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CA3177590A1 - Procede pour l'isolement de proteines fonctionnelles solubles a partir de matiere vegetale - Google Patents

Procede pour l'isolement de proteines fonctionnelles solubles a partir de matiere vegetale Download PDF

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Publication number
CA3177590A1
CA3177590A1 CA3177590A CA3177590A CA3177590A1 CA 3177590 A1 CA3177590 A1 CA 3177590A1 CA 3177590 A CA3177590 A CA 3177590A CA 3177590 A CA3177590 A CA 3177590A CA 3177590 A1 CA3177590 A1 CA 3177590A1
Authority
CA
Canada
Prior art keywords
permeate
retentate
centrifugation
stream
resulting
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CA3177590A
Other languages
English (en)
Inventor
Andre Banier De Haan
Marlies Elisabeth Johanna GEERTS
Paulus Statius Reinier Kosters
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Koninklijke Cooperatie Cosun UA
Original Assignee
Koninklijke Cooperatie Cosun UA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Koninklijke Cooperatie Cosun UA filed Critical Koninklijke Cooperatie Cosun UA
Publication of CA3177590A1 publication Critical patent/CA3177590A1/fr
Pending legal-status Critical Current

Links

Classifications

    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23JPROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
    • A23J1/00Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
    • A23J1/006Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from vegetable materials
    • A23J1/007Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from vegetable materials from leafy vegetables, e.g. alfalfa, clover, grass
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K1/00General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14Extraction; Separation; Purification
    • C07K1/36Extraction; Separation; Purification by a combination of two or more processes of different types
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23JPROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
    • A23J1/00Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
    • A23J1/001Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from waste materials, e.g. kitchen waste
    • A23J1/005Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from waste materials, e.g. kitchen waste from vegetable waste materials
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01DSEPARATION
    • B01D15/00Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08Selective adsorption, e.g. chromatography
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01DSEPARATION
    • B01D21/00Separation of suspended solid particles from liquids by sedimentation
    • B01D21/01Separation of suspended solid particles from liquids by sedimentation using flocculating agents
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01DSEPARATION
    • B01D21/00Separation of suspended solid particles from liquids by sedimentation
    • B01D21/26Separation of sediment aided by centrifugal force or centripetal force
    • B01D21/262Separation of sediment aided by centrifugal force or centripetal force by using a centrifuge
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01DSEPARATION
    • B01D25/00Filters formed by clamping together several filtering elements or parts of such elements
    • B01D25/12Filter presses, i.e. of the plate or plate and frame type
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01DSEPARATION
    • B01D61/00Processes of separation using semi-permeable membranes, e.g. dialysis, osmosis or ultrafiltration; Apparatus, accessories or auxiliary operations specially adapted therefor
    • B01D61/14Ultrafiltration; Microfiltration
    • B01D61/145Ultrafiltration
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01DSEPARATION
    • B01D61/00Processes of separation using semi-permeable membranes, e.g. dialysis, osmosis or ultrafiltration; Apparatus, accessories or auxiliary operations specially adapted therefor
    • B01D61/14Ultrafiltration; Microfiltration
    • B01D61/147Microfiltration
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01DSEPARATION
    • B01D61/00Processes of separation using semi-permeable membranes, e.g. dialysis, osmosis or ultrafiltration; Apparatus, accessories or auxiliary operations specially adapted therefor
    • B01D61/14Ultrafiltration; Microfiltration
    • B01D61/149Multistep processes comprising different kinds of membrane processes selected from ultrafiltration or microfiltration
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B04CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
    • B04BCENTRIFUGES
    • B04B1/00Centrifuges with rotary bowls provided with solid jackets for separating predominantly liquid mixtures with or without solid particles
    • B04B1/04Centrifuges with rotary bowls provided with solid jackets for separating predominantly liquid mixtures with or without solid particles with inserted separating walls
    • B04B1/08Centrifuges with rotary bowls provided with solid jackets for separating predominantly liquid mixtures with or without solid particles with inserted separating walls of conical shape
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B04CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
    • B04BCENTRIFUGES
    • B04B11/00Feeding, charging, or discharging bowls
    • B04B11/02Continuous feeding or discharging; Control arrangements therefor
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01DSEPARATION
    • B01D2315/00Details relating to the membrane module operation
    • B01D2315/16Diafiltration

Landscapes

  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Water Supply & Treatment (AREA)
  • Biochemistry (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Polymers & Plastics (AREA)
  • Food Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Botany (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Peptides Or Proteins (AREA)

