CN100402045C - Starfish and seaweed compound product and its preparing method - Google Patents
Starfish and seaweed compound product and its preparing method Download PDFInfo
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- CN100402045C CN100402045C CNB2004100509126A CN200410050912A CN100402045C CN 100402045 C CN100402045 C CN 100402045C CN B2004100509126 A CNB2004100509126 A CN B2004100509126A CN 200410050912 A CN200410050912 A CN 200410050912A CN 100402045 C CN100402045 C CN 100402045C
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- asterias amurensis
- amurensis lutken
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- Medicines Containing Plant Substances (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The present invention relates to a composite product of starfish and seaweed, which is used as a medicament or a health care product for treating cardio-cerebrovascular disease, and a preparation method thereof. The composite product is a composite extract prepared from starfish and sea tangle as raw materials by a specific process, the effective ingredient of the composite product can effectively lower the content of cholesterol, triglyceride and low-density lipoprotein in blood and increase the content of high-density lipoprotein. The method for preparing the composite product of starfish and seaweed comprises the following steps: starfish and seaweed are respectively leached by an intercrossing process of water extraction and ethanol extraction at different temperatures, and then, extracts are mixed. The composite product of starfish and seaweed, which is prepared by the preparation method, is a natural product, is safe in use and has obvious treating and preventing effects on bearing premature, hyperlipemia, apoplexy, hypertension, cerebral arteriosclerosis, coronary heart disease, etc.
Description
Technical field
The present invention relates to a kind of medicine or the Asterias amurensis Lutken of health product and Sargassum composite article and preparation method thereof as cardiovascular and cerebrovascular disease.
Background technology
Asterias amurensis Lutken has another name called starfish, belongs to the invertebrates Echinodermata, and Asteroidea mainly is distributed on the bed-sit ground, shallow sea or reef all over the world, and profile is generally 5 wrists, also has 4,6, nearly 50 wrists that have.Research is indicated, the Asterias amurensis Lutken body contains chemical substances such as Saponin, sterol, polysaccharide, insulin type, carotenoid, vitamins, protein, polypeptide, aminoacid, has multiple pharmacologically actives such as anticancer, antibiotic, antiviral, antiinflammatory, antiulcer, blood pressure lowering.The economic worth of Asterias amurensis Lutken is little, can only dry and make farming fertilizer; In recent years at raise immunity, kidney invigorating and YANG supporting, defying age, preparation plasma substitute, do aspect feedstuff or the bait some researchs are arranged also, but widespread usage not as yet.
Sargassum is a kind of plant that lives in fully in the water, and stock number is huge.Sargassum also contains nutrient substance such as the vitamin abundanter than general terrestrial plant, trace element, mineral except that containing protein that general biology has, carbohydrate, fat.Also have phycobilin that many general plants do not have, Brown algae flavin, mannitol, iodine, nicotinic acid, agar, carrageenan or the like in addition.The used Sargassum of the present invention is one or more in Thallus Laminariae (Thallus Eckloniae), narrow leaf Thallus Laminariae (Thallus Eckloniae), plan Thallus Laminariae (Thallus Eckloniae), Thallus Laminariae, Alga Sgrgassi Enerves, Sargassum fusiforme (Harv.) Setch, the Sargassum phyllocystum Tseng et Lu,Sargassum horneri (Turn.) C. Ag. (Fucus horneri (Turn.)C.Ag.,Spongocarpus horneri Kutz.), these algae can be used as food or are used for fields such as food industry, rubber industry, medical industry, chemical industry: refine medicine, glue, manufacturing methane and make feedstuff and fertilizer etc., have using value.
Summary of the invention:
The object of the invention is to develop a kind of medicine or the Asterias amurensis Lutken of health product and Sargassum composite article and preparation method thereof as cardiovascular and cerebrovascular disease.
