CN100410652C - A teaching kit for biological evolution at the cellular level - Google Patents

Abstract

The invention relates to a teaching kit for biological evolution at the cell level. Aiming at the biological evolution experiment at the cell level, reagents and materials are optimally configured according to 10 groups of experimental teaching standard classes, including scalpels, toothpicks, coverslips, and tweezers. , glass slides, small tubes of strains (preserved in glycerol tubes after E.coli puncture culture), 0.02% Jenner's green staining solution, 6cm small petri dish, 10ml distilled water drop bottle, medium (dry powder), 50ml distilled water, etc. Each kit is equipped with a user manual, which can be used by a standard class to carry out experiments. The invention is applied to the teaching of the introduction to biological science experiments, which can save teachers' experiment preparation time, reduce the configuration of experiment personnel, reduce the technical difficulty of experiment preparation, and enable the biological evolution experiment at the cell level to be completed efficiently and quickly and achieve good experimental results .

Description

A teaching kit for biological evolution at the cellular level

technical field

The invention relates to a teaching kit for biological evolution at the cell level, which is used for the biological evolution experiment at the cell level in the teaching of the introduction to biological science experiments.

Background technique

At present, my country's biological science experiment teaching is facing the dilemma of high investment and low production efficiency. General laboratories need to be equipped with experimental teachers and experimenters at the same time. A lot of preparatory work is required before the experiment. It is often necessary to purchase a variety of reagents and materials related to the experiment, and then prepare them in groups according to the requirements of the experiment and the number of people in the experiment. The completion of these tasks requires a certain number of experimental personnel, and the reagents and materials purchased at the same time are often larger than the actual needs, so there are wastes to varying degrees, which increases the cost of the experiment. At the same time, the technical difficulty of the experimental preparation is also very high. The amount used by each experimental group, the precise measurement and distribution of the amount of each reagent and each material must be meticulous and accurate, so as to ensure the normal progress of the experiment. It can be seen that preparing for experiments needs to consume and waste a lot of manpower, material resources, and financial resources, and a new way of preparing for experiments is urgently needed. Although domestic and imported scientific research kits have been widely used in my country's biological science research, with the characteristics of convenience, speed, efficiency, and accurate and reliable results, there is no patented kit product suitable for the teaching of introduction to biological science experiments in China. . At the same time, because scientific research kits are generally aimed at a specific experimental link and have strong technical characteristics, their content is difficult to adapt to teaching experiments on the surface. Therefore, it is particularly necessary and urgent to invent a teaching kit suitable for biological science experiment teaching.

Contents of the invention

The purpose of the present invention is to provide a teaching kit suitable for biological evolution at the cell level in view of the deficiencies of the prior art and actual needs, which can be conveniently and directly used to complete teaching experiments, ensure high efficiency of experiments, and save experiment costs.

In order to achieve such a purpose, the present invention aims at the biological evolution experiment at the cell level, and packs the reagents and materials in the kit, and optimizes the configuration according to 10 groups of one experimental teaching standard class. The materials in the kit are designed according to the amount used by 10 experimental groups, including strain tubes (E coli, stored in glycerol tubes after E. coli puncture culture), tweezers, scalpels, 0.02% Jenner’s green dye solution, distilled water droppers , small petri dish, glass slide, cover glass, toothpick, LB medium (Luria-Bertani Medium dry powder, containing agar powder), distilled water, etc. Each kit is equipped with a user manual, which can be used by a standard class to carry out experiments.

The teaching kit of the present invention contains a complete set of materials and reagents for biological evolution experiments at the cell level. Materials are placed in the kit according to the amount used by 10 experimental groups, and each group includes a scalpel, a bag of toothpicks, a pack of coverslips, a pair of tweezers, a pack of slides, a bottle of Jenner's green stain, A small Petri dish, a dropper bottle of distilled water, etc. The dosage of each group of reagents and materials is the optimized value obtained through many experiments and teachings over the years. The weight percentage concentration of Jenner's green dye solution is 0.02%, the distilled water drop bottle is 10ml, the petri dish is 6cm, and the slide glass is 3 slices, 10 coverslips, 2 toothpicks. In the kit, there is also a small tube of strains, Escherichia coli strains preserved in glycerol, a bottle of LB medium (dry powder, 0.625g), a bottle of 50ml distilled water and a kit manual.

One group of materials in the kit of the present invention can be used by a group of students (2-3 people), and ten groups of materials can be used by a standard class (30 people) to carry out experiments. It can also be equipped with corresponding experimental guidance materials and CDs according to different needs.

