CA2740150A1 - Device, kit and method for pulsing biological samples with an agent and stabilising the sample so pulsed - Google Patents
Device, kit and method for pulsing biological samples with an agent and stabilising the sample so pulsed Download PDFInfo
- Publication number
- CA2740150A1 CA2740150A1 CA2740150A CA2740150A CA2740150A1 CA 2740150 A1 CA2740150 A1 CA 2740150A1 CA 2740150 A CA2740150 A CA 2740150A CA 2740150 A CA2740150 A CA 2740150A CA 2740150 A1 CA2740150 A1 CA 2740150A1
- Authority
- CA
- Canada
- Prior art keywords
- vessel
- kit according
- substance
- control
- antigens
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
- B01L3/5082—Test tubes per se
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/56—Labware specially adapted for transferring fluids
- B01L3/563—Joints or fittings ; Separable fluid transfer means to transfer fluids between at least two containers, e.g. connectors
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/02—Adapting objects or devices to another
- B01L2200/026—Fluid interfacing between devices or objects, e.g. connectors, inlet details
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/04—Closures and closing means
- B01L2300/041—Connecting closures to device or container
- B01L2300/042—Caps; Plugs
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/04—Closures and closing means
- B01L2300/041—Connecting closures to device or container
- B01L2300/044—Connecting closures to device or container pierceable, e.g. films, membranes
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/04—Closures and closing means
- B01L2300/046—Function or devices integrated in the closure
- B01L2300/047—Additional chamber, reservoir
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/04—Closures and closing means
- B01L2300/046—Function or devices integrated in the closure
- B01L2300/048—Function or devices integrated in the closure enabling gas exchange, e.g. vents
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0848—Specific forms of parts of containers
- B01L2300/0858—Side walls
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
- B01L2400/0478—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure pistons
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
- B01L2400/0481—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure squeezing of channels or chambers
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
- B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Clinical Laboratory Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Hematology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The present invention is related to a device and kitfor pulsing a biological sample with a pulsing agent, subsequently stabilizing the biolog-ical sample so pulsed, which device or kit provides a control reaction. The invention has application in the fields of medical diagnostics, particularly re-lating to immunity.
Claims (37)
1. An assay device (30) for a liquid biological sample, which facilitates exposure of said sample separately to a first substance (2) and a control substance (35), and subsequently to a nucleic acid stabilizing agent (5), the device comprising:
- a first compartment (32) in which said first substance (2) is present, - a second compartment (34) in which said control substance (35) is present, - a third compartment (36) where said stabilizing agent (5) is present, and - a support (52) for a biological sample tube, wherein one or more of the compartments (32, 34, 36) is sealed and comprises one or more areas suitable for puncture by a hollow needle.
- a first compartment (32) in which said first substance (2) is present, - a second compartment (34) in which said control substance (35) is present, - a third compartment (36) where said stabilizing agent (5) is present, and - a support (52) for a biological sample tube, wherein one or more of the compartments (32, 34, 36) is sealed and comprises one or more areas suitable for puncture by a hollow needle.
2. Device according to claim 1, wherein said area is a re-sealable septum (40, 42, 46).
3. Device according to claim 1 or 2, further comprising a support (54, 56) for demountable attachment to a transfer-tubing (60), which transfer tubing comprises a hollow flexible or rigid tubing (64) disposed with a hollow needle (62, 66) at either end, adapted for transfer of liquid between any two compartments, or between a compartment and the biological sample tube.
4. Device according to claim 3, further comprising the transfer tubing (60) as defined in claim 3.
5. Device according to claim 3 or 4, wherein one needle (62) of the transfer tubing is attached in parallel alignment to the hollow needle (80) of a pressurising tubing (88), which pressurising tubing comprises a flexible hollow tubing (82) disposed at one end with said needle (80), and is adapted to apply vacuum or pressure to a compartment or biological sample tube to propel liquid through the transfer tubing.
6. Device according to any of claims 1 to 5, wherein said first substance (2) is immobilised on part or all of the inside surface of said first compartment (32).
