CA2232514A1 - Method of transgene expression and secretion in schistosoma - Google Patents

Abstract

This invention is a method of producing schistosomes as an intermediate transgene vector for secretion of desired gene products. The desired products are secreted into the bloodstream of the host (patient) by schistosomes that have been engineered through the germline with DNA encoding the transgene. The use of schistosomes as an intermediate vector facilitates mass production, quality control, termination of therapy at will and dose titration. The method is applicable to situations in which the acquired protein is functional in the plasma or in endocytotic vesicles.

Description

CA 02232~14 1998-03-19 W O 97/11191 PCTrUS96tl5083 OF TRANSGENE EXPRESSION AND SECRETION IN SCHISTOSOM~

~A~-~oun~D
This invention relates to the field of gene therapy.
Most current strategies of gene therapy employ mechanisms to alter the patient's own cells to produce the desired gene product, through viral and non-viral vectors that introduce DNA that encodes the desired product. Some of the major pitfalls of such methods are: low e~ficiency of introduction and expression, potential for viral infection by cont~m;n~ting replication competent virus, potential for recombination with host DNA and for promoting malignant transformation, irrever-sibility of the process, and the need for labor-intensive individualized treatment. The method described here avoids these problems because it uses an intermediate vector for gene expression in the patient, a vector that can be mass- produced and batch-characterized, that can be eliminated at will and that does not alter the DNA of the patient's own cells.
This patent describes a method of creating genetically engineered schistosomes as a vector for secretion of therapeutic proteins into the bloodstream of humans and other susceptible hosts. This process will result in a sustained in vivo protein expression system. This system avoids the need for large scale protein purification and for repeated injections of therapeutic proteins that must be administered parenterally, such as insulin or erythropoietin. This mode of protein expression is a form of '~gene therapy" applicable in situations where the gene introduced, hereafter referred to as the "transgene", does not require expression in the cells of the patient but rather can be functionally expressed in an intermediate, symbiotic vector.
Humans are hosts to seven schistosome species, the blood flukes (Rollinson D and Southgate VR, The Genus Schistosoma: A
Taxononmic Appraisal. pp. 1-49 in D. Rollinson, AJG Simpson, Eds., The Biology of Schistosomes from Genes to Latrines, London Academic Press, 1987, reviewed in Basch PF, Overview, pp. 3-33, in Schistosomes: Development, Reproduction, and Host Relations, PF Basch, New York, Oxford University Press, Inc., = = ~ -CA 02232~14 1998-03-19 W O 97/11191 PCT~US96/15083 1991). Schistosomes are parasites ~n~m~c in many third world countries where poor sanitation and m~nll~l farming practices ~ allow perpetuation of the species through their various life cycle stages. These stages include the sporocyst forms that grow in fresh water snails and the adult worms that develop and mate within the human blood stream. The adult worms lay eggs in the wall of the bladder or intestine, most of which are eventually expelled though the stool or the urine. The mechanisms that allow the adult worms to evade the human host's immune response and to survive in the bloodstream for many years without causing symptoms are not completely understood. However, this ability makes them a useful vector for delivery of therapeutic proteins into the human blood-stream. Infection with schistosomes is not innocuous, though, for, in individuals who harbor many parasites, a characteris-tic disease ensues caused by the body's response to eggs that fail to get expelled. The retained eggs ~low though the veins and provoke a granulomatous response leading to fibrosis of tissues where they are deposited: the liver, for species that live in the portal veins (e.g. S. mansoni ), or the bladder wall, for species that live in the pelvis (e.g. S. haematobium) .
To avoid this problem in recipients of the schistosome vector for protein expression, this patent deals with the creation of transgenic schistosomes which lay soft, degradable eggs or which lay eggs with reduced sclerotin content.
It is desirable to provide a wide variety of possible transgene products for insertion into the vector. An exhaustive list of potential products for expression in this system is not intended. Theoretically, the system described herein is suitable for expression of any protein that is active in the plasma or that can be targeted from the bloodstream to its appropriate extracellular or intracellular location. Several categories of proteins suitable for this therapeutic system are noted below, with examples given for each.
It is desirable to provide for the insertion of protein and glycoprotein hormones, such as:
- insulin: Although blood-glucose-level-regulated-expression of insulin is required for proper glucose control, CA 02232~14 1998-03-19 W O 97/11191 PCT~US96/15083 a constant, low-level baseline expression o~ insulin may prove to be extremely valuable for preventing hyperglycemic episodes leading to ketoacidosis. In addition, low level baseline expression may reduce the number of daily injections needed for many insulin-dependent diabetics.
- leptin: This newly discovered adipocyte hormone is sure to play a role in body fat regulation for many individuals. Probably, only low levels of expression are required for therapeutic benefit, and it does not require a timed expression pattern.
- calcitonin: Osteoporosis is a major cause of morbidity and mortality among post-menopausal women, the elderly and steroid-dependent individuals. Calcitonin injections are one mode of therapy used ~or such individuals. A boost in baseline calcitonin levels using this vector may replace that mode of therapy.
It is also desirable to provide for the insertion of non-hormonal circulating proteins, such as:
- alpha-1-anti-trypsin: Deficiency of this plasma protein causes significant disease in 1/3500 individuals, leading to cirrhosis as well as emphysema. Constant low levels of expression are required to prevent tissue destruction.
- factor VIII: Deficiency of this protein causes hemophilia in 1/10,000 males. Constant low serum levels are required to prevent morbidity.
- cholesterol ester transfer protein inhibitor:
Aberrant lipoprotein profiles are a significant cause of morbidity from atherosclerosis. Agents acting to increase HDL/LDL cholesterol, such as a peptide designed to inhibit this enzyme, may have a tremendous effect on disease in individuals at risk.
- human immunodeficiency virus co-receptor ligands:
Recently, co-receptors for HIV on T-cells (the SDF-l chemokine receptor, LESTR/fusin) and macrophages (the beta-chemokine receptor, CC-CKR) have been identified. Individuals with elevated levels of beta-chemokines are resistant to HIV
infection. Artificially raising serum levels of the ligands CA 02232~14 1998-03-19 W O97/11191 PCTrUS96/15083 for these receptors may protect against infection with HIV or slow disease progression.
In addition, it is desirable for this method to be used against lysosomal storage diseases, for example, for the insertion of beta-glucocerebrosidase, deficiency of which causes Gaucher's disease, most common in Ashkenazic Jews.
Exogenous administration of the purified enzyme from placenta is potentially curative, as the protein is targeted to the lysosomal compartment. Therapy is presently limited by availability of enzyme, which must be repetitively injected.
It is further desirable to combat borderline deficiency protein states contributory to disease, for example, with regard to deficiency of CRl. In collagen vascular diseases triggered by immune complexes (e.g. S~E), erythrocyte complement receptor levels are reduced, leading to delayed clearance of circulating immune complexes and deposition in tissues, with ensuing glomerulonephritis or vasculitis.
Bxpression of a soluble CRl receptor might facilitate clearance of immune complexes by the reticuloendothelial system and prevent relapses.
Furthermore, many diseases are caused by defects or deficiencies of intracellular non-lysosomal (e.g. cytosolic, mitochondrial) enzymes or structural proteins. Methods may be developed in the future to allow postranslational transmem-brane passage of desired proteins, possibly based upon theparadigm of the dimeric ricin and diphtheria toxins. Then the schistosome expression vector could be used to deliver proteins to treat glycogen storage diseases, hormone receptor defects, and many metabolic disorders requiring replacement of a cytosolic or even possibly subcellularly localized (not only lysosomal) protein.
Moreover, proposals for therapy based upon novel protein constructs herald the next phase of therapeutic advances such as expression of novel inhibitor proteins, hybrid proteins, magic bullet proteins, etc. These ideas include combinations of antibody variable region domains and toxins to kill cells bearing specific antigens, juxtaposition of antigens with negatively signaling molecules to induce tolerance, and dummy viral receptors (e.g. CD4, to fight HIV.) CA 02232~14 1998-03-19 WO 97/11191 PCTrUS96/15083 STTMM~Y OF TEE lNY~N-llON
In general, the invention is a transgenic schistosome, male or female depending on the particular embodiment, whose genome has been stably transformed by DNA encoding a transgene within appropriate regulatory contexts. The transgenic schistosome secretes the transgene product into the blood-stream of its human or other definitive host. The invention takes advantage of developed methods for propagating schistosomes in snails at the sporocyst life cycle stage in order to obtain large clonal populations of recombinant schistosomes. The method adapts existing technology that has been developed for microinjection of eggs of other species for use in the injection of schistosome eggs, based upon known aspects of schistosome biology.
The invention includes methods to use male schistosomes as vectors in unisexual infections and to use female schistosomes in bisexual infections. In female infections, methods to decrease egg production and eggshell maturation are described. The invention uses specific types of DNA
constructs encoding antisense RNAs and ribozymes to interfere with schistosome eggshell protein production or maturation either directly or by interfering with the action of tissue-specific transcription factors, and the invention describes methods to clone these transcription factors and eggshell tanning enzymes. The invention employs use of schistosome genomic DNA locus control region-like elements to con~er high level tissue-specific expression of the transgene, based upon work done in the mouse, and it describes how to identify and utilize these regulatory regions for creation of the transgene construct. The invention utilizes DNA
constructs encoding various mRNA regulatory sequences and signal peptides based upon published schistosome and non-schistosome sequences. The invention describes strategies for mating recombinant schistosomes to obtain the most ef~ective transgenic schistosome vector.

