CA2785699A1 - Polynucleotides codant des polypeptides de type c du vih antigeniques, polypeptides et leurs utilisations - Google Patents

Polynucleotides codant des polypeptides de type c du vih antigeniques, polypeptides et leurs utilisations Download PDF

Info

Publication number: CA2785699A1
Authority: CA; Canada
Prior art keywords: seq; vector; sequence; expression; expression cassette
Prior art date: 2001-07-05
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Abandoned

Application number

CA2785699A

Other languages

English (en)

Inventor

Jan Zur Megede

Susan W. Barnett

Ying Lian

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

Novartis Vaccines and Diagnostics Inc

Original Assignee

Novartis Vaccines and Diagnostics Inc

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

2001-07-05

Filing date

2002-07-05

Publication date

2003-01-16

2002-07-05 Application filed by Novartis Vaccines and Diagnostics Inc filed Critical Novartis Vaccines and Diagnostics Inc

2002-07-05 Priority claimed from CA2634992A external-priority patent/CA2634992C/fr

2003-01-16 Publication of CA2785699A1 publication Critical patent/CA2785699A1/fr

Status Abandoned legal-status Critical Current

Links

108090000765 processed proteins & peptides Proteins 0.000 title claims abstract description 247
102000004196 processed proteins & peptides Human genes 0.000 title claims abstract description 237
229920001184 polypeptide Polymers 0.000 title claims abstract description 236
102000040430 polynucleotide Human genes 0.000 title claims abstract description 150
108091033319 polynucleotide Proteins 0.000 title claims abstract description 150
239000002157 polynucleotide Substances 0.000 title claims abstract description 150
230000000890 antigenic effect Effects 0.000 title abstract description 16
230000003053 immunization Effects 0.000 claims abstract description 80
238000002649 immunization Methods 0.000 claims abstract description 75
238000004519 manufacturing process Methods 0.000 claims abstract description 40
230000014509 gene expression Effects 0.000 claims description 325
239000013598 vector Substances 0.000 claims description 166
238000000034 method Methods 0.000 claims description 89
239000002245 particle Substances 0.000 claims description 47
238000013519 translation Methods 0.000 claims description 44
230000028993 immune response Effects 0.000 claims description 38
239000013603 viral vector Substances 0.000 claims description 27
238000001476 gene delivery Methods 0.000 claims description 26
239000002502 liposome Substances 0.000 claims description 18
230000024932 T cell mediated immunity Effects 0.000 claims description 17
230000001177 retroviral effect Effects 0.000 claims description 17
230000035897 transcription Effects 0.000 claims description 15
238000013518 transcription Methods 0.000 claims description 15
230000028996 humoral immune response Effects 0.000 claims description 13
230000008488 polyadenylation Effects 0.000 claims description 12
230000000977 initiatory effect Effects 0.000 claims description 10
238000002360 preparation method Methods 0.000 claims description 10
229940065638 intron a Drugs 0.000 claims description 9
230000005030 transcription termination Effects 0.000 claims description 9
238000003259 recombinant expression Methods 0.000 claims description 8
108700028146 Genetic Enhancer Elements Proteins 0.000 claims description 7
238000005457 optimization Methods 0.000 claims description 7
108090000157 Metallothionein Proteins 0.000 claims description 4
PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 claims description 4
239000010931 gold Substances 0.000 claims description 4
229910052737 gold Inorganic materials 0.000 claims description 4
WFKWXMTUELFFGS-UHFFFAOYSA-N tungsten Chemical compound [W] WFKWXMTUELFFGS-UHFFFAOYSA-N 0.000 claims description 4
229910052721 tungsten Inorganic materials 0.000 claims description 4
239000010937 tungsten Substances 0.000 claims description 4
DIGQNXIGRZPYDK-WKSCXVIASA-N (2R)-6-amino-2-[[2-[[(2S)-2-[[2-[[(2R)-2-[[(2S)-2-[[(2R,3S)-2-[[2-[[(2S)-2-[[2-[[(2S)-2-[[(2S)-2-[[(2R)-2-[[(2S,3S)-2-[[(2R)-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-[[2-[[(2S)-2-[[(2R)-2-[[2-[[2-[[2-[(2-amino-1-hydroxyethylidene)amino]-3-carboxy-1-hydroxypropylidene]amino]-1-hydroxy-3-sulfanylpropylidene]amino]-1-hydroxyethylidene]amino]-1-hydroxy-3-sulfanylpropylidene]amino]-1,3-dihydroxypropylidene]amino]-1-hydroxyethylidene]amino]-1-hydroxypropylidene]amino]-1,3-dihydroxypropylidene]amino]-1,3-dihydroxypropylidene]amino]-1-hydroxy-3-sulfanylpropylidene]amino]-1,3-dihydroxybutylidene]amino]-1-hydroxy-3-sulfanylpropylidene]amino]-1-hydroxypropylidene]amino]-1,3-dihydroxypropylidene]amino]-1-hydroxyethylidene]amino]-1,5-dihydroxy-5-iminopentylidene]amino]-1-hydroxy-3-sulfanylpropylidene]amino]-1,3-dihydroxybutylidene]amino]-1-hydroxy-3-sulfanylpropylidene]amino]-1,3-dihydroxypropylidene]amino]-1-hydroxyethylidene]amino]-1-hydroxy-3-sulfanylpropylidene]amino]-1-hydroxyethylidene]amino]hexanoic acid Chemical compound C[C@@H]([C@@H](C(=N[C@@H](CS)C(=N[C@@H](C)C(=N[C@@H](CO)C(=NCC(=N[C@@H](CCC(=N)O)C(=NC(CS)C(=N[C@H]([C@H](C)O)C(=N[C@H](CS)C(=N[C@H](CO)C(=NCC(=N[C@H](CS)C(=NCC(=N[C@H](CCCCN)C(=O)O)O)O)O)O)O)O)O)O)O)O)O)O)O)N=C([C@H](CS)N=C([C@H](CO)N=C([C@H](CO)N=C([C@H](C)N=C(CN=C([C@H](CO)N=C([C@H](CS)N=C(CN=C(C(CS)N=C(C(CC(=O)O)N=C(CN)O)O)O)O)O)O)O)O)O)O)O)O DIGQNXIGRZPYDK-WKSCXVIASA-N 0.000 claims description 2
102000003792 Metallothionein Human genes 0.000 claims description 2
239000000203 mixture Substances 0.000 abstract description 88
238000004806 packaging method and process Methods 0.000 abstract description 39
230000002163 immunogen Effects 0.000 abstract description 16
238000009472 formulation Methods 0.000 abstract description 8
210000004027 cell Anatomy 0.000 description 268
108090000623 proteins and genes Proteins 0.000 description 247
241000725303 Human immunodeficiency virus Species 0.000 description 144
108020004414 DNA Proteins 0.000 description 138
108091007433 antigens Proteins 0.000 description 115
102000036639 antigens Human genes 0.000 description 115
102000004169 proteins and genes Human genes 0.000 description 114
239000000427 antigen Substances 0.000 description 112
235000018102 proteins Nutrition 0.000 description 104
101710149951 Protein Tat Proteins 0.000 description 95
150000007523 nucleic acids Chemical class 0.000 description 58
108091026890 Coding region Proteins 0.000 description 56
102100034343 Integrase Human genes 0.000 description 50
239000002773 nucleotide Substances 0.000 description 49
230000014616 translation Effects 0.000 description 49
125000003729 nucleotide group Chemical group 0.000 description 48
208000031886 HIV Infections Diseases 0.000 description 46
210000001744 T-lymphocyte Anatomy 0.000 description 46
241000700605 Viruses Species 0.000 description 46
102000039446 nucleic acids Human genes 0.000 description 46
108020004707 nucleic acids Proteins 0.000 description 46
239000004365 Protease Substances 0.000 description 43
230000035772 mutation Effects 0.000 description 43
108091005804 Peptidases Proteins 0.000 description 40
102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 40
241000713772 Human immunodeficiency virus 1 Species 0.000 description 39
235000001014 amino acid Nutrition 0.000 description 39
238000003556 assay Methods 0.000 description 37
229960005486 vaccine Drugs 0.000 description 36
229940024606 amino acid Drugs 0.000 description 34
108010092799 RNA-directed DNA polymerase Proteins 0.000 description 32
241001465754 Metazoa Species 0.000 description 30
210000004962 mammalian cell Anatomy 0.000 description 30
239000013612 plasmid Substances 0.000 description 30
238000003776 cleavage reaction Methods 0.000 description 29
239000000047 product Substances 0.000 description 29
230000007017 scission Effects 0.000 description 29
210000001151 cytotoxic T lymphocyte Anatomy 0.000 description 28
150000001413 amino acids Chemical class 0.000 description 27
125000003275 alpha amino acid group Chemical group 0.000 description 26
230000000694 effects Effects 0.000 description 25
239000012634 fragment Substances 0.000 description 25
238000012546 transfer Methods 0.000 description 25
230000003612 virological effect Effects 0.000 description 25
238000012217 deletion Methods 0.000 description 24
230000037430 deletion Effects 0.000 description 24
-1 RNAseH Proteins 0.000 description 23
239000002671 adjuvant Substances 0.000 description 23
230000027455 binding Effects 0.000 description 23
239000013604 expression vector Substances 0.000 description 23
108020004705 Codon Proteins 0.000 description 22
238000002965 ELISA Methods 0.000 description 22
241000699670 Mus sp. Species 0.000 description 21
230000003472 neutralizing effect Effects 0.000 description 21
108700010070 Codon Usage Proteins 0.000 description 20
210000002966 serum Anatomy 0.000 description 20
208000015181 infectious disease Diseases 0.000 description 19
241000283973 Oryctolagus cuniculus Species 0.000 description 18
230000004048 modification Effects 0.000 description 18
238000012986 modification Methods 0.000 description 18
230000004044 response Effects 0.000 description 18
230000000875 corresponding effect Effects 0.000 description 17
108090000695 Cytokines Proteins 0.000 description 16
108010061833 Integrases Proteins 0.000 description 16
108091028043 Nucleic acid sequence Proteins 0.000 description 16
101710090322 Truncated surface protein Proteins 0.000 description 16
241000700618 Vaccinia virus Species 0.000 description 16
238000001415 gene therapy Methods 0.000 description 16
238000001727 in vivo Methods 0.000 description 16
241001430294 unidentified retrovirus Species 0.000 description 16
102000004127 Cytokines Human genes 0.000 description 15
241000238631 Hexapoda Species 0.000 description 15
108020004684 Internal Ribosome Entry Sites Proteins 0.000 description 15
108010078428 env Gene Products Proteins 0.000 description 15
230000006870 function Effects 0.000 description 15
239000000463 material Substances 0.000 description 15
239000000523 sample Substances 0.000 description 14
108010000227 GP 140 Proteins 0.000 description 13
238000010790 dilution Methods 0.000 description 13
239000012895 dilution Substances 0.000 description 13
238000009396 hybridization Methods 0.000 description 13
238000006386 neutralization reaction Methods 0.000 description 13
238000001890 transfection Methods 0.000 description 13
238000013459 approach Methods 0.000 description 12
238000002474 experimental method Methods 0.000 description 12
230000001976 improved effect Effects 0.000 description 12
230000001965 increasing effect Effects 0.000 description 12
239000003550 marker Substances 0.000 description 12
108020004999 messenger RNA Proteins 0.000 description 12
230000010076 replication Effects 0.000 description 12
108010017213 Granulocyte-Macrophage Colony-Stimulating Factor Proteins 0.000 description 11
102100034353 Integrase Human genes 0.000 description 11
206010046865 Vaccinia virus infection Diseases 0.000 description 11
239000002253 acid Substances 0.000 description 11
239000000969 carrier Substances 0.000 description 11
238000011260 co-administration Methods 0.000 description 11
238000004520 electroporation Methods 0.000 description 11
230000005847 immunogenicity Effects 0.000 description 11
239000000126 substance Substances 0.000 description 11
