CN100389784C - Application of effective part and effective component of Achillea alpina to anti-anaphylactic reaction - Google Patents
Application of effective part and effective component of Achillea alpina to anti-anaphylactic reaction Download PDFInfo
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- CN100389784C CN100389784C CNB2004101042313A CN200410104231A CN100389784C CN 100389784 C CN100389784 C CN 100389784C CN B2004101042313 A CNB2004101042313 A CN B2004101042313A CN 200410104231 A CN200410104231 A CN 200410104231A CN 100389784 C CN100389784 C CN 100389784C
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Abstract
The invention relates to an application of effective parts and effective components of alpine yarrow herb in preparing anti-allergic medicine.
Description
Technical field
The present invention relates to the purposes of a kind of alpine yarrow herb effective parts and composition antianaphylaxis thereof.
Background technology
The dry herb of Herba Achilleae Artemisia rupestris L..Seven, tap August, dry.10-50 centimetre of this product total length.Root and rhizome are cylindrical, and khaki is to taupe, and length is with minority short hairs root, and section is light yellow.Stem single or several, in the bending of rhizome place, diameter 1.5-3 centimetre, the close tomentellate of sprout and spray top, the bottom of old branch or branch is smooth, and inapparent stria is arranged, the surface is an aubergine often, section white, hollow.Function with cure mainly: heat-clearing and toxic substances removing, invigorating the stomach and promoting digestion, expelling wind and activating blood circulation.Be used for flu, allergy, food stagnation, snakebite, furuncle.
Skin allergy often has a lot of reasons, and the medicine for the treatment of skin allergy at present is also a lot.Herba Achilleae is the Uygur medicine medicinal herbs most in use, and bibliographical information Herba Achilleae herb contains chemical compounds such as flavonoid, keto acid class, aminoacid, glycoside, polysaccharide, volatile oil, polypeptide, alkaloid.The present invention adopts conventional method to mix or make oral respectively or external preparation has certain curative effect to skin allergic disease alpine yarrow herb effective parts and Herba Achilleae effective ingredient.
Summary of the invention
The object of the invention is, the purposes of a kind of alpine yarrow herb effective parts and composition antianaphylaxis thereof is provided, adopt conventional water extraction or alcohol extracting method or macroporous resin extraction or organic solvent extraction method to obtain alpine yarrow herb effective parts and effective ingredient Herba Achilleae, according to a conventional method the Herba Achilleae crude extract that extracts and alpine yarrow herb effective parts and Herba Achilleae effective ingredient are mixed or make oral and external preparation respectively.Be applied to treat alpine yarrow herb effective parts and composition thereof in the allergic diseases, alpine yarrow herb effective parts and effective ingredient can suppress anaphylactic type (I type) anaphylaxis to antianaphylaxis, can suppress cytotoxicity (II type) anaphylaxis and delayed hypersensitivity (IV type) again.Wherein the inhibitory action to type i allergic reaction is the strongest, and effect and amount increase and strengthen, and therefore, alpine yarrow herb effective parts and effective ingredient thereof have tangible anti-allergic effects.
Alpine yarrow herb effective parts of the present invention comprises that total flavones, total keto acid, amino acids, glycoside, polysaccharide, volatile oil, polypeptide and alkaloid and effective ingredient comprise 4 ', 5-dihydroxy-3,3 ', 6,7-tetramethoxy flavone; 5,3 '-dihydroxy-6,7,8,4 '-the tetramethoxy flavone; 5-hydroxyl-6,7,8,3 ', 4 '-the pentamethoxyl flavone; 5,3 '-dihydroxy-6,8,4 '-three kinds of flavone compounds of trimethoxy flavone and rupestonic acid, isorupestonic acid, rupestric acid, four kinds of keto acid compounds of aciphyllic acid, according to a conventional method the alpine yarrow herb effective parts that extracts and Herba Achilleae effective ingredient are mixed or make oral respectively and external preparation at preparation antianaphylaxis medicinal usage.