7. Device according to any of claims 1 to 6, wherein one or more of the compartments (32, 34, 36) comprises an air vent.
8. Device according to any of claims 1 to 7, wherein the first (32) and/or second (34) compartments are held under negative pressure.
9. Device according to any of claims 1 to 8, wherein the first (32) and/or second (34) compartments comprise an indication for dispensing a known volume of stabilising agent therein.
10. Device according to any of claims 1 to 9, wherein said first substance (5) comprises one or more immune system antigens.
11. Device according to claim 10 wherein said immune system antigens are vaccine components.
12. Device according to claim 10 wherein said immune system antigens are antigens which provoke a hyperallergenic response.
13. Device according to claim 10 wherein said immune system antigens are one or more selected from histocompatibility antigens, bacterial LPS, tetanous toxoid, a cancer immunotherapy antigen, MAGE-3, a cat allergen, Feld1, antigen presenting cells from an organ donor, an autoantigen, GAD65.
14. Device according to any of claims 1 to 13 wherein said stabilizing agent (35) is an inhibitor of cellular RNA degradation and/or gene induction.
15. Device according to claim 14 wherein said inhibitor of cellular RNA
degradation and/or gene induction is that as found in a PAXgene.TM. or Tempus .TM. Blood RNA Tube.
degradation and/or gene induction is that as found in a PAXgene.TM. or Tempus .TM. Blood RNA Tube.
16. A kit for assaying a liquid biological sample comprising:
a vessel (1) suitable for accepting liquid biological sample, exposing said sample to a first substance (2) and subsequently a nucleic acid stabilising agent, said vessel comprising:
a) a first substance (2) present inside said vessel (1), b) a container (4, 12, 16) in which said stabilising agent (5) is present, c) a connection between the inside of said vessel (1) and the inside of said container (4, 12, 16), d) a physical barrier (25, 15, 18) that temporarily blocks said connection;
and a control vessel (37, 37') suitable for accepting liquid biological sample, exposing said sample to a control substance and subsequently a nucleic acid stabilising agent, said control vessel comprising:
a) a control substance (35) present inside said control vessel (37, 37'), b) a control container (4', 12', 16') in which said stabilising agent (5') is present, c) a connection between the inside of said control vessel (37, 37') and the inside of said control container (4', 12', 16'), d) a physical barrier (15', 18', 25) that temporarily blocks said connection, wherein the vessel (1) and/or control vessel (37, 37') comprise one or more areas suitable for puncture by a syringe needle.
a vessel (1) suitable for accepting liquid biological sample, exposing said sample to a first substance (2) and subsequently a nucleic acid stabilising agent, said vessel comprising:
a) a first substance (2) present inside said vessel (1), b) a container (4, 12, 16) in which said stabilising agent (5) is present, c) a connection between the inside of said vessel (1) and the inside of said container (4, 12, 16), d) a physical barrier (25, 15, 18) that temporarily blocks said connection;
and a control vessel (37, 37') suitable for accepting liquid biological sample, exposing said sample to a control substance and subsequently a nucleic acid stabilising agent, said control vessel comprising:
a) a control substance (35) present inside said control vessel (37, 37'), b) a control container (4', 12', 16') in which said stabilising agent (5') is present, c) a connection between the inside of said control vessel (37, 37') and the inside of said control container (4', 12', 16'), d) a physical barrier (15', 18', 25) that temporarily blocks said connection, wherein the vessel (1) and/or control vessel (37, 37') comprise one or more areas suitable for puncture by a syringe needle.
17. Kit according to claim 16 wherein said first substance (2) is immobilised on part or all of the inside surface of said vessel (1).
18. Kit according to claim 16 wherein said first substance (2) is immobilised on a solid support.
19. Kit according to any of claims 16 to 18 wherein said first substance (2) is a liquid.
20. Kit according to any of claims 16 to 18 wherein said first substance (2) is a solid.
21. Kit according to any of claims 16 to 20 wherein said area is a re-sealable septum.
22. Kit according to any of claims 16 to 21, wherein the vessel (1) and/or control vessel (37, 37') comprise a fitting suitable for receiving a syringe and transmitting the contents therein to the interior of said vessel (1) or control vessel (37, 37').