Abstract

La présente invention concerne un procédé pour l'obtention de protéines fonctionnelles solubles à partir de matière végétale, comprenant les étapes consistant à : a) rompre mécaniquement les cellules de la matière végétale pour obtenir un courant de bouillie Ma ; b) soumettre le courant de bouillie Ma à une étape de séparation physique grossière, ce qui conduit à un perméat Pb et un rétentat Rb ; c) soumettre le perméat Pb à un traitement doux à une température comprise entre 20 °C et 60 °C pendant au moins 1 minute, ce qui conduit à un perméat traité Pc ; d) soumettre le perméat traité Pc à n étapes de centrifugation en série, n étant un nombre entier dans la plage de 2 à 5, chaque étape de centrifugation i, i étant un nombre entier compris entre 1 et n, conduisant à une fraction de culot X i et un centrat C i , le centrat C x de l'étape de centrifugation x, x étant un nombre entier dans la plage de 1 à n-1, étant soumis à une centrifugation dans l'étape de centrifugation x+1 ; e) soumettre le centrat C n à une étape de microfiltration conduisant à un perméat Pe et un rétentat Re; f) soumettre le perméat Pe à une étape d'ultrafiltration conduisant à un perméat Pf et un rétentat Rf; g) soumettre le rétentat Rf à une adsorption sur colonne hydrophobe pour fournir un perméat de colonne Pg et un rétentat Rg ; et h) faire sécher le perméat de colonne Pg pour obtenir un isolat de protéines fonctionnelles solubles, le procédé comprenant en outre une étape de recyclage.
CA3177590A 2020-05-18 2021-05-17 Procede pour l'isolement de proteines fonctionnelles solubles a partir de matiere vegetale Pending CA3177590A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
NL2025601A NL2025601B1 (en) 2020-05-18 2020-05-18 Process for isolating soluble functional proteins from plant material
NL2025601 2020-05-18
PCT/NL2021/050315 WO2021235930A1 (fr) 2020-05-18 2021-05-17 Procédé pour l'isolement de protéines fonctionnelles solubles à partir de matière végétale

Publications (1)

Publication Number Publication Date
CA3177590A1 true CA3177590A1 (fr) 2021-11-25

Family

ID=71575760

Family Applications (1)

Application Number Title Priority Date Filing Date
CA3177590A Pending CA3177590A1 (fr) 2020-05-18 2021-05-17 Procede pour l'isolement de proteines fonctionnelles solubles a partir de matiere vegetale

Country Status (5)

Country Link
US (1) US20230212219A1 (fr)
EP (1) EP4152941A1 (fr)
CA (1) CA3177590A1 (fr)
NL (1) NL2025601B1 (fr)
WO (1) WO2021235930A1 (fr)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK181537B1 (en) * 2022-09-23 2024-04-10 Permeco Aps A method for recovering proteins from a plant material
WO2024136679A1 (fr) * 2022-12-22 2024-06-27 Leaft Foods Ip Limited Appareil et procédé d'extraction

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3959246A (en) * 1974-07-08 1976-05-25 The United States Of America As Represented By The Secretary Of Agriculture Preparation of soluble edible protein from leafy green crops
US4268632A (en) 1979-09-24 1981-05-19 Leaf Proteins, Inc. Process for isolation of ribulose 1,5-diphosphate carboxylase from plant leaves
US4359530A (en) * 1980-09-08 1982-11-16 Aluminum Company Of America Protein extraction from green crops
GB2110221B (en) 1981-05-18 1984-10-03 Leaf Protein Inc Process for isolation of proteins from plant leaves
US9155323B2 (en) * 2009-05-15 2015-10-13 Siebte Pmi Verwaltungs Gmbh Aqueous process for preparing protein isolate and hydrolyzed protein from an oilseed
WO2011078671A1 (fr) 2009-12-22 2011-06-30 Nizo Food Research B.V. Procédé d'isolement d'une préparation de rubisco déchlorophyllée à partir d'une substance végétale
DK2934187T3 (da) 2012-12-24 2019-10-14 Stichting Wageningen Res Økonomisk fremgangsmåde til isolering af funktionelt protein fra planter
FR3054141B1 (fr) * 2016-07-22 2018-07-13 Institut National De La Recherche Agronomique Separation des enzymes issues de trichoderma reesei par filtre presse et filtration tangentielle sur membrane ceramique.
DE102016013059A1 (de) 2016-11-03 2018-05-03 Thomas Bruckner Schneckenfilterpresse zur Filtrierung und/oder Aufkonzentrierung eines fluiden, feststoffbeladenen Suspensionsstroms und Verfahren zur Filtrierung und/oder Aufkonzentrierung
WO2019215153A1 (fr) * 2018-05-07 2019-11-14 LIHME PROTEIN SOLUTIONS ApS Procédés intégrés de précipitation et de filtration membranaire pour l'isolement de protéines de pomme de terre

Also Published As

Publication number Publication date
US20230212219A1 (en) 2023-07-06
NL2025601B1 (en) 2021-12-03
WO2021235930A1 (fr) 2021-11-25
EP4152941A1 (fr) 2023-03-29

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