CA2410688A1 - A system for cell-based screening - Google Patents
A system for cell-based screening Download PDFInfo
- Publication number
- CA2410688A1 CA2410688A1 CA002410688A CA2410688A CA2410688A1 CA 2410688 A1 CA2410688 A1 CA 2410688A1 CA 002410688 A CA002410688 A CA 002410688A CA 2410688 A CA2410688 A CA 2410688A CA 2410688 A1 CA2410688 A1 CA 2410688A1
- Authority
- CA
- Canada
- Prior art keywords
- cells
- locations
- array
- reporter molecules
- microplate
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000012216 screening Methods 0.000 title abstract 2
- 230000003287 optical effect Effects 0.000 abstract 3
- 230000000694 effects Effects 0.000 abstract 2
- 238000000034 method Methods 0.000 abstract 2
- 230000008827 biological function Effects 0.000 abstract 1
- 150000001875 compounds Chemical class 0.000 abstract 1
Landscapes
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The present invention provides systems, methods, and screens for an optical system analysis of cells to rapidly determine the distribution, environment, or activity of fluorescently labeled reporter molecules in cells for the purpose of screening large numbers of compounds for those that specifically affect particular biological functions. The invention involves providing cells containing fluorescent reporter molecules in an array of locations and scanning numerous cells in each location with a high magnification fluorescence optical system, converting the optical information into digital data, and utilizing the digital data to determine the distribution, environment or activity of the fluorescently labeled reporter molecules in the cells. The array of locations may be an industry standard 96 well or 384 well microtiter plate or a microplate which is microplate having cells in a micropaterned array of locations. The invention includes apparatus and computerized method for processing, displaying and storing the data.
Applications Claiming Priority (11)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US08/810,983 | 1997-02-27 | ||
| US08/810,983 US5989835A (en) | 1997-02-27 | 1997-02-27 | System for cell-based screening |
| WOPCT/US97/09564 | 1997-05-29 | ||
| US08/865,341 US6103479A (en) | 1996-05-30 | 1997-05-29 | Miniaturized cell array methods and apparatus for cell-based screening |
| US08/865,341 | 1997-05-29 | ||
| PCT/US1997/009564 WO1997045730A1 (en) | 1996-05-30 | 1997-05-29 | Miniaturized cell array methods and apparatus for cell-based screening |
| US6924697P | 1997-12-11 | 1997-12-11 | |
| US6932997P | 1997-12-11 | 1997-12-11 | |
| US60/069,329 | 1997-12-11 | ||
| US60/069,246 | 1997-12-11 | ||
| CA002282658A CA2282658C (en) | 1997-02-27 | 1998-02-27 | A system for cell-based screening |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002282658A Division CA2282658C (en) | 1997-02-27 | 1998-02-27 | A system for cell-based screening |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2410688A1 true CA2410688A1 (en) | 1998-09-03 |
| CA2410688C CA2410688C (en) | 2004-10-26 |
Family
ID=33425598
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002410688A Expired - Lifetime CA2410688C (en) | 1997-02-27 | 1998-02-27 | A system for cell-based screening |
Country Status (1)
| Country | Link |
|---|---|
| CA (1) | CA2410688C (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3093649A3 (en) * | 1998-05-16 | 2017-04-26 | Life Technologies Corporation | An optical instrument monitoring dna polymerase chain reactions |
| US9823195B2 (en) | 1998-05-16 | 2017-11-21 | Life Technologies Corporation | Optical instrument comprising multi-notch beam splitter |
| WO2023226884A1 (en) * | 2022-05-24 | 2023-11-30 | Bioarchitec Group Limited | High-throughput selection and fabrication of biomaterial-encapsulated cell mass and uses thereof |
-
1998
- 1998-02-27 CA CA002410688A patent/CA2410688C/en not_active Expired - Lifetime
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3093649A3 (en) * | 1998-05-16 | 2017-04-26 | Life Technologies Corporation | An optical instrument monitoring dna polymerase chain reactions |
| US9671342B2 (en) | 1998-05-16 | 2017-06-06 | Life Technologies Corporation | Instrument for monitoring polymerase chain reaction of DNA |
| US9823195B2 (en) | 1998-05-16 | 2017-11-21 | Life Technologies Corporation | Optical instrument comprising multi-notch beam splitter |
| WO2023226884A1 (en) * | 2022-05-24 | 2023-11-30 | Bioarchitec Group Limited | High-throughput selection and fabrication of biomaterial-encapsulated cell mass and uses thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CA2410688C (en) | 2004-10-26 |
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| Sessions | Conferences and meetings |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request | ||
| MKEX | Expiry |
Effective date: 20180227 |