23. Kit according to any of claims 16 to 22, wherein the vessel (1) and/or control vessel (37, 37') comprise a fitting suitable for receiving a syringe needle.
24. Kit according to any of claims 16 to 23, wherein the vessel (1) and/or control vessel (37, 37') comprise a valve which is capable of minimising the flow of gas/liquid from vessel, and allowing the flow of liquid biological sample into the vessel.
25. Kit according to any of claims 16 to 24, wherein the vessel (1) and/or control vessel (37, 37') comprise a means through which displaced gas may be expelled.
26. Kit according to any of claims 16 to 25, wherein the vessel (1) and/or control vessel (37, 37') are held under negative pressure.
27. Kit according to any of claims 16 to 26, wherein the physical barrier (15, 18, 25, 15', 18', 25') of item d) is opened by the application of physical force to said vessel or control vessel.
28. Kit according claim 27, wherein said force transmits an opening means to said physical barrier (15, 18, 25, 15', 18', 25').
29. Kit according to claim 27 wherein said force irreversibly opens said physical barrier (15, 18, 25, 15', 18', 25').
30. Kit according to any of claims 16 to 29, wherein said vessel (1) and/or control vessel (37, 37') comprises an indication for dispensing a known volume of stabilising agent therein.
31. Kit according to any of claims 16 to 30, wherein said first substance (2) comprises one or more immune system antigens.
32. Kit according to claim 30 wherein said immune system antigens are vaccine components.
33. Kit according to claim 30 wherein said immune system antigens are antigens which provoke a hyperallergenic response.
34. Kit according to claim 30 wherein said immune system antigens are one or more selected from histocompatibility antigens, bacterial LPS, tetanous toxoid, a cancer immunotherapy antigen, MAGE-3, a cat allergen, Feld1, antigen presenting cells from an organ donor, an autoantigen, and GAD65.
35. Kit according to any of claims 16 to 34, wherein said stabilising agent is an inhibitor of cellular RNA degradation and/or gene induction.
36. Kit according to any of claims 16 to 35, wherein said inhibitor of cellular RNA
degradation and/or gene induction is that as found in a PAXgene.TM. or Tempus .TM. Blood RNA Tube.
degradation and/or gene induction is that as found in a PAXgene.TM. or Tempus .TM. Blood RNA Tube.
37. Kit according to any of claims 16 to 36, wherein the exterior of the vessel (1) and control vessel (37, 37') are joined to form a single entity.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PCT/EP2008/064030 WO2010043271A1 (en) | 2008-10-17 | 2008-10-17 | Device, kit and method for pulsing biological samples with an agent and stabilising the sample so pulsed |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2740150A1 true CA2740150A1 (en) | 2010-04-22 |
| CA2740150C CA2740150C (en) | 2012-09-25 |
Family
ID=40344582
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2740150A Expired - Fee Related CA2740150C (en) | 2008-10-17 | 2008-10-17 | Device, kit and method for pulsing biological samples with an agent and stabilising the sample so pulsed |
Country Status (6)
| Country | Link |
|---|---|
| EP (1) | EP2346606A1 (en) |
| JP (1) | JP2012505639A (en) |
| CN (1) | CN102186590A (en) |
| CA (1) | CA2740150C (en) |
| IL (1) | IL212082A0 (en) |
| WO (1) | WO2010043271A1 (en) |
Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2793554C (en) | 2010-03-19 | 2018-04-03 | Atomo Diagnostics Pty Limited | Bodily fluid diagnostic system |
| AU2011316495B2 (en) | 2010-10-15 | 2014-06-12 | Atomo Diagnostics Limited | Sampling assembly |
