGGIGGGQIVAIGTPEEV

AENPKSYTGYYLKEKLAR

SeqID 342

MWPEDRIASFRRTLLGWYDQEKRDLPWRRTTNPYYIWVSEIMLQQTQVNTIPPYYKRFLKWFPQIKDLADAPEEQLI

KAWEGLGYYSRVRNMQKAAQQVMVDFGGIFPHTYDDIASLKGIGPYTAGAIASISFNLPEPAVDGNVMRVMARLFEV

NYDIGDPKNRKIFQAIMEILIDPDRPGDFNQALMDLGTDIESAKTRPPDESPIRFFNAAYLNGTYSKYPIKNPKKKP

KPMRIQAFVIRNQNGQYLLEKNTKGRLLGGFWSFPIIETSPLSQQLDLFDDNQSNPIIWQTQNETFQREYQLKPQWT

DNHFPNIKHTFSHQKWTIELIEGVVKATDLPNAPHLKWAAIEDFSLYPFATPQKKMLETYLKQKNA

SeqID 343

LIEITWTVKYITEFIATAFLIILGNGAVANVDLKGTKGNNSGWIIIAIGYGLGVMMPALMFGNVSGNHINPAFTLGL

AFSGLFPWAHVGQYILAQILGAMFGQLVVVMVYQPYFVKTENPNHVLGSFSTISALDDGQKSSPKAAYINGFLNEFV

GSFVLFFGALALTKNYFGVELVGKLVQAGYDQTTAATRISPYVTGSLAVAHLGIGFLVMTLVASLGGPTGPALNPAR

DLGPRIVHRLLPKQILGQAKEDSKWWYAWVPVLAPIVASILAVALFKLLYL

SeqID 344

MSSYWNNYPELKKNIDETNQLIQERIQVRNKDIEAALSQLTAAGGKQLRPAFFYLFSQLGNKENQDTQQLKKIAASL

EILHVATLIHDDVIDDSPLRRGNMTIQSKFGKDIAVYTGDLLFTVFFDLILESMADTPFMRINAKSMRKILMGELDQ

MHLRYNQQQGIHHYLRAISGKTAELFKLASKEGAYFGGAEKEVVRLAGHIGFNIGMTFQILDDILDYTADKKTFNKP

VLEDLTQGVYSLPLLLAIEENPDIFKPILDKKTDMATEDMEKIAYLVVSHRGVDKARHLARKFTEKAISDINKLPQN

SAKKQLLQLTNYLLKRKI

SeqID 345

MFKIPLFKELKTDQWVKPFFKQYKVSLVIALFFLFMTFFSASALMFNSGYLISKSASLPSNILLVYVPIVLTRAFGI

GRPVFRRYIERLTSHNWVLMTSQLRLKLYHSLESNAIFMKRDFRLGDVMGLLAEDINYLQNLYLRTIFPTIIAWLLY

SFIIIATGFFSLWFALMMLLYLAIMIFLFPLWSILANGARQTREKELKNHLYTDLTDNVLGISDWIFSQRGQEYVAL

HERSESELMAIQKKIRSFNNRRALIVELVFGFLAILVITWASNQFIGHRGGEANWIAAFVLTVFPLSEAFAGLSAAA

QETNKYSDSIHRLNELSETYFETTQNQLPNKPYDFSVKNLSFQYKPQEKWVLHHLDLDIKEGEKIAILGRSGSGKST

LASLLRGDLKASQGKITLGGADVSIVGDCISNYIGVIQQAPYLFNTTLLNNIRIGNQDASEEDVWKVLERVGLKEMV

TDLSDGLYTMVDEAGLRFSGGERHRIALARILLKDVPIVILDEPTVGLDPITEQALLRVFMKELEGKTLVWITHHLK

GIEHADRILFIENGQLELEGSPQELSQSSQRYRQLKAADDGDL

SeqID 346

MKKLITEKKVNNVVSTVNYLKLLVSAMFAGGAFVALGSTQGVSASTFTAPQATHPKAERQLTDSEIYERAQKQVLPK

YIQGSLSGILNQHSTLYKQNAAVTPQVSSPKAERQLTDSEIYERAQKQVLPKYIQGSLSGILNQHSTLNA

SeqID 347

MEENMNIKQLKSKTMLGTVALVSAFSFASTNADANTYNYAVDVDYLASAEEIAQAHPASNTFPLGQCTWGVKEMATW

AGNWWGNGGDWAASAASAGYTVGTQPRVGSIVCWTDGSYGHVAYVTAVDPVTNKIQVLESNYAGHQWIDNYRGWFDP

QNTATPGVVSYIYPN

SeqID 348

MKINQMKKDELFEGFYLIKKAEVRKTRAGKDFIAFTFRDDTGEISGNMWDAQTYNVEEFVAGKIVHMKGRREVYNGT

PQVNQITLRNIKDGEPNDPRDFKEKPPINVDNVREYMEQMLFKIENATWQRVVRALYRKYNKEFFTYPAAKTNHHAF

ESGLAYHTATMVRLADSIGDIYPELNKSLMFAGIMLHDLAKVIELSGPDNTEYTIRGNLIGHISLIDEELTKILAEL

NIDDTKEEVTVLRHVILSHHGQLEYGSPVRPRIMEAEIIHMIDNIDANMMMMTTALNRVNEGEMTNRIFAMDNRSFY

KPNIK

SeqID 349

MLVDKKWRFEDSASYFACPKCQNPLIKESNSLKCSDNHCFDLSKFGYVNLLGGKKIDEHYDKKSFENRQLVLENGYY

NHILEAISKVLENNSQFHSVLDIGCGEGFYSRQLVNKYEKTFLAFDISKDSIQLAAKSDQSRLVKWFVSDLANLPIQ

DSSIDIILDIFSPANYKEFRRVLSDDGILVKVVPVAEHVQELREKASQYLKQKDYSNQKILDHFRENFEIISEQKVV

QSYNCSQQERQAFIDMTPLLFSVDRTTIDWASISEITVGALIVIGKKRSVSK

SeqID 350

MCLICQRIEMIERNENPYFVKEYETGYLVLGDHQYFQGYCLFLSKKHVTELHELPRDWRNQYLSEMADASEIVAKAF

RADKMNIESLGNGDAHLHFHLFPRKTGDLRNYGHNGKGPVWWYPFEKMYADSVRATGAEIEKLKEKLLDVLG

SeqID 351

MFSDLRKKFVFLTMSILIVVVLFLFAVSNRYNQYWDEYDAYRIVKLVAKNDYLGIPGDEPIALVTIDNQKMVKIQSN

NTDLTNDVIEKSSLKLLEQGKKSRKWKSFIYSIKEYKDKTYTIAIMDLASYEVPYARRFLILVFTIFGFCLLAAVSL

YLSRFIVSPVETEMTREKQFVSDASHELKTPIAAIRANVQVLEQQIPGNRYLDHVVSETKRMEFLIEDLLNLSRLDE

KRSKVNKKKLNLSVLCQEVLLTYESLAYEEEKCLNDTIEDDVWIVGEESQIKQILIILLDNAIRHSLSKSEIQFSLK

QARRKAILTISNPSAIYSKEVMDNLFERFYQAKDDHADSLSFGLGLSIAKAIVERHKGRIRAYQEKDQLRLEVQLPI

DGFWTNTMIN

SeqID 352

LIILDKKSYDLLFYLLKLEEPETVMAIANALNQSRRKVYHLEKKINDALPSDVPQIVSYPRVGILLTEKQKAACRLL

LDEVTDYSYVMKSSERLQLSLVSIVVAKDRITIDRLMQLNDVSRNTILNDLNELRSELAEKEYNLQLQSTKCRGYFL

DGHPLSIIQYLYKLLDDIYHYGSSSFIDLFNHKLSQAFGASTYFSKEVLDYFHHYLFISQRSLGKKINSQDGQFMIQ

ILPFILMAYRKNRLSPEVQTSLNSDFSLVWQRKEYEIAKELADELEENFQLSLDEIEVGLVAMLMLSFRKDRDNHLE

SQDYDDMRATLTSFLKELEERYHLHFVHKKDLLRQLLTHCKALLYRKRYGIFSVNPLTEHIKDKYELLFAITSSSVK

LLEKAWQIKLTDDDVAYLTIHLGGELRNSQQSPNKLKLVIVSDEGIAIQKLLLKQCQRYLTNSDIEAVFTTEQYQSV

SDLMHVDMVVSTSDALESRFPMLVVHPVLTDDDIIRLIRFSKKGNCANSNQFTNELELTIAQYVKEDSERYVLKSKI

EKLIHQELLQDVLPLQSTVC

SeqID 353

MKYFLDTADVSEIRRLNRLGIVDGVTTNPTIISREGRDFKEVINEICQIVDGPVSAEVTGLTCDEMVTEAREIAKWS

PNVVVKIPMTEEGLAAVSQLSKEGIKTNVTLIFTVAQGLSAMKAGATFISPFVGRLEDIGTDAYALIRDLRHIIDFY

GFQSEIIAASIRGLAHVEGVAKCGAHIATIPDKTFASLFTHPLTDKGIETFLKDWDSFKKK

SeqID 354

MSHYSIKLQEVFRLAQFQAARYESHYLESWHLLLAMVLVHDSVAGLTFAEYESEVAIEEYEAATILALGRAPKEEIT

NYQFLEQSPALKKILKLAENISIVVGAEDVGTEHVLLAMLVNKDLLATRILELVGFRGQDDGESVRMVDLRKALERH

AGFTKDDIKAIYELRNPKKAKSGASFSDMMKPPSTAGDLADFTRDLSQMAVDGEIEPVIGRDKEISRMVQVLSRKTK

NNPVLVGDAGVGKTALAYGLAQRIANGNIPYELRDMRVLELDMMSVVAGTRFRGDFEERMNQIIADIEEDGHIILFI

DELHTIMGSGSGIDSTLDAANILKPALARGTLRTVGATTQEEYQKHIEKDAALSRRFAKVLVEEPNLEDAYEILLGL

PKAYEAFHNVTISDEAVMTAVKVAHRYLTSKNLPDSAIDLLDEASATVQMMIKKNAPSLLTEVDQAILDDDMKSASK

ALKASYKGKKRKPIAVTEDHIMATLSRLSGIPVEKLTQADSKKYLNLEKELHKRVIGQDDAVTAISRAIRRNQSGIR

TGKRPIGSFMFLGPTGVGKTELAKALAEVLFDDESALIRFDMSEYMEKFAASHLNGAPPGYVGYDEGGELTEKVRNK

PYSVLLFDEVEKAHPDIFNVLLQVLDDGVLTDSRGRKVDFSNTIIIMTSNLGATALRDDKTVGFGAKDISHDYTAMQ

KRIMEELKKAYRPEFINRIDEKVVFHSLSQDNMREVVKIMVKPLILALKDKGMDLKFQPSALKHLAEDGYDIEMGAR

PLRRTIQTQVEDHLSELLLANQVKEGQVIKIGVSKGKLKFDIAKS

SeqID 355

MVLDKEIKAQLAQYLDLLESDIVLQADLGDNDNSQKVKDFLDEIVAMSDRISLESTHLKRQPSFGIAKKGHESRVIF

SGLPMGHEFTSFILALLQVSGRAPKVDEDIIKRIKGIEKTINLETYVSLTCHNCPDVVQAFNIMAVLNPNITHTMIE

GGMYQDEVKSKGIMSVPTVYKDQEEFTSGRATIEQLLEQLDGPLDAEAFADKGVYDVLVIGGGPAGNSAAIYAARKG

LKTGILAETFGGQVIETVGIENMIGTLYTEGPKLMAQIEEHTKSYDIDIIKSQLATGIEKKELVEVTLANGAILQAK

TAILALGAKWRNINVPGEEEFRNKGVTYCPHCDGPLFEGKDVAVIGGGNSGMEAALDLAGVTKHVTVLEFLPELKAD

QVLQERAAKTDNLTILKNVATKDIVGEDHVTGLNYTDRDTNEEKHIDLEGVFVQIGLVPSTSWLKDSGIELNERQEI

VVDKFGSTNIPGIFAAGDCTDAAYKQIIISMGSGATAAIGAFDYLIRQ

SeqID 356

MIKYQDDFYQAVNGEWAKTAVIPDDKPRTGGFSDLADDIEALMLSTTPKWLADENKPSDTILNHFIAFHKMTADYQK

REEVGVSPVLPLIEEYKGLQSFSEFASKVAEYELEGKPNEFPFGVAPDFMNAQLNVLWAEAPGIILPDTTYYSEDNE

KGKELLAFWRKSQEDLLPLFGLSEQEIKDILDKVLALDAKLAQYVLSREESSEYVKLYHPYNWEDFTKLAPELPLDA

IFQKILGQKPDKVIVPEERFWTEFASDYYSESNWELLKADLILSAANAYNAYLTDDIRIKSGVYSRALSGTPQAMDK

KKAAYYLASGPYNQALGLWYAGEKFSPEAKADVEHKIATMIDVYKSRLEKADWLAQSTREKAIMKLNVITPHIGYPE

KLPETYTKKIIDPKLSVLENATNLDKISIAYGWSKWNKKPDRSEWHMPAHMVNAYYDPQQNQIVFPAAILQEPFYAL

EQSSSANYGGIGAVIAHEISHAFDTNGASFDEHGSLNNWWTDEDFEAFKKLTDKVVEQFDGLESYGAKVNGKLTVSE

NVADLGGVACALEAAQRESDFSARDFFINFATIWRNKARDEYMQMLASVDVHAPAQWRTNITVTNFEEFHKEFDVKD

GDNMWRPVEKRVIIW

SeqID 357

MSKILFFGEPLIRITPKENDYFADSISTKLFYGGSEVNTARALQGFGQDTKLLSALPNNPIGNSFLQFLKAQGIDTH

SIQWVGERVGLYFLEDSFACRKGEVVYDRDHSSLHDFRINQIDFDQLFEGVSLFHFSGITLSLDESIQEITLLLLKE

AKKREITISLDLNFRSKLISPKNAKILFSQFATFADICFGIEPLMVDSQDTTFFNRDEATIEDVKERMISLINHFDF

QVIFHTKRLQDEWGRNHYQAYIANRKQEFVTSKEITTAVNQRIGSGDAFVAGALYQLLQHSDSKTVIDFAVASASLK

CALEGDNMFETVTAVNKVLNQSKDIIR

SeqID 358

MKSRKKDKLVLRLTTTLLVFGLGGVWFYNYKNDNVEPTVTSASDQTTTFIQTISPTAIEISKTYDLYASVLLAQAIL

ESSSGQSDLSKAPNYNLFGIKGEYKGKSVQMPTLEDDGKGNMTQIQAPFRAYPNYSASLYDYAELVSSQKYASVWKS

NTSSYKDATAALTGLYATDTAYASKLNQIIETYSLDAYDK

SeqID 359

MADVKVVNNEDSRGQKQDLKAKLFHIKIGSVPLPVYVCLALLLLLAGFLQKLPVNMLGGFAVILTMGWFLGTIGASI

PGFKNFGGPAILSLLVPSILVFFNLINKNVLESTNMLMKQANFLYFYIACLVSGSILGMNRKMLIQGLLRMIFPMLL

GMVCAMMVGTFVGVILGLEWRHTLFYIVTPVLAGGIGEGILPLSLGYSSITGVASEQLVAQLIPATIIGNFFAILCT

ALLNRLGEKKPHLSGQGQLVRLNKGEDMSDIIADHSGPIDVKKMGGGVLTACSLFIFGHLLQQLTGFPGPVLMIVAA

AILKYINVIPRETQNGAKQLYKFISGNFTFPLMAGLGLLYIPLKDVVATLSIQYFIVVISVVFTVISVGFFVSRFLN

MNPVEAGIISACQSGMGGTGDVAILSTADRMNLMPFAQVATRLGGAITVITMTAILRMLF

SeqID 360

RGKKAGKYTTSDGYIFDAKDIKKDTGTGYVIPHMTHEHWVPKKDLSESELKAAQEFLSGKSEANQDKPKTGKTAQEI

YEAIEPKAIVKPEDLLFGIAQATDYKNGTFVIPHKDHYHYVELKWFDEEKDLLADSDKTYSLEDYLATAKYYMMHPE

KRPKVEGWGKDAEIYKEKDSNKADKPSPAPTDNKSTSNSSDKNLSAAEVFKQAKPEKIVPLDKIAAHMAYAVGFEDD

QLIVPHHDHYHNVPMAWFDKGGLWKAPEGYTLQQLFSTIKYYMEHPNELPKEKGWGHDSDHNKGSNKDNKAKNYAPD

EEPEDSGKVTHNYGFYDVNKGSDEEEPEKQEDESELDEYELGMAQNAKKYGMDRQSFEKQLIQLSNKYSVSFESFNY

INGSQVQVTKKDGSKVLVDIKTLTEVK

SeqID 361

MNRKKTVIISALSVALFGTGVGAYQLGSYNAQKSDNSVSYVKTDKSDSKAQATAVNKTPDQISKEEGISAEQIVVKI

TDDGYVTSHGDHYHYYNGKVPYDAIISEELIMRDPSYVFNKADVINEVKDGYIIKVNGKYYLYLKEGSKRTNVRTKE

QIQKQREEWSKGGSKGESGKHSSAKTQALSASVREAKASGRYTTDDGYVFSPTDVIDDMGDAFLVPHGDHFHYIPKA

DLSPSELSAAQAYWNRKTGRSGNSSKPSNSSSYIHASAPSGNVSTGRHANAPISIPRVTHANHWSKPAGNHATAPKH

HAPTTKPINKDSALDKMLKRLYAQPLYARHVESDGLVYDPAQVNAFTAIGVSIPHGNHFHFIHYKDMSPLELEATRM

VAEHRGHHIDALGKKDSTEKPKKHISHEPNKEPHTEEEHHAVTPKDQRKGKPNSQIVYSAQEIEEAKLVNTQHLMVT

FLMPKILKKIQVQVMSFHI

SeqID 362

MAKFGFLSVLEEELDKHLQYDFAMDWDKKNHTVEVTFILEAQNSSAIETVDDQGETSSEDIVFEDYVLFYNPVKSRF

DAEDYLVTIPYEPKKGLSREFLAYFAETLNEVATEGLSDLMDFLTDDSIEEFGLSWDTDAFENGRAELKETEFYPYP

RY

SeqID 363

MNTKQRFSIRKYKLGAVSVLLGTLFFLGGITNVAADSVINKPSDIAVEQQVKDSPTSIANETPTNNTSSALASTAQD

NLVTKANNSPTETQPVAESHSQATETFSPVANQPVESTQEVSKTPLTKQNLAVKSTPAISKETPQNIDSNKIITVPK

VWNTGYKGEGTVVAIIDSGLDINHDALQLNDSTKAKYQNEQQMNAAKAKAGINYGKWYNNKVIFGHNYVDVNTELKE

VKSTSHGMHVTSIATANPSKKDTNELIYGVAPEAQVMFMRVFSDEKRGTGPALYVKAIEDAVKLGADSINLSLGGAN

GSLVNADDRLIKAIEMARLAGVSVVIAAGNDGTFGSGASKPSALYPDYGLVGSPSTAREAISVASYNNTTLVNKVFN

IIGLENNRNLNNGLAAYADPKVSDKTFEVGKQYDYVFVGKGNDNDYKDKTLNGKIALIERGDITFTKKVVNAINHGA

VGAIIFNNKAGEANLTMSLDPEASAIPAIFTQKEFGDVLAKNNYKIVFNNIKNKQANPNAGVLSDFSSWGLTADGQL

KPDLSAPGGSIYAAINDNEYDMMSGTSMASPHVAGATALVKQYLLKEHPELKKGDIERTVKYLLMSTAKAHLNKDTG

AYTSPRQQGAGIIDVAAAVQTGLYLTGGENNYGSVTLGNIKDKISFDVTVHNINKVAKDLHYTTYLNTDQVKDGFVT

LAPQQLGTFTGKTIRIEPGQTQTITIDIDVSKYHDMLKKVMPNGYFLEGYVRFTDPVDGGEVLSIPYVGFKGEFQNL

EVLEKSIYKLVANKEKGFYFQPKQTNEVPGSEDYTALMTTSSEPIYSTDGTSPIQLKALGSYKSIDGKWILQLDQKG

QPHLAISPNDDQNQDAVAVKGVFLRNFNNLRAKVYRADDVNLQKPLWVSAPQAGDKNYYSGNTENPKSTFLYDTEWK

GTTTDGIPLEDGKYKYVLTYYSDVPGSKPQQMVFDITLDRQAPTLTTATYDKDRRIFKARPAVEHGESGIFREQVCY

LKKDKDGHYNSVLRQQGEDGILVEDNKVFIKQEKNGSFILPKEVNDFSHVYYTVEDYASNLVSAKLEDLINIGNKNG

LVNVKVFSPELNSNVDIDFSYSVKDDKGNIIKKQHHGKDLNLLKLPFGTYTFDLFLYDEERNLLISPKSVTVTISEK

DSLKDVLFKVNLLKKAALLVEFDKLLPKGATVQLVTKTNTVVDLPKATYSPTDYGKNIPVGDYRNLVTLPSGYSTLE

NLDDLLVSVKEDQVNLTKLTLINKAPLINALAEQTDIITQPVFYNAGTHLKNNYLANLEKAQTLIKNRVEQTSIDNA

IAALRESRQALNGKETDTSLLAKAILAETEIKGNYQFVNASPLSQSTYINQVQLAKNLLQKPNVTQSEVDKALENLD

IAKNQLNGHETDYSGLHHMIIKANVLKQTSSKYQNASQFAKENYNNLIKKAELLLSNRQATQAQVEELLNQIKATEQ

ELDGRDRVSSAENYSQSLNDNDSLNTTPINPPNQPQALIFKKGMTKESEVAQKRVLGVTSQTDNQKVKTNKLPKTGE

STPKITYTILLFSLSMLGLATIKLKSIKRE

SeqID 364

MNNNEKKVKYFLRKTAYGLASMSAAFAVCSGIVHADTSSGISASIPHKKQVNLGAVTLKNLISKYRGNDKAIAILLS

RVNDFNRASQDTLPQLINSTEAEIRNILYQGQIGKQNKPSVTTHAKVSDQELGKQSRRSQDIIKSLGFLSSDQKDIL

VKSISSSKDSQLILKFVTQATQLNNAESTKAKQMAQNDVALIKNISPEVLEEYKEKIQRASTKSQVDEFVAEAKKVV

NSNKETLVNQANGKKQEIAKLENLSNDEMLRYNTAIDNVVKQYNEGKLNITAAMNALNSIKQAAQEVAQKNLQKQYA

KKIERISSKGLALSKKAKEIYEKHKSILPTPGYYADSVGTYLNRFRDKQTFGNRSVWTGQSGLDEAKKMLDEVKKLL

KELQDLTRGTKEDKKPDVKPEAKPEAKPNIQVPKQAPTEAAKPALSPEALTRLTTWYNQAKDLLKDDQVKDKYVDIL

AVQKAVDQAYDHVEEGKFITTDQANQLANKLRDALQSLELKDKKVAKPEAKPEAKPEAKPEAKPEAKPEAKPEAKPE

AKPDVKPEAKPDVKPEAKPEAKPEAKSEAKPEAKLEAKPEAKPATKKSVNTSGNLAAKKAIENKKYSKKLPSTGEAA

SPLLAIVSLIVMLSAGLITIVLKHKKN

SeqID 365

MAKDIKFSADARSAMVRGVDILADTVKVTLGPKGRNVVLEKAFGSPLITNDGVTIAKEIELEDHFENMGAKLVSEVA

SKTNDIAGDGTTTATVLTQAIVREGLKNVTAGANPIGIRRGIETAVSAAVEELKEIAQPVSGKEAIAQVAAVSSRSE

KVGEYISEAMGRVGNDGVITIEESRGMETELEVVEGMQFDRGYLSQYMVTDNEKMVSELENPYILITDKKISNIQEI

LPLLEEVLKTNRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKAPGFGDRRKAMLEDIAILTGGTVVTEDLGLDLK

DATMQVLGQSAKVTVDKDSTVIVEGAGDSSAIANRVAIIKSQMEATTSDFDREKLQERLAKLAGGVAVIKVGAATET

ELKEMKLRIEDALNATRAAVEEGIVSGGGTALVNVIEKVAALKLNGDEETGRNIVLRALEEPVRQIAYNAGYEGSVI

IERLKQSEIGTGFNAANGEWVDMVTTGIIDPVKVTRSALQNAASVASLILTTEAVVANKPEPEAPTAPAMDPSMMGG

F

Seq ID 366

VVENLEKPIGVSYKNSPSMSKRTAIRMKKSSRFSILLYSVLSTLLAIANPLLTYFANGLQTQNLYTGLMMTKGQIPY

SDVFATGGFLYYVTIALSYLLGSSIWLLIVQFIAYYVSGIYFYKLVYYVAQSEIVSIGMTLIFYIMNIVLGFGGMYP

IQWALPFMLISLWFLIKFCVDNIVDEAFIFYGILAAFSLFIDPQTLIFWLCSFVLLTATNIKQKQSLRGFYQFLCVV

FGMILIAYTVGYFMFNLQIISSYIDKAIFYPFTYFARTNHSFLLSLAIQIVVLLGSGCLFGLWDFIQNRKKASYQIG

LNFIACIFIIYAIMAIFSRDFNLYHFLPALPFGLLLTSNKITILYQKVIDRRSHRRQYFSGKSLIVDLFVKKTYYLP

LLLVSLSIGLLVYNTYQNVTLSKERRDISHYLTTKIDRDGKIYVWDKVASIYSQTRLKSASQFVLPHINTAQKNNEK

ILKDELLQHGAKYFILNKNEKLPNELKSDIKKHYQEVPLSNITHFVLYRFK

SeqID 367

MAKPTISPGMQQYLDIKENYPDAFLLFRMGDFYELFYDDAVKAAQILEISLTSRNKNAEKPIPMAGVPYHSAQQYID

VLVELGYKVAIAEQMEDPKKAVGVVKREVVQVVTPGTVVESTKPDSANNFLVAIDSQDQQTFGLAYMDVSTGEFQAT

LLTDFESVRSEILNLKAREIVVVYQLTDEKNHLLTKQMNLLLSYEDERLNDIHLIDEQLTDLEISAAEKLLQYVHRT

QKRELSHLQKVVHYEIKDYLQMSYATKNSLDLLENARTSKKHGSLYWLLDETKTAMGTRMLRTWIDRPLVSMNRIKE

RQDIIQVFLDYFFERNDLTESLKGVYDIERLASRVSFGKANPKDLLQLGQTLSQIPRIKMILQSFNQPELDIIVNKI

DTMPELESLINTAIAPEAQATITEGNIIKSGFDKQLDNYRTVMREGTGWIADIEAKERAASGIGTLKIDYNKKDGYY

FHVTNSNLSLVPEHFFRKATLKNSERYGTAELAKIEGEMLEAREQSSNLEYDIFMRVRAQVESYIKRLQELAKTIAT

