[go: up one dir, main page]

CA2293810C - Desaturases d'acide gras et leurs sequences mutantes - Google Patents

Desaturases d'acide gras et leurs sequences mutantes Download PDF

Info

Publication number
CA2293810C
CA2293810C CA002293810A CA2293810A CA2293810C CA 2293810 C CA2293810 C CA 2293810C CA 002293810 A CA002293810 A CA 002293810A CA 2293810 A CA2293810 A CA 2293810A CA 2293810 C CA2293810 C CA 2293810C
Authority
CA
Canada
Prior art keywords
fatty acid
delta
plants
gene
mutation
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
CA002293810A
Other languages
English (en)
Other versions
CA2293810A1 (fr
Inventor
Lorin R. Debonte
Zhegong Fan
Basil S. Shorrosh
Guo-Hua Miao
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Cargill Inc
Original Assignee
Cargill Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cargill Inc filed Critical Cargill Inc
Priority to CA002575962A priority Critical patent/CA2575962A1/fr
Publication of CA2293810A1 publication Critical patent/CA2293810A1/fr
Application granted granted Critical
Publication of CA2293810C publication Critical patent/CA2293810C/fr
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0083—Miscellaneous (1.14.99)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8247—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified lipid metabolism, e.g. seed oil composition

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Nutrition Science (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Medicinal Chemistry (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

La présente invention concerne des graines, des plantes et des huiles présentant une teneur élevée en acide oléique; une faible teneur en acide linoléique; une faible teneur en acide linoléique plus un acide linolénique; et des propriétés fonctionnelles ou alimentaires avantageuses. L'invention concerne des plantes présentant une mutation dans un gène à désaturases d'acide gras delta-12 ou delta-15. Les plantes sont, de préférence, du colza et du tournesol. Les plantes porteuses de tels gènes mutants présentent, dans leurs graines, une composition d'acide gras modifié. Selon un mode de réalisation, la plante présente une mutation dans une région comportant le motif conservé His-Xaa-Xaa-Xaa-His, rencontré dans les désaturases d'acide gras delta-12 et delta-15. Un motif préféré comporte la séquence His-Glu-Cys-Gly-His. Une mutation préférée de ce motif comporte la séquence d'acides aminés His-Lys-Cys-Gly-His. L'invention concerne, en outre, des fragments d'acide nucléique renfermant une séquence mutante de gènes à désaturases d'acide gras delta-12 ou delta-15.
CA002293810A 1997-06-12 1998-06-11 Desaturases d'acide gras et leurs sequences mutantes Expired - Lifetime CA2293810C (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CA002575962A CA2575962A1 (fr) 1997-06-12 1998-06-11 Desaturases d'acides gras et sequences mutantes de ces enzymes

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US87410997A 1997-06-12 1997-06-12
US08/874,109 1997-06-12
PCT/US1998/012332 WO1998056239A1 (fr) 1997-06-12 1998-06-11 Desaturases d'acide gras et leurs sequences mutantes

Related Child Applications (1)

Application Number Title Priority Date Filing Date
CA002575962A Division CA2575962A1 (fr) 1997-06-12 1998-06-11 Desaturases d'acides gras et sequences mutantes de ces enzymes

Publications (2)

Publication Number Publication Date
CA2293810A1 CA2293810A1 (fr) 1998-12-17
CA2293810C true CA2293810C (fr) 2010-03-09

Family

ID=25362994

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002293810A Expired - Lifetime CA2293810C (fr) 1997-06-12 1998-06-11 Desaturases d'acide gras et leurs sequences mutantes

Country Status (5)

Country Link
EP (1) EP0987936A4 (fr)
JP (1) JP4209949B2 (fr)
AU (1) AU750363B2 (fr)
CA (1) CA2293810C (fr)
WO (1) WO1998056239A1 (fr)

