CH615947A5 - Process for the cultivation of anchorage-dependent cells in a microcarrier culture, microcarriers for carrying it out and use of the process. - Google Patents

Process for the cultivation of anchorage-dependent cells in a microcarrier culture, microcarriers for carrying it out and use of the process. Download PDF

Info

Publication number: CH615947A5
Authority: CH; Switzerland
Prior art keywords: microcarrier; microcarriers; cells; groups; beads
Prior art date: 1976-11-11

Application number

CH1382677A

Other languages

German (de)

English (en)

Inventor

David W Levine

William G Thilly

Daniel I C Wang

Jason S Wong

Original Assignee

Massachusetts Inst Technology

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1976-11-11

Filing date

1977-11-11

Publication date

1980-02-29

1977-11-11 Application filed by Massachusetts Inst Technology filed Critical Massachusetts Inst Technology

1980-02-29 Publication of CH615947A5 publication Critical patent/CH615947A5/de

Links

238000000034 method Methods 0.000 title claims description 70
230000001419 dependent effect Effects 0.000 title claims description 34
230000008569 process Effects 0.000 title description 15
239000011324 bead Substances 0.000 claims description 70
229920002307 Dextran Polymers 0.000 claims description 52
230000010261 cell growth Effects 0.000 claims description 45
239000002609 medium Substances 0.000 claims description 26
238000004113 cell culture Methods 0.000 claims description 21
230000012010 growth Effects 0.000 claims description 21
-1 diethylaminoethyl groups Chemical group 0.000 claims description 20
239000001963 growth medium Substances 0.000 claims description 15
238000004519 manufacturing process Methods 0.000 claims description 15
238000004873 anchoring Methods 0.000 claims description 13
239000000725 suspension Substances 0.000 claims description 11
125000003636 chemical group Chemical group 0.000 claims description 9
239000006227 byproduct Substances 0.000 claims description 8
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230000015572 biosynthetic process Effects 0.000 claims description 6
238000012258 culturing Methods 0.000 claims description 3
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VEXZGXHMUGYJMC-UHFFFAOYSA-M Chloride anion Chemical compound [Cl-] VEXZGXHMUGYJMC-UHFFFAOYSA-M 0.000 description 13
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SQGYOTSLMSWVJD-UHFFFAOYSA-N silver(1+) nitrate Chemical compound [Ag+].[O-]N(=O)=O SQGYOTSLMSWVJD-UHFFFAOYSA-N 0.000 description 2
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QLOKJRIVRGCVIM-UHFFFAOYSA-N 1-[(4-methylsulfanylphenyl)methyl]piperazine Chemical compound C1=CC(SC)=CC=C1CN1CCNCC1 QLOKJRIVRGCVIM-UHFFFAOYSA-N 0.000 description 1
WRYDGMWSKBGVHS-UHFFFAOYSA-N 2-bromo-n,n-diethylethanamine Chemical compound CCN(CC)CCBr WRYDGMWSKBGVHS-UHFFFAOYSA-N 0.000 description 1
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GELYHORWAXJBRV-UHFFFAOYSA-N 3-amino-3-(bromomethyl)pentane-1,5-diol Chemical compound OCCC(CBr)(N)CCO GELYHORWAXJBRV-UHFFFAOYSA-N 0.000 description 1
YMAPBXBQNXUXHG-UHFFFAOYSA-N 3-amino-3-(chloromethyl)pentane-1,5-diol Chemical compound OCCC(CCl)(N)CCO YMAPBXBQNXUXHG-UHFFFAOYSA-N 0.000 description 1
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239000004372 Polyvinyl alcohol Substances 0.000 description 1
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239000004809 Teflon Substances 0.000 description 1
229920006362 Teflon® Polymers 0.000 description 1
102000004142 Trypsin Human genes 0.000 description 1
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150000008044 alkali metal hydroxides Chemical class 0.000 description 1
150000001450 anions Chemical class 0.000 description 1
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QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
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YPHMISFOHDHNIV-FSZOTQKASA-N cycloheximide Chemical compound C1[C@@H](C)C[C@H](C)C(=O)[C@@H]1[C@H](O)CC1CC(=O)NC(=O)C1 YPHMISFOHDHNIV-FSZOTQKASA-N 0.000 description 1
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LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 description 1
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238000001914 filtration Methods 0.000 description 1
239000011888 foil Substances 0.000 description 1
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238000007710 freezing Methods 0.000 description 1
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238000003306 harvesting Methods 0.000 description 1
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238000006703 hydration reaction Methods 0.000 description 1
150000003840 hydrochlorides Chemical class 0.000 description 1
239000004615 ingredient Substances 0.000 description 1
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238000011081 inoculation Methods 0.000 description 1
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150000002500 ions Chemical class 0.000 description 1
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239000011325 microbead Substances 0.000 description 1
210000002200 mouth mucosa Anatomy 0.000 description 1
BEPINZGLNNOOIJ-UHFFFAOYSA-N n-(bromomethyl)-n-ethylethanamine Chemical compound CCN(CC)CBr BEPINZGLNNOOIJ-UHFFFAOYSA-N 0.000 description 1
ZBUQPAJRQXISTC-UHFFFAOYSA-N n-(chloromethyl)-n-ethylethanamine Chemical compound CCN(CC)CCl ZBUQPAJRQXISTC-UHFFFAOYSA-N 0.000 description 1
229910052760 oxygen Inorganic materials 0.000 description 1
239000001301 oxygen Substances 0.000 description 1
239000000825 pharmaceutical preparation Substances 0.000 description 1
229940127557 pharmaceutical product Drugs 0.000 description 1
230000007505 plaque formation Effects 0.000 description 1
229920000447 polyanionic polymer Polymers 0.000 description 1
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239000002244 precipitate Substances 0.000 description 1
230000001376 precipitating effect Effects 0.000 description 1
230000001566 pro-viral effect Effects 0.000 description 1
230000001737 promoting effect Effects 0.000 description 1
102000004169 proteins and genes Human genes 0.000 description 1
108090000623 proteins and genes Proteins 0.000 description 1
230000002685 pulmonary effect Effects 0.000 description 1
239000002994 raw material Substances 0.000 description 1
230000002829 reductive effect Effects 0.000 description 1
230000004044 response Effects 0.000 description 1
230000000717 retained effect Effects 0.000 description 1
229920006395 saturated elastomer Polymers 0.000 description 1
229910001961 silver nitrate Inorganic materials 0.000 description 1
239000002002 slurry Substances 0.000 description 1
238000001179 sorption measurement Methods 0.000 description 1
239000012086 standard solution Substances 0.000 description 1
239000008107 starch Substances 0.000 description 1
235000019698 starch Nutrition 0.000 description 1
238000003756 stirring Methods 0.000 description 1
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SZWHXXNVLACKBV-UHFFFAOYSA-N tetraethylphosphanium Chemical group CC[P+](CC)(CC)CC SZWHXXNVLACKBV-UHFFFAOYSA-N 0.000 description 1
238000004448 titration Methods 0.000 description 1
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239000012588 trypsin Substances 0.000 description 1
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Classifications

- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/52—Cytokines; Lymphokines; Interferons
- C07K14/555—Interferons [IFN]
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/52—Cytokines; Lymphokines; Interferons
- C07K14/555—Interferons [IFN]
- C07K14/565—IFN-beta
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/0068—General culture methods using substrates
- C12N5/0075—General culture methods using substrates using microcarriers
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2531/00—Microcarriers
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2533/00—Supports or coatings for cell culture, characterised by material
- C12N2533/70—Polysaccharides

Landscapes

Health & Medical Sciences (AREA)
Chemical & Material Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Organic Chemistry (AREA)
Zoology (AREA)
Genetics & Genomics (AREA)
Engineering & Computer Science (AREA)
General Health & Medical Sciences (AREA)
Biochemistry (AREA)
Bioinformatics & Cheminformatics (AREA)
Biophysics (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Molecular Biology (AREA)
Medicinal Chemistry (AREA)
Biotechnology (AREA)
Biomedical Technology (AREA)
Toxicology (AREA)
Gastroenterology & Hepatology (AREA)
Wood Science & Technology (AREA)
General Engineering & Computer Science (AREA)
Cell Biology (AREA)
Microbiology (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)
Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

CH1382677A 1976-11-11 1977-11-11 Process for the cultivation of anchorage-dependent cells in a microcarrier culture, microcarriers for carrying it out and use of the process. CH615947A5 (en)

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
US74099376A	1976-11-11	1976-11-11

Publications (1)

Publication Number	Publication Date
CH615947A5 true CH615947A5 (en)	1980-02-29

Family

ID=24978920

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
CH1382677A CH615947A5 (en)	1976-11-11	1977-11-11	Process for the cultivation of anchorage-dependent cells in a microcarrier culture, microcarriers for carrying it out and use of the process.

