CN100374546C - Industrial scale preparation method of rhodobacter sphaeroides, fermentation liquor and application thereof - Google Patents

Abstract

The invention discloses a method for producing a multifunctional fermentation liquid by bacterial fermentation, in particular to a method for producing a functional fermentation liquid by rhodobacter sphaeroides. The preservation number of the strain is China general microbiological culture Collection center (CGMCC) No. 1562. Preservation time: 12/2005. The method comprises the steps of carrying out amplification culture on a first-stage seeding tank (200L) and a second-stage seeding tank (2000L) on strains which are separated from a culture dish and strengthened by shaking bottles, and transferring the strains to a 5000-20000L large-scale fermentation tank for large-scale fermentation in the same way.

Description

Industrial scale preparation method of Rhodobacter sphaeroides and its fermentation broth and application

technical field

The invention relates to a production method for preparing a functional fermented liquid by bacterial fermentation, in particular to a production method for preparing a functional fermented liquid by using Rhodobacter sphaeroides.

Background technique

At present, the research on Rhodobacter sphaeroides is limited to the laboratory level, and there is no technical scheme using Rhodobacter sphaeroides for industrial-scale submerged fermentation. At the same time, in the beverage industry, there is a lack of a beverage that can greatly promote the health of the human body produced by bio-fermentation technology.

Contents of the invention

The object of the present invention is to provide a kind of fermenting method that utilizes Rhodobacter sphaeroides to carry out deep layer industrialization scale, the fermented liquid that obtains is beneficial to the health of human body, can regulate the immune system of human body, increases the resistance of human body .

In the inventor's series of patents (China CN1184307C), a similar fermentation method was involved, but after several years of continuous testing by the inventor, he optimized the composition of the fermentation broth and optimized the fermentation conditions, resulting in better results, Lower cost fermentation broth.

The present invention utilizes the method for industrial scale fermentation of Rhodobacter sphaeroides, the method is that Rhodobacter sphaeroides (Rhodobacter sphaeroides) is successively passed through a 500ml Erlenmeyer flask, a 50-200L primary seed tank, a 500-2000L secondary seed tank and a 5000- 20000L large fermentation tank deep fermentation,

The fermentation conditions are respectively:

500ml Erlenmeyer flask: keep the temperature at 25-35°C, illuminate with a 300LX incandescent light, and carry out 24 hours at the speed of the shaker at 50-60 rpm;

200L seed tank: pH 6.4-7.5, temperature 25-35°C, use 300LX incandescent lamps to illuminate through two mirrors with different angles, ferment at a constant temperature of 25-35°C for 24 hours, pass through the primary seed tank (50-200L) and Second-level seed tanks (500-2000L) were expanded for 24 hours, and the cultivation temperature, pH value, light and ventilation pressure were the same as those of the first-level seed tanks;

The pressure of the primary and secondary seed tanks is 0.04Mpa;

The fermentation conditions of the large-scale fermentation tank are as follows: 300-500LX incandescent light is illuminated through three different angles of sight glass, the pH of the feed liquid is 6.4-7.5, the inlet pressure is 0.1-0.3Mpa, and the pressure in the tank is adjusted to 0.05-0.08Mpa. Control the temperature at 25-35°C and ferment for 24 hours;

Promptly obtain fermented liquid, it is characterized in that: described Rhodobacter sphaeroides is preserved in China Microorganism Strain Collection Management Committee General Microorganism Center, numbering CGMCC No.1562, and the preparation method of described cultured liquid is:

Proportion of inorganic salt solution in 1000ml distilled water;

MgSO ₄ 7H ₂ O 0.2-3.0 g

CaCl·2H ₂ O 0.1-1.0g

FeSO ₄ 7H ₂ O 0.1-1.0 g

NaEDTA 0.1-1.0g

Proportion of trace element solution in 250ml distilled water;

H ₃ BO ₄ 0.5-1.5 g

_MnSO4 50-200mg

NaMoO ₄ 20-100 mg

ZnSO ₄ 10-20 mg

Cu(NO ₃ ) ₂ 2-3 mg

Sodium molybdate 6-15 mg

Sodium selenite 6-15 mg;

Proportion of growth factor solution in 100ml distilled water

Biotin 0.2-0.5 mg

Vitamin B ₁ 10-30 mg

Niacin 10-30mg

p-aminobenzoic acid 10-20 mg;

