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CA3167640A1 - Populations de cellules dotees d'editions concues de maniere rationnelle - Google Patents

Populations de cellules dotees d'editions concues de maniere rationnelle

Info

Publication number
CA3167640A1
CA3167640A1 CA3167640A CA3167640A CA3167640A1 CA 3167640 A1 CA3167640 A1 CA 3167640A1 CA 3167640 A CA3167640 A CA 3167640A CA 3167640 A CA3167640 A CA 3167640A CA 3167640 A1 CA3167640 A1 CA 3167640A1
Authority
CA
Canada
Prior art keywords
editing
cells
module
cell
nickase
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CA3167640A
Other languages
English (en)
Inventor
Aamir MIR
Erik Zimmerman
Emily Feldman
Benjamin Mijts
Andrew GARST
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Inscripta Inc
Original Assignee
Inscripta Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US16/740,420 external-priority patent/US20210214671A1/en
Application filed by Inscripta Inc filed Critical Inscripta Inc
Publication of CA3167640A1 publication Critical patent/CA3167640A1/fr
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/48—Automatic or computerized control
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Mycology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Magnetic Record Carriers (AREA)

Abstract

La présente invention concerne des procédés et des instruments multi-modules automatisés pour augmenter le pourcentage de cellules de mammifères éditées dans une population de cellules lorsqu'on utilise l'édition guidée par acide nucléique.
CA3167640A 2020-01-11 2021-01-10 Populations de cellules dotees d'editions concues de maniere rationnelle Pending CA3167640A1 (fr)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US202016740421A 2020-01-11 2020-01-11
US16/740,420 2020-01-11
US16/740,420 US20210214671A1 (en) 2020-01-11 2020-01-11 Cell populations with rationally designed edits
US16/740,421 2020-01-11
PCT/US2021/012867 WO2021142394A1 (fr) 2020-01-11 2021-01-10 Populations de cellules dotées d'éditions conçues de manière rationnelle

Publications (1)

Publication Number Publication Date
CA3167640A1 true CA3167640A1 (fr) 2021-07-15

Family

ID=76787550

Family Applications (1)

Application Number Title Priority Date Filing Date
CA3167640A Pending CA3167640A1 (fr) 2020-01-11 2021-01-10 Populations de cellules dotees d'editions concues de maniere rationnelle

Country Status (5)

Country Link
EP (1) EP4087923A1 (fr)
CN (1) CN115279899A (fr)
AU (1) AU2021205422A1 (fr)
CA (1) CA3167640A1 (fr)
WO (1) WO2021142394A1 (fr)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2023102481A1 (fr) * 2021-12-02 2023-06-08 Inscripta, Inc. Édition guidée par un acide nucléique détectable
WO2023122023A1 (fr) * 2021-12-20 2023-06-29 Inscripta, Inc. Code-barres génomique ciblé pour le suivi des événements d'édition

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010093966A2 (fr) * 2009-02-12 2010-08-19 Fred Hutchinson Cancer Research Center Génération d'une enzyme de césure de l'adn stimulant la conversion d'un gène spécifique d'un site à partir d'une endonucléase de ciblage
EP3207139A1 (fr) * 2014-10-17 2017-08-23 The Penn State Research Foundation Procédés et compositions pour l'édition génomique multiplex à guidage arn et autres technologies d'arn
US10017760B2 (en) * 2016-06-24 2018-07-10 Inscripta, Inc. Methods for generating barcoded combinatorial libraries
RS63202B1 (sr) * 2017-06-30 2022-06-30 Inscripta Inc Automatizovani postupci, moduli, instrumenti i sistemi za obradu ćelija

Also Published As

Publication number Publication date
EP4087923A1 (fr) 2022-11-16
WO2021142394A1 (fr) 2021-07-15
AU2021205422A1 (en) 2022-09-08
CN115279899A (zh) 2022-11-01

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