Alpine yarrow herb effective parts and effective ingredient thereof, adopt conventional water extraction or alcohol extracting method or macroporous resin extraction or organic solvent extraction method to obtain effective site and effective ingredient, wherein effective site comprises total flavones, total keto acid, amino acids, glycoside, polysaccharide, volatile oil, polypeptide and alkaloid, effective ingredient comprises flavone compound 4 ', 5-dihydroxy-3,3 ', 6,7-tetramethoxy flavone; 5,3 ' dihydroxy-6,7,8,4 '-the tetramethoxy flavone; 5-hydroxyl-6,7,8,3 ', 4 '-the pentamethoxyl flavone; 5,3 '-dihydroxy-6,8,4 '-three kinds of flavone compounds of trimethoxy flavone and and rupestonic acid, isorupestonic acid, rupestric acid, four kinds of keto acid compounds of aciphyllic acid, according to a conventional method the alpine yarrow herb effective parts that extracts and Herba Achilleae effective ingredient are mixed or make oral and external preparation respectively, be used for the treatment of the skin allergy disease, through the animal antianaphylaxis is observed, the proof said preparation can suppress anaphylactic type (I type) anaphylaxis, can suppress cytotoxicity (II type) anaphylaxis and delayed hypersensitivity (IV type) again.Wherein the inhibitory action to type i allergic reaction is the strongest, and effect and amount increase and strengthen, and therefore, alpine yarrow herb effective parts and composition thereof are the good medicine of treatment allergic diseases.
The specific embodiment
Embodiment
Adopt conventional water extraction or alcohol extracting method or macroporous resin extraction or organic solvent extraction method to obtain alpine yarrow herb effective parts and comprise that total flavones, total keto acid, amino acids, glycoside, polysaccharide, volatile oil, polypeptide and alkaloid and effective ingredient comprise flavone compound 4 ', 5-dihydroxy-3,3 ', 6,7-tetramethoxy flavone; 5,3 '-dihydroxy-6,7,8,4 '-the tetramethoxy flavone; 5-hydroxyl-6,7,8,3 ', 4 '-the pentamethoxyl flavone; 5,3 '-dihydroxy-6,8,4 '-three kinds of flavone compounds of trimethoxy flavone and and rupestonic acid, isorupestonic acid, rupestric acid, four kinds of keto acid compounds of aciphyllic acid, according to a conventional method the alpine yarrow herb effective parts that extracts and Herba Achilleae effective ingredient are mixed or make oral and external preparation medicinal usage respectively in the preparation antianaphylaxis.
Alpine yarrow herb effective parts and effective ingredient thereof are that Uigur Medical Inst., Uigur Autonomous Region, Xinjiang provides.Now effective site and effective ingredient method and the result to animal anaphylaxis inhibition test is reported as follows:
One, the influence of homology models of passive skin irritability of rats reaction (PCA)
Material Wistar rat, Xinjiang Uygur Autonomous Regions's Experimental Animal Center provides.Alpine yarrow herb effective parts and effective ingredient thereof, Uygur medicine research in Xinjiang Uygur Autonomous Regions's provides.The Radix Trichosanthis powder, pharmacology teaching and research room of preclinical medicine institute of Beijing Medical University provides.Gel aluminum hydroxide, hua-shan pharmaceutical factory product.