In to Asterias amurensis Lutken and the work of Sargassum activity research, the composite extract that our discovery obtains by specific technology from above-mentioned raw materials, can effectively reduce cholesterol, triglyceride and low density lipoprotein, LDL content in the blood, high density lipoprotein increasing content, cardiovascular and cerebrovascular diseases such as premature beat, hyperlipidemia, apoplexy, hypertension, cerebral arteriosclerosis, coronary heart disease there are significant treatment and preventive effect, and are natural product, safety.
The present invention is a raw material with Asterias amurensis Lutken and Sargassum, by raw water carry, alcohol extraction, merceration, hot dipping, concentrate, technical process such as allotment, obtain having the goods that cardiovascular and cerebrovascular diseases such as premature beat, hyperlipidemia, apoplexy, hypertension, cerebral arteriosclerosis, coronary heart disease had significant treatment and preventive effect, these goods can be made into medicine or health product, thereby have realized purpose of the present invention.
Essence of the present invention is that the natural constituent medicinal or the health product purposes that has treatment and prevention cardiovascular and cerebrovascular disease in Asterias amurensis Lutken and the Sargassum raw material is extracted, this leaching process and described preparation method, be the technology that the water that utilizes different temperatures is carried, alcohol extraction intersects, respectively Asterias amurensis Lutken and Sargassum are carried out lixiviate, then extract is mixed by a certain percentage and make, concrete grammar may further comprise the steps:
(1) lixiviate of Asterias amurensis Lutken raw material, method have following three kinds:
(a) with Asterias amurensis Lutken raw material alcoholic strength be the sufficiently long time of ethanol merceration of 20～70 degree, filter, get its filtrate and remove alcohol, concentrate, get extract A 1;
(b) be that 20～70 ethanol heat of spending are carried the sufficiently long time, extracted 30～70 ℃ of temperature with Asterias amurensis Lutken raw material alcoholic strength, filter, get its filtrate and remove alcohol, concentrate, get extract A 2;
(c) the Asterias amurensis Lutken raw material is carried the sufficiently long time, extracted 50～99 ℃ of temperature with hydro-thermal, filter, get its filtrate concentrating, get extract A 3;
(2) lixiviate of Sargassum raw material, method have following three kinds:
(a) with Sargassum raw material alcoholic strength be the sufficiently long time of ethanol merceration of 20～70 degree, filter, get its filtrate and remove alcohol, concentrate, get extract B 1;
(b) be that 20～70 ethanol heat of spending are carried the sufficiently long time, extracted 30～70 ℃ of temperature with Sargassum raw material alcoholic strength, filter, get its filtrate and remove alcohol, concentrate, get extract B 2;
(c) the Sargassum raw material is carried the sufficiently long time, is extracted 50～99 ℃ of temperature with hydro-thermal, filters, and gets its filtrate concentrating, and gets extract B 3;
(3) with extract obtained in above-mentioned (1) and (2) by extract A 1 and extract B 1, or extract A 1 and extract B 2, or extract A 1 and extract B 3, or extract A 2 and extract B 1, or extract A 2 and extract B 2, or extract A 2 and extract B 3, or extract A 3 and extract B 1, or extract A 3 and extract B 2, or extract A 3 and extract B 3, admittedly containing the amount ratio by solubility in the solution is 1: 0.2～8 mixed, boil 10～50min then, cooling, filter, final gained solution concentration is drying to obtain the final extract of this Asterias amurensis Lutken and Sargassum composite article.
Said Asterias amurensis Lutken and Sargassum composite article can be made into tablet, capsule, granule or oral liquid.The final extract that is about to appeal gained adds suitable additives and makes tablet, capsule, granule, oral liquid, or final extract directly is made into oral liquid, takes 0.3～4g every day.
At the raw material of Asterias amurensis Lutken described in the step 1. is Echinodermata Asteroidea biology, as Craspidaster hesperus (mulleret Troschel). Craspidaster hesperus, knight Zhang Haixing Stellaster equestris, true five jiaos of Asterias amurensis Lutken Anthenea pentagonula, Flos Rosae Multiflorae Asterias amurensis Lutken Rosastersymbolicus, style of calligraphy characterized by hollow strokes maple Asterias amurensis Lutken Archaster typicus, Luo Shi Asterias amurensis Lutken Asterias rollestoni etc. are generally the solarization dry product.