Lactic acid solution ratio

Distilled water 900ml

Lactic acid 30-100ml, adjust the pH to 6.4 with 12N NaOH solution;

Ammonium sulfate solution ratio

Distilled water 800ml

Ammonium sulfate 3-8 grams

Make up to 1000ml with water;

After dissolving, add distilled water to 1000 ml, then adjust pH to 6.4 with 12N NaOH solution

Phosphate buffer

K ₂ HPO ₄ 15-50 g

KH ₂ PO ₄ 10-15 g

Make up to 1000ml with water;

Take respectively:

a. Inorganic salt solution 10-30%,

b. Trace element solution 0.1-1.0%

c. Growth factor solution 0.1%

d. Lactic acid solution 2-10%

e. Ammonium sulfate solution 0.5-2%

f. Phosphate buffer 1-5%

Mix the proportions a-f according to the above percentages, and the rest is water to obtain the culture solution. When making slant test tube or petri dish culture medium, additionally add: peptone 1%, yeast extract 0.2%. Preferred:

Proportion of inorganic salt solution in 1000ml distilled water;

MgSO ₄ 7H ₂ O 0.3 g

CaCl 2H ₂ O 0.05 g

FeSO ₄ ·7H ₂ O 0.5 g

NaEDTA 0.5 g

Proportion of trace element solution in 250ml distilled water;

_{H3BO4 1.0} g

_MnSO4 120mg

NaMoO ₄ 60 mg

ZnSO4 15 mg

Cu(NO ₃ ) ₂ 2 mg

Sodium molybdate 12mg

Sodium selenite 12 mg;

Proportion of growth factor solution in 100ml distilled water

Biotin 0.5 mg

Vitamin _B1 10 mg

Niacin 10mg

p-aminobenzoic acid 10 mg;

Lactic acid solution ratio

Distilled water 900ml

Lactic acid 40ml, adjust the pH to 6.4 with 12N NaOH solution;

Ammonium sulfate solution ratio

Distilled water 800ml

Ammonium sulfate 5 grams

Make up to 1000ml with water;

After dissolving, add distilled water to 1000 ml, then adjust pH to 6.4 with 12N NaOH solution

Phosphate buffer

K ₂ HPO ₄ 40g

KH ₂ PO ₄ 10 g

Make up to 1000ml with water;

Take respectively:

a. Inorganic salt solution 20%,

b. Trace element solution 0.2%

c. Growth factor solution 0.1%

d. Lactic acid solution 5%

e. Ammonium sulfate solution 1.5%

f. Phosphate buffer 3%

Mix the proportions a-f according to the above percentages, and the rest is water to obtain the culture solution.

When making slant test tube or petri dish culture medium, additionally add: peptone 1%, yeast extract 0.2%.

The fermented liquid that obtains with above-mentioned method has following purposes:

It is used to prepare bio-drinks, wherein the fermented liquid is mixed with purified water, and food additives are added to adjust the taste.

Root rot can be cured by irrigating the root rot with 5-10 times the fermentation liquid.

Spraying fruits and raw vegetables with 50-100 times of fermented liquid described in claim 3 can improve the SOD of plant fruits.

Use 100-150 times of the fermented liquid described in claim 3 as live bacteria biological material.

Adding 3-5ppm of pond water to fish, shrimp or crab ponds will purify water quality, reduce diseases and increase growth rate.

Fermentation liquid and water are added to the feed or drinking water of cows, pigs and chickens according to the ratio of 1:5-10.

The frequency and dosage of the above fermented liquid are 1-3 times a day, and the effect is obvious for a period of use of more than 1 month.

Beneficial effect:

The fermentation time is shortened, the production cost is low, the use effect is good, and a very good effect has been obtained in practical application.

The specific application and test results are as follows:

Testing unit: Institute of Microbiology, Chinese Academy of Sciences;

(2004) Weijianzi No. 095 Testing and Appraisal Person in charge: Zhou Yuguang

A variety of nutritional components needed by plants are found, including the components necessary for the growth of multiple plants, and the sensitivity test between the product of the present invention and 42 kinds of commonly used drugs is also detected.