VDVLQSLAVVAENYHYVRPKFNDQHQIKIKNGRHATVEKVMGVQEYIPNSIYFDSQTDIQLITGPNMSGKSTYMRQL

ALTVIMAQMGGFVSADEVDLPVFDAIFTRIGAADDLISGQSTFMVEMMEANQAVKRASDKSLILFDELGRGTATYDG

MALAQSIIEYIHDRVRAKTMFATHYHELTDLSEQLTRLVNVHVATLERDGEVTFLHKIESGPADKSYGIHVAKIAGL

PIDLLDRATDILSQLEADAVQLIVSPSQEAVTADLNEELDSEKQQGQLSLFEEPSNAGRVIEELEAIDIMNLTPMQA

MNAIFDLKKLL

SeqID 368

MLKLDLKTKIKEAILIAFGVALYTFGFVKFNMANHLAEGGISGVTLIIHALFGVNPALSSLLLNIPLFILGARILGK

KSLLLTIYGTVLMSFFMWFWQQIPVTVPLKNDMMLVAVAAGILAGTGSGLVFRYGATTGGADIIGRIVEEKSGIKLG

QTLLFIDAIVLTSSLVYINLQQMLYTLVASFVFSQVLTNVENGGYTVRGMIIITKESESAAATILHEINRGVTFLRG

QGAYSGREHDVLYVALNPSEVRDVKEIMADLDPDAFISVINVDEVISSDFKIRRRNYDK

SeqID 369

MKRSMYAGRVRSEHIGTSITLKGWVGRRRDLGGLIFIDLRDREGIMQLVINPEEVAASVMATAESLRSEFVIEVSGV

VTAREQANDNLPTGEVELKVQELSVLNTSKTTPFEIKDGIEANDDTRMRYRYLDLRRPEMLENFKLRAKVTHSIRNY

LDNLEFIDVETPMLTKSTPEGARDYLVPSRVNQGHFYALPQSPQITKQLLMNAGFDRYYQIVKCFRDEDLRGDRQPE

FTQVDLETSFLSDQEIQDIVEGMIAKVMKDTKGLEVSLPFPRMAYDDAMNNYGSDKPDTRFDMLLQDLTEIVKEVDF

KVFSEASVVKAIVVKDKADKYSRKNIDKLTEIAKQYGAKGLAWLKYVDNTISGPVAKFLTAIEGRLTEALQLENNDL

ILFVADSLEVANETLGALRTRIAKELELIDYSKFNFLWVVDWPMFEWSEEEGRYMSAHHPFTLPTAETAHELEGDLA

KVRAVAYDIVLNGYELGGGSLRENQKDTQERMFKALGFSAESAQEQFGFLLEAMDYGFPPHGGLAIGLDRFVMLLAG

KDNIREVIAFPKNNKASDPMTQAPSLVSEQQLEELSLTVESYEN

SeqID 370

KEKGKLIKKKILENNTDIIPKIIEKSPQNLILTSNYNRVNIDKIKNIKNFDKGFELGFPLFEKGEILRKEGEITSAI

ELFDKARELGYFVPALYNSYAMAFRKIKNYDDELLILQEGIERFKKSTLSSNINPKTIDRWSTRISRAKDLKCK

SeqID 371

LNVKKHHLAYGAITLVALFSCILAVMVIFKSSQVTTESLSKADKVRVAKKSKMTKATSKSKVEDVKQAPKPSQASNE

APKSSSQSTEANSQQQVTASEEAAVEQAVVTENTPATSQAQQAYAVTETTYRPAQHQTSGQVLSNGNTAGAIGSAAA

AQMAAATGVPQSTWEHIIARESNGNPNVANASGASGLFQTMPGWGSTATVQDQVNSAIKAYRAQGLSAWGY

SeqID 372

MSNWDTKFLKKGFTFDDVLLIPAESHVLPNEVDMKTKLADNLTLNIPIITAAMDTVTDSKMAIAIARAGGLGIIHKN

MSIVDQAEEVRKVKRSENGVIIDPFFLTPDNTVSEAEELMQNYRISGVPIVETLENRKLVGIITNRDMRFISDYKQL

ISEHMTSQNLVTAPIGTDLETAERILHEHRIEKLPLVDDEGRLSGLITIKDIEKVIEFPKAAKDEFGRLLVAGAVGV

TSDTFERAEALFEAGADAIVIDTAHGHSAGVLRKIAEIRAHFPNRTLLAGNIATAEGARALYDAGVDVVKVGIGPGS

ICTTRVVAGVGVPQITAIYDAAAVAREYGKTIIADGGIKYSGDIVKALAAGGNAVMLGSMFAGTDEAPGETEIFQGR

KFKTYRGMGSIAAMKKGSSDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIVFQMLGGIRSGMGYVGAANIKELHDN

AQFVEMSGAGLKESHPHDVQITNEAPNYSVH

SeqID 373

MTINHYKLRIPYYTISFLLPFIIIVCILFTKNIYWGSPTTILASDGFHQYVIFNQALRNILHGSNSLFYTFTSGLGL

NFYALSSYYLGSFLSPIVYFFNLKNMPDAIYLLTICKIGLIGLSMFVTLCKRHCKVNRVLLLVISTCYSLMSFSISQ

IEINMWLDVFILIPLVVLGVDQLLWERKPILYFLSLTALFIQNYYFGFMTAIFTSLYFIVQITRNTDSKVAFKQFLH

FTFLSLLAGMTSSIMILPTYFDLTTHGEKLTKVSKMFTRNSWYMDLFAKNMIGAYDTTKFGSIPMIYVGLLPLLLSL

LYFTIKEVPRRTRLAYGFLIIFVIASFYITPLDLFWQGMHAPNMFLHRYSWVLSVLICLLAAECLEYLDNISWKKIL

GVNLILVSGFIITFLFKKHYHYLNLELLLLTLTFLSAYIILTISFVSKQIPKLVFYPFLIGFVVLRMTLNTFYQLNS

LNDEWIFPSRQGYAKYNHSISKLVRKTERNNSTFFRTERWLGQTGNDSMKYNYNGISQFSSIRNRSSSQVLDRLGFK

SDGTNLNLRYQNNTLIADSLFGVKYNLTEYPFDKFGFIKKAQDKQTILYKNQFASQLAILTNQVYQDKPFTVNTLDN

QTTLLNQLSGLKETYFEHLIPNSVSGQTTLNKQVFVKKNKQGNTEITYNITIPKNSQLYVSMPFINFNNEENKIVQI

SVNNGPFVPNTLDNAYSFFNIGSFAENSRIKVKFQFPHNDQVSFPIPHFYGLKLEAYQKAMTVINKRKVKVRTDHNK

VIANYTSPNRSSLFFTIPYDRGWKAYQNNKEIKIFKAQKGFMKINIPKGKGKVTLIFIPYGFKFGVGLSITGIVLFT

VYYFKFGKNKIG

SeqID 374

WFMKHSVGKRHNLVT

SeqID 375

MKDHLRCFLHYRSKNVGKNGTRLKGGKPND

SeqID 376

DKQLFQCVQGFQIRNLNGKKHGIKLIFIKNVKH

SeqID 377

NEEAIDGVSKLGKKITILGNNSYLSS

SeqID 378

IKQWLLVMKKMTVLCLKLLEILLLCKTAILN

SeqID 379

RRISNNLGVLKEATVIHAV

SeqID 380

ELCLKLSNVKPIYMPWHFEQISLKKQVSRVYHALFE

SeqID 381

RSCFSRCYPRFNRFRYHFLKPPWTRTNYCKRY

SeqID 382

RWFGFRYDDGRKFSFTNLL

SeqID 383

PLKSLSMTSRMKLLRLSQRKAM

SeqID 384

RHLRKRQKQNHLMNLCFLCAVKKLKKDNLI

SeqID 385

KLFIQPIQENMKQPIEIISNYYAKLGLMKI

SeqID386

YQTYSKKARKFGSVWLKPK

SeqID 387

TLLFQILLLLSHLKKNIFLEWNMHATYN

SeQID 388

KKFYEIILATKYHTSVFKWSLKLKNKLTNTKKISEASSGKLEIHLEQQLNSMPKVTLALILLVKKSNALL

SeqID 389

LKQMFRRVLHLLFQVVLIAVRSLMKMVLKSSWDGVTCSLSLLMRIIQYDYKVPLFQMMMLKGSLVLSKTKPRLTMMM

PLILEKYLKQITALVVAAEYLKVILFLKKPRDSF

SeqID 390

GRKINFSRPSRFE

SeqID 391

RVKYKKWEPLSISRSCYEKSSSTSSRI

SeqID 392

TWSRCCIPRCYSWCKIRRSNQCFGRWTSSFG

SeqID 393

KENFVQRLRSLLKAISKNIIIYSLVSLPLARHL

SeqID 394

SIINIKLEIYDICPKTISNRESIHGYRKLLPRLLFGDYLSKTLFQGFTSNHPNCRPIRL

SeqID 395

LSFRRSRRIGSSHSQI

SeqID 396

FIFKDRFKMRHFTD

SeqID 397

GLICACKAFISAILANLFCSSVIPIASCPENICAKPRTRFSSTSDIVLSPAKYIFKAPTVLSFIFRGTTIAEFN

SeqID 398

SSSLDTHYKHRELFLVADSLGSLWLILLVAYFV

SeqID 399

HLVPTQYHKQNLPLEYKRLNALVHK

SeqID 400

AHLHEGYHKYPLKRRNLYEFLRKL

SeqID 401

PLKELREHYIIPRKIRLQNFPHR

SeqID 402

KYTIAGSRVTQRRPSFSVTKV

SeqID403

AAFSSPAAKNRAFITTRSS

SeqID 404

SMVKQISFSDHLISRFEGLCLRRFSVRFRLRRSLSSVSNSTNNTSWL

SeqID 405

YHVLSRYHLNPLHVLQALYAHQEYVIVLIIHQRELFYRGQRGQ

SeqID 406

AQPSSTRISPKIDRFTPK

SeqID 407

WNRTINYFLNNLQLVTNIKLPTRKIFQSQNKFLSFIKN

SeqID 408

VQEGHKIFPLQR

SeqID 409

QQSNNTANYSRPYKPWHKKKNGLSCRCFS

SeqID 410

FFDDTKVNIHWLEMFAVCMCNIMWQCTNSSSLWENYFTLPIKATGSIDSRYKTTCCRFYITFNTGHLSSKEKIWVLT

CSKGLV

SeqID 411

VYSLRIATLELERNLIDKRRGKPTDSRSIEFLEVSLAILSCKNKLIPDIMLEKTFPLVSSEAKSEDLKFKSAISLNL

ENCS

SeqID 412

GHSNGIKEGNGGIFPVFCIAITATRFRFTINGFCNRINPCLCHFIVTLLSCCNRKS

SeqID 413

RIEKAIVPKPAISVLMIISRMVLGTPEKEPVICSANGIISIVIATPADIEIQMSKADIIKAKPVEKLFCENSLTELT

KAAPGTITISAPNIIMLRCSPNPEKYNKLALKATTELPRMVKRNPVCIRLSIKFRKYPKIKPVTAAENESNKELPKA

KEKLPAPQK

SeqID 414

PYSVTYAAPVICNGADLARRTAYAMSSSVTKPSDVEELVTIGDRTKRFSRLNPLGSINGRNKLPWTLFISAHRPLIS

HRLSLKSIKLCN

SeqID 415

ANHNDKNNTISVNKFSAK

SeqID 416

SFCVFCCHSKKGSYPHPKQSSWSSRFNSRSHTNDITCPYRCSKSSCKGSIT

SeqID 417

TLGISFKIRGCHFSAKPFLGGKSPDFPLPG

SeqID 418

FNISNSSLGFGTSAKSASTSSANSSLIISCDKATHSLQMKTPLPAIIFFTSSWLLPQNEQ

SeqID 419

GVYKKQSLSLIFHQIHLIVYHFDFLHDRFVPYYRE

SeqID 420

IFLRSVCGNSRQALGLTSYILHKLSSPKNGQGIALNTLSPSSSTSKNKSSKADNFIRKCLEILEISASVNRGPKVLQ

QLAHAVQSIS

SeqID 421

SRISTITIPIRISPTFLYREDFSSGLKGSFGLRLSSVKYFFPSKVYWRRPKNIPIAASEKPRWKFTSCPRYPTTMGE

IKAPRLIPKTKILKPASRRESFSPYKLPTISDTFGFNSPVPRIIRAIEMNRAV

SeqID 422

SFPRSNNLCAFKDKFKCRTSSRLDCL

SeqID 423

IHSFHRRKLNHHGKLNLYHLVLEKQPGLSSLLIMLYQSPDL

SeqID 424

LSFSIVSHLLTTIIIPLPASCANPAIRLSYSRIPSSALITIRTTSERSMARIERITEYFSVFSYTLPDLRIPAVSII

VYSCPWLSTKTVSIASRVVPATGLAITRSSPRMALIKLDFPTFGRPIKLKRIISGFSFSSSTGRFSTIASNISPVPI

P

SeqID 425

CPSSDIAGINFTKNSGKIDKQTVT

SeqID 426

PFLRNFHNNKTSTKFFSDSTKSRIGNTCHRC

SeqID 427

ASTGSAANPQTTPGKLLQASIAPIPVPQPVITKSAAPLLSKIAVSKPFCT

SeqID 428

AMPKRCFIPPEYDEIFFLPTSPKPTSSSNLGISCLAFFLSTPFKVAI

SeqID 429

LLLILKNPDTTHNTTIKRQPNKPPKVLRKRTEKKSIKLMKRIPINHVAP

SeqID 430

NFKARPPGVVSGLPNITPTFSLNWLIKIAIVFVLLMDADNLRIA

SeqID 431

KRVGICHIYHVSQSSCFHNNKFSRYFTQFSFNIIVHSIFLSQYFSFW

SeqID 432

NSTTSASLISGLISSRSGRRVNEPSSFSASNDTKAGRPKVASTASRIAGTFIDFSRTEITCPGVTRYDGISTRLPST

RI

SeqID 433

TRPLVEILVDKLAPYKEANFSRNSCTLGYLAFAVTNI

SeqID 434

RTRNAIRYPKASSGDYGTKREIITANKDKYSISKMCRWLNMPRSSYYYQAVESVSKTEFEETIKRIFLDSESRYGSR

KIKICLNNEGITLSRRRIRRIMKRLNLVSVYQKATFKPHSRGKNEAPIPNHLDRQFKQERPLQALVTDLTYVRVGNR

WAYVCLIIDLYNREIIGLSLGWHKTAELVKQAIQSIPYALTKVKMFHSDRGKEFDNQLIDEILEAFGITRSLSQAGC

PYDNAVAESTYRAFKIEFVYQETFQSLEELALKTEKATLFCTTFIKCCLFFRFLPML

SeqID 435

atggatgtat cgtctagtcc gaatattaca tttatgctgc aatacacaga ggctaatcct 60

caatatgtgg actatactaa cagagaagag gctgtcaaaa ttgatgaaga attgtcctta 120

gaaacgaaca ggcaaatgat tgaaggatta actgaagacg agttgactcg tattcaggaa 180

gctgtccctg aaacgcagtt gaattttagg gaatacattg attatatgaa ccgctcgtat 240

gcaactgaag aacaatctaa agaactaaca gctatcttta ctcaagaagc agattatctt 300

cagaaactac gattaatcga tctaaaaaat aagttggaat cagcttatca aaatggttca 360

cttctctggc aaggagttat ttcgtttgat aatgcttttc ttgcggaaca gggattgtat 420

gatgttgcga ctggtcaagt tgatcaaaaa gcgattaagg cagtgatgcg tgatatgatg 480

ccaacactta tccagaaaga gggcctttct gattctgctt tttggtgggg gaatatccat 540

ctgaatacag ataatatcca tatccatttt gggctttctg aagttgaatc taaccgtgag 600

aaaatattct atcagccacg tggacgtatg gagtacaaag gtaacttctc tcagaaaacc 660

atcaaccggt ttaaaagtgg tgtgtatcat ggattgctga aagaagaaac aagatccaat 720

cttctcagaa aagaacagat tttagctaat ctcaaagcgg acttcataac atctatttac 780

cagaaggaca agattacttc ttcagctgaa aaaaattttt tggaacaagc ctacaatcat 840

ttgccgctaa ataagaagtg gcgctatggt tctaatgcca gagattttgc ggttagtaag 900

ttctttcttg atcgctattt agattcctat ttaaacaatg aaggtagtgc tgcctatcaa 960

gaatttttga aagagactag agattttctt cagacttatg aaggggttta ttcagctgaa 1020

aaaaataaaa tctatgaaaa actacgtaaa gttgatgggc aaacgatcag aacgcttgca 1080

gaatcaaaag gatatgattt agaacatcat ttggcacgtc gtgtaatgga tttaagagag 1140

cgtttagcca ataatatctt acgttcgttt agagaagctg caccccaaat tcaagacgtt 1200

cagctggaaa aaaatttaga gagtttttct gttttgaacc agaagaaaat tttagaacaa 1260

catcctgaag caagtgtggt aaaaagtcag aaagcttggc agaagttagg ctactttgtg 1320

aaggctggag agcagccact tgaaattata aggccagtct ataaatctta tgataagcat 1380

ggtaaaggta taggacggcc agaatttgta tcagatactg tttatgatat tagtcagcta 1440

acagaaaata ttcagctgaa aagtctaacc ttgaaagacc tctctctgtt ttcttctaac 1500

gagttaaaag agttagtaga tgctgctaag ttaaagacta atccaacaga gagagaacgc 1560

cgtgaattag gtacctatcg ttatgcgttg aaacttagca tattagaatc tagtcagaag 1620

gaattgcagg ttcgtcaaaa gctactagaa caggtacagc cactagcgtc tgatcaacca 1680

tttttagatt ttaagaaaca gttaatagct caggaattac aagctatagc gctacaattg 1740

actcctaatt acaagctatc agaagatgat aaagccttga aaaatcgatt gaagaggcag 1800

tttgaagata gtgttgcgct acctgtttca aaagctactc ctggtgccat acaacttcct 1860

attaggcaac tttggactga gctaggattg gttcatcaca ttcaagatga aaacattcta 1920

acgcttctga aagggacatc aacgacgaaa caagcttata tagaagaact tcagactcat 1980

atctctattt ttcagttgaa atatcagatt aacaatagaa acaagcagat aagccagtta 2040

tcggatgaag caacaataaa ggagatgagg atagctaatg ctaaaggttt ctctgagcta 2100

aaacgtctat atgatacatt acagccatca gatgatggtc aaaatcagat tagtcaagct 2160

gtttctaaac aattacaaga acgaaaagtt atcaaaaaag ctcaattaca acagacgcag 2220

agaagcggaa aaatcaatac agacttcatg cgacaattga cagcttctct taatcgttca 2280

caacaagcaa gtaaaaaagc attgatggaa cgtgcacgta gtgatgaacg tgaggaacaa 2340

gaagaacgta ggcaagctca acgttaa 2367

SeqID 436

atgaattcta acacaaaagg tcacggattt ttccgcaagt caaaagcata cggcttagta 60

tgtgctattg cattagcagg tgcatttaca ttagctacta gtcaagtgtc tgctgatcaa 120

gttacaactc aagcaacaac tcaaacagta acgcaaaatc aagcagaaac agtaacatca 180

actcaacttg ataaagcagt agctacagct aaaaaagcag ctgtagctgt tacaaccaca 240

cctgcagtta atcatgcgac aactactgat gcacaagctg atttagctaa tcaaacacaa 300

gctgttaaag atgttactgc aaaagcacaa gctaatacac aagctattaa agatgctact 360

gctgaaaatg caaaaattga tgctgaaaac aaagcagagg cagagcgtgt tgcaaaagaa 420

aacaaggaag gtcaagcagc cgtagatgca cgtaacaaag caggtcaagc agccgtagat 480

gcacgtaata aagcgaaaca gcaagcgcaa gacgatcaaa aagcaaaaat tgatgctgaa 540

aacaaagcag agtctcaacg tgtaagtcag ttaaatgcac aaaataaagc aaaaattgac 600

gcagaaaata aagatgcgca agctaaagca aatgcgacta atgcacaatt acaaaaagat 660

tatcaagcta aattagcgga aatcaaatca gttgaagctt ataatgcagg tgtacgtcaa 720

cgtaataaag atgcacaagc taaagcagat gcgactaacg cacagttaca aaaagactat 780

caagctaaat tagcacttta taatcaagct ctaaaagcta aagcagaagc agataaacag 840

tctattaata atgttgcttt tgatatcaaa gctcaagcta aaggtgttga taacgctgaa 900

tatggaaact caatcatgac tgcaaaaact aaacctgacg gaagtttcga gtttaaccat 960

gatatgatcg atggtgtgaa gacaatcggc tatgggaaat tgacaggtaa agttaatcat 1020

cattatgttg ctaacaagga tggctctgtg acagcatttg ttgattctgt cactctttac 1080

aagtacgagt atcgtaatgt tgctcaaaat gctgctgtta accaaaatat tgtatttaga 1140

gttttaacaa aagatggtcg tcctattttt gaaaaagctc ataatggtaa caaaactttt 1200

gcagaaactt taaacaaaac tttacaactc aatcttaaat atgagcttaa accacatgct 1260

tccagcggta acgtcgaagt ctttaagatt catgatgact gggtacatga cacacatggg 1320

tctgctttag tgtcttatgt taataataat gatgctgttc ctaatgtggt catcccagaa 1380

cggccaactc caccaaagcc agtgaaagtt acacctgaag cagaaaaacc agtacctgaa 1440

aagccagttg agcctaaatt ggtaacgcct acattaaaaa cttatactcc agtcaaattt 1500

attccgcgag aatacaaacc agaaccaatc acccctgaga cgtttacccc tgagaaattt 1560

actccagctc aaccaaaagt gaaaccacat gtgtctattc ctgaaaagat taactactca 1620

gttagtgttc atcctgtttt agttccagct gctaatcctt caaaagctgt cattgatgaa 1680

gcaggtcaat ctgttaatgg taaaaccgta ttaccaaatg caacattaga ctatgttgct 1740

aaacaaaact ttagtcaata caaaggtatt aaagcttctg cagaagcgat cgcaaaaggt 1800

tttgcatttg tagatcaacc aaatgaagcg ttagctgaat tgactgttaa gtctatcaaa 1860

gcatctaatg gtgatgatgt atcaagcttg ttagaaatgc gtcatgtttt atcaaaagat 1920

actttagacc aaaaacttca atctcttatt aaagaggcag gaattagtcc agttggtgag 1980

ttttacatgt ggactgcaaa agatccacaa gctttttata aagcttatgt tcaaaaagga 2040

ctagatatca cttataatct atcctttaaa atcaaagcta actttactaa aggtcaaatc 2100

