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CA2301818A1 - Bifunctional compounds useful in catalyst reporter deposition - Google Patents

Bifunctional compounds useful in catalyst reporter deposition Download PDF

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Publication number
CA2301818A1
CA2301818A1 CA002301818A CA2301818A CA2301818A1 CA 2301818 A1 CA2301818 A1 CA 2301818A1 CA 002301818 A CA002301818 A CA 002301818A CA 2301818 A CA2301818 A CA 2301818A CA 2301818 A1 CA2301818 A1 CA 2301818A1
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CA
Canada
Prior art keywords
reporter
analyte
deposition
conjugate
activation system
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002301818A
Other languages
French (fr)
Other versions
CA2301818C (en
Inventor
Richard Calvin Ebersole
John Richard Moran
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Revvity Health Sciences Inc
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Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Priority claimed from CA 2013214 external-priority patent/CA2013214C/en
Publication of CA2301818A1 publication Critical patent/CA2301818A1/en
Application granted granted Critical
Publication of CA2301818C publication Critical patent/CA2301818C/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The present invention concerns a method to catalyze reporter deposition to improve detection or quantitation of an analyte in a sample by amplifying the detector signal which comprises immobilizing an analyte dependent enzyme activation system which catalyzes deposition of reporter by activating a conjugate consisting of a detectably labeled substrate specific for the enzyme system, said conjugate reacts with the analyte dependent enzyme activation system to form an activated conjugate which deposits substantially wherever receptor for the activated conjugate is immobilized, said receptor not being reactive with the analyte dependent enzyme activation system. In another embodiment the invention concerns an assay for detecting or quantitating the presence or absence of an analyte in a sample using catalyzed reporter deposition to amplify the reporter signal. Also described are novel compounds which can be used as reagents to prepare HABA-type conjugates.
CA002301818A 1989-03-29 1990-03-28 Bifunctional compounds useful in catalyst reporter deposition Expired - Lifetime CA2301818C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US33035789A 1989-03-29 1989-03-29
US07/330,357 1989-03-29
CA 2013214 CA2013214C (en) 1989-03-29 1990-03-28 Catalyzed reporter deposition

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
CA 2013214 Division CA2013214C (en) 1989-03-29 1990-03-28 Catalyzed reporter deposition

Publications (2)

Publication Number Publication Date
CA2301818A1 true CA2301818A1 (en) 1990-09-29
CA2301818C CA2301818C (en) 2004-10-26

Family

ID=25674033

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002301818A Expired - Lifetime CA2301818C (en) 1989-03-29 1990-03-28 Bifunctional compounds useful in catalyst reporter deposition

Country Status (1)

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CA (1) CA2301818C (en)

Also Published As

Publication number Publication date
CA2301818C (en) 2004-10-26

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