CN100495390C - Computer estimation method for salt tolerance of non-halophyte herbaceous plant - Google Patents
Computer estimation method for salt tolerance of non-halophyte herbaceous plant Download PDFInfo
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- 230000015784 hyperosmotic salinity response Effects 0.000 title description 5
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- 238000011156 evaluation Methods 0.000 claims abstract description 20
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Abstract
This invention relates to one non-salt herb's anti-salt property computer evaluation method, which selects non-salt herb plant relative germinating rate, germinating period dry weight, relative water rate, mass film transparent rate, leave Na+/K+(ei), root system Na+/K+(fi) as non-salt herb plant remains adaptive property evaluation index system for adaptive property with different salt content, wherein, the evaluation adopts computer evaluation method with high accordance between evaluation result and field plant result height.
Description
One, technical field
The present invention relates to a kind of computer estimation method, particularly a kind of computer estimation method of non-this plant salt resistant character of salt sward.
Two, background technology
The development and use of salinization soil are the needs of China and even whole world agricultural development.According to the statistics of the World Food Programme and Educational, Scientific and Cultural Organization, there are about 9.5 hundred million hectares of various saliferous clay in the whole world, accounts for 10% of global land surface, is distributed widely in more than 100 countries and regions.Because the secondary salinization of soil, world's saliferous clay area is also in continuous increase.Salination beach areas, strand such as China Huanghe delta have soil entire body salt content height, the degree of mineralization of ground water is big and freshwater resources are deficient characteristics, therefore develop the more landlocked salinization soil of difficulty and want big.Human administer and utilize saliferous clay can be summarized as two kinds of approach, the one, by engineering, chemistry and measure physics, administer and the improvement saliferous clay, make it adapt to the growth of more crops; The 2nd, by further investigation, screen, introduce a fine variety and cultivate new salt-resistant plant to plant anti-salt mechanism, make it adapt to the saliferous clay environment.To utilize the biological control measure of salt-tolerant plant be both economical effective method in screening, the basis that the plant salt resistant character evaluation is the salt-tolerant plant breeding, introduce a fine variety and screen, and the index of evaluation is because of the purpose difference of research difference to some extent.
At Shandong Province's domestic upper soll layer salt content average out to 0.48% of ring Bohai Sea low plain, salt content all has distribution less than 0.1% with soil greater than 4%, differs very greatly different, sees Table 1.Salt content accounts for 17.9% of soil area less than 0.1% non-salinization soil, and the slight salinization soil of salt content 0.1-0.3% accounts for 13.5%, and the salt status of these two kinds of soil is suitable for crop growth, and the area total accounts for 31.4%, how to be developed as the farmland.And soil salt content be 0.3-0.6%, 0.6-0.8% and greater than in 0.8%, severe saliferous clay and solonchak accounts for 68.6%, this part soil is the emphasis that biological modification utilizes.
The low plain chloride type salinization soil classification of the table 1. ring Bohai Sea
Viewpoint biological modification according to " plant adapted soil environment " utilizes salt-affected soil, and the biological salt tolerant power of the plant of being chosen seeds should be greater than 0.4%, just might upgrade vegetation, improvement and utilize large-area salt marsh soil and improve the ecological environment.Develop animal husbandry otherwise pursue kind of a grass simply, do not consider the adaptability of plant, will destroy already very fragile salt marsh ecologic environment.For the utilization in 4,000,000 mu of soil salt content soils about 0.3%, Shandong, in improvement, obtain ecological benefits and economic benefit, promote the development of ring Bohai Sea low plain animal husbandry, therefore selected plant agricultural salt tolerant power should be greater than 0.3%.
The plant salt tolerance ability is the comprehensive embodiment of phytomorph and physiological adaptability, is by the decision of the hereditary capacity of plant.Non-this plant of salt sward comprises gramineous herbaceous plant and leguminous herbaceous plant, and the brine tolerance of gramineous herbaceous plant is stronger than general crops, and is also strong than leguminous herbaceous plant.Gramineous herbaceous plant, especially graminous pasture are the good pionners that improvement utilizes the salt marsh wasteland.Salinity is relevant with composition with the concentration of salinity to the harm of plant, salinity is big more, harmfulness to crop is big more, the murder by poisoning of single salt analogy composite salt is big, in ring Bohai Sea low plain and area, the Huanghe delta, the salt of saliferous clay is grouped into sodium chloride and has comparative advantage, and the ratio of the big more sodium chloride of water-soluble salinity content is also big more.