Testing unit: Yantai Institute of Agricultural Sciences, Shandong Province;

The person who completed the experiment: Jiang Xueling

Name: Summary report on the test of plant growth fungicides on cucumber

Results: After the control group experiment, we got:

The first group: net income per mu increased by 766.5 yuan to 995.25 yuan

The second group: the net income per mu increased by 566.5 yuan to 916.8 yuan (obtained from the per mu yield and the standard market price of the year). The input-output ratios were: 1:2.83, 1:8.48, 1:61.12.

Cucumber yields increased by more than 10% with different application methods.

The application method is: subsurface flushing combined with foliar spraying or foliar spraying alone. The fermentation broth was diluted 100 times.

Testing unit: Yantai Institute of Agricultural Sciences, Shandong Province;

The person who completed the experiment: Jiang Xueling

Name: Summary report on the test of plant growth agent on pepper

Results: After the control group experiment, we got:

Yield increase: 5-10.7%

The input-output ratios are: 1:3.1, 1:2.9, 1:29.5.

If you invest 1 yuan in fermentation liquid, you can get 2.9 yuan in return, and you can get 29.5 yuan in return for foliar spraying.

The application method is: subsurface flushing combined with foliar spraying or foliar spraying alone. The fermented liquid was diluted 100 times and sprayed on the leaves, ensuring more than 3 consecutive times.

Testing unit: Binzhou Soil and Fertilizer Workstation;

Test completed by: Sun Fulai

Name: Summary report on the test of plant growth fungicides on cucumber

Time 2005.4.16 to 2005.7.25

Results: After the control group experiment, we got:

The net income per mu increased by 436.24 yuan to 754.565 yuan (obtained from the yield per mu and the standard market price of the year). The input-output ratios were: 1:2.18, 1:6.76, and 1:50.3.

Cucumber yields increased by more than 10% with different application methods.

The application method is: subsurface flushing combined with foliar spraying or foliar spraying alone. The fermentation broth was diluted 100 times.

Testing unit: Binzhou Soil and Fertilizer Workstation;

Test completed by: Sun Fulai

Name: Summary report on the test of plant growth agent on pepper

Time 2005.4.16 to 2005.7.31

Results: After the control group experiment, we got:

The input-output ratios are: 1:2.9, 1:2.6, 1:25.8.

Different application methods can increase the yield of pepper by more than 5%.

The detection is harmless to humans and animals.

The application method is: subsurface flushing combined with foliar spraying or foliar spraying alone. The fermentation broth was diluted 100 times.

Inspection report (Central Laboratory of Shandong Academy of Agricultural Sciences)

It can degrade the following three pesticides: dichlorvos, beta-cypermethrin, methamidophos EC, and chlorpyrifos, and the effect is very obvious.

Source of bacteria:

In the mud of the upper section of the Songjia River in Cunliji Town, Penglai City, Shandong Province.

Culture preservation number:

CGMCC No.1562, General Microbiology Center, China Committee for Culture Collection of Microorganisms.

Preservation time: December 12, 2005.

Separation method:

Take 1 liter of mud in the river from July to August, put 10 liters of the culture solution prepared according to the above method into 22 500ml Erlenmeyer flasks, plug the bottles with gauze wrap cotton plugs, wrap them with old newspapers for external use, and wrap them with paper ropes. Tie it tightly, put it in a high-temperature sterilization pot and heat it to 121°C for 30 minutes to sterilize, then lower it to 35°C. Slowly and evenly divide the sludge into 22 bottles of culture solution while stirring in the sterile room. Then use 10% lactic acid solution to adjust the pH to 6.4-7.5, plug it with the cotton plug on the original bottle, wrap the newspaper and tie it tightly with paper rope, place it on a constant temperature shaker at 25-35°C, and the shaker speed is 50-60 per minute. Turn and shake, and illuminate with incandescent light at the same time, the light requirement is 100LX per square meter. After cultivating on a shaker for 40 hours, take the culture solution with a red reaction (take the upper layer), transfer it into a culture dish (prepared according to the above-mentioned medium formula) with a pipette in the inoculation chamber, and cultivate it under the above-mentioned culture conditions for one week, and then Then take the colonies with larger red dots from the petri dish in the aseptic room and insert them into the same culture solution as above, and enter into the strengthening culture. In this way, repeated dozens of times of separation culture, strengthening, sampling and microscopic examination, to achieve no bacteria The pure species can have a fermentation index of more than 5 billion per milliliter in 24 hours, and the absorbance value measured at a wavelength of 525-530 nm with a spectrophotometer is 1.50, which can be used as a production species.

