CA2381204A1 - Detection et quantification basees sur l'amplification en chaine par polymerase de tapesia yallundae et tapesia acuformis - Google Patents
Detection et quantification basees sur l'amplification en chaine par polymerase de tapesia yallundae et tapesia acuformis Download PDFInfo
- Publication number
- CA2381204A1 CA2381204A1 CA002381204A CA2381204A CA2381204A1 CA 2381204 A1 CA2381204 A1 CA 2381204A1 CA 002381204 A CA002381204 A CA 002381204A CA 2381204 A CA2381204 A CA 2381204A CA 2381204 A1 CA2381204 A1 CA 2381204A1
- Authority
- CA
- Canada
- Prior art keywords
- seq
- dna
- tapesia
- primer
- pair
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
- 241000144610 Oculimacula acuformis Species 0.000 title claims abstract description 61
- 241001668536 Oculimacula yallundae Species 0.000 title claims abstract description 54
- 238000001514 detection method Methods 0.000 title claims abstract description 27
- 238000011002 quantification Methods 0.000 title description 11
- 241000209140 Triticum Species 0.000 claims abstract description 100
- 235000021307 Triticum Nutrition 0.000 claims abstract description 99
- 239000000523 sample Substances 0.000 claims abstract description 83
- 239000013615 primer Substances 0.000 claims description 127
- 108020004414 DNA Proteins 0.000 claims description 109
- 108091023242 Internal transcribed spacer Proteins 0.000 claims description 56
- 239000002773 nucleotide Substances 0.000 claims description 45
- 125000003729 nucleotide group Chemical group 0.000 claims description 44
- 244000052769 pathogen Species 0.000 claims description 42
- 238000003752 polymerase chain reaction Methods 0.000 claims description 33
- 244000053095 fungal pathogen Species 0.000 claims description 30
- 238000000034 method Methods 0.000 claims description 30
- 230000002538 fungal effect Effects 0.000 claims description 28
- 230000001717 pathogenic effect Effects 0.000 claims description 26
- 241000196324 Embryophyta Species 0.000 claims description 17
- 239000003155 DNA primer Substances 0.000 claims description 16
- 108091034117 Oligonucleotide Proteins 0.000 claims description 14
- 230000003321 amplification Effects 0.000 claims description 13
- 238000003199 nucleic acid amplification method Methods 0.000 claims description 13
- 108020000949 Fungal DNA Proteins 0.000 claims description 12
- 239000002751 oligonucleotide probe Substances 0.000 claims description 12
- 108020005187 Oligonucleotide Probes Proteins 0.000 claims description 10
- 238000012408 PCR amplification Methods 0.000 claims description 10
- 230000000295 complement effect Effects 0.000 claims description 8
- 238000009007 Diagnostic Kit Methods 0.000 claims description 3
- 238000003556 assay Methods 0.000 abstract description 55
- 238000003753 real-time PCR Methods 0.000 abstract description 6
- 241001668538 Mollisia Species 0.000 description 60
- 239000002987 primer (paints) Substances 0.000 description 26
- 238000006243 chemical reaction Methods 0.000 description 23
- 210000001519 tissue Anatomy 0.000 description 20
- 108091028043 Nucleic acid sequence Proteins 0.000 description 16
- 201000010099 disease Diseases 0.000 description 14
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 14
- 241000894007 species Species 0.000 description 14
- 239000000284 extract Substances 0.000 description 13
- 235000013339 cereals Nutrition 0.000 description 10
- 238000000605 extraction Methods 0.000 description 10
- 239000000417 fungicide Substances 0.000 description 10
- 108700022487 rRNA Genes Proteins 0.000 description 10
- 108090000623 proteins and genes Proteins 0.000 description 9
- 238000012360 testing method Methods 0.000 description 9
- 239000000047 product Substances 0.000 description 8
- 108020004998 Chloroplast DNA Proteins 0.000 description 7
- 230000008685 targeting Effects 0.000 description 7
- 238000005382 thermal cycling Methods 0.000 description 7
- 241000233866 Fungi Species 0.000 description 6
- 238000002869 basic local alignment search tool Methods 0.000 description 6
