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CA2270289A1 - Proteins with enhanced levels of essential amino acids - Google Patents

Proteins with enhanced levels of essential amino acids

Info

Publication number
CA2270289A1
CA2270289A1 CA002270289A CA2270289A CA2270289A1 CA 2270289 A1 CA2270289 A1 CA 2270289A1 CA 002270289 A CA002270289 A CA 002270289A CA 2270289 A CA2270289 A CA 2270289A CA 2270289 A1 CA2270289 A1 CA 2270289A1
Authority
CA
Canada
Prior art keywords
amino acids
essential amino
proteins
enhanced levels
polypeptides
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002270289A
Other languages
French (fr)
Other versions
CA2270289C (en
Inventor
Aragula Gururaj Rao
Keith R. Roesler
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Pioneer Hi Bred International Inc
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2270289A1 publication Critical patent/CA2270289A1/en
Application granted granted Critical
Publication of CA2270289C publication Critical patent/CA2270289C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8251—Amino acid content, e.g. synthetic storage proteins, altering amino acid biosynthesis
    • C12N15/8253—Methionine or cysteine
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/142—Amino acids; Derivatives thereof
    • A23K20/147—Polymeric derivatives, e.g. peptides or proteins
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/17—Amino acids, peptides or proteins
    • A23L33/185—Vegetable proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/81—Protease inhibitors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/81—Protease inhibitors
    • C07K14/8107—Endopeptidase (E.C. 3.4.21-99) inhibitors
    • C07K14/811—Serine protease (E.C. 3.4.21) inhibitors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8251—Amino acid content, e.g. synthetic storage proteins, altering amino acid biosynthesis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8251—Amino acid content, e.g. synthetic storage proteins, altering amino acid biosynthesis
    • C12N15/8254—Tryptophan or lysine

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Polymers & Plastics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Nutrition Science (AREA)
  • Microbiology (AREA)
  • Food Science & Technology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Animal Husbandry (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)

Abstract

The present invention provides for polypeptides comprising protease inhibitors with increased amounts of essential amino acids and nucleotides encoding for these peptides. Also provided are transformed plants and seeds with enhanced nutritional value due to the expression of modified polypeptides.
CA002270289A 1996-11-01 1997-10-31 Proteins with enhanced levels of essential amino acids Expired - Fee Related CA2270289C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US74068296A 1996-11-01 1996-11-01
US08/740,682 1996-11-01
PCT/US1997/020441 WO1998020133A2 (en) 1996-11-01 1997-10-31 Proteins with enhanced levels of essential amino acids

Publications (2)

Publication Number Publication Date
CA2270289A1 true CA2270289A1 (en) 1998-05-14
CA2270289C CA2270289C (en) 2005-09-27

Family

ID=24977593

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002270289A Expired - Fee Related CA2270289C (en) 1996-11-01 1997-10-31 Proteins with enhanced levels of essential amino acids

Country Status (5)

Country Link
EP (1) EP0946729A2 (en)
AU (1) AU728086B2 (en)
CA (1) CA2270289C (en)
HU (1) HUP0000810A3 (en)
WO (1) WO1998020133A2 (en)