A lot of with the domestic brine tolerance index and the research of method to plant in the world, be divided into direct evaluation and identify two classes indirectly, China mainly uses the land for growing field crops directly to identify in the past.In recent years, the research that utilizes the physiological and biochemical index of plant to identify indirectly also more appears in the newspapers.Utilize the saline-alkaline tolerance of indoor earth culture or water planting data evaluation plant more convenient, but certain limitation is arranged, nearly all indoor data are all than the high ppt of actual salt resistance ability of plant, yet factual survey has great difficulty on using, because under state of nature, soil salt content is among the variation, is difficult to artificial control, has brought difficulty for the mensuration and the plant salt tolerance ability assessment of soil salt.The comprehensive evaluation appraisal procedure that combines the plant salt resistant character of direct evaluation in land for growing field crops and physiological and biochemical index is not appeared in the newspapers, and does not have non-salt to give birth to the computer estimation method that herbal salt tolerant is identified yet.
Three, summary of the invention
For remedying the deficiencies in the prior art, the present invention combines the achievement in research that the plant salt endurance in more than 20 year on the low plain of the ring Bohai Sea is identified, the computer estimation method of non-this plant salt resistant character of salt sward of a kind of science, practicality is provided.
Non-this plant of salt sward salt marsh adaptability mainly reflects biology and the physiological property of plant, influence the adaptive plant oneself factor of non-this plant of salt sward salt marsh more complicated, and connect each other, interact between each factor, constitute the biochemical ecological method of complex physical jointly.For the assessment of non-this plant of salt sward salt marsh conformability, evaluation index system should be able to reflect non-this plant of salt sward strand salt marsh adaptation level all sidedly, should complement one another between the index, and is well arranged up and down.In order to guarantee the horizontal comparability of index system, should select relative indicatrix for use, simultaneously as relative germination rate and relative dry weight as far as possible.Be applicable to that in order to make up non-salt gives birth to herbal science, comprehensive strand salt marsh adaptability evaluation index system, should follow intension comprehensively, organic assembling, aim that concisely desirable, comparability is strong choose non-this plant of salt sward salt marsh adaptability evaluation index.The concrete index selection principle of the present invention is: (1) scientific principle: index system is wanted to reflect non-this plant of salt sward adaptive essence of strand salt marsh and method and complicacy, this is correct and reflects non-this plant of salt sward strand salt marsh adaptation level objectively, is again prerequisite and the basis that science is determined the plant anti-salt ability.(2) comprehensive principle: this is determined by the adaptive complicacy of non-this plant of salt sward strand salt marsh.Index system wants to reflect non-salt living herbal biology, physiology and biochemical characteristic and mutual relationship thereof.(3) terseness principle: non-this plant of salt sward strand salt marsh adaptability is a very complicated problems, hold its essential characteristic, just must choose representational, active index, is studied thereby constitute succinct index system.The notion of index is wanted clearly, simple and clear easily row, the implication difference between each index, and representative.(4) operability principle: the numerical value of each index should have availability.
The present invention is according to the These parameters selection principle, selected following 6 factors as non-this plant of salt sward strand salt marsh adaptability evaluation index system:
(1) phytobiology proterties: relative germination rate (a
i), seedling stage relative dry weight (b
i), relative moisture content (c
i);
(2) plant physiology proterties: membrane permeability (d
i), Na in the blade
+/ K
+(e
i), root system Na
+/ K
+(f
i).
The preparation method of above-mentioned evaluation index is as follows:
(1) relative germination rate a
iPreparation method:
Be made into 0 (tap water with chemical pure NaCl, do not add NaCl), 0.2%, 0.3%, 0.4%, 0.5%, 0.6%, 0.8% with the salt solusion of 8 different gradients of 1.6%, at bore is to add the sawdust that about 5 grams are rinsed well with tap water in the double dish of 120mm, last lid layer filter paper, the last salt solusion that in each double dish, adds the 40ml preparation respectively, be as the criterion with the sawdust face is equal with liquid level, put into 50 seeds again, spring, summer, autumn, room temperature condition was cultivated down, winter or place incubator, 18 degree 9 hours and 28 degree were alternately cultivated in 15 hours under the alternating temperature condition, observe record chitting piece number every day, in time replenish institute's moisture evaporated, be as the criterion with the sawdust face is equal, make and respectively handle salinity and remain unchanged with liquid level.Each different gradient salt solusion repeats 3 times.Germinate after 10 days, germination percentage is managed in statistic record everywhere.Relative germination rate a
iThe chitting piece that=each different gradient salt solusion are handled under germination seed number/tap water processing is down counted * 100%.
Along with the rising of soil salt content, the germination percentage of equal platymiscium does not all reduce gradually, is exactly the different cultivars of same section, and it is also not of uniform size that its germination percentage reduces amplitude.Gramineae and leguminous forage to the comparison salt tolerant of primary election are done germination test, and except that the hybridization garden sorrel, graminous pasture is than leguminous forage germination percentage height under same salt content condition.According to nearly 20 years beach salty soil district introduction and Experiment result of study, the pulse family clover remains on 70% at salt content 0.3% following germination percentage, and graminous pasture remains on 70% at salt content 0.4% following germination percentage, otherwise herbage germination percentage and survival rate are all lower, sowing quantity big, economic benefit is not remarkable.The herbage variety of therefore suitable certain soil salt content plantation, its germination percentage at least should be more than 70%.