| WO2015075677A1 (en) | 2013-11-21 | 2015-05-28 | Atomo Diagnostics Pty Limited | Fluid control in integrated testing devices |
| EP2959971A1 (en) * | 2014-06-27 | 2015-12-30 | Euroimmun Medizinische Labordiagnostika AG | Method and device for the transfer of liquids |
| BR112017007237B1 (en) * | 2014-10-15 | 2020-12-01 | Merck Patent Gmbh | sample preparation container |
| CN105039548A (en) * | 2015-07-28 | 2015-11-11 | 深圳爱生再生医学科技有限公司 | IL-4 detection primer, kit and semi-quantitative detection method |
| MA50571A (en) * | 2017-11-10 | 2020-09-16 | Juno Therapeutics Inc | CLOSED SYSTEM CRYOGENIC TANKS |
| CN110012899A (en) * | 2019-04-12 | 2019-07-16 | 南京科佰生物科技有限公司 | RNA stabilizer for cells and method of using the same |
| CN110176165B (en) * | 2019-06-20 | 2022-02-15 | 中国石油大学(华东) | A comprehensive experimental method for thermal simulation of "hydrocarbon-water-rock" interaction |
| CN116519968B (en) * | 2023-06-25 | 2023-09-08 | 成都云芯医联科技有限公司 | Integrated multi-reagent sample mixing and adding device |
Family Cites Families (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100535381B1 (en) * | 1997-09-16 | 2005-12-09 | 세키스이가가쿠 고교가부시키가이샤 | Blood Test Container and Blood Test Method |
| JP3551293B2 (en) * | 1998-02-02 | 2004-08-04 | 東洋紡績株式会社 | Nucleic acid extraction device |
| CA2428864C (en) * | 2000-11-08 | 2011-04-12 | Becton, Dickinson And Company | Method and device for collecting and stabilizing a biological sample |
| US20040256331A1 (en) * | 2002-10-04 | 2004-12-23 | Arking E. James | System and method for fractionation of a centrifuged sample |
| US7700276B2 (en) * | 2003-07-10 | 2010-04-20 | Universite Libre De Bruxelles | Device, kit and method for pulsing biological samples with an agent and stabilising the sample so pulsed |
| ES2347564T3 (en) * | 2003-08-05 | 2010-11-02 | Becton, Dickinson And Company | DEVICE AND METHODS FOR THE COLLECTION OF A SAMPLE OF BIOLOGICAL FLUID AND THE TREATMENT OF SELECTED COMPONENTS. |
| EP1611954A1 (en) * | 2004-07-03 | 2006-01-04 | Roche Diagnostics GmbH | Liquid reservoir connector |
| EP1614464A1 (en) * | 2004-07-03 | 2006-01-11 | Roche Diagnostics GmbH | Liquid reservoir connector |
| US20070140915A1 (en) * | 2005-12-12 | 2007-06-21 | Cytyc Corporation | Method and Apparatus for Obtaining Aliquot from Liquid-Based Cytological Sample |
| JP2009540868A (en) * | 2006-06-15 | 2009-11-26 | ストラタジーン カリフォルニア | System for isolating biomolecules from samples |
| US20080153078A1 (en) * | 2006-06-15 | 2008-06-26 | Braman Jeffrey C | System for isolating biomolecules from a sample |
| US7674434B2 (en) * | 2006-11-27 | 2010-03-09 | Cytyc Corporation | Vials and apparatus for obtaining an aliquot of a sample |
| WO2008137008A2 (en) * | 2007-05-04 | 2008-11-13 | Claros Diagnostics, Inc. | Fluidic connectors and microfluidic systems |
-
2008
- 2008-10-17 CA CA2740150A patent/CA2740150C/en not_active Expired - Fee Related
- 2008-10-17 JP JP2011531366A patent/JP2012505639A/en not_active Ceased
- 2008-10-17 CN CN2008801316095A patent/CN102186590A/en active Pending
- 2008-10-17 WO PCT/EP2008/064030 patent/WO2010043271A1/en active Application Filing
- 2008-10-17 EP EP08875202A patent/EP2346606A1/en not_active Withdrawn
-
2011
- 2011-04-03 IL IL212082A patent/IL212082A0/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| CN102186590A (en) | 2011-09-14 |
| IL212082A0 (en) | 2011-06-30 |
| EP2346606A1 (en) | 2011-07-27 |
| JP2012505639A (en) | 2012-03-08 |
| CA2740150C (en) | 2012-09-25 |
| WO2010043271A1 (en) | 2010-04-22 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request | ||
| MKLA | Lapsed |
Effective date: 20141017 |