239000006228 supernatant Substances 0.000 description 11
208000007089 vaccinia Diseases 0.000 description 11
101710177291 Gag polyprotein Proteins 0.000 description 10
102100039620 Granulocyte-macrophage colony-stimulating factor Human genes 0.000 description 10
240000004808 Saccharomyces cerevisiae Species 0.000 description 10
235000014680 Saccharomyces cerevisiae Nutrition 0.000 description 10
150000007513 acids Chemical class 0.000 description 10
230000005875 antibody response Effects 0.000 description 10
230000002238 attenuated effect Effects 0.000 description 10
210000003719 b-lymphocyte Anatomy 0.000 description 10
230000008901 benefit Effects 0.000 description 10
230000001413 cellular effect Effects 0.000 description 10
108010027225 gag-pol Fusion Proteins Proteins 0.000 description 10
238000002347 injection Methods 0.000 description 10
239000007924 injection Substances 0.000 description 10
241000701447 unidentified baculovirus Species 0.000 description 10
108010002350 Interleukin-2 Proteins 0.000 description 9
102000000588 Interleukin-2 Human genes 0.000 description 9
101710125418 Major capsid protein Proteins 0.000 description 9
108020004566 Transfer RNA Proteins 0.000 description 9
101800001690 Transmembrane protein gp41 Proteins 0.000 description 9
230000001580 bacterial effect Effects 0.000 description 9
238000006206 glycosylation reaction Methods 0.000 description 9
239000003102 growth factor Substances 0.000 description 9
238000000338 in vitro Methods 0.000 description 9
230000006698 induction Effects 0.000 description 9
210000004698 lymphocyte Anatomy 0.000 description 9
230000001404 mediated effect Effects 0.000 description 9
238000006467 substitution reaction Methods 0.000 description 9
208000030507 AIDS Diseases 0.000 description 8
241000699802 Cricetulus griseus Species 0.000 description 8
230000006820 DNA synthesis Effects 0.000 description 8
102000004889 Interleukin-6 Human genes 0.000 description 8
108090001005 Interleukin-6 Proteins 0.000 description 8
241000282560 Macaca mulatta Species 0.000 description 8
241000699666 Mus <mouse, genus> Species 0.000 description 8
206010028980 Neoplasm Diseases 0.000 description 8
230000004913 activation Effects 0.000 description 8
238000007792 addition Methods 0.000 description 8
239000002299 complementary DNA Substances 0.000 description 8
239000003623 enhancer Substances 0.000 description 8
230000013595 glycosylation Effects 0.000 description 8
230000002779 inactivation Effects 0.000 description 8
230000005764 inhibitory process Effects 0.000 description 8
229940100601 interleukin-6 Drugs 0.000 description 8
150000002632 lipids Chemical class 0.000 description 8
239000011859 microparticle Substances 0.000 description 8
230000025308 nuclear transport Effects 0.000 description 8
238000006384 oligomerization reaction Methods 0.000 description 8
239000013615 primer Substances 0.000 description 8
238000001262 western blot Methods 0.000 description 8
241000196324 Embryophyta Species 0.000 description 7
102100038195 Exonuclease mut-7 homolog Human genes 0.000 description 7
101000958030 Homo sapiens Exonuclease mut-7 homolog Proteins 0.000 description 7
102000000646 Interleukin-3 Human genes 0.000 description 7
108010002386 Interleukin-3 Proteins 0.000 description 7
241000713666 Lentivirus Species 0.000 description 7
108091034117 Oligonucleotide Proteins 0.000 description 7
108010076039 Polyproteins Proteins 0.000 description 7
239000011230 binding agent Substances 0.000 description 7
230000003197 catalytic effect Effects 0.000 description 7
210000004978 chinese hamster ovary cell Anatomy 0.000 description 7
238000003780 insertion Methods 0.000 description 7
230000037431 insertion Effects 0.000 description 7
229940076264 interleukin-3 Drugs 0.000 description 7
230000023603 positive regulation of transcription initiation, DNA-dependent Effects 0.000 description 7
230000003389 potentiating effect Effects 0.000 description 7
238000001742 protein purification Methods 0.000 description 7
238000000746 purification Methods 0.000 description 7
210000000130 stem cell Anatomy 0.000 description 7
238000010361 transduction Methods 0.000 description 7
230000026683 transduction Effects 0.000 description 7
241000710929 Alphavirus Species 0.000 description 6
241000271566 Aves Species 0.000 description 6
238000011238 DNA vaccination Methods 0.000 description 6
241000710188 Encephalomyocarditis virus Species 0.000 description 6
108010048209 Human Immunodeficiency Virus Proteins Proteins 0.000 description 6
108700018351 Major Histocompatibility Complex Proteins 0.000 description 6
102000003992 Peroxidases Human genes 0.000 description 6
101710137500 T7 RNA polymerase Proteins 0.000 description 6
241000251539 Vertebrata <Metazoa> Species 0.000 description 6
108010067390 Viral Proteins Proteins 0.000 description 6
JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 6
230000015572 biosynthetic process Effects 0.000 description 6
108010006025 bovine growth hormone Proteins 0.000 description 6
125000002091 cationic group Chemical group 0.000 description 6
239000002458 cell surface marker Substances 0.000 description 6
239000003153 chemical reaction reagent Substances 0.000 description 6
238000010276 construction Methods 0.000 description 6
210000002443 helper t lymphocyte Anatomy 0.000 description 6
210000000987 immune system Anatomy 0.000 description 6
230000001939 inductive effect Effects 0.000 description 6
210000002540 macrophage Anatomy 0.000 description 6
230000007498 myristoylation Effects 0.000 description 6
244000052769 pathogen Species 0.000 description 6
230000001717 pathogenic effect Effects 0.000 description 6
108040007629 peroxidase activity proteins Proteins 0.000 description 6
230000036515 potency Effects 0.000 description 6
230000020382 suppression by virus of host antigen processing and presentation of peptide antigen via MHC class I Effects 0.000 description 6
230000010474 transient expression Effects 0.000 description 6
241000701161 unidentified adenovirus Species 0.000 description 6
241000894006 Bacteria Species 0.000 description 5
241000700198 Cavia Species 0.000 description 5
102100024746 Dihydrofolate reductase Human genes 0.000 description 5
102000004190 Enzymes Human genes 0.000 description 5
108090000790 Enzymes Proteins 0.000 description 5
241000713340 Human immunodeficiency virus 2 Species 0.000 description 5
102000004388 Interleukin-4 Human genes 0.000 description 5
108090000978 Interleukin-4 Proteins 0.000 description 5
108010002616 Interleukin-5 Proteins 0.000 description 5
102100039897 Interleukin-5 Human genes 0.000 description 5
102000007651 Macrophage Colony-Stimulating Factor Human genes 0.000 description 5
108010046938 Macrophage Colony-Stimulating Factor Proteins 0.000 description 5
241000282520 Papio Species 0.000 description 5
FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 5
108060008682 Tumor Necrosis Factor Proteins 0.000 description 5
238000010367 cloning Methods 0.000 description 5
108020001096 dihydrofolate reductase Proteins 0.000 description 5
PRAKJMSDJKAYCZ-UHFFFAOYSA-N dodecahydrosqualene Natural products CC(C)CCCC(C)CCCC(C)CCCCC(C)CCCC(C)CCCC(C)C PRAKJMSDJKAYCZ-UHFFFAOYSA-N 0.000 description 5
239000012636 effector Substances 0.000 description 5
238000005516 engineering process Methods 0.000 description 5
230000037433 frameshift Effects 0.000 description 5
230000002519 immonomodulatory effect Effects 0.000 description 5
210000001672 ovary Anatomy 0.000 description 5
230000002829 reductive effect Effects 0.000 description 5
238000012552 review Methods 0.000 description 5
239000000243 solution Substances 0.000 description 5
230000009870 specific binding Effects 0.000 description 5
238000010561 standard procedure Methods 0.000 description 5
238000011282 treatment Methods 0.000 description 5
102000003390 tumor necrosis factor Human genes 0.000 description 5
239000003981 vehicle Substances 0.000 description 5
241000700663 Avipoxvirus Species 0.000 description 4
241001227713 Chiron Species 0.000 description 4
108091035707 Consensus sequence Proteins 0.000 description 4
DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Natural products NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 4
108010017080 Granulocyte Colony-Stimulating Factor Proteins 0.000 description 4
102000004269 Granulocyte Colony-Stimulating Factor Human genes 0.000 description 4
108700039791 Hepatitis C virus nucleocapsid Proteins 0.000 description 4
101150106931 IFNG gene Proteins 0.000 description 4
102000003815 Interleukin-11 Human genes 0.000 description 4
108090000177 Interleukin-11 Proteins 0.000 description 4
108010065805 Interleukin-12 Proteins 0.000 description 4
102000013462 Interleukin-12 Human genes 0.000 description 4
230000004988 N-glycosylation Effects 0.000 description 4
241001045988 Neogene Species 0.000 description 4
ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 4
101710192141 Protein Nef Proteins 0.000 description 4
241000714474 Rous sarcoma virus Species 0.000 description 4
241000713311 Simian immunodeficiency virus Species 0.000 description 4
108091081024 Start codon Proteins 0.000 description 4
108700019146 Transgenes Proteins 0.000 description 4
125000000539 amino acid group Chemical group 0.000 description 4
238000004458 analytical method Methods 0.000 description 4
230000000903 blocking effect Effects 0.000 description 4
201000011510 cancer Diseases 0.000 description 4
238000006243 chemical reaction Methods 0.000 description 4
239000003795 chemical substances by application Substances 0.000 description 4
230000000295 complement effect Effects 0.000 description 4
229940127089 cytotoxic agent Drugs 0.000 description 4
239000002254 cytotoxic agent Substances 0.000 description 4
231100000599 cytotoxic agent Toxicity 0.000 description 4
230000001472 cytotoxic effect Effects 0.000 description 4
238000001514 detection method Methods 0.000 description 4
239000003599 detergent Substances 0.000 description 4
238000011161 development Methods 0.000 description 4
230000004069 differentiation Effects 0.000 description 4
239000012467 final product Substances 0.000 description 4
238000001943 fluorescence-activated cell sorting Methods 0.000 description 4
BRZYSWJRSDMWLG-CAXSIQPQSA-N geneticin Chemical compound O1C[C@@](O)(C)[C@H](NC)[C@@H](O)[C@H]1O[C@@H]1[C@@H](O)[C@H](O[C@@H]2[C@@H]([C@@H](O)[C@H](O)[C@@H](C(C)O)O2)N)[C@@H](N)C[C@H]1N BRZYSWJRSDMWLG-CAXSIQPQSA-N 0.000 description 4
239000003022 immunostimulating agent Substances 0.000 description 4
230000002401 inhibitory effect Effects 0.000 description 4
229940074383 interleukin-11 Drugs 0.000 description 4
229940117681 interleukin-12 Drugs 0.000 description 4
230000003834 intracellular effect Effects 0.000 description 4
238000004255 ion exchange chromatography Methods 0.000 description 4
239000011159 matrix material Substances 0.000 description 4
239000002609 medium Substances 0.000 description 4
101150091879 neo gene Proteins 0.000 description 4
230000003287 optical effect Effects 0.000 description 4
239000013618 particulate matter Substances 0.000 description 4
229920000642 polymer Polymers 0.000 description 4
235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 description 4
229920000053 polysorbate 80 Polymers 0.000 description 4
125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 4
108020003175 receptors Proteins 0.000 description 4