BRIEF DESCRIPTION OF THE DRAWINGS
Figure 1 is a flow-chart of the method for obtaining clones of recombinant schistosome clones;

CA 02232~14 1998-03-l9 W O97/11191 PCT~US96/15083 Figure 2 is a diagrammatic representation of how to adapt-endogenous schistosome gene transcriptional regulatory sequences for use in the transgene vectori Figure 3 is a diagrammatic flow-chart of how to obtain female transgenic clones on an eggshell knockout background;
and Figure 4 is a diagram of the transcriptional regulatory regions to accompany the various transcripts of the multiple DNA constructs to be used in 2-step and 3-step schistosome genetic modifications.

DET~TT~n DESCRIPTION OF T~E lNv~NllON
I. General Considerations:
One way to use schistosomes as expression vectors without causing granulomatous disease is to treat patients with only male worms, so that no granuloma-promoting eggs are laid. In species that infect hl~m~nq, the male is more robust than the female, which is dependent upon the male for transport through the venous plexuses that they inhabit (Standen OD, The Relationship of Sex in Schistosoma mansoni to Migration Within The Hepatic Portal System of Experimentally Infected Mice, Annals of Tropical Medicine and Parasitology 47:139-145, 1953, reviewed in Basch PF, Sexual and Conjugal Biology, pp.137-171, Basch PF, 1991). However, male worms of some species mature in the absence of females (S. Mansoni; Vogel H, 1941 Infektions-versuche an verschiedenen Bilharzia-Zwischenwirten mit einem einzelnen Mirazidium von Bilharzla mansoni und B.
japonica Zentrallblatt fur Bakteriologie Abteilung I.
Originale 148: 29-35, S. Haematobium; Sahba GH and Malek EA, 1977 Unisexual infections with Schistosoma haematobium in the mouse, American Journal of Tropical Medicine and Hygiene 26:331-33, reviewed in Basch PF, 1991, Pp 137-71), and migrate almost completely to the appropriate location (i.e., the portal vein for S. Mansoni; St~n~n, 1953, and Armstrong JC, Mating Behavior and Development of Schistosomes in the Mouse, Journal o~ Parasitology 51:605-15, 1965) enabling use of male worms as vectors in unisexual in:Eections.
In order to take advantage of male vectors in unisexual infections, the protein expression is targeted to the worm's CA 02232~14 1998-03-l9 WO 97/11191 PCT~US96/15083 integument. Although the integument is not specialized ~or protein secretion, it nonetheless has a tremen~ous metabolic capacity for surface membrane protein production, a pathway to which exogenous proteins are targeted in this invention. The schistosome integument is a multi-laminate membrane (Silk MH
et al., Ultrastructural studies of the blood ~1uke Schistosoma mansoni I, The integument, South Afrikan Journal of Medical Sciences 34:1-10, 1969; Hockley DJ and McLaren DJ, Schistosoma mansoni: changes in the outer membrane of the tegument during development from cercaria to adult worm, International Journal for Parasitology 3:13-25, 1973; Torpier G, Capron M and Capron A, Structural changes of the tegmental membrane complex in relation to developmental stages of Schistosoma mansoni (Platyhelminthes: trematoda). Journal of Ultrastructural Research 61:309-24, 1977), that serves as a barrier to the host's immune system. Proteins targeted for secretion in this location should eventually find their way to the worm exterior, either after ~usion of secretory vesicles with the exterior leaflet or after sloughing of the exterior leaflet, with release of material from the intermembranous space.
Transgene expression targeting to schistosome tissues other than the integument may, in fact, prove to be more efficacious. Structures that normally actively secrete soluble proteins are obvious targets ~or expression. These structures include the gut, the vitelline gland, the Mehlis gland and the ootype. In the gut, digestive enzymes (Gotz B and Klinkert M, Expression and partial characterization o~ a cathepsin B-like enzyme (Sm31) and a proposed ~haemoglobinase' (Sm32) from Schistosoma mansoni, Biochemistry Journal, 290:801-06, 1993) will most likely prevent significant amounts of intact exogenous protein from reaching the bloodstream, and any attempt to inactivate these enzymes will probably impair worm viability. The other structures mentioned are part of the female genital tract and are specialized ~or protein secretion, evidenced by their glandular structure and = secretory epithelium, with ultrastructurally prominent Golgi apparatus and secretory vesicles (Spence IM and Silk MH, Ultr~structural studies o~ the blood ~luke- Schistosoma mansoni: V, the female reproductive system- a prel- m; n~y CA 02232~14 1998-03-l9 W O 97/11191 PCT~US96/15083 report, South A~rican Journal o~ Medical Sciences, 36:41-50, 1971; Spence IM and Silk MH, Ultrastructural studies of the blood fluke- Schistosoma mansoni VI, the Mehlis gland, South African Journal of Medical Sciences, 36:41-50, 1971).
However, because o~ the ~emale's dependence on contact with the male for maturation, targeted expression of exogenous proteins in these tissues o~ female worms requires bisexual infections in order to be utilized for human therapy, with the ensuing possibility of granuloma formation.
The unwanted side effect of egg production would be clinically relevant only if many worm pairs were to be required for many years, as only about 5-10~ of infected individuals develop severe illness. For short term treatment, or if adequate levels of transgene product can be obtained with only a few worm pairs, then egg laying may not be a significant clinical problem when weighed against the severity of the disease being treated.
In strategies targeting transgene expression to female organs, several methods are described to prevent release of viable eggs in order to avoid causing granulomatous disease.
One possible solution is, again, to use male-only infections, as some development of the female reproductive glands (vitelline or Mehlis glands) does take place even in male worms. These genotypically male partial hermaphrodites have been found in unisexual male infections and never result in egg formation in schistosome species that infect hllm~nq (Shaw MK and Erasmus DA, Schistosoma mansoni: The Presence and Ultrastructure of Vitelline Cells in Adult Males, Journal of Helminthology 56:51-53, 1982; reviewed in Hermaphraditism in Male Schistosomes, pp 162-164, in Ch. 4, Sexual and Conjugal Biology, in Schistosomes, PF Basch, 1991). Despite the possible use of hermaphrodite males, full utilization of the powerful secretory system of the female reproductive system most likely requires expression in ~emales. For expression in the most abundant and active female tissue, the vitelline gland, strategies are described below to interfere with the female~s ability to make eggshell protein and enzymes involved in eggshell hardening.

CA 02232~14 1998-03-19 W O 97/11191 PCTrUS96/15083 II. General methods of schistosome propaqation, transformation and cloninq Isolates of schistosomes, including S. mansoni, S.
haematobium and S. japonicum are obtained ~rom stool (or urine ~or S. Haematobium) ~rom in~ected hl~m~n~ or ~rom established laboratory strains, and passaged in susceptible Biomphalaria glabrata (or Bulinus ~or S. Haemato~ium) snails and susceptible mouse strains or hamsters (see table 2-3 in Basch, 1991, and re~erences therein) as described (Hacket F, The culture o~ Schistosoma mansoni and production of li~e cycle stages, in Methods in Molecular Biology, vol. 21:
Protocols in Molecular Parasitology, JEH Xyde ed., Humana Press Inc, Totowa New Jersey, 1993; MacInnis AJ, Maintenance o~ Schistosoma mansoni and Schistosomatium douthitti, in Experiments and Techniques in Parasitology, MacInnis A and Voge M, Freeman Press, 1970; Smithers SR and Terry RJ, The in~ection o~ laboratory hosts with cercaria of Schistosoma mansoni and the recovery o~ the adult worms, Parasitology 55:695-700, 1965).
Schistosome eggs must ~irst be isolated ~or microinjec-tion. The protocol ~or creating transgenic schistosomes is shown in Figure 1. Freshly laid (stage I) eggs, containing oocytes undergoing meiosis and male pronuclei, are harvested by microdissection ~rom the intestines of schistosome-in~ected laboratory animals (Box 1), such as mouse, hamster or guinea pig (Pellegrino et al , 1962, Pelligrino and Faria, 1965).
In~ected animals are sacri~iced in the week prior to the ~orty-~ourth day post-infection, when immature eggs predominate (Pelligrino J et al., New approach to the screening o~ drugs in experimental schistosomiasis mansoni in mice, American Journal o~ Tropical Medicine and Hygiene 11:201-15, 1962; Pelligrino J and Faria J, The oogram method ~or the screening o~ drugs in schistosomiasis mansoni, American Journal o~ Tropical Medicine and Hygiene 14:363-69, 1965) and are identi~ied in squash preparations o~ small intestines as described (ibid, incorporated herein by re~erence). Eggs in the same developmental stage are ~ound in clusters within a venule, and stage can be visually assessed in a dissecting microscope based upon the cellularity o~ the CA 02232~l4 l998-03-l9 W O 97/11191 PCT~US96/15083 eggs (box 2) (ibid). Those in stage I are dissected away from-intestinal tissue and placed sterily into a chamber for microinjection containing cell culture medium (see below) with 25 mM HEPES (pH 7.4) in place o:E bicarbonate. Collaginase treatment is performed at this point, i~ necessary, to remove adherent mouse tissue. Approximately 50 stage I eggs are removed from each maximally infected ~n~m~l.
Older eggs have a dense eggshell coat that may prevent easy introduction of a microinjection needle. In addition, nuclear or cytoplasmic enzymes required for recombination may be present exclusively during early zygotic period.
Identifying this early egg stage is also necessary because later stages, passed in the stool, contain multicellular developing miracidial organisms, injection o~ which may be 15 more difficult and may lead to a non-clonal distribution of DNA integration in progeny, complicating the analysis of transgenics.
An alternative method for obtaining early stage eggs for microinjection is to isolate eight-week old adult 20 schistosomes, culture them in vitro, and recover the earliest laid eggs from the culture medium as soon as possible. The techni~ue for recovering adult schistosomes and the optimized parameters for culture of eggs to reach maturity in vi tro have been assessed in detail (Newport G and Weller TH, 1982.
25 Miricidia infective for snails derived from eggs laid by adult Schistosoma mansoni in vitro, Parasitology 84:481-9O, 1982;
Newport G and Weller TH, Deposition and maturation of eggs of Schistosoma mansoni in vitro: importance of fatty acids in serum-free media, American Journal of Tropical Medicine and 30 Hygiene, 3:349-57, 1982; WU GY et al., Stimulation of Schistosoma mansoni oviposition in vitro by ~n~m~l and human portal serum, American Journal of Parasitology 74:618-22, 1982, all incorporated herein by reference).
Conditions i~or DNA microinjection (Box 3) are based upon 35 optimized protocols from mouse egg microinjection (Brinster RL
et al., Factors affecting the efficiency of introducing foreign DNA into mice by microinjecting eggs, Proceedings of the National Academy o~ Sciences, 82:4438-42, 1985), i~or which an integration efficiency of 27.1~ has been achieved. The CA 02232~14 1998-03-19 W O 97/11191 PCT~US96/15083 schistosome zygotic nuclei are approximately 6 to 8 microns in-diameter (Nez MM and Short RB, Gametogenesis in Schistosomatium douthitti (Cort)(Schistosmatidae: Trematoda), Journal o~ Parasitology 43: 167-82, 1957; Neill PJ et al., The ultrastructure of the Schistosoma mansoni egg, American Journal o~ Tropical Medicine and Hygiene 60: 429-30, 1988) slightly smaller than those of the mouse. Thus, up to one hal~ the volume used by Brinster, up to lpL o~ purified linear DNA with staggered ends are injected into the ~emale or male pronucleus at a concentration of 1-2ng/mcL (50-100 copies o~ a lOkb sequence) in lOmM TrisCl (pH 7.5) 0.25mM EDTA. DNA
concentration, volume injected and presence o~ cytochalasin B
(a mitotic inhibitor) in the egg incubation medium are varied to optimize egg survival and DNA integration e~iciency in initial experiments. Microinjection is per~ormed with eggs placed under silicone oil on commercially-available depression slides or laboratory-prepared dried agarose coated glass cover slips, using standard techniques. Nuclear injection is con~irmed in parallel control samples by using the nondi~usible dye FITC-dextran to ~ollow injections with ~luorescence microscopy as has proven use~ul in microinjection o~ other helminths (Fire A, Integrative Trans~ormation of Caenorhabditis elegans, The EMBO Journal 5:2673-80, 1986).
For miracidial maturation (Box 4) injected eggs are maintained at 37~C in 5~ CO2in bicarbonate bu~ered cell culture medium (see Newport and Weller, 1982, Parasitology, re~erenced above, ~or schistosome egg culture technique) with addition o~ ~etal cal~ serum and casein hydrolysate ~or approximately six days, until the miracidia reach maturity.
Under the conditions reported, a workable proportion o~ the eggs reach maturity (approximately 12~) see table 1 (casein hydrolysate and mouse r.b.c.) see table 2 (8~ ~etal cal~ serum in DSMH), and their ability to productively in~ect snails was proven (ibid). Miracidial hatching and infection (Box 5) are per~ormed by placing individual mature eggs in small beakers containing a small amount o~ spring water and a single snail and exposing to bright light ~or ~ive minutes as described (MacInnis AJ, 1970). In~ected snails are then reared together until miracidia are produced (Box 6).