- 239000000975 dye Substances 0.000 description 6
- 239000012634 fragment Substances 0.000 description 6
- 238000012163 sequencing technique Methods 0.000 description 6
- 108091026890 Coding region Proteins 0.000 description 5
- 241000682843 Pseudocercosporella Species 0.000 description 5
- 108010056141 cytochrome b559 Proteins 0.000 description 5
- 108020004565 5.8S Ribosomal RNA Proteins 0.000 description 4
- 238000007400 DNA extraction Methods 0.000 description 4
- 238000002944 PCR assay Methods 0.000 description 4
- 241001533598 Septoria Species 0.000 description 4
- 230000009260 cross reactivity Effects 0.000 description 4
- 238000011161 development Methods 0.000 description 4
- 230000000855 fungicidal effect Effects 0.000 description 4
- 239000000203 mixture Substances 0.000 description 4
- 239000001965 potato dextrose agar Substances 0.000 description 4
- ABZLKHKQJHEPAX-UHFFFAOYSA-N tetramethylrhodamine Chemical compound C=12C=CC(N(C)C)=CC2=[O+]C2=CC(N(C)C)=CC=C2C=1C1=CC=CC=C1C([O-])=O ABZLKHKQJHEPAX-UHFFFAOYSA-N 0.000 description 4
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 3
- 108090000790 Enzymes Proteins 0.000 description 3
- 102000004190 Enzymes Human genes 0.000 description 3
- 241000736122 Parastagonospora nodorum Species 0.000 description 3
- 108020001027 Ribosomal DNA Proteins 0.000 description 3
- 108010006785 Taq Polymerase Proteins 0.000 description 3
- 241001360088 Zymoseptoria tritici Species 0.000 description 3
- 238000000137 annealing Methods 0.000 description 3
- 239000000872 buffer Substances 0.000 description 3
- 239000003153 chemical reaction reagent Substances 0.000 description 3
- 244000000003 plant pathogen Species 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 230000004044 response Effects 0.000 description 3
- 125000006850 spacer group Chemical group 0.000 description 3
- 208000024891 symptom Diseases 0.000 description 3
- 238000011282 treatment Methods 0.000 description 3
- 108020004463 18S ribosomal RNA Proteins 0.000 description 2
- 108091093088 Amplicon Proteins 0.000 description 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 241000190150 Bipolaris sorokiniana Species 0.000 description 2
- 241001290235 Ceratobasidium cereale Species 0.000 description 2
- 108020001019 DNA Primers Proteins 0.000 description 2
- 241000223194 Fusarium culmorum Species 0.000 description 2
- 241000223195 Fusarium graminearum Species 0.000 description 2
- 241001489200 Fusarium poae Species 0.000 description 2
- 241000233732 Fusarium verticillioides Species 0.000 description 2
- 241000331248 Helgardia aestiva Species 0.000 description 2
- 240000005979 Hordeum vulgare Species 0.000 description 2
- 235000007340 Hordeum vulgare Nutrition 0.000 description 2
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 2
- 241001555224 Microdochium majus Species 0.000 description 2
- 241001459558 Monographella nivalis Species 0.000 description 2
- 241000533218 Phaeosphaeria avenaria f. sp. tritici Species 0.000 description 2
- 241000087479 Pseudocercospora fijiensis Species 0.000 description 2
- 235000007238 Secale cereale Nutrition 0.000 description 2
- 241001597349 Septoria glycines Species 0.000 description 2
- 241000700584 Simplexvirus Species 0.000 description 2
- PXIPVTKHYLBLMZ-UHFFFAOYSA-N Sodium azide Chemical compound [Na+].[N-]=[N+]=[N-] PXIPVTKHYLBLMZ-UHFFFAOYSA-N 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 239000005846 Triadimenol Substances 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 210000004027 cell Anatomy 0.000 description 2
- 239000013599 cloning vector Substances 0.000 description 2
- 238000004590 computer program Methods 0.000 description 2
- 108091036078 conserved sequence Proteins 0.000 description 2
- 238000010790 dilution Methods 0.000 description 2
- 239000012895 dilution Substances 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000002866 fluorescence resonance energy transfer Methods 0.000 description 2
- 244000000004 fungal plant pathogen Species 0.000 description 2