238000010188 recombinant method Methods 0.000 description 4
230000009467 reduction Effects 0.000 description 4
230000001105 regulatory effect Effects 0.000 description 4
230000028327 secretion Effects 0.000 description 4
238000003786 synthesis reaction Methods 0.000 description 4
210000001519 tissue Anatomy 0.000 description 4
230000001052 transient effect Effects 0.000 description 4
238000002255 vaccination Methods 0.000 description 4
YYGNTYWPHWGJRM-UHFFFAOYSA-N (6E,10E,14E,18E)-2,6,10,15,19,23-hexamethyltetracosa-2,6,10,14,18,22-hexaene Chemical compound CC(C)=CCCC(C)=CCCC(C)=CCCC=C(C)CCC=C(C)CCC=C(C)C YYGNTYWPHWGJRM-UHFFFAOYSA-N 0.000 description 3
KSXTUUUQYQYKCR-LQDDAWAPSA-M 2,3-bis[[(z)-octadec-9-enoyl]oxy]propyl-trimethylazanium;chloride Chemical compound [Cl-].CCCCCCCC\C=C/CCCCCCCC(=O)OCC(C[N+](C)(C)C)OC(=O)CCCCCCC\C=C/CCCCCCCC KSXTUUUQYQYKCR-LQDDAWAPSA-M 0.000 description 3
UAIUNKRWKOVEES-UHFFFAOYSA-N 3,3',5,5'-tetramethylbenzidine Chemical compound CC1=C(N)C(C)=CC(C=2C=C(C)C(N)=C(C)C=2)=C1 UAIUNKRWKOVEES-UHFFFAOYSA-N 0.000 description 3
108091003079 Bovine Serum Albumin Proteins 0.000 description 3
108010041397 CD4 Antigens Proteins 0.000 description 3
108010039939 Cell Wall Skeleton Proteins 0.000 description 3
108010092408 Eosinophil Peroxidase Proteins 0.000 description 3
241000588724 Escherichia coli Species 0.000 description 3
LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 3
WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 description 3
241000233866 Fungi Species 0.000 description 3
PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 3
239000004471 Glycine Substances 0.000 description 3
208000037357 HIV infectious disease Diseases 0.000 description 3
108010002335 Interleukin-9 Proteins 0.000 description 3
102000000585 Interleukin-9 Human genes 0.000 description 3
FBOZXECLQNJBKD-ZDUSSCGKSA-N L-methotrexate Chemical compound C=1N=C2N=C(N)N=C(N)C2=NC=1CN(C)C1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 FBOZXECLQNJBKD-ZDUSSCGKSA-N 0.000 description 3
KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 3
239000004472 Lysine Substances 0.000 description 3
102100039364 Metalloproteinase inhibitor 1 Human genes 0.000 description 3
241001529936 Murinae Species 0.000 description 3
108020004711 Nucleic Acid Probes Proteins 0.000 description 3
239000012124 Opti-MEM Substances 0.000 description 3
241000288906 Primates Species 0.000 description 3
108010008281 Recombinant Fusion Proteins Proteins 0.000 description 3
102000007056 Recombinant Fusion Proteins Human genes 0.000 description 3
102000006382 Ribonucleases Human genes 0.000 description 3
108010083644 Ribonucleases Proteins 0.000 description 3
241000710961 Semliki Forest virus Species 0.000 description 3
241000700584 Simplexvirus Species 0.000 description 3
241000710960 Sindbis virus Species 0.000 description 3
BHEOSNUKNHRBNM-UHFFFAOYSA-N Tetramethylsqualene Natural products CC(=C)C(C)CCC(=C)C(C)CCC(C)=CCCC=C(C)CCC(C)C(=C)CCC(C)C(C)=C BHEOSNUKNHRBNM-UHFFFAOYSA-N 0.000 description 3
102000006601 Thymidine Kinase Human genes 0.000 description 3
108020004440 Thymidine kinase Proteins 0.000 description 3
238000001042 affinity chromatography Methods 0.000 description 3
230000003321 amplification Effects 0.000 description 3
125000000129 anionic group Chemical group 0.000 description 3
210000000612 antigen-presenting cell Anatomy 0.000 description 3
210000003651 basophil Anatomy 0.000 description 3
230000004071 biological effect Effects 0.000 description 3
230000034303 cell budding Effects 0.000 description 3
239000013592 cell lysate Substances 0.000 description 3
210000004520 cell wall skeleton Anatomy 0.000 description 3
238000012761 co-transfection Methods 0.000 description 3
239000000356 contaminant Substances 0.000 description 3
230000001086 cytosolic effect Effects 0.000 description 3
231100000433 cytotoxic Toxicity 0.000 description 3
230000002939 deleterious effect Effects 0.000 description 3
201000010099 disease Diseases 0.000 description 3
208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 3
230000003828 downregulation Effects 0.000 description 3
239000003814 drug Substances 0.000 description 3
230000008030 elimination Effects 0.000 description 3
238000003379 elimination reaction Methods 0.000 description 3
239000000839 emulsion Substances 0.000 description 3
210000003979 eosinophil Anatomy 0.000 description 3
238000011156 evaluation Methods 0.000 description 3
239000012894 fetal calf serum Substances 0.000 description 3
238000005194 fractionation Methods 0.000 description 3
108020001507 fusion proteins Proteins 0.000 description 3
102000037865 fusion proteins Human genes 0.000 description 3
238000012239 gene modification Methods 0.000 description 3
230000005017 genetic modification Effects 0.000 description 3
235000013617 genetically modified food Nutrition 0.000 description 3
ZDXPYRJPNDTMRX-UHFFFAOYSA-N glutamine Natural products OC(=O)C(N)CCC(N)=O ZDXPYRJPNDTMRX-UHFFFAOYSA-N 0.000 description 3
230000012010 growth Effects 0.000 description 3
208000033519 human immunodeficiency virus infectious disease Diseases 0.000 description 3
230000010354 integration Effects 0.000 description 3
238000007918 intramuscular administration Methods 0.000 description 3
238000002955 isolation Methods 0.000 description 3
238000001823 molecular biology technique Methods 0.000 description 3
210000001616 monocyte Anatomy 0.000 description 3
238000003199 nucleic acid amplification method Methods 0.000 description 3
239000002853 nucleic acid probe Substances 0.000 description 3
244000045947 parasite Species 0.000 description 3
230000036961 partial effect Effects 0.000 description 3
210000005105 peripheral blood lymphocyte Anatomy 0.000 description 3
230000002516 postimmunization Effects 0.000 description 3
230000001124 posttranscriptional effect Effects 0.000 description 3
238000001556 precipitation Methods 0.000 description 3
238000011809 primate model Methods 0.000 description 3
230000008569 process Effects 0.000 description 3
102000005962 receptors Human genes 0.000 description 3
108091008146 restriction endonucleases Proteins 0.000 description 3
150000003839 salts Chemical class 0.000 description 3
238000000926 separation method Methods 0.000 description 3
239000013605 shuttle vector Substances 0.000 description 3
239000011780 sodium chloride Substances 0.000 description 3
229940031439 squalene Drugs 0.000 description 3
TUHBEKDERLKLEC-UHFFFAOYSA-N squalene Natural products CC(=CCCC(=CCCC(=CCCC=C(/C)CCC=C(/C)CC=C(C)C)C)C)C TUHBEKDERLKLEC-UHFFFAOYSA-N 0.000 description 3
229940031626 subunit vaccine Drugs 0.000 description 3
239000003053 toxin Substances 0.000 description 3
231100000765 toxin Toxicity 0.000 description 3
108700012359 toxins Proteins 0.000 description 3
230000014621 translational initiation Effects 0.000 description 3
YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 2
SNKAWJBJQDLSFF-NVKMUCNASA-N 1,2-dioleoyl-sn-glycero-3-phosphocholine Chemical compound CCCCCCCC\C=C/CCCCCCCC(=O)OC[C@H](COP([O-])(=O)OCC[N+](C)(C)C)OC(=O)CCCCCCC\C=C/CCCCCCCC SNKAWJBJQDLSFF-NVKMUCNASA-N 0.000 description 2
LDGWQMRUWMSZIU-LQDDAWAPSA-M 2,3-bis[(z)-octadec-9-enoxy]propyl-trimethylazanium;chloride Chemical compound [Cl-].CCCCCCCC\C=C/CCCCCCCCOCC(C[N+](C)(C)C)OCCCCCCCC\C=C/CCCCCCCC LDGWQMRUWMSZIU-LQDDAWAPSA-M 0.000 description 2
HZAXFHJVJLSVMW-UHFFFAOYSA-N 2-Aminoethan-1-ol Chemical compound NCCO HZAXFHJVJLSVMW-UHFFFAOYSA-N 0.000 description 2
241000272517 Anseriformes Species 0.000 description 2
239000004475 Arginine Substances 0.000 description 2
IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
102000017420 CD3 protein, epsilon/gamma/delta subunit Human genes 0.000 description 2
108050005493 CD3 protein, epsilon/gamma/delta subunit Proteins 0.000 description 2
241000283707 Capra Species 0.000 description 2
101710132601 Capsid protein Proteins 0.000 description 2
241000282693 Cercopithecidae Species 0.000 description 2
LZZYPRNAOMGNLH-UHFFFAOYSA-M Cetrimonium bromide Chemical compound [Br-].CCCCCCCCCCCCCCCC[N+](C)(C)C LZZYPRNAOMGNLH-UHFFFAOYSA-M 0.000 description 2
108010049048 Cholera Toxin Proteins 0.000 description 2
102000009016 Cholera Toxin Human genes 0.000 description 2
102000011022 Chorionic Gonadotropin Human genes 0.000 description 2
108010062540 Chorionic Gonadotropin Proteins 0.000 description 2
102000000311 Cytosine Deaminase Human genes 0.000 description 2
108010080611 Cytosine Deaminase Proteins 0.000 description 2
101150074155 DHFR gene Proteins 0.000 description 2
229940021995 DNA vaccine Drugs 0.000 description 2
229920002307 Dextran Polymers 0.000 description 2
206010014611 Encephalitis venezuelan equine Diseases 0.000 description 2
101710091045 Envelope protein Proteins 0.000 description 2
101000686777 Escherichia phage T7 T7 RNA polymerase Proteins 0.000 description 2
108091029865 Exogenous DNA Proteins 0.000 description 2
108700024394 Exon Proteins 0.000 description 2
241000713800 Feline immunodeficiency virus Species 0.000 description 2
ZHNUHDYFZUAESO-UHFFFAOYSA-N Formamide Chemical compound NC=O ZHNUHDYFZUAESO-UHFFFAOYSA-N 0.000 description 2
108700007698 Genetic Terminator Regions Proteins 0.000 description 2
108010010369 HIV Protease Proteins 0.000 description 2
229940033330 HIV vaccine Drugs 0.000 description 2
229940033332 HIV-1 vaccine Drugs 0.000 description 2
101000917383 Homo sapiens Deoxycytidine kinase Proteins 0.000 description 2
102100034349 Integrase Human genes 0.000 description 2
101710203526 Integrase Proteins 0.000 description 2
102000006992 Interferon-alpha Human genes 0.000 description 2
108010047761 Interferon-alpha Proteins 0.000 description 2
102000003996 Interferon-beta Human genes 0.000 description 2
108090000467 Interferon-beta Proteins 0.000 description 2
102000008070 Interferon-gamma Human genes 0.000 description 2
108010074328 Interferon-gamma Proteins 0.000 description 2
102000003814 Interleukin-10 Human genes 0.000 description 2
108090000174 Interleukin-10 Proteins 0.000 description 2
102000003812 Interleukin-15 Human genes 0.000 description 2
102000004125 Interleukin-1alpha Human genes 0.000 description 2
108010082786 Interleukin-1alpha Proteins 0.000 description 2
108010002586 Interleukin-7 Proteins 0.000 description 2
108090001007 Interleukin-8 Proteins 0.000 description 2
102000004890 Interleukin-8 Human genes 0.000 description 2
WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 description 2
AGPKZVBTJJNPAG-WHFBIAKZSA-N L-isoleucine Chemical compound CC[C@H](C)[C@H](N)C(O)=O AGPKZVBTJJNPAG-WHFBIAKZSA-N 0.000 description 2
ROHFNLRQFUQHCH-YFKPBYRVSA-N L-leucine Chemical compound CC(C)C[C@H](N)C(O)=O ROHFNLRQFUQHCH-YFKPBYRVSA-N 0.000 description 2
COLNVLDHVKWLRT-QMMMGPOBSA-N L-phenylalanine Chemical compound OC(=O)[C@@H](N)CC1=CC=CC=C1 COLNVLDHVKWLRT-QMMMGPOBSA-N 0.000 description 2
QIVBCDIJIAJPQS-VIFPVBQESA-N L-tryptophane Chemical compound C1=CC=C2C(C[C@H](N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-VIFPVBQESA-N 0.000 description 2
OUYCCCASQSFEME-QMMMGPOBSA-N L-tyrosine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-QMMMGPOBSA-N 0.000 description 2
241000124008 Mammalia Species 0.000 description 2
241000713333 Mouse mammary tumor virus Species 0.000 description 2
241000714177 Murine leukemia virus Species 0.000 description 2
108700020354 N-acetylmuramyl-threonyl-isoglutamine Proteins 0.000 description 2