aaaaatggtg ttgcacagat tgattttggg aatggatata caggtaatat tgtagtcaat 2160

gatgttactg ttccagaagt acataaggat atacttgata aagaggatgg taaatcaatt 2220

aataatagta cggttaagtt aggtgatgaa gtgacctaca aacttgaagg atgggttgta 2280

ccagcaaacc gtggttacga tctttttgaa tacaaatttg tggatcaatt acaacacaca 2340

catgatcttt acttacgtga taaagtggtc gctaaagttg atgtgacatt aaaagatggt 2400

acagtcatta aaaaagggac taatttagga gagtacacag aaacagttta caacaaaacg 2460

acaggtcatt atgagcttgc ctttaaaaaa gagtttttag ctaaggtttc tcgtgaatca 2520

gaatttggtg cagatgattt tattgtagtt aaacgtatta aggcaggtga tgtttacaat 2580

actgctgatt tatatgttaa tggatataaa gttaagtcag aagcagttgt gactcatact 2640

actggaaaat caaaaccagt tgaaccacaa aaagcaactc caaaagctcc agctaaagga 2700

ctgccatcaa ctggtgaagc tagtatgacg ccacttactg caattggagc aattatctta 2760

tcagctctag gcctcgcagg ctttaaaaag cgtcaaaaat aa 2802

SeqID 437

atgtttagtt ggttggaagc tctttattac actctgatac aactggcaaa agtgaaccgg 60

ttgaatgctc ttttcttagt tagcgttgtg ggttatcttt gttaccaggg aataaaactc 120

gtcagaaaaa ccataagaaa cttttttcag ctgatgaagg gtttcatagg tgatagagag 180

aacatcaaga aatgcatcaa aaacaagaaa gaggcactag tccattcttg gaaacatcgt 240

caagatattg attggaaatc aactggaaaa gataagagta aacagttatg gaatcttatg 300

aagcgacttg cgacagttgc tccttcattt ttgttcttat tattgggaaa tgttctcttt 360

cgtctcattt atcaactgcc ttttgtaaag caagacagaa agcgatttga caaggaaatg 420

aagcccttgc tctacttcaa gaactatcgt agttttgtgt tcatgggaat aggtttcagt 480

ttcatagcgt ttattctcac aaactatttt gtgacggttt taagggctgc tattcgtttt 540

ctatatttct caatcatgac gttaagagat aatagccaag tcgttagctt taacgttgat 600

agtttgctca tccagaattt attcaatgct agggtatttg tgatagctcc cattctagca 660

gtgccaatct ttctcattgg tttagtcgta gcttggcggt ctgcttgggt taactttgaa 720

cagtaccgtg attataatca taatgaagaa ggggatgatc gctttgcgac tgtcaaagaa 780

atccaccagc aatataagaa agttcctaat aaaacggaaa cttatccagg tgaaggaggt 840

gtgcccgttc ttcatgaaac aagaaagaat ttgacaggct taacgcttaa atctcaaatg 900

ctttggcaaa accgtacctt tagtcgctat ttaacgaatg cggaaaggat tttagggctc 960

ttatcgacgc cttcaggaga ttattacatc gatgatagca ccacaaactt gatcaccatg 1020

gggattactc ggtcaggtaa gggagaagct catattgccc ctattattga tattaatagc 1080

cgtgcggaaa ttcaaccgtc actgattatt gcagacccta aaggggaaca ttaccagtcc 1140

tcttataaaa ccatgcgtcg gcgtggctat gatgttaatg tcctttcttt ccaaaacatg 1200

gattggtcca tgtcctataa ccctttagct cttgcgattg cagcagctaa gaagggttac 1260

tatgaaatga cacagacaag ggttaatgcg gttgcagaag ccatttatcg taaaacgaaa 1320

cctggtagcg gtaatggcaa tgcaaaatac tgggaagata cctccatttc cctctttaat 1380

gccattgcta tggccttaat ggaccgtgct aatgaaaccg tcaggaatgg tgaaaccgat 1440

gcttgggata ccgttaaagt tcgtaacatt gccaagtttt tgactgactt gggttctgaa 1500

gaagtctttg tcaatgattt tggagagatt gttgagaatc ctgataagaa ccaacaagtg 1560

aagaagaaat ctaaaatcac ggtttacttt gataacttgc gtaaaatcaa tcaagaacaa 1620

ttttccaaat ttagagatat ggctgattta aactttaggt cttctgactt cgcttcagaa 1680

gaaactaagg gaaatgtctt ctctagcatg atgtcaggta ttaacttatt cttgcaagat 1740

aatattgcta aactaacctc taaaaactct attgacctag aatcggttgg tttcccacgt 1800

cgcttgtcta tcaagtttcg ttctagttcc aatgtcgcta tgcgtaacga atacactcat 1860

aagacggcta aggttaccat tactagtcaa gctgtttggg gtaaaaccac taaacaagtt 1920

atccacgtag atgctgcaac agctctgatt gatggtgaag gctatctaac ctatgtgatt 1980

gaaccccagc ttcctgatca attcttggta acaattgact ttaatcacga aaacaatggt 2040

ggttcagcta ttcgtcacaa aactttccaa ttctcagctg agaaagtcta taagaaacgt 2100

ggtaacgtta ttacgttgga tgactacacg aaaaaaccag ttttggatca tatcaaagtt 2160

actgttctca acaagcaaga tgataacctt ctccagaaag aagatattga cctgatttat 2220

tcagataatc ctaaagtgat ttacttggta acacctccaa ataggactga atataatagt 2280

attgtatctc tgtttttgga tcaattgttt aatgccaatt atgagttagc tctgtcaaat 2340

ggtcgcaagt gtgtcaatcg aattcttcat atcctcgatg aattcacaaa cattccagct 2400

attcctcaca tggataccaa gatttccatt ggtcttggtc aaaatattct ctactatctc 2460

tggattcaga acttgaaaca gttagtcagt gaatatggcg agaatacagc ggaaaccatt 2520

cgggagaact gttctttgaa agtttatatc aaatctactg ccccagcgac caacgagtac 2580

ttcagtaaag agttggggac tcggaccatt acacgtcgca gaaggtcaag taatattcta 2640

gatgaagcta atccaaatgt ttccattgaa aatcctagac aggaactctt aacaccgaca 2700

cagctctcga aactccaaga aggggaagcg gttattttgc gtggtgttaa aggtcgagac 2760

aatgcaggtc ggaaaatcac aacggatccg attttcttgc atgagaaaac gagccttcct 2820

tatcgctaca tgttcttaca agaagaattt gaccagtcga tggctttggc agatattcca 2880

gtggaaagtg ggcataggga ccttgacctg caagatatag cagtaggggc acaaagcact 2940

tttaataaga ttattgattg gcggatggct ctaactgacc gtatgagaac aaatgggaag 3000

atacctcaat tagcatcaag aaaacaaacg attaaagctc taagtcaatc tcaatttact 3060

tctccagcag acctaacaca agctgtgatt gcagaggtat ttgatgagga agatgatgac 3120

gatcttttct ttgtggatga tgtcatgtaa 3150

SeqID 438

atgacagata atcgctttgc ccaattaaaa gaaaactttg aaaagggatc tcctaaaagg 60

cgagttccaa cgtctcgccc aatcgcagct caaaaagcgc ctgagagcta taacaaaaag 120

ggacggtatc cattttcgct ccaccaagat gtgcgttatg ataaattaga agcattagta 180

gcttatcatg gagctaagtc tgcatcagat tatctggaga ggttgattgt tcaggaatgg 240

gaaaagatgc agcggaagct taagaacaaa gaaaaataa 279

SeqID 439

atggcatatt tatcaaaatt atccgatttg gatccatcgt tgatggatgc ggattctgaa 60

caaatttata ttcctaaagt cttgtttgag cataatgact ttaaaggctt gacctacaaa 120

gagattttat tatattcttt tttgttaaat cgtttaagag agccattaga ttttattcaa 180

aaaggctatg atgataatga agatacctat gttcacttta aggtcgaaga tttatgtgaa 240

ctactcaacc agagtaagac aaccgttatt tccttgaaga aaaggttagc tcaatatggt 300

ttgatagaag aagtgaaagc aggtagtcac cagccgaatc gtatttattt aacagataaa 360

ttagttccat atattaaggg gtaa 384

SeqID 440

atgacaatct ttgatgaaag agagttaaaa gaacgattta ctcatgaaaa tagggttagt 60

ttttatgagt ttgtggctaa atatgatgct caaatggttc ctgtgatgaa agcaaagggc 120

tatcgatgta ttcattcaat ggagcgtaca gtggttttta cttttgggga attcacgatt 180

aggcgacgtc ggtggcaaaa aggagaacat tgggtggtgc cagttgatga aaagctagga 240

ctgaaaaaga atgttcgcta ctctttagaa ttcatgtatc aaattgctag tttagcaacc 300

atgatgcctt atgaaaaagt gattaaggtt gttcagatga tgtattgtat tgtgattacc 360

aaacctactg ttgtgaaggc cgttaagatt agtcgtgaat tgcttaaaga aaaggaagcg 420

tatcgttttt ttgatgaaga tataccagta gataaagaac cagttgatat gatttatctt 480

gaaggagatg gagtcatggt caaagctcga gaagaaggat tagataatcg caatgttgat 540

ttgtctcatt tcgtggttca tacgggtagt cagaaagtag gaagcaatcg ctttgtctta 600

caaaataaaa aagagtttgt gtcccttgat aatcgtcaaa cgcgtcaaaa gattttggac 660

tacctttaca atcattttta cattgcccca aacaccttgc ttattaccaa ttcagatggt 720

ggccatggct ataccccata tgtttttaaa gagattgcga aagcactcaa agtgaagcaa 780

cacgaacact tttgggatag gtatcatgtc aatgaaaaga tcaaaagttt ttttaaactc 840

tatccagtgg aactgatgac tggcgctttt cagagcatta aacagcatga taaagaaaag 900

cttagaactg ttttggatac gacagaagca ttgatactga tggaagaaga aatggaaggg 960

tttaaccagt tcaaacgaaa gttgttaaac aattttcaat atactaaatc agctgaattg 1020

agaggtttca gtcgtgcagg aataggtgtt atggagtcac aacatcgaaa aattacctat 1080

cggatgaaaa agcgcgggat gtattggaca attcagggag ctgagacgat gagtcaatta 1140

attgtcctat cgtatgaagg acagctaaga gatctcttct ttggttcttg gcgtgaagac 1200

tatcagaaat atcaagagct agaaaacctt agtgcgggaa aaatcaaaca tgaacagaat 1260

aaaatcaata agagatatga tctccagaca cttggtcggc ttaggtacgg taggcatcgt 1320

aatttatag 1329

SeqID 441

atggatgtat cgtctagtcc gaatattaca tttatgctgc aatacacaga ggctaatcct 60

caatatgtgg actatactaa cagagaagag gctgtcaaaa ttgatgaaga attgtcctta 120

gaaacgaaca ggcaaatgat tgaaggatta actgaagacg agttgactcg tattcaggaa 180

gctgtccctg aaacgcagtt gaattttagg gaatacattg attatatgaa ccgctcgtat 240

gcaactgaag aacaatctaa agaactaaca gctatcttta ctcaagaagc agattatctt 300

cagaaactac gattaatcga tctaaaaaat aagttggaat cagcttatca aaatggttca 360

cttctctggc aaggagttat ttcgtttgat aatgcttttc ttgcggaaca gggattgtat 420

gatgttgcga ctggtcaagt tgatcaaaaa gcgattaagg cagtgatgcg tgatatgatg 480

ccaacactta tccagaaaga gggcctttct gattctgctt tttggtgggg gaatatccat 540

ctgaatacag ataatatcca tatccatttt gggctttctg aagttgaatc taaccgtgag 600

aaaatattct atcagccacg tggacgtatg gagtacaaag gtaacttctc tcagaaaacc 660

atcaaccggt ttaaaagtgg tgtgtatcat ggattgctga aagaagaaac aagatccaat 720

cttctcagaa aagaacagat tttagctaat ctcaaagcgg acttcataac atctatttac 780

cagaaggaca agattacttc ttcagctgaa aaaaattttt tggaacaagc ctacaatcat 840

ttgccgctaa ataagaagtg gcgctatggt tctaatgcca gagattttgc ggttagtaag 900

ttctttcttg atcgctattt agattcctat ttaaacaatg aaggtagtgc tgcctatcaa 960

gaatttttga aagagactag agattttctt cagacttatg aaggggttta ttcagctgaa 1020

aaaaataaaa tctatgaaaa actacgtaaa gttgatgggc aaacgatcag aacgcttgca 1080

gaatcaaaag gatatgattt agaacatcat ttggcacgtc gtgtaatgga tttaagagag 1140

cgtttagcca ataatatctt acgttcgttt agagaagctg caccccaaat tcaagacgtt 1200

cagctggaaa aaaatttaga gagtttttct gttttgaacc agaagaaaat tttagaacaa 1260

catcctgaag caagtgtggt aaaaagtcag aaagcttggc agaagttagg ctactttgtg 1320

aaggctggag agcagccact tgaaattata aggccagtct ataaatctta tgataagcat 1380

ggtaaaggta taggacggcc agaatttgta tcagatactg tttatgatat tagtcagcta 1440

acagaaaata ttcagctgaa aagtctaacc ttgaaagacc tctctctgtt ttcttctaac 1500

gagttaaaag agttagtaga tgctgctaag ttaaagacta atccaacaga gagagaacgc 1560

cgtgaattag gtacctatcg ttatgcgttg aaacttagca tattagaatc tagtcagaag 1620

gaattgcagg ttcgtcaaaa gctactagaa caggtacagc cactagcgtc tgatcaacca 1680

tttttagatt ttaagaaaca gttaatagct caggaattac aagctatagc gctacaattg 1740

actcctaatt acaagctatc agaagatgat aaagccttga aaaatcgatt gaagaggcag 1800

tttgaagata gtgttgcgct acctgtttca aaagctactc ctggtgccat acaacttcct 1860

attaggcaac tttggactga gctaggattg gttcatcaca ttcaagatga aaacattcta 1920

acgcttctga aagggacatc aacgacgaaa caagcttata tagaagaact tcagactcat 1980

atctctattt ttcagttgaa atatcagatt aacaatagaa acaagcagat aagccagtta 2040

tcggatgaag caacaataaa ggagatgagg atagctaatg ctaaaggttt ctctgagcta 2100

aaacgtctat atgatacatt acagccatca gatgatggtc aaaatcagat tagtcaagct 2160

gtttctaaac aattacaaga acgaaaagtt atcaaaaaag ctcaattaca acagacgcag 2220

agaagcggaa aaatcaatac agacttcatg cgacaattga cagcttctct taatcgttca 2280

caacaagcaa gtaaaaaagc attgatggaa cgtgcacgta gtgatgaacg tgaggaacaa 2340

gaagaacgta ggcaagctca acgttaa 2367

SeqID 442

atgaattcta acacaaaagg tcacggattt ttccgcaagt caaaagcata cggcttagta 60

tgtgctattg cattagcagg tgcatttaca ttagctacta gtcaagtgtc tgctgatcaa 120

gttacaactc aagcaacaac tcaaacagta acgcaaaatc aagcagaaac agtaacatca 180

actcaacttg ataaagcagt agctacagct aaaaaagcag ctgtagctgt tacaaccaca 240

cctgcagtta atcatgcgac aactactgat gcacaagctg atttagctaa tcaaacacaa 300

gctgttaaag atgttactgc aaaagcacaa gctaatacac aagctattaa agatgctact 360

gctgaaaatg caaaaattga tgctgaaaac aaagcagagg cagagcgtgt tgcaaaagaa 420

aacaaggaag gtcaagcagc cgtagatgca cgtaacaaag caggtcaagc agccgtagat 480

gcacgtaata aagcgaaaca gcaagcgcaa gacgatcaaa aagcaaaaat tgatgctgaa 540

aacaaagcag agtctcaacg tgtaagtcag ttaaatgcac aaaataaagc aaaaattgac 600

gcagaaaata aagatgcgca agctaaagca aatgcgacta atgcacaatt acaaaaagat 660

tatcaagcta aattagcgga aatcaaatca gttgaagctt ataatgcagg tgtacgtcaa 720

cgtaataaag atgcacaagc taaagcagat gcgactaacg cacagttaca aaaagactat 780

caagctaaat tagcacttta taatcaagct ctaaaagcta aagcagaagc agataaacag 840

tctattaata atgttgcttt tgatatcaaa gctcaagcta aaggtgttga taacgctgaa 900

tatggaaact caatcatgac tgcaaaaact aaacctgacg gaagtttcga gtttaaccat 960

gatatgatcg atggtgtgaa gacaatcggc tatgggaaat tgacaggtaa agttaatcat 1020

cattatgttg ctaacaagga tggctctgtg acagcatttg ttgattctgt cactctttac 1080

aagtacgagt atcgtaatgt tgctcaaaat gctgctgtta accaaaatat tgtatttaga 1140

gttttaacaa aagatggtcg tcctattttt gaaaaagctc ataatggtaa caaaactttt 1200

gcagaaactt taaacaaaac tttacaactc aatcttaaat atgagcttaa accacatgct 1260

tccagcggta acgtcgaagt ctttaagatt catgatgact gggtacatga cacacatggg 1320

tctgctttag tgtcttatgt taataataat gatgctgttc ctaatgtggt catcccagaa 1380

cggccaactc caccaaagcc agtgaaagtt acacctgaag cagaaaaacc agtacctgaa 1440

aagccagttg agcctaaatt ggtaacgcct acattaaaaa cttatactcc agtcaaattt 1500

attccgcgag aatacaaacc agaaccaatc acccctgaga cgtttacccc tgagaaattt 1560

actccagctc aaccaaaagt gaaaccacat gtgtctattc ctgaaaagat taactactca 1620

gttagtgttc atcctgtttt agttccagct gctaatcctt caaaagctgt cattgatgaa 1680

gcaggtcaat ctgttaatgg taaaaccgta ttaccaaatg caacattaga ctatgttgct 1740

aaacaaaact ttagtcaata caaaggtatt aaagcttctg cagaagcgat cgcaaaaggt 1800

tttgcatttg tagatcaacc aaatgaagcg ttagctgaat tgactgttaa gtctatcaaa 1860

gcatctaatg gtgatgatgt atcaagcttg ttagaaatgc gtcatgtttt atcaaaagat 1920