Families Citing this family (90)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6171640B1 (en) * 1997-04-04 2001-01-09 Monsanto Company High beta-conglycinin products and their use
FR2803592A1 (en) 2000-01-06 2001-07-13 Aventis Cropscience Sa NOVEL DERIVATIVES OF 3-HYDROXYPICOLINIC ACID, PROCESS FOR THEIR PREPARATION AND FUNGICIDAL COMPOSITIONS CONTAINING SAME
FR2815969B1 (en) 2000-10-30 2004-12-10 Aventis Cropscience Sa TOLERANT PLANTS WITH HERBICIDES BY METABOLIC BYPASS
US20020188965A1 (en) 2001-04-20 2002-12-12 Zou-Yu Zhao Methods of transforming plants
CN1671849A (en) * 2002-07-29 2005-09-21 拉瓦勒大学 Methods of Enhancing the Nutritional Value of Plant Extracts
EP2261362A3 (en) 2005-05-25 2011-04-20 Pioneer Hi-Bred International Inc. Methods for improving crop plant architecture and yield
EP1902137A4 (en) * 2005-07-08 2009-05-06 Renessen Llc High tryptophan soybean meal
US7589257B2 (en) 2006-02-09 2009-09-15 Pioneer Hi-Bred International Inc. Genes for enhancing nitrogen utilization efficiency in crop plants
MX2008013354A (en) 2006-04-19 2008-11-10 Pioneer Hi Bred Int Isolated polynucleotide molecules corresponding to mutant and wild-type alleles of the maize d9 gene and methods of use.
US7964774B2 (en) 2008-05-14 2011-06-21 Monsanto Technology Llc Plants and seeds of spring canola variety SCV384196
EP2821490A3 (en) 2008-10-30 2015-04-22 Pioneer Hi-Bred International Inc. Manipulation of glutamine synthetases (GS) to improve nitrogen use efficiency and grain yield in higher plants
US8987553B2 (en) 2009-04-14 2015-03-24 Pioneer Hi Bred International Inc Modulation of ACC synthase improves plant yield under low nitrogen conditions
WO2010147825A1 (en) 2009-06-09 2010-12-23 Pioneer Hi-Bred International, Inc. Early endosperm promoter and methods of use
CA2768571A1 (en) 2009-07-24 2011-01-27 Pioneer Hi-Bred International, Inc. The use of dimerization domain component stacks to modulate plant architecture
BR112012003761A2 (en) 2009-08-20 2015-09-08 Pionner Hi Bred International Inc nitrate transporter polynucleotide, vector, expression cassette, host cell, transgenic plant, seed, method for increasing productivity in a plant, nt protein level modulation method in a plant cell and in a plant, nitrate uptake in plants, decoupling method in a plant nitrate that signals nitrate uptake, recombinant or isolated nitrate reductase (nr) polynucleotide.
CA2770854A1 (en) 2009-08-20 2011-02-24 Pioneer Hi-Bred International, Inc. Functional expression of shuffled yeast nitrate transporter (ynt1) in maize to improve nitrate uptake under low nitrate environment
WO2011056544A1 (en) 2009-10-26 2011-05-12 Pioneer Hi-Bred International, Inc. Somatic ovule specific promoter and methods of use
MX2012007855A (en) 2010-01-06 2013-06-05 Du Pont Identification of diurnal rhythms in photosynthetic and non-photosynthetic tissues from zea mays and use in improving crop plants.
US20120311743A1 (en) 2010-02-02 2012-12-06 Bayer Cropscience Ag Soybean transformation using hppd inhibitors as selection agents
AU2011328963B2 (en) 2010-11-17 2016-12-08 Pioneer Hi-Bred International, Inc. Prediction of phenotypes and traits based on the metabolome
WO2012129373A2 (en) 2011-03-23 2012-09-27 Pioneer Hi-Bred International, Inc. Methods for producing a complex transgenic trait locus
CN104080914A (en) 2011-06-21 2014-10-01 先锋国际良种公司 Methods and compositions for producing male sterile plants
EP2739739A1 (en) 2011-08-03 2014-06-11 E. I. Du Pont de Nemours and Company Methods and compositions for targeted integration in a plant
US20140298544A1 (en) 2011-10-28 2014-10-02 Pioneer Hi Bred International Inc Engineered PEP carboxylase variants for improved plant productivity
WO2013066805A1 (en) 2011-10-31 2013-05-10 Pioneer Hi-Bred International, Inc. Improving plant drought tolerance, nitrogen use efficiency and yield
WO2013096818A1 (en) 2011-12-21 2013-06-27 The Curators Of The University Of Missouri Soybean variety s05-11268
WO2013096810A1 (en) 2011-12-21 2013-06-27 The Curators Of The University Of Missouri Soybean variety s05-11482
WO2013103371A1 (en) 2012-01-06 2013-07-11 Pioneer Hi-Bred International, Inc. Ovule specific promoter and methods of use
US9006515B2 (en) 2012-01-06 2015-04-14 Pioneer Hi Bred International Inc Pollen preferred promoters and methods of use