229930193140 Neomycin Natural products 0.000 description 2
108020003217 Nuclear RNA Proteins 0.000 description 2
102000043141 Nuclear RNA Human genes 0.000 description 2
108700026244 Open Reading Frames Proteins 0.000 description 2
108010081690 Pertussis Toxin Proteins 0.000 description 2
241000235648 Pichia Species 0.000 description 2
239000002202 Polyethylene glycol Substances 0.000 description 2
ATUOYWHBWRKTHZ-UHFFFAOYSA-N Propane Chemical compound CCC ATUOYWHBWRKTHZ-UHFFFAOYSA-N 0.000 description 2
101710150344 Protein Rev Proteins 0.000 description 2
108010076504 Protein Sorting Signals Proteins 0.000 description 2
101710188315 Protein X Proteins 0.000 description 2
108700005075 Regulator Genes Proteins 0.000 description 2
241001068263 Replication competent viruses Species 0.000 description 2
241000712907 Retroviridae Species 0.000 description 2
206010039897 Sedation Diseases 0.000 description 2
MTCFGRXMJLQNBG-UHFFFAOYSA-N Serine Natural products OCC(N)C(O)=O MTCFGRXMJLQNBG-UHFFFAOYSA-N 0.000 description 2
PRXRUNOAOLTIEF-ADSICKODSA-N Sorbitan trioleate Chemical compound CCCCCCCC\C=C/CCCCCCCC(=O)OC[C@@H](OC(=O)CCCCCCC\C=C/CCCCCCCC)[C@H]1OC[C@H](O)[C@H]1OC(=O)CCCCCCC\C=C/CCCCCCCC PRXRUNOAOLTIEF-ADSICKODSA-N 0.000 description 2
229930006000 Sucrose Natural products 0.000 description 2
CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 2
102000036693 Thrombopoietin Human genes 0.000 description 2
108010041111 Thrombopoietin Proteins 0.000 description 2
241000255993 Trichoplusia ni Species 0.000 description 2
QIVBCDIJIAJPQS-UHFFFAOYSA-N Tryptophan Natural products C1=CC=C2C(CC(N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-UHFFFAOYSA-N 0.000 description 2
208000002687 Venezuelan Equine Encephalomyelitis Diseases 0.000 description 2
201000009145 Venezuelan equine encephalitis Diseases 0.000 description 2
DSNRWDQKZIEDDB-GCMPNPAFSA-N [(2r)-3-[2,3-dihydroxypropoxy(hydroxy)phosphoryl]oxy-2-[(z)-octadec-9-enoyl]oxypropyl] (z)-octadec-9-enoate Chemical compound CCCCCCCC\C=C/CCCCCCCC(=O)OC[C@H](COP(O)(=O)OCC(O)CO)OC(=O)CCCCCCC\C=C/CCCCCCCC DSNRWDQKZIEDDB-GCMPNPAFSA-N 0.000 description 2
230000002411 adverse Effects 0.000 description 2
229940037003 alum Drugs 0.000 description 2
239000012491 analyte Substances 0.000 description 2
238000010171 animal model Methods 0.000 description 2
239000005557 antagonist Substances 0.000 description 2
230000036436 anti-hiv Effects 0.000 description 2
230000010056 antibody-dependent cellular cytotoxicity Effects 0.000 description 2
ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 description 2
210000004899 c-terminal region Anatomy 0.000 description 2
AIYUHDOJVYHVIT-UHFFFAOYSA-M caesium chloride Chemical compound [Cl-].[Cs+] AIYUHDOJVYHVIT-UHFFFAOYSA-M 0.000 description 2
239000001506 calcium phosphate Substances 0.000 description 2
229910000389 calcium phosphate Inorganic materials 0.000 description 2
235000011010 calcium phosphates Nutrition 0.000 description 2
125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 2
238000004113 cell culture Methods 0.000 description 2
210000000170 cell membrane Anatomy 0.000 description 2
210000002421 cell wall Anatomy 0.000 description 2
230000007969 cellular immunity Effects 0.000 description 2
HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 2
239000007979 citrate buffer Substances 0.000 description 2
150000001875 compounds Chemical class 0.000 description 2
238000007796 conventional method Methods 0.000 description 2
230000002596 correlated effect Effects 0.000 description 2
230000009089 cytolysis Effects 0.000 description 2
210000000805 cytoplasm Anatomy 0.000 description 2
230000003247 decreasing effect Effects 0.000 description 2
230000002950 deficient Effects 0.000 description 2
210000004443 dendritic cell Anatomy 0.000 description 2
230000001419 dependent effect Effects 0.000 description 2
229960002086 dextran Drugs 0.000 description 2
229940079593 drug Drugs 0.000 description 2
238000005538 encapsulation Methods 0.000 description 2
108700004025 env Genes Proteins 0.000 description 2
239000013613 expression plasmid Substances 0.000 description 2
238000000605 extraction Methods 0.000 description 2
238000010230 functional analysis Methods 0.000 description 2
230000004927 fusion Effects 0.000 description 2
229940044627 gamma-interferon Drugs 0.000 description 2
108091006104 gene-regulatory proteins Proteins 0.000 description 2
102000034356 gene-regulatory proteins Human genes 0.000 description 2
230000036541 health Effects 0.000 description 2
238000002744 homologous recombination Methods 0.000 description 2
230000006801 homologous recombination Effects 0.000 description 2
229940084986 human chorionic gonadotropin Drugs 0.000 description 2
238000003018 immunoassay Methods 0.000 description 2
229940127121 immunoconjugate Drugs 0.000 description 2
230000003308 immunostimulating effect Effects 0.000 description 2
230000001771 impaired effect Effects 0.000 description 2
230000006872 improvement Effects 0.000 description 2
230000002458 infectious effect Effects 0.000 description 2
238000001802 infusion Methods 0.000 description 2
238000010255 intramuscular injection Methods 0.000 description 2
239000007927 intramuscular injection Substances 0.000 description 2
229930027917 kanamycin Natural products 0.000 description 2
229960000318 kanamycin Drugs 0.000 description 2
SBUJHOSQTJFQJX-NOAMYHISSA-N kanamycin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CN)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](N)[C@H](O)[C@@H](CO)O2)O)[C@H](N)C[C@@H]1N SBUJHOSQTJFQJX-NOAMYHISSA-N 0.000 description 2
229930182823 kanamycin A Natural products 0.000 description 2
210000000265 leukocyte Anatomy 0.000 description 2
230000021633 leukocyte mediated immunity Effects 0.000 description 2
239000006166 lysate Substances 0.000 description 2
229920002521 macromolecule Polymers 0.000 description 2
230000035800 maturation Effects 0.000 description 2
238000005259 measurement Methods 0.000 description 2
239000012528 membrane Substances 0.000 description 2
229960000485 methotrexate Drugs 0.000 description 2
JMUHBNWAORSSBD-WKYWBUFDSA-N mifamurtide Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@@H](OC(=O)CCCCCCCCCCCCCCC)COP(O)(=O)OCCNC(=O)[C@H](C)NC(=O)CC[C@H](C(N)=O)NC(=O)[C@H](C)NC(=O)[C@@H](C)O[C@H]1[C@H](O)[C@@H](CO)OC(O)[C@@H]1NC(C)=O JMUHBNWAORSSBD-WKYWBUFDSA-N 0.000 description 2
229960005225 mifamurtide Drugs 0.000 description 2
239000003226 mitogen Substances 0.000 description 2
238000010369 molecular cloning Methods 0.000 description 2
125000001446 muramyl group Chemical group N[C@@H](C=O)[C@@H](O[C@@H](C(=O)*)C)[C@H](O)[C@H](O)CO 0.000 description 2
UPBAOYRENQEPJO-UHFFFAOYSA-N n-[5-[[5-[(3-amino-3-iminopropyl)carbamoyl]-1-methylpyrrol-3-yl]carbamoyl]-1-methylpyrrol-3-yl]-4-formamido-1-methylpyrrole-2-carboxamide Chemical compound CN1C=C(NC=O)C=C1C(=O)NC1=CN(C)C(C(=O)NC2=CN(C)C(C(=O)NCCC(N)=N)=C2)=C1 UPBAOYRENQEPJO-UHFFFAOYSA-N 0.000 description 2
229960004927 neomycin Drugs 0.000 description 2
IDBIFFKSXLYUOT-UHFFFAOYSA-N netropsin Chemical compound C1=C(C(=O)NCCC(N)=N)N(C)C=C1NC(=O)C1=CC(NC(=O)CN=C(N)N)=CN1C IDBIFFKSXLYUOT-UHFFFAOYSA-N 0.000 description 2
230000007935 neutral effect Effects 0.000 description 2
229910052757 nitrogen Inorganic materials 0.000 description 2
230000009871 nonspecific binding Effects 0.000 description 2
231100000252 nontoxic Toxicity 0.000 description 2
230000003000 nontoxic effect Effects 0.000 description 2
230000008520 organization Effects 0.000 description 2
239000000546 pharmaceutical excipient Substances 0.000 description 2
COLNVLDHVKWLRT-UHFFFAOYSA-N phenylalanine Natural products OC(=O)C(N)CC1=CC=CC=C1 COLNVLDHVKWLRT-UHFFFAOYSA-N 0.000 description 2
239000002953 phosphate buffered saline Substances 0.000 description 2
239000013600 plasmid vector Substances 0.000 description 2
210000001778 pluripotent stem cell Anatomy 0.000 description 2
229920003229 poly(methyl methacrylate) Polymers 0.000 description 2
229920001223 polyethylene glycol Polymers 0.000 description 2
229920000136 polysorbate Polymers 0.000 description 2
230000037452 priming Effects 0.000 description 2
230000001681 protective effect Effects 0.000 description 2
229940023143 protein vaccine Drugs 0.000 description 2
238000011160 research Methods 0.000 description 2
238000010839 reverse transcription Methods 0.000 description 2
230000002441 reversible effect Effects 0.000 description 2
238000013341 scale-up Methods 0.000 description 2
238000012216 screening Methods 0.000 description 2
230000036280 sedation Effects 0.000 description 2
238000004062 sedimentation Methods 0.000 description 2
238000002741 site-directed mutagenesis Methods 0.000 description 2
239000007787 solid Substances 0.000 description 2
241000894007 species Species 0.000 description 2
ATHGHQPFGPMSJY-UHFFFAOYSA-N spermidine Chemical compound NCCCCNCCCN ATHGHQPFGPMSJY-UHFFFAOYSA-N 0.000 description 2
PFNFFQXMRSDOHW-UHFFFAOYSA-N spermine Chemical compound NCCCNCCCCNCCCN PFNFFQXMRSDOHW-UHFFFAOYSA-N 0.000 description 2
239000007858 starting material Substances 0.000 description 2
239000005720 sucrose Substances 0.000 description 2
208000024891 symptom Diseases 0.000 description 2
208000011580 syndromic disease Diseases 0.000 description 2
238000012360 testing method Methods 0.000 description 2
230000001225 therapeutic effect Effects 0.000 description 2
239000012096 transfection reagent Substances 0.000 description 2
XETCRXVKJHBPMK-MJSODCSWSA-N trehalose 6,6'-dimycolate Chemical compound C([C@@H]1[C@H]([C@H](O)[C@@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](O)[C@H](O)[C@@H](COC(=O)C(CCCCCCCCCCC3C(C3)CCCCCCCCCCCCCCCCCC)C(O)CCCCCCCCCCCCCCCCCCCCCCCCC)O2)O)O1)O)OC(=O)C(C(O)CCCCCCCCCCCCCCCCCCCCCCCCC)CCCCCCCCCCC1CC1CCCCCCCCCCCCCCCCCC XETCRXVKJHBPMK-MJSODCSWSA-N 0.000 description 2
QORWJWZARLRLPR-UHFFFAOYSA-H tricalcium bis(phosphate) Chemical compound [Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O QORWJWZARLRLPR-UHFFFAOYSA-H 0.000 description 2
OUYCCCASQSFEME-UHFFFAOYSA-N tyrosine Natural products OC(=O)C(N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-UHFFFAOYSA-N 0.000 description 2
239000002691 unilamellar liposome Substances 0.000 description 2
238000011144 upstream manufacturing Methods 0.000 description 2
230000029812 viral genome replication Effects 0.000 description 2
210000002845 virion Anatomy 0.000 description 2
229960001600 xylazine Drugs 0.000 description 2
KIUKXJAPPMFGSW-DNGZLQJQSA-N (2S,3S,4S,5R,6R)-6-[(2S,3R,4R,5S,6R)-3-Acetamido-2-[(2S,3S,4R,5R,6R)-6-[(2R,3R,4R,5S,6R)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2-carboxylic acid Chemical compound CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 KIUKXJAPPMFGSW-DNGZLQJQSA-N 0.000 description 1
YUXKOWPNKJSTPQ-AXWWPMSFSA-N (2s,3r)-2-amino-3-hydroxybutanoic acid;(2s)-2-amino-3-hydroxypropanoic acid Chemical compound OC[C@H](N)C(O)=O.C[C@@H](O)[C@H](N)C(O)=O YUXKOWPNKJSTPQ-AXWWPMSFSA-N 0.000 description 1
YHQZWWDVLJPRIF-JLHRHDQISA-N (4R)-4-[[(2S,3R)-2-[acetyl-[(3R,4R,5S,6R)-3-amino-4-[(1R)-1-carboxyethoxy]-5-hydroxy-6-(hydroxymethyl)oxan-2-yl]amino]-3-hydroxybutanoyl]amino]-5-amino-5-oxopentanoic acid Chemical compound C(C)(=O)N([C@@H]([C@H](O)C)C(=O)N[C@H](CCC(=O)O)C(N)=O)C1[C@H](N)[C@@H](O[C@@H](C(=O)O)C)[C@H](O)[C@H](O1)CO YHQZWWDVLJPRIF-JLHRHDQISA-N 0.000 description 1