Method and result get 5 male rats, the every sufficient sole of the foot injection 5mg/ml Radix Trichosanthis of every Mus-gel aluminum hydroxide suspension 0.1ml, and every Mus 4 foots are injected 0.4ml altogether.After 15 days, heart extracting blood, centrifuging and taking (resisting) serum.With preceding with antiserum with 1: 10 (antiserum: normal saline) dilution.Other gets 35 of rats, body weight 200 ± 20g, the male and female dual-purpose, be divided into the preparation group of blank group (NS), positive controls (sodium cromoglicate) and 3 various dose, 7 every group at random, in every Mus back depilation place intradermal injection antiserum sensitization, totally 3 points, every some 0.1ml, the 0.5% azovan blue 1ml that iv contains the 5mg Radix Trichosanthis after 48 hours attacks.Successive administration is 3 days before antigen is attacked, and every day is once oral.Antigen was attacked back 30 minutes, the sacrificed by decapitation rat, the skin locus coeruleus cut place in vitro, the KOH1ml that adds 1mol/L, 37 ℃ digested 15 hours, add 0.2mol/L phosphoric acid and acetone mixed liquor 3.75ml then after concussion, centrifugal 10 minutes of 2500r/min, (λ=610nm) measures absorbance (A) to get supernatant 721-type spectrophotometer.As a result, the alpine yarrow herb effective parts of 3 dosage and composition thereof all obviously suppress the influence (table 1) of PCA reaction.
The influence that table 1 alpine yarrow herb effective parts and effective ingredient thereof react homology P of Rats CA (mean ± SD)
| Group | Dosage | n | The PCA reaction | Suppression ratio |
| Matched group | 2.50 | 7 | 0.73±0.13 | |
| The sodium cromoglicate group (iv) | 15mg/kg | 7 | 0.00±0.00 *** | 100.00 |
| Alpine yarrow herb effective parts and one-tenth grouping thereof | 1.25 1.50 5.00 | 7 7 7 | 0.45±0.16 * 0.16±0.04 *** 0.06±0.02 *** | 38.8 78.1 91.0 |
Annotate: compare * * * P＜0.01, * P＞0.05 with matched group
As a result, alpine yarrow herb effective parts and effective ingredient thereof be in sensitization administration in preceding 1 day, and totally 3 days, can obviously suppress the reaction of homology models of passive skin irritability of rats, its effect strengthens with dosage.Show that said preparation has the obvious suppression effect to type.
Two, the influence of the antibody-mediated mast cell degranulation of rat allogenic cell
Material: the Wistar rat, Xinjiang Uygur Autonomous Regions's Experimental Animal Center provides.Alpine yarrow herb effective parts and composition thereof, Uygur medicine research in Xinjiang Uygur Autonomous Regions's provides.The Radix Trichosanthis powder, pharmacology teaching and research room of preclinical medicine institute of Beijing Medical University provides.Gel aluminum hydroxide, hua-shan pharmaceutical factory product.
Method and result: get 5 of rats, prepare rat anti Radix Trichosanthis serum by an above-mentioned method.Other gets 5 of rats, preparation rat mesentery specimen, and 10 of every Mus, every is 1cm
2It is standby to put into desk-top nutritional solution.Get 40 in test tube, each pipe adds desk-top nutritional solution 2ml, 1 of mesentery specimen and rat anti Radix Trichosanthis serum 0.2ml, and 37 ℃ of water-bath incubations made mesentery mastocyte sensitization after 30 minutes, washed specimen 3 times with 37 ℃ of desk-top nutritional solutions then, to remove the residue antiserum.Add 37 ℃ of desk-top nutritional solution 2ml again, 37 ℃ of water-bath incubations are pressed the dosing in pipe of dosage shown in the table 2 after 10 minutes, continued incubation 10 minutes, add Radix Trichosanthis antigen 0.1ml (containing Radix Trichosanthis 110ug) and attack, 37 ℃ of water-baths continue incubation after 20 minutes, with the specimen formaldehyde fixed, toluidine blue and Yihong dyeing, the hydrochloride alcohol color separation, dehydration of alcohol, the transparent and mounting of dimethylbenzene.With microscopic examination and count the mast cell degranulation situation, between organizing with its degranulated percentage rate relatively.What as a result, the preparation of variable concentrations can obviously suppress rat hypertrophy cell takes off granule reaction (table 2).