AR089793A1 (en) 2012-01-27 2014-09-17 Du Pont METHODS AND COMPOSITIONS TO GENERATE COMPOSITE TRANSGENIC RISK LOCUS
WO2013138309A1 (en) 2012-03-13 2013-09-19 Pioneer Hi-Bred International, Inc. Genetic reduction of male fertility in plants
EA201491670A1 (en) 2012-03-13 2015-07-30 Пайонир Хай-Бред Интернэшнл, Инк. GENETIC REDUCTION OF MALE REPRODUCTIVE FUNCTION IN PLANTS
US8859857B2 (en) 2012-04-26 2014-10-14 Monsanto Technology Llc Plants and seeds of spring canola variety SCV259778
US8835720B2 (en) 2012-04-26 2014-09-16 Monsanto Technology Llc Plants and seeds of spring canola variety SCV967592
US8878009B2 (en) 2012-04-26 2014-11-04 Monsanto Technology, LLP Plants and seeds of spring canola variety SCV318181
US20140109259A1 (en) 2012-10-11 2014-04-17 Pioneer Hi Bred International Inc Guard Cell Promoters and Uses Thereof
BR112015008504A2 (en) 2012-10-15 2017-08-22 Pioneer Hi Bred Int METHOD FOR ENHANCED PESTICIDAL ACTIVITY, POLYPEPTIDE, NUCLEIC ACID MOLECULE, EXPRESSION CASSETTE, VECTOR, METHOD FOR OBTAINING A PLANT, METHOD FOR OBTAINING A PLANT CELL, PESTICIDE COMPOSITION, METHOD FOR PROTECTING A PLANT, METHOD FOR PROTECTING A PLANT PLANT, METHOD TO INCREASE THE RESISTANCE OF A PLANT
CA2889557A1 (en) 2012-11-20 2014-05-30 Pioneer Hi-Bred International., Inc. Engineering plants for efficient uptake and utilization of urea to improve crop production
WO2014164828A2 (en) 2013-03-11 2014-10-09 Pioneer Hi-Bred International, Inc. Methods and compositions employing a sulfonylurea-dependent stabilization domain
WO2014164014A1 (en) 2013-03-11 2014-10-09 Pioneer Hi-Bred International, Inc. Genes for improving nutrient uptake and abiotic stress tolerance in plants
WO2014164775A1 (en) 2013-03-11 2014-10-09 Pioneer Hi-Bred International, Inc. Methods and compositions to improve the spread of chemical signals in plants
WO2014164116A1 (en) 2013-03-13 2014-10-09 Pioneer Hi-Bred International, Inc. Functional expression of bacterial major facilitator superfamily (sfm) gene in maize to improve agronomic traits and grain yield
AU2014241045B2 (en) 2013-03-13 2017-08-31 Pioneer Hi-Bred International, Inc. Glyphosate application for weed control in brassica
WO2014164074A1 (en) 2013-03-13 2014-10-09 Pioneer Hi-Bred International, Inc. Enhanced nitrate uptake and nitrate translocation by over-expressing maize functional low-affinity nitrate transporters in transgenic maize
BR112015023304A2 (en) 2013-03-14 2017-07-18 Pioneer Hi Bred Int method to improve abiotic stress tolerance of a plant, plant and seed
CA2902002C (en) 2013-03-14 2023-08-01 Pioneer Hi-Bred International, Inc. Compositions and methods to control insect pests
WO2014150914A2 (en) 2013-03-15 2014-09-25 Pioneer Hi-Bred International, Inc. Phi-4 polypeptides and methods for their use
US20140283216A1 (en) 2013-03-15 2014-09-18 Pioneer Hi Bred International Inc Compositions and methods of use of acc oxidase polynucleotides and polypeptides
EP3578667B1 (en) 2013-04-17 2023-10-04 Pioneer Hi-Bred International, Inc. Methods for characterizing dna sequence composition in a genome
EP3032942B1 (en) 2013-08-16 2020-03-11 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
BR112016003561B8 (en) 2013-08-22 2022-11-01 Du Pont METHOD FOR PRODUCING A GENETIC MODIFICATION, METHOD FOR INTRODUCING A POLYNUCLEOTIDE OF INTEREST INTO THE GENOME OF A PLANT, METHOD FOR EDITING A SECOND GENE INTO A PLANT GENOME AND METHOD FOR GENERATING A GLYPHOSATE-RESISTANT CORN PLANT
EP3692786B1 (en) 2013-09-13 2022-11-02 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
EP3102592B1 (en) 2014-02-07 2020-05-20 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
AU2015288157A1 (en) 2014-07-11 2017-01-19 E. I. Du Pont De Nemours And Company Compositions and methods for producing plants resistant to glyphosate herbicide
US20170218384A1 (en) 2014-08-08 2017-08-03 Pioneer Hi-Bred International, Inc. Ubiquitin promoters and introns and methods of use
CN108064129A (en) 2014-09-12 2018-05-22 纳幕尔杜邦公司 The generation in the site-specific integration site of complex character locus and application method in corn and soybean
WO2016044092A1 (en) 2014-09-17 2016-03-24 Pioneer Hi Bred International Inc Compositions and methods to control insect pests