LEBVLXFERQHONN-UHFFFAOYSA-N 1-butyl-N-(2,6-dimethylphenyl)piperidine-2-carboxamide Chemical compound CCCCN1CCCCC1C(=O)NC1=C(C)C=CC=C1C LEBVLXFERQHONN-UHFFFAOYSA-N 0.000 description 1
QRBLKGHRWFGINE-UGWAGOLRSA-N 2-[2-[2-[[2-[[4-[[2-[[6-amino-2-[3-amino-1-[(2,3-diamino-3-oxopropyl)amino]-3-oxopropyl]-5-methylpyrimidine-4-carbonyl]amino]-3-[(2r,3s,4s,5s,6s)-3-[(2s,3r,4r,5s)-4-carbamoyl-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxy-4,5-dihydroxy-6-(hydroxymethyl)- Chemical compound N=1C(C=2SC=C(N=2)C(N)=O)CSC=1CCNC(=O)C(C(C)=O)NC(=O)C(C)C(O)C(C)NC(=O)C(C(O[C@H]1[C@@]([C@@H](O)[C@H](O)[C@H](CO)O1)(C)O[C@H]1[C@@H]([C@](O)([C@@H](O)C(CO)O1)C(N)=O)O)C=1NC=NC=1)NC(=O)C1=NC(C(CC(N)=O)NCC(N)C(N)=O)=NC(N)=C1C QRBLKGHRWFGINE-UGWAGOLRSA-N 0.000 description 1
BFSVOASYOCHEOV-UHFFFAOYSA-N 2-diethylaminoethanol Chemical compound CCN(CC)CCO BFSVOASYOCHEOV-UHFFFAOYSA-N 0.000 description 1
101150084399 37 gene Proteins 0.000 description 1
UZOVYGYOLBIAJR-UHFFFAOYSA-N 4-isocyanato-4'-methyldiphenylmethane Chemical compound C1=CC(C)=CC=C1CC1=CC=C(N=C=O)C=C1 UZOVYGYOLBIAJR-UHFFFAOYSA-N 0.000 description 1
WOVKYSAHUYNSMH-RRKCRQDMSA-N 5-bromodeoxyuridine Chemical compound C1[C@H](O)[C@@H](CO)O[C@H]1N1C(=O)NC(=O)C(Br)=C1 WOVKYSAHUYNSMH-RRKCRQDMSA-N 0.000 description 1
101150088993 62 gene Proteins 0.000 description 1
KDCGOANMDULRCW-UHFFFAOYSA-N 7H-purine Chemical compound N1=CNC2=NC=NC2=C1 KDCGOANMDULRCW-UHFFFAOYSA-N 0.000 description 1
239000013607 AAV vector Substances 0.000 description 1
208000024893 Acute lymphoblastic leukemia Diseases 0.000 description 1
208000014697 Acute lymphocytic leukaemia Diseases 0.000 description 1
241000256118 Aedes aegypti Species 0.000 description 1
241000714195 Aids-associated retrovirus Species 0.000 description 1
239000005995 Aluminium silicate Substances 0.000 description 1
DCXYFEDJOCDNAF-UHFFFAOYSA-N Asparagine Natural products OC(=O)C(N)CC(N)=O DCXYFEDJOCDNAF-UHFFFAOYSA-N 0.000 description 1
241001203868 Autographa californica Species 0.000 description 1
101150076489 B gene Proteins 0.000 description 1
238000011725 BALB/c mouse Methods 0.000 description 1
WOVKYSAHUYNSMH-UHFFFAOYSA-N BROMODEOXYURIDINE Natural products C1C(O)C(CO)OC1N1C(=O)NC(=O)C(Br)=C1 WOVKYSAHUYNSMH-UHFFFAOYSA-N 0.000 description 1
244000063299 Bacillus subtilis Species 0.000 description 1
235000014469 Bacillus subtilis Nutrition 0.000 description 1
241000283690 Bos taurus Species 0.000 description 1
102220604177 C-X-C chemokine receptor type 6_D25A_mutation Human genes 0.000 description 1
108010017088 CCR5 Receptors Proteins 0.000 description 1
102000004274 CCR5 Receptors Human genes 0.000 description 1
101150017501 CCR5 gene Proteins 0.000 description 1
210000004366 CD4-positive T-lymphocyte Anatomy 0.000 description 1
210000001266 CD8-positive T-lymphocyte Anatomy 0.000 description 1
QCMYYKRYFNMIEC-UHFFFAOYSA-N COP(O)=O Chemical class COP(O)=O QCMYYKRYFNMIEC-UHFFFAOYSA-N 0.000 description 1
108010061299 CXCR4 Receptors Proteins 0.000 description 1
102000012000 CXCR4 Receptors Human genes 0.000 description 1
108010061304 CXCR6 Receptors Proteins 0.000 description 1
102000011968 CXCR6 Receptors Human genes 0.000 description 1
101100152433 Caenorhabditis elegans tat-1 gene Proteins 0.000 description 1
241000178270 Canarypox virus Species 0.000 description 1
241000222122 Candida albicans Species 0.000 description 1
241000222128 Candida maltosa Species 0.000 description 1
241000282472 Canis lupus familiaris Species 0.000 description 1
108010012236 Chemokines Proteins 0.000 description 1
102000019034 Chemokines Human genes 0.000 description 1
206010008631 Cholera Diseases 0.000 description 1
241000251556 Chordata Species 0.000 description 1
VYZAMTAEIAYCRO-UHFFFAOYSA-N Chromium Chemical compound [Cr] VYZAMTAEIAYCRO-UHFFFAOYSA-N 0.000 description 1
KRKNYBCHXYNGOX-UHFFFAOYSA-K Citrate Chemical compound [O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O KRKNYBCHXYNGOX-UHFFFAOYSA-K 0.000 description 1
101710094648 Coat protein Proteins 0.000 description 1
241000557626 Corvus corax Species 0.000 description 1
241000699800 Cricetinae Species 0.000 description 1
108010072220 Cyclophilin A Proteins 0.000 description 1
CMSMOCZEIVJLDB-UHFFFAOYSA-N Cyclophosphamide Chemical compound ClCCN(CCCl)P1(=O)NCCCO1 CMSMOCZEIVJLDB-UHFFFAOYSA-N 0.000 description 1
UHDGCWIWMRVCDJ-CCXZUQQUSA-N Cytarabine Chemical compound O=C1N=C(N)C=CN1[C@H]1[C@@H](O)[C@H](O)[C@@H](CO)O1 UHDGCWIWMRVCDJ-CCXZUQQUSA-N 0.000 description 1
108010020076 Cytochrome P-450 CYP2B1 Proteins 0.000 description 1
108010015742 Cytochrome P-450 Enzyme System Proteins 0.000 description 1
102000003849 Cytochrome P450 Human genes 0.000 description 1
102000053602 DNA Human genes 0.000 description 1
108010041986 DNA Vaccines Proteins 0.000 description 1
230000004544 DNA amplification Effects 0.000 description 1
239000003155 DNA primer Substances 0.000 description 1
238000001712 DNA sequencing Methods 0.000 description 1
230000004568 DNA-binding Effects 0.000 description 1
108090000626 DNA-directed RNA polymerases Proteins 0.000 description 1
102000004163 DNA-directed RNA polymerases Human genes 0.000 description 1
108010092160 Dactinomycin Proteins 0.000 description 1
241000702421 Dependoparvovirus Species 0.000 description 1
241000255581 Drosophila <fruit fly, genus> Species 0.000 description 1
101100136092 Drosophila melanogaster peng gene Proteins 0.000 description 1
101100117236 Drosophila melanogaster speck gene Proteins 0.000 description 1
101710117538 Endogenous retrovirus group FC1 Env polyprotein Proteins 0.000 description 1
101710167714 Endogenous retrovirus group K member 18 Env polyprotein Proteins 0.000 description 1
101710152279 Endogenous retrovirus group K member 21 Env polyprotein Proteins 0.000 description 1
101710197529 Endogenous retrovirus group K member 25 Env polyprotein Proteins 0.000 description 1
101710141424 Endogenous retrovirus group K member 6 Env polyprotein Proteins 0.000 description 1
101710159911 Endogenous retrovirus group K member 8 Env polyprotein Proteins 0.000 description 1
101710205628 Endogenous retrovirus group K member 9 Env polyprotein Proteins 0.000 description 1
YQYJSBFKSSDGFO-UHFFFAOYSA-N Epihygromycin Natural products OC1C(O)C(C(=O)C)OC1OC(C(=C1)O)=CC=C1C=C(C)C(=O)NC1C(O)C(O)C2OCOC2C1O YQYJSBFKSSDGFO-UHFFFAOYSA-N 0.000 description 1
241000283086 Equidae Species 0.000 description 1
241000282326 Felis catus Species 0.000 description 1
238000012413 Fluorescence activated cell sorting analysis Methods 0.000 description 1
208000000666 Fowlpox Diseases 0.000 description 1
241000700662 Fowlpox virus Species 0.000 description 1
241000272496 Galliformes Species 0.000 description 1
241000287828 Gallus gallus Species 0.000 description 1
108700023863 Gene Components Proteins 0.000 description 1
JZNWSCPGTDBMEW-UHFFFAOYSA-N Glycerophosphorylethanolamin Natural products NCCOP(O)(=O)OCC(O)CO JZNWSCPGTDBMEW-UHFFFAOYSA-N 0.000 description 1
102100021181 Golgi phosphoprotein 3 Human genes 0.000 description 1
208000032843 Hemorrhage Diseases 0.000 description 1
208000005176 Hepatitis C Diseases 0.000 description 1
229920000209 Hexadimethrine bromide Polymers 0.000 description 1
108010033040 Histones Proteins 0.000 description 1
241001272567 Hominoidea Species 0.000 description 1
241000282412 Homo Species 0.000 description 1
101001050288 Homo sapiens Transcription factor Jun Proteins 0.000 description 1
108010000521 Human Growth Hormone Proteins 0.000 description 1
102000002265 Human Growth Hormone Human genes 0.000 description 1
239000000854 Human Growth Hormone Substances 0.000 description 1
241000598436 Human T-cell lymphotropic virus Species 0.000 description 1
108700020135 Human immunodeficiency virus 1 vpu Proteins 0.000 description 1
108010060231 Insect Proteins Proteins 0.000 description 1
102000000589 Interleukin-1 Human genes 0.000 description 1
108010002352 Interleukin-1 Proteins 0.000 description 1
102000003816 Interleukin-13 Human genes 0.000 description 1
108090000176 Interleukin-13 Proteins 0.000 description 1
102000000704 Interleukin-7 Human genes 0.000 description 1
102000015696 Interleukins Human genes 0.000 description 1
108010063738 Interleukins Proteins 0.000 description 1
108091092195 Intron Proteins 0.000 description 1
108010025815 Kanamycin Kinase Proteins 0.000 description 1
102100020880 Kit ligand Human genes 0.000 description 1
241001138401 Kluyveromyces lactis Species 0.000 description 1
ZQISRDCJNBUVMM-UHFFFAOYSA-N L-Histidinol Natural products OCC(N)CC1=CN=CN1 ZQISRDCJNBUVMM-UHFFFAOYSA-N 0.000 description 1
ONIBWKKTOPOVIA-BYPYZUCNSA-N L-Proline Chemical compound OC(=O)[C@@H]1CCCN1 ONIBWKKTOPOVIA-BYPYZUCNSA-N 0.000 description 1
ODKSFYDXXFIFQN-BYPYZUCNSA-P L-argininium(2+) Chemical compound NC(=[NH2+])NCCC[C@H]([NH3+])C(O)=O ODKSFYDXXFIFQN-BYPYZUCNSA-P 0.000 description 1
DCXYFEDJOCDNAF-REOHCLBHSA-N L-asparagine Chemical compound OC(=O)[C@@H](N)CC(N)=O DCXYFEDJOCDNAF-REOHCLBHSA-N 0.000 description 1
CKLJMWTZIZZHCS-REOHCLBHSA-N L-aspartic acid Chemical compound OC(=O)[C@@H](N)CC(O)=O CKLJMWTZIZZHCS-REOHCLBHSA-N 0.000 description 1
LEVWYRKDKASIDU-IMJSIDKUSA-N L-cystine Chemical compound [O-]C(=O)[C@@H]([NH3+])CSSC[C@H]([NH3+])C([O-])=O LEVWYRKDKASIDU-IMJSIDKUSA-N 0.000 description 1
ZDXPYRJPNDTMRX-VKHMYHEASA-N L-glutamine Chemical compound OC(=O)[C@@H](N)CCC(N)=O ZDXPYRJPNDTMRX-VKHMYHEASA-N 0.000 description 1
HNDVDQJCIGZPNO-YFKPBYRVSA-N L-histidine Chemical compound OC(=O)[C@@H](N)CC1=CN=CN1 HNDVDQJCIGZPNO-YFKPBYRVSA-N 0.000 description 1
ZQISRDCJNBUVMM-YFKPBYRVSA-N L-histidinol Chemical compound OC[C@@H](N)CC1=CNC=N1 ZQISRDCJNBUVMM-YFKPBYRVSA-N 0.000 description 1
FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 description 1
KZSNJWFQEVHDMF-BYPYZUCNSA-N L-valine Chemical compound CC(C)[C@H](N)C(O)=O KZSNJWFQEVHDMF-BYPYZUCNSA-N 0.000 description 1
ROHFNLRQFUQHCH-UHFFFAOYSA-N Leucine Natural products CC(C)CC(N)C(O)=O ROHFNLRQFUQHCH-UHFFFAOYSA-N 0.000 description 1
102000004058 Leukemia inhibitory factor Human genes 0.000 description 1
108090000581 Leukemia inhibitory factor Proteins 0.000 description 1
208000008771 Lymphadenopathy Diseases 0.000 description 1
241000282553 Macaca Species 0.000 description 1
210000002361 Megakaryocyte Progenitor Cell Anatomy 0.000 description 1
102000018697 Membrane Proteins Human genes 0.000 description 1
108010052285 Membrane Proteins Proteins 0.000 description 1
QXOHLNCNYLGICT-YFKPBYRVSA-N Met-Gly Chemical compound CSCC[C@H](N)C(=O)NCC(O)=O QXOHLNCNYLGICT-YFKPBYRVSA-N 0.000 description 1
108091007491 NSP3 Papain-like protease domains Proteins 0.000 description 1
101000783356 Naja sputatrix Cytotoxin Proteins 0.000 description 1
108700019961 Neoplasm Genes Proteins 0.000 description 1
102000048850 Neoplasm Genes Human genes 0.000 description 1
108010042309 Netropsin Proteins 0.000 description 1
238000000636 Northern blotting Methods 0.000 description 1
101710141454 Nucleoprotein Proteins 0.000 description 1
241000320412 Ogataea angusta Species 0.000 description 1
108700020796 Oncogene Proteins 0.000 description 1
238000012408 PCR amplification Methods 0.000 description 1
241000282579 Pan Species 0.000 description 1
241000282516 Papio anubis Species 0.000 description 1
241001494479 Pecora Species 0.000 description 1
102000035195 Peptidases Human genes 0.000 description 1
102100034539 Peptidyl-prolyl cis-trans isomerase A Human genes 0.000 description 1
108010043958 Peptoids Proteins 0.000 description 1
241000286209 Phasianidae Species 0.000 description 1