At the raw material of Sargassum described in the step 2. is the ocean Brown algae, as Thallus Laminariae (Thallus Eckloniae) Laminariajaponica Areschoug, narrow leaf Thallus Laminariae (Thallus Eckloniae) Laminaria angustata Kjellman, intend Thallus Laminariae (Thallus Eckloniae) Eckloniopsis radicosa (Kjellman) Okamura, Thallus Laminariae Undaria Pinnatifida (Aarvey) Suringar, Alga Sgrgassi Enerves Sargassum (Turner) C.Agardh, Sargassum fusiforme (Harv.) Setch Sargassum fusiforme (Harvey) Setch, Sargassum phyllocystum Tseng et Lu,Sargassum horneri (Turn.) C. Ag. (Fucus horneri (Turn.)C.Ag.,Spongocarpus horneri Kutz.) Sargassum hornei (Turner) C.Agardh etc. are generally the solarization dry product.
In step 1., the Asterias amurensis Lutken raw material can be cleaned, dry, carry out lixiviate again after pulverizing, to improve the leaching rate of Asterias amurensis Lutken effective ingredient.
In step 1., the Sargassum raw material can be cleaned, dry, carry out lixiviate again after pulverizing, to improve the leaching rate of Sargassum effective ingredient.
The present invention adopts modern science and technology, and the pharmaceutical components in Asterias amurensis Lutken and the Sargassum raw material is extracted, and prepared Asterias amurensis Lutken and Sargassum composite article are natural product; safety, the reliable easily row of its preparation method is fit to large-scale production; free from environmental pollution, cost is low.
This Asterias amurensis Lutken and Sargassum composite article have cardiovascular and cerebrovascular diseases such as premature beat, hyperlipidemia, apoplexy, hypertension, cerebral arteriosclerosis, coronary heart disease are had significant treatment and preventive effect, and be natural product, safety can be used as the medicine or the health product for the treatment of cardiovascular and cerebrovascular disease.Take every day in right amount, can reach blood fat reducing, the effect of rehabilitation also has the effect of prevention and treatment simultaneously after effectively preventing the generation of apoplexy and promoting apoplexy to premature beat, hypertension, cerebral arteriosclerosis, coronary heart disease etc.
Significant effect: by following description of test:
Extracting solution I, the II experimentation to the rat blood pressure influence is given birth in the sea
1, material
1.1 animal SD is a rat, male and female are double gets, and body weight 180-220g is bought by Department of Health of Guangdong Province medical animal field.
1.2 extracting solution I, II are given birth in medicine sea, adopt distinct methods to extract the extract concentrated solution that makes for Asterias amurensis Lutken and Thallus Laminariae (Thallus Eckloniae), are provided by the Bai Xueping preparation.
1.3 instrument LMS-2B type two road physiology monitors (Chengdu Instruement Factory's product), CYS type medical pressure transducer (Ministry of Astronautics Industry's 627 institutes produce)
2, experimental technique
2.1 the adjusting of two road physiology monitors, is calibrated zero point by the instrument description of blood pressure determination and the pressure of conducting system is transferred to 12kp (80mmHg).
2.2 the connection of conducting system is full of paraffin with pressure transducer earlier, by three-way cock arterial cannulation is linked to each other with pressure transducer then, the syringe of another outlet of three-way cock and the 10ml that fills with heparin-saline links to each other.Make whole blood pressure conducting system be full of the heparin-saline of 5mg/ml by syringe, and the bubble in the venting conducting system.