CA 02232~14 1998-03-19 Propagation and analysis o~ recombinant clones (Boxes 7-11) is done pre~erably as ~ollows: Because the released miracidia are used to infect susceptible snails on a one miracidium per snail basis, each snail releases thousands o~
genetically identical cercaria, which constitute a schistoso-mal clone. Analysis o~ infected snails is conducted weekly a~ter the third week to determine which are productively in~ected. As described (McInnis AJ, 1970; Hyde JE, 1993, see above), snails, reared in darkness, are exposed to light ~or 60 minutes in deionized water, and the released cercaria are killed, stained with Lugol's iodine and counted microscopic-ally in lOmcL aliquots (Box 7). Several hundred to thousands o~ cercaria are released per snail. In order to identi~y transgenic clones ~rom among productive in~ections, aliquots o~ lO0 cercaria are centri~uged and subjected to PCR analysis using primers speci~ic ~or transgene sequences (Box 7, A).
Southern blot analysis is per~ormed on the positive clones in order to eliminate clones carrying the transgene as an extrachromosomal array, which is not expected to be stably maintained due to the lack o~ centromeres. (For analysis o~
this phenomenon in another helminth (C. elegans), see Stinchcomb DT et al., Extrachromosomal DNA Trans~ormation o~
Caenorhabditis Elegans, Molecular and Cellular Biology 5:3484-96, 1985) Extrachromosomal arrays are identi~ied by the absence o~ higher molecular weight bands that correspond to segments o~ schistosome genomic DNA ~lanking the integra-tion site revealed by Southern blotting after digestion with a unique restriction site within the transgene, and by ~ast migration during electrophoresis o~ undigested DNA prepara-tions (Box 7, A). Karyotyping o~ recombinant schistosomestrains is per~ormed on interphase chromosomes ~rom cercaria.
Males have homotypic (ZZ) sex chromosomes and are easily recognizable by a large heterochromatin ~ragment (Grossman AI
et al., Sex heterochromatin in Schistosoma mansoni, Journal o~
Parasitology 66:368-70, 1980) (Box 7, B). Alternatively, PCR
directed to the repetitive pW1 element can be used ~or schistoBom manBoni~ (Webster P, Mansour TE, Bieber D, Isola-tion of a ~emale-speci~ic highly repeated Schistosoma mansoni DNA probe and its use in an assay o~ cercarial sex, Molecular CA 02232~l4 l998-03-l9 WO 97/11191 PCTrUS96/15083 and Biochemical Parasitology 36:217-22, 1989; Gasser RB, Morahan G and Mitchell GF, Sexing single larval stages of Schistosoma mansoni by polymerase chain reaction, Molecular and Biochemical Parasitology 47:2S5-58, 1991, both incor-porated herein by reference) (Box 7, B).
Clonal populations of sporocysts are maintained by serial passage in snails in order to ensure preservation of primary lines during further characterization of adults, as described (Box 8) (Cohen LM and Eveland LK, Schistosoma mansoni: Long-term maintenance of clones by microsurgical transplantation of sporocysts, 1984; Chernin E, Transplantation of larval schistosoma mansoni from infected to uninfected snails, The Journal of Parasitology 52:473-82, 1966, both incorporated herein by reference). Propagation by sporocyst transfer eliminates the need to follow the transgene segregation in ~urther generations and permits salvage of potentially sterile schistosome clones. This method has been described in detail (Cohen LM and Eveland LK, 1984). Brie~1y, hepatopancreas and ovotestis are isolated from snails four weeks after onset of cercarial prQductiQn, ~laced in ~bernln'~ B~ ~Che;Enin F, 1963), cut into narrow strips, and inserted through a puncture made with a 26 gauge needle into the cephalopedal sinuses of ten to twenty recipient snails. Cohen and Eveland reported transfer to 87~ of surviving (56~) recipients. Thus, serial sporocyst transfer to ten snails should be sufficient to insure propagation o~ 99.9~ of all clones in each round.
Repeat Southern blot analysis of transgenic clones is per~ormed after three to ~ive rounds of sporocyst transfer to confirm stabile heritability of the transgene (Box 9). Adult worms are obtained in mixed bisexual infections of laboratory animals with transgenic cercarial clones and with wild type cercarial clones of the opposite sex (Box 10). Alternatively, cercaria are raised to adulthood in culture (Basch, 1981a and 1981b). Assay ~or release of desired protein into the host's bloodstream or culture medium is performed by an appropriate technique, such as ELISA, radioimmunoassay, or a specific ~ bioassay or a chemical assay (Box 11). Pilot studies utilize human growth hormone as a reporter gene, which has a well-characterized and sensitive assay system (Selden RF et al., CA 02232~14 1998-03-19 W O 97/11191 PCT~US96/15083 Molecular and Cellular Biology 6:3173-79, 1986). Immunohisto-chemistry and in situ hybridization are also per~ormed in order to confirm the location o~ expression within the worms.

Breeding o~ transgenic clones (Box 12) may be necessary to ultimately obtain high-copy number clones of the appro-priate sex. For clones secreting from the integument, male cercaria are desired ~or human subject in~ection and are easily obtained in bulk ~rom the appropriate male transgenic schistosome clones maintained in snails by sporocyst trans~er.
I~ the primary clone is ~emale and ~ertile, then, to take advantage o~ this recombinant, a male transgenic clone must be selected ~rom among the o~spring o~ this clone a~ter breeding with normal males by co-in~ection o~ the laboratory ~nim~l host. Through additional crossbreeding o~ independent transgenic clones, progeny carrying multiple copies of the desired DNA construct can be identi~ied, with a potentially dose-related increase in protein secretion. Likewise, ~or clones with expression targeted to ~emale-speci~ic secretory structures, ~emale transgenic cercaria must be similarly obtained.