Families Citing this family (31)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6483008B1 (en) * 1990-08-15 2002-11-19 Calgene Llc Methods for producing plants with elevated oleic acid content
JP2003512810A (ja) * 1998-08-04 2003-04-08 カーギル インコーポレイテッド 植物の脂肪酸デサチュラーゼプロモーター
US6713117B1 (en) 1998-10-02 2004-03-30 Dharma Kodali Vegetable oil having elevated stearic acid content
CA2284246A1 (fr) 1999-10-01 2001-04-01 Agriculture And Agrifood Canada Of Agriculture And Agri-Food Desaturases d'acides gras vegetaux et leurs alleles
CA2408357A1 (fr) * 2000-05-09 2001-11-15 Bioriginal Food & Science Corp. Production d'acides linoleiques et d'acides linoleiques conjugues dans des plantes
US7566813B2 (en) 2002-03-21 2009-07-28 Monsanto Technology, L.L.C. Nucleic acid constructs and methods for producing altered seed oil compositions
US8304610B2 (en) 2002-05-15 2012-11-06 Viterra, Inc. Brassica juncea lines with high oleic acid profile in seed oil
CA2530566A1 (fr) * 2003-05-30 2005-01-06 Cargill, Incorporated Methodes de production de plantes qui presentent une meilleure resistance aux maladies
CN103451246B (zh) 2004-04-22 2018-02-16 联邦科学技术研究组织 用重组细胞合成长链多不饱和脂肪酸
CA2884237C (fr) 2004-04-22 2020-09-08 Commonwealth Scientific And Industrial Research Organisation Synthese d'acides gras polyinsatures a chaine longue par des cellules de recombinaison
AU2004203364B2 (en) * 2004-07-15 2011-04-07 Nutrien Ag Solutions (Canada) Inc. High oleic acid brassica juncea
EP1806398A1 (fr) * 2006-01-04 2007-07-11 Monsanto S.A.S. Mutants de FAD-2 et plantes à teneur élevée en acide oléique
CN101421406B (zh) 2006-02-13 2016-08-31 孟山都技术有限公司 用于产生改变的种子油组成的核酸构建体和方法
EP1837397A1 (fr) * 2006-03-21 2007-09-26 Monsanto S.A.S. Mutants de FAD-2 et plantes à teneur élevée en acide oléique
EP2059588A4 (fr) 2006-08-29 2010-07-28 Commw Scient Ind Res Org Synthèse d'acides gras
MX356687B (es) 2008-09-29 2018-06-07 Monsanto Technology Llc Evento transgénico de frijol de soya mon87705 y métodos para detección del mismo.
BRPI0921467B1 (pt) 2008-11-18 2021-12-14 Commonwealth Scientific And Industrial Research Organisation Processo para a produção de óleo compreendendo ácido eicosapentaenóico (epa), ácido docosapentaenóico (dpa) e ácido docosahexaenóico (dha)
US9035129B2 (en) * 2009-07-08 2015-05-19 The Curators Of The University Of Missouri Method to develop high oleic acid soybeans using conventional soybean breeding techniques
WO2011075716A1 (fr) 2009-12-18 2011-06-23 Cargill, Incorporated Plantes du genre brassica donnant des huiles à faible teneur totale en acides gras saturés
US9695434B2 (en) 2010-05-25 2017-07-04 Cargill, Incorporated Brassica plants yielding oils with a low alpha linolenic acid content
EP2576765A4 (fr) 2010-05-25 2013-12-18 Cargill Inc Plantes du genre brassica donnant des huiles à faible teneur en acide alpha-linolénique
GB201103569D0 (en) * 2011-03-02 2011-04-13 Plant Bioscience Ltd Mutants
NZ631702A (en) 2012-06-15 2017-01-27 Grains Res & Dev Corp Production of long chain polyunsaturated fatty acids in plant cells
UA118090C2 (uk) * 2012-09-07 2018-11-26 ДАУ АГРОСАЙЄНСІЗ ЕлЕлСі Спосіб інтегрування послідовності нуклеїнової кислоти, що представляє інтерес, у ген fad2 у клітині сої та специфічний для локусу fad2 білок, що зв'язується, здатний індукувати спрямований розрив
KR102535223B1 (ko) 2013-12-18 2023-05-30 커먼웰쓰 사이언티픽 앤 인더스트리알 리서치 오거니제이션 장쇄 다중불포화 지방산을 포함하는 지질
WO2015196250A1 (fr) 2014-06-27 2015-12-30 Commonwealth Scientific And Industrial Research Organisation Lipide comprenant de l'acide docosapentaénoïque
AU2016256239B2 (en) 2015-04-28 2021-08-19 BASF Agricultural Solutions Seed US LLC Brassica plants with modified seed oil composition
CA3047768A1 (fr) 2019-06-21 2020-12-21 BASF Agricultural Solutions Seed US LLC Variete de canola hybride 7cn0425
US11997965B2 (en) 2020-04-24 2024-06-04 BASF Agricultural Solutions Seed US LLC Canola hybrid variety 8CN0001
US11672218B2 (en) 2020-04-24 2023-06-13 BASF Agricultural Solutions Seed US LLC Canola hybrid variety 7CN0065
US11997966B2 (en) 2020-04-24 2024-06-04 BASF Agricultural Solutions Seed US LLC Canola hybrid variety 7CN0020

