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CA2329031A1 - High numerical aperture flow cytometer and method of using same - Google Patents

High numerical aperture flow cytometer and method of using same Download PDF

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Publication number
CA2329031A1
CA2329031A1 CA002329031A CA2329031A CA2329031A1 CA 2329031 A1 CA2329031 A1 CA 2329031A1 CA 002329031 A CA002329031 A CA 002329031A CA 2329031 A CA2329031 A CA 2329031A CA 2329031 A1 CA2329031 A1 CA 2329031A1
Authority
CA
Canada
Prior art keywords
laser input
flow cell
angle
oriented
right angle
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002329031A
Other languages
French (fr)
Other versions
CA2329031C (en
Inventor
Anthony A. Ferrante
W. Peter Hansen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Idexx Laboratories Inc
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2329031A1 publication Critical patent/CA2329031A1/en
Application granted granted Critical
Publication of CA2329031C publication Critical patent/CA2329031C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/01—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials specially adapted for biological cells, e.g. blood cells
    • G01N2015/016—White blood cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1434—Optical arrangements
    • G01N2015/1447—Spatial selection

Landscapes

  • Chemical & Material Sciences (AREA)
  • Dispersion Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The high numerical aperture flow cytometer of the present invention includes a flow cell and a laser input. The laser input emits a beam of light that is oriented substantially orthoganilly to the flow of blood cells through the flow cell such that laser light impinges upon the blood cells as they pass through the flow cell. A portion of the beam from the laser input that impinges upon the blood cells in the flow cell is scattered at a substantially right angle to the beam of laser input ("right angle scatter"). A second portion of the beam from the laser input that impinges upon the cells in the flow cell is scattered at a much lower angle than 90~. This scatter is termed "low angle forward scatter light" and has an angle of from about 2~ to about 5~ from the orientation of the original beam from laser input. A right angle scatter light detector is oriented to receive the previously mentioned right angle scatter light. A low angle forward scatter light detector is oriented to capture the previously mentioned low angled forward scatter light oriented at about 2~ to about 5~ beam from laser input.
CA002329031A 1999-02-19 2000-02-18 High numerical aperture flow cytometer and method of using same Expired - Fee Related CA2329031C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US12080499P 1999-02-19 1999-02-19
US60/120,804 1999-02-19
PCT/US2000/004069 WO2000049387A2 (en) 1999-02-19 2000-02-18 High numerical aperture flow cytometer and method of using same

Publications (2)

Publication Number Publication Date
CA2329031A1 true CA2329031A1 (en) 2000-08-24
CA2329031C CA2329031C (en) 2003-09-23

Family

ID=22392650

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002329031A Expired - Fee Related CA2329031C (en) 1999-02-19 2000-02-18 High numerical aperture flow cytometer and method of using same

Country Status (5)

Country Link
EP (1) EP1095256A2 (en)
JP (1) JP2002537557A (en)
AU (1) AU768616C (en)
CA (1) CA2329031C (en)
WO (1) WO2000049387A2 (en)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE69830598T2 (en) 1997-01-31 2006-05-18 The Horticulture And Food Research Institute Of New Zealand Limited OPTICAL DEVICE AND METHOD
US6149867A (en) 1997-12-31 2000-11-21 Xy, Inc. Sheath fluids and collection systems for sex-specific cytometer sorting of sperm
US6071689A (en) 1997-12-31 2000-06-06 Xy, Inc. System for improving yield of sexed embryos in mammals
US6618143B2 (en) 2000-02-18 2003-09-09 Idexx Laboratories, Inc. High numerical aperture flow cytometer and method of using same
US7094527B2 (en) 2000-11-29 2006-08-22 Xy, Inc. System for in-vitro fertilization with spermatozoa separated into X-chromosome and Y-chromosome bearing populations
US7169548B2 (en) 2002-09-13 2007-01-30 Xy, Inc. Sperm cell processing and preservation systems
DE602004024874D1 (en) 2003-03-28 2010-02-11 Inguran Llc EASTER-ASSORTED TIERSPERMIES

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4818103A (en) * 1981-05-15 1989-04-04 Ratcom Flow cytometry
US4606636A (en) * 1983-10-25 1986-08-19 Universite De Saint-Etienne Optical apparatus for identifying the individual multiparametric properties of particles or bodies in a continuous flow
NL8601000A (en) * 1986-04-21 1987-11-16 Jan Greve T H Twente Afdeling THE USE OF POLARIZED LIGHT IN FLOW CYTOMETRY.
US5057413A (en) * 1988-06-13 1991-10-15 Becton, Dickinson And Company Method for discriminating between intact and damaged cells in a sample
US4954715A (en) * 1989-06-26 1990-09-04 Zoeld Tibor Method and apparatus for an optimized multiparameter flow-through particle and cell analyzer
ES2311659T3 (en) * 1994-08-01 2009-02-16 Abbott Laboratories METHOD AND APPARATUS OF PREPARATION OF A FLUID.
US5631165A (en) * 1994-08-01 1997-05-20 Abbott Laboratories Method for performing automated hematology and cytometry analysis
JP3324050B2 (en) * 1994-10-31 2002-09-17 日本光電工業株式会社 Leukocyte classification reagent and leukocyte classification method
US5650847A (en) * 1995-06-14 1997-07-22 Erkki Soini Method and device for determination of parameters of individual microparticles
JP3504029B2 (en) * 1995-07-04 2004-03-08 シスメックス株式会社 Particle analyzer
DE59710824D1 (en) * 1996-05-03 2003-11-13 Ciba Sc Holding Ag High capacity optical storage media containing xanthene dyes
DE19700648A1 (en) * 1997-01-10 1998-07-23 Basf Ag Method and device for determining the size distribution of different types of particles in a sample
JP3642658B2 (en) * 1997-06-30 2005-04-27 シスメックス株式会社 Urine component analyzer and analysis method

Also Published As

Publication number Publication date
WO2000049387A2 (en) 2000-08-24
CA2329031C (en) 2003-09-23
WO2000049387A3 (en) 2001-02-15
WO2000049387A9 (en) 2001-09-27
AU3597600A (en) 2000-09-04
AU768616C (en) 2004-12-16
EP1095256A2 (en) 2001-05-02
JP2002537557A (en) 2002-11-05
AU768616B2 (en) 2003-12-18

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Effective date: 20180219