III Desiqn of the Transqenic DNA Vector Construct A. Local Promoter Elements The injected transgene vector contains the cDNA sequence o~ the desired transgene within the DNA context required to direct a high level o~ tissue-speci~ic expression, and the cDNA contains the signal sequences necessary to speci~y protein secretion. Figure 2A shows how genomic sequences ~rom a model schistosome gene (I) are used to produce the plasmid DNA construct containing the transgene (II). In this strategy, genomic sequences are incorporated en bloc. The upstream (a) and downstream (b) schistosome genomic ~ragments adopted to ~lank the transgene are each approximately 3.5 kb long, to include local promoter and enhancer sequences. The model schistosome gene chosen has the desired pattern o~
tissue-speci~ic expression in the integument, in the vitelline cells, in the Mehlis gland or in the ootype. To con~irm that no other schistosomal genes are contained within these CA 02232~14 1998-03-19 W O 97/11191 PCT~US96/15083 flanking sequences, Northern blot analysis of schistosome RNA
from all tissues and life cycle stages is performed using the flanking sequences as a probes, and open reading frames are found within the flanking sequences by DNA sequencing.
Also adopted into the transgene construct are 5' (c) and 3' (d) untranslated regions of the model gene, to promote proper post-transcriptional and post-translational processing within the schistosome target location. This includes trans-splicing (Rajkovic A et al., A spliced leader is present on a subset of mRNAs from the human parasite Schistosoma mansoni, Proceedings of the National Academy of Sciences (USA) 87:8879-83, 1990), and intracellular tra~icking to secretory pathways. The model gene coding sequence (e) is replaced by the transgene cDNA coding region (f). No intron is included in the transgene transcript (g) since most schistosome genes are intronless. The amino-terminal signal peptide is derived from either the model gene or from the transgene. However, if the transgenic products are found to be expressed in schisto-some cells but not secreted, then endogenous schistosome (export) signal sequences, as putatively identified (Hawn TR, Tom TD and Strand M, Molecular Cloning and Expression of SmIRV1, a Schistosoma mansoni antigen with similarity to Calnexin, Calreticulin, and OvRall, The Journal of Biological Chemistry, 268:7692-98, 1993, Chen L, Rekosh DM, LoVerde PT, Schistosoma mansoni p48 eggshell protein gene: characteriza-tion, developmentally regulated expression and comparison to the pl4 eggshell protein gene, Molecular and Biochemical Parasitology 52: 39-52, 1992) may be used in the construct exclusively. However, it is likely that these sequences show enough evolutionary conservation to function heterologously.
As described below, further tailoring of the vector is performed to include higher order regulatory elements and to eliminate unnecessary sequences. The DNA vector is propagated within a bacterial plasmid. Plasmid sequences are not microinjected into schistosome eggs and are removed from the transgene vector portion of the plasmid construct by restriction digestion at rare cutting ~n~onllclease cloning sites (h, h') engineered into the plasmid. Following digestion at these sites, the DNA fragments are separated by CA 02232~14 1998-03-19 W O97/11191 PCT~US96/15083 agarose gel electrophoresis or gel ~iltration and puri~ied by standard techniques.
Appropriate schistosomal model genes are those o~ highly-expressed tissue-speci~ic genes. For example, to target the exogenous protein to the integument, the regulatory regions of tegmental antigen genes such as Sml5.9 (Abath F GC et al., Structure o~ the gene encoding a putative Schistosoma mansoni tegumental antigen precursor, Molecular and Biochemical Parasitology 60:81-92, 1993), Sm21.7 (Francis P and Bickle Q, Cloning of a 21.7 kDa vaccine-dominant antigen gene o~
Schistosoma mansoni reveals an EF hand-like moti~, Molecular and Biochemical Parasitology 50:215-24, 1992), Sm22.6 (Je~s SA et al., Molecular cloning and characterisation o~ the 22-kilodalton adult Schistosoma mansoni antigen recognised by antibodies ~rom mice protectively vaccinated with isolated tegumental surface membranes, Molecular and Biochemical Parasitology 46:159-68, 1991), or o~ the glucose transporter genes, the SGTP's (Skelly PJ et al., Cloning characterization and ~unctional expression o~ cDNAs encoding glucose trans-porter proteins ~rom the human parasite Schistosoma mansoni,The Journal of Biological Chemistry 269:4247-53, 1994) are used. To target to vitelline glands, ~lanking sequences ~rom genes encoding eggshell proteins, such as pl4 (Kunz WK et al., Sequences o~ two genomic ~ragments containing identical coding region ~or a putative eggshell precursor protein o~
Schistosoma mansoni, Nucleic Acids Research 15:5894, 1987;
Koster B et al., Identi~ication of a putative eggshell precursor gene in the vitellarium o~ Schistosoma mansoni, Molecular and Biochemical Parasitology, 31:183-98, 1988) and p48 (Chen L, Rekosh DM, LoVerde PT, 1992) are used. Targeting expression to the Mehlis gland and ootype is likewise per-formed with genomic sequences ~rom Mehlis gland and ootype-speci~ic genes, such as the those coding ~or Mehlis gland and ootype secretory products, cloned by tissue-speci~ic differen-tial expression or subtractive hybridization approaches.
Figure 2B shows how analysis of the model gene promoter/enhancer is used to increase the ability o~ the transgene to compete with the endogenous gene for transcription factors, thus reducing expression of the model gene while increasing CA 02232~14 1998-03-19 expression o~ the transgene. This approach is applied to the eggshell model gene in particular, to reduce production o~
granuloma-provoking eggs. The construct is graphically identical to the one in Figure 2A, except for the addition o~
tissue-speci~ic core promoter elements (i). These sequences are identi~ied in the model gene (I) promoter/~nh~ncer region using standard DNAse protection and gel shi~t analyses and by sequence analysis for sequence moti~s conserved among promoters o~ di~erent eggshell genes as well as among di~erent species or strains. Then, a core element (i) (about 10 base pairs long), with its immediately-neighboring upstream and downstream sequences (about 80 base pairs long, in total) which likely contains binding sites ~or interacting transcrip-tion ~actors, is multimerized and reinserted into the promoter o~ the transgene expression construct (II) in its original location (e.g. in the proximal promoter) to increase tissue-speci~ic expression o~ the transgene. It is also placed near the ends o~ the DNA construct to sop up tissue-speci~ic transcription ~actors and reduce expression o~ the model (eggshell) gene.
The embodiment o~ this invention, depicted in Figure 2B
(a transgene expression construct with extra tissue-speci~ic core promoter elements), can be created without any prel; mi n~y experimentation. The vitelline gland eggshell gene p48 is the source of upstream and downstream promoter/
enhancer regions. sased upon their presence in several similarly regulated vitelline-speci~ic genes and upon evolutionary conservation to drosophila and silkmoth eggshell genes, several putative core promoter elements have already been identi~ied (Chen et al., 1992). One could use the 80 base pair region ~rom -335 to -255 as the repeated segment (i). This sequence contains two putative core elements. One o~ these, "TCAGCT" (-278 to -273) is also ~ound within the proximal promoters o~ the S. mansoni pl4 eggshell genes, as well as in silkmoth and drosophila eggshell genes (for a complete discussion, see Chen et al 1992). In drosophila, this element has been shown to be essential for pattern-speci~ic expression o~ the s36 chorion (eggshell) gene (Tolias PP, Konsolaki M, Hal~on MS, Stroumbakis ND, and Ka~atos FC, CA 02232~14 1998-03-19 WO 97/11191 PCT~US96/15083 Elements controlling ~ollicular expression o~ the s36 chorion gene during Drosophil a Oogenesis, Molecular and Cellular Biology 13:5898-5906, 1993).
B . Long-range Regul a tory Sequences Although flanking genomic sequences incorporated into the transgene vector are sometimes sufficient to target gene expression appropriately, "position ef~ectn can alter the expression pattern of a transgene. That is to say, depending upon the site of integration into genomic DNA, the transgene might be appropriately expressed, inappropriately expressed in undesired tissues, or not expressed at all. Usually, screening large numbers of recombinant organisms is sufficient to find a clone with the appropriate tissue-specific expression. Built-in higher order regulatory elements in the transgene vector can reduce the relevance of the site of transgene integration into the schistosome genome thus reducing the work to obtain the desired clones. Therefore, in one version of the transgene construct used in this invention, in addition to the nearby promoter and other flanking regulatory elements of the model gene, two types of distant cis-acting elements derived from the model gene are incorporated into the vector.
The ~irst type is a locus control region (LCR). These regulatory segments, first described within the alpha- and beta- globin loci, have now been discovered near several major gene clusters in vertebrates, including, for example, the recently described element near the T cell receptor alpha/delta locus (Diaz P, Cado D and Winoto A, A locus control region in the T cell receptor alpha/delta locus, Immunity 1:207-217, 1994). Presumably, through topological alteration of a block of DNA (i.e. unwinding), locus control regions confer tissue-specific activation of large regions of chromatin, facilitating regulation of neighboring genes expressed at similar times in a particular tissue. They are functionally defined as regions with a tissue-specific pattern of DNAse hypersensitivity that, when incorporated into an expression construct, confer a tissue-specific, copy-number-dependent, integration-site-independent level of transgene expression (Grosveld R, von Assendelft GB, Greaves DR, and . CA 02232~14 1998-03-19 W O 97/11191 PCTrUS96/15083 George Kollias, Position-independent, high-level expression of the human beta-globin gene in transgenic mice, Cell 51:975-85, 1987). The S. mansoni pl4 eggshell genes are clustered with two head-to-tail copies of one gene separated by a 7.5 kb region (Bobek LA, Rekosh DM, LoVerde PT, Small Gene Family encoding an eggshell (chorion) protein of the human parasite Schistosoma ma~soni, Molecular and Cellular Biology 1988 8:3008-16, 1988), localized to chromosome 2 (Harai H, Tanaka M
and LoVerde PT, Schistosoma mansoni: chromosomal localization of female-specific genes and a female-specific DNA element, Experimental Parasitology 76:175-81, 1993). It is likely that LCRs are important in their activation. LCR elements that regulate the model gene are identified based upon tissue-specific DNAse hypersensitivity pattern (Tuan D and London IM, Mapping of DNase I-hypersensitive sites in the upstream DNA of human embryonic epsilon-globin gene in K562 leukemia cells, Proceedings of the National Academy of Sciences (USA) 81:2718-22, 1984; Tuan D et al., The "beta-like-globin" gene domain in human erythroid cells, Proceedings of the National Academy of Sciences (USA) 82:6384-88, 1985). These are then incorporated into the transgene construct to further refine and support transgene expression.
Analysis of the genomic structure of the model gene is required to find long range cis-acting LCR sequences. The search for these sequences begins at the proximal promoter of the model gene and proceeds in both the 5~ and the 3' direction, to initially span up to lOOkb of DNA in either direction. The search is confined to the region of the genome expressed in the tissue of interest, so each DNA segment is first used as a probe of Northern blots of schistosome RNA
from the various tissues and life cycle stages. If, for example, a transcript that is expressed in the gut is identified 20 kb downstream of a model vitelline gland gene, then the LCR cannot lie beyond 20 kb downstream of the model gene. Thus, the region to be assayed for DNAse hypersensitive sites can be delimited. To find DNAse hypersensitive sites indicative of potential long range cis-acting LCR sequences, probes are generated near convenient restriction ~n~onllclease sites spaced every 2 to 5kb, and DNAse hypersensitivity assays CA 02232~14 1998-03-19 W O 97/11191 PCT~US96/15083 are per~ormed as described (Tuan et al PNAS, 1985, Tuan and London, PNAS, 1984). For each putative hypersensitive site, control chromatin ~rom a region o~ the worm not expressing the model gene or ~rom the opposite sex (~or ~emale-speci~ic transcripts) is used to determine i~ the hypersensitivity is tissue-speci~ic. Non-tissue-speci~ic DNAse hypersensitivity sites are putative boundary elements and serve to ~el ;m; t the region searched ~or long range cis-acting sequences. Figure 2C shows how a possible result o~ a search for an LCR is used to increase the activity o~ a transgene construct. In this hypothetical analysis, three upstream (j) and three downstream (k) tissue-speci~ic DNAse hypersensitive regions, each about lOObp, were identi~ied. These sites are subcloned into the original construct (Figure 2A).
Construction o~ the transgene expression vectors is assisted by the polymerase chain reaction using primers containing restriction sites to ~acilitate cloning, if convenient sites are not present in the genomic DNA. Plasmid subcloning o~ genomic sequences, genomic mapping and DNA
sequencing are per~ormed according to stAn~A~d techniques.
The other type o~ element to be incorporated into the vector is a boundary element. Boundary elements, recognized by their non-tissue-speci~ic pattern o~ DNAse hypersensitivity and by their ability to interact with speci~ic protein ~actors (Zhao K, Hart CM and Laemmli UK, Visualization o~ chromosomal domains with boundary element-associated ~actor BEAF-32, Cell 81:879-89, 1995), are sequences scattered throughout the genome which isolate genomic units, preventing an activated genomic region ~rom a~ecting neighboring transcription units (Kellum R and Schedl P, A position-e~ect assay ~or boundaries o~ higher order chromosomal ~o~A~nc~ Cell 64:941-50, 1991.
Boundary elements ~lanking the model gene are identi~ied as non-tissue-speci~ic DNA hypersensitivity regions or they are identi~ied on the basis of ability to ~unction as insulator elements in drosophila (Kellum and Schedl, 1991). Even copies o~ drosophila insulator/boundary elements (Udvardy A, Maine E, and Schedly P, The 87A7 chromomere: Identi~ication o~ novel chromatin structures ~lanking the heat shock locus that may de~ine the boundaries o~ higher order domains, Journal o~

