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CA3084907A1 - Variants de cellulase fongique - Google Patents

Variants de cellulase fongique Download PDF

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Publication number
CA3084907A1
CA3084907A1 CA3084907A CA3084907A CA3084907A1 CA 3084907 A1 CA3084907 A1 CA 3084907A1 CA 3084907 A CA3084907 A CA 3084907A CA 3084907 A CA3084907 A CA 3084907A CA 3084907 A1 CA3084907 A1 CA 3084907A1
Authority
CA
Canada
Prior art keywords
cellulase
del
variant polypeptide
detergent
enzyme
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CA3084907A
Other languages
English (en)
Inventor
Kari Juntunen
Leena Valtakari
Susanna Makinen
Marika Alapuranen
Hendrik Hellmuth
Pentti Ojapalo
Marc Mennicken
Ulrich Schwaneberg
David Schonauer
Terhi Puranen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
AB Enzymes Oy
Original Assignee
AB Enzymes Oy
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from EP17209301.5A external-priority patent/EP3502243B1/fr
Priority claimed from US15/850,712 external-priority patent/US10570383B2/en
Application filed by AB Enzymes Oy filed Critical AB Enzymes Oy
Publication of CA3084907A1 publication Critical patent/CA3084907A1/fr
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C11D3/38645—Preparations containing enzymes, e.g. protease or amylase containing cellulase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01004—Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase
    • D—TEXTILES; PAPER
    • D06—TREATMENT OF TEXTILES OR THE LIKE; LAUNDERING; FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR
    • D06M—TREATMENT, NOT PROVIDED FOR ELSEWHERE IN CLASS D06, OF FIBRES, THREADS, YARNS, FABRICS, FEATHERS OR FIBROUS GOODS MADE FROM SUCH MATERIALS
    • D06M16/00—Biochemical treatment of fibres, threads, yarns, fabrics, or fibrous goods made from such materials, e.g. enzymatic
    • D06M16/003—Biochemical treatment of fibres, threads, yarns, fabrics, or fibrous goods made from such materials, e.g. enzymatic with enzymes or microorganisms
    • D—TEXTILES; PAPER
    • D06—TREATMENT OF TEXTILES OR THE LIKE; LAUNDERING; FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR
    • D06M—TREATMENT, NOT PROVIDED FOR ELSEWHERE IN CLASS D06, OF FIBRES, THREADS, YARNS, FABRICS, FEATHERS OR FIBROUS GOODS MADE FROM SUCH MATERIALS
    • D06M2200/00—Functionality of the treatment composition and/or properties imparted to the textile material
    • D06M2200/50—Modified hand or grip properties; Softening compositions

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Zoology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Medicinal Chemistry (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Textile Engineering (AREA)
  • Molecular Biology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Detergent Compositions (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

La présente invention concerne des polypeptides présentant une activité cellulase, en particulier des variants dérivés de l'enzyme 20K-cellulase. L'invention décrit un certain nombre de positions de résidus d'acides aminés, importantes pour les propriétés de l'enzyme cellulase et ainsi pour sa stabilité et/ou ses performances. Les nouveaux variants présentent une stabilité améliorée par comparaison avec la cellulase parentale. En particulier, les nouveaux variants présentent de bonnes performances dans une application anti-grisonnement et une excellente stabilité en présence d'une protéase dans plusieurs compositions détergentes, même dans des expériences sur le long terme.
CA3084907A 2017-12-21 2018-12-17 Variants de cellulase fongique Pending CA3084907A1 (fr)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
EP17209301.5A EP3502243B1 (fr) 2017-12-21 2017-12-21 Variantes de cellulase fongique
US15/850,712 US10570383B2 (en) 2017-12-21 2017-12-21 Variants of fungal cellulase
EP17209301.5 2017-12-21
US15/850,712 2017-12-21
PCT/FI2018/050935 WO2019122520A1 (fr) 2017-12-21 2018-12-17 Variants de cellulase fongique

Publications (1)

Publication Number Publication Date
CA3084907A1 true CA3084907A1 (fr) 2019-06-27

Family

ID=66992532

Family Applications (1)

Application Number Title Priority Date Filing Date
CA3084907A Pending CA3084907A1 (fr) 2017-12-21 2018-12-17 Variants de cellulase fongique

Country Status (6)

