CN100425143C - Seed coating agent for preventing cotton from fusarium / verticillium wilt - Google Patents

Abstract

The invention belongs to a pesticide, in particular to a seed coating agent for resisting cotton fusarium wilt and verticillium wilt, which is made by using natural abscisic acid and Bacillus subtilis ZK-3. According to the current research on abscisic acid and Bacillus subtilis ZK-3, the present invention discloses a seed coating agent for resisting cotton fusarium wilt and Verticillium wilt, the main components of which are natural abscisic acid and Bacillus subtilis ZK-3, which can effectively It can prevent the occurrence of cotton wilt and verticillium wilt, and also has a certain control effect on fungal diseases such as cotton seedling blight and anthracnose, and can make cotton plants grow robustly, and improve cotton yield and quality.

Description

A seed coating agent against cotton fusarium wilt and verticillium wilt

1. Field

The invention belongs to a kind of agricultural pesticide, in particular to a seed coating agent made of natural abscisic acid and Bacillus subtilis ZK-3 (Bacillus Subtilis ZK-3).

2. Background technology

Natural abscisic acid (S-ABA), common name of pesticide: S-abscisin. Molecular formula: C ₁₅ H ₂₀ O ₄ , chemical name: 5-(1'-hydroxy-2',6',6'-trimethyl-4'-oxo-2'-cyclohexene-1'- base)-3-methyl-2-cis-4-trans-pentadienoic acid, the chemical structure is as follows

In 1963, abscisic acid was first extracted from young cotton fruit by Addicott et al. For more than 30 years, scientists from various countries have conducted extensive and in-depth research on the physiological activity of plants. Studies have shown that abscisic acid is the "stress-resistance inducing factor" and "balanced growth factor" of plants, which can initiate the expression of plant stress-resistant genes, activate the plant's endogenous immune system, and induce plants to respond to biotic and abiotic stresses (drought, low temperature, Salt-alkali, diseases and insect pests) produce resistance, improve the drought resistance, cold resistance, disease resistance and saline-alkali resistance of plants, and can be widely used in agriculture, forestry, animal husbandry, landscaping, ecological vegetation restoration and other fields.

Bacillus subtilis ZK-3 (Bacillus Subtilis ZK-3), which was handed over to the General Microorganism Center for preservation on April 21, 2003, with the strain preservation number CGMCC No.0924) is a plant endophytic bacterium that can parasitize cotton plants In the vascular bundle, and produce antibacterial substances to prevent the growth of pathogenic bacteria of cotton fusarium wilt and Verticillium wilt.

Cotton Fusarium wilt and Verticillium wilt are one of the serious diseases of cotton that are very difficult to control, and cause huge economic losses to cotton production every year, especially the transgenic insect-resistant cotton, which has a high incidence. Because the pathogenic bacteria of Fusarium wilt and Verticillium wilt invade the vascular bundles of cotton plants, general medicaments cannot enter the vascular bundles to kill the pathogens. Therefore, there are currently no effective control methods and medicaments at home and abroad.

When the present inventor uses the seed coating agent of natural abscisic acid combination, finds that natural abscisic acid just starts stress resistance gene when cotton seed germinates, improves the resistance ability of cotton seedling to adversity, and cotton plant grows vigorously, disease resistance and cold resistance, Drought resistance is greatly enhanced; when the seed coating agent containing Bacillus subtilis ZK-3 is used, it is found that Bacillus subtilis ZK-3 can parasitize in the vascular bundle of cotton plants with the growth of cotton seedlings, helping cotton plants effectively resist Fusarium wilt, Verticillium wilt pathogen attack. Surprisingly, the present inventor added natural abscisic acid and Bacillus subtilis ZK-3 in the seed coating agent simultaneously, and adjusted respective optimum dosage, found that this seed coating agent can effectively prevent fusarium wilt, yellowing of cotton. The occurrence of wilt also has certain control effects on fungal diseases such as cotton seedling blight and anthracnose, and can make cotton plants grow robustly, improve cotton yield and quality, so the present invention has been completed.

3. Contents of the invention:

According to the above, the object of the present invention is to provide a new seed coating agent, which can effectively prevent the occurrence of Fusarium wilt and Verticillium wilt of cotton, and can make cotton plants grow robustly, and improve cotton yield and quality.

The seed coating agent that the present invention adopts, main component is natural abscisic acid (common name of pesticide: S-inducer) and bacillus subtilis ZK-3 (Bacillus Subtilis ZK-3), preservation of common microorganism center, bacterial strain preservation number CGMCC No. 0924).