Before the experiment, the experiment teacher or preparer only needs to do simple preparatory work: the experiment teacher adds 50ml of distilled water to the medium dry powder to prepare LB medium, and sterilizes it with a 6cm small petri dish and a toothpick at high temperature before starting the class. Pour 10 plates on the ultra-clean workbench; streak and activate Escherichia coli on the small plate 24 hours in advance; the activated E.coli can be stored in a 4°C refrigerator and distributed to each group before the experiment. Fresh commelina can be collected on the day of the experiment. The experimental items were distributed to each group before the experiment started. During the experiment, first carry out the staining observation of E coli bacterium Jenner's green: drop a drop of 0.02% Jenner's green staining solution in the center of the slide, use an inoculation needle to pick up freshly grown E. Spread it out to make it evenly distributed, stain it for 3 minutes, add a cover glass, and observe it under a microscope. Bacterial cells are stained green, rod-shaped, and the movement of living bacteria can be observed under a microscope. Observation of mitochondrial Jenner's green staining of human oral mucosa cells: Take 0.02% Jenner's green staining solution and place it in the center of a clean glass slide, scrape the inner wall of the oral cavity with a sterilized toothpick, and use a little heavier force to obtain cells with more vigorous vitality. Place the scrapings in the staining solution on the glass slide, mix well, cover with a cover glass after 2 to 3 minutes, and observe under a microscope. Observation with low magnification first and then high magnification. Under high magnification, it can be seen that some rod-shaped and round particles dyed bright green are scattered in the cytoplasm of oral mucosal cells, which are mitochondria. Observe the morphology and staining of mitochondria in human oral mucosa cells, and compare their similarity with Escherichia coli in morphology and staining. Observation of Commelina Epidermal Cells: First take a clean glass slide and add a drop of water on it. Take fresh Commelina leaves, use a blade to cross-cut a gap on the back of the blade, insert tweezers from the gap parallel to the surface, and tear off the epidermis. After the epidermis is torn off, put it face down into the water drop of the glass slide, and cover it with a cover glass , Use absorbent paper to absorb excess water, and then observe the shape, size, chloroplast, vacuole and other structures of plant cells under the microscope.

The teaching kit of the present invention provides a tube of Escherichia coli strain preserved in glycerin, and the experiment teacher only needs to activate it and distribute the articles to each group to carry out the experiment. On the one hand, this reduces the allocation of experimental personnel, on the other hand, it also makes the experimental teaching fast and efficient, and students can obtain relatively standard and reliable experimental results.

The present invention provides an economical and practical teaching kit for the teaching of the introduction to biological science experiments. Experimental reagents and materials are optimally configured according to 10 groups of experimental teaching standard classes, which avoids unnecessary waste and saves reagents and materials. During the experiment, after the teacher reads the manual of the kit, he only needs to distribute the materials and reagents required by each group to each group according to the instructions, and then the experimental teaching can be carried out, which saves the tedious preparation of the experiment and saves the time and energy of the teacher . At the same time, the improvement of work efficiency can reduce the configuration of experimenters to a certain extent. According to the teacher's classroom explanation and the manual attached to the kit, students can have a good grasp of the experimental content and successfully complete the experiment in a relatively short period of time. The kit of the invention enables the biological evolution experiment at the cell level to be completed efficiently and quickly and achieves good experimental results, and can minimize the financial, material and manpower required for the preparation of the experiment.

Description of drawings

Figure 1 is a display of the reagents and medicines contained in the kit of the present invention.

In the figure, 1 is a kit, 2 is a scalpel, 3 is a toothpick, 4 is a cover glass, 5 is tweezers, 6 is a glass slide, 7 is a small tube of bacteria (preserved in a glycerol tube after E coli puncture culture), 8 0.02% Jenner's green dye solution by weight percent concentration, 9 is a 6cm petri dish, 10 is a 10ml distilled water drop bottle, 11 is LB medium (dry powder), 12 is 50ml distilled water, and 13 is a kit manual.

Detailed ways

The technical solution of the present invention will be further described below in conjunction with the accompanying drawings.

Fig. 1 is an embodiment of the teaching kit provided by the present invention, which contains a complete set of materials and reagents required for the experiment of biological evolution at the cellular level. As shown in Figure 1, the experimental reagents and materials are optimally configured according to 10 groups of one experimental teaching standard class. The kit 1 includes the materials required by 10 groups, and each group includes a scalpel 2, a bag of toothpicks 3, a Pack of 10 coverslips 4, a pair of tweezers 5, a pack of 3 slides 6, a bottle of Jenner's Green staining solution with a concentration of 0.02% by weight 8, a 6cm petri dish 9, a bottle of 10ml distilled water Dropping bottle 10, the common materials in the kit include a strain tube 7, Escherichia coli strains stored in glycerin, a bottle of 0.625g LB medium 11 (dry powder), a bottle of 50ml distilled water 12 and a kit for use Manual 13.

The amount of materials in each of the above-mentioned small packages in the kit is the optimized value obtained from experimental teaching over the years. One set of materials can be used by a group of students (2-3 people), and ten sets of materials can be used by a standard class (30 people) to carry out experiments. .

During the experiment, take out each group of materials in the kit and assign them to each experimental group. According to the instructions in the manual, the teacher can guide the students to complete the experiment by explaining. In addition, the kit can also be equipped with corresponding supporting experimental guidance materials and CDs according to different needs, and the experiment can be carried out in combination with teaching, and the effect is better.

Claims (2)

1. the teaching kit of the biological evolution of a cellular level, it is characterized in that the required reagent and the material of biological evolution experiment of cellular level is optimized configuration by 10 groups one experimental teaching standard class, every group comprises that a scalper (2), one bag of toothpick (3), bag 10 cover glasses (4), one wrap 3 microslides (6), one bottle of green dye liquor of Zhan Na Shi (8), 6 cm double dish (9), one bottle of 10ml distilled water drop bottle (10) to tweezers (5),, and wherein the green dye liquor weight percent concentration of Zhan Na Shi is 0.02%; Be equipped with a bacterial classification tubule (7), one bottle of 0.625g LB culture medium dry powder (11), one bottle of 50ml distilled water (12) and a kit service manual (13) in the kit in addition, wherein the coli strain of the built-in glycerine preservation of bacterial classification tubule (7).

2. according to the teaching kit of the biological evolution of the cellular level of claim 1, it is characterized in that also being equipped with experiment instruction teaching material and CD in the kit (1).

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