2.3 operation and 20 of aortic cannulation rats are divided into the sea at random and give birth to 10 of extracting solution I groups, 10 of extracting solution II groups are given birth in the sea, lumbar injection urethane 1.2g/kg, dorsal position is fixed on the Mus dissecting table, cuts off the hair of cervical region, with the clean scalpel of wipes of alcohol, cut off skin from the neck median line, isolate trachea, under trachea, wear two lines, on trachea, cut a V-type mouth, insert tracheal intubation (plastic tube), a line fixed air cannula, another toe-in are pricked the other end of v-notch in case wound bleeding.From breast clavicle papillary muscles inner edge separately, expose common carotid artery and vagus nerve, isolate one section tremulous pulse, and under it, wear two lines, and a root knot is pricked distal end, and another root is standby, be clipped in proximal part with bulldog clamp, cut an osculum with eye scissors on common carotid artery, the aortic cannulation that is full of heparin-saline inserts common carotid artery, pricks with toe-in immediately and fixes it.Decontrol before the bulldog clamp, by three-way cock and the injection heparin-saline that is attached thereto system pressure is transferred to 12kp, blood enters pressure transducer when avoiding opening bulldog clamp.
2.4 administration is prepared this experiment and is adopted duodenal administration.Open the abdominal cavity from the abdomen median line, find duodenum, the tip thin plastic pipe that will be furnished with the 2ml syringe directly inserts duodenum and fixes it, closes the abdominal cavity then.After all operations finished, the heparin-saline that injects 0.1-0.2ml (1mg/ml) in sublingual vein made heparinization in the rat body.
2.5 decontroling bulldog clamp, blood pressure determination starts physiograph, chart speed is 10mm/s, recording blood pressure ripple and 5 are more than the waveform, inject the sea from duodenal intubation respectively and give birth to extracting solution I and Hai Sheng extracting solution II 0.5g/kg, behind administration 10,30,60,90,120,240min, starting---record, all the other time chart speeds are mean pressure (MAP), systolic pressure (SP), the diastolic pressure (DP) before and after the administration of 1mm/s continuous record.
3, result
The results are shown in Table 1.
Extracting solution I, II are given birth to the influence of rat blood pressure (x ± s) in table 1 sea
Annotate:
*: the administration front and back are to comparing P＜0.001 on the same group;
△ △: same time ratio is than P＜0.001 between two groups
Conclusion
1. give the sea through duodenum and give birth to extracting solution I, the 10min rat blood pressure begins to descend slightly after the administration, and the 60min blood pressure drops to minimum, but compares before and after the administration, does not have significant difference.Extracting solution I is given birth to not significantly effect of rat blood pressure in the prompting sea.
2. give the sea through duodenum and give birth to extracting solution II, the 10min rat blood pressure begins to descend after the administration, and is still very obvious to the 240min hypotensive effect, compares before and after the administration to have significant difference (P＜0.001).After this blood pressure slowly gos up.The effect that extracting solution II has significant reduction rat blood pressure is given birth in the prompting sea.
The specific embodiment
Embodiment 1: get Craspidaster hesperus (mulleret Troschel). 10kg, clean, dry, be ground into fritter, adding alcoholic strength is the edible ethanol 30kg of 60 degree, merceration is 5 days under room temperature, filters, and gets its filtrate and goes alcohol to be concentrated into 10kg, getting concentrated solution is extract A 1.Get Thallus Laminariae 10kg, clean, dry, be ground into fritter, adding alcoholic strength is the edible ethanol 100kg of 70 degree, merceration is 10 days under room temperature, filters, and gets its filtrate and goes alcohol to be concentrated into 15kg, getting concentrated solution is extract B 1.Get extract A 1, extract B 1 each 10kg mixing, boil 30min, cooled and filtered concentrates down in vacuum condition and dries to moisture≤3%, makes tablet, capsule, granule through conventional method again, or directly makes oral liquid.Eat 0.5～5g every day, and cardiovascular and cerebrovascular diseases such as premature beat, hyperlipidemia, apoplexy, hypertension, cerebral arteriosclerosis, coronary heart disease are had significant treatment and preventive effect.