production method:

Put the test tube or petri dish bacteria into the culture medium of 500ml Erlenmeyer flask in the aseptic room (the culture medium formula in the Erlenmeyer flask is the same as above, the disinfection, temperature and pH value are the same as above), place it on a shaker at a constant temperature of 25-35°C, and use 300LX Illuminated by incandescent light, the shaking table rotates at 50-60 rpm for 24 hours, and when the sampling detection is more than 5 billion/ml, use a sterilized inoculator to insert a 50-200L seed tank, and the inoculation volume should be greater than 10%. The formula of the culture solution is the same as above. It is advisable to measure the pH of 6.4-7.5 after the preparation of the culture solution. The pH is adjusted with lactic acid solution or 12N NaOH solution, and the disinfection temperature is 121 ° C for 30 minutes. Then pass cold water to the jacket of the tank to inoculate when the temperature is lowered to 35°C. After the inoculation is completed, the compressed air after filtration and sterilization is introduced from the bottom of the tank for stirring. The temperature in the tank is 25-35°C, and the air inlet pressure is 0.1- 0.3Mpa, the pressure inside the tank is about 0.03-0.08Mpa. Also use incandescent lamps to illuminate the 300LX through two mirrors with different angles. The adjustment of the pressure inside the tank is controlled from the vent valve on the top of the tank. After fermenting at a constant temperature of 25-35°C for 24 hours, transfer to the secondary seed tank through the connection pipeline between the first-level seed tank (200L) and the second-level seed tank (2000L) according to the above method (the culture medium of the second-level seed tank and the first-level seed tank The same as the first-level seed tank) to expand the culture for 24 hours, the culture temperature, pH value, light and ventilation pressure are the same as the first-level seed tank, after the second-level seed tank fermentation is completed, transfer to 10000L large-scale fermentation in the same way Scale fermentation in tanks. The formula of the base material in the large-scale fermenter is the same as the above, except that the distilled water used is changed to membrane-filtered water. The conditions for transferring to the large-scale fermenter are as follows: 1000LX of incandescent light is illuminated through three different angles of sight glass, and the feed liquid pH6.4-7.5, air inlet pressure 0.3Mpa, adjust tank pressure 0.05-0.08Mpa, control temperature 35°C, ferment for 24 hours, take samples every 2 hours, stop fermentation immediately when bacteria are found during fermentation, and the finished product of fermentation Liquid is 40-50 billion/ml. The obtained fermented liquid is the functional oral liquid of the present invention, which is used for agriculture, aquaculture and poultry and livestock.

Example 1

The preparation method of fermentation liquid culture liquid:

Proportion of inorganic salt solution in 1000ml distilled water;

MgSO ₄ 7H ₂ O 0.3 g

CaCl 2H ₂ O 0.05 g

FeSO ₄ ·7H ₂ O 0.5 g

NaEDTA 0.5 g

Proportion of trace element solution in 250ml distilled water;

_{H3BO4 1.0} g

_MnSO4 120mg

NaMoO ₄ 60 mg

ZnSO ₄ 15mg

Cu(NO ₃ ) ₂ 2 mg

Sodium molybdate 12 mg

Sodium selenite 12 mg;

Proportion of growth factor solution in 100ml distilled water

Biotin 0.5 mg

Vitamin _B1 10 mg

Niacin 10 mg

p-aminobenzoic acid 10 mg;

Lactic acid solution ratio

Distilled water 900ml

Lactic acid 40ml, adjusted to pH 6.4 with 12N NaOH solution;

Ammonium sulfate solution ratio

Distilled water 800ml

Ammonium sulfate 5 grams

Make up to 1000ml with water;

After dissolving, add distilled water to 1000 ml, then adjust pH to 6.4 with 12N NaOH solution

Phosphate buffer

K ₂ HPO ₄ 40g

KH ₂ PO ₄ 10 g

Make up to 1000ml with water;

Take respectively:

a. Inorganic salt solution 20%,

b. Trace element solution 0.2%

c. Growth factor solution 0.1%

d. Lactic acid solution 5%

e. Ammonium sulfate solution 1.5%

f. Phosphate buffer 3%

The proportions a-f are mixed according to the above weight percentage, and the rest is water to obtain the culture solution.