actttagacc aaaaacttca atctcttatt aaagaggcag gaattagtcc agttggtgag 1980

ttttacatgt ggactgcaaa agatccacaa gctttttata aagcttatgt tcaaaaagga 2040

ctagatatca cttataatct atcctttaaa atcaaagcta actttactaa aggtcaaatc 2100

aaaaatggtg ttgcacagat tgattttggg aatggatata caggtaatat tgtagtcaat 2160

gatgttactg ttccagaagt acataaggat atacttgata aagaggatgg taaatcaatt 2220

aataatagta cggttaagtt aggtgatgaa gtgacctaca aacttgaagg atgggttgta 2280

ccagcaaacc gtggttacga tctttttgaa tacaaatttg tggatcaatt acaacacaca 2340

catgatcttt acttacgtga taaagtggtc gctaaagttg atgtgacatt aaaagatggt 2400

acagtcatta aaaaagggac taatttagga gagtacacag aaacagttta caacaaaacg 2460

acaggtcatt atgagcttgc ctttaaaaaa gagtttttag ctaaggtttc tcgtgaatca 2520

gaatttggtg cagatgattt tattgtagtt aaacgtatta aggcaggtga tgtttacaat 2580

actgctgatt tatatgttaa tggatataaa gttaagtcag aagcagttgt gactcatact 2640

actgagaaat caaaaccagt tgaaccacaa aaagcaactc caaaagctcc agctaaagga 2700

ctgccatcaa ctggtgaagc tagtatgacg ccacttactg caattggagc aattatctta 2760

tcagctctag gcctcgcagg ctttaaaaag cgtcaaaaat aa 2802

SeqID 443

atgtttagtt ggttggaagc tctttattac actctgatac aactggcaaa agtgaaccgg 60

ttgaatgctc ttttcttagt tagcgttgtg ggttatcttt gttaccaggg aataaaactc 120

gtcagaaaaa ccataagaaa cttttttcag ctgatgaagg gtttcatagg tgatagagag 180

aacatcaaga aatgcatcaa aaacaagaaa gaggcactag tccattcttg gaaacatcgt 240

caagatattg attggaaatc aactggaaaa gataagagta aacagttatg gaatcttatg 300

aagcgacttg cgacagttgc tccttcattt ttgttcttat tattgggaaa tgttctcttt 360

cgtctcattt atcaactgcc ttttgtaaag caagacagaa agcgatttga caaggaaatg 420

aagcccttgc tctacttcaa gaactatcgt agttttgtgt tcatgggaat aggtttcagt 480

ttcatagcgt ttattctcac aaactatttt gtgacggttt taagggctgc tattcgtttt 540

ctatatttct caatcatgac gttaagagat aatagccaag tcgttagctt taacgttgat 600

agtttgctca tccagaattt attcaatgct agggtatttg tgatagctcc cattctagca 660

gtgccaatct ttctcattgg tttagtcgta gcttggcggt ctgcttgggt taactttgaa 720

cagtaccgtg attataatca taatgaagaa ggggatgatc gctttgcgac tgtcaaagaa 780

atccaccagc aatataagaa agttcctaat aaaacggaaa cttatccagg tgaaggaggt 840

gtgcccgttc ttcatgaaac aagaaagaat ttgacaggct taacgcttaa atctcaaatg 900

ctttggcaaa accgtacctt tagtcgctat ttaacgaatg cggaaaggat tttagggctc 960

ttatcgacgc cttcaggaga ttattacatc gatgatagca ccacaaactt gatcaccatg 1020

gggattactc ggtcaggtaa gggagaagct catattgccc ctattattga tattaatagc 1080

cgtgcggaaa ttcaaccgtc actgattatt gcagacccta aaggggaaca ttaccagtcc 1140

tcttataaaa ccatgcgtcg gcgtggctat gatgttaatg tcctttcttt ccaaaacatg 1200

gattggtcca tgtcctataa ccctttagct cttgcgattg cagcagctaa gaagggttac 1260

tatgaaatga cacagacaag ggttaatgcg gttgcagaag ccatttatcg taaaacgaaa 1320

cctggtagcg gtaatggcaa tgcaaaatac tgggaagata cctccatttc cctctttaat 1380

gccattgcta tggccttaat ggaccgtgct aatgaaaccg tcaggaatgg tgaaaccgat 1440

gcttgggata ccgttacagt tcgtaacatt gccaagtttt tgactgactt gggttctgaa 1500

gaagtctttg tcaatgattt tggagagatt gttgagaatc ctgataagaa ccaacaagtg 1560

aagaagaaat ctaaaatcac ggtttacttt gataacttgc gtaaaatcaa tcaagaacaa 1620

ttttccaaat ttagagatat ggctgattta aactttaggt cttctgactt cgcttcagaa 1680

gaaactaagg gaaatgtctt ctctagcatg atgtcaggta ttaacttatt cttgcaagat 1740

aatattgcta aactaacctc taaaaactct attgacctag aatcggttgg tttcccacgt 1800

cgcttgtcta tcaagtttcg ttctagttcc aatgtcgcta tgcgtaacga atacactcat 1860

aagacggcta aggttaccat tactagtcaa gctgtttggg gtaaaaccac taaacaagtt 1920

atccacgtag atgctgcaac agctctgatt gatggtgaag gctatctaac ctatgtgatt 1980

gaaccccagc ttcctgatca attcttggta acaattgact ttaatcacga aaacaatggt 2040

ggttcagcta ttcgtcacaa aactttccaa ttctcagctg agaaagtcta taagaaacgt 2100

ggtaacgtta ttacgttgga tgactacacg aaaaaaccag ttttggatca tatcaaagtt 2160

actgttctca acaagcaaga tgataacctt ctccagaaag aagatattga cctgatttat 2220

tcagataatc ctaaagtgat ttacttggta acacctccaa ataggactga atataatagt 2280

attgtatctc tgtttttgga tcaattgttt aatgccaatt atgagttagc tctgtcaaat 2340

ggtcgcaagt gtgtcaatcg aattcttcat atcctcgatg aattcacaaa cattccagct 2400

attcctcaca tggataccaa gatttccatt ggtcttggtc aaaatattct ctactatctc 2460

tggattcaga acttgaaaca gttagtcagt gaatatggcg agaatacagc ggaaaccatt 2520

cgggagaact gttctttgaa agtttatatc aaatctactg ccccagcgac caacgagtac 2580

ttcagtaaag agttggggac tcggaccatt acacgtcgca gaaggtcaag taatattcta 2640

gatgaagcta atccaaatgt ttccattgaa aatcctagac aggaactctt aacaccgaca 2700

cagctctcga aactccaaga aggggaagcg gttattttgc gtggtgttaa aggtcgagac 2760

aatgcaggtc ggaaaatcac aacggatccg attttcttgc atgagaaaac gagccttcct 2820

tatcgctaca tgttcttaca agaagaattt gaccagtcga tggctttggc agatattcca 2880

gtggaaagtg ggcataggga ccttgacctg caagatatag cagtaggggc acaaagcact 2940

tttaataaga ttattgattg gcggatggct ctaactgacc gtatgagaac aaatgggaag 3000

atacctcaat tagcatcaag aaaacaaacg attaaagctc taagtcaatc tcaatttact 3060

tctccagcag acctaacaca agctgtgatt gcagaggtat ttgatgagga agatgatgac 3120

gatcttttct ttgtggatga tgtcatgtaa 3150

SeqID 444

atgacagata atcgctttgc ccaattaaaa gaaaactttg aaaagggatc tcctaaaagg 60

cgagttccaa cgtctcgccc aatcgcagct caaaaagcgc ctgagagcta taacaaaaag 120

ggacggtatc cattttcgct ccaccaagat gtgcgttatg ataaattaga agcattagta 180

gcttatcatg gagctaagtc tgcatcagat tatctggaga ggttgattgt tcaggaatgg 240

gaaaagatgc agcggaagct taagaacaaa gaaaaataa 279

SeqID 445

atggcatatt tatcaaaatt atccgatttg gatccatcgt tgatggatgc ggattctgaa 60

caaatttata ttcctaaagt cttgtttgag cataatgact ttaaaggctt gacctacaaa 120

gagattttat tatattcttt tttgttaaat cgtttaagag agccattaga ttttattcaa 180

aaaggctatg atgataatga agatacctat gttcacttta aggtcgaaga tttatgtgaa 240

ctactcaacc agagtaagac aaccgttatt tccttgaaga aaaggttagc tcaatatggt 300

ttgatagaag aagtgaaagc aggtagtcac cagccgaatc gtatttattt aacagataaa 360

ttagttccat atattaaggg gtaa 384

SeqID 446

atgacaatct ttgatgaaag agagttaaaa gaacgattta ctcatgaaaa tagggttagt 60

ttttatgagt ttgtggctaa atatgatgct caaatggttc ctgtgatgaa agcaaagggc 120

tatcgatgta ttcattcaat ggagcgtaca gtggttttta cttttgggga attcacgatt 180

aggcgacgtc ggtggcaaaa aggagaacat tgggtggtgc cagttgatga aaagctagga 240

ctgaaaaaga atgttcgcta ctctttagaa ttcatgtatc aaattgctag tttagcaacc 300

atgatgcctt atgaaaaagt gattaaggtt gttcagatga tgtattgtat tgtgattacc 360

aaacctactg ttgtgaaggc cgttaagatt agtcgtgaat tgcttaaaga aaaggaagcg 420

tatcgttttt ttgatgaaga tataccagta gataaagaac cagttgatat gatttatctt 480

gaaggagatg gagtcatggt caaagctcga gaagaaggat tagataatcg caatgttgat 540

ttgtctcatt tcgtggttca tacgggtagt cagaaagtag gaagcaatcg ctttgtctta 600

caaaataaaa aagagtttgt gtcccttgat aatcgtcaaa cgcgtcaaaa gattttggac 660

tacctttaca atcattttta cattgcccca aacaccttgc ttattaccaa ttcagatggt 720

ggccatggct ataccccata tgtttttaaa gagattgcga aagcactcaa agtgaagcaa 780

cacgaacact tttgggatag gtatcatgtc aatgaaaaga tcaaaagttt ttttaaactc 840

tatccagtgg aactgatgac tggcgctttt cagagcatta aacagcatga taaagaaaag 900

cttagaactg ttttggatac gacagaagca ttgatactga tggaagaaga aatggaaggg 960

tttaaccagt tcaaacgaaa gttgttaaac aattttcaat atactaaatc agctgaattg 1020

agaggtttca gtcgtgcagg aataggtgtt atggagtcac aacatcgaaa aattacctat 1080

cggatgaaaa agcgcgggat gtattggaca attcagggag ctgagacgat gagtcaatta 1140

attgtcctat cgtatgaagg acagctaaga gatctcttct ttggttcttg gcgtgaagac 1200

tatcagaaat atcaagagct agaaaacctt agtgcgggaa aaatcaaaca tgaacagaat 1260

aaaatcaata agagatatga tctccagaca cttggtcggc ttaggtacgg taggcatcgt 1320

aatttatag 1329

SeqID 447

atggctgtcg aaattattat gcctaaactc ggggttgata tgcaagaagg cgaaatcctt 60

gagtggaaaa aacaggtagg tgatgttgtt aatgaaggtg acgtcatact tgaaattatg 120

tcagataaaa caaatatgga gattgaagca gaagactcag gtgttttact aaaaatcaca 180

catgggaatg gagatgttgt tcctgtaact gagacaattg gctatatcgg tgctgaaggt 240

gaagaagtaa cagaagcatc ttcttctgaa aatacatcag ttgaagaaaa cgcgacacaa 300

gtaacttcag aaccagaaaa agttgaagaa acatcagaac caagtgtgcc agcagctaca 360

agtggtgaaa aagtacgtgc cactcctgca gctcgtaaat tagcaagaga gatgtctatt 420

gaccttgcac tagtatctgg aacaggggca aatggtcgcg ttcacagaga agatgttgaa 480

agcttcaagg gtgctcaacc acgtattaca ccattggcac gtcgaattgc tgaagatcaa 540

ggagttgata tagcagaaat cactggcagt ggtattcgtg gtaaaattgt taaaaatgat 600

gttttggctg ctatgtcacc gcaagcagca gaggctcctg tagaaacgaa agctacacca 660

acaacagaag aaaaacaatt accagaaggt gttgaagtca ttaagatgtc tgcaatgcgt 720

aaggctattt ctaaaggtat gactaactct tacctcactg ctccaagctt tacacttaac 780

tatgacattg atatgactga aatgatggct cttcgcaaga aattaattga tccaatcatg 840

gctaaaacag gcttgaaagt cagctttact gatttgattg gaatggccgt tgttaagaca 900

ttgatgaaac cagagcatcg atacctaaat gcttcattaa ttaacgatgc acaagaaatt 960

gagcttcata attttgttaa tattggaatc gcagttggtc ttgatgatgg cttgattgtt 1020

cctgttgttc acaatgctga tcaaatgtca ctttctgact ttgttatagc atctaaagat 1080

gttattaaga aaactcaaga aggtaaatta aaatcagctg aaatgtcagg ttcaacattc 1140

tcaattacta acttaggtat gtttggtact aagacgttta acccaattat caatcaaccg 1200

aactcagcaa tccttggagt aggcgctact attccgactc caacagtggt tgatggtgaa 1260

attgtagctc gaccaatcat ggcaatgtgt ttaacaattg accaccgtat tgttgatgga 1320

atgaatggtg ctaagtttat ggttgatctt aagaacttaa tggaaaaccc atttggttta 1380

cttatttga 1389

SeqID 448

atgtctagtt ttaatagaaa aaaacttaag tttttgggta tatctttggc gactctaaca 60

gccaccacag ttaccctggt tgcctgtggt aatgagtcaa agaactctgg agataataaa 120

gttattaatt ggtatattcc tactgaaatc agtacattgg acatttcaaa gaatacagat 180

gcttattcaa acttagctat aggtaattca ggcagcaatt tacttagaat tgataaagag 240

ggcaaaccaa aacccgattt agctaaaaaa gtatccgttt cttctgatgg tcttacttat 300

actgctacac ttcgtgataa tttaaaatgg tcagatggaa gcaaactaag cgctgaagat 360

tttgtttaca catggcggag aatagttgat cctaaaactg catctgaata tgcttacttg 420

gcgacagaat cgcacctttt aaatgctgat aagattaatt ctggagatat taaagattta 480

aataaacttg gtgtaacagc taaaggcaat caagtgacct ttaaattgac aagtccatgc 540

cctcaattta aatattattt agccttctct aattttatgc ctcaaaaaca atcgtatgtt 600

gaaaaagttg gtaaagatta tggtacaaca tcaaaaaatc aaatttactc aggtccttac 660

cttgttaagg attggaatgg ttcaaatggt aaatttaaat tagtcaaaaa taaatattat 720

tgggattcta aacatgttaa aacaaatagt gtcatagttc aaactatcaa aaaaccagat 780

actgcagttc aaatgtacaa gcaaggtcag attgattttg ctgaaatatc aggaacttct 840

gctatttata accaaactgg ttcggttaaa gcattgacaa atcagaaaat taggcaagct 900

ttgaatttag cgactgatcg taaaggtgtt gttaaagcgg cagttgatac aggttcaact 960

cctgctgaat ctctggtccc taaaaaatta gccaaattac ctaatggtga ggatctttcc 1020

aagtatactg ccccaggtta tacgtataat acaagtaaag ctcaaaaact ttttaaagaa 1080

ggtcttgctg aggttggtca gtcatctctc aagttaacaa tcacagctga ttcggactct 1140

ccagcagcta aaaatgctgt tgactatgtg aaatctacat gggagagtgc tttgcctggt 1200

ctaactgttg aagaaaaatt tgttactttc aagcaacgcc ttgaagatgc aaaaaatgaa 1260

aattttgatg tcgtgctctt ctcatgggga ggagattatc cagaaggatc aactttctat 1320

gggttattca cgactaattc tgcgtataat tatggtaaat tttcttctaa agagtatgac 1380

aatgcctatc aaaaggcaat tactactgac gccttgaaac caggagatgc cgctaatgat 1440

tacaaaactg ctgaaaaagc tttatttgat caatcatatt ataacccagt ttattaccta 1500

gggaaaaaag gactccaaaa ttctaaatta aaggggttgg taagaaattc aactggtttg 1560

aatgttgatt ttacttatgc ttataagaca gaataa 1596

SeqID 449

MDVSSSPNITFMLQYTEANPQYVDYTNREEAVKIDEELSLETNRQMIEGLTEDELTRIQEAVPETQLNFREYIDYMN

RSYATEEQSKELTAIFTQEADYLQKLRLIDLKNKLESAYQNGSLLWQGVISFDNAFLAEQGLYDVATGQVDQKAIKA

VMRDMMPTLIQKEGLSDSAFWWGNIHLNTDNIHIHFGLSEVESNREKIFYQPRGRMEYKGNFSQKTINRFKSGVYHG

LLKEETRSNLLRKEQILANLKADFITSIYQKDKITSSAEKNFLEQAYNKLPLNKKWRYGSNARDFAVSKFFLDRYLD

SYLNNEGSAAYQEFLKETRDFLQTYEGVYSAEKNKIYEKLRKVDGQTIRTLAESKGYDLEHHLARRVMDLRERLANN

ILRSFREAAPQIQDVQLEKNLESFSVLNQKKILEQHPEASVVKSQKAWQKLGYFVKAGEQPLEIIRPVYKSYDKHGK

GIGRPEFVSDTVYDISQLTENIQLKSLTLKDLSLFSSNELKELVDAAKLKTNPTERERRELGTYRYALKLSILESSQ

KELQVRQKLLEQVQPLASDQPFLDFKKQLIAQELQAIALQLTPNYKLSEDDKALKNRLKRQFEDSVALPVSKATPGA

IQLPIRQLWTELGLVHHIQDENILTLLKGTSTTKQAYIEELQTHISIFQLKYQINNRNKQISQLSDEATIKEMRIAN

AKGFSELKRLYDTLQPSDDGQNQISQAVSKQLQERKVIKKAQLQQTQRSGKINTDFMRQLTASLNRSQQASKKALME

RARSDEREEQEERRQAQR

SeqID 450

MNSNTKGHGFFRKSKAYGLVCAIALAGAFTLATSQVSADQVTTQATTQTVTQNQAETVTSTQLDKAVATAKKAAVAV

TTTPAVNHATTTDAQADLANQTQAVKDVTAKAQANTQAIKDATAENAKIDAENKAEAERVAKENKEGQAAVDARNKA

GQAAVDARNKAKQQAQDDQKAKIDAENKAESQRVSQLNAQNKAKIDAENKDAQAKANATNAQLQKDYQAKLAEIKSV

EAYNAGVRQRNKDAQAKADATNAQLQKDYQAKLALYNQALKAKAEADKQSINNVAFDIKAQAKGVDNAEYGNSIMTA

KTKPDGSFEFNHDMIDGVKTIGYGKLTGKVNHHYVANKDGSVTAFVDSVTLYKYEYRNVAQNAAVNQNIVFRVLTKD

GRPIFEKAHNGNKTFAETLNKTLQLNLKYELKPHASSGNVEVFKIHDDWVHDTHGSALVSYVNNNDAVPNVVIPERP

TPPKPVKVTPEAEKPVPEKPVEPKLVTPTLKTYTPVKFIPREYKPEPITPETFTPEKFTPAQPKVKPHASIPEKINY