, CA 02232~14 1998-03-l9 WO 97/11191 PCTrUS96/150~3 Molecular Biology 185:341-358, 1985; Farkas G and Udvardy A
Sequence of scs and scs' Drosophila DNA fragments with boundary function in the control of gene expression, Nucleic Acids Research 20:2604, 1992, incorporated herein by reference) could be used for this purpose, a5 the sequences display high evolutionary conservation, being functional in distantly related species (Chung JH, Whiteley M and Felsenfeld G, A 5' element of the chicken beta-globin domain serves as an insulator in human erythroid cells and protects against position effect in Drosophila, Cell 74:505-14 et al., 1993).
Therefore, one would expect the drosophila sequences to also function in schistosomes.
In one version of the construct used in this invention (Figure 2D), boundary elements (l)(from drosophila or identified during the search for LCR sequences) are added to the ends of the transgene vector depicted in Figure 2C outside of the LCR. This step is to promote regulated transcriptional control by preventing inappropriate activation of schistosome genes near the site of transgene integration and by functionally isolatin~ the transgene from its surroundlng host chromatin.
Thus, with the boundary elements, LCR, and the pro~; m~ 1 promoter and flanking elements incorporated into the vector, all of the sequences sufficient to confer integration-site-independent, copy-number-dependent and tissue-specific expression of the transgene are utilized Not all of these elements are incorporated into each version of the vector, and simple vectors lacking distant LCR and boundary elements may prove to function adequately when numerous recombinants can be obtained. The identification of LCRs should not be considered undue experimentation, although it may involve considerable work, because the techniques involved (genomic DNA subcloning and mapping, Northern blotting and DNAse hypersensitivity assays) are routine. Isolation of these regions is simply a - 35 matter of iteration. The ultimate determinant of vector adequacy rests upon assay of protein expression by transgenic ~ adult worms.
C. Additional considerations ~or vector design CA 02232~14 1998-03-19 W O 97/11191 PCT~US96/15083 The transgene vector construct can accommodate several kilobases of coding sequence with about 7 kb o~ ~lanking regulatory DNA. The length of the regulatory region o~ the transgene vector is ~ln;m;zed in order to save room for transgene coding regions and in order to m~; m; ze the number of molecules of DNA that can be injected into the egg, to increase the probability o~ recombination. Biochemical and functional assays are per~ormed to obtain the smallest sufficient regulatory regions, ret~;n;ng only m;n;m~lly suf~icient LCR and promoter elements. In biochemical assays the functional sequences of the regulatory cis-acting regions are highlighted by tissue-specific DNAse hypersensitivity patterns. With sequential deletions of the initial construct, the minimally su~icient regions are ~urther defined in vivo by testing their ability to regulate a reporter gene. For example, the cDNA ~or betagalactosidase could be incorporated into the vector, and the e~ectiveness o~ the regulatory sequences assessed by staining the adult worms with X-gal.
In addition, nuclease hypersensitivity studies require relatively pure cell preparations in order to identify the LCR
and boundary elements. Vitelline gland cells are the predominant cell type in posterior female worm segments and in early eggs. Dissected tissue from these regions provides convenient source material for characterization o~ the eggshell genes. Identification o~ this region is ~acilitated by in situ fluorescent histochemistry (Bennet JL, Seed JL, Boff M, Fluorescent histochemical localization of phenol oxidase in ~emale S. mansoni, Journal of Parasitology 64:941-44, 1978).
IV. Isol a ti on of Model Genes Genomic sequences corresponding to tissue-speci~ic genes o~ interest are obtained ~rom a schistosome genomic DNA
library (lambda phage, cosmid or phage Pl) using standard DNA
hybridization techniques. Hybridization probes include cDNA
and genomic DNA obtained ~rom cooperating investigators, generated via PCR using oligonucleotides based on published sequences and obtained from schistosome libraries. Some of CA 02232~14 1998-03-l9 the relevant cDNAs are identified by subtractive cDNA
hybridization.
Identification of Mehlis gland-, ootype-, and vitelline cell- specific gene products by construction of subtracted cDNA libraries. The sequences of several vitelline gland-specific cDNAs such as those encoding the pl4 and p48 eggshell proteins are known and can be used as probes of genomic libraries to find the relevant regulatory sequences.
However, in some embodiments of this invention the transgene is expressed in the Mehlis gland and the ootype, sites which have yet to be assigned tissue-specific genes. It is not necessary to know the function of these gene products per se, since only the regulatory regions of these genes are used. A
straightforward approach to ~inding these genes is subtractive cDNA hybridization (Palazzolo MH et al., Use of a new strategy to isolate and characterize 436 drosophila cDNA clones corresponding to RNAs detected in adult heads but not embryos.
Neuron 3:527-39, 1989; Sive HL, St John T, A simple subtractive technique employing photoactivatable biotin and phenol extraction. Nuceic Acids Research 22:10937, 1988). In other embodiments of this invention, it is necessary to identify vitelline gland-specific transcription factors and tannase and phenolase enzymes. Here too, subtractive cDNA
hybridization can be used to identify candidate genes that are further subjected to sequence analysis, with putative identification based upon sequence homology to known genes.
This process entails a considerable amount of work that is iterative in nature, but it has been performed successfully in many laboratories and does not require undue experimentation.
Subtractive cloning can be applied to find model genes.
Whole female worm cDNA or cDNA from dissected female vitelline gland or Mehlis gland/ootype regions are depleted of male-expressed sequences by hybridization of female cDNA with photobio-tinylated driver male RNA. To avoid isolation of known and abundant female cDNA clones, in vitro transcribed known female-specific genes (e.g. pl4 and p48 mRNA) are added to the driver population. A cDNA library is generated from this female-specific cDNA population. To characterize the spatial and temporal expression patterns of individual clones CA 02232~14 1998-03-l9 W O 97/11191 PCTrUS96/15083 in this library, Northern blot hybridization is used initially. Female worms are sectioned into anterior (Mehlis gland and ootype) region and posterior (vitelline gland) region, guided by in situ fluorescent histochemical identification of the vitelline gland region (Bennet et al, 1978). Also, mRNA from immature female worms from unisexual female infections and from eggs is isolated. The subtracted cDNAs are sorted by cross-hybridization pattern to identify clones of the same gene and by Northern blot hybridization to the various schistosome mRNA preparations mentioned. Probes from clones abundantly and specifically expressed in the vitelline gland, Mehlis gland and ootype regions are used for in si tu hybridization to confirm the cell type of expression.

