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CA2245675A1 - Engineering affinity ligands for macromolecules - Google Patents

Engineering affinity ligands for macromolecules

Info

Publication number
CA2245675A1
CA2245675A1 CA 2245675 CA2245675A CA2245675A1 CA 2245675 A1 CA2245675 A1 CA 2245675A1 CA 2245675 CA2245675 CA 2245675 CA 2245675 A CA2245675 A CA 2245675A CA 2245675 A1 CA2245675 A1 CA 2245675A1
Authority
CA
Canada
Prior art keywords
target
binding
ligands
release
preselected
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA 2245675
Other languages
French (fr)
Other versions
CA2245675C (en
Inventor
John M. Maclennan
Robert C. Ladner
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Dyax Corp
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Priority claimed from PCT/US1997/004425 external-priority patent/WO1997035196A1/en
Publication of CA2245675A1 publication Critical patent/CA2245675A1/en
Application granted granted Critical
Publication of CA2245675C publication Critical patent/CA2245675C/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

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  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

A method is disclosed for obtaining highly specific and tailored ligands suitable for purifying a particular product target or for eliminating particular target impurities in a feed stream. Engineered affinity ligands according to the invention will bind a target with high specificity at a preselected binding condition and release the target at a preselected elution condition. The ligands are isolated by contacting a target with a multiplicity of polypeptides derived through variegation of the structure of a candidate binding domain, the variants (or analogues) including polypeptides favoring binding to the target under desired binding conditions and release from the target under elution conditions, where the binding and elution conditions differ according to one or more parameters, such as pH, temperature, concentration of salt or volume % of an organic solvent.
CA 2245675 1996-03-20 1997-03-20 Engineering affinity ligands for macromolecules Expired - Lifetime CA2245675C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US61988596A 1996-03-20 1996-03-20
US08/619,885 1996-03-20
PCT/US1997/004425 WO1997035196A1 (en) 1996-03-20 1997-03-20 Engineering affinity ligands for macromolecules

Publications (2)

Publication Number Publication Date
CA2245675A1 true CA2245675A1 (en) 1997-09-25
CA2245675C CA2245675C (en) 2008-02-19

Family

ID=29422946

Family Applications (1)

Application Number Title Priority Date Filing Date
CA 2245675 Expired - Lifetime CA2245675C (en) 1996-03-20 1997-03-20 Engineering affinity ligands for macromolecules

Country Status (1)

Country Link
CA (1) CA2245675C (en)

Also Published As

Publication number Publication date
CA2245675C (en) 2008-02-19

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