- 230000005764 inhibitory process Effects 0.000 description 2
- 238000002955 isolation Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 238000002844 melting Methods 0.000 description 2
- 230000008018 melting Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000010369 molecular cloning Methods 0.000 description 2
- 238000005457 optimization Methods 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- -1 rRNA Proteins 0.000 description 2
- 230000001105 regulatory effect Effects 0.000 description 2
- 238000012216 screening Methods 0.000 description 2
- 230000035945 sensitivity Effects 0.000 description 2
- BAZVSMNPJJMILC-UHFFFAOYSA-N triadimenol Chemical compound C1=NC=NN1C(C(O)C(C)(C)C)OC1=CC=C(Cl)C=C1 BAZVSMNPJJMILC-UHFFFAOYSA-N 0.000 description 2
- 239000001226 triphosphate Substances 0.000 description 2
- 235000011178 triphosphate Nutrition 0.000 description 2
- UHPMCKVQTMMPCG-UHFFFAOYSA-N 5,8-dihydroxy-2-methoxy-6-methyl-7-(2-oxopropyl)naphthalene-1,4-dione Chemical compound CC1=C(CC(C)=O)C(O)=C2C(=O)C(OC)=CC(=O)C2=C1O UHPMCKVQTMMPCG-UHFFFAOYSA-N 0.000 description 1
- 108020005544 Antisense RNA Proteins 0.000 description 1
- 235000007319 Avena orientalis Nutrition 0.000 description 1
- 244000075850 Avena orientalis Species 0.000 description 1
- 241001465180 Botrytis Species 0.000 description 1
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 1
- 241001157813 Cercospora Species 0.000 description 1
- 241000218631 Coniferophyta Species 0.000 description 1
- 108091035707 Consensus sequence Proteins 0.000 description 1
- 241000371644 Curvularia ravenelii Species 0.000 description 1
- 108010052832 Cytochromes Proteins 0.000 description 1
- 102000018832 Cytochromes Human genes 0.000 description 1
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
- 241000221785 Erysiphales Species 0.000 description 1
- 241000223218 Fusarium Species 0.000 description 1
- 241000593551 Gremmeniella abietina Species 0.000 description 1
- 108091092195 Intron Proteins 0.000 description 1
- 241000222058 Kabatiella Species 0.000 description 1
- 240000008790 Musa x paradisiaca Species 0.000 description 1
- 235000018290 Musa x paradisiaca Nutrition 0.000 description 1
- 241000131448 Mycosphaerella Species 0.000 description 1
- 238000010222 PCR analysis Methods 0.000 description 1
- 108010010677 Phosphodiesterase I Proteins 0.000 description 1
- 108020005120 Plant DNA Proteins 0.000 description 1
- 241000209504 Poaceae Species 0.000 description 1
- 241000184297 Pseudocercospora musae Species 0.000 description 1
- 241000221300 Puccinia Species 0.000 description 1
- 241000228453 Pyrenophora Species 0.000 description 1
- 108020004511 Recombinant DNA Proteins 0.000 description 1
- 241000209056 Secale Species 0.000 description 1
- 102000039471 Small Nuclear RNA Human genes 0.000 description 1
- 108020004688 Small Nuclear RNA Proteins 0.000 description 1
- 241000592344 Spermatophyta Species 0.000 description 1
- 239000007983 Tris buffer Substances 0.000 description 1
- 244000098338 Triticum aestivum Species 0.000 description 1
- 241001123669 Verticillium albo-atrum Species 0.000 description 1
- 241001123668 Verticillium dahliae Species 0.000 description 1
- 238000011948 assay development Methods 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 235000015278 beef Nutrition 0.000 description 1
- RIOXQFHNBCKOKP-UHFFFAOYSA-N benomyl Chemical compound C1=CC=C2N(C(=O)NCCCC)C(NC(=O)OC)=NC2=C1 RIOXQFHNBCKOKP-UHFFFAOYSA-N 0.000 description 1
- MITFXPHMIHQXPI-UHFFFAOYSA-N benzoxaprofen Natural products N=1C2=CC(C(C(O)=O)C)=CC=C2OC=1C1=CC=C(Cl)C=C1 MITFXPHMIHQXPI-UHFFFAOYSA-N 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 229940098773 bovine serum albumin Drugs 0.000 description 1
- 239000012677 causal agent Substances 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000010367 cloning Methods 0.000 description 1
- 239000003184 complementary RNA Substances 0.000 description 1
- 244000038559 crop plants Species 0.000 description 1
