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CA2246259A1 - Genetic markers and methods for the detection of escherichia coli serotype-0157:h7 - Google Patents

Genetic markers and methods for the detection of escherichia coli serotype-0157:h7

Info

Publication number
CA2246259A1
CA2246259A1 CA002246259A CA2246259A CA2246259A1 CA 2246259 A1 CA2246259 A1 CA 2246259A1 CA 002246259 A CA002246259 A CA 002246259A CA 2246259 A CA2246259 A CA 2246259A CA 2246259 A1 CA2246259 A1 CA 2246259A1
Authority
CA
Canada
Prior art keywords
escherichia coli
detection
methods
genetic markers
identification
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002246259A
Other languages
French (fr)
Other versions
CA2246259C (en
Inventor
Mark Anton Jensen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
EIDP Inc
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2246259A1 publication Critical patent/CA2246259A1/en
Application granted granted Critical
Publication of CA2246259C publication Critical patent/CA2246259C/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

A method, diagnostic sequences and primers are provided that are useful in the identification of the Escherichia coli 0157:H7 serotype.
The method first involves the identification of a RAPD-amplified DNA fragment common to 0157:H7 Escherichia coli, the identification of the most conserved regions of that fragment, and the preparation of specific primers useful for detecting the presence of a marker within the fragment whereby that set of primers is then useful in the identification of all 0157:H7 Escherichia coli.
CA002246259A 1996-02-29 1997-02-24 Genetic markers and methods for the detection of escherichia coli serotype-0157:h7 Expired - Lifetime CA2246259C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US08/608,881 1996-02-29
US08/608,881 US5747257A (en) 1996-02-29 1996-02-29 Genetic markers and methods for the detection of escherichia coli serotype-0157:H7
PCT/US1997/002831 WO1997032043A1 (en) 1996-02-29 1997-02-24 Genetic markers and methods for the detection of escherichia coli serotype-0157:h7

Publications (2)

Publication Number Publication Date
CA2246259A1 true CA2246259A1 (en) 1997-09-04
CA2246259C CA2246259C (en) 2008-12-30

Family

ID=24438454

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002246259A Expired - Lifetime CA2246259C (en) 1996-02-29 1997-02-24 Genetic markers and methods for the detection of escherichia coli serotype-0157:h7

Country Status (8)

Country Link
US (1) US5747257A (en)
EP (1) EP0885310B1 (en)
JP (1) JP4212648B2 (en)
AT (1) ATE305051T1 (en)
AU (1) AU2135497A (en)
CA (1) CA2246259C (en)
DE (1) DE69734236D1 (en)
WO (1) WO1997032043A1 (en)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6037128A (en) * 1997-03-31 2000-03-14 Council Of Scientific & Industrial Research Process for the preparation of semisynthetic amplicon useful for sex determination of the papaya plant
US6180345B1 (en) 1998-03-31 2001-01-30 Counsel Of Scientific & Industrial Research Process for simultaneous preparation of sex specific and gender-neutral semisynthetic amplicons useful for sex determination
FR2777907B1 (en) * 1998-04-28 2002-08-30 Pasteur Sanofi Diagnostics NUCLEOTIDE SEQUENCES FOR THE DETECTION OF ESCHERICHIA COLI ENTEROHEMORRAGIQUES (EHEC)
NZ512088A (en) * 1998-12-08 2004-01-30 Children S Hospital And Region Polymorphic loci that differentiate escherichia coli 0157:H7 from other strains
FR2784117B1 (en) * 1999-07-07 2003-03-21 Pasteur Sanofi Diagnostics NUCLEOTIDE SEQUENCES FOR THE DETECTION OF ESCHERICHIA COLI ENTEROHEMORRAGIQUES (EHEC)
EP1409738A4 (en) * 2001-06-22 2005-03-30 Marshfield Clinic METHODS AND OLIGONUCLEOTIDES FOR THE DETECTION OF SALMONELLA SP., E. COLI O157: H7, AND LISTERIA MONOCYTOGENES
WO2003016546A1 (en) * 2001-08-21 2003-02-27 Flinders Technologies Pty Ltd. Method and device for simultaneously molecularly cloning and polylocus profiling of genomes or genome mixtures
WO2005113773A1 (en) * 2004-05-20 2005-12-01 Warnex Research Inc. Polynucleotides for the detection of escherichia coli 0157:h7 and escherichia coli 0157:nm verotoxin producers
US7309589B2 (en) 2004-08-20 2007-12-18 Vironix Llc Sensitive detection of bacteria by improved nested polymerase chain reaction targeting the 16S ribosomal RNA gene and identification of bacterial species by amplicon sequencing
KR100671501B1 (en) * 2005-02-28 2007-01-19 삼성에버랜드 주식회사 Primer for detecting food poisoning and method for detecting bacterial food poisoning using same
US20060246463A1 (en) * 2005-04-20 2006-11-02 Vevea Dirk N Methods and oligonucleotides for the detection of Salmonella SP., E coli 0157:H7, and Listeria monocytogenes
US20060240442A1 (en) * 2005-04-20 2006-10-26 Vevea Dirk N Methods and oligonucleotides for the detection of Salmonella SP., E coli 0157:H7, and Listeria monocytogenes
EP2004849A2 (en) * 2006-03-10 2008-12-24 Council of Scientific & Industrial Research A method for the screening of bacterial isolates
WO2010132832A1 (en) 2009-05-15 2010-11-18 Life Technologies Corporation E. coli o157:h7 specific assay
CN111575391A (en) * 2020-05-12 2020-08-25 浙江省检验检疫科学技术研究院 Identification method, kit and probe for detecting Escherichia coli O157: H7 by liquid phase in situ fluorescence hybridization