SVSVHPVLVPAANPSKAVIDEAGQSVNGKTVLPNATLDYVAKQNFSQYKGIKASAEAIAKGFAFVDQPNEALAELTV

KSIKASNGDDVSSLLEMRHVLSKDTLDQKLQSLIKEAGISPVGEFYMWTAKDPQAFYKAYVQKGLDITYNLSFKIKA

NFTKGQIKNGVAQIDFGNGYTGNIVVNDVTVPEVHKDILDKEDGKSINNSTVKLGDEVTYKLEGWVVPANRGYDLFE

YKFVDQLQHTHDLYLRDKVVAKVDVTLKDGTVIKKGTNLGEYTETVYNKTTGHYELAFKKEFLAKVSRESEFGADDF

IVVKRIKAGDVYNTADLYVNGYKVKSEAVVTHTTEKSKPVEPQKATPKAFAKGLPSTGEASMTPLTAIGAIILSALG

LAGFKKRQK

SeqID 451

MFSWLEALYYTLIQLAKVNRLNALFLVSVVGYLCYQGIKLVRKTIRNFFQLMKGFIGDRENIKKCIKNKKEALVHSW

KHRQDIDWKSTGKDKSKQLWNLMKRLATVAPSFLFLLLGNVLFRKLIQLPFVKQDRKRFDKEMKPLLYFKNYRSFVF

MGIGFSFIAFILTNYFVTVLRAAIRFLYFSIMTLRDNSQVVSFNVDSLLIQNLFNARVFVIAPILAVPIFLIGLVVA

WRSAWVNFEQYRDYNHNEEGDDRFATVKEIHQQYKKVPNKTETYPGEGGVPVLHETRKNLTGLTLKSQMLWQNRTFS

RYLTNAERILGLLSTPSGDYYIDDSTTNLITMGITRSGKGEAHIAPIIDINSRAEIQPSLIIADPKGEHYQSSYKTM

RRRGYDVNVLSFQNMDWSMSYNPLALAIAAAKKGYYEMTQTRVNAVAEAIYRKTKPGSGNGNAKYWEDTSISLFNAI

AMALMDRANETVRNGETDAWDTVTVRNIAKFLTDLGSEEVFVNDFGEIVENPDKNQQVKKKSKITVYFDNLRKINQE

QFSKFRDMADLNFRSSDFASEETKGNVFSSMMSGINLFLQDNIAKLTSKNSIDLESVGFPRRLSIKFRSSSNVAMRN

EYTHKTAKVTITSQAVWGKTTKQVIHVDAATALIDGEGYLTYVIEPQLPDQFLVTIDFNHENNGGSAIRHKTFQFSA

EKVYKKRGNVITLDDYTKKPVLDHIKVTVLNKQDDNLLQKEDIDLIYSDNPKVIYLVTPPNRTEYNSIVSLFLDQLF

NANYELALSNGRKCVNRILHILDEFTNIPAIPHMDTKISIGLGQNILYYLWIQNLKQLVSEYGENTAETIRENCSLK

VYIKSTAPATNEYFSKELGTRTITRRRRSSNILDEANPNVSIENPRQELLTPTQLSKLQEGEAVILRGVKGRDNAGR

KITTDPIFLHEKTSLPYRYMFLQEEFDQSMALADIPVESGHRDLDLQDIAVGAQSTFNKIIDWRMALTDRMRTNGKI

PQLASRKQTIKALSQSQFTSPADLTQAVIAEVFDEEDDDDLFFVDDVM

SeqID 452

MTDNRFAQLKENFEKGSPKRRVPTSRPIAAQKAPESYNKKGRYPFSLHQDVRYDKLEALVAYHGAKSASDYLERLIV

QEWEKMQRKLKNKEK

SeqID 453

MAYLSKLSDLDPSLMDADSEQIYIPKVLFEHNDFKGLTYKEILLYSFLLNRLREPLDFIQKGYDDNEDTYVHFKVED

LCELLNQSKTTVISLKKRLAQYGLIEEVKAGSHQPNRIYLTDKLVPYIKG

SeqID 454

MTIFDERELKERFTHENRVSFYEFVAKYDAQMVPVMKAKGYRCIHSMERTVVFTFGEFTIRRRRWQKGEHWVVPVDE

KLGLKKNVRYSLEFMYQIASLATMMPYEKVIKVVQMMYCIVITKPTVVKAVKISRELLKEKEAYRFFDEDIPVDKEP

VDMIYLEGDGVMVKAREEGLDNRNVDLSHFVVHTGSQKVGSNRFVLQNKKEFVSLDNRQTRQKILDYLYNHFYIAPN

TLLITNSDGGHGYTPYVFKEIAKALKVKQHEHFWDRYHVNEKIKSFFKLYPVELMTGAFQSIKQHDKEKLRTVLDTT

EALILMEEEMEGFNQFKRKLLNNFQYTKSAELRGFSRAGIGVMESQHRKITYRMKKRGMYWTIQGAETMSQLIVLSY

EGQLRDLFFGSWREDYQKYQELENLSAGKIKHEQNKINNKYDLQTLGRLRYGRHRNL

SeqID 455

MDVSSSPNITFMLQYTEANPQYVDYTNREEAVKIDEELSLETNRQMIEGLTEDELTRIQEAVPETQLNFREYIDYMN

RSYATEEQSKELTAIFTQEADYLQKLRLIDLKNKLESAYQNGSLLWQGVISFDNAFLAEQGLYDVATGQVDQKAIKA

VMRDMMPTLIQKEGLSDSAFWWGNIHLNTDNIHIHFGLSEVESNREKIFYQPRGRMEYKGNFSQKTINRFKSGVYHG

LLKEETRSNLLRKEQILANLKADFITSIYQKDKITSSAEKNFLEQAYNHLPLNKKWRYGSNARDFAVSKFFLDRYLD

SYLNNEGSAAYQEFLKETRDFLQTYEGVYSAEKNKIYEKLRKVDGQTIRTLAESKGYDLEHHLARRVMDLRERLANN

ILRSFREAAPQIQDVQLEKNLESFSVLNQKKILEQHPEASVVKSQKAWQKLGYFVKAGEQPLEIIRPVYKSYDKHGK

GIGRPEFVSDTVYDISQLTENIQLKSLTLKDLSLFSSNELKELVDAAKLKTNPTERERRELGTYRYALKLSILESSQ

KELQVRQKLLEQVQPLASDQPFLDFKKQLIAQELQAIALQLTPNYKLSEDDKALKNRLKRQFEDSVALPVSKATPGA

IQLPIRQLWTELGLVHHIQDENILTLLKGTSTTKQAYIEELQTHISIFQLKYQINNRNKQISQLSDEATIKEMRIAN

AKGFSELKRLYDTLQPSDDGQNQISQAVSKQLQERKVIKKAQLQQTQRSGKINTDFMRQLTASLNRSQQASKKALME

RARSDEREEQEERRQAQR

SeqID 456

MNSNTKGHGFFRKSKAYGLVCAIALAGAFTLATSQVSADQVTTQATTQTVTQNQAETVTSTQLDKAVATAKKAAVAV

TTTPAVNHATTTDAQADLANQTQAVKDVTAKAQANTQAIKDATAENAKIDAENKAEAERVAKENKEGQAAVDARNKA

GQAAVDARNKAKQQAQDDQKAKIDAENKAESQRVSQLNAQNKAKIDAENKDAQAKANATNAQLQKDYQAKLAEIKSV

EAYNAGVRQRNKDAQAKADATNAQLQKDYQAKLALYNQALKAKAEADKQSINNVAFDIKAQAKGVDNAEYGNSIMTA

KTKPDGSFEFNHDMIDGVKTIGYGKLTGKVNHHYVANKDGSVTAFVDSVTLYKYEYRNVAQNAAVNQNIVFRVLTKD

GRPIFEKAHNGNKTFAETLNKTLQLNLKYELKPHASSGNVEVFKIHDDWVHDTHGSALVSYVNNNDAVPNVVIPERP

TPPKPVKVTPEAEKPVPEKPVEPKLVTPTLKTYTPVKFIPREYKPEPITPETFTPEKFTPAQPKVKPHVSIPEKINY

SVSVHPVLVPAANPSKAVIDEAGQSVNGKTVLPNATLDYVAKQNFSQYKGIKASAEAIAKGFAFVDQPNEALAELTV

KSIKASNGDDVSSLLEMRHVLSKDTLDQKLQSLIKEAGISPVGEFYMWTAKDPQAFYKAYVQKGLDITYNLSFKIKA

NFTKGQIKNGVAQIDFGNGYTGNIVVNDVTVPEVHKDILDKEDGKSINNSTVKLGDEVTYKLEGWVVPANRGYDLFE

YKFVDQLQHTHDLYLRDKVVAKVDVTLKDGTVIKKGTNLGEYTETVYNKTTGHYELAFKKEFLAKVSRESEFGADDF

IVVKRIKAGDVYNTADLYVNGYKVKSEAVVTHTTEKSKPVEPQKATPKAPAKGLPSTGEASMTPLTAIGAIILSALG

LAGFKKRQK

SeqID 457

MFSWLEALYYTLIQLAKVNRLNALFLVSVVGYLCYQGIKLVRKTIRNFFQLMKGFIGDRENIKKCIKNKKEALVHSW

KHRQDIDWKSTGKDKSKQLWNLMKRLATVAPSFLFLLLGNVLFRLIYQLPFVKQDRKRFDKEMKPLLYFKNYRSFVF

MGIGFSFIAFILTNYFVTVLRAAIRFLYFSIMTLRDSSQVVSFNVDSLLIQNLFNARVFVIAPILAVPIFLIGLVVA

WRSAWVNFEQYRDYNHNEEGDDRFATVKEIHQQYKKVPNKTETYPGEGGVPVLHETRKNLTGLTLKSQMLWQNRTFS

RYLTNAERILGLLSTPSGDYYIDDSTTNLITMGITRSGKGEAHIAPIIDINSRAEIQPSLIIADPKGEHYQSSYKTM

RRRGYDVNVLSFQNMDWSMSYNPLALAIAAAKKGYYEMTQTRVNAVAEAIYRKTKPGSGNGNAKYWEDTSISLFNAI

AMALMDRANETVRNGETDAWDTVTVRNIAKFLTDLGSEEVFVNDFGEIVENPDKNQQVKKKSKITVYFDNLRKINQE

QFSKFRDMADLNFRSSDFASEETKGNVFSSMMSGINLFLQDNIAKLTSKNSIDLESVGFPRRLSIKFRSSSNVAMRN

EYTHKTAKVTITSQAVWGKTTKQVIHVDAATALIDGEGYLTYVIEPQLPDQFLVTIDFNHENNGGSAIRHKTFQFSA

EKVYKKRGNVITLDDYTKKPVLDHIKVTVLNKQDDNLLQKEDIDLIYSDNPKVIYLVTPPNRTEYNSIVSLFLDQLF

NANYELALSNGRKCVNRILHILDEFTNIPAIPHMDTKISIGLGQNILYYLWIQNLKQLVSEYGENTAETIRENCSLK

VYIKSTAPATNEYFSKELGTRTITRRRRSSNILDEANPNVSIENPRQELLTPTQLSKLQEGEAVILRGVKGRDNAGR

KITTDPIFLHEKTSLPYRYMFLQEEFDQSMALADIPVESGHRDLDLQDIAVGAQSTFNKIIDWRMALTDRMRTNGKI

PQLASRKQTIKALSQSQFTSPADLTQAVIAEVFDEEDDDDLFFVDDVM

SeqID 458

MTDNRFAQLKENFEKGSPKRRVPTSRPIAAQKAPESYNKKGRYPFSLHQDVRYDKLEALVAYHGAKSASDYLERLIV

QEWEKMQRKLKNKEK

SeqID 459

MAYLSKLSDLDPSLMDADSEQIYIPKVLFEHNDFKGLTYKEILLYSFLLNRLREPLDFIQKGYDDNEDTYVHFKVED

LCELLNQSKTTVISLKKRLAQYGLIEEVKAGSHQPNRIYLTDKLVPYIKG

SeqID 460

MTIFDERELKERFTHENRVSFYEFVAKYDAQMVPVMKAKGYRCIHSMERTVVFTFGEFTIRRRRWQKGEHWVVPVDE

KLGLKKNVRYSLEFMYQIASLATMMPYEKVIKVVQMMYCIVITKPTVVKAVKISRELLKEKEAYRFFDEDIPVDKEP

VDMIYLEGDGVMVKAREEGLDNRNVDLSHFVVHTGSQKVGSNRFVLQNKKEFVSLDNRQTRQKILDYLYNHFYIAPN

TLLITNSDGGHGYTPYVFKEIAKALKVKQHEHFWDRYHVNEKIKSFFKLYPVELMTGAFQSIKQHDKEKLRTVLDTT

EALILMEEEMEGFNQFKRKLLNNFQYTKSAELRGFSRAGIGVMESQHRKITYRMKKRGMYWTIQGAETMSQLIVLSY

EGQLRDLFFGSWREDYQKYQELENLSAGKIKHEQNKINKRYDLQTLGRLRYGRHRNL

SeqID 461

MAVEIIMPKLGVDMQEGEILEWKKQVGDVVNEGDVLLEIMSDKTNMEIEAEDSGVLLKITHGNGDVVPVTETIGYIG

AEGEEVTEASSSENTSVEENATQVTSEPEKVEETSEPSVPAATSGEKVRATPAARKLAREMSIDLALVSGTGANGRV

HREDVESFKGAQPRITPLARRIAEDQGVDIAEITGSGIRGKIVKNDVLAAMSPQAAEAPVETKATPTTEEKQLPEGV

EVIKMSAMRKAISKGMTNSYLTAPSFTLNYDIDMTEMMALRKKLIDPIMAKTGLKVSFTDLIGMAVVKTLMKPEHRY

LNASLINDAQEIELHNFVNIGIAVGLDDGLIVPVVHNADQMSLSDFVIASKDVIKKTQEGKLKSAEMSGSTFSITNL

GMFGTKTFNPIINQPNSAILGVGATIPTPTVVDGEIVARPIMAMCLTIDHRIVDGMNGAKFMVDLKNLMENPFGLLI

SeqID 462

MSSFNRKKLKFLGISLATLTATTVTLVACGNESKNSGDNKVINWYIPTEISTLDISKNTDAYSNLAIGNSGSNLLRI

DKEGKPKPDLAKKVSVSSDGLTYTATLRDNLKWSDGSKLSAEDFVYTWRRIVDPKTASEYAYLATESHLLNADKINS

GDIKDLNKLGVTAKGNQVTFKLTSPCPQFKYYLAFSNFMPQKQSYVEKVGKDYGTTSKNQIYSGPYLVKDWNGSNGK

FKLVKNKYYWDSKHVKTNSVIVQTIKKPDTAVQMYKQGQIDFFAEISGTSAIYNQTGSVKALTNQKIRQALNLTDRK

GVVKAAVDTGSTPAESLVPKKLAKLPNGEDLSKYTAPGYTYNTSKAQKLFKEGLAEVGQSSLKLTITADSDSPAAKN

AVDYVKSTWESALPGLTVEEKFVTFKQRLEDAKNENFDVVLFSWGGDYPEGSTFYGLFTTNSAYNYGKFSSKEYDNA

YQKAITTDALKPGDAANDYKTAEKALFDQSYYNPVYYLGKKGLQNSKLKGLVRNSTGLNVDFTYAYKTE

SeqID 463

atgcttaaaa aatcgcactt tttacagata tttacacttt gcttagccct cttaacgatt 60

tctggttgtc aattaaccga tactaaaaaa cctggtcata ccacaattaa ggttgctgcc 120

caaagttcta cagagtctag tatcatggca aatattgtca ccgaattaat tcatcacgaa 180

ttaggataca acacaacttt aataagcaat cttggttcct ctacggttac tcaccaagct 240

ttgctccgtg gtgatgctga cattgctgcc acacgttata caggaacaga catcacagga 300

actcttggct taaaagctgt taaagacact aaagaagctt ctaagattgt aaaaactgaa 360

ttccaaaaac gctacaatca aacttggtat cctacttatg gtttttctga tacttatgca 420

ttcatggtta ctaaagagtt tgccagacag aataaaatca ccaagatctc tgatctcaaa 480

aagttatcaa caactatgaa ggcaggggtt gatagttcat ggatgaatcg cgagggagat 540

ggatacactg atttcgctaa aacatacggt tttgaatttt cacatattta ccctatgcaa 600

attggcttag tctatgatgc agttgaaagt aacaaaatgc aatctgtatt aggctactcc 660

actgacggtc gtatttcgag ctatgattta gaaattttaa gggatgataa aaaattcttt 720

cctccttatg aagcctctat ggttgtcaac aattctatca tcaaaaaaga tcctaaacta 780

aaaaaattac tccatcgact cgatggtaaa atcaatttaa aaacgatgca aaaccttaat 840

tatatggtag atgataaact tttagaacct tcagttgttg ccaaacaatt tttagaaaaa 900

aaccattatt ttagaggaga taaataa 927

SeqID 464

ttgaaaccgt ccttcaaaaa actattgcta ctatttagta ttatcaccat tttatccatc 60

gcctgtacac cacatgcaaa agcaagtggg cgttcatgga aaagttggtt tatcgaacaa 120

tacttttggc taaagcgcga taaaagttat tataaagttc aagatgaaag ctcatttcaa 180

aagtacttaa atgctagccg tgaacaatct gataagggtt attacttaga cccaaactct 240

gttaatggag ggcttgttca agagagactt tttgatatgc aagtctattc ttggaatgac 300

aacggtaaag ctaatcaaaa gaccattttc tatttagctg gtggctctta cttaaataat 360

ccaacgcctt accatatcag catgcttaag acgctctcaa ctagtcttga tgcaaaaatt 420

attttaccaa tttatccaaa aactcctcgt tatacctatg attatgctat ccctagattg 480

gttaaccttt atagacactt tcatgaaaaa aacgctaatc tcactttgat gggtgattca 540

gctggcggag gattagcact aggattagcg catgcattgt ctcaccaatc agggcaagag 600

gctattcctc aacctaaaaa tattatcctc ttatctccat ggctagatgt gacaatgaaa 660

catccagaaa ttcccaaata cgaagatact gatcctatcc tatctgcttg gggtctcgca 720

cgtgtaggtg aaatttgggc aaatggttct aataatacta accatacata tgttagtcct 780

aaaaatgccc ctgctactaa actcgctcct attacgttat ttactggaac acgagaaata 840

ttcttcccag atattcgcga ttatgctgca caactccaag cagctaatca ccccgttaac 900

tatattgcac aagagggaat gaatcatgtt tatccaattt accctattga agaagctaaa 960

acagcacagtatcaaatgat tgatatcatc aataaaactc cttaa 1005

SeqID 465

atgacaaaaa aacatcttaa aacgcttgcc ttggcactta ctacagtatc agtagtgaca 60

tacagccagg aggtatatgg attagaaaga gaggaatcgg tcaaacaaga acaaacccag 120

tcagcttcag aagatgattg gttcgaagaa gataatgaga ggaaaacaaa tgtttctaaa 180

gggaattcta ctgttgatga aacagttagt gatttatttt ctgatggaaa tagtaataac 240

tctagttcta aaaccgagtc agtggtaagt gaccctaaac aagtccccaa agcaaaacca 300

gaggttacac aggaagcaag caattctagt aatgatgcta gcaaagtaga agtaccaaaa 360

caggatacag cttcaaaaaa ggaaaccata gaaacatcaa cttgggaggc aaaagatttc 420

gtaactagag gggatacttt agtaggtttt tcaaaatctg gaattaataa gttatctcaa 480

acatcacact tagttttacc aagtcatgca gcagatggaa ctcaattgac acaagtagct 540

agctttgctt ttactccaga taaaaagacg gccattgcag aatatacaag taggctagga 600

gaaaatggga aaccgagtcg tttagatatt gatcagaagg aaattattga tgagggagaa 660

atatttaatg cttaccagtt gactaagctt actattccaa atggttataa gtctattggt 720

caagatgctt ttgtggacaa taagaatatt gctgaggtta accttcctga aagtctcgag 780

actatttcag actatgcttt tgctcacatg tctttaaaac aagtaaagtt accagataac 840

ctgaaggtca ttggagaatt agcttttttt gataaccaga ttggtggtaa gctttacttg 900

ccacgtcact tgataaaatt agcagaacgc gctttcaaat ctaatcgtat tcaaacagtt 960

gaatttttgg gaagtaagct taaggttata ggagaagcaa gttttcaaga taataatctg 1020

agtaatgtta tgcttccgga tggacttgaa aaaatagaat cagaagcttt tacaggaaat 1080

ccaggagatg aacattacaa caatcaggtc gtattgcgca caaggacagg ccaaaatcca 1140

catcaacttg cgactgagaa tacttacgtc aatccggaca aatcattgtg gcgtgcaacg 1200

cctgatatgg attataccaa atggttagag gaagatttta cctatcaaaa aaatagtgtt 1260

acaggttttt caaataaagg cttacaaaag gtaagacgta ataaaaactt agaaattcca 1320

aaacaacaca atggcattac tattactgaa attggtgata acgcttttcg caacgttgat 1380

tttcaaagta aaactttacg taaatatgat ttggaagaag taaagctccc ctcaactatt 1440

cggaaaatag gtgcttttgc ttttcaatct aataacttga aatcctttga agcaagtgaa 1500

gatttagaag agattaaaga gggagccttt atgaataatc gtattggaac tctagacttg 1560

aaagacaaac ttatcaaaat aggtgatgct tctttccata ttaatcatat tcacgccatt 1620

gttcttccag aatctgtaca agaaatagga cgttcagctt ttcgacaaaa tggtgcactt 1680

cacctgatgt ttatcggaaa taaggttaaa acaattggtg aaatggcttt tttatccaat 1740

aaactggaaa gtgtaaatct ctctgagcaa aaacaattaa agacaattga ggtccaagct 1800

ttttcggata atgcccttag tgaagtagtc ttaccgccaa atttacagac tattcgtgaa 1860

gaggctttca aaaggaatca tttgaaagaa gtgaagggtt catctacatt atctcagatt 1920

gcttttaatg cttttgataa aaatgatggg gacaaacgct ttggtaagaa agtggttgtt 1980

aggacacat aataattctca tatgttagca gatggtgagc gttttatcat tgatccagat 2040

aagctatctt ctacaatggt agaccttgaa aaggttttaa aaataatcga aggtttagat 2100

tactctacat tacgtcagac tactcaaact cagtttagag aaatgactac tgcaggtaaa 2160

gcgttgttat caaaatctaa cctccgacaa ggagaaaaac aaaaattcct tcaagaagca 2220

caatttttcc ttggtcgcgt tgatttggat aaagccacag ctaaagctga gaaggcttta 2280

gtgaccaaga aggcaacaaa gaatggtcat ttgcttgaga ggagtattaa caaagcggta 2340

ttagcttata ataatagcgc tattaaaaaa gctaatgtta agcgcttgga aaaagagtta 2400

gacttgctga cagatttagt cgagggaaaa ggaccattag cgcaagctac aatggtacaa 2460

ggagtttatt tattaaagac gcctttacca ttgccagaat attatatcgg attgaacgtt 2520

tattttgaca agtctggaaa attgatttat gcacttgata tgagtgatac tattggcgag 2580