(2) seedling stage relative dry weight b
iWith relative moisture content c
iPreparation method:
Nonporous plastic flowerpot basin with diameter 20cm * high 15cm carries, and every basin is pretended with the air-dry soil of weight, approximately to 4/5 place of flowerpot height, sows about 20 seeds, waters to suitable soil moisture content, is beneficial to seed germination.Each is handled and repeats 3 times.The every basin in back of emerging stays 10 seedlings that growth is even, be evenly distributed, adding chemical pure NaCl by 0 (the no NaCl), 0.2%, 0.3%, 0.4%, 0.5%, 0.6%, 0.8% and 1.6% of soil air dry weight during tri-leaf period carries out salt and handles, NaCl is dissolved in the tap water, impact in order to reduce salt, be incremented to required concentration with 0.3% gradient and water every day.Salt is handled the back and is in time replenished institute's moisture evaporated, and soil moisture content is remained unchanged.Salt was handled 30 days, and results plant overground part is got its fresh weight of half sample weighing, dry weight, calculates dry weight and the water percentage of plant under the different salt divisional processing.All the other samples comprise that root system is used for tenuigenin membrane permeability, blade Na
+/ K
+Na in ratio and the root system
+/ K
+The mensuration of ratio.Relative dry weight b
iPlant dry weight under the=different salt divisional processing/no NaCl handles plant dry weight * 100% down.Moisture relatively c
iPlant water percentage * 100% under plant water percentage under the=different salt divisional processing/no NaCl handles.
Through for many years the relation of living herbal relative dry weight of the non-salt of not equal genus and soil salt content being studied, and the long-term field test research of a large amount of salt tolerant herbages, we find, relative dry weight in plant seedling stage on the saliferous clay remains on more than 70%, just can produce the economic benefit of expection in the production on corresponding salinization soil.
Relative moisture content can become one of leading indicator of weighing the plant salt tolerance ability.Along with the enhancing of condition of salt stress, the physiological drought of plant is more and more serious.Various salt tolerant herbages and non-salt tolerant herbage plant relative moisture content increase with salinity and reduce, and the kind of salt tolerant reduces comparatively slowly relatively, as English ryegrass, Agropyron elongatum, alkali thatch when the soil total salt content 0.6%, the relative moisture content of plant still can remain on more than 80%, and relatively poor plant of brine tolerance such as Holdfast Phalaris grass, orchardgrass are when salt content 0.5%, relative moisture content is lower than 80%, and jaundice and withered appears in its blade.
(3) preparation method of plant physiology index
Tenuigenin membrane permeability d
iPreparation method: the exosmose conductivity of material of the cell of measuring plant sample under the different salt marsh conditions with electrical conductivity method, weigh the size of tenuigenin membrane permeability successively.Get on the plant in the above-mentioned test functional leaf of certain leaf age and level, wrap in the wet gauze and fetch, with the clean back of flushing with clean water with distilled water flushing, thieving paper suck dry moisture after, shred into 1cm
2Size takes by weighing 1 this sample of gram, adds 20ml distilled water, bleeds 30 minutes.All processing are all the same, repeat 3 times.Then sample is put into vacuum dryer, vacuum pump is bled 3-4 time, keeps 30 minutes at every turn, and the 20-30 degree was placed 2 hours down.To contrast and directly place boiling water bath heating 15 minutes, kill tissues, the loam cake glass plate prevents moisture loss during water-bath.After the cooling, measure electrical conductivity of solution on the conductivity meter.Phyteral membrane permeability d
i(%)=sample conductivity/contrast conductivity * 100%.
The phyteral membrane permeability is an important physical signs of vegetable cell physiological function, coerces down at salinity, and plant plasma membrane serves at first comes to harm, and causes the saturating property of its selectivity destroyed, saturating property increase.Coerce down at identical salinity, the salt resistance of plants that the tenuigenin membrane permeability is big is little, and the little salt-resistance of membrane permeability is big.According to the test result of a large amount of herbage salt stress tests and tenuigenin membrane permeability thereof, when the electrolyte rate of exosmosing reached 60%, overground part shows the salt damage symptom: plant was short and small, and Lao Ye is dead in advance.Therefore its turbine blades in electrochemical matter of non-halophytes of salt tolerant exosmose percent should be less than 60%.