V. Interferinq wi th Schistosome Females ' Eqqshell Production For female worm clones with transgene expression targeted to the Mehlis gland, ootype or vitelline cells, interference with egg shell protein production or eggshell maturation is desirable to prevent provocation of a granulomatous response to viable eggs. Such shell-de~icient eggs could be broken down by the host without provoking granuloma formation, as evidenced by the ef~ect of vitamin C deficiency, which prevents eggshell hardening and granuloma formation in infected ~n~m~ls (Krakower C, Hoffman WA, Axtmayer JH, Defective granular eggshell ~ormation by Schisto50ma mansoni in experimentally infected guinea pigs on a vitamin C
deficient diet, Journal of Infectious Diseases 74:178-83, 1944). In general, in strategies targeting expression to female glands, two types o~ genetic modifications of the schistosome are required: the first one interferes with eggshell production, the second introduces the transgene.
This sequential task is accomplished by performing the first modification in male worms (which become asymptomatic carriers) and the second one in their female, affected progeny.
Figure 3 shows a flowchart of the technique for creating transgenic schistosomes harboring a knockout construct for an eggshell gene or eggshell maturation enzyme. Female worms, paired with males in infected mouse intestines (A), lay stage ~ CA 02232~14 1998-03-l9 WO 97/11191 PCT~US96/1~083 I eggs (B) in vivo or in vitro. As described above with regard to Figure 1, the stage I eggs are identified by a central ~ertilized egg that contains male and female pronuclei (i), surrounded by vitelline cells (ii) and a thin eggshell (iii). Both male and female stage I eggs are microinjected with a knockout DNA construct because they cannot be distinguished at the time of microinjection. This construct is designed to knock out an eggshell gene, an eggshell maturation enzyme (tyrosine hydroxylase or phenolase (see below)) or a vitelline specific transcription factor (see below). Karyotyping is performed on the clones at the sporocyst stage in infected snails (C), and female clones are discarded. Male cercaria (D) harboring the knockout construct are used to infect mice (E) in co-infections with wild type female cercaria. Normal female and male eggs harboring the eggshell knockout construct (F) are laid, because the vitelline cells are contributed by the wild type female parent. Both male and female eggs are now microinjected with the transgene construct, but this time ~emale sporocyst clones (G) that have inherited the knockout construct and incorporated the transgene construct are retained. The cercaria (H) derived ~rom these sporocysts are used with normal male cercaria to co-infect patients, producing viable adult worm pairs that lay so~t eggs and produce large amounts o~ transgene product. Alternatively, transgenic ~emale lines are obtained on a wild type strain background. If they are fertile, then they can simply be crossed with the male knockout construct-carrying line (D) by co-infection of mice, with selection of progeny female sporocyst clones harboring both the transgene and the knockout constructs.
RNA Knockout Vectors can be speci~ically designed.
Antisense RNA is used naturally in diverse organisms to mediate destruction o~ complementary RNA strands, presumably by annealing with it and activating its digestion by RNAses~ 35 (reviewed by Delihas N, Regulation o~ gene expression by trans-encoded antisense RNAs, Molecular Microbiology 15:411-14, 1995). Hundreds of experiments have utilized this concept to down regulate specific messages, and the technique has proven highly use~ul for reducing biological activity o~