LTQCLFMNABRKSH-UHFFFAOYSA-N Phleomycin Natural products N=1C(C=2SC=C(N=2)C(N)=O)CSC=1CCNC(=O)C(C(O)C)NC(=O)C(C)C(O)C(C)NC(=O)C(C(OC1C(C(O)C(O)C(CO)O1)OC1C(C(OC(N)=O)C(O)C(CO)O1)O)C=1NC=NC=1)NC(=O)C1=NC(C(CC(N)=O)NCC(N)C(N)=O)=NC(N)=C1C LTQCLFMNABRKSH-UHFFFAOYSA-N 0.000 description 1
108010035235 Phleomycins Proteins 0.000 description 1
206010035226 Plasma cell myeloma Diseases 0.000 description 1
108010039918 Polylysine Proteins 0.000 description 1
208000006664 Precursor Cell Lymphoblastic Leukemia-Lymphoma Diseases 0.000 description 1
101710083689 Probable capsid protein Proteins 0.000 description 1
ONIBWKKTOPOVIA-UHFFFAOYSA-N Proline Natural products OC(=O)C1CCCN1 ONIBWKKTOPOVIA-UHFFFAOYSA-N 0.000 description 1
102220472229 Protein Wnt-2_C37S_mutation Human genes 0.000 description 1
102220468734 RING finger and SPRY domain-containing protein 1_C40A_mutation Human genes 0.000 description 1
241000700159 Rattus Species 0.000 description 1
208000035415 Reinfection Diseases 0.000 description 1
108700008625 Reporter Genes Proteins 0.000 description 1
101800000958 Reverse transcriptase/ribonuclease H Proteins 0.000 description 1
102100034344 Ribonuclease H Human genes 0.000 description 1
241000283984 Rodentia Species 0.000 description 1
238000012300 Sequence Analysis Methods 0.000 description 1
108020004682 Single-Stranded DNA Proteins 0.000 description 1
238000002105 Southern blotting Methods 0.000 description 1
241000256251 Spodoptera frugiperda Species 0.000 description 1
108010039445 Stem Cell Factor Proteins 0.000 description 1
241000194017 Streptococcus Species 0.000 description 1
101710172711 Structural protein Proteins 0.000 description 1
108091027544 Subgenomic mRNA Proteins 0.000 description 1
241000282887 Suidae Species 0.000 description 1
QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 1
101710091286 Syncytin-1 Proteins 0.000 description 1
101710091284 Syncytin-2 Proteins 0.000 description 1
108700005078 Synthetic Genes Proteins 0.000 description 1
230000005867 T cell response Effects 0.000 description 1
208000000389 T-cell leukemia Diseases 0.000 description 1
208000028530 T-cell lymphoblastic leukemia/lymphoma Diseases 0.000 description 1
108700026226 TATA Box Proteins 0.000 description 1
206010043376 Tetanus Diseases 0.000 description 1
239000004098 Tetracycline Substances 0.000 description 1
RYYWUUFWQRZTIU-UHFFFAOYSA-N Thiophosphoric acid Chemical class OP(O)(S)=O RYYWUUFWQRZTIU-UHFFFAOYSA-N 0.000 description 1
108091023040 Transcription factor Proteins 0.000 description 1
102000040945 Transcription factor Human genes 0.000 description 1
101710184535 Transmembrane protein Proteins 0.000 description 1
102220554781 Transmembrane protein 116_C22G_mutation Human genes 0.000 description 1
101710141239 Transmembrane protein domain Proteins 0.000 description 1
WGLPBDUCMAPZCE-UHFFFAOYSA-N Trioxochromium Chemical compound O=[Cr](=O)=O WGLPBDUCMAPZCE-UHFFFAOYSA-N 0.000 description 1
101710110267 Truncated transmembrane protein Proteins 0.000 description 1
KZSNJWFQEVHDMF-UHFFFAOYSA-N Valine Natural products CC(C)C(N)C(O)=O KZSNJWFQEVHDMF-UHFFFAOYSA-N 0.000 description 1
241000710959 Venezuelan equine encephalitis virus Species 0.000 description 1
206010058874 Viraemia Diseases 0.000 description 1
230000010530 Virus Neutralization Effects 0.000 description 1
241000269370 Xenopus <genus> Species 0.000 description 1
241000235015 Yarrowia lipolytica Species 0.000 description 1
LUTSRLYCMSCGCS-BWOMAWGNSA-N [(3s,8r,9s,10r,13s)-10,13-dimethyl-17-oxo-1,2,3,4,7,8,9,11,12,16-decahydrocyclopenta[a]phenanthren-3-yl] acetate Chemical compound C([C@@H]12)C[C@]3(C)C(=O)CC=C3[C@@H]1CC=C1[C@]2(C)CC[C@H](OC(=O)C)C1 LUTSRLYCMSCGCS-BWOMAWGNSA-N 0.000 description 1
HMNZFMSWFCAGGW-XPWSMXQVSA-N [3-[hydroxy(2-hydroxyethoxy)phosphoryl]oxy-2-[(e)-octadec-9-enoyl]oxypropyl] (e)-octadec-9-enoate Chemical compound CCCCCCCC\C=C\CCCCCCCC(=O)OCC(COP(O)(=O)OCCO)OC(=O)CCCCCCC\C=C\CCCCCCCC HMNZFMSWFCAGGW-XPWSMXQVSA-N 0.000 description 1
QPMSXSBEVQLBIL-CZRHPSIPSA-N ac1mix0p Chemical compound C1=CC=C2N(C[C@H](C)CN(C)C)C3=CC(OC)=CC=C3SC2=C1.O([C@H]1[C@]2(OC)C=CC34C[C@@H]2[C@](C)(O)CCC)C2=C5[C@]41CCN(C)[C@@H]3CC5=CC=C2O QPMSXSBEVQLBIL-CZRHPSIPSA-N 0.000 description 1
230000002378 acidificating effect Effects 0.000 description 1
229930183665 actinomycin Natural products 0.000 description 1
230000003213 activating effect Effects 0.000 description 1
230000006978 adaptation Effects 0.000 description 1
238000012382 advanced drug delivery Methods 0.000 description 1
239000000556 agonist Substances 0.000 description 1
235000004279 alanine Nutrition 0.000 description 1
AZDRQVAHHNSJOQ-UHFFFAOYSA-N alumane Chemical class [AlH3] AZDRQVAHHNSJOQ-UHFFFAOYSA-N 0.000 description 1
229910052782 aluminium Inorganic materials 0.000 description 1
WNROFYMDJYEPJX-UHFFFAOYSA-K aluminium hydroxide Chemical compound [OH-].[OH-].[OH-].[Al+3] WNROFYMDJYEPJX-UHFFFAOYSA-K 0.000 description 1
ILRRQNADMUWWFW-UHFFFAOYSA-K aluminium phosphate Chemical compound O1[Al]2OP1(=O)O2 ILRRQNADMUWWFW-UHFFFAOYSA-K 0.000 description 1
235000012211 aluminium silicate Nutrition 0.000 description 1
DIZPMCHEQGEION-UHFFFAOYSA-H aluminium sulfate (anhydrous) Chemical compound [Al+3].[Al+3].[O-]S([O-])(=O)=O.[O-]S([O-])(=O)=O.[O-]S([O-])(=O)=O DIZPMCHEQGEION-UHFFFAOYSA-H 0.000 description 1
229940126575 aminoglycoside Drugs 0.000 description 1
229940045799 anthracyclines and related substance Drugs 0.000 description 1
230000003302 anti-idiotype Effects 0.000 description 1
230000030741 antigen processing and presentation Effects 0.000 description 1
229960001230 asparagine Drugs 0.000 description 1
235000009582 asparagine Nutrition 0.000 description 1
229940009098 aspartate Drugs 0.000 description 1
239000012298 atmosphere Substances 0.000 description 1
239000011324 bead Substances 0.000 description 1
230000009286 beneficial effect Effects 0.000 description 1
239000000440 bentonite Substances 0.000 description 1
229910000278 bentonite Inorganic materials 0.000 description 1
SVPXDRXYRYOSEX-UHFFFAOYSA-N bentoquatam Chemical compound O.O=[Si]=O.O=[Al]O[Al]=O SVPXDRXYRYOSEX-UHFFFAOYSA-N 0.000 description 1
RIIWUGSYXOBDMC-UHFFFAOYSA-N benzene-1,2-diamine;hydron;dichloride Chemical compound Cl.Cl.NC1=CC=CC=C1N RIIWUGSYXOBDMC-UHFFFAOYSA-N 0.000 description 1
230000033228 biological regulation Effects 0.000 description 1
229960002685 biotin Drugs 0.000 description 1
235000020958 biotin Nutrition 0.000 description 1
239000011616 biotin Substances 0.000 description 1
230000000740 bleeding effect Effects 0.000 description 1
239000002981 blocking agent Substances 0.000 description 1
210000004369 blood Anatomy 0.000 description 1
239000008280 blood Substances 0.000 description 1
210000000601 blood cell Anatomy 0.000 description 1
210000001185 bone marrow Anatomy 0.000 description 1
210000004271 bone marrow stromal cell Anatomy 0.000 description 1
229950004398 broxuridine Drugs 0.000 description 1
239000000872 buffer Substances 0.000 description 1
229960003150 bupivacaine Drugs 0.000 description 1
238000002619 cancer immunotherapy Methods 0.000 description 1
229940095731 candida albicans Drugs 0.000 description 1
229940041514 candida albicans extract Drugs 0.000 description 1
210000000234 capsid Anatomy 0.000 description 1
150000004657 carbamic acid derivatives Chemical class 0.000 description 1
239000002340 cardiotoxin Substances 0.000 description 1
231100000677 cardiotoxin Toxicity 0.000 description 1
210000004970 cd4 cell Anatomy 0.000 description 1
230000003915 cell function Effects 0.000 description 1
230000007910 cell fusion Effects 0.000 description 1
239000013553 cell monolayer Substances 0.000 description 1
238000005119 centrifugation Methods 0.000 description 1
230000008859 change Effects 0.000 description 1
230000003196 chaotropic effect Effects 0.000 description 1
238000012512 characterization method Methods 0.000 description 1
235000013330 chicken meat Nutrition 0.000 description 1
WIIZWVCIJKGZOK-RKDXNWHRSA-N chloramphenicol Chemical compound ClC(Cl)C(=O)N[C@H](CO)[C@H](O)C1=CC=C([N+]([O-])=O)C=C1 WIIZWVCIJKGZOK-RKDXNWHRSA-N 0.000 description 1
229960005091 chloramphenicol Drugs 0.000 description 1
235000012000 cholesterol Nutrition 0.000 description 1
238000004587 chromatography analysis Methods 0.000 description 1
229910052804 chromium Inorganic materials 0.000 description 1
239000011651 chromium Substances 0.000 description 1
239000013599 cloning vector Substances 0.000 description 1
230000004186 co-expression Effects 0.000 description 1
230000005757 colony formation Effects 0.000 description 1
238000002856 computational phylogenetic analysis Methods 0.000 description 1
230000001276 controlling effect Effects 0.000 description 1
229920001577 copolymer Polymers 0.000 description 1
239000013078 crystal Substances 0.000 description 1
238000012258 culturing Methods 0.000 description 1
229960004397 cyclophosphamide Drugs 0.000 description 1
XUJNEKJLAYXESH-UHFFFAOYSA-N cysteine Natural products SCC(N)C(O)=O XUJNEKJLAYXESH-UHFFFAOYSA-N 0.000 description 1
125000000151 cysteine group Chemical group N[C@@H](CS)C(=O)* 0.000 description 1
229960003067 cystine Drugs 0.000 description 1
238000004163 cytometry Methods 0.000 description 1
230000006378 damage Effects 0.000 description 1
230000007423 decrease Effects 0.000 description 1
238000000432 density-gradient centrifugation Methods 0.000 description 1
238000013461 design Methods 0.000 description 1
229960000633 dextran sulfate Drugs 0.000 description 1
238000010586 diagram Methods 0.000 description 1
UMGXUWVIJIQANV-UHFFFAOYSA-M didecyl(dimethyl)azanium;bromide Chemical compound [Br-].CCCCCCCCCC[N+](C)(C)CCCCCCCCCC UMGXUWVIJIQANV-UHFFFAOYSA-M 0.000 description 1
230000029087 digestion Effects 0.000 description 1
206010013023 diphtheria Diseases 0.000 description 1
LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 description 1
230000006806 disease prevention Effects 0.000 description 1
NAGJZTKCGNOGPW-UHFFFAOYSA-N dithiophosphoric acid Chemical class OP(O)(S)=S NAGJZTKCGNOGPW-UHFFFAOYSA-N 0.000 description 1
230000002222 downregulating effect Effects 0.000 description 1
238000009510 drug design Methods 0.000 description 1
238000010218 electron microscopic analysis Methods 0.000 description 1
238000001493 electron microscopy Methods 0.000 description 1
238000001962 electrophoresis Methods 0.000 description 1
239000003995 emulsifying agent Substances 0.000 description 1
230000002255 enzymatic effect Effects 0.000 description 1
210000002615 epidermis Anatomy 0.000 description 1
210000003743 erythrocyte Anatomy 0.000 description 1
230000000925 erythroid effect Effects 0.000 description 1
210000003013 erythroid precursor cell Anatomy 0.000 description 1
230000001747 exhibiting effect Effects 0.000 description 1
108700014844 flt3 ligand Proteins 0.000 description 1
XRECTZIEBJDKEO-UHFFFAOYSA-N flucytosine Chemical compound NC1=NC(=O)NC=C1F XRECTZIEBJDKEO-UHFFFAOYSA-N 0.000 description 1
229960004413 flucytosine Drugs 0.000 description 1
235000013305 food Nutrition 0.000 description 1
229960002963 ganciclovir Drugs 0.000 description 1
IRSCQMHQWWYFCW-UHFFFAOYSA-N ganciclovir Chemical compound O=C1NC(N)=NC2=C1N=CN2COC(CO)CO IRSCQMHQWWYFCW-UHFFFAOYSA-N 0.000 description 1
239000000499 gel Substances 0.000 description 1
238000001641 gel filtration chromatography Methods 0.000 description 1