Na in the blade
+/ K
+Compare e
i, root system Na
+/ K
+Compare f
iPreparation method: take by weighing 50 milligrams of root system and leaf samples after the oven dry respectively, in carbonization treatment on the electric furnace to do not have white cigarette emit do well after, 550 degree ashing are 4 hours in Ma Fulu, a little nitric acid dissolve of vacuum dryer cooling back, deionized water is settled to 100ml, uses the K in the flame spectrophotometer measuring solution then
+, Na
+Content calculates Na in the root system respectively
+/ K
+Na in ratio and the blade
+/ K
+Ratio.
Na in plant leaf blade and the root system
+Content increases along with the increase of salt content in the soil, and K
+Content then reduces along with the increase of salt content in the soil, so plant leaf blade and root system Na
+/ K
+Increase along with the increase of salt stress concentration than all, this has reflected from root system to the overground part blade K
+Selectivity more and more lower, and to Na
+Absorption more and more, be deposited in the Na in root system and the blade
+More and more.
According to a large amount of field tests and lab analysis result, we find Na in non-this plant leaf blade of salt sward of salt tolerant
+/ K
+Compare greater than 0.7 Na in the root system
+/ K
+Ratio was greater than 0.9 o'clock, and plant shows tangible salt damage symptom.Therefore with Na in the blade
+/ K
+Than less than 0.7 and root system in Na
+/ K
+Ratio is the critical point of salt tolerant index less than 0.9.
The computer estimation method of non-this plant salt resistant character of salt sward is as follows:
1), starts computing machine and initialization;
2), different gradient salt solusions or the upper soll layer total salt content that NaCl is set is: 0% (contrast), 0.2%, 0.3%, 0.4%, 0.5%, 0.6%, 0.8%, 1.6%; Variable and assignment: a=0, b=0, c=0, d=0, e=0, f=0, x=0 are set, and x is the short slab value;
3), input botanical name;
4), Na in input relative germination rate, seedling stage relative dry weight, relative moisture content, phyteral membrane permeability, the blade
+/ K
+Na in ratio and the root system
+/ K
+Than the measured value of evaluation index, 8 data of every group respectively assignment be a
i, b
i, c
i, d
i, e
i, f
i, wherein, i=1,2,3 ... 8;
5), read relative germination rate a
i, and compare relative germination rate under different gradient salt solusions or the upper soll layer total salt content condition successively, if a
i＜70%, comparison finishes; If a
i〉=70%, then establish a=a+1, proceed comparison;
6), read relative dry weight b in seedling stage
i, and compare relative dry weight under different gradient salt solusions or the upper soll layer total salt content condition successively, if b
i＜70%, comparison finishes; If b
i〉=70%, then establish b=b+1, proceed comparison;
7), read relative moisture content c
i, and compare relative moisture content under different gradient salt solusions or the upper soll layer total salt content condition successively, if c
i＜80%, comparison finishes; If c
i〉=80%, then establish c=c+1, proceed comparison;
8), read membrane permeability d
i, and compare membrane permeability under different gradient salt solusions or the upper soll layer total salt content condition successively, if d
i60%, comparison finishes; If d
i≤ 60%, then establish d=d+1, proceed comparison;
9), read Na in the blade
+/ K
+e
i, and compare Na in different gradient salt solusions or the upper soll layer total salt content condition lower blade successively
+/ K
+If, e
i0.7%, comparison finishes; If e
i≤ 0.7%, then establish e=e+1, proceed comparison;
10), read Na in the root system
+/ K
+f
i, and compare under different gradient salt solusions or the upper soll layer total salt content condition Na in the root system successively
+/ K
+If, f
i0.9%, comparison finishes; If f
i≤ 0.9%, then establish f=f+1, proceed comparison;
11), get the minimum value among a, b, c, d, e, the f, this value is short slab value x; X judges according to this short slab value: x=0, and this kind is not suitable for introducing a fine variety; X=1, this kind salt tolerant limit is 0.2%, suitable slight salinization soil; X=2, this kind salt tolerant limit is 0.3%, suitable slight salinization soil; X=3, this kind salt tolerant limit is 0.4%, suitable moderate salinization soil; X=4, this kind salt tolerant limit is 0.5%, suitable moderate salinization soil; X=5, this kind salt tolerant limit is 0.6%, suitable moderate salinization soil; X=6, this kind salt tolerant limit is 0.8%, suitable severe salinization soil; X=7, this kind salt tolerant limit is 1.6% or greater than 1.6%, suitable solonchak soil.
Test shows, compares with existing non-this plant salt tolerance of salt sward authenticate technology, and the present invention has following advantage:
(1) evaluation index is easy to acquisition, workable.Obtain the seed relative germination rate by indoor germination test, potted plant acquisition plant relative dry weight in seedling stage, other index all can obtain in common agricultural fractional analysis laboratory.
(2) evaluation system methodization, computer programing.This is invented first herbal salt resistant character evaluation index methodization, and has analyzed the weight of each index, and the evaluation process computer programing.