Country Status (14)

Country	Link
JP (3)	JPS5362889A (fi)
AU (1)	AU508398B2 (fi)
BE (1)	BE860721A (fi)
BR (1)	BR7707551A (fi)
CA (1)	CA1063050A (fi)
CH (1)	CH615947A5 (fi)
DE (2)	DE2759579C2 (fi)
FI (1)	FI60407C (fi)
FR (1)	FR2370792A1 (fi)
GB (1)	GB1535150A (fi)
IN (1)	IN147612B (fi)
NL (1)	NL177926C (fi)
SE (2)	SE417108C (fi)
ZA (1)	ZA776251B (fi)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
ZA776251B (en) *	1976-11-11	1978-07-26	Massachusetts Inst Technology	Improved cell culture microcarriers
FR2470794A1 (fr) *	1979-12-05	1981-06-12	Pasteur Institut	Nouvelles microparticules, leur preparation et leurs applications en biologie, notamment a la culture de cellules diploides humaines
US4448884A (en) *	1982-03-03	1984-05-15	Kms Fusion, Inc.	Glass-surface microcarrier for growth of cell cultures
JPS592735U (ja) *	1982-06-28	1984-01-09	シャープ株式会社	給紙用カセツト内の用紙残量指示装置
JPS60126453U (ja) *	1984-02-02	1985-08-26	富士ゼロックス株式会社	フアクシミリ等の用紙残量表示装置
IL74259A0 (en) *	1984-02-06	1985-05-31	Surface Concepts Pty Ltd	Improved method for cell culture
JPS60145139U (ja) *	1984-03-07	1985-09-26	日本電信電話株式会社	シ−ト状記録紙の残量表示装置
SE454518B (sv) *	1984-05-21	1988-05-09	Statens Bakteriologiska Lab	Forfarande for odling av diploida celler i nervaro av cellulosafibrer
JP2606213B2 (ja) *	1986-04-22	1997-04-30	味の素株式会社	修飾された微生物産生セルロースのゲルおよび動物細胞膜との複合体
DE3633891A1 (de) *	1986-10-04	1988-04-07	Akzo Gmbh	Verfahren und vorrichtung zum kultivieren von tierischen zellen
US20140170748A1 (en) *	2012-12-14	2014-06-19	DePuy Synthes Products, LLC	Nutrient Enriched Media for hUTC Growth
CN113249311B (zh) *	2020-05-25	2022-06-28	北京唐颐惠康生物医学技术有限公司	间充质干细胞培养用可降解微载体的制备方法及其产品和应用

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
GB1436323A (en) *	1972-04-26	1976-05-19	Wellcome Found	Cell and virus culture systems
US3901095A (en) *	1974-06-17	1975-08-26	Joseph W Wechsler	Bicycle gear shift
US3935067A (en) *	1974-11-22	1976-01-27	Wyo-Ben Products, Inc.	Inorganic support for culture media
ZA776251B (en) *	1976-11-11	1978-07-26	Massachusetts Inst Technology	Improved cell culture microcarriers
JPS5614270A (en) *	1979-07-14	1981-02-12	Ricoh Co Ltd	Wet type development after treating device

1977
- 1977-10-20 ZA ZA00776251A patent/ZA776251B/xx unknown
- 1977-10-27 GB GB44659/77A patent/GB1535150A/en not_active Expired
- 1977-10-31 IN IN1561/CAL/77A patent/IN147612B/en unknown
- 1977-11-04 NL NLAANVRAGE7712202,A patent/NL177926C/xx not_active IP Right Cessation
- 1977-11-08 DE DE2759579A patent/DE2759579C2/de not_active Expired
- 1977-11-08 DE DE2749989A patent/DE2749989C3/de not_active Expired
- 1977-11-09 JP JP13365277A patent/JPS5362889A/ja active Granted
- 1977-11-09 AU AU30491/77A patent/AU508398B2/en not_active Expired
- 1977-11-10 SE SE7712700A patent/SE417108C/xx not_active IP Right Cessation
- 1977-11-10 FR FR7733954A patent/FR2370792A1/fr active Granted
- 1977-11-10 CA CA290,641A patent/CA1063050A/en not_active Expired
- 1977-11-10 BE BE182543A patent/BE860721A/xx not_active IP Right Cessation
- 1977-11-10 BR BR7707551A patent/BR7707551A/pt unknown
- 1977-11-10 FI FI773377A patent/FI60407C/fi not_active IP Right Cessation
- 1977-11-11 CH CH1382677A patent/CH615947A5/de not_active IP Right Cessation
1980
- 1980-10-07 SE SE8006993A patent/SE460906B/sv not_active IP Right Cessation
- 1980-10-15 JP JP14308980A patent/JPS5699789A/ja active Granted
- 1980-10-15 JP JP14309080A patent/JPS5699790A/ja active Granted