Table 2 alpine yarrow herb effective parts and effective ingredient thereof are to the influence of the antibody-mediated mast cell degranulation of rat allogenic cell (mean ± SD)
| Group | Concentration (ml/2.1ml) in the pipe | n | Take off granule (%) | Suppression ratio (%) |
| Matched group | 0.2 | 12 | 71.7±8.2 | |
| The sodium cromoglicate group (iv) | 50ug/ml | 10 | 34.2±9.6 *** | 100.00 |
| Alpine yarrow herb effective parts and one-tenth grouping thereof | 0.1 0.2 | 10 10 | 40.1±4.5 *** 34.0±6.9 *** | 38.8 78.1 |
Annotate: compare with matched group
* *P＜0.01
The result, antibody-mediated mast cell granule reaction has inhibitory action to the rat allogenic cell for alpine yarrow herb effective parts and effective ingredient thereof, show its anti-allergic effects and stable mastocyte, the mast cell degranulation reaction is reduced, and then reduce histamine and waited sensitive media release relevant.
Three, the influence that capillary permeability increases due to the histamine
Material: the Wistar rat, Xinjiang Uygur Autonomous Regions's Experimental Animal Center provides; Alpine yarrow herb effective parts and effective ingredient thereof, Uygur medicine research in Xinjiang Uygur Autonomous Regions's provides; Histamine phosphate is available from Shanghai Inst. of Biochemistry, Chinese Academy of Sciences.
Method and result: get 24 of rats, body weight 200 ± 20g, the male and female dual-purpose is divided into the preparation group of matched group (NS), Dexamethasone group and 2 various dose at random.Gastric infusion, once-a-day, totally 3 days, rat back depilation place in 1.5 hours after the last administration, intradermal injection histamine phosphate normal saline solution 0.1ml (including histamine 50ug), every Mus 2 points, iv0.5% azovan blue solution 1ml simultaneously.Sacrificed by decapitation rat after 30 minutes is cut the skin locus coeruleus, places in vitro, measures the absorbance (A) that skin should be put by each side by the PCA method, organizes a mean t check with its A value.As a result, the preparation of various dose can obviously suppress the vascular permeability increase effect (table 3) that histamine causes.
The influence that table 3 alpine yarrow herb effective parts and composition thereof increase capillary permeability due to the histamine (mean ± SD)
Annotate: compare with matched group
* *P＜0.01
As a result, alpine yarrow herb effective parts and effective ingredient thereof, successive administration 3 days has obvious inhibitory action to capillary permeability increase due to the histamine.
Four, the alpine yarrow herb effective parts preparation is to the influence of Forssman cutaneous vasculitis reaction
Material: Cavia porcellus, Xinjiang Medical College animal housing provides.Alpine yarrow herb effective parts and effective ingredient thereof, Uygur medicine research in Xinjiang Uygur Autonomous Regions's provides.
Method and result: under the aseptic condition, get blood, place in the aseptic bottle of phosphate buffer, put 4 ℃ of refrigerators and preserve standby from the sheep external jugular vein.Face with preceding with sheep blood 5ml in the 10ml centrifuge tube, 2000 rev/mins centrifugal 5 minutes, the WBC layer at supernatant discarded and interface is with physiological saline solution washing 3 times, after the washing, be made into 5% sheep erythrocyte suspension with physiological saline solution and (be equivalent to 1 * 109 cell/ml) approximately for the third time.Get 3 of male rabbits, vein 109SBBC suspension, each 1ml, 1 time every other day, totally 10 times, in back 14 days of last 1 injection, collect rabbit anteserum, this serum is anti-SBBC serum.
Get 30 of Cavia porcelluss, body weight 365 ± 41g, the male and female dual-purpose is divided into matched group (NS) at random, the alpine yarrow herb effective parts of Dexamethasone group and 3 various dose and become grouping, gastric infusion, every day 1 time, totally 2 days.Guinea pig back cropping in 1 hour after the last administration, the anti-SBBC serum of intradermal injection dilution in 1: 8, every some 0.1ml, totally 3 points, quiet notes 0.5% azovan blue 1ml after 24 hours, put to death Cavia porcellus after 30 minutes, the skin locus coeruleus is cut, place in vitro, measure the absorbance (A) that skin should be put by each side by the PCA method, organize a mean t check with its A value, the result, the preparation of various dose can obviously suppress Forssman cutaneous vasculitis reaction (table 4).