(3) assessment period shortens over half.The about 45 day time of whole evaluation process can finish, and assesses plant salt resistant character than open-air field production mode and saves the time over half.
(4) wide adaptability.This appraisal procedure is based upon on sulfate-sodium chloride type coast saline soil basis, can be applied on sodium chloride-sulfate type landlocked saline soil.
(5) economize on resources.At first save sowing quantity, save the field test land used, and the collection of different salt content soil, analysis and landfill are put in order processes such as ground.
(6) risk is introduced a fine variety in minimizing.Height is consistent as a result with open-air field planting for assessment result, greatly reduces the risk of saline soil area plant introduction, screening and breeding.
Four, description of drawings
Fig. 1 is a computer program process flow diagram of the present invention.
Five, embodiment
Embodiment 1: the brine tolerance of Martin's alta fascue (Festuca Arundinacea) is identified
1, materials and methods
Introduce a fine variety kind: Martin's alta fascue (Festuca Arundinacea)
Test method: according to Martin's alta fascue characteristic introduction of introducing a fine variety, adopt high salinity 0.8% to carry out hot-house culture and pot experiment, and carry out necessary lab analysis.
(1) percentage of seedgermination hot-house culture test: with chemical pure NaCl be made into 0 (not adding NaCl), 0.2%, 0.3%, 0.4%, 0.5%, 0.6% with the salt solusion of 0.8% 7 different gradients, at diameter is to add the sawdust that about 5 grams are cleaned in the double dish of 120mm, last lid layer filter paper, the salt solusion that in each double dish, adds about 40ml preparation respectively, put into 50 seeds again, place incubator, 18 degree 9 hours and 28 degree were alternately cultivated in 15 hours under the alternating temperature condition.In time replenish institute's moisture evaporated between culture period, be as the criterion with the sawdust face is equal, make and respectively handle salinity and remain unchanged with liquid level.Each different gradient salt solusion repeats 3 times.Germinate after 10 days, germination percentage is managed in statistic record everywhere.Calculate relative germination rate: a
iThe chitting piece that=different gradient salt solusions are handled under germination seed number/tap water processing is down counted * 100%.
(2) pot experiment: with the nonporous plastic basin of diameter 20cm * high 15cm, every basin is adorned native 2.5kg, sows about 20 seeds, waters to suitable soil moisture content.Each is handled and repeats 3 times.10 seedlings that the every basin in back of emerging stays growth, is evenly distributed, time-division in tri-leaf period another name are got 0 (no NaCl), 5,7.5,10,12.5,15 and 20 gram chemical pure NaCl, respectively salt are carried out in test and handle.NaCl is dissolved in the tap water, and every day, the gradient with 0.3% was incremented to required concentration, made soil salt content reach 0,0.2%, 0.3%, 0.4%, 0.5%, 0.6% and 0.8% respectively.Growing period in time replenishes institute's moisture evaporated, and soil moisture content is remained unchanged.Salt was handled 30 days, results plant overground part.
(3) lab analysis: take by weighing its fresh weight of half sample weighing, put into baking oven 105 degree and completed 30 minutes, 80 degree are dried to constant weight, weigh, and calculate relative dry weight and the relative moisture content of plant under the different salt divisional processing.All the other samples comprise that root system is used for tenuigenin membrane permeability, blade Na
+/ K
+Na in ratio and the root system
+/ K
+The mensuration of ratio.
The mensuration of tenuigenin membrane permeability: get the medium leaf age of plant in the above-mentioned test and the functional leaf of level, wrap in the wet gauze and fetch, behind, the thieving paper suck dry moisture clean, shred into 1cm with distilled water flushing
2Size takes by weighing 1 this sample of gram, adds 20ml distilled water, bleeds 30 minutes.All processing are all the same, repeat 3 times.Then sample is put into vacuum dryer, vacuum pump is bled 3-4 time, keeps 30 minutes at every turn, places 2 hours under the room temperature.To contrast and directly place boiling water bath heating 15 minutes, kill tissues, loam cake glass plate during water-bath.After the cooling, measure electrical conductivity of solution on the conductivity meter.Electrolyte percent (the %)=sample conductivity/contrast conductivity * 100% that exosmoses.
Na in the blade
+/ K
+Ratio, root system Na
+/ K
+The mensuration of ratio: take by weighing 50 milligrams of root system and leaf samples after the oven dry respectively, in carbonization treatment on the electric furnace to do not have white cigarette emit do well after, 550 degree ashing are 4 hours in Ma Fulu, a little nitric acid dissolve of vacuum dryer cooling back, deionized water is settled to 100ml, uses the K in the flame spectrophotometer measuring solution then
+, Na
+Content calculates Na in the root system respectively
+/ K
+Na in ratio and the blade
+/ K
+Ratio.