- 238000005520 cutting process Methods 0.000 description 1
- SUYVUBYJARFZHO-RRKCRQDMSA-N dATP Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@H]1C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 SUYVUBYJARFZHO-RRKCRQDMSA-N 0.000 description 1
- SUYVUBYJARFZHO-UHFFFAOYSA-N dATP Natural products C1=NC=2C(N)=NC=NC=2N1C1CC(O)C(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 SUYVUBYJARFZHO-UHFFFAOYSA-N 0.000 description 1
- RGWHQCVHVJXOKC-SHYZEUOFSA-J dCTP(4-) Chemical compound O=C1N=C(N)C=CN1[C@@H]1O[C@H](COP([O-])(=O)OP([O-])(=O)OP([O-])([O-])=O)[C@@H](O)C1 RGWHQCVHVJXOKC-SHYZEUOFSA-J 0.000 description 1
- HAAZLUGHYHWQIW-KVQBGUIXSA-N dGTP Chemical compound C1=NC=2C(=O)NC(N)=NC=2N1[C@H]1C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 HAAZLUGHYHWQIW-KVQBGUIXSA-N 0.000 description 1
- NHVNXKFIZYSCEB-XLPZGREQSA-N dTTP Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)[C@@H](O)C1 NHVNXKFIZYSCEB-XLPZGREQSA-N 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 230000001066 destructive effect Effects 0.000 description 1
- 238000012631 diagnostic technique Methods 0.000 description 1
- LEOJDCQCOZOLTQ-UHFFFAOYSA-N dibutylcarbamothioyl n,n-dibutylcarbamodithioate Chemical compound CCCCN(CCCC)C(=S)SC(=S)N(CCCC)CCCC LEOJDCQCOZOLTQ-UHFFFAOYSA-N 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 210000002257 embryonic structure Anatomy 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000011536 extraction buffer Substances 0.000 description 1
- 239000007850 fluorescent dye Substances 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 1
- 230000004927 fusion Effects 0.000 description 1
- 239000003630 growth substance Substances 0.000 description 1
- 238000009396 hybridization Methods 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000002803 maceration Methods 0.000 description 1
- 229910001629 magnesium chloride Inorganic materials 0.000 description 1
- 210000001161 mammalian embryo Anatomy 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 108020004999 messenger RNA Proteins 0.000 description 1
- 230000002438 mitochondrial effect Effects 0.000 description 1
- 239000003068 molecular probe Substances 0.000 description 1
- 238000002887 multiple sequence alignment Methods 0.000 description 1
- 230000002956 necrotizing effect Effects 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 239000002777 nucleoside Substances 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 230000003071 parasitic effect Effects 0.000 description 1
- 230000008659 phytopathology Effects 0.000 description 1
- 238000003976 plant breeding Methods 0.000 description 1
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 description 1
- 229920000053 polysorbate 80 Polymers 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 238000012207 quantitative assay Methods 0.000 description 1
- 239000011541 reaction mixture Substances 0.000 description 1
- 230000029610 recognition of host Effects 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 230000002103 transcriptional effect Effects 0.000 description 1
- 150000003852 triazoles Chemical class 0.000 description 1
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 1
- 239000011800 void material Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/16—Primer sets for multiplex assays
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/166—Oligonucleotides used as internal standards, controls or normalisation probes
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Immunology (AREA)
- Mycology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Botany (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
L'invention concerne des amorceurs et des sondes à utiliser dans des dosages TaqMan?TM¿ quantitatifs de PCR permettant de détecter Tapesia yallundae (syn. Pseudocercosporella herpotrichoides type W) et Tapesia acuformis (syn. Pseudocercosporella herpotrichoides type R). L'invention traite également d'amorceurs et de sondes à utiliser dans des dosages de contrôle TaqMan?TM¿ quantitatifs pour la détection de l'ADN du blé.