238000002523 gelfiltration Methods 0.000 description 1
102000054767 gene variant Human genes 0.000 description 1
230000002068 genetic effect Effects 0.000 description 1
229930195712 glutamate Natural products 0.000 description 1
229960002743 glutamine Drugs 0.000 description 1
150000004676 glycans Chemical class 0.000 description 1
229960002449 glycine Drugs 0.000 description 1
125000003630 glycyl group Chemical group [H]N([H])C([H])([H])C(*)=O 0.000 description 1
239000001963 growth medium Substances 0.000 description 1
239000003721 gunpowder Substances 0.000 description 1
208000002672 hepatitis B Diseases 0.000 description 1
210000003630 histaminocyte Anatomy 0.000 description 1
HNDVDQJCIGZPNO-UHFFFAOYSA-N histidine Natural products OC(=O)C(N)CC1=CN=CN1 HNDVDQJCIGZPNO-UHFFFAOYSA-N 0.000 description 1
239000005556 hormone Substances 0.000 description 1
229940088597 hormone Drugs 0.000 description 1
230000008348 humoral response Effects 0.000 description 1
229920002674 hyaluronan Polymers 0.000 description 1
229960003160 hyaluronic acid Drugs 0.000 description 1
230000008105 immune reaction Effects 0.000 description 1
229940042743 immune sera Drugs 0.000 description 1
230000036039 immunity Effects 0.000 description 1
238000003119 immunoblot Methods 0.000 description 1
230000000984 immunochemical effect Effects 0.000 description 1
230000017555 immunoglobulin mediated immune response Effects 0.000 description 1
239000012678 infectious agent Substances 0.000 description 1
230000001524 infective effect Effects 0.000 description 1
238000002743 insertional mutagenesis Methods 0.000 description 1
230000003993 interaction Effects 0.000 description 1
229940079322 interferon Drugs 0.000 description 1
229940047124 interferons Drugs 0.000 description 1
229940028885 interleukin-4 Drugs 0.000 description 1
229940100602 interleukin-5 Drugs 0.000 description 1
229940047122 interleukins Drugs 0.000 description 1
229960000310 isoleucine Drugs 0.000 description 1
AGPKZVBTJJNPAG-UHFFFAOYSA-N isoleucine Natural products CCC(C)C(N)C(O)=O AGPKZVBTJJNPAG-UHFFFAOYSA-N 0.000 description 1
NLYAJNPCOHFWQQ-UHFFFAOYSA-N kaolin Chemical compound O.O.O=[Al]O[Si](=O)O[Si](=O)O[Al]=O NLYAJNPCOHFWQQ-UHFFFAOYSA-N 0.000 description 1
210000003734 kidney Anatomy 0.000 description 1
210000003292 kidney cell Anatomy 0.000 description 1
239000003446 ligand Substances 0.000 description 1
230000000670 limiting effect Effects 0.000 description 1
239000007788 liquid Substances 0.000 description 1
230000004807 localization Effects 0.000 description 1
210000004072 lung Anatomy 0.000 description 1
210000001165 lymph node Anatomy 0.000 description 1
208000018555 lymphatic system disease Diseases 0.000 description 1
238000002794 lymphocyte assay Methods 0.000 description 1
210000003738 lymphoid progenitor cell Anatomy 0.000 description 1
230000001589 lymphoproliferative effect Effects 0.000 description 1
HCWCAKKEBCNQJP-UHFFFAOYSA-N magnesium orthosilicate Chemical compound [Mg+2].[Mg+2].[O-][Si]([O-])([O-])[O-] HCWCAKKEBCNQJP-UHFFFAOYSA-N 0.000 description 1
239000000391 magnesium silicate Substances 0.000 description 1
229910052919 magnesium silicate Inorganic materials 0.000 description 1
235000019792 magnesium silicate Nutrition 0.000 description 1
238000007885 magnetic separation Methods 0.000 description 1
230000014759 maintenance of location Effects 0.000 description 1
230000007246 mechanism Effects 0.000 description 1
210000003593 megakaryocyte Anatomy 0.000 description 1
229930182817 methionine Natural products 0.000 description 1
108010005942 methionylglycine Proteins 0.000 description 1
YACKEPLHDIMKIO-UHFFFAOYSA-N methylphosphonic acid Chemical compound CP(O)(O)=O YACKEPLHDIMKIO-UHFFFAOYSA-N 0.000 description 1
244000005700 microbiome Species 0.000 description 1
238000000520 microinjection Methods 0.000 description 1
238000007431 microscopic evaluation Methods 0.000 description 1
238000000386 microscopy Methods 0.000 description 1
230000003278 mimic effect Effects 0.000 description 1
239000007758 minimum essential medium Substances 0.000 description 1
ZAHQPTJLOCWVPG-UHFFFAOYSA-N mitoxantrone dihydrochloride Chemical compound Cl.Cl.O=C1C2=C(O)C=CC(O)=C2C(=O)C2=C1C(NCCNCCO)=CC=C2NCCNCCO ZAHQPTJLOCWVPG-UHFFFAOYSA-N 0.000 description 1
241000514897 mixed subtypes Species 0.000 description 1
239000000178 monomer Substances 0.000 description 1
125000004573 morpholin-4-yl group Chemical group N1(CCOCC1)* 0.000 description 1
210000000663 muscle cell Anatomy 0.000 description 1
201000000050 myeloid neoplasm Diseases 0.000 description 1
210000003643 myeloid progenitor cell Anatomy 0.000 description 1
JXTPJDDICSTXJX-UHFFFAOYSA-N n-Triacontane Natural products CCCCCCCCCCCCCCCCCCCCCCCCCCCCCC JXTPJDDICSTXJX-UHFFFAOYSA-N 0.000 description 1
108700004028 nef Genes Proteins 0.000 description 1
210000000440 neutrophil Anatomy 0.000 description 1
238000007899 nucleic acid hybridization Methods 0.000 description 1
239000007764 o/w emulsion Substances 0.000 description 1
238000002515 oligonucleotide synthesis Methods 0.000 description 1
229960005030 other vaccine in atc Drugs 0.000 description 1
239000006179 pH buffering agent Substances 0.000 description 1
239000005022 packaging material Substances 0.000 description 1
230000010412 perfusion Effects 0.000 description 1
210000005259 peripheral blood Anatomy 0.000 description 1
239000011886 peripheral blood Substances 0.000 description 1
210000003819 peripheral blood mononuclear cell Anatomy 0.000 description 1
230000008823 permeabilization Effects 0.000 description 1
WTJKGGKOPKCXLL-RRHRGVEJSA-N phosphatidylcholine Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@H](COP([O-])(=O)OCC[N+](C)(C)C)OC(=O)CCCCCCCC=CCCCCCCCC WTJKGGKOPKCXLL-RRHRGVEJSA-N 0.000 description 1
150000008104 phosphatidylethanolamines Chemical class 0.000 description 1
230000000704 physical effect Effects 0.000 description 1
108010089520 pol Gene Products Proteins 0.000 description 1
229920000724 poly(L-arginine) polymer Polymers 0.000 description 1
229920000747 poly(lactic acid) Polymers 0.000 description 1
229920002401 polyacrylamide Polymers 0.000 description 1
229920000768 polyamine Polymers 0.000 description 1
108010011110 polyarginine Proteins 0.000 description 1
229920000656 polylysine Polymers 0.000 description 1
108010055896 polyornithine Proteins 0.000 description 1
229920002714 polyornithine Polymers 0.000 description 1
210000004896 polypeptide structure Anatomy 0.000 description 1
229920001282 polysaccharide Polymers 0.000 description 1
239000005017 polysaccharide Substances 0.000 description 1
239000013641 positive control Substances 0.000 description 1
230000004481 post-translational protein modification Effects 0.000 description 1
229940024231 poxvirus vaccine Drugs 0.000 description 1
239000002243 precursor Substances 0.000 description 1
230000002265 prevention Effects 0.000 description 1
229940023867 prime-boost vaccine Drugs 0.000 description 1
238000012545 processing Methods 0.000 description 1
229940002612 prodrug Drugs 0.000 description 1
239000000651 prodrug Substances 0.000 description 1
230000035755 proliferation Effects 0.000 description 1
239000001294 propane Substances 0.000 description 1
230000000069 prophylactic effect Effects 0.000 description 1
125000001436 propyl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])[H] 0.000 description 1
108020001580 protein domains Proteins 0.000 description 1
230000009145 protein modification Effects 0.000 description 1
230000007026 protein scission Effects 0.000 description 1
210000001938 protoplast Anatomy 0.000 description 1
230000002685 pulmonary effect Effects 0.000 description 1
239000001397 quillaja saponaria molina bark Substances 0.000 description 1
230000009257 reactivity Effects 0.000 description 1
230000006798 recombination Effects 0.000 description 1
230000000717 retained effect Effects 0.000 description 1
108700004030 rev Genes Proteins 0.000 description 1
102220267762 rs1340506651 Human genes 0.000 description 1
102200052965 rs35573508 Human genes 0.000 description 1
102220205737 rs771543236 Human genes 0.000 description 1
229930182490 saponin Natural products 0.000 description 1
150000007949 saponins Chemical class 0.000 description 1
238000010845 search algorithm Methods 0.000 description 1
239000006152 selective media Substances 0.000 description 1
230000035945 sensitivity Effects 0.000 description 1
239000002356 single layer Substances 0.000 description 1
238000001542 size-exclusion chromatography Methods 0.000 description 1
210000003491 skin Anatomy 0.000 description 1
238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 description 1
229940063673 spermidine Drugs 0.000 description 1
229940063675 spermine Drugs 0.000 description 1
210000004989 spleen cell Anatomy 0.000 description 1
210000004988 splenocyte Anatomy 0.000 description 1
229940032094 squalane Drugs 0.000 description 1
238000010186 staining Methods 0.000 description 1
108010042747 stallimycin Proteins 0.000 description 1
229950009902 stallimycin Drugs 0.000 description 1
230000004936 stimulating effect Effects 0.000 description 1
230000000638 stimulation Effects 0.000 description 1
239000000758 substrate Substances 0.000 description 1
229940124530 sulfonamide Drugs 0.000 description 1
150000003456 sulfonamides Chemical class 0.000 description 1
235000011149 sulphuric acid Nutrition 0.000 description 1
239000000829 suppository Substances 0.000 description 1
238000004114 suspension culture Methods 0.000 description 1
239000000454 talc Substances 0.000 description 1
229910052623 talc Inorganic materials 0.000 description 1
108700004027 tat Genes Proteins 0.000 description 1
229960002180 tetracycline Drugs 0.000 description 1
229930101283 tetracycline Natural products 0.000 description 1
235000019364 tetracycline Nutrition 0.000 description 1
150000003522 tetracyclines Chemical class 0.000 description 1
210000001541 thymus gland Anatomy 0.000 description 1
239000000724 thymus hormone Substances 0.000 description 1
238000012549 training Methods 0.000 description 1
230000002103 transcriptional effect Effects 0.000 description 1
230000001131 transforming effect Effects 0.000 description 1
238000003146 transient transfection Methods 0.000 description 1
102000035160 transmembrane proteins Human genes 0.000 description 1
108091005703 transmembrane proteins Proteins 0.000 description 1
230000032258 transport Effects 0.000 description 1
230000001960 triggered effect Effects 0.000 description 1
239000013638 trimer Substances 0.000 description 1
JOPDZQBPOWAEHC-UHFFFAOYSA-H tristrontium;diphosphate Chemical compound [Sr+2].[Sr+2].[Sr+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O JOPDZQBPOWAEHC-UHFFFAOYSA-H 0.000 description 1
239000004474 valine Substances 0.000 description 1
229920002554 vinyl polymer Polymers 0.000 description 1
230000009447 viral pathogenesis Effects 0.000 description 1
230000006656 viral protein synthesis Effects 0.000 description 1
230000017613 viral reproduction Effects 0.000 description 1
238000005406 washing Methods 0.000 description 1
XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
238000009736 wetting Methods 0.000 description 1
239000000080 wetting agent Substances 0.000 description 1
238000002424 x-ray crystallography Methods 0.000 description 1
210000005253 yeast cell Anatomy 0.000 description 1
239000012138 yeast extract Substances 0.000 description 1