The influence that table 4 alpine yarrow herb effective parts and composition thereof react the Forssman cutaneous vasculitis (mean ± SD)
Annotate: compare with matched group
* *P＜0.01
As a result, alpine yarrow herb effective parts and effective ingredient thereof were administration the previous day of injection antiserum, totally 2 days.Can obviously suppress the reaction of Forssman cutaneous vasculitis, show that said preparation has inhibitory action significantly to II allergic reaction type (cytotoxicity allergy).
Five, the influence of the inductive delayed hypersensitivity of dinitrochlorobenzene
Material: the NIA mice, Xinjiang Medical College animal housing provides; Alpine yarrow herb effective parts and composition thereof, Uygur medicine research in Xinjiang Uygur Autonomous Regions's provides; 2,4 dinitrochlorobenzene, Shanghai reagent one factory's product.
Method and result: get 45 of mices, body weight 20 ± 1.7g, the male and female dual-purpose is divided into matched group (NS) at random, the preparation group of cyclophosphamide group and 3 various dose, 9 every group, gastric infusion, every day 1 time, totally 8 days, after administration the 4th day, the cropping of every Mus abdominal part, the about 3 * 3cm of area
2, 50ul evenly is applied in cropping place sensitization of skin with 1% dinitrochlorobenzene (DNFB) solution.After the sensitization the 5th day, 1%DNFB solution 50ul evenly is applied in the mouse right ear two sides carries out antigen and attack, after 24 hours, pull cervical vertebra and put to death mice, cut left and right sides auricular concha, take off the auricle of diameter 8mm with card punch, weigh.The difference of left and right sides auricle weight is the swelling degree, the result, and the alpine yarrow herb effective parts of various dose and effective ingredient thereof can obviously suppress the inductive delayed hypersensitivity of dinitrochlorobenzene (table 5).
Table 5 alpine yarrow herb effective parts and composition thereof are to the influence of the inductive delayed hypersensitivity of dinitrochlorobenzene (mean ± SD)
Annotate: compare with matched group
* *P＜0.01
As a result, alpine yarrow herb effective parts and effective ingredient thereof be in sensitization administration in preceding 3 days, and totally 8 days, can obviously suppress the inductive delayed hypersensitivity of dinitrochlorobenzene, its effect dosage increases and strengthens, and shows that said preparation also has obvious inhibitory action to the IV allergic reaction type.
Six, the influence that rabbit Arthus is reacted
Material: rabbit is planted by New Zealand, and Xinjiang Medical College animal housing provides; Alpine yarrow herb effective parts and effective ingredient thereof, Uygur medicine research in Xinjiang Uygur Autonomous Regions's provides; Ovalbumin is available from biochemistry portion of east Instrument and Equipment Company of the Chinese Academy of Sciences.
Method and result: get 25 of rabbit, body weight 2.1 ± 1.5kg, the male and female dual-purpose, be divided into matched group (NS) at random, the Herba Achilleae group of Dexamethasone group and three various dose, each treated animal CEA and Fu Shi Freund's complete adjuvant suspension 1ml, intramuscular injection, 1 time weekly, totally 4 weeks, last injection back rabbit back cropping in the 9th day, cropping place intradermal injection 10% ovalbumin was attacked in the 10th day, every some 0.2ml, totally 3 points, every day 1 time before antigen is attacked, gastric infusion is 4 days continuously, and antigen is attacked observation attack in 2,5 and 24 hours position, back and had or not red and swollen or hemorrhage, and according to the form below carries out extent of reaction classification and scoring, after averaging, carry out the statistics inspection between group, result, alpine yarrow herb effective parts and effective ingredient thereof every day 1 time before antigen is attacked, gastric infusion is 4 days continuously, and reaction does not have inhibitory action (table 6 to rabbit Arthus, table 7)
Table 6 extent of reaction standard grading and scoring
The influence that table 7 alpine yarrow herb effective parts and composition thereof react rabbit Arthus (mean ± SD)
Annotate: compare with matched group
* *P＜0.01,
*P＞0.05
Brief summary and discussion:, illustrate that alpine yarrow herb effective parts and composition thereof have following effects: 1. obviously suppress the reaction of homology models of passive skin irritability of rats according to The above results; 2. administration in vitro can significantly suppress the rat parent and takes off a reaction with the mesentery mastocyte of cell antibody mediation; 3. capillary permeability increase due to the histamine there is obvious inhibitory action; 4. reaction has inhibitory action to the Forssman cutaneous vasculitis; 5. to the inductive delayed hypersensitivity of dinitrochlorobenzene, inhibitory action is significantly arranged.