Generally speaking, the natural abscisic acid used in the present invention is used at a concentration of 0.1-100.0ppm, preferably 0.1-50.0ppm, more preferably 1.0-10.0ppm;

Generally speaking, the concentration of Bacillus subtilis ZK-3 used in the present invention is: bacterial content 10 ¹ -10 ¹⁰ /mL, preferably: 10 ³ -10 ¹⁰ /mL, more preferably: 10 ⁵ -10 ⁹ cells/mL.

Generally speaking, in the seed coating agent of the present invention, the mixing weight ratio of Bacillus subtilis ZK-3 and natural abscisic acid is between 1:1-3000, preferably between 1:1-1000.

The seed coating agent of the present invention can be prepared in a conventional manner, using auxiliary agents commonly used in the seed coating agent processing technology. As described in the second edition of "Pesticide Formulation Processing Technology" edited by Liu Bulin (Chemical Industry Press, 1998) "Seed Coating Agent Processing", or "Pesticide Processing Technology" edited by Zhang Baomin (Central Plains Peasant Publishing House, 1996) ) "Seed coating agent processing".

1. Available binders and concentrations (% = percentage by weight):

Sodium carboxymethyl starch 0.1%--20%, preferably 1.0%--10%

Soluble starch 0.1%--25%, preferably 1.5%--10%

Sodium carboxymethyl cellulose 0.1%--20%, preferably 1.0%--5.0%

Hydroxypropyl methylcellulose 0.1%--20%, preferably 1.0%--10%

Hydroxypropyl cellulose 0.1%--30%, preferably 1.0%--8.0%

Sodium alginate 0.1%--20%, preferably 1.0%--10%

Polyvinyl alcohol 0.1%--20%, preferably 2.0%--6.0%

Polyethylene glycol 0.1%--30%, preferably 1.0%--8.0%

Gelatin 0.1%--20%, preferably 1.0%--10%

Pectin 0.1%--20%, preferably 1.0%--10%

Gum Arabic 0.1%--20%, preferably 1.0%--10%

2. Available fungicides and concentrations (% = percentage by weight)

Carbendazim, trifenazol, thiram, captan, tufunxiao, triamron, etc., the use concentration is 0.1%-30%, preferably 1%-15%, (using one or two kinds of compound) .

3. Available pesticides and concentrations (% = percentage by weight)

Carbofuran, phorate, Proton, Dimehypo, Diphorate, Pyrethrins, or Pyrethroids. The use concentration is 1%-40%, preferably 5%-25%. (Using one or two kinds of compound)

4. A particularly preferred seed coating composition is as follows (%=percentage by weight):

Carbendazim 50-120 grams, preferably 80-120 grams

Carbofuran 100-300 grams, preferably 150-240 grams

Sodium carboxymethyl cellulose solution 1L (containing 10 grams of dry matter)

Abscisic acid 1.0-3.0 mg preferably 1.0-1.5 mg

Bacillus subtilis ZK-3 liquid 1mL-5 mL (bacteria ^{content 107-109} /mL)

Delint cotton species 30.0-50.0 kg

Add carbendazim, carbofuran, abscisic acid, etc., which have been dissolved in a small amount of water, and Bacillus subtilis ZK-3 solution into a solution containing 10 grams of sodium carboxymethylcellulose, add water to a volume of 1 L, stir well, Spray evenly on the surface of 30-50 kg of delint cotton seeds with a sprayer, dry and store in the dark for at least 3 days, and then sow conventionally.

The present invention has the following advantages:

1. This seed coating agent can effectively prevent the occurrence of cotton wilt and verticillium wilt, and also has a certain control effect on fungal diseases such as cotton seedling blight and anthracnose, and can make cotton plants grow robustly and increase cotton production. and quality;

2. The preparation method of this kind of coating agent is simple;

3. Easy to use.

4. Specific implementation

Example 1:

Location: Wusu area, Xinjiang

Seed coating composition is as follows (%=percentage by weight):

Carbendazim 100g

Carbofuran 240g

Sodium carboxymethyl cellulose solution 1L (containing 10 grams of dry matter)

Abscisic Acid 1.0 mg

Bacillus subtilis ZK-3 liquid 4mL (bacteria content ¹⁰⁹ /mL)

Add carbendazim, carbofuran, abscisic acid, etc., which have been dissolved in a small amount of water, and Bacillus subtilis ZK-3 solution into a solution containing 10 grams of sodium carboxymethylcellulose, add water to a volume of 1 L, stir well, Spray evenly on the surface of 40 kg of delint cotton seeds with a sprayer, dry and place in the dark for at least 3 days, and then sow conventionally.