2, test findings
Each brine tolerance identification of indicator of Martin's alta fascue the results are shown in Table 2.
Table 2. Martin alta fascue brine tolerance identification of indicator result
The test findings that Martin's alta fascue brine tolerance is identified is imported " non-this plant salt resistant character of salt sward appraisal procedure ", the assessment result that obtains this plant after this computer approach assessment is: " this kind salt tolerant limit is 0.6%, suitable moderate salinization soil." this assessment result is identical with the result of plantation Martin alta fascue brine tolerance test in the salinization soil of land for growing field crops.
Embodiment 2: the brine tolerance of Australia bit Agropyron elongatum is identified
1, materials and methods
Introduce a fine variety kind: Australia bit Agropyron elongatum
Test method: (1) percentage of seedgermination test: with chemical pure NaCl be made into 0 (not adding NaCl), 0.2%, 0.3%, 0.4%, 0.5%, 0.6%, 0.8% with the salt solusion of 1.6% 8 different gradients, at diameter is to add the sawdust that about 5 grams are cleaned in the double dish of 120mm, last lid layer filter paper, the salt solusion that in each double dish, adds about 40ml preparation respectively, put into 50 seeds again, place incubator, 18 degree 9 hours and 28 degree were alternately cultivated in 15 hours under the alternating temperature condition.In time replenish institute's moisture evaporated between culture period, be as the criterion with the sawdust face is equal, make and respectively handle salinity and remain unchanged with liquid level.Each different gradient salt solusion repeats 3 times.Germinate after 10 days, germination percentage is managed in statistic record everywhere, and calculates relative germination rate.
(2) pot experiment: with the nonporous plastic basin of diameter 20cm * high 15cm, every basin is adorned native 2.5kg, sows about 20 seeds, waters to suitable soil moisture content.Each is handled and repeats 3 times.10 seedlings that the every basin in back of emerging stays growth, is evenly distributed, time-division in tri-leaf period another name are got 0 (no NaCl), 5,7.5,10,12.5,15,20 and 40 gram chemical pure NaCl, respectively salt are carried out in test and handle.NaCl is dissolved in the tap water, and every day, the gradient with 0.3% was incremented to required concentration, made soil salt content reach 0,0.2%, 0.3%, 0.4%, 0.5%, 0.6%, 0.8% and 1.6% respectively.Growing period in time replenishes institute's moisture evaporated, makes soil moisture content keep stable.Salt was handled 30 days, results plant overground part.
(3) lab analysis: take by weighing its fresh weight of half sample weighing, put into baking oven 105 degree and completed 30 minutes, 80 degree are dried to constant weight, weigh, and calculate dry weight, the water percentage of plant under the different salt divisional processing, and calculate relative dry weight and relative moisture content.All the other samples comprise that root system is used for tenuigenin membrane permeability, blade Na
+/ K
+Na in ratio and the root system
+/ K
+The mensuration of ratio.
The mensuration of tenuigenin membrane permeability: get the medium leaf age of plant in the above-mentioned test and the functional leaf of level, wrap in the wet gauze and fetch, behind, the thieving paper suck dry moisture clean, shred into 1cm with distilled water flushing
2Size takes by weighing 1 this sample of gram, adds 20ml distilled water, bleeds 30 minutes.All processing are all the same, repeat 3 times.Then sample is put into vacuum dryer, vacuum pump is bled 3-4 time, keeps 30 minutes at every turn, places 2 hours under the room temperature.To contrast and directly place boiling water bath heating l5 minute, kill tissues, loam cake glass plate during water-bath.After the cooling, measure electrical conductivity of solution on the conductivity meter.Electrolyte percent (the %)=sample conductivity/contrast conductivity * 100% that exosmoses.
Na in the blade
+/ K
+Ratio, root system Na
+/ K
+The mensuration of ratio: take by weighing 50 milligrams of root system and leaf samples after the oven dry respectively, in carbonization treatment on the electric furnace to do not have white cigarette emit do well after, 550 degree ashing are 4 hours in Ma Fulu, a little nitric acid dissolve of vacuum dryer cooling back, deionized water is settled to 100ml, uses the K in the flame spectrophotometer measuring solution then
+, Na
+Content calculates Na in the root system respectively
+/ K
+Na in ratio and the blade
+/ K
+Ratio.
2, test findings
Australia's bit Agropyron elongatum brine tolerance identification of indicator measurement result sees Table 3.
Table 3. Australia bit Agropyron elongatum brine tolerance identification of indicator result
The test findings that Australia's bit Agropyron elongatum brine tolerance is identified is imported " non-this plant salt resistant character of salt sward appraisal procedure ", the assessment result that obtains this plant after this computer approach assessment is: " this kind salt tolerant limit is 0.8%, suitable severe salinization soil." height is consistent as a result to introduce a fine variety the brine tolerance design test of Australia bit Agropyron elongatum in this assessment result and the strand salinization soil.