By influence to homology models of passive skin irritability of rats reaction (PCA), the influence of the mast cell degranulation that the rat allogenic cell is antibody-mediated, the influence that capillary permeability increases due to the histamine, the influence of Forssman cutaneous vasculitis reaction, five antianaphylaxis that influence of the inductive delayed hypersensitivity of dinitrochlorobenzene are observed, the proof said preparation can suppress anaphylactic type (I type) anaphylaxis, can suppress cytotoxicity (II type) anaphylaxis and delayed hypersensitivity (IV type) again.Wherein the inhibitory action to type i allergic reaction is the strongest, and effect and amount increase and strengthen, and therefore, alpine yarrow herb effective parts and effective ingredient thereof are the good medicine of treatment allergic diseases.
Claims (1)
1. an alpine yarrow herb effective parts comprises that total flavones, total keto acid, amino acids, glycoside, polysaccharide, volatile oil, polypeptide and alkaloid and effective ingredient comprise 4 ', 5-dihydroxy-3,3 ', 6,7-tetramethoxy flavone; 5,3 '-dihydroxy-6,7,8,4 '-the tetramethoxy flavone; 5-hydroxyl-6,7,8,3 ', 4 '-the pentamethoxyl flavone; 5,3 '-dihydroxy-6,8,4 '-three kinds of flavone compounds of trimethoxy flavone and rupestonic acid, isorupestonic acid, rupestric acid, four kinds of keto acid compounds of aciphyllic acid, according to a conventional method the alpine yarrow herb effective parts that extracts and Herba Achilleae effective ingredient are mixed or make oral and external preparation purposes respectively at preparation antianaphylaxis medicine.
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| JP2007314468A (en) * | 2006-05-25 | 2007-12-06 | Meiji Milk Prod Co Ltd | Endoplasmic reticulum stress control compound and pharmaceutical composition comprising the same |
| CN103127196A (en) * | 2013-03-18 | 2013-06-05 | 中国科学院新疆理化技术研究所 | Preparation method and application of total extractive of artemisia rupestris L |
| CN103570739A (en) * | 2013-11-01 | 2014-02-12 | 湖南科源生物制品有限公司 | Method for separating arteannuic acid from artemisia annua essential oil |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1164314C (en) * | 2002-04-29 | 2004-09-01 | 斯拉甫·艾白 | Yizhi Artemisia flavonoids capsules |
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| CN1164314C (en) * | 2002-04-29 | 2004-09-01 | 斯拉甫·艾白 | Yizhi Artemisia flavonoids capsules |
Non-Patent Citations (3)
| Title |
|---|
| 新疆一枝蒿提取物保肝作用的实验研究. 斯拉甫，阿不都热依木，古力娜，努尔买买提.中国中西医结合杂志，第22卷第2期. 2002 * |
| 维吾尔药一枝蒿的应用简介. 阿丽艳，古力米热.中国民族医药杂志，第2期. 2004 * |
| 维药一枝蒿抗过敏作用的实验研究. 斯拉甫，古力娜，刘发，陈玮，郭美玲，付尔康.中国民族医药杂志，第2卷第2期. 1996 * |
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