Landscapes

Peptides Or Proteins (AREA)
Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)
Medicines Containing Material From Animals Or Micro-Organisms (AREA)

CA2785699A 2001-07-05 2002-07-05 Polynucleotides codant des polypeptides de type c du vih antigeniques, polypeptides et leurs utilisations Abandoned CA2785699A1 (fr)

Applications Claiming Priority (7)

Application Number	Priority Date	Filing Date	Title
US30319201P	2001-07-05	2001-07-05
US60/303,192		2001-07-05
US31686001P	2001-08-31	2001-08-31
US60/316,860		2001-08-31
US34987102P	2002-01-16	2002-01-16
US60/349,871		2002-01-16
CA2634992A CA2634992C (fr)	2001-07-05	2002-07-05	Polynucleotides codant des polypeptides de type c du vih antigeniques, polypeptides et leurs utilisations

Related Parent Applications (1)

Application Number	Title	Priority Date	Filing Date
CA2634992A Division CA2634992C (fr)	2001-07-05	2002-07-05	Polynucleotides codant des polypeptides de type c du vih antigeniques, polypeptides et leurs utilisations

Publications (1)

Publication Number	Publication Date
CA2785699A1 true CA2785699A1 (fr)	2003-01-16

Family

ID=35160894

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
CA2785699A Abandoned CA2785699A1 (fr)	2001-07-05	2002-07-05	Polynucleotides codant des polypeptides de type c du vih antigeniques, polypeptides et leurs utilisations

Country Status (2)

Country	Link
CA (1)	CA2785699A1 (fr)
ZA (1)	ZA200400793B (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
CN114686495A (zh) *	2015-12-15	2022-07-01	扬森疫苗与预防公司	人类免疫缺陷病毒抗原、载体、组合物、及其使用方法

2002
- 2002-07-05 CA CA2785699A patent/CA2785699A1/fr not_active Abandoned
2004
- 2004-01-30 ZA ZA200400793A patent/ZA200400793B/en unknown

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
CN114686495A (zh) *	2015-12-15	2022-07-01	扬森疫苗与预防公司	人类免疫缺陷病毒抗原、载体、组合物、及其使用方法

Also Published As

Publication number	Publication date
ZA200400793B (en)	2006-02-22

Publication	Publication Date	Title
US9598469B2 (en)	2017-03-21	HIV-1 south african subtype C env proteins
US8263394B2 (en)	2012-09-11	Polynucleotides encoding antigenic HIV type B polypeptides, polypeptides, and uses thereof
CA2414374C (fr)	2014-01-21	Polynucleotides codant pour des polypeptides antigeniques du type c du vih, de tels polypeptides et leurs utilisations
US20080095833A1 (en)	2008-04-24	Polynucleotides encoding antigenic hiv type b polypeptides, polypeptides, and uses thereof
WO2000039304A9 (fr)	2000-12-07	Polynucleotides codant pour des polypeptides antigeniques du type c du vih; polypeptides et leurs utilisations
US20030198621A1 (en)	2003-10-23	Polynucleotides encoding antigenic HIV type B and/or type C polypeptides, polypeptides and uses thereof
CA2785699A1 (fr)	2003-01-16	Polynucleotides codant des polypeptides de type c du vih antigeniques, polypeptides et leurs utilisations
EP1535995A1 (fr)	2005-06-01	Polynucléotides codant pour le polypeptide antigénique env du virus VIH de type C et leurs utilisations

Legal Events

Date	Code	Title	Description
2012-08-09	EEER	Examination request
2015-03-15	FZDE	Dead	Effective date: 20150116

CA2785699A1 - Polynucleotides codant des polypeptides de type c du vih antigeniques, polypeptides et leurs utilisations - Google Patents

Info

Links

Landscapes

Applications Claiming Priority (7)

Related Parent Applications (1)

Publications (1)

Family

ID=35160894

Family Applications (1)

Country Status (2)

Cited By (1)

Cited By (1)

Also Published As

Similar Documents

Legal Events