The brine tolerance of embodiment 3:Holdfast Phalaris grass is identified
1, materials and methods
Introduce a fine variety kind: Holdfast Phalaris grass
Test method:, adopt high salinity 0.6% to carry out percentage of seedgermination test and pot experiment, and carry out necessary lab analysis according to the breediness introduction of the Holdfast Phalaris grass of introducing a fine variety.
(1) percentage of seedgermination test: with chemical pure NaCl be made into 0 (not adding NaCl), 0.2%, 0.3%, 0.4%, 0.5% with the salt solusion of 0.6% 6 different gradients, at diameter is to add the sawdust that about 5 grams are cleaned in the double dish of 120mm, last lid layer filter paper, the salt solusion that in each double dish, adds about 40ml preparation respectively, put into 50 seeds again, place incubator, 18 degree 9 hours and 28 degree were alternately cultivated in 15 hours under the alternating temperature condition.In time replenish institute's moisture evaporated between culture period, be as the criterion with the sawdust face is equal, make and respectively handle salinity and remain unchanged with liquid level.Each different gradient salt solusion repeats 3 times.Germinate after 10 days, germination percentage is managed in statistic record everywhere.The chitting piece that calculates under germination seed number/tap water is handled under the different gradient salt solusions processing of relative germination rate: ai=is counted * 100%.。
(2) pot experiment: with the nonporous plastic basin of diameter 20cm * high 15cm, every basin is adorned native 2.5kg, sows about 20 seeds, waters to suitable soil moisture content.Each is handled and repeats 3 times.10 seedlings that the every basin in back of emerging stays growth, is evenly distributed, time-division in tri-leaf period another name are got 0 (no NaCl), 5,7.5,10,12.5 and 15 gram chemical pure NaCl, respectively salt are carried out in test and handle.NaCl is dissolved in the tap water, and every day, the gradient with 0.3% was incremented to required concentration, made soil salt content reach 0,0.2%, 0.3%, 0.4%, 0.5% and 0.6% respectively.Growing period in time replenishes institute's moisture evaporated, and soil moisture content is remained unchanged.Salt was handled 30 days, results plant overground part.
(3) lab analysis: take by weighing its fresh weight of half sample weighing, put into baking oven 105 degree and completed 30 minutes, 80 degree are dried to constant weight, weigh, and calculate relative dry weight and the relative moisture content of plant under the different salt divisional processing.All the other samples comprise that root system is used for tenuigenin membrane permeability, blade Na
+/ K
+Na in ratio and the root system
+/ K
+The mensuration of ratio.
The mensuration of tenuigenin membrane permeability: get the medium leaf age of plant in the above-mentioned test and the functional leaf of level, wrap in the wet gauze and fetch, behind, the thieving paper suck dry moisture clean, shred into 1cm with distilled water flushing
2Size takes by weighing 1 this sample of gram, adds 20ml distilled water, bleeds 30 minutes.All processing are all the same, repeat 3 times.Then sample is put into vacuum dryer, vacuum pump is bled 3-4 time, keeps 30 minutes at every turn, places 2 hours under the room temperature.To contrast and directly place boiling water bath heating 15 minutes, kill tissues, the loam cake glass plate prevents moisture loss during water-bath.After the cooling, measure electrical conductivity of solution on the conductivity meter, calculate the electrolyte percent that exosmoses.
Na in the blade
+/ K
+Ratio, root system Na
+/ K
+The mensuration of ratio: take by weighing 50 milligrams of root system and leaf samples after the oven dry respectively, in carbonization treatment on the electric furnace to do not have white cigarette emit do well after, 550 degree ashing are 4 hours in Ma Fulu, a little nitric acid dissolve of vacuum dryer cooling back, deionized water is settled to 100ml, uses the K in the flame spectrophotometer measuring solution then
+, Na
+Content calculates Na in the root system respectively
+/ K
+Na in ratio and the blade
+/ K
+Ratio.
2, test findings
Holdfast Phalaris grass brine tolerance identification of indicator measurement result sees Table 4.
Table 4. Phalaris grass brine tolerance identification of indicator result
The brine tolerance design test result of Phalaris grass input " non-this plant salt resistant character of salt sward appraisal procedure ", the assessment result that obtains this plant after this computer approach assessment is: " this kind salt tolerant limit is 0.3%, suitable slight salinization soil." this assessment result is in full accord with the brine tolerance test findings of plantation Holdfast Phalaris grass in the salinization soil of strand.
