CN100520394C - Single-channel multi-parameter surface plasms resonance tester - Google Patents

Abstract

The invention discloses a single-channel multi-parameter surface plasmon resonance (SPR) tester, which belongs to the technical field of test instruments. The tester, including the control system, optical system, mechanical scanning system, circulation system, surface plasmon resonance chip, signal acquisition and processing software, and system operation control software, constitutes a complete surface plasmon resonance testing system. The system realizes angle modulation and detects angle and light intensity signals; using two-unit or multi-unit surface plasmon resonance chips, when the sample to be tested flows through the chip through a single channel, the corresponding double-parameter or multi-parameter detection results can be given. The tester of the invention has simple structure and easy operation, can set reference unit detection or multi-parameter automatic test, and the test data is reliable and accurate.

Description

A single-channel multi-parameter surface plasmon resonance tester

technical field

The invention belongs to the technical field of test instruments, in particular to a single-channel multi-parameter surface plasmon resonance (SPR) tester.

Background technique

At present, the single-channel surface plasmon resonance (SPR) tester only measures one parameter at a time, and each channel of the multi-channel surface plasmon resonance (SPR) tester only measures one parameter, resulting in a complicated test process and a large amount of data. Time consuming and error prone.

Contents of the invention

The purpose of the present invention is to propose a novel single-channel multi-parameter Surface Plasmon Resonance (SPR) tester, which adopts two unit chips, cooperates with corresponding optical systems, detection systems and information processing systems to realize dual-parameter detection; or adopts multiple The unit chip and the corresponding signal acquisition and processing system can also realize single-channel multi-parameter testing.

In order to achieve the above object, the technical solution of the present invention is to provide a single-channel multi-parameter surface plasmon resonance tester, including a control system, an optical system, a mechanical scanning system, a circulation system, a surface plasmon resonance chip, and signal acquisition and processing software And the system operation control software and other parts constitute a complete surface plasmon resonance test system, in which, the angle modulation is realized by the mechanical motion scanning structure, and the single-channel detection of the angle and light intensity signal is carried out; it adopts a multi-unit surface plasmon resonance chip, When the sample to be tested flows through the chip through a single channel, the corresponding multi-parameter detection results can be given.

In the surface plasmon resonance tester, the flow system includes a test pool, a pipeline, a liquid inlet valve, a peristaltic pump, and a sensitive chip; wherein, the surface plasmon resonance multi-unit sensitive chip is closely connected under the optical prism. The gold film is closely connected with the test pool under the gold film; the two ends of the test pool are provided with a liquid inlet pipe and a liquid outlet pipe, and the liquid inlet pipe is connected with a liquid inlet valve. The liquid inlet valve has two inlets, one inlet is connected with the sample pipe, The other inlet is connected with the liquid-carrying pipe, and the peristaltic pump is installed on the liquid-carrying pipe; the sample in the measuring cell is in contact with the surface plasmon resonance multi-unit sensitive chip of the circulation system, and the corresponding multi-parameter detection results are given.

In the surface plasmon resonance tester, the surface plasmon resonance chip is a parallel bare gold surface plasmon resonance chip; the multi-unit surface plasmon resonance chip is at least two parallel bare gold surface plasmon resonance chips. Bulk resonator chip.

In the surface plasmon resonance tester, the mechanical scanning system includes an optical platform, a connecting rod group, a slider, a guide rail, a screw, a motor, an angular displacement sensor and a pull rod, wherein the connecting rod group consists of four connecting rods It is composed of four connecting rods forming a rhombus, and the four connecting rods can rotate around the connecting point as the axis. One connecting point B is installed on the optical table, and the other opposite connecting point A is connected to the tie rod, and the other end of the tie rod is connected to the slider. There is a guide rail under the slider, and the slider can move on the guide rail; the other end of the slider is connected with a screw, the other end of the screw is dynamically connected with the motor transmission shaft, and the other end of the screw is connected with the angular displacement sensor;

The light source and the array photodiode are respectively placed on the connecting rods on both sides of the connecting point B of the connecting rod group, and are located on the incident and reflected light paths of the optical prism; the optical prism, surface plasmon resonance chip and measuring cell are combined on the optical platform.

In the surface plasmon resonance tester, the mechanical scanning system can perform an angle test or a light intensity test at a specific angle.

In the surface plasmon resonance tester, the light source is a semiconductor laser; the photodiode array is at least a two-unit photodiode array or CCD.

In the surface plasmon resonance tester, the light source forms a light beam of a certain area through a parallel optical path system; during the dual-parameter test, the optical receiver used is a two-unit photodiode; during the multi-parameter test, the light receiver used The detector is a multi-element photodiode array or CCD.

In the surface plasmon resonance tester, the multi-parameter measurement results given by each measurement, one of the parameters is a reference value, which is compared and differentiated with the sample measurement value, which can eliminate the influence of temperature and noise, and improve the reliability of the test and accuracy.