Country Link
EP (1) EP3728579A4 (fr)
KR (1) KR102413168B1 (fr)
CN (1) CN111670250B (fr)
BR (1) BR112020011352A2 (fr)
CA (1) CA3084907A1 (fr)
WO (1) WO2019122520A1 (fr)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109234174B (zh) * 2018-11-12 2021-04-27 陕西科技大学 一种产纤维素酶的嗜热真菌及其应用
DE102022205594A1 (de) 2022-06-01 2023-12-07 Henkel Ag & Co. Kgaa Leistungsverbesserte und lagerstabile protease-varianten
DE102022205593A1 (de) 2022-06-01 2023-12-07 Henkel Ag & Co. Kgaa Wasch- und reinigungsmittel mit verbesserter enzymstabilität
DE102022205591A1 (de) 2022-06-01 2023-12-07 Henkel Ag & Co. Kgaa Wasch- und reinigungsmittel mit verbesserter enzymstabilität
DE102022205588A1 (de) 2022-06-01 2023-12-07 Henkel Ag & Co. Kgaa Wasch- und reinigungsmittel mit verbesserter enzymstabilität
EP4324900A1 (fr) 2022-08-17 2024-02-21 Henkel AG & Co. KGaA Composition détergente comprenant des enzymes
DE102022211482A1 (de) 2022-10-28 2024-05-08 Henkel Ag & Co. Kgaa Naturfarbstoff-Färbung mit verbesserter Farbintensität
DE102022211856A1 (de) 2022-11-09 2024-05-16 Henkel Ag & Co. Kgaa Zweistufiges Färbeverfahren mit Naturfarbstoffen mit verbesserter Farbintensität
DE102022131732A1 (de) 2022-11-30 2024-06-06 Henkel Ag & Co. Kgaa Verbesserte Waschleistung durch den Einsatz einer Protease fusioniert mit speziellem Adhäsionsvermittlerpeptid
DE102023200106A1 (de) 2023-01-10 2024-07-11 Henkel Ag & Co. Kgaa Enzymhaltiges wasch- und reinigungsmittel
DE102023201692A1 (de) 2023-02-24 2024-08-29 Henkel Ag & Co. Kgaa Wasch- und reinigungsmittel mit dispersin und duftstoff
DE102023201695A1 (de) 2023-02-24 2024-08-29 Henkel Ag & Co. Kgaa Wasch- und reinigungsmittel mit dispersin
DE102023201696A1 (de) 2023-02-24 2024-08-29 Henkel Ag & Co. Kgaa Wasch- und reinigungsmittel mit dispersin
DE102023205632A1 (de) 2023-06-15 2024-12-19 Henkel Ag & Co. Kgaa Peptide mit schmutzablösender wirkung für wasch- und reinigungsmittel
CN117384891B (zh) * 2023-12-13 2024-03-15 北京科为博生物科技有限公司 一种热稳定性提高的酸性木聚糖酶及其基因与应用

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6723549B2 (en) * 1995-10-17 2004-04-20 Ab Enzymes Oy Cellulases, the genes encoding them and uses thereof
EP0857216B1 (fr) * 1995-10-17 2014-09-10 AB Enzymes Oy Cellulases, genes les codant et utilisation de ces cellulases
CN1244701C (zh) * 1995-10-17 2006-03-08 生化酶股份有限公司 纤维素酶,其编码基因及其应用
CN101085985B (zh) * 1996-09-17 2012-05-16 诺沃奇梅兹有限公司 纤维素酶变体
EP1735438A2 (fr) * 2004-04-12 2006-12-27 Catalyst Biosciences Clivage de vegf et de recepteur vegf par des proteases de types sauvage et mutantes
FI118340B (fi) * 2005-04-29 2007-10-15 Ab Enzymes Oy Sellulaasifuusioproteiineja, niiden tuotto ja käyttö
MX2007013474A (es) * 2005-04-29 2008-04-02 Ab Enzymes Oy Celulasas mejoradas.
US7741093B2 (en) * 2005-04-29 2010-06-22 Ab Enzymes Oy Cellulases and their uses
US9410135B2 (en) * 2010-09-15 2016-08-09 The Regents Of The University Of California Thermophilic mutants of Trichoderma reesei endoglucanase I
KR20140018890A (ko) * 2011-02-09 2014-02-13 노보자임스 에이/에스 탈필링을 위한 셀룰라제 효소 혼합물 및 그것의 이용
FI127093B (en) * 2014-10-27 2017-11-15 Ab Enzymes Oy Fungal-derived endoglucanase variants, their production and use
BR112017028065A2 (pt) * 2015-06-26 2018-09-11 Novozymes As sistema de bioacabamento
CN108463552A (zh) * 2015-11-16 2018-08-28 诺维信公司 纤维素酶变体和对其编码的多核苷酸
BR112018012020A2 (pt) * 2015-12-18 2018-12-04 Danisco Us Inc polipeptídeos com atividade de endoglucanase e usos dos mesmos
EP3653706A1 (fr) * 2018-11-13 2020-05-20 AB Enzymes Oy Protéine de fusion

Also Published As

Publication number Publication date
EP3728579A4 (fr) 2021-12-01
CN111670250B (zh) 2024-02-13
WO2019122520A1 (fr) 2019-06-27
EP3728579A1 (fr) 2020-10-28
KR102413168B1 (ko) 2022-06-24
WO2019122520A8 (fr) 2020-10-01
CN111670250A (zh) 2020-09-15
KR20200105844A (ko) 2020-09-09
BR112020011352A2 (pt) 2020-11-17

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