Cotton plant growth status survey form

Cotton Seed Status Germination rate blight infection rate Incidence rate of Fusarium wilt and Verticillium wilt Seed cotton yield (kg/mu) Seed coating agent of the present invention 95% 3% 5% 110.0 Commercially available common seed coating agent (63% pyridyl wilt dry powder, product of Beinong (Haili) Zhuozhou Seed Coating Agent Co., Ltd.) 84% 7% twenty three% 95.5 Cotton seed without seed coating 70% 30% 35% 49.3

The test results show that the use of the seed coating agent of the present invention improves the emergence rate of cotton seeds, greatly reduces the incidence of Fusarium wilt, Verticillium wilt and blight, and greatly increases the yield.

Example 2:

Location: Santai area, Sichuan

Seed coating composition is as follows (%=percentage by weight):

Carbendazim 80g

Carbofuran 170g

Sodium carboxymethylcellulose solution 1L (containing 8 grams of dry matter)

Abscisic Acid 1.0 mg

Bacillus subtilis ZK-3 liquid 2mL (bacteria content ¹⁰⁹ /mL)

Add carbendazim, carbofuran, abscisic acid, etc., which have been dissolved in a small amount of water, and Bacillus subtilis ZK-3 solution into a solution containing 8 grams of sodium carboxymethylcellulose, add water to a volume of 1 L, stir well, Spray evenly on the surface of 50 kg of delint cotton seeds with a sprayer, dry and place in the dark for at least 3 days, and then sow conventionally.

Cotton plant growth status survey form

Cotton Seed Status Germination rate Anthrax Infection Rate Incidence rate of Fusarium wilt and Verticillium wilt Seed cotton yield (kg/mu) Seed coating agent of the present invention 98% 1% 3% 104.2 Commercially available common seed coating agent (20% Folger suspension, Hebei Fangzhou Agricultural Technology Co., Ltd.) 94% 10% 19% 89.5 Cotton seed without seed coating 86% 26% 31% 52.9

The test results show that the use of the seed coating agent of the invention improves the emergence rate of cotton seeds, greatly reduces the incidence of fusarium wilt, verticillium wilt and anthracnose, and greatly increases the yield.

Example 3:

Location: Jianli District, Hubei

Seed coating composition is as follows (%=percentage by weight):

Rustling 30g

Baomianfeng 200g

Soluble starch solution 1L (containing 12 grams of dry matter)

Abscisic Acid 1.0 mg

Bacillus subtilis ZK-3 liquid 3mL (bacteria content ¹⁰⁷ /mL)

Add the fenoxin, Baomianfeng, abscisic acid, etc. that have been dissolved in a small amount of water and Bacillus subtilis ZK-3 into the solution containing 12 grams of soluble starch, add water to a volume of 1 L, stir well, and spray evenly on the surface with a sprayer 50 kg of delint cotton seed surface, after drying, place it in the dark for at least 3 days, and then sow conventionally.

Cotton plant growth status survey form

Cotton Seed Status Germination rate Anthrax Infection Rate Incidence rate of Fusarium wilt and Verticillium wilt blight infection rate Seed cotton yield (kg/mu) Seed coating agent of the present invention 97% 5% 5% 6% 98.4 Commercially available common seed coating agent (63% pyridyl wilt dry powder, product of Beinong (Haili) Zhuozhou Seed Coating Agent Co., Ltd.) 94% 8% 18% 15% 85.1 Cotton seed without seed coating 80% 20% 31% 36% 44.9

The test results show that the use of the seed coating agent of the present invention improves the emergence rate of cotton seeds, greatly reduces the incidence of Fusarium wilt, Verticillium wilt, blight and anthracnose, and greatly increases the yield.

Claims (10)

1, a kind of resisting cotton blight kind clothing agent of disease and verticillium wilt of withering, it is characterized in that: main component is that natural abscisic acid and culture presevation number are the bacillus subtilis ZK-3 of CGMCC No.0924 (Bacillus Subtilis ZK-3), the natural abscisic acid working concentration is 0.1-100.0ppm, and bacillus subtilis ZK-3 working concentration is a bacterial content 10 ¹-10 ¹⁰Individual/mL, bacillus subtilis ZK-3 compares between 1 with the mixed weight of natural abscisic acid: 1-3000.