ggacaaaaag atgcatatgg taatcctata ttaaatgttg acgaggataa tgaaggttat 2640

catgccttgg cagttgcaac tttagctgat tatgaaggtc tttatatcaa agatatttta 2700

aatagttccc ttgataagat taaagcaata cgtcagattc ctttggcaaa atatcataga 2760

ttaggatttt tccaagctat ccgaaatgca gcggcagaag cagaccgatt gattcctaag 2820

acacctaagg ggtacctaaa tacagtccca aattatcgta aaaaacaagt ggagaaaaat 2880

tcaaaaccag ttgattataa aacgccgatt tttgataagg ctttacctaa tgaaaaggta 2940

gatggtgata gagtggctaa aggtcataat ataaatgcgg agactaataa ttatgtagct 3000

gtaacaccaa taaggtccga gcagcaatta cataagtcac agtctgatgt aaatttacct 3060

caaacaagtt ctaaaaataa ttttatatac gagattctag gatacgttag tttatgtttg 3120

cttttcctag taactgctgg gaaaaaagga aaacgagcaa gaaaataa 3168

SeqID 466

atgaacaaaa aaattgccgg gatcggcttg gcttcgattg cagtacttag tttagctgca 60

tgtggacatc gtggtgcttc taaatctggt ggtaaatcag atagcttgaa ggttgcaatg 120

gtaacagata ccggtggtgt tgatgataaa tcatttaacc aatctggttg ggaaggtatg 180

caagcttggg gcaagaagaa tggccttaaa aaaggagctg gttttgacta tttccaatcg 240

gcaagtgaat ctgattatgc aactaactta gatacagctg tgtctagtgg ttataaattg 300

attttcggta ttggattttc tcttcatgat gctattgata aagcagcaga caataacaaa 360

gatgttaatt acgtcatcgt tgatgatgtt attaaaggga aagataatgt tgcaagtgtt 420

gtctttgcgg ataatgaatc agcttactta gcaggtattg cagccgctaa aactaccaaa 480

acaaaaacag ttggctttgt aggtggtatg gaatctgagg ttattacccg ttttgaaaaa 540

ggttttgaag caggtgtcaa atcagttgat aaatcaatta aaattaaagt tgactatgct 600

ggttcattcg gtgatgctgc taagggtaag acaattgcag ccgcacaata tgcttctggc 660

gcagatattg tttatcaagt agctggtgga actggtgcag gtgtattcag tgaagcaaaa 720

tcacgcaatg aatctctaaa agaagctgac aaagtttggg tattaggtgt agaccgtgac 780

caagcagcag aaggtaagta tacttctaaa gatggtaagg cttctaactt tgttttggct 840

tcatcaatta aagaagttgg aaaatcagtt gaattgattg ctactaaaac aagtaaaggt 900

aaattcccag gtggtaatgt aacaacttat ggtttgaagg atggtggagt tgatatagca 960

actacaaacc tttcagatga cgccgttaaa gcgattaaag aggctaaagc taaaattatt 1020

tcaggagata tcaaagttcc tagcaaataa 1050

geqID 467

atgaggattc aaacgacgaa ccttgataaa ggtggcggtg gaaaaagcag tcacacctat 60

aacgaaggag attggttgtc tagagttatg gggaaacgag tcttgttgat tgatggggcc 120

agagaatgta atttaacaca ctcctttgat gtgacgagcg acaaaacaat ctatgatatt 180

tttatcacag gagaatatga gatttgtcga atcaatgata agcttagctt aattagagga 240

gatgaacgac taactgatga acagctagat ttagccagtc gaaataataa ataccttcag 300

ttattcatgt ggttcagtga gcattatgag gaactggccg aacagtttga tattatcttg 360

attgatactc ataatgatga aagtcttgtt actgccaatt gtattgccgt ttctgatatt 420

gtcctagggg tgactgatgc gtcaactaat ggatttcgtg cctggttagc ccttaaaaag 480

tttgtcgatg acatcaagaa tgaagctatt gaggttatca caaagaaaag ctatgtgaag 540

gcagaagctt atctgattgg caataagatt gagtattatg gaaataatgt gacagagact 600

tgtaagcaat tcctggatgt tgtgcaggaa gatgaagctt atttgggttc aattcagaag 660

aaagaactga tggccaaaag tttagtcatt aatcagagtg tctttgaaca acgtgaacac 720

atgacagaaa aacagaagca agatcatcag aaattttatg ataatataga atatgtctat 780

caaaatattt taaccgtact agaaggagag actaaatga 819

SeqID 468

gtgaaaaaag ttgttttctt gaaaatactt gttccgcttg tctctttggt ctttcttatt 60

agcttgggta taacgatatt agcaggtgtt ttaggagcgg ttggtggttc gcagtcaaat 120

tgtacgatag aagttgtaac ctcaacctct acagactcgt ccatatcttc aggtgatggt 180

tccattgatt cttttgttaa ggagcacaaa gaggcttata tcctgtcttg gaaagctggt 240

ggcttcttac catctgcatc gattacgcag accatgattg aaaatggctt taacttcacc 300

aatcctaatg ggacctccct ctggcaagcc cataatatgg gaggggttaa aacgtctagt 360

aagagtaact tccccgttac cttagcaact tatggccaag atagtgttga cctttcagga 420

acaaagccag gagcaagagt cggcgatggc acaggtgggg cttatacctg gttttcaagc 480

tatgatgcag ggattgtcgg caaggcagag tttatggccc atcaaactct ctataccaag 540

gcaattaaca atatagatgg gatagctaca ctaagtgcga ttgcggatgg tggttgggcg 600

actgacggaa cttataaaac caagcttatt gacatgtatc ataagcttgg taaaacctat 660

gaatggctgg ataaggaagc tatcagcgct catggtgata aaccttataa agcaaccaca 720

gcttcagctg gagatgctgg actatctgat atttttacaa gcaatagtaa agattgtagt 780

gattcttctt caggtggtgc cactgatggt acaggaacag ttccagcaga tgcgatagcg 840

tggggatata ggcctgataa cctaccagag agtctgaagc agtatatcat tgatcctaaa 900

tcgcttggca tggcttatgc aaactcttct ggctggttta atccaggtag tgattatcta 960

gctggacaat gtgttaattt aacaatcagt cttggaaatc acctctgggg tcactcggga 1020

tctgtcagtg gcaatggaaa agaccaagct ggtgcttggg ccaatatctt tggcaattca 1080

gtcaaaacga ctcctaaaag aggagctatc ttttctaccc aaaacggagg tggcggttat 1140

ggccatactg ggattgtgtg ccatgtcttt gaaaatggat caatcttgat tgtggaacaa 1200

aatacgccat tatcgggagc tgattatttc aaaaaatctt acacatggaa ttaccgtatt 1260

tggacacctg cccaacaaaa agcagaagtc attagttttg cttacccata tggtaaagaa 1320

cctaaacttg gaaactaa 1338

SeqID 469

atgggaaaga cattttggaa agatatctat cgatcaatta cgacttcaaa aggtagattt 60

tcatcaatcc tcttacttat gatgttaggc tcatttgctt ttataggact gaaggtctca 120

gcacctaata tgcaaagaac agcacaaaat taccttgctc atcatcatgt tatggatatt 180

accgttttca attcttgggg acttgacaaa catgatcaaa ctgttttaga aagcttaaaa 240

ggatcacaag ttgaattttc ttactttgta gatacaaccc ctcaacagaa tagtaaatct 300

tatcgattgt attccaatac caaaacaata tcaacctttg atcttgttaa gggacgtctc 360

cctttaaata aatcagaaat tgctctctca tttcaggaac gaaaaaaata tgcaataggt 420

gataaaatta attttaaaca agataaaaac aaactgtttt caaatacagg tcctctaact 480

attgtaggtt ttgttaattc taccgagatt tggtctaaga caaaccttgg tagttctcaa 540

acaggggacg gcgacttaga tagttatggc gtattagata agacagcatt tcactctcca 600

gtatatacaa tggcaagggt aacttttaaa gatcttaggt tgattaaccc tttctcaata 660

agttacaaag aaaaagtagc gaaataccaa gaaaaggtat cgcgaaaatt aaatattcac 720

aataaaataa gatataccaa aacaaaaaaa gagagcctac gtaaaatcga tgaggaggaa 780

aaaagcctac taaaagctca aaaacaaatc aatcggttag ataatgatag cctagcgatg 840

ccactatctc aaagacaagc tattcaaatg aaaattaaac aagaccgact atctcttttg 900

aaacgaacaa aagagcttct aaaattgaga cataacaccc aaataatgga atcgcctcaa 960

attattgtct acaaccgtac tacctttcct ggaggacaag gatataacac ttttgactcc 1020

agtacaaata gcacttctaa aatcagtaat cttttcccca ttattttata tttagtcgca 1080

gcattagtaa ccttaacaac aatgactagg ttcgtcgagg aagaaagaac caacgcaggc 1140

atattaaaag cactaggcta tagtgaccgc caagttatct ttaaatttat tatttatggt 1200

tttatagcag gaacattagg gactactttg ggtatcattg gagggcatta tcttttacct 1260

cgtattattt ctgatatcat ttccaaagac ctgactattc caaacacaca gtatcatctt 1320

ttcttaaatt acagtttact agcctttgtt ttttcattat taagcattgt tcttccagtt 1380

tttgttatca ctcgacgtga gttgaaagaa aaagcagcat ttttattatt accaaaacca 1440

ccagctaaag gatctaaaat agctttagag tatatcaatt ggatttggaa aaaactgtcc 1500

ttcacacaaa aagtaactgc tagaaatatt tttcgttaca aacaaaggat gattatgacc 1560

atatttggag ttgctggatc agtagcgctc ttattttcag gtctaggaat acaatcctcc 1620

ttaaagcaaa ccgtcaatga acatttcggt cgtattatgc cctatgatat attgctaaca 1680

tataacacaa atgcttctcc cccaaaaata cttgaactac tttcaaaaga ttcaaaaata 1740

gacaaatacc aaccaataca ccttgaaaat cttgacgaat ccattcctgg acaaattaat 1800

aaacagtcaa tttccctatt cataacagac aaaaaacaat tactaccttt tatttattta 1860

caagaagcaa caactaataa gtcattgcac ttgaataata aagggattat tatttctaaa 1920

aaattagctc aattttatca tgttaatacc ggtgatttta tccatttatc tcattcacaa 1980

acacttcctt ctagaaaatt aaaaataaca ggagttgtca atgcgaatgt tggtcactat 2040

atttttatga caaaacaata ctatcgaact atttttaaga aagaaactaa agataacgct 2100

tttttggtta agttaaccaa acataaaatc gcaaataact tagcagaaaa acttttagaa 2160

attaatggag ttgagtctct tacgcaaaat gctcttcagc tagctagtgt agaagccgtt 2220

gtgcgttctt tagatggctc tatgactatt ttagttgtcg tatctttatt gttagccatt 2280

gtaattctct ataatctcac taatattaat ttagcagaac gtaagcggga gctatcaact 2340

ataaaggtat taggttttta taatgaagaa gtaactttat acatttaccg cgaaaccatt 2400

attttatcaa ccatcggtgt gattctaggc accattagcg gtacttattt acaccgtcaa 2460

atgatgttgc taattggttc agatcaaata ctttttggtg aaaaagtatc accaactaca 2520

tttataatac caataagcgt agtagtcatc attctaataa gtctatgttt tatagttaac 2580

catcaattaa aaaaactcaa tatgttagat gccttgaaat cagtagatta g 2631

SeqID 470

atgaataaaa gaaaattatt aacagtctta tcaattgcatcagttgcagt tttatcaagt 60

gttacttttg cagatgaaat aactccatct gatccaacag caccatcaac accccttatt 120

actccaaccc cagtagatcc tggaacacca actgatacga caaaacctag tactccagta 180

gatcctggaa cgccaactga tacgacaaaa cctagtactc cagtagatcc tggaacacca 240

actgatacga caaaacctag tactccagta gattcaggga cttcgactaa tccctcagat 300

tcagaagcaa caaatacaaa tctaggtaca aaaccaacag atgtagttaa gccagagaca 360

cctaaaatcc ctgaagtgcc gtcaacagta actccagatg tgattgacaa ggtagacaaa 420

gaaacaggta atattacgat taaaccaatt caaatcagcc ctgataaaac gattattgga 480

acacaaaatg gtaatgtcat tattcgagat agttcaggca gccacttagt atctgcaagt 540

gaagttggtg gtcgacttaa tgaagatggg acagtaacaa ttaaagattc gtcagggaaa 600

acaaaaacac tccctcgtac tggagaagct aaaggattct taagcattat tggtgccaca 660

atcctatctt ttgtagctta tttattcaag cagagagtaa ctttaaatta a 711

SeqID 471

atgagaccta tcgttagaaa aaagatgtac aaaaaaggta aatttttggt cattgcaggt 60

atcgttacta ttttaggtgg tagtgcaatt cttggtcaag atgtcaaggc agaacaggca 120

gaagcagcaa cttcaacgat ctcagagaaa actgatagca gtcagactat ttcggatact 180

tccaagttaa cgttacctgt taattcctcg gaggctatga aaaatagtgc agagccttta 240

ataaaaacag gctttgctac atctgtttca tctaatccta gagagatagc agctacccca 300

gtcaaaacct ttgatgctag ttcaaaagtt gttgtaaaag ctagtactgc tgagcactca 360

gcaaatcaaa caaattctaa tgttaatcaa gtagctaatg atagtgaagt tattactcaa 420

caaaattcaa caaagcaatt accaaaagtg acttactctg ctcatgttca agatattgga 480

tggcaaaagt cggttgataa tgcaacagtt tcaggaacag taggacagga aaagcaggtt 540

gaggcaataa aattgtctat taaagcccct gaaggaataa caggaaaact tagctataaa 600

acttatgtta aaggacaagg ttggcaacct tcagtagaga gtggtcaagt tagtggtact 660

gttggtcaga gtagacctat tgaagcacta agcattaacc ttactgataa tttacaaaaa 720

ttatatgatg tctattatcg tgttcatgtt caagatatcg ggtggatggc ttgggctaaa 780

aatggagctt acgctgggac tttgggaatg tcaaagcgcc ttgaagctta cgaagtaaag 840

tttaccctta aaggacaatc tgttttaaca ccaactatac ttaaggaaga gagacctgtc 900

ttaaattatc aggttaaggt aggccaaaat ggttggcaat ctaataaact tgaaggacaa 960

atggcgggaa ctttaggaga atccaaagcc ctagatggag taaaattcac tcttagcacc 1020

ttgaagtatg gggacattct ctatcgtact catgtacaag ataaaggttg gggagaaatt 1080

ttaggtagtc aaacaagtaa ggattatcaa ggaaaaagac tagaagctat tcaattggac 1140

ttaagtggga atttaaaaca aaattatgat atttattatc gcgctcacgt ccaagataaa 1200

ggttggatgg cttggcaaaa aaataatagt gtagcaggta cagtcggaga atcgaaacgt 1260

ttagaggctt tggaagtaaa actagtagct aaagcttcag attttagttc ccaaacaaat 1320

cattcattct tagaaatgaa gcatcctgga ttaagttatc aaacttatct tcaaaaagat 1380

ggttggaaac caacggtttt ggaaggccaa ttaggaggta gtattggact ttcaaaatct 1440

ataaaagcca tcaaacttaa cttaggtagt acagctctag gaaatataga atatagaact 1500

tttcttaatg gttcaggttg gcaaactgtt gttaattcag ggagagaaag taatgtacca 1560

aatgaatctc aacaagtaga agctattcag atgcgattga caggtctgct atcgaaaacc 1620

tatagtgtga tgtatcgagt tcacatgcaa gattatggtt ggcaggattg gacttataac 1680

aatgacatcg ctggtagtac taaccaagga aaacgaattg aagcaatcga aattaaatta 1740

gttgagactg ctaaaatacc tataactgtc caaacgacct ataagggaac tggaaactat 1800

aatgtacgag tgacaaatgt tattagtcct gggaatttaa aaattgctat ttggtcagat 1860

aaaaacaatc aggatgacct taagtggtat accgtcacac ccaaaaatca tgaagctaga 1920

ttgacattta atgttacaaa tcatagagac aatggtaaat actttatcca tgtttatcag 1980

gaaaatgctt ctaatcaaaa acaattattg gtaaaaactt ctctacaagt cgcacattca 2040

aattataata ctccttactt tagccaacgt gatggaaggt gggcaagtcg aagatatggt 2100

atggcaactt taggagaaac tggttgtgta ccaacttctc tagctatgat tttgtcttct 2160

ttgaaaggag aaactgtttt accaacgcaa attgcagatt acctttacca taagacagtt 2220

gaatttaatc gtggtgttca aggaacaact tctagaggaa tcttaaaagc agccaaagaa 2280

tggggtgtaa cagcaactgc attaggaact caagctaacc ttgtacaagc gttgaaggat 2340

ggttatcatg tcctagcggc agtacaaaat aatgtttttg ttcttcatgg aagtcatgaa 2400

atcgttctca aaggatataa taacggactg acacatgtct ctgaccctta cacaccgtcc 2460

ttgtctggtt ggtatccaat ttcacaactt tggaaagaac agagctatta tagtgaggat 2520

agaattgata ttggagaacc ttttgtaaag gtaacagatg cctaa 2565

SeqID 472

atggcaagaa tcccttatca gaaaacactt attttttgtg tagccgttat tattgctatt 60

tttattttgg gattatctaa ggatttagca caatctaaag ggcaaaaggt tgcaaataat 120

aatactgtaa aaacagcacg tgtggttgca aacggagata ttttgctgca cgatgtacta 180

tatgacagtg cgcgccaacc cgacggcact tataatttta ctccttattt taaagaggta 240

aaatcttgga ttgagagcgc tgatttagcg attggtgatt atgaagggac gattagttca 300

gaatatcctt tagcaggcta tcctttattt aatgctccta atgagattgc aactactatg 360

aaagaaactg gatatgatgt cgttgactta gcacataatc atattctgga ttcacaactg 420

gcaggtgcta tcaatactgt caagactttt aatcgtatgg gattggatac tattggggtt 480