Embodiment 2: get Luo Shi Asterias amurensis Lutken 10kg, clean, dry, be ground into fritter, adding alcoholic strength is the edible ethanol 50kg of 50 degree, stirs 180min at 60 ℃ of following constant temperature, filters, and gets its filtrate and goes alcohol to be concentrated into 10kg, getting concentrated solution is extract A 2.Get Alga Sgrgassi Enerves 10kg, clean, dry, be ground into fritter, adding alcoholic strength is the edible ethanol 200kg of 55 degree, stirs 180min at 60 ℃ of following constant temperature, boils 30min, cooled and filtered is got its filtrate and is gone alcohol to be concentrated into 50kg, getting concentrated solution is extract B 2.Get extract A 2 10kg, extract B 220kg and mix, filter, concentrate down in vacuum condition again and dry, make tablet, capsule, granule through conventional method again, or directly make oral liquid to moisture≤3%.Eat 0.5～5g every day, and cardiovascular and cerebrovascular diseases such as premature beat, hyperlipidemia, apoplexy, hypertension, cerebral arteriosclerosis, coronary heart disease are had significant treatment and preventive effect
Embodiment 3: get knight Zhang Haixing 10kg, clean, dry, be ground into fritter, add 100kg water, stir 120min at 90 ℃ of following constant temperature, filter, getting filtrate is extract A 3.Get Sargassum phyllocystum Tseng et Lu,Sargassum horneri (Turn.) C. Ag. (Fucus horneri (Turn.)C.Ag.,Spongocarpus horneri Kutz.) 10kg, clean, dry, be ground into fritter, add 200kg water, stir 120min at 90 ℃ of following constant temperature, filter, getting filtrate is extract B 3.Get extract A 3 50kg, extract B 3 20kg and mix, boil 30min, cooled and filtered concentrates down in vacuum condition and dries to moisture≤3%, makes tablet, capsule, granule through conventional method again, or directly makes oral liquid.Eat 0.5～5g every day, and cardiovascular and cerebrovascular diseases such as premature beat, hyperlipidemia, apoplexy, hypertension, cerebral arteriosclerosis, coronary heart disease are had significant treatment and preventive effect.
Embodiment 4: get knight Zhang Haixing 10kg, clean, dry, be ground into fritter, adding alcoholic strength is the edible ethanol 50kg of 30 degree, stirs 180min at 65 ℃ of following constant temperature, filters, and gets its filtrate and goes alcohol to be concentrated into 10kg, getting concentrated solution is extract A 2.Get Sargassum fusiforme (Harv.) Setch 10kg, clean, dry, be ground into fritter, add 200kg water, stir 180min at 70 ℃ of following constant temperature, boil 30min, cooled and filtered, getting filtrate is extract B 3.Get extract A 25kg, extract B 3 30kg and mix, filter, concentrate down in vacuum condition again and dry, make tablet, capsule, granule through conventional method again, or directly make oral liquid to moisture≤3%.Eat 0.5～5g every day, and cardiovascular and cerebrovascular diseases such as premature beat, hyperlipidemia, apoplexy, hypertension, cerebral arteriosclerosis, coronary heart disease are had significant treatment and preventive effect.
Embodiment 5: get true five jiaos of Asterias amurensis Lutken 10kg, clean, dry, be ground into fritter, adding alcoholic strength is the edible ethanol 50kg of 70 degree, merceration is 30 days under room temperature, filters, and gets its filtrate and goes alcohol to be concentrated into 10kg, getting concentrated solution is extract A 1.Get narrow leaf Thallus Laminariae (Thallus Eckloniae) 10kg, clean, dry, be ground into fritter, add 200kg water, stir 200min at 55 ℃ of following constant temperature, filter, getting filtrate is extract B 3.Get extract A 1 10kg, extract B 3 80kg and mix, boil 30min, cooled and filtered concentrates down in vacuum condition and dries to moisture≤3%, makes tablet, capsule, granule through conventional method again, or directly makes oral liquid.Eat 0.5～5g every day, and cardiovascular and cerebrovascular diseases such as premature beat, hyperlipidemia, apoplexy, hypertension, cerebral arteriosclerosis, coronary heart disease are had significant treatment and preventive effect.