When making slant test tube or petri dish culture medium, additionally add: peptone 1%, yeast extract 0.2%.

Example 2

Proportion of inorganic salt solution in 1000ml distilled water;

MgSO ₄ 7H ₂ O 3.0 g

CaCl·2H ₂ O 1.0 g

FeSO ₄ 7H ₂ O 1.0 g

NaEDTA 1.0 g

Proportion of trace element solution in 250ml distilled water;

_{H3BO4 1.5} g

_MnSO4 200 mg

NaMoO ₄ 100 mg

_ZnSO4 20 mg

Cu(NO ₃ ) ₂ 3 mg

Sodium molybdate 15mg

Sodium selenite 15 mg;

Proportion of growth factor solution in 100ml distilled water

Biotin 0.5mg

Vitamin _B1 30 mg

Niacin 30 mg

p-aminobenzoic acid 20 mg;

Lactic acid solution ratio

Distilled water 900ml

Lactic acid 100ml, adjust the pH to 6.4 with 12N NaOH solution;

Ammonium sulfate solution ratio

Distilled water 800ml

Ammonium sulfate 8 grams

Make up to 1000ml with water;

After dissolving, add distilled water to 1000 ml, then adjust pH to 6.4 with 12N NaOH solution

Phosphate buffer

_{K2HPO4 50g}

KH ₂ PO ₄ 15 g

Make up to 1000ml with water;

Take respectively:

a. Inorganic salt solution 30%,

b. Trace element solution 1.0%

c. Growth factor solution 0.1%

d. Lactic acid solution 10%

e. Ammonium sulfate solution 2%

f. Phosphate buffer 5%

The proportions a-f are mixed according to the above weight percentage, and the rest is water to obtain the culture solution.

When making slant test tube or petri dish culture medium, additionally add: peptone 1%, yeast extract 0.2%.

Example 3

Proportion of inorganic salt solution in 1000ml distilled water;

MgSO ₄ 7H ₂ O 0.2 g

CaCl·2H ₂ O 0.1 g

FeSO ₄ ·7H ₂ O 0.1 g

NaEDTA O.1 g

Proportion of trace element solution in 250ml distilled water;

_{H3BO4 0.5} g

_MnSO4 50mg

NaMoO ₄ 20 mg

ZnSO ₄ 10mg

Cu(NO ₃ ) ₂ 2 mg

Sodium molybdate 6 mg

Sodium selenite 6 mg;

Proportion of growth factor solution in 100ml distilled water

Biotin 0.2 mg

Vitamin _B1 10mg

Niacin 10 mg

p-aminobenzoic acid 10 mg;

Lactic acid solution ratio

Distilled water 900ml

Lactic acid 30ml, adjust the pH to 6.4 with 12N NaOH solution;

Ammonium sulfate solution ratio

Distilled water 800ml

Ammonium sulfate 3 grams

Make up to 1000ml with water;

After dissolving, add distilled water to 1000 ml, then adjust pH to 6.4 with 12N NaOH solution

Phosphate buffer

K ₂ HPO ₄ 15 g

KH ₂ PO ₄ 10 g

Make up to 1000ml with water;

Take respectively:

a. Inorganic salt solution 10%,

b. Trace element solution 0.1%

c. Growth factor solution 0.1%

d. Lactic acid solution 2%

e. Ammonium sulfate solution 0.5%

f. Phosphate buffer 1%

The proportions a-f are mixed according to the above weight percentage, and the rest is water to obtain the culture solution.

When making slant test tube or petri dish culture medium, additionally add: peptone 1%, yeast extract 0.2%.