CN113372421B (en) 2014-10-16 2024-08-06 先锋国际良种公司 Insecticidal proteins and methods of use thereof
WO2016099916A1 (en) 2014-12-19 2016-06-23 E. I. Du Pont De Nemours And Company Polylactic acid compositions with accelerated degradation rate and increased heat stability
US11041158B2 (en) 2014-12-22 2021-06-22 AgBiome, Inc. Optimization methods for making a synthetic gene
EP3244727A4 (en) 2015-01-15 2018-10-31 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
BR112017017279A2 (en) 2015-03-19 2018-04-17 Pioneer Hi Bred Int methods for introducing a pollen inhibitor gene, for introducing two pollen inhibitor genes, for introducing two color marker and accelerated introgression trait and plant marker genes
CA2985198A1 (en) 2015-05-19 2016-11-24 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
BR112017027382A2 (en) 2015-06-16 2018-08-28 Pioneer Hi-Bred International, Inc. silencing element, dna construct, expression construct, expression cassette, host cell, composition, plant cell, plant or plant part, transgenic seed, method for controlling a plant insect pest, kit for controlling insect pests
ES2926808T3 (en) 2015-08-06 2022-10-28 Pioneer Hi Bred Int Plant-derived insecticidal proteins and methods of their use
EP3341483B1 (en) 2015-08-28 2019-12-18 Pioneer Hi-Bred International, Inc. Ochrobactrum-mediated transformation of plants
BR112018007796A2 (en) 2015-11-06 2018-10-30 Du Pont soybean plants, soybean plant parts or soybean seeds, method of selecting a soybean cell, methods of selecting a soybean cell and producing a locus and nucleic acid molecule
WO2017105987A1 (en) 2015-12-18 2017-06-22 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
EP4257694A3 (en) 2015-12-22 2023-12-06 Pioneer Hi-Bred International, Inc. Tissue-preferred promoters and methods of use
US9896696B2 (en) 2016-02-15 2018-02-20 Benson Hill Biosystems, Inc. Compositions and methods for modifying genomes
WO2017192560A1 (en) 2016-05-04 2017-11-09 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
US20190185867A1 (en) 2016-06-16 2019-06-20 Pioneer Hi-Bred International, Inc. Compositions and methods to control insect pests
MX2018015900A (en) 2016-06-24 2019-04-04 Pioneer Hi Bred Int Plant regulatory elements and methods of use thereof.
WO2018005411A1 (en) 2016-07-01 2018-01-04 Pioneer Hi-Bred International, Inc. Insecticidal proteins from plants and methods for their use
WO2018013333A1 (en) 2016-07-12 2018-01-18 Pioneer Hi-Bred International, Inc. Compositions and methods to control insect pests
US11140902B2 (en) 2016-09-27 2021-10-12 University Of Florida Research Foundation, Inc. Insect toxin delivery mediated by a densovirus coat protein
CA3038806A1 (en) 2016-11-01 2018-05-11 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
EP3619305A1 (en) 2017-05-03 2020-03-11 KWS SAAT SE & Co. KGaA Use of crispr-cas endonucleases for plant genome engineering
US10316324B2 (en) 2017-08-09 2019-06-11 Benson Hill Biosystems, Inc. Compositions and methods for modifying genomes
EP3688171A1 (en) 2017-09-25 2020-08-05 Pioneer Hi-Bred International, Inc. Tissue-preferred promoters and methods of use
CN111867377B (en) 2018-03-14 2023-05-23 先锋国际良种公司 Insecticidal proteins from plants and methods of use thereof
US11525144B2 (en) 2018-03-14 2022-12-13 Pioneer Hi-Bred International, Inc. Insecticidal proteins from plants and methods for their use
US11702668B2 (en) 2018-05-22 2023-07-18 Pioneer Hi-Bred International, Inc. Plant regulatory elements and methods of use thereof
CA3097915A1 (en) 2018-06-28 2020-01-02 Pioneer Hi-Bred International, Inc. Methods for selecting transformed plants
CA3111090A1 (en) 2018-10-31 2020-05-07 Pioneer Hi-Bred International, Inc. Compositions and methods for ochrobactrum-mediated plant transformation
MX2021014861A (en) 2019-06-25 2022-06-22 Inari Agriculture Tech Inc Improved homology dependent repair genome editing.
BR112022003996A2 (en) 2019-09-05 2022-05-31 Benson Hill Inc Compositions and methods for modifying genomes
US12168774B2 (en) 2020-07-14 2024-12-17 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
EP4453199A1 (en) 2021-12-21 2024-10-30 Benson Hill, Inc. Compositions and methods for modifying genomes
WO2023141464A1 (en) 2022-01-18 2023-07-27 AgBiome, Inc. Method for designing synthetic nucleotide sequences