Claims (1)
1, a kind of computer estimation method of non-this plant salt resistant character of salt sward is characterized in that, the method for concrete assessment is as follows:
1), starts computing machine and initialization;
2), different gradient salt solusions or the upper soll layer total salt content that NaCl is set is: 0% (contrast), 0.2%, 0.3%, 0.4%, 0.5%, 0.6%, 0.8%, 1.6%; Variable and assignment: a=0, b=0, c=0, d=0, e=0, f=0, x=0 are set, and x is the short slab value;
3), input botanical name;
4), Na in input relative germination rate, seedling stage relative dry weight, relative moisture content, phyteral membrane permeability, the blade
+/ K
+Na in ratio and the root system
+/ K
+Than the measured value of evaluation index, 8 data of every group respectively assignment be a
i, b
i, c
i, d
i, e
i, f
i, wherein, i=1,2,3 ... 8;
5), read relative germination rate a
i, and compare relative germination rate under different gradient salt solusions or the upper soll layer total salt content condition successively, if a
i＜70%, comparison finishes; If a
i〉=70%, then establish a=a+1, proceed comparison;
6), read relative dry weight b in seedling stage
i, and compare relative dry weight under different gradient salt solusions or the upper soll layer total salt content condition successively, if b
i＜70%, comparison finishes; If b
i〉=70%, then establish b=b+1, proceed comparison;
7), read relative moisture content c
i, and compare relative moisture content under different gradient salt solusions or the upper soll layer total salt content condition successively, if c
i＜80%, comparison finishes; If c
i〉=80%, then establish c=c+1, proceed comparison;
8), read membrane permeability d
i, and compare membrane permeability under different gradient salt solusions or the upper soll layer total salt content condition successively, if d
i60%, comparison finishes; If d
i≤ 60%, then establish d=d+1, proceed comparison;
9), read Na in the blade
+/ K
+e
i, and compare Na in different gradient salt solusions or the upper soll layer total salt content condition lower blade successively
+/ K
+If, e
i0.7%, comparison finishes; If e
i≤ 0.7%, then establish e=e+1, proceed comparison;
10), read Na in the root system
+/ K
+f
i, and compare under different gradient salt solusions or the upper soll layer total salt content condition Na in the root system successively
+/ K
+If, f
i0.9%, comparison finishes; If f
i≤ 0.9%, then establish f=f+1, proceed comparison;
11), get the minimum value among a, b, c, d, e, the f, this value is short slab value x; X judges according to this short slab value: x=0, and this kind is not suitable for introducing a fine variety; X=1, this kind salt tolerant limit is 0.2%, suitable slight salinization soil; X=2, this kind salt tolerant limit is 0.3%, suitable slight salinization soil; X=3, this kind salt tolerant limit is 0.4%, suitable moderate salinization soil; X=4, this kind salt tolerant limit is 0.5%, suitable moderate salinization soil; X=5, this kind salt tolerant limit is 0.6%, suitable moderate salinization soil; X=6, this kind salt tolerant limit is 0.8%, suitable severe salinization soil; X=7, this kind salt tolerant limit is 1.6% or greater than 1.6%, suitable solonchak soil.
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| CN102511325B (en) * | 2011-12-26 | 2013-10-16 | 杭州市园林绿化股份有限公司 | Method for sieving salt-tolerant varieties of osmanthus fragrans |
| CN108781850A (en) * | 2017-05-05 | 2018-11-13 | 江苏溯源农业科技发展有限公司 | A kind of Salt-Tolerance Identification method of the black China fir of hybridization |
| CN109105235A (en) * | 2018-08-17 | 2019-01-01 | 河北省农林科学院昌黎果树研究所 | A kind of identification method of grape rootstock salt tolerance |
| CN109699489A (en) * | 2019-01-18 | 2019-05-03 | 南通大学 | It polymerize the willow breeding method of salt tolerant, fast-growing character |
| CN112051363B (en) * | 2020-09-02 | 2023-07-14 | 西南民族大学 | A method for judging the degree of alpine meadow degradation based on root-to-soil ratio |
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| WO2006025060A2 (en) * | 2004-09-02 | 2006-03-09 | Yeda Research And Development Co. Ltd. At The Weizmann Institute Of Science | Salt responsive genes useful for generating salt resistant transgenic plants |
| CN1793083A (en) * | 2005-11-30 | 2006-06-28 | 冷亮 | Preparation method of temperature-resistant salt-resistant composite flower soil |
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| WO2006025060A2 (en) * | 2004-09-02 | 2006-03-09 | Yeda Research And Development Co. Ltd. At The Weizmann Institute Of Science | Salt responsive genes useful for generating salt resistant transgenic plants |
| CN1793083A (en) * | 2005-11-30 | 2006-06-28 | 冷亮 | Preparation method of temperature-resistant salt-resistant composite flower soil |
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| 土壤盐渍化与作物耐盐性模型研究与应用. 朱美玲，刘国勇.新疆农业科学，第40卷第S2期. 2003 * |
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