Also Published As

Publication number	Publication date
IN147612B (fi)	1980-05-03
JPS5735953B2 (fi)	1982-07-31
DE2749989C3 (de)	1980-07-03
CA1063050A (en)	1979-09-25
AU3049177A (en)	1979-06-28
FI60407B (fi)	1981-09-30
DE2749989A1 (de)	1978-05-18
DE2749989B2 (de)	1979-10-25
FR2370792A1 (fr)	1978-06-09
GB1535150A (en)	1978-12-06
SE460906B (sv)	1989-12-04
SE417108C (sv)	1989-04-17
BR7707551A (pt)	1978-06-20
BE860721A (nl)	1978-03-01
SE8006993L (sv)	1980-10-07
JPS5699789A (en)	1981-08-11
DE2759579C2 (de)	1983-08-25
SE7712700L (sv)	1978-05-12
JPS575514B2 (fi)	1982-01-30
FI60407C (fi)	1982-01-11
JPS5614270B2 (fi)	1981-04-03
FI773377A (fi)	1978-05-12
NL7712202A (nl)	1978-05-16
AU508398B2 (en)	1980-03-20
NL177926B (nl)	1985-07-16
ZA776251B (en)	1978-07-26
JPS5699790A (en)	1981-08-11
FR2370792B1 (fi)	1983-07-29
JPS5362889A (en)	1978-06-05
NL177926C (nl)	1985-12-16
SE417108B (sv)	1981-02-23

Publication	Publication Date	Title
DE3033885C2 (fi)	1988-04-07
US4189534A (en)	1980-02-19	Cell culture microcarriers
US4293654A (en)	1981-10-06	Cell culture microcarriers
DE69231485T2 (de)	2001-05-10	Verwendung von porösem Calciumcarbonat als Trägermaterial für in vitro Zellkulturen.
DE2940150A1 (de)	1980-08-21	Mikrotraeger-zellkultur
CH684892A5 (de)	1995-01-31	DNA, welche für ein Polypeptid mit der Aktivität von menschlichem Interferon und beta 2 kodiert und Verfahren zu dessen Herstellung.
CH615947A5 (en)	1980-02-29	Process for the cultivation of anchorage-dependent cells in a microcarrier culture, microcarriers for carrying it out and use of the process.
DE3509202A1 (de)	1986-04-24	Verfahren zur induktion einer immunantwort durch immunisierung mit eingekapselten antigen erzeugenden zellen
WO2009010232A1 (de)	2009-01-22	Biomaterial basierend auf einem hydrophilen polymeren träger
DE3875396T2 (de)	1993-03-04	Mikrotraegermaterial fuer die zellkultur.
EP0005476A2 (de)	1979-11-28	Verfahren zur Herstellung von Humaninterferon
DE10307925A1 (de)	2003-09-04	Träger für eine Zellkultur und Verfahren zur Kultivierung von Zellen
DE3402927C2 (fi)	1989-03-09
DE2828073C2 (de)	1983-05-05	Verfahren zum Kultivieren von Viren
DE3434122A1 (de)	1985-06-05	Verfahren zur herstellung von interferon
DE3752184T2 (de)	1998-10-29	Herstellung und Verwendungen von gamma-Interferon
DE3325131C2 (fi)	1987-08-13
DE3786698T2 (de)	1993-12-23	Immobilisierung von nichtverankerungsabhängigen Zellen.
DE4123660C2 (fi)	1993-07-29
DE3226320A1 (de)	1983-02-10	Plasminogenaktivator und verfahren zu seiner herstellung
CH646875A5 (de)	1984-12-28	Verfahren zur gewinnung eines interferons und interferon hergestellt nach diesem verfahren.
WO1996007730A2 (de)	1996-03-14	Chemisches verfahren zur förderung der proliferation von tierischen zellen
EP0291696A2 (de)	1988-11-23	Verfahren zur Vermehrung von Epithelialzellen
DE2821466A1 (de)	1979-11-29	Verbessertes verfahren zur herstellung von humaninterferon
EP1490112A1 (de)	2004-12-29	Verfahren und mittel zur herstellung therapeutisch wirksamer blutzusammensetzungen

Legal Events

Date	Code	Title	Description
1997-11-28	PL	Patent ceased