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU675923B2 (en) * 1991-12-04 1997-02-27 E.I. Du Pont De Nemours And Company Fatty acid desaturase genes from plants
AU5407594A (en) * 1992-11-17 1994-06-08 E.I. Du Pont De Nemours And Company Genes for microsomal delta-12 fatty acid desaturases and related enzymes from plants
GB9324823D0 (en) * 1993-12-02 1994-01-19 Davy Mckee London Process
EP0880312B1 (fr) * 1995-12-14 2006-03-08 Cargill Incorporated Vegetaux comportant des sequences mutantes conferant des profils d'acides gras modifies
CA2547678A1 (fr) * 1996-07-03 1998-01-04 Cargill, Incorporated Huile de colza possedant une teneur plus elevee en acide oleique et en acide linolenique

Also Published As

Publication number Publication date
JP2002508659A (ja) 2002-03-19
EP0987936A4 (fr) 2001-03-14
CA2293810A1 (fr) 1998-12-17
AU8071598A (en) 1998-12-30
AU750363B2 (en) 2002-07-18
EP0987936A1 (fr) 2000-03-29
WO1998056239A1 (fr) 1998-12-17
JP4209949B2 (ja) 2009-01-14

Similar Documents

Publication Publication Date Title
CA2293810C (fr) Desaturases d'acide gras et leurs sequences mutantes
US6967243B2 (en) Fatty acid desaturases and mutant sequences thereof
CA2238964C (fr) Vegetaux comportant des sequences mutantes conferant des profils d'acides gras modifies
EP1837397A1 (fr) Mutants de FAD-2 et plantes à teneur élevée en acide oléique
US6649782B2 (en) Plants, seeds and oils having an elevated total monounsaturated fatty acid content
AU2008202988A1 (en) Plant fad2 coding sequence balancing for fatty acid profiling in edible oils
US7541519B2 (en) Fatty acid desaturases and mutant sequences thereof
US7262343B1 (en) Plants having mutant delta-12 desaturase sequences that confer altered fatty acid profiles
AU2005244560B2 (en) Fatty acid desaturases and mutant sequences thereof
AU2002301539B2 (en) Fatty acid desaturases and mutant sequences thereof
MXPA99011601A (en) Fatty acid desaturases and mutant sequences thereof
AU2303001A (en) Plants having mutant sequences that confer altered fatty acid profiles
MXPA01001224A (en) Plants, seeds and oils having an elevated total monounsaturated fatty acid content

Legal Events

Date Code Title Description
EEER Examination request
MKEX Expiry

Effective date: 20180611