In the surface plasmon resonance tester, its control system determines the operating speed and direction of the mechanical scanning system according to the operating state, and adjusts the incident angle of the beam with high precision to realize angular scanning; the collected signals are divided into two types: angle and light intensity, When the machine runs to the limit position, the control system corrects the angle, and automatically adjusts the machine speed according to the operation result; at each resolvable incident angle, the system detects the light intensity, and the sampling rate and sample number of detection can be adjusted. Control, the results of each run are used as a reference for determining the next measurement.

The main feature of the present invention is that on the basis of the patent authorized by the inventor: ZL Patent No. 98102366.5, in addition to maintaining the basic structure of the original surface plasmon resonance tester, the system is further expanded and perfected. Generally, a point light source can be used, but the present invention adopts a parallel light beam with a certain area to cover two or more unit chips, so the system of the present invention adopts a parallel light beam light source and two or more unit photodiodes corresponding to the chip and corresponding The flow injection system is equipped with a two-unit or multi-unit chip information collection and processing system. The tester of the invention has simple structure and easy operation, and can do multi-parameter automatic test at the same time, and the test data is reliable and accurate.

Description of drawings

Fig. 1 is the system structural block diagram of single-channel multi-parameter surface plasmon resonance (SPR) tester of the present invention;

Fig. 2 is the optical and mechanical scanning system structural representation of single-channel multi-parameter surface plasmon resonance (SPR) tester of the present invention;

Fig. 3 is a schematic diagram of the system optical path and flow system structure of the single-channel multi-parameter surface plasmon resonance (SPR) tester of the present invention;

Fig. 4 is a test graph of an application example of a single-channel dual-parameter surface plasmon resonance (SPR) tester of the present invention.

Detailed ways

The device of the present invention not only has the unique characteristics of surface plasmon resonance (SPR) biochemical analysis technology: samples do not need to be labeled and purified, can detect turbid or opaque samples, can detect dynamically in real time, have a wide range of incident angles, and have high resolution of angle changes , strong adaptability, etc., and compared with the single-parameter surface plasmon resonance (SPR) tester, it also has high detection sensitivity, less sample volume, convenient and fast process, accurate and reliable, wide application range and high-throughput detection and other advantages, and the device has a supporting flow injection system, which can realize single-channel dual-parameter or multi-parameter automatic detection, and is widely used in life science, chemical analysis, clinical diagnosis, drug screening, food safety, environmental monitoring and related scientific research fields. can be widely applied.

Fig. 1 is a block diagram of the system structure of the present invention, and the composition of tester comprises: optical system, mechanical scanning system, circulation system and data collection system etc. four parts, four parts are connected by standard routine, and with tester control system and computer Connected to form a complete optical, mechanical and electrical integration system.

Fig. 2 is a schematic diagram of the structural principle of the mechanical scanning system. In the figure, tester base 1, optical platform 2, connecting rod group 3, slider 4, guide rail 5, screw rod 6, motor 7, angular displacement sensor 8, pull rod 9, light source 10, two (or more) unit array photoelectric Diode 11 , optical prism 12 and measuring cell 13 .

It is characterized in that a mechanical scanning structure is adopted according to the principle of surface plasmon resonance (SPR) angle modulation. The mechanical scanning motion structure mentioned here includes an optical platform 2, a connecting rod group 3, a slide block 4, a guide rail 5, a screw rod 6, a motor 7, an angular displacement sensor 8 and a pull rod 9, wherein the connecting rod group 3 is composed of four connecting rods The four connecting rods form a rhombus, and the four connecting rods can be rotated as the axis. One connecting point B is installed on the optical table 2, and the other opposite connecting point A is connected to the tie rod 9, and the other end of the tie rod 9 is connected to slider4. There is a guide rail 5 under the slide block 4, and the slide block 4 can move on the guide rail 5. The other end of the slider 4 is connected with a screw 6 , the other end of the screw 6 is dynamically connected with the motor 7 , and the other end of the screw 6 is fixedly connected with the angular displacement sensor 8 .

The light source 10 and two (or more) unit array photodiodes 11 are placed on the connecting rods on both sides of the connecting point B of the connecting rod group 3 respectively, and are located on the incident and reflected light paths of the optical prism 12 . The light source 10 is a semiconductor laser. An optical prism 12 , a surface plasmon resonance (SPR) chip, a measurement cell 13 and the like are combined on the optical platform 2 .

Fig. 3 is a structural schematic diagram of the optical path of the system, two (or more) unit surface plasmon resonance (SPR) chips and the circulation system. Wherein, the gold film 14 of the surface plasmon resonance two-unit sensitive chip 21 or the multi-unit sensitive chip 22 is closely connected under the optical prism 12 , and the test pool 13 is closely connected under the gold film 14 . The two ends of the test tank 13 are provided with a liquid inlet pipe 15 and a liquid outlet pipe 16, and the liquid inlet pipe 15 is connected with a liquid inlet valve 17, and the liquid inlet valve 17 is provided with two inlets, one is connected with the sample pipe 18, and the other is connected with the liquid carrying pipe 19 is communicated, and the peristaltic pump 20 is housed on the carrier liquid pipe 19. The sample in the measuring cell 13 is in contact with the surface plasmon resonance (SPR) two-unit sensitive chip 21 or multi-unit sensitive chip 22 of the circulation system.

Describe the present invention below in conjunction with accompanying drawing.

