CN100451185C - Method for synthesizing magnetic nano crystal material from nematode - Google Patents

Abstract

The invention discloses a method for synthesizing magnetic nano crystal materials by nematodes, which is characterized in that the synchronized nematode C.elegans is inoculated into the s-media culture solution containing Escherichia coli E.coli solution, and the Escherichia coli solution Contain magnetic metal compounds or add magnetic metal compounds to the s-media culture medium. After culturing and breeding, use ultrapure water to clean the remaining culture medium and E. coli on the surface of nematodes, and put them into small acid-washed test tubes Freeze-dried, nematode bodies contain magnetic nanocrystals. The magnetic nano crystal synthesized by the invention will not cause environmental pollution and has high biocompatibility.

Description

Method for Synthesizing Magnetic Nanocrystalline Materials in C. elegans

technical field

The invention belongs to a method for biosynthesizing materials, in particular to a method for synthesizing magnetic nano crystals by using organisms.

Background technique

In terms of the preparation of magnetic nanocrystalline materials, the latest development is the high-temperature thermal decomposition preparation method. By selecting a strong polar solvent with a high boiling point α-pyrrolidone as the reaction heat transfer medium, a "one-pot" reaction is used to prepare a material with high crystallinity and Water-soluble magnetic Fe ₃ O ₄ nanocrystals with high magnetic properties. Although a large amount of magnetic nanomaterials can be prepared by using this method, a large amount of HCl is produced in the reaction at the same time, which will cause environmental pollution and consume a large amount of energy. The recently developed PEG surface modification technology has improved the biocompatibility of magnetic nanocrystals to a certain extent, but it is still difficult to compare with materials synthesized by organisms themselves.

Contents of the invention

In order to overcome the environmental non-friendliness and biological incompatibility of the existing chemical method for preparing magnetic nano crystals, the present invention proposes a method for nematodes to synthesize magnetic nano crystal materials.

Technical scheme of the present invention is as follows:

The method for nematode synthetic magnetic nano crystal material is characterized in that the synchronized nematode C.elegans will be inoculated into the s-media culture fluid containing Escherichia coli E.coli liquid, and the E. coli liquid contains magnetic metal compounds or in Add magnetic metal compounds to the s-media culture solution. After culturing and breeding, use ultrapure water to wash the remaining culture solution and E. coli on the surface of the nematode body, put it into an acid-washed small test tube to freeze-dry, and put it in the nematode body Contains magnetic nanocrystals.

The magnetic metal compound refers to ferroferric oxide magnetic powder. The time for cultivating and propagating is to cultivate at 20°C for 4 days. The magnetic nanocrystals were collected by gradient centrifugation under the action of an electromagnetic field of 500 mT.

The nematodes are replaced by wild-type plants and their exogenous bodies, transgenic plants and their exogenous bodies, microorganisms, nematodes, mammals and their cells.

The present invention utilizes the self-organization ability of organism C.elegans to synthesize γFe ₂ O ₃ , Fe ₃ O ₄ or Fe ₃ S ₄ molecules from the absorbed Fe ²⁺ and Fe ³⁺ , and crystallize the C. .elegans cracking, and magnetic nanocrystals were obtained after gradient centrifugation in a magnetic field.

Due to the rapid reproduction of C.elegans, under ideal conditions

n=n ₀ 100 ^t/3

Among them, n is the number of insect population on day t; n ₀ is the initial number of insect population

Therefore, the speed of using organisms to synthesize magnetic nanocrystals is considerable, and will not cause environmental pollution, and has high biocompatibility.

Detailed ways

Inoculate the nematode C.elegans that has been synchronized (meaning that the age and development level are basically the same) into the s-media culture solution containing E. coli E.coli solution (which contains ferric oxide magnetic powder), and after culturing at 20°C for 4 days, Wash the nematode body surface 6 times with ultrapure water to remove residual culture medium and E.coli solution. Put it into a small test tube washed with acid and freeze-dry it. Use the freeze-drying technique to crystallize the γFe ₂ O ₃ , Fe ₃ O ₄ or Fe ₃ S ₄ molecules in the nematode body at a frequency of 1000Mhz and a magnetic field strength of 100-500mT Under the action of an electromagnetic field, the nematode body is gradiently centrifuged to collect magnetic nanocrystals.

The present invention can also use wild-type plants and their exogenous bodies, transgenic plants and their exogenous bodies, microorganisms, nematodes, mammals and their cells to replace nematodes to biosynthesize magnetic nanocrystals, and the process conditions are the same.

Magnetic nanocrystals can also be collected using methods such as electric field, magnetic field, crushing, and adsorbent.

Claims (3)

1. the method for synthesizing magnetic nano crystal material from nematode, it is characterized in that will be being seeded in the s-media nutrient solution that contains intestinal bacteria E.coli liquid through synchronized nematode C.elegans, contain the magneticmetal compound in its intestinal bacteria liquid or in the s-media nutrient solution, add the magneticmetal compound, after cultivating breeding, use ultrapure water Cleaning Line polypide remaining nutrient solution and the intestinal bacteria E.coli in surface, put into small test tube freeze-drying through pickling, contain magnetic nano crystal in the nematode body, obtain magnetic nano crystal in the magnetic field after the gradient centrifugation.

2. method according to claim 1, it is characterized in that described magneticmetal compound is meant ferroferric oxide magnetic powder, the time of described cultivation breeding is to cultivate 4 days down at 20 ℃, the nematode body is in small test tube after the freeze-drying, in frequency is 1000Mhz, magneticstrength is a gradient centrifugation under the electromagnetic field effect of 100-500mT, collects and obtains magnetic nano crystal.

3. method according to claim 1, it is characterized in that described nematode by wild-type plant with and grow body, transgenic plant outward and grow body, microorganism, nematode, Mammals outward and cell replaces.