2, the resisting cotton blight according to claim a kind clothing agent of disease and verticillium wilt of withering is characterized in that: also comprise binding agent, bactericide or insecticide; Binding agent and weight percent concentration: sodium carboxymethyl starch 0.1%--20% or soluble starch 0.1%--25% or sodium carboxymethylcellulose 0.1%--20% or hydroxypropyl methylcellulose 0.1%--20% or hydroxypropyl cellulose 0.1%--30% or sodium alginate 0.1%--20% or polyvinyl alcohol 0.1%--20% or polyethylene glycol 0.1%--30% or gelatin 0.1%--20% or pectin 0.1%--20% or gum Arabic 0.1%--20%; Bactericide and weight percent concentration are: in the rust spirit of carbendazim or powder or tmtd or captan or hydroxyisoxazole or the dazomet a kind or 2 kinds, and working concentration is at 0.1%--30%; Insecticide and weight percent concentration are: in carbofuran or thimet or thimet sulfonoxide or dimehypo or disulfoton or the pyrethrins a kind or 2 kinds, working concentration is at 1%--40%.

3, the resisting cotton blight according to claim a kind clothing agent of disease and verticillium wilt of withering, it is characterized in that: the natural abscisic acid working concentration is 0.1-50.0ppm, bacillus subtilis ZK-3 working concentration is 10 ³-10 ¹⁰Individual/mL.

4, the resisting cotton blight according to claim a kind clothing agent of disease and verticillium wilt of withering, it is characterized in that: the natural abscisic acid working concentration is 1.0-10.0ppm, bacillus subtilis ZK-3 working concentration is 10 ⁵-10 ⁹Individual/mL.

5, the resisting cotton blight according to claim a kind clothing agent of disease and verticillium wilt of withering is characterized in that: the mixed weight of bacillus subtilis ZK-3 and natural abscisic acid is than between 1: between the 1-1000.

6, the resisting cotton blight according to claim 2 kind clothing agent of disease and verticillium wilt of withering is characterized in that: the binding agent of employing and weight percent concentration: sodium carboxymethyl starch 1.0%--10% or soluble starch 1.5%--10% or sodium carboxymethylcellulose 1.0%--5.0% or hydroxypropyl methylcellulose 1.0%--10% or hydroxypropyl cellulose 1.0%--8.0% or sodium alginate 1.0%--10% or polyvinyl alcohol 2.0%--6.0% or polyethylene glycol 1.0%--8.0% or gelatin 1.0%--10% or pectin 1.0%--10% or gum Arabic 1.0%--10%.

7, the resisting cotton blight according to claim 2 kind clothing agent of disease and verticillium wilt of withering, it is characterized in that: the bactericide of employing and weight percent concentration are: in the rust spirit of carbendazim or powder or tmtd or captan or hydroxyisoxazole or the dazomet a kind or 2 kinds, working concentration is at 1%-15%.

8, the resisting cotton blight according to claim 2 kind clothing agent of disease and verticillium wilt of withering, it is characterized in that: the insecticide of employing and weight percent concentration are: in carbofuran or thimet or thimet sulfonoxide or dimehypo or disulfoton or the pyrethrins a kind or 2 kinds, working concentration is at 5%-25%.

9, the resisting cotton blight according to claim 2 kind clothing agent of disease and verticillium wilt of withering, composed as follows:

Carbendazim 50-120 gram

Carbofuran 100-300 gram

Carboxymethylcellulose sodium solution 1L

Abscisic acid 1.0-3.0 milligram

Bacillus subtilis ZK-3 liquid 1mL-5mL

Ginning cotton seed 30.0-50.0 kilogram

Wherein, contain 10 gram dry matteies in the described carboxymethylcellulose sodium solution, bacterial content is 10 in the bacillus subtilis ZK-3 liquid ⁷-10 ⁹Individual/mL.

10, the resisting cotton blight according to claim 2 kind clothing agent of disease and verticillium wilt of withering, composed as follows:

Carbendazim 80-120 gram

Carbofuran 150-240 gram

Carboxymethylcellulose sodium solution 1L

Abscisic acid 1.0-1.5 milligram

Bacillus subtilis ZK-3 1mL-5mL

Ginning cotton seed 30.0-50.0 kilogram

Wherein, contain 10 gram dry matteies in the described carboxymethylcellulose sodium solution, bacterial content is 10 in the bacillus subtilis ZK-3 liquid ⁷-10 ⁹Individual/mL.