Embodiment 6: get Flos Rosae Multiflorae Asterias amurensis Lutken 10kg, clean, dry, be ground into fritter, adding alcoholic strength is the edible ethanol 50kg of 60 degree, stirs 180min at 50 ℃ of following constant temperature, filters, and gets its filtrate and goes alcohol to be concentrated into 10kg, getting concentrated solution is extract A 2.Get narrow leaf Thallus Laminariae (Thallus Eckloniae) 10kg, clean, dry, be ground into fritter, adding alcoholic strength is the edible ethanol 100kg of 20 degree, merceration is 30 days under room temperature, filters, and gets its filtrate and goes alcohol to be concentrated into 15kg, getting concentrated solution is extract B 1.Get extract A 2 4kg, extract B 1 15kg and mix, boil 30min, cooled and filtered concentrates down in vacuum condition and dries to moisture≤3%, makes tablet, capsule, granule through conventional method again, or directly makes oral liquid.Eat 0.5～5g every day, and cardiovascular and cerebrovascular diseases such as premature beat, hyperlipidemia, apoplexy, hypertension, cerebral arteriosclerosis, coronary heart disease are had significant treatment and preventive effect.
Embodiment 7: get style of calligraphy characterized by hollow strokes maple Asterias amurensis Lutken 10kg, clean, dry, be ground into fritter, adding alcoholic strength is the edible ethanol 30kg of 40 degree, merceration is 10 days under room temperature, filters, and gets its filtrate and goes alcohol to be concentrated into 10kg, getting concentrated solution is extract A 1.Get Thallus Laminariae (Thallus Eckloniae) 10kg, clean, dry, be ground into fritter, adding alcoholic strength is the edible ethanol 200kg of 40 degree, stirs 180min at 60 ℃ of following constant temperature, filters, and gets its filtrate and goes alcohol to be concentrated into 50kg, getting concentrated solution is extract B 2.Get extract A 1 10kg, extract B 2 20kg and mix, boil 30min, cooled and filtered concentrates down in vacuum condition and dries to moisture≤3%, makes tablet, capsule, granule through conventional method again, or directly makes oral liquid.Eat 0.5～5g every day, and cardiovascular and cerebrovascular diseases such as premature beat, hyperlipidemia, apoplexy, hypertension, cerebral arteriosclerosis, coronary heart disease are had significant treatment and preventive effect.
Embodiment 8: get true five jiaos of Asterias amurensis Lutken 10kg, clean, dry, be ground into fritter, add 100kg water, stir 200min at 60 ℃ of following constant temperature, filter, getting filtrate is extract A 3.Get Thallus Laminariae 10kg, clean, dry, be ground into fritter, adding alcoholic strength is the edible ethanol 100kg of 50 degree, merceration is 30 days under room temperature, filters, and gets its filtrate and goes alcohol to be concentrated into 15kg, getting concentrated solution is extract B 1.Get extract A 360kg, extract B 1 15kg and mix, boil 30min, cooled and filtered concentrates down in vacuum condition and dries to moisture≤3%, makes tablet, capsule, granule through conventional method again, or directly makes oral liquid.Eat 0.5～5g every day, and cardiovascular and cerebrovascular diseases such as premature beat, hyperlipidemia, apoplexy, hypertension, cerebral arteriosclerosis, coronary heart disease are had significant treatment and preventive effect.