Example 4

Proportion of inorganic salt solution in 1000ml distilled water;

MgSO ₄ 7H ₂ O 3.0 g

CaCl·2H ₂ O 0.1 g

FeSO ₄ 7H ₂ O 1.0 g

NaEDTA 1.0 g

Proportion of trace element solution in 250ml distilled water;

_{H3BO4 0.5} g

_MnSO4 200 mg

NaMoO ₄ 20 mg

ZnSO ₄ 10mg

Cu(NO ₃ ) ₂ 3 mg

Sodium molybdate 15 mg

Sodium selenite 6 mg;

Proportion of growth factor solution in 100ml distilled water

Biotin 0.2 mg

Vitamin _B1 30mg

Niacin 30 mg

p-aminobenzoic acid 10 mg;

Lactic acid solution ratio

Distilled water 900ml

Lactic acid 500ml, adjust the pH to 6.4 with 12N NaOH solution;

Ammonium sulfate solution ratio

Distilled water 800ml

Ammonium sulfate 5 grams

Make up to 1000ml with water;

After dissolving, add distilled water to 1000 ml, then adjust pH to 6.4 with 12N NaOH solution

Phosphate buffer

_{K2HPO4 30g}

KH ₂ PO ₄ 12 g

Make up to 1000ml with water;

Take respectively:

a. Inorganic salt solution 15%,

b. Trace element solution 0.5%

c. Growth factor solution 0.1%

d. Lactic acid solution 5%

e. Ammonium sulfate solution 1.5%

f. Phosphate buffer 3%

The proportions a-f are mixed according to the above weight percentage, and the rest is water to obtain the culture solution.

When making slant test tube or petri dish culture medium, additionally add: peptone 1%, yeast extract 0.2%.

Application of fermentation broth:

1. The fermented liquid is mixed with 3% Sophora japonica honey to make an oral solution for immune regulation and maintenance of intestinal function.

2. The fermented liquid obtained by fermenting the lotus seed extract and the secondary seeds is the hypoglycemic liquid for regulating blood sugar.

3. Adjust the taste with fermented liquid, purified water and food additives, which is the bio-drink.

4. The fermented liquid can be directly used in plant nutrient liquid such as pollution-free vegetables and fruits to produce SOD vegetables and fruits.

5. For the root rot of apple trees, cherry trees, apricot trees, pear trees and other fruit trees, the root rot can be cured by irrigating the rotten roots with 5-10 times the fermentation liquid.

6. Add fermented liquid and water to the feed or drinking water of cows, pigs, and chickens at a ratio of 1:5-10, which can increase the milk production rate of cows by 10-20%, improve the immunity of poultry and livestock, and reduce diseases , Accelerate growth.

7. Add 3-5ppm of pond water to ponds for fish, shrimp, crab and other aquatic products, which will purify water quality, reduce diseases and increase growth rate.

The product produced by the fermented liquid of the present invention has no side effect on human body, can regulate biological immune system, and is extremely beneficial to human health.

Claims (7)