CA 02232~14 1998-03-19 W O97/11191 PCTfUS96/15083 dozens o~ transcription ~ac~ors in cell lines (e.g. Reis LF et al., Critical role of a common transcription factor, IRF-1, in the regulation of IFN-beta and IFN-inducible genes, E~30 Journal 11:185-93, 1992) and in transgenic ~n~m~ (e.g.
Matsumoto K et al., Evaluation of an antisense RNA transgene for inhibiting growth hormone gene expression in transgenic rats, Developmental Genetics, 16:273-77, 1995) and plants (e.g. Kuipers AG et al., Factors a~fecting the inhibition by antisense RNA of granule-bound starch synthase gene expression in potato, Molecular and General Genetics 246:745-55, 1995).
Antisense constructs are generated by incorporating the cDNAs to targeted transcription ~actor, t~nn~ng enzyme or eggshell protein genes in reverse orientation into the expression construct, as described below.
Ribozyme constructs have also been shown to have biological utility, although it is unclear whether in practice, they are more effective than antisense RNA (James W
and Al-Shamkhani A, RNA enzymes as tools for gene ablation, Current Opinion in Biotechnology, 1995 6:44-49, 1995).
Ribozyme expression constructs have been used in transgenic ~nim~ls to knockout targeted gene function (Zhao JJ and Pick ~, Generating loss-o~-function phenotypes of the fushi tarazu gene with a targeted ribozyme in Drosophila, Nature 365:448-51, 1993). Ribozyme constructs are created with the h~mme~head ribozyme incorporated into short regions homologous to targeted schistosome mRNA se~uence, akin to antisense RNA
constructs.
Five strategies are described below for inter~ering with eggshell production: (1) Express the transgene at sufficiently high levels such that it reduces eggshell expression by competing for factors essential for transcription, translation and protein processing of the eggshell gene products; (2) Use speci~ic RNA-mediated knockout constructs (ribozyme or antisense) to destroy vitelline eggshell mRNAs; (3) Use speci~ic RNA-mediated knockout constructs to interfere with essential steps in eggshell maturation; (4) Interfere with transcription of dif~erentiated vitelline cell-speci~ic genes by RNA-mediated knockout of an essential vitelline cell transcription factor; and (5) Interfere with transcription of CA 02232~l4 l998-03-l9 WO 97/11191 PCTrUS96/15083 differentiated vitelline cell-specific genes by expression of a dominant-negative or competitive inhibitor of an essential vitelline cell transcription factor.
In the first strategy, high level expression of the transgene in the vitelline cell directed by regulatory sequences of an eggshell gene may reduce t~nn~hle eggshell protein below levels needed to make functional t~nn~hle eggshells. Instead of releasing viable eggs, the gravid female releases the fertilized ova in a sea of transgene product. This high level expression is accomplished by the use of the most developed form of the vector, including boundary domain elements to flank the construct, and incorporation of relevant LCR-like and local enhancer and promoter elements to obtain position-independent, copy-number dependent expression, as outlined above in Figure 2D. The endogenous eggshell genes are present in the genome in multiple copies but they are not amplified as are the chorion genes of drosophila. The pl4 gene may only be present at about three copies per haploid genome of S. mansoni (Bobek et al, 1988 (see above)), and p48 is probably only present in a single copy, based on its low frequency in an unamplified genomic library (1 per 140,000 for p48 versus 6/100,000 for pl4, Bobek, 1988, Chen, 1992 (see above)). At least two of the pl4 genes are contiguous, separated by 7.5 kb of intergenic DNA, and arranged tail to head. Thus, if each transgene construct copy is transcribed at the same level as an endogenous pl4 or p48 eggshell gene, then it would require 80 copies of the transgene construct to reduce eggshell protein production by 90~. This copy number is attainable by cross-breeding independent transgene integrants, considering that low transgene copy number clones are likely to remain fertile, because ipso ~acto they have preserved vitelline ~unction. If an average transgenic schistosome carries five t~n~e~ copies of the transgene, then four generations of~ 35 cross-breeding three independent clones and selection of progeny achieves this goal.
Using the second strategy, RNA-mediated "knockout"
constructs of eggshell genes are unlikely to significantly interfere with expression of these genes in the wild type CA 02232~14 1998-03-19 W O 97/11191 PCTrUS96/15083 schistosome, since the target genes are expressed at high levels. However, expression of such knockout constructs may act synergistically with the above strategy to reduce already low egyshell gene transcripts to even lower levels.
The third strategy for inter~ering with eggshell production involves targeted inter~erence with eggshell maturation. The rationale for this is that the schistosome egg shell requires enzymatic tanning (cross-linking) in order to harden. This outer shell is produced ~rom secretory products o~ the vitelline cells which envelop the egg in the worm uterus. Some o~ these cells adhere to the ~ertilized o w m and line the egg underneath the eggshell (LoVerde PT, and Chen L, Schistosome Female Reproductive Development, Parasitology Today 7:303-08, 1991). The main component of the eggshell is sclerotin, a proteinaceous material that has undergone a quinone-dependent tanning process (Nollen PM, Digenetic trematodes: Quinone tanning system in eggshells, Experimental Parasitology, 30:64-67,1971; Wharton DA, The production and functional morphology o~ hel m; nth eggs shells, Parasitology, 86 Suppl:85-97,1983; Smyth JD and Halton DW, The physiology o~ Trematodes, 2nd Ed. Cambridge, Cambridge University Press, 1983) which cross-links and hardens the initially soft eggshell proteins. There is chemical evidence that the pathway involves phenols (i.e. dihydroxyphenylala-nine) which are derived ~rom modi~ication of the numeroustyrosine residues that are present in the characterized eggshell proteins (Chen et al, 1992). These proteins are produced by the vitelline cells and released in vitellire granules along with an enzyme ~phenolase" (Seed JL, Bo~f M and Bennett JL, Phenol oxidase activity: Induction in ~emale schistosomes by in vitro incubation, Journal of Parasitology 64:283-89, 1978, which oxidizes these residues to the highly reactive quinone. The quinones are thought to cross-link to exposed lysyl residues that are also present abundantly in the eggshell protein molecules (Chen L. et al, 1992), hardening the shell o~ the schistosome egg.
The result o~ inter~ering with the eggshell tanning process is to prevent sclerotin hardening. The residual non-polymerized eggshell protein is not expected to provoke CA 02232~14 1998-03-19 disease, as mice treated with inhibitors of egg tanning do not develop hepatosplenomegaly (Bennett JL and Gianutsos G, ~ Disulfuram: a compound that selectively induces abnormal egg production and lowers norepinephrine leveles in S. Mansoni, Biochemical Pharmacology 27:817-20, 1978). An additional benefit is that for transgene products secreted by vitelline cells, softening the eggshell can be expected to facilitate diffusion of the transgene product into the host bloodstream instead o~ potentially becoming entrapped in the eggshell matrix.
Cloning eggshell maturation genes: The schistosome enzymes involved in this process have not yet been isolated.
They are a putative tyrosine hydroxylase and a phenol oxidase.
The tyrosine hydroxylase gene is to be cloned based on the extensive evolutionary sequence conservation. For example, the tyrosine hydroxylase genes of the fruit fly Drosophila melanogaster (Nechameyer WS and Quinn WG, Neuron 2:1167-75, 1989) and of the cow (Saadat S et al., J. Neurochemistry, 51:572-78, 1988) are 76~ identical between drosophila amino acid number 291 and 451. They are 71~ identical in this region at the nucleotide level. Thus, the drosophila sequence is used to probe at low stringency cDNA libraries made ~rom ~emale schistosomes or from early stage eggs, sources o~
vitelline mRNA. Alternatively, degenerate oligonucleotide primers based on the most conserved regions are used to generate an RT-PCR product for subsequent high stringency cDNA
library screening (see Appendix).
A phenolase from Drosophila melanogaster involved in sclerotinization of the exoskeleton has been cloned by genetic means and its sequence has been published (Pentz ES and Wright TRF, Drosophila melanogaster diphenol oxidase A2: gene struc-ture and homology with the mast-cell tum(-) transplantation antigen, P9lA Gene 103:239-42, 1991). Thus, low stringency screening o~ a female schistosome cDNA library may identify the clone of interest. In another strategy, expression cloning is used, with selection based upon enzymatic activity.
~ A bacteriophage cDNA expression library is constructed (e.g.
in Stratagene lambda Zap II). Induction of protein expression by IPTG is per~ormed in the presence of O.OlM catechol and CA 02232~14 1998-03-19 WO 97/11191 PCT~US96/15083 assayed either on the agarose plates or on nitrocellulose filter lifts. The phenolase positive clones are identified by fluorescence at 537 nm when excited in the ultraviolet range (Bennet et al., 1978) or by the red color of the oxidation product (Wang FL et al., Isoenzymes of phenol oxidase in adult female Schistosoma japonicum, Molecular and Biochemical Parasitology 18:69-72, 1986). Alternatively, the phenolase and tyrosine hydroxylase are identified by subtractive hybridization, in which case schistosome tyrosine hydroxylase is recognized based upon predicted homology to other members of the family, and phenolase is recognized based upon homology to known oxidases. The cDNAs encoding both of these enzymes must show high level expression in the vitelline cells.
Tyrosine hydroxylase is likely to be expressed in neurons as well.
A third potential target in this pathway is an activator of tyrosine hydroxylase, which has been found in m~mm~l S as well as drosophila, and has been cloned (Swanson KD and Ganguly R, Characterization o~ a Drosophila melanogaster gene similar to the mammalian genes encoding the tyrosine/trypto-phan hydroxylase activator and protein, kinase C inhibitor proteins, Gene 113:183-90, 1992). It is not presently known if a similar activator is re~uired for schistosome tyrosine hydroxylase activity. In vivo interference with the mRNAs encoding eggshell maturation enzymes is to be performed via antisense RNA or ribozyme constructs.
The ~ourth strategy for inter~ering with eggshell production involves blocking vitelline cell transcription factors. The most efficient way to reprogram vitelline cells to make the transgene product in place o~ eggshell proteins would be by manipulating expre~sion o~ dif~erentiated vitelline cell-specific transcription ~actors that coordinate expression o~ the gene products of differentiated vitelline cells. Typical transcription ~actors are expressed at levels orders of magnitude below many other gene products. In vi tro and in vivo RNA-mediated knockout are most successful when the targeted moiety is expressed at low levels. (Cameron and Jennings, Antisense Res and Dev, 4:887-94, 1994) There~ore, RNA-mediated knockout strategies are expected to be more _ CA 02232~14 1998-03-19 successful when applied to these regulatory genes than when directed towards the gene products the~ regulate.
Vitelline gland maturation proceeds through four stages, with vitelline droplets appearing in the last stage (Erasmus DA, schistosoma mansoni: development of the vitelline cell, its role in drug sequestration and changes induced by Astiban, Experimental Parasitology 38:240-56, 1975). The orderly pattern of cellular differentiation suggests the existence of stage-specific transcription factors to coordinate gene expression, with eggshell and tanning mRNAs expressed as a result of activation of the transcription factors of the terminally differentiated cell. The homologous promoter elements within the silkmoth and drosophila chorion genes and the Schistosoma mansoni eggshell genes (Chen et al., 1992~ are a starting point to clone candidate vitelline-specific, late-acting transcription factors. Cloning the factors that bind to those elements is readily achievable. Two methods have been routinely employed in this regard to clone dozens of DNA binding factors: (1) purification of the binding factor based upon affinity to synthetic oligonucleotides bearing the target sequence, followed by amino acid microsequencing and design of degenerate probes or PCR primers based upon reverse translation, and (2) direct probing of expression libraries with target element oligonucleotides. In addition, transcription factors can be identified on the basis of sequence homology after finding candidate tissue-specific genes by subtractive cDNA hybridization. This last method requires no prior information regarding specific target elements. After identification, these vitelline cell-specific transcription factors are to be targeted for interference by ribozyme and antisense constructs.
The fifth eggshell production interference strategy is dominant-negative interference with transcription factor activity. Current understanding of functional domains of many transcription factors as predicted their by primary amino acid sequence suggests a way to interfere with their function at the protein level. In general, the transcriptional activation domain is identifiable as a negatively charged amphipathic helix or a proline and/or serine/threonine rich domain. In CA 02232~14 1998-03-19 several cases, expression of a truncated version that lacks the essential transcriptional activation domain has been shown to inhibit the ~unction o~ the endogenous wild-type ~actor by competition for target DNA elements (Langer SJ et al., Mitogenic signaling by colony-stimulating factor 1 and ras is suppressed by the ets-2 DNA-binding domain and restored by ~yc overexpression, Molecular and Cellular Biology 12:5355-62, 1992; Lloyd A, Yancheva N and Wasylyk B, Trans~ormation suppressor activity o~ a Jun transcription ~actor lacking its activation domain, Nature, 352:635-38, 1991; Smith VM et al., GATA-3 dominant negative mutant, Functional re~lln~n~y of the T cell receptor alpha and beta ~nh~ncers, Journal o~ Biolo-gical Chemistry, 270:1515-20, 1995). Also, ~or transcription factors that bind to DNA as homodimers, overexpression o~ a truncated ~orm of the transcription ~actor lacking the DNA-binding portion o~ the protein has been shown to inhibit the function of the wild-type molecule in a dominant-negative ~ashion (Logeat F et al., Inhibition o~ transcription factors belonging to the rel/NF-kappa B ~amily by a transdom;n~nt negative mutant, EMBO Journal 10:1827-32, 1991; Beckman H and Kadesch T, The leucine zipper o~ TFE3 dictates helix-loop-helix dimerization speci~icity, Genes and Development 5:1057-66, 1991), similar to the naturally ~ound Id ~amily (Benezra R et al., The protein Id: a negative regulator o~
helix-loop-helix DNA binding proteins, Cell 61:49-59, 1990) and to CHOP (Ron D, Habener JF, CHOP, a novel developmentally regulated nuclear protein that dimerizes with transcription ~actors C/EBP and LAP and functions as a dominant-negative inhibitor o~ gene transcription, Genes and Development 6:439-53, 1992) ~actors. Most remarkably, ~or dimeric transcription factors cont~;n;ng leucine zipper moti~s, simple rules have been derived based upon charge interaction to design ef~ective competitive inhibitors (Vinson CR, Hai T and Boyd SM, Dimerization specificity of the leucine zipper-cont~;n;ng bZIP moti~ on DNA binding: prediction and rationaldesign, Genes and Development 7:1047-58).
It should be noted that knockout of a vitelline cell transcription ~actor complicates the strategy ~or transgene expression in strategies 4 and 5 above. To drive expression CA 02232~14 1998-03-19 WO 97/11191 PCTrUS96/15083 in the transcriptionally crippled vitelline cells, the regulatory regions of the knockout construct are derived from a highly expressed, ubiquitous housekeeping gene. The promoter of such a gene does not require a vitelline cell-specific transcription factor for activity. Such a model genecould be one encoding a ubiquitous ion transporter (e.g. Na/K
ATPase) or a glycolytic enzyme (e.g. triosephosphate isomerase (dos Reis MG et al., Characterization of the Schistosoma mansoni gene encoding the glycolytic enzyme triosephosephate isomerase, Molecular and Biochemical Parasitology 59:235-42, 1993)). The antisense RNA, ribozyme, or dominant negative knockout factor is active only in vitelline cells, where its target is present. The resulting vitelline cells, however, cannot be used to express the transgene under control of eggshell gene promoter elements. Thus, strategies 4 and 5 are feasible in conjunction with transgene expression in other tissues (e.g. the Mehlis gland and the ootype), where the vitelline gland specific transcription factor is irrelevant.
This is illustrated in Figure 4A as a two-step strategy of schistosome genomic modification. In the first step (1) (see Figure 3, step B), eggs are microinjected with DNA from a plasmid construct containing a vitelline transcription factor knockout transcript (a) (i.e. ribozyme, antisense RNA, or dominant negative transcription ~actor). This transcript is under control of promoter/enhancer and LCR regions (b and b') taken from a housekeeping gene. In the second step (2) (see Figure 3, step F), a transgene whose expression is targeted to the Mehlis gland or ootype is microinjected, using DNA from a plasmid construct containing the transgene (c), flanked by regulatory promoter/enhancer and LCR se~uences (d and d') taken from a Mehlis gland or ootype-specific model gene.