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5087558A (en) * 1981-09-25 1992-02-11 Webster John A Jr Method for identifying and characterizing organisms
US4717653A (en) * 1981-09-25 1988-01-05 Webster John A Jr Method for identifying and characterizing organisms
NL8900670A (en) * 1989-03-17 1990-10-16 Stichting Tech Wetenschapp POLYNUCLEOTIDE PROBE, METHOD AND KIT FOR IDENTIFYING AND DETECTING GRAM-NEGATIVE BACTERIA.
JP3258658B2 (en) * 1989-05-31 2002-02-18 ジーン−トラック・システムス Universal eubacterial nucleic acid probe and method
US5126239A (en) * 1990-03-14 1992-06-30 E. I. Du Pont De Nemours And Company Process for detecting polymorphisms on the basis of nucleotide differences
DE69128545D1 (en) * 1990-08-24 1998-02-05 Univ Tennessee Res Corp GENETIC FINGERPRINT TECHNIQUE WITH DNA REPLACEMENT
WO1992007095A1 (en) * 1990-10-15 1992-04-30 Stratagene Arbitrarily primed polymerase chain reaction method for fingerprinting genomes
WO1992007948A1 (en) * 1990-11-06 1992-05-14 The Lubrizol Corporation Compositions and methods for analyzing genomic variation
CA2055302A1 (en) * 1990-11-15 1992-05-16 Roger G. L. Wheatcroft Specific microbial strain detection method
US5437975A (en) * 1991-02-25 1995-08-01 California Institute Of Biological Research Consensus sequence primed polymerase chain reaction method for fingerprinting genomes
EP0517361A1 (en) * 1991-05-30 1992-12-09 Amoco Corporation A method for detecting and identifying pathogenic organisms using target sequences as detectors
EP0517154A1 (en) * 1991-06-06 1992-12-09 F. Hoffmann-La Roche Ag Method for detecting giardia
CA2077135A1 (en) * 1991-08-30 1993-03-01 Joh-E Ikeda A method of dna amplification
US5340728A (en) * 1992-12-09 1994-08-23 E. I. Du Pont De Nemours And Company Method for amplification of targeted segments of nucleic acid using nested polymerase chain reaction
US5660981A (en) * 1994-06-06 1997-08-26 E. I. Du Pont De Nemours And Company Selection of diagnostic genetic markers in microorganisms and use of a specific marker for detection of salmonella
US5475098A (en) * 1994-06-14 1995-12-12 The United States Of America As Represented By The Department Of Health And Human Services Distinctive DNA sequence of E. coli 0157:H7 and its use for the rapid, sensitive and specific detection of 0157:H7 and other enterohemorrhagic E. coli
US5654417A (en) * 1995-04-14 1997-08-05 Children's Hospital And Medical Center Nucleic acid probes for detecting E. coli O157:H7

Also Published As

Publication number Publication date
US5747257A (en) 1998-05-05
EP0885310B1 (en) 2005-09-21
ATE305051T1 (en) 2005-10-15
JP4212648B2 (en) 2009-01-21
DE69734236D1 (en) 2005-10-27
JP2000511767A (en) 2000-09-12
AU2135497A (en) 1997-09-16
CA2246259C (en) 2008-12-30
WO1997032043A1 (en) 1997-09-04
EP0885310A1 (en) 1998-12-23

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Effective date: 20170224