1. A method utilizing Rhodobacter sphaeroides industrial scale fermentation, characterized in that: the method is followed by Rhodobacter sphaeroides through 500ml Erlenmeyer flask, 50-200L primary seed tank, 500-2000L Secondary seed tanks and 5000-20000L large-scale fermentation tanks for submerged fermentation, the fermentation conditions are as follows: 500ml Erlenmeyer flask: keep the temperature at 25-35°C, illuminate with 300LX incandescent light, shaker at 50-60 rpm for 24 hours: 200L seed tank: pH 6.4-7.5, temperature 25-35°C, use 300LX incandescent lamps to illuminate through two mirrors with different angles, ferment at a constant temperature of 25-35°C for 24 hours, pass through the primary seed tank (50-200L) and Second-level seed tanks (500-2000L) were expanded for 24 hours, and the cultivation temperature, pH value, light and ventilation pressure were the same as those of the first-level seed tanks; The pressure of the primary and secondary seed tanks is 0.04Mpa: The fermentation conditions of the large-scale fermentation tank are as follows: 300-500LX incandescent light is illuminated through three different angles of sight glass, the pH of the feed liquid is 6.4-7.5, the inlet pressure is 0.1-0.3Mpa, and the pressure in the tank is adjusted to 0.05-0.08Mpa. Control the temperature at 25-35°C and ferment for 24 hours; Promptly obtain fermented liquid, it is characterized in that: described Rhodobacter sphaeroides is preserved in China Microorganism Strain Collection Management Committee General Microorganism Center, numbering CGMCC No.1562, and the preparation method of described cultured liquid is: Proportion of inorganic salt solution in 1000ml distilled water; MgSO ₄ 7H ₂ O 0.2-3.0 g CaCl·2H ₂ O 0.1-1.0g FeSO ₄ 7H ₂ O 0.1-1.0 g NaEDTA 0.1-1.0g Proportion of trace element solution in 250ml distilled water; H ₃ BO ₄ 0.5-1.5 g _MnSO4 50-200 mg NaMoO ₄ 20-100 mg ZnSO ₄ 10-20 mg Cu(NO ₃ ) ₂ 2-3 mg Sodium molybdate 6-15 mg Sodium selenite 6-15 mg; Proportion of growth factor solution in 100ml distilled water Biotin 0.2-0.5 mg Vitamin B ₁ 10-30 mg Niacin 10-30 mg p-aminobenzoic acid 10-20 mg; Lactic acid solution ratio Distilled water 900ml Lactic acid 30-100ml, adjust the pH to 6.4 with 12N NaOH solution; Ammonium sulfate solution ratio 800ml of distilled water Ammonium sulfate 3-8 grams Make up to 1000ml with water; After dissolving, add distilled water to 1000 ml, then adjust pH to 6.4 with 12N NaOH solution Phosphate buffer K ₂ HPO ₄ 15-50 g KH ₂ PO ₄ 10-15 g Make up to 1000ml with water; Take respectively: a. Inorganic salt solution 10%-30%, b. Trace element solution 0.1%-1.0% c. Growth factor solution 0.1% d. Lactic acid solution 2%-10% e. Ammonium sulfate solution 0.5%-2% f. Phosphate buffer 1%-5% Mix the proportions a-f according to the above weight percentages, and the rest is water to obtain the culture solution; when making inclined test tube or culture dish culture medium, add: 1% peptone and 0.2% yeast extract. 2. utilize the method for rhodobacter sphaeroides industrial scale fermentation as claimed in claim 1, it is characterized in that: in: Proportion of inorganic salt solution in 1000ml distilled water; MgSO ₄ 7H ₂ O 0.3 g CaCl 2H ₂ O 0.05 g FeSO ₄ ·7H ₂ O 0.5 g NaEDTA 0.5 g Proportion of trace element solution in 250ml distilled water; _{H3BO4 1.0} g _MnSO4 120 mg NaMoO ₄ 60 mg ZnSO ₄ 15mg Cu(NO ₃ ) ₂ 2 mg Sodium molybdate 12 mg Sodium Selenite 12 mg; Proportion of growth factor solution in 100ml distilled water Biotin 0.5mg Vitamin _B1 10 mg Niacin 10mg p-aminobenzoic acid 10 mg; Lactic acid solution ratio Distilled water 900ml Lactic acid 40ml, adjust pH to 6.4 with 12N NaOH solution; Ammonium sulfate solution ratio Distilled water 800ml Ammonium sulfate 5 grams Make up to 1000ml with water; After dissolving, add distilled water to 1000 ml, then adjust pH to 6.4 with 12N NaOH solution Phosphate buffer K ₂ HPO ₄ 40g KH ₂ PO ₄ 10 g Make up to 1000ml with water; Take respectively: a. Inorganic salt solution 20%, b. Trace element solution 0.2% c. Growth factor solution 0.1% d. Lactic acid solution 5% e. Ammonium sulfate solution 1.5% f. Phosphate buffer 3% Mix the proportions a-f according to the above percentages, and the rest is water to obtain the culture solution; When making slant test tube or petri dish culture medium, add additionally; peptone 1%, yeast extract 0.2%. 3. A fermented liquid, characterized in that: it is produced by utilizing the method for industrial-scale fermentation of Rhodobacter sphaeroides described in claim 1. 4. The use of the fermented liquid as claimed in claim 3, characterized in that it is used to prepare a biological beverage, wherein after the fermented liquid is mixed with purified water, food additives are added to adjust the taste. 5. the purposes of fermented liquid as described in claim 3, it is characterized in that: use the fermented liquid described in claim 3 of 100-150 times as living bacteria biological material. 6. The purposes of fermented liquid as described in claim 3, it is characterized in that: in the pond of feeding fish, shrimp or crab, add by 3-5ppm of pond water quantity, can purify water quality, reduce disease, increase growth rate. 7. The use of the fermented liquid as claimed in claim 3, characterized in that: the fermented liquid and water are added to the feed or drinking water of cows, pigs, and chickens at a ratio of 1:5-10.