Embodiment 9: get knight Zhang Haixing 10kg, clean, dry, be ground into fritter, add 100kg water, stir 150min at 80 ℃ of following constant temperature, filter, getting filtrate is extract A 3.Get Sargassum fusiforme (Harv.) Setch 10kg, clean, dry, be ground into fritter, adding alcoholic strength is the edible ethanol 200kg of 20 degree, stirs 180min at 40 ℃ of following constant temperature, filters, and gets its filtrate and goes alcohol to be concentrated into 50kg, getting concentrated solution is extract B 2.Get extract A 3 30kg, extract B 2 50kg and mix, boil 30min, cooled and filtered concentrates down in vacuum condition and dries to moisture≤3%, makes tablet, capsule, granule through conventional method again, or directly makes oral liquid.Eat 0.5～5g every day, and cardiovascular and cerebrovascular diseases such as premature beat, hyperlipidemia, apoplexy, hypertension, cerebral arteriosclerosis, coronary heart disease are had significant treatment and preventive effect.
Claims (4)
1. Asterias amurensis Lutken and Sargassum composite article is characterized in that this composite article is is raw material with Asterias amurensis Lutken and Thallus Laminariae (Thallus Eckloniae), by specific method make to the medicable composite extract of cardiovascular and cerebrovascular disease, this goods method for making may further comprise the steps:
(1), the lixiviate of Asterias amurensis Lutken raw material:
With Asterias amurensis Lutken raw material alcoholic strength is the ethanol merceration of 20～70 degree, filters, and gets its filtrate and removes alcohol, concentrates, and gets extract A 1;
(2) lixiviate of Sargassum raw material:
With Sargassum raw material alcoholic strength is the ethanol merceration of 20～70 degree, filters, and gets its filtrate and removes alcohol, concentrates, and gets extract B 1;
(3) with the extract A 1 of gained in above-mentioned (1) and (2) and extract B 1, to contain the amount ratio admittedly by solubility in the solution be 1: 0.2～8 mixed, boil 10～50min then, cooling, filter, with final gained solution concentration drying, promptly get the final extract of Asterias amurensis Lutken and Sargassum composite article.
2. the Asterias amurensis Lutken of preparation described in the claim 1 and the method for Sargassum composite article is characterized in that this method utilizes ethanol, respectively Asterias amurensis Lutken and Sargassum are carried out lixiviate, then extract are mixed and make, and concrete method for making may further comprise the steps:
(1), the lixiviate of Asterias amurensis Lutken raw material:
With Asterias amurensis Lutken raw material alcoholic strength is the ethanol merceration of 20～70 degree, filters, and gets its filtrate and removes alcohol, concentrates, and gets extract A 1;
(2) lixiviate of Sargassum raw material:
With Sargassum raw material alcoholic strength is the ethanol merceration of 20～70 degree, filters, and gets its filtrate and removes alcohol, concentrates, and gets extract B 1;
(3) with the extract A 1 of gained in above-mentioned (1) and (2) and extract B 1, to contain the amount ratio admittedly by solubility in the solution be 1: 0.2～8 mixed, boil 10～50min then, cooling, filter, with final gained solution concentration drying, promptly get the final extract of Asterias amurensis Lutken and Sargassum composite article.
3. according to the method for Asterias amurensis Lutken described in the claim 2 and Sargassum composite article, it is characterized in that described Asterias amurensis Lutken raw material is an Echinodermata Asteroidea biology, is the solarization dry product of Craspidaster hesperus (mulleret Troschel)., knight Zhang Haixing, true five jiaos of Asterias amurensis Lutkenes, Flos Rosae Multiflorae Asterias amurensis Lutken, style of calligraphy characterized by hollow strokes maple Asterias amurensis Lutken or Luo Shi Asterias amurensis Lutken.
4. according to the method for Asterias amurensis Lutken described in the claim 2 and Sargassum composite article, it is characterized in that described Sargassum raw material is the ocean Brown algae, is the solarization dry product of Thallus Laminariae (Thallus Eckloniae), narrow leaf Thallus Laminariae (Thallus Eckloniae), plan Thallus Laminariae (Thallus Eckloniae) Thallus Laminariae, Alga Sgrgassi Enerves or Sargassum fusiforme (Harv.) Setch Sargassum phyllocystum Tseng et Lu,Sargassum horneri (Turn.) C. Ag. (Fucus horneri (Turn.)C.Ag.,Spongocarpus horneri Kutz.).
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