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CA2264780A1 - Methods and materials for optimization of electronic hybridization reactions - Google Patents

Methods and materials for optimization of electronic hybridization reactions

Info

Publication number
CA2264780A1
CA2264780A1 CA002264780A CA2264780A CA2264780A1 CA 2264780 A1 CA2264780 A1 CA 2264780A1 CA 002264780 A CA002264780 A CA 002264780A CA 2264780 A CA2264780 A CA 2264780A CA 2264780 A1 CA2264780 A1 CA 2264780A1
Authority
CA
Canada
Prior art keywords
hybridization
hybridization reactions
cysteine
dna
optimization
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002264780A
Other languages
French (fr)
Other versions
CA2264780C (en
Inventor
Ronald George Sosnowski
William Frank Butler
Eugene Tu
Michael Irving Nerenberg
Michael James Heller
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Nanogen Inc
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2264780A1 publication Critical patent/CA2264780A1/en
Application granted granted Critical
Publication of CA2264780C publication Critical patent/CA2264780C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • H—ELECTRICITY
    • H10—SEMICONDUCTOR DEVICES; ELECTRIC SOLID-STATE DEVICES NOT OTHERWISE PROVIDED FOR
    • H10D—INORGANIC ELECTRIC SEMICONDUCTOR DEVICES
    • H10D64/00—Electrodes of devices having potential barriers
    • H10D64/01—Manufacture or treatment
    • H10D64/021—Manufacture or treatment using multiple gate spacer layers, e.g. bilayered sidewall spacers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6832—Enhancement of hybridisation reaction

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Electrochemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)

Abstract

The following inventions relate to discoveries concerning the various parameters, electrolytes (buffers), and other conditions which improve or optimize the speed of DNA transport, the efficiency of DNA
hybridization reactions, and the overall hybridization specificity in microelectronic chips and devices. In particular, this invention relates to the discovery that low conductance zwitterionic buffer solutions, especially those containing the amino acid Histidine prepared at concentrations of ~50 mM and at or near the pI (isoelectric point ~pH 7.47), provide optimal conditions for both rapid electrophoretic DNA transport and efficient hybridization reactions. Hybridization efficiencies of at least a factor of 10 relative to the next best known buffer, Cysteine, are achieved. Test data demonstrate an approximately 50,000 fold increase in hybridization efficiency compared to Cysteine.
CA002264780A 1996-09-06 1997-08-18 Methods and materials for optimization of electronic hybridization reactions Expired - Fee Related CA2264780C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US70826296A 1996-09-06 1996-09-06
US08/708,262 1996-09-06
PCT/US1997/014489 WO1998010273A1 (en) 1996-09-06 1997-08-18 Methods and materials for optimization of electronic hybridization reactions

Publications (2)

Publication Number Publication Date
CA2264780A1 true CA2264780A1 (en) 1998-03-12
CA2264780C CA2264780C (en) 2006-08-01

Family

ID=24845074

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002264780A Expired - Fee Related CA2264780C (en) 1996-09-06 1997-08-18 Methods and materials for optimization of electronic hybridization reactions

Country Status (9)

Country Link
EP (1) EP1019711A4 (en)
JP (1) JP4213216B2 (en)
KR (1) KR100591626B1 (en)
CN (1) CN1180248C (en)
AU (1) AU723564B2 (en)
BR (1) BR9712800A (en)
CA (1) CA2264780C (en)
NZ (1) NZ334314A (en)
WO (1) WO1998010273A1 (en)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6051380A (en) * 1993-11-01 2000-04-18 Nanogen, Inc. Methods and procedures for molecular biological analysis and diagnostics
US7582421B2 (en) 1993-11-01 2009-09-01 Nanogen, Inc. Methods for determination of single nucleic acid polymorphisms using a bioelectronic microchip
US6468742B2 (en) 1993-11-01 2002-10-22 Nanogen, Inc. Methods for determination of single nucleic acid polymorphisms using bioelectronic microchip
US6379897B1 (en) * 2000-11-09 2002-04-30 Nanogen, Inc. Methods for gene expression monitoring on electronic microarrays
US5964995A (en) * 1997-04-04 1999-10-12 Caliper Technologies Corp. Methods and systems for enhanced fluid transport
US6238909B1 (en) * 1999-05-04 2001-05-29 Motorola, Inc. Method and apparatus for obtaining electric field-enhanced bioconjugation
WO2001023884A1 (en) * 1999-09-27 2001-04-05 Monsanto Technology Llc Methods for determining oils in seeds
US7309581B2 (en) * 2000-11-01 2007-12-18 Sysmex Corporation Method of staining, detection and counting bacteria, and a diluent for bacterial stain
GB0205455D0 (en) 2002-03-07 2002-04-24 Molecular Sensing Plc Nucleic acid probes, their synthesis and use
US7153687B2 (en) 2002-08-13 2006-12-26 Hong Kong Dna Chips Limited Apparatus and methods for detecting DNA in biological samples
JP4464664B2 (en) * 2003-06-13 2010-05-19 独立行政法人理化学研究所 Biomolecule microarray substrate, biomolecule microarray, device and method for promoting interaction, and method for detecting interaction
US7314542B2 (en) * 2004-09-23 2008-01-01 Nanogen, Inc. Methods and materials for optimization of electronic transportation and hybridization reactions
KR100785011B1 (en) * 2006-04-07 2007-12-11 삼성전자주식회사 How to Increase Nucleic Acid Hybridization Specificity Using a Biion Compound
RU2315299C1 (en) * 2006-09-20 2008-01-20 Государственное научное учреждение Всероссийский научно-исследовательский институт пищевой биотехнологии Российской академии сельскохозяйственных наук Working electrolyte for determining ion composition of liquid media using capillary electrophoresis

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4936963A (en) * 1987-05-27 1990-06-26 Abbott Laboratories Polycationic buffers and method for gel electrophoresis of nucleic acids
US5188963A (en) * 1989-11-17 1993-02-23 Gene Tec Corporation Device for processing biological specimens for analysis of nucleic acids
US6017696A (en) * 1993-11-01 2000-01-25 Nanogen, Inc. Methods for electronic stringency control for molecular biological analysis and diagnostics
US5585069A (en) * 1994-11-10 1996-12-17 David Sarnoff Research Center, Inc. Partitioned microelectronic and fluidic device array for clinical diagnostics and chemical synthesis
DE69533554T2 (en) * 1994-11-10 2005-01-27 Orchid Biosciences, Inc. LIQUID DISTRIBUTION SYSTEM

Also Published As

Publication number Publication date
EP1019711A4 (en) 2001-06-13
KR100591626B1 (en) 2006-06-20
WO1998010273A1 (en) 1998-03-12
CA2264780C (en) 2006-08-01
NZ334314A (en) 2000-09-29
BR9712800A (en) 1999-11-23
CN1230255A (en) 1999-09-29
JP4213216B2 (en) 2009-01-21
AU723564B2 (en) 2000-08-31
CN1180248C (en) 2004-12-15
KR20010029477A (en) 2001-04-06
AU4071997A (en) 1998-03-26
EP1019711A1 (en) 2000-07-19
JP2001501301A (en) 2001-01-30

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