tatttaaagg atagaaataa agaagatatc ttaattaagc gtgtaaatgg tataaaaatt 540

gctattttag gttattcgta tggctacaat ggtatggaag ctaatgtttc aaagagtgac 600

tatgaaaaac atatgtctga tttagatact aaaaagatta agcaagatat taaaaaggct 660

gaaaaagaag cagatatcac cattgtcatg ccacagatgg ggatagaata tcaaaaaaaa 720

cctactacag aacaagtaat gctttatcac agtatgatta aatggggggc agatattata 780

ttcggaggac accctcacgt tgttgaaccg tctgaagtga taaaaaaaga tggccagaaa 840

aaatttatta tttactctat gggaaatttt atttcaaatc agcgtttaga aacagttgat 900

gatatctgga cagaaagagg attattaatg gatgtaacaa tagagaaaaa aggtcaaaaa 960

acagtaatta aaaaagttaa ggcgcatcca acactagtag aagcaaaagc aaatggccgt 1020

tatgttgaag aaggctttcc agcttatgat tataggacgc tagttttaga ggattatatt 1080

gaaggtggta agaaacgttc tacgattgat cagactatga aagataaagt tgataaagca 1140

tataaggaga tgaatgagtt agttaatttg aagtggtaa 1179

SegID 473

atgaaacatt tctttgatta caagacaatg tctgtcgcac gtaaactcag cattagtttt 60

atcgctgtta tcctattggg aagtatttta ctttcacttc caatttttca atatgctaac 120

gcccctaaaa cacattacat tgatcacctt ttcacgactg tttcaatggt atgtgtaact 180

ggtttatctg tttttcctat cagtaaggtt tataacggtt gggggcagat tgtagctatt 240

ttactcatgc aaacaggggg acttggttta gtaacactaa tgtcgttgtc ttactatacc 300

ctaagacgta aaatgtcgtt aaatgatcag acccttttgc agtcagcgat aacttataac 360

agttcgacag atttaaaaaa atatctctat atgattttta aagtaacttt aactttagaa 420

gttttagcag ccagcatttt agccatagat tttattcctc gatttggttt gggtcatggt 480

atctttaata gtatattctt agctgtctca gccttttgta atgccggttt tgataatcta 540

ggggcaacga gcttagctca atttaaacta aatcctttgg ttaacattat cgtttgtttc 600

ttgattattt caggaggact tggatttgcc gtatggaaag atttaattga agctactata 660

caaacatctc acaagggacc aaaattaatc aagacatttc caaaacgcct cagcaaccat 720

tctaaactgg ttttaaaaac aacaaccatt attttactaa caggaacttt gctttcttgg 780

ttgttagagt ttggaaattt tagaacaatt gctaatttat ctctacctaa acaactaatg 840

gtcagctttt tccaaacagt tacgatgcgt accgctggat tttcaactat tgattatacc 900

caaactgatt tcgcaaccaa ccttgtttat attatccaaa tgttaattgg aggagctcct 960

ggtggaacgg caggaggatt taaagtcact gttatcgcca ttttactctt actatttaag 1020

gccgaactga gtggccaaag ccaagtgact tttcattatc gaacaattcc ttccagtatt 1080

attaaacaaa cactatcgat tttaactttc ttttttatca tacttattag cggctatctc 1140

cttcttctgg aactcaatcc ccatatagat cctttttctc ttttctttga ggcatctagt 1200

gccttagcaa ccgtgggtgt gacaatgaac actaccaatc aattaagtct gggcggtcga 1260

attgtaatta tgttcctcat gtttattggt cgagtcggtc caattaccgt actgctatca 1320

attcttcaga aaaaagagaa ggaaattcac tatgctgaaa cagaaattat tctaggttag 1380

SeqID 474

atgttagacg aattaaaagc aatcataaaa agcccaaaat tatggattac gatggctggg 60

gttgcactga ttccaacgct atataatgtt atctttctaa gctcaatgtg ggatccttat 120

gggaatacta aaaatttacc tgtggcagtc gttaaccaag ataaatcggc taaattaaat 180

ggaaaaacta tttcaattgg caaagatatg gaggacaatt tgtctaaaaa tgatagctta 240

gatttccatt ttacaacagc taaaagagca gaaaaagaat tagaaaaggg tcactattat 300

atggttatta cctttcctaa agatctttct agaaaagcga caactctcat gacagaaaaa 360

ccagaaaggt taaatattac ttataaaact actaaggggc gtagttttgt agcttcaaaa 420

atgagtgaga cagcagctaa taaactaaaa gatgaggtag cagaatctat tacagggact 480

tatactgaat cagtttttaa aaatatgggt tctatgaaaa caggtatcaa taaagctgct 540

gatgggagtc aagaattatt aaatggttct aataagctac aggatgggag ccaaaccttg 600

acaagtaatt tagatgtttt agcaagctct agtcaaactt tttctggagg agctaataaa 660

ctaaatagtg gaataaacct ttatacagat ggtgtgggga ccctatctaa cgggttagaa 720

acattatcag atggtgttac tgcttacaca acaggtgtgc ataagttgag tgaaggtagt 780

caaaaattag atgataagtc tcaagcattg gtggaaggat cagaaaagtt gacggatggt 840

ttacaacaat tgtcacaagc aactcaacta aaaccagagc aagaacgtac tttgcagaac 900

ctttcagatg gattaaagaa tctaaatcag attattacaa atttacaatc gacagcaact 960

actgatagcg atactaatag taagttattt aattttttat caactattga atcaagtaca 1020

aaggctttga tgaatacggc tgctgctgat aaacaaaagc aaatgactgc ggtacaatca 1080

acgtcagctt ttaaaagttt aacccctgaa caacaatctc aaatcacctc agcggttact 1140

ggaacaccta cttcagctga gacaattgca gcaaatattt cttcaaatat agaaaatatg 1200

aaaactgtgc ttagtgaagc atctagttca gccccttcta ataatggatc acaaaatctt 1260

caaactttgt caggaacagc taacaattta gtattaaaag ctatctcaga tttagataaa 1320

attcaaaaat tgccaacggc tactaagcag ttatatcaag gtagtcaaac cttgacaaaa 1380

ggaatcacgg attatacgaa tgctgtaggg caattacgca aaggtgcagt aactttagat 1440

agtaaatcca atcaattgat ttcaggaaca caaaaagcaa gtcagggagc tcaaacttta 1500

gatagtaaat cggatcaatt gagagacgga gcggggcaat tagctagtgg ttctgataga 1560

attgcagatg gttctaataa gcttgctggg ggaggacatc agttgataga tgggttgaca 1620

gagttgtcag gtggtgtttc tcaattatca agttctttgg ggaaagctgg ggaccagttg 1680

tccatggttt ctgtaaataa agataatgcc aatgcagtat ctagcccagt cactatcaag 1740

catgaagatt atgatagcgt tgatacgaat ggtgttggaa tggcaccata catgatttca 1800

gttgcgttga tggtggtagc tttatcagcc aatgttattt ttgcgaaagc tttatctggc 1860

aaagagccag ctaatcgttt tagttgggct aaaaataaat tattaatcaa tggattcatt 1920

gcaactctag cagcaactat cttatttttt gcagttcaat tcataggtct taaaccagat 1980

taccctggaa aaacctactt tattatccta ttgacagcat ggactttgat ggcattagta 2040

actgctttag tgggatggga taataggtat ggttccttct tgtcgttatt aatattatta 2100

ttccagcttg gttcaagcgc aggaacttac ccaatagaat tgagtcctaa gttctttcaa 2160

acaattcaac catttttacc gatgacttac tctgtttcag gattaagaga gaccatctcg 2220

ttgacgggag acgttaacca tcaatggaga atgctagtaa tctttttagt atcatcgatg 2280

atacttgctc ttcttattta tcgtaaacaa gaagattaa 2319

SeqID 475

MLKKSHFLQIFTLCLALLTISGCQLTDTKKPGHTTIKVAAQSSTESSIMANIVTELIHHELGYNTTLISNLGSSTVT

HQALLRGDADIAATRYTGTDITGTLGLKAVKDTKEASKIVKTEFQKRYNQTWYPTYGFSDTYAFMVTKEFARQNKIT

KISDLKKLSTTMKAGVDSSWMNREGDGYTDEAKTYGFEFSHIYPMQIGLVYDAVESNKMQSVLGYSTDGRISSYDLE

ILRDDKKFFPPYEASMVVNNSIIKKDPKLKKLLHRLDGKINLKTMQNLNYMVDDKLLEPSVVAKQFLEKNHYFRGDK

SeqID 476

LKPSFKKLLLLFSIITILSIACTPHAKASGRSWKSWFIEQYFWLKRDKSYYKVQDESSFQKYLNASREQSDKGYYLD

PNSVNGGLVQERLFDMQVYSWNDNGKANQKTIFYLAGGSYLNNPTPYHISMLKTLSTSLDAKIILPIYPKTPRYTYD

YAIPRLVNLYRHFHEKNANLTLMGDSAGGGLALGLAHALSHQSGQEAIPQPKNIILLSPWLDVTMKHPEIPKYEDTD

PILSAWGLARVGEIWANGSNNTNHTYVSPKNAPATKLAPITLFTGTREIFFPDIRDYAAQLQAANHPVNYIAQEGMN

HVYPIYPIEEAKTAQYQMIDIINKTP

SeqID 477

MTKKHLKTLALALTTVSVVTYSQEVYGLEREESVKQEQTQSASEDDWFEEDNERKTNVSKGNSTVDETVSDLFSDGN

SNNSSSKTESVVSDPKQVPKAKPEVTQEASNSSNDASKVEVPKQDTASKKETIETSTWEAKDFVTRGDTLVGFSKSG

INKLSQTSHLVLPSHAADGTQLTQVASFAFTPDKKTAIAEYTSRLGENGKPSRLDIDQKEIIDEGEIFNAYQLTKLT

IPNGYKSIGQDAFVDNKNIAEVNLPESLETISDYAFAHMSLKQVKLPNLKVVIGELAFFDNQIGGKLYLRRHLIKLA

ERAFKSNRIQTVEFLGSKLKVIGEASFQDNNLSNVMLPDGLEKIESEAFTGNPGDEHYNNQVVLRTRTGQNPHQLAT

ENTYVNPDKSLWRATPDMDYTKWLEEDFTYQKNSVTGFSDKGLQKVRRNKNLEIPKQHNGITITEIGDNAFRNVDFQ

SKTLRKYDLEEVKLPSTIRKIGAFAFQSNNLKSFEASEDLEEIKEGAFMNNRIGTLDLKDKLIKIGDASFHINHIHA

IVLPESVQEIGRSAFRQNGALHLMFIGNKVKTIGEMAFLSNKLESVNLSEQKQLKTIEVQAFSDNALSEVVLPPNLQ

TIREEAFKRRHLKEVKGSSTLSQIAFNAFDQNDGDKRFGKKVVVRTHNNSHMLADGERFIIDPDKLSSTMVDLELVL

KIIEGLDYSTLRQTTQTQFREMITAGKALLSKSNLRQGEKQKFLQEAQFFLGRVDLDKATAKAEKALVTKKATKNGH

LLERSINKAVLAYNNSAIKKANVKRLEKELDLLTDLVEGKGPLAQATMVQGVYLLKTPLPLPEYYIGLNVYFDKSGK

LIYALDMSDTIGEGQKDAYGNPILNVDEDNEGYHALAVATLADYEGLYIKDILNSSLDKIKAIRQIPLAKYHRLGFF

QAIRNAAAEADRLIPKTPKGYLNTVPNYRKKQVEKNSKPVDYKTPIFDKALPNEKVDGDRVAKGHNINAETNNYVAV

TPIRSEQQLHKSQSDVNLPQTSSKNNFIYEILGYVSLCLLFLVTAGKKGKRARK

SeqID 478

MNKKIAGIGLASIAVLSLAACGHRGASKSGGKSDSLKVAMVTDTGGVDDKSFNQSGWEGMQAWGKKNGLKKGAGFDY

FQSASESDYATNLDTAVSSGYKLIFGIGFSLHDAIDKAADNNKDVNYVIVDDVIKGKDNVASVVFADNESAYLAGIA

AAKTTKTKTVGFVGGMESEVITRFEKGFEAGVKSVDKSIKIKVDYAGSFGDAAKGKTIAAAQYASGADIVYQVAGGT

GAGVFSEAKSRNESLKEADKVWVLGVDRDQAAEGKYTSKDGKASNFVLASSIKEVGKSVELIATKTSKGKFPGGNVT

TYGLKDGGVDIATTNLSDDAVKAIKEAKAKIISGDIKVPSK

SeqID 479

MRIQTTNLDKGGGGKSSHTYNEGDWLSRVMGKRVLLIDGARECNLTHSFDVTSDKTIYDIFITGEYEICRINDKLSL

IRGDERLTDEQLDLASRNNKYLQLFMWFSEHYEELAEQFDIILIDTHNDESLVTANCIAVSDIVLGVTDASTNGFRA

WLALKKFVDDIKNEAIEVITKKSYVKAEAYLIGNKIEYYGNNVTETCKQFLDVVQEDEAYLGSIQKKKLMAKSLVIN

QSVFEQREHMTEKQKQDHQKFYDNIEYVYQNILTVLEGETK

SeqID 480

VKKVVFLKILVPLVSLVFLISLGITILAGVLGAVGGSQSNCTIEVVTSTSTDSSISSGDGSIDSFVLEHKEAYILSW

KAGGFLPSASITQTMIENGFNFTNPNGTSLWQAHNMGGVKTSSKSNFPVTLATYGQDSVDLSGTKPGARVGDGTGGA

YTWFSSYDAGIVGKAEFMAHQTLYTKAINNIDGIATLSAIADGGWATDGTYKTKLIDMYHKLGKTYEWLDKEAISAH

GDKPYKATTASAGDAGLSDIFTSNSKDCSDSSSGGATDGTGTVPADAIAWGYRPDNLPESLKQYIIDPKSLGMAYAN

SSGWFNPGSDYLAGQCVNLTISLGNHLWGHSGSVSGNGKDQAGAWANIFGNSVKTTPKRGAIFSTQNGGGGYGHTGI

VCHVFENGSILIVEQNTPLSGADYFKKSYTWNYRIWTPAQQKAEVISFAYPYGKEPKLGN

SeqID 481

MGKTFWKDIYRSITTSKGRFSSILLLMMLGSFAFIGLKVSAPNMQRTAQNYLAHHHVMDITVFNSWGLDKHDQTVLE

SLKGSQVEFSYFVDTTPQQNSKSYRLYSNTKTISTFDLVKGRLPLNKSEIALSFQERKKYAIGDKINFKQDKNKLFS

NTGPLTIVGFVNSTEIWSKTNLGSSQTGDGDLDSYGVLDKTAFHSPVYTMARVTFKDLRLINPFSISYKEKVAKYQE

KVSRKLNIHNKIRYTKTKKESLRKIDEEEKSLLKAQKQINRLDNDSLAMPLSQRQAIQMKIKQDRLSLLKRTKELLK

LRHNTQIMESPQIIVYNRTTFPGGQGYNTFDSSTNSTSKISNLFPIILYLVAALVTLTTMTRFVEEERTNAGILKAL

GYSDRQVIFKFIIYGFIAGTLGTTLGIIGGHYLLPRIISDIISKDLTIPNTQYHLFLNYSLLAFVFSLLSIVLPVFV

ITRRELKEKAAFLLLPKPPAKGSKIALEYINWIWKKLSFTQKVTARNIFRYKQRMIMTIFGVAGSVALLFSGLGIQS

SLKQTVNEHFGRIMPYDILLTYNTNASPPKILELLSKDSKIDKYQPIHLENLDESIPGQINKQSISLFITDKKQLLP

FIYLQEATTNKSLHLNNKGIIISKKLAQFYHVNTGDFIHLSHSQTLPSRKLKITGVVNANVGHYIFMTKQYYRTIFK

KETKDNAFLVKLTKHKIANNLAEKLLEINGVESLTQNALQLASVEAVVRSLDGSMTILVVVSLLLAIVILYNLTNIN

LAERKRELSTIKVLGFYNEEVTLYIYRETIILSTIGVILGTISGTYLHRQMMLLIGSDQILFGEKVSPTTFIIPISV

VVIILISLCFIVNHQLKKLNMLDALKSVD

SeqID 482

MNKRKLLTVLSIASVAVLSSVTFADEITPSDPTAPSTPLITPTPVDPGTPTDTTKPSTPVDPGTPTDTTKPSTPVDP

GTPTDTTKPSTPVDSGTSTNPSDSEATNTNLGTKPTDVVKPETPKIPEVPSTVTPDVIDKVDKETGNITIKPIQISP

DKTIIGTQNGNVIIRDSSGSHLVSASEVGGRLNEDGTVTIKDSSGKTKTLPRTGEAKGFLSIIGATILSFVAYLFKQ

RVTLN

SeqID 483

MRPIVRKKMYKKGKFLVIAGIVTILGGSAILGQDVKAEQAEAATSTISEKTDSSQTISDTSKLTLPVNSSEAMKNSA

EPLIKTGFATSVSSNPREIAATPVKTFDASSKVVVKASTAEHSANQTNSNVNQVANDSEVITQQNSTKQLPKVTYSA

HVQDIGWQKSVDNATVSGTVGQEKQVEAIKLSIKAPEGITGKLSYKTYVKGQGWQPSVESGQVSGTVGQSRPIEALS

INLTDNLQKLYDVYYRVHVQDIGWMAWAKNGAYAGTLGMSKRLEAYEVKFTLKGQSVLTPTILKEERPVLNYQVKVG

QNGWQSNKLEGQMAGTLGESKALDGVKFTLSTLKYGDILYRTHVQDKGWGEILGSQTSKDYQGKRLEAIQLDLSGNL

KQNYDIYYRAHVQDKGWMAWQKNNSVAGTVGESKRLEALEVKLVAKASDFSSQTNHSFLEMKHPGLSYQTYLQKDGW

KPTVLEGQLGGSIGLSKSIKAIKLNLGSTALGNIEYRTFLNGSGWQTVVNSGRESNVPNESQQVEAIQMRLTGLLSK

TYSVMYRVHMQDYGWQDWTYNNDIAGSTNQGKRIEAIEIKLVETAKIPITVQTTYKGTGNYNVRVTNVISPGNLKIA

IWSDKNNQDDLKWYTVTPKNHEARLTFNVTNHRDNGKYFIHVYQENASNQKQLLVKTSLQVAHSNYNTPYFSQRDGR

WASRRYGMATLGETGCVPTSLAMILSSLKGETVLPTQIADYLYHKTVEFNRGVQGTTSRGILKAAKEWGVTATALGT

QANLVQALKDGYHVLAAVQNNVFVLHGSHEIVLKGYNNGLTHVSDPYTPSLSGWYPISQLWKEQSYYSEDRIDIGEP

FVKVTDA

SeqID 484

MARIPYQKTLIFCVAVIIAIFILGLSKDLAQSKGQKVANNNTVKTARVVANGDILLHDVLYASARQPDGTYNFTPYF

KEVKSWIESADLAIGDYEGTISSEYPLAGYPLFNAPNEIATTMKETGYDVVDLAHNHILDSQLAGAINTVKTFNRMG

LDTIGVYLKDRNKEDILIKRVNGIKIAILGYSYGYNGMEANVSKSDYEKHMSDLDTKKIKQDIKKAEKEADITIVMP

QMGIEYQKKPTTEQVMLYHSMIKWGADIIFGGHPHVVEPSEVIKKDGQKKFIIYSMGNFISNQRLETVDDIWTERGL

LMDVTIEKKGQKTVIKKVKAHPTLVEAKANGRYVEEGFPAYDYRTLVLEDYIEGGKKRSTIDQTMKDKVDKAYKEMN

ELVNLKW

SeqID485

MKHFFDYKTMSVARKLSISFIAVILLGSILLSLPIFQYANAPKTHYIDHLFTTVSMVCVTGLSVFPISKVYNGWGQI

VAILLMQTGGLGLVTLMSLSYYTLRRKMSLNDQTLLQSAITYNSSTDLKKYLYMIFKVTLTLEVLAASILAIDFIPR

FGLGHGIFNSIFLAVSAFGNAGFDNLGATSLAQFKLNPLVNIIVCFLIISGGLGFAVWKDLIEATIQTSHKGPKLIK

TFPKRLSNHSKLVLKTTTIILLTGTLLSWLLEFGNFRTIANLSLPKQLMVSFFQTVTMRTAGFSTIDYTQTDFATNL

VYIIQMLIGGAPGGTAGGFKVTVIAILLLLFKAELSGQSQVTFHYRTIPSSIIKQTLSILTFFFIILISGYLLLLEL

NPHIDPFSLFFEASSALATVGVTMNTTNQLSLGGRIVIMFLMFIGRVGPITVLLSILQKKEKEIHYAETEIILG

SeqID 486

MLDELKAIIKSPKLWITMAGVALIPTLYNVIFLSSMWDPYGNTKNLPVAVVNQDKSAKLNGKTISIGKDMEDNLSKN

DSLDFHFTTAKRAEKELEKGHYYMVITFPKDLSRKATTLMTEKPERLNITYKTTKGRSFVASKMSETAANKLKDEVA

ESITGTYTESVFKNMGSMKTGINKAADGSQELLNGSNKLQDGSQTLTSNLDVLASSSQTFSGGANKLNSGINLYTDG

VGTLSNGLETLSDGVTAYTTGVHKLSEGSQKLDDKSQALVEGSEKLTDGLQQLSQATQLKPEQERTLQNLSDGLKNL

NQIITNLQSTATTDSDTNSKLFNFLSTIESSTKALMNTAAADKQKQMTAVQSTSAFKSLTPEQQSQITSAVTGTPTS

AETIAANISSNIENMKTVLSEASSSAPSNNGSQNLQTLSGTANNLVLKAISDLDKIQKLPTATKQLYQGSQTLTKGI

TDYTNAVGQLRKGAVTLDSKSNQLISGTQKASQGAQTLDSKSDQLRDGAGQLASGSDRIADGSNKLAGGGHQLIDGL

TELSGGVSQLSSSLGKAGDQLSMVSVNKDNANAVSSPVTIKHEDYDSVDTNGVGMAPYMISVALMVVALSANVIFAK

ALSGKEPANRFSWAKNKLLINGFIATLAATILFFAVQFIGLKPDYPGKTYFIILLTAWTLMALVTALVGWDNRYGSF

LSLLILLFQLGSSAGTYPIELSPKFFQTIQPFLPMTYSVSGLRETISLTGDVNHQWRMLVIFLVSSMILALLIYRKQ

ED

Claims

1. coding hyper-immuneserum reactive antigen or its segmental isolated nucleic acid molecule, it comprises and is selected from following nucleotide sequence:

A) have the nucleic acid molecule of at least 95% sequence homogeny with the nucleic acid molecule of Seq ID No 147,