Applications Claiming Priority (5)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US37174999A | 1999-08-10 | 1999-08-10 | |
US09/371,749 | 1999-08-10 | ||
US16832699P | 1999-12-01 | 1999-12-01 | |
US60/168,326 | 1999-12-01 | ||
PCT/EP2000/007708 WO2001011075A2 (fr) | 1999-08-10 | 2000-08-08 | Detection et quantification basees sur l'amplification en chaine par polymerase de tapesia yallundae et tapesia acuformis |
Publications (1)
Publication Number | Publication Date |
---|---|
CA2381204A1 true CA2381204A1 (fr) | 2001-02-15 |
Family
ID=26864003
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA002381204A Abandoned CA2381204A1 (fr) | 1999-08-10 | 2000-08-08 | Detection et quantification basees sur l'amplification en chaine par polymerase de tapesia yallundae et tapesia acuformis |
Country Status (7)
Country | Link |
---|---|
EP (1) | EP1203097A2 (fr) |
JP (1) | JP2003506066A (fr) |
CN (1) | CN1249253C (fr) |
AU (1) | AU6440500A (fr) |
CA (1) | CA2381204A1 (fr) |
HK (1) | HK1046298A1 (fr) |
WO (1) | WO2001011075A2 (fr) |
Families Citing this family (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
AU2003230759A1 (en) * | 2002-04-03 | 2003-10-20 | Syngenta Participations Ag | Detection of wheat and barley fungal pathogens which are resistant to certain fungicides using the polymerase chain reaction |
DE102007010311A1 (de) * | 2007-02-23 | 2008-08-28 | Thines, Marco, Dr. | Organismusspezifisches hybridisierbares Nucleinsäuremolekül |
CN104846064B (zh) * | 2014-03-10 | 2017-06-30 | 浙江省农业科学院 | 一种早期检测杨梅是否发生凋萎病的方法 |
CN104232748B (zh) * | 2014-03-10 | 2016-05-18 | 浙江省农业科学院 | 一种杨梅苗木是否携带凋萎病菌的快速分子检测方法 |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5585238A (en) * | 1994-04-25 | 1996-12-17 | Ciba-Geigy Corporation | Detection of fungal pathogens using the polymerase chain reaction |
US5814453A (en) * | 1996-10-15 | 1998-09-29 | Novartis Finance Corporation | Detection of fungal pathogens using the polymerase chain reaction |
-
2000
- 2000-08-08 JP JP2001515323A patent/JP2003506066A/ja active Pending
- 2000-08-08 CN CNB008114404A patent/CN1249253C/zh not_active Expired - Fee Related
- 2000-08-08 EP EP00951484A patent/EP1203097A2/fr not_active Withdrawn
- 2000-08-08 WO PCT/EP2000/007708 patent/WO2001011075A2/fr not_active Application Discontinuation
- 2000-08-08 AU AU64405/00A patent/AU6440500A/en not_active Abandoned
- 2000-08-08 CA CA002381204A patent/CA2381204A1/fr not_active Abandoned
-
2002
- 2002-10-16 HK HK02107510.4A patent/HK1046298A1/zh unknown
Also Published As
Publication number | Publication date |
---|---|
WO2001011075A3 (fr) | 2001-12-06 |
HK1046298A1 (zh) | 2003-01-03 |
AU6440500A (en) | 2001-03-05 |
JP2003506066A (ja) | 2003-02-18 |
CN1369017A (zh) | 2002-09-11 |
EP1203097A2 (fr) | 2002-05-08 |
WO2001011075A2 (fr) | 2001-02-15 |
CN1249253C (zh) | 2006-04-05 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
EP0955381B1 (fr) | Détection des pathogènes fongiques utilisant la réaction de polymérase en chaine | |
EP0859061A2 (fr) | Détection des pathogènes fongiques de mais utilisant la réaction de polymérase en chaíne | |
EP1002134B1 (fr) | Detection de pathogenes fongiques du ble a l'aide de l'amplification en chaine par polymerase | |
US6485907B1 (en) | PCR-based detection of Rhizoctonia cerealis | |
AU744803B2 (en) | Detection of wheat and barley fungal pathogens using the polymerase chain reaction | |
US20030113722A1 (en) | Detection of fusarium species infecting corn using the polymerase chain reaction | |
US5814453A (en) | Detection of fungal pathogens using the polymerase chain reaction | |
US6319673B1 (en) | PCR-based detection and quantification of Tapesia yallundae and Tapesia acuformis | |
AU765083B2 (en) | Detection of (monilinia) spp. using the polymerase chain reaction | |
CA2381204A1 (fr) | Detection et quantification basees sur l'amplification en chaine par polymerase de tapesia yallundae et tapesia acuformis | |
AU2004210551A1 (en) | PCR-based detection and quantification of Tapesia yallundae and Tapesia acuformis | |
EP1290229A2 (fr) | Detection de mycosphaerella en chaine de la polymerase | |
WO2001053521A2 (fr) | Oligonucleotides identifiant des champignons resistant aux fongicides | |
WO2003085378A2 (fr) | Detection de pathogenes fongiques de ble et d'orge resistant a certains fongicides au moyen de la reaction en chaine de la polymerase |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
EEER | Examination request | ||
FZDE | Dead |