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5096815A (en) * 1989-01-06 1992-03-17 Protein Engineering Corporation Generation and selection of novel dna-binding proteins and polypeptides
DK223790D0 (en) * 1990-09-18 1990-09-18 Novo Nordisk As PROTEASE-CONTAINING DETERGENT COMPOSITION
DK43192D0 (en) * 1992-03-31 1992-03-31 Novo Nordisk As detergent composition
WO1994010315A2 (en) * 1992-10-23 1994-05-11 Pioneer Hi-Bred International, Inc. Process for enhancing the content of a selected amino acid in a seed storage protein
DE69428290T2 (en) * 1993-01-13 2002-04-18 Pioneer Hi-Bred International, Inc. DERIVATIVES OF ALPHA-HORDOTHIONIN WITH HIGHER LEVELS OF LYSINE
DE69533616D1 (en) * 1994-04-04 2004-11-11 Pioneer Hi Bred Int THE ENDOGENE SEED PROTEIN CONTAINS REDUCTION IN PLANTS

Also Published As

Publication number Publication date
WO1998020133A2 (en) 1998-05-14
HUP0000810A2 (en) 2000-06-28
WO1998020133A3 (en) 1998-07-23
AU728086B2 (en) 2001-01-04
CA2270289C (en) 2005-09-27
AU5174998A (en) 1998-05-29
HUP0000810A3 (en) 2002-02-28
EP0946729A2 (en) 1999-10-06

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