B) be complementary to the nucleic acid molecule of nucleic acid molecule a),

C) under stringent hybridization condition, anneal in a) or b) the nucleic acid molecule of nucleic acid molecule,

D) hybridize in a), b) or c) in the degeneracy sequence of nucleic acid molecule of nucleic acid molecule of definition.

2. the isolated nucleic acid molecule of claim 1, wherein the sequence homogeny is 100%.

3. coding hyper-immuneserum reactive antigen or its segmental isolated nucleic acid molecule comprises and is selected from following nucleotide sequence:

A) have 96% or be higher than the nucleic acid molecule of 96% sequence homogeny with the nucleic acid molecule of Seq ID No 147,

B) be complementary to the nucleic acid molecule of nucleic acid molecule a),

4. the isolated nucleic acid molecule of claim 3, wherein said sequence homogeny is 98%.

5. the isolated nucleic acid molecule of claim 3, wherein said sequence homogeny is 100%.

6. the nucleic acid molecule of claim 1, wherein said nucleic acid is DNA.

7. the nucleic acid molecule of claim 1, wherein said nucleic acid is RNA.

8. the isolated nucleic acid molecule of claim 1 is wherein separated described nucleic acid molecule from genomic dna.

9. the isolated nucleic acid molecule of claim 8, wherein said genomic dna is streptococcus agalactiae (S.agalactiae) genomic dna.

10. the carrier that comprises the nucleic acid molecule of claim 1.

11. the carrier of claim 10, wherein said carrier is suitable for the hyper-immuneserum reactive antigen of each nucleic acid molecule encoding in the claim 1 to 9 or it is segmental recombinant expressed through transformation.

12. comprise the host cell of the carrier of claim 10 or 11.

13. comprise the hyper-immuneserum reactive antigen and the fragment thereof of the aminoacid sequence of each nucleic acid molecule encoding among the claim 1-6,8 and 9.

14. the hyper-immuneserum reactive antigen and the fragment thereof of claim 13, wherein said aminoacid sequence are Seq ID No 364.

15. the fragment of hyper-immuneserum reactive antigen, it is selected from the peptide of the aminoacid sequence that comprises the vertical hurdle of table 1A " the immunogenicity amino acid of inferring " and " position in the immunogenicity zone of discriminating ", " amino acid from " of table 2 specified serum reactivity epi-position to " amino acid to " vertical hurdle, and " amino acid from " of table 4 to " amino acid to " indulges specified immunogenicity epi-position hurdle.

16. the fragment of the hyper-immuneserum reactive antigen of claim 15, the wherein said peptide that comprises the aminoacid sequence on the vertical hurdle of table 1A " the immunogenicity amino acid of inferring " and " position in the immunogenicity zone of discriminating " is to comprise following amino acid whose peptide: the 8-36 of Seq ID No 364,40-64,71-79,88-94,102-109,118-127,138-148,151-159,163-174,192-198,200-206,220-233,268-273,290-301,304-309,316-323,331-349,378-391,414-420,427-437,455-475,494-510,541-547,549-555,616-640,1-60,55-139,212-308,386-458 and 458-624.

17. the fragment of the hyper-immuneserum reactive antigen of claim 15, " amino acid from " of wherein said table 2 specified serum reactivity epi-position to " amino acid to " vertical hurdle is to comprise following amino acid whose peptide: 399-417,503-519 and the 544-563 of Seq ID No 364.

18. the fragment of the hyper-immuneserum reactive antigen of claim 15, " amino acid from " of wherein said table 4 specified immunogenicity epi-position to " amino acid to " vertical hurdle is to comprise following amino acid whose peptide: the 489-556 of Seq ID No 364.

19. the segmental method of each streptococcus agalactiae hyper-immuneserum reactive antigen among the streptococcus agalactiae hyper-immuneserum reactive antigen of production claim 13 or 14 or the claim 15-18 comprises the nucleic acid molecule of expressing in the claim 1 to 9 each.

20. the method for the segmental cell of each streptococcus agalactiae hyper-immuneserum reactive antigen among the streptococcus agalactiae hyper-immuneserum reactive antigen of production expression claim 13 or 14 or the claim 15-18 comprises that the carrier with claim 10 or claim 11 transforms or the transfection proper host cell.

21. medicinal compositions comprises in the fragment of each hyper-immuneserum reactive antigen among the hyper-immuneserum reactive antigen of claim 13 or 14 or the claim 15-18 or the claim 1 to 9 each nucleic acid molecule.

22. the pharmaceutical composition of claim 21 is characterised in that it further comprises the immunostimulation material.

23. the pharmaceutical composition of claim 22, wherein said immunostimulation material are selected from polycationic polymer, immunostimulation deoxynucleotide (ODN), the peptide that comprises at least two LysLeuLys motifs, Neurologically-active compounds, alum, Fu Shi is complete or Freund or its combination.

24. the pharmaceutical composition of claim 23, wherein said polycationic polymer are the polycation peptides.

25. the pharmaceutical composition of claim 23, wherein said Neurologically-active compounds is a human growth hormone.

26. each composition among the claim 22-25, wherein said pharmaceutical composition is a vaccine.

27. in the claim 1 to 9 among the hyper-immuneserum reactive antigen of each nucleic acid molecule or claim 13 or 14 or the claim 15-18 fragment of each hyper-immuneserum reactive antigen be used to prepare the purposes of pharmaceutical preparation.

28. the purposes of claim 27, wherein said pharmaceutical preparation are the vaccines that anti-streptococcus agalactiae infects.

29. antibody or its significant part at least, described antibody are at least in conjunction with the segmental selectivity part of each hyper-immuneserum reactive antigen among the hyper-immuneserum reactive antigen of claim 13 or 14 or the claim 15-18.

30. the antibody of claim 29, wherein said antibody is monoclonal antibody.

31. the antibody of claim 29 or 30, wherein said significant part comprises the Fab fragment.

32. the antibody of claim 29, wherein said antibody is chimeric antibody.

33. the antibody of claim 29, wherein said antibody is humanized antibody.

34. hybridoma cell line, it produces at least in conjunction with the antibody of the segmental selectivity part of each hyper-immuneserum reactive antigen among the hyper-immuneserum reactive antigen of claim 13 or 14 or the claim 15-18 or its significant part at least.

35. produce the method for the antibody of claim 29, be characterised in that following step:

● require the fragment of each hyper-immuneserum reactive antigen among 13 or 14 hyper-immuneserum reactive antigen or the claim 15-18 to cause the immune response of described animal by giving inhuman animal right,

● shift out the body fluid that contains antibody from described animal, and

● by being carried out further purification step, the described body fluid that contains antibody produces described antibody.

36. produce the method for the antibody of claim 29, be characterised in that following step:

● shift out spleen or splenocyte from described animal,

● produce the hybridoma of described spleen or splenocyte,

● select and clone the segmental hybridoma that is specific to described hyper-immuneserum reactive antigen or described hyper-immuneserum reactive antigen,

● cultivation and optionally further purification step by described clone hybridization oncocyte produce described antibody.

37. the antibody of claim 29 is used to prepare the purposes for the treatment of or preventing the medicine of streptococcus agalactiae infection.

38. antagonist, it is in conjunction with the fragment of each hyper-immuneserum reactive antigen among the hyper-immuneserum reactive antigen of claim 13 or 14 or the claim 15-18.

39. differentiate and can comprise in conjunction with the method for the segmental antagonist of each hyper-immuneserum reactive antigen among the hyper-immuneserum reactive antigen of claim 13 or 14 or the claim 15-18:

A) in allowing candidate antagonist and isolating or immobilized claim 13 or 14 among each hyper-immuneserum reactive antigen or the claim 15-18 under the fragment bonded condition of each hyper-immuneserum reactive antigen, in the presence of providing, the fragment of described hyper-immuneserum reactive antigen or described hyper-immuneserum reactive antigen is contacted with described candidate antagonist in response to the component of the fragment bonded detectable signal of described candidate antagonist and described hyper-immuneserum reactive antigen or described hyper-immuneserum reactive antigen; And

B) detect in response to described antagonist and described hyper-immuneserum reactive antigen or described hyper-immuneserum reactive antigen segmental combine generation signal existence whether.

40. differentiate the method for the active antagonist of interaction of the fragment of each hyper-immuneserum reactive antigen among the hyper-immuneserum reactive antigen can reduce or suppress claim 13 or 14 or the claim 15-18 and its interacting partner, comprising:

A) provide the fragment of each hyper-immuneserum reactive antigen among the hyper-immuneserum reactive antigen of claim 13 or 14 or the claim 15-18,

B) fragment to described hyper-immuneserum reactive antigen or described hyper-immuneserum reactive antigen provides interacting partner,

C) allow the fragment of described hyper-immuneserum reactive antigen or described hyper-immuneserum reactive antigen and described interacting partner to interact with formation interaction mixture,

D) provide candidate antagonist,

E) allow between candidate antagonist and the interaction mixture competing reaction to take place,

Whether f) measure candidate antagonist suppresses or reduces the fragment of described hyper-immuneserum reactive antigen or described hyper-immuneserum reactive antigen and the interaction activity of interacting partner.

41. the method for claim 40, the fragment of wherein said hyper-immuneserum reactive antigen or described hyper-immuneserum reactive antigen are each antibody among the claim 29-33.

42. the segmental purposes of each hyper-immuneserum reactive antigen among the hyper-immuneserum reactive antigen of claim 13 or 14 or the claim 15-18 is used for separation and/or the purifying and/or the discriminating of the segmental interacting partner of described hyper-immuneserum reactive antigen or described hyper-immuneserum reactive antigen.

43. among the claim 1-9 among the hyper-immuneserum reactive antigen of each nucleotide sequence or claim 13 or 14 or the claim 15-18 fragment of each hyper-immuneserum reactive antigen be used for preparing the relevant disease of each the segmental expression of hyper-immuneserum reactive antigen of the hyper-immuneserum reactive antigen of diagnosis and claim 13 or 14 or claim 15-18 the purposes of medicine.

44. in the claim 1 to 9 among the hyper-immuneserum reactive antigen of each nucleotide sequence or claim 13 or 14 or the claim 15-18 fragment of each hyper-immuneserum reactive antigen be used to prepare the purposes of the medicine of diagnosing infectation of bacteria.

45. being streptococcus agalactiaes, the purposes of claim 44, wherein said infectation of bacteria infect.

46. the segmental purposes of each hyper-immuneserum reactive antigen among the hyper-immuneserum reactive antigen of claim 13 or 14 or the claim 15-18, be used to produce the segmental peptide in conjunction with described hyper-immuneserum reactive antigen or described hyper-immuneserum reactive antigen, wherein said peptide is selected from anti-caline.

47. the fragment of each hyper-immuneserum reactive antigen is used for the purposes that functional nucleic acid is made among the hyper-immuneserum reactive antigen of claim 13 or 14 or the claim 15-18, wherein said functional nucleic acid is selected from fit and specular surface body.

48. each nucleic acid molecule is used to prepare the purposes of functional Yeast Nucleic Acid in the claim 1 to 9, wherein functional Yeast Nucleic Acid is selected from ribozyme, antisense nucleic acid and siRNA.