As shown in Figure 1, the control system determines the operating speed and direction of the mechanical scanning motion structure according to the operating state, and adjusts the incident angle of the beam with high precision to realize angular scanning; the acquisition signal is divided into two types: angle and light intensity. At the limit position, the control system corrects the angle and automatically adjusts the mechanical speed according to the operation result. At each resolvable incident angle, the system detects the light intensity, and the detection sampling rate and number of samples are controllable. The results of each run serve as a reference for determining the next measurement.

It can be seen from Fig. 2 that the lever mechanism can accurately realize the small angular displacement, and the structure is simple. The advantage of this structure is that it can realize a small change in incident angle and a large change range, and at the same time track the reflected light; the sensitivity of the system depends on the minimum resolvable angular displacement of the motor 7 and the angular displacement sensor 8; the accuracy of the system The accuracy is mainly determined by the manufacturing accuracy of the screw rod 6, and the stroke length of the slider 4 and the arm length of the connecting rod determine the size of the measurement range; when using the present invention, the object to be measured is driven by a peristaltic pump and controlled by a valve, and is placed on an optical The measuring cell 13 on the platform 2 contacts and reacts with two-unit or multi-unit sensitive chips of the flow system Surface Plasmon Resonance (SPR), so as to realize the detection of the object to be measured.

Fig. 4 is a curve diagram of actual measured samples when a single-channel dual-parameter surface plasmon resonance (SPR) tester is applied. One of the two curves in the figure (green line) is the reference curve and the other is the sample curve (red line). Comparing the reference value with the measured value of the sample can eliminate the interference and influence of external factors such as temperature and noise, and further Improved test reliability and accuracy.

Claims (5)

1. A single-channel multi-parameter surface plasmon resonance tester, including a control system, an optical system, a mechanical scanning system, a flow system, a surface plasmon resonance chip, signal acquisition and processing software, and system operation control software, forming a complete surface plasmon resonance tester. Plasma resonance test system, in which the mechanical motion scanning structure is used to realize angle modulation, and single-channel detection of angle and light intensity signals; it is characterized in that a multi-unit surface plasmon resonance chip is used, and the multi-unit surface is closely connected under the optical prism The gold film of the plasmon resonance chip is closely connected to the test cell under the gold film; the multi-unit surface plasmon resonance chip is a bare gold surface plasmon resonance chip with at least two units in parallel; parallel beams are used to cover at least two units in parallel The bare gold surface plasmon resonance chip; the light source is a semiconductor laser; the light receiver is an array photodiode or CCD with at least two units; when the sample to be tested flows through the chip through a single channel, the corresponding multi-parameter detection results can be given; The multi-parameter measurement results given by each measurement, one of which is a reference value, is compared with the sample measurement value, which can eliminate the influence of temperature and noise, and improve the reliability and accuracy of the test. 2. The surface plasmon resonance tester according to claim 1, wherein the circulation system includes a test pool, a pipeline, a liquid inlet valve, a peristaltic pump and a sensitive chip; wherein, the two ends of the test pool are provided with The liquid inlet pipe and the liquid outlet pipe, the liquid inlet pipe is connected with the liquid inlet valve, and the liquid inlet valve is provided with two inlets, one inlet is connected with the sample pipe, and the other inlet is connected with the liquid carrying pipe, and the liquid carrying pipe is equipped with a peristaltic pump; test The samples in the cell are in contact with the surface plasmon resonance multi-unit sensitive chip of the circulation system, and the corresponding multi-parameter detection results are given. 3. The surface plasmon resonance tester according to claim 1, wherein the mechanical scanning system includes an optical platform, a connecting rod group, a slide block, a guide rail, a screw, a motor, an angular displacement sensor and a pull rod, wherein , the connecting rod group is composed of four connecting rods. The four connecting rods form a rhombus. The four connecting rods can rotate around the connection point as the axis. One connection point B is installed on the optical table, and the other opposite connection point A is connected to The rod is connected, and the other end of the rod is connected to the slider; there is a guide rail under the slider, and the slider can move on the guide rail; the other end of the slider is connected with a screw, and the other end of the screw is dynamically connected with the drive shaft of the motor, and the other end of the screw is connected to the Angular displacement sensor connection; The light source and the array photodiode are respectively placed on the connecting rods on both sides of the connecting point B of the connecting rod group, and are located on the incident and reflected light paths of the optical prism; the optical prism, surface plasmon resonance chip and test cell are combined on the optical platform. 4. The surface plasmon resonance tester according to claim 3, wherein the mechanical scanning system can perform angle test or light intensity test at a specific angle. 5. The surface plasmon resonance tester according to any one of claims 1, 2, 3 or 4, wherein the control system determines the operating speed and direction of the mechanical scanning system according to the operating state, and adjusts with high precision The incident angle of the beam realizes angle scanning; the collected signal is divided into two types: angle and light intensity. When the machine runs to the limit position, the control system corrects the angle and automatically adjusts the machine speed according to the operation result; The incident angle, the system detects the light intensity, the detection sampling rate and the number of samples are controllable, and the results of each operation are used as a reference for determining the next measurement.

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