VI. Reprogramming T~r~m;n~7 Vitelline Cell Gene Expression ~y Introduction of a Re~lacement transcriPtiOn Factor.

To take advantage of vitelline cell transgene targeting in conjunction with strategies to decrease eggshell gene transcription through interference with their specific transcription factors (strategies 4 and 5 above), a surrogate factor is designed to activate the transgene, and eggshell CA 02232~14 1998-03-19 protein expression is usurped by transgene expression. This is illustrated as a three step strategy of schistosome genomic modification in Figure 4B. In the first step (1) (see Figure 3, Step B), schistosome eggs are microinjected with DNA ~rom a knockout construct encoding a transcript (a) targeting a vitelline gland tissue-specific transcription factor (TFA).
The knockout constructs designed to antagonize TFA could be activated by non-tissue-speci~ic "housekeeping gene"
r~g~l~ory se~uences (b and b' in construct la) or by tissue-specific regulatory sequences (constructs lb and lc).
In the ~ormer case, the TFA knockout transcript (a) would be expressed in all cell types due to "housekeeping gene"
promoter/enhancer sequences (b and b'), but it would be active only in mature vitelline cells, where it would encounter and inactivate TFA. In the latter case, tissue-specific expression of the TFA knockout RNA (a) could be achieved by utilizing the promoter context of TFA itsel~ (c and c' in construct lb), which would assure synchronous expression, but not high levels o~ expression. Alternatively (lc), tissue-specific expression of the TFA knockout RNA (a) in the context o~ regulatory sequences ~rom a downstream eggshell gene (d and d~) would enable high level expression of the knockout construct, but it would also allow transient expression o~ the eggshell genes.
In this three step strategy (Figure 4B), vitelline cell-speci~ic expression o~ the transcript ~or the therapeutic transgene (h in construct 3) could not be facilitated simply by insertion within the context o~ eggshell gene regulatory sequences, as this would be inactive due to 1088 o~ TFA.
Also, in the absence o~ TFA, the tissue-specific TFA knockout construct would cease to be transcribed (lb and lc). To overcome this problem, the following scheme is employed: the cDNA for a transcript (e) encoding a replacement transcription factor B (TFB) is inserted into a construct in the second step (2) utilizing the vitelline cell-speci~ic regulatory sequences o~ either TFA itsel~ (c and c~ in construct 2a) or o~ an eggshell gene (d and d' in construct 2b).
TFB is a chimeric protein consisting of TFA with its DNA-binding domain replaced by a DNA-binding domain with a CA 02232~14 1998-03-19 W O 97/11191 PCTrUS96/15083 different and known specificity, choosing a replacement DNA-binding domain of the same class (e.g. basic, zinc finger, helix-turn-helix, homeobox.) However, if a ~o~;nAnt-negative knockout is used, only the transcriptional activation ~o~; n of TFA is incorporated into TFB, to avoid heterodimerization with the dominant-negative protein. As shown in the shaded inset, in the transgene expression vector (construct 3), all binding sites (e) for TFA within endogenous eggshell gene regulatory regions (exemplified by (f)) are functionally replaced by adding adjacent TFB binding sites (g). TFA sites within the regulatory region o~ the TFA knockout construct (lb and lc) and of the TFB expression construct (2a, 2b) are likewise replaced by TFB binding sites, as indicated by the grey and the black boxes in elements (c) and (d) in Figure 4B.
This step facilitates positive feedback regulation of the surrogate transcription factor TFB to mimic a likely aspect of TFA biology and stabilizes the TFA knockout expression. (This three step strategy is theoretically feasible for knockout of any terminal vitelline cell-specific transcription ~actor, barring the possibility that two or more necessary factors are both coordinately expressed and stabilize one another's expression. In that case it would be necessary to also replace the other factors, which could be done in the same expression construct as for TFB.) This re-engineering of the vitelline cell gene regulation requires expression o~ two constructs (the TFA knockout construct and the TFB expression construct) in a male worm line to be transmitted as a do~;n~nt trait to progeny eggs to be used for subsequent microinjection of the therapeutic transgene. Co-injection of the TFA knockout and TFB
expression constructs (in step B in Figure 3) is the simplest approach to obtaining this desired intermediate male clone.
However, the TFB construct should be biologically silent even in females, so the triply transgenic lines can also be obtained by stepwise injection of the constructs into eggs of successive generations. This allows an intermediate step of crossing of TFB-carrying lines to titrate for optimal copy number. To wit, the TFB expression construct can be injected at step F ~see Figure 3). Instead of discarding male CA 02232~14 1998-03-19 sporocyst clones at step G, the double transgenic males (harboring both the TFA knockout and the TFB expression construct) are mated with normal females by co-infection of mice (step E, Figure 3). In the final step, the transgene construct is injected into the ensuing eggs, and female clones harboring all three constructs are selected ~or use.
It will be further apparent to one skilled in this art that the improvements provided for in the present invention, while described with relation to certain specific physical embodiments also lend themselves to being applied in other physical arrangements not specifically provided for herein, which are nonetheless within the spirit and scope of the invention taught here.

CA 02232~14 1998-03-19 W O 97/11191 PCTrUS96/15083 APPENDIX: PCR-CLONING OF SCHISTOSOME TYROSINE 3 HYDROXYLASE
GENE
Oligonuceotides ~or PCR of tyrosine 3-hydroxylase gene ~rom vitelline cell cDNA:
Upstream primers (based on drosophila TY3H amino acids 368-373) 5'GA(T/C)GA(G/A)GA(G/A)ATTGA(G/A)AA(A/G)(T/C)T3' 5'GA(T/C)GA(G/A)GA(G/A)ATAGA(G/A)AA(A/G)(T/C)T3' 5'GA(T/C)GA(G/A)GA(G/A)ATCGA(G/A)AA(A/G)(T/C)T3' Downstream primers (based on drosophila TY3H amino acids 428-433) 5'TG(A/G)TC(T/C)TG(G/A)TANGG(C/T)TGTAC3' 5'TG(A/G)TC(T/C)TG(G/A)TANGG(C/T)TGCAC3' 5'TG(A/G)TC(T/C)TG(G/A)TANGG(C/T)TGAAC3' 5'TG(A/G)TC(T/C)TG(G/A)TANGG(C/T)TGGAC3' When combinations o~ upstream and downstream primers are used in PCR reactions at annealing temperature o~ 50-60~C, a 195 bp product is expected ~rom vitelline cell RNA ~ources.

Claims (37)

1. A method for producing transgenic schistosomes for secretion of desired gene products comprising:
a. identifying a gene whose expression is desired;
b. subcloning a protein-coding portion of a cDNA from said gene into a plasmid containing local upstream and downstream promoter/enhancer sequences, and containing 5' and 3' untranslated regions of a schistosome gene expressed in the integument, vitelline or Mehlis glands, or ootype of schistosomes;
c. microinjecting the transgene DNA into the pronuclei or cytoplasm of the zygotes of stage I schistosome eggs;
d. culturing said schistosome eggs to maturity in vitro;
e. allowing miracidia to hatch;
f. infecting snails on a one-miracidium-per-snail basis;
g. culturing said snails until cercaria of schistosome clones are produced;
h. identifying transgenic schistosome clones at the cercarial stage;
i. maintaining said transgenic schistosome clones by sporocyst transfer;
j. infecting an animal host with said cercaria of said transgenic schistosome clones on a one-clone-per-animal basis;
and k. identifying transgenic schistosome clones secreting high levels of transgene product into peripheral blood of said animal host.

2. The method of claim 1 wherein step (c) further comprises microinjecting the transgene DNA into the pronuclei or cytoplasm of the zygotes of stage I schistosome eggs laid in vitro.

3. The method of claim 1 wherein step (c) further comprises microinjecting the transgene DNA into the pronuclei or cytoplasm of the zygotes of stage I schistosome eggs dissected from tissues of an infected animal.

4. The method of claim 1 further comprising after step (h) the additional step of determining the gender of said transgenic schistosome clones at the cercarial stage, and wherein step (j) further comprises infecting an animal host with only male cercaria of said transgenic schistosome clones.

5. The method of claim l further comprising after step (h) the additional step of determining the gender of said transgenic schistosome clones at the cercarial stage, and wherein step (j) further comprises co-infecting an animal host with wild type male cercaria and with said cercaria of female transgenic clones on a one transgenic-clone-per-animal-basis.

6. The method of claim 1 wherein step (b) further comprises subcloning cDNA encoding schistosome protein secretory targeting sequences in the 5' region of the coding portion of the transgene into said plasmid.

7. The method of claim 1 wherein step (b) further comprises identifying putative functional areas of said promoter/enhancer regions by interspecies or interstrain sequence conservation or DNAse protection or gel retardation assays, and deleting from said plasmid segments between functional areas.

8. The method of claim 1 wherein step (b) further comprises creating plasmid subclones with progressive unidirectional or nested deletions of said promoter/enhancer regions of said plasmid.

9. The method of claim 1 wherein step (b) further comprises subcloning additional binding sites for tissue-specific transcription factors into said promoter/enhancer regions of said plasmid.

10. The method of claim 1 wherein step (b) further comprises subcloning locus control region elements flanking said promoter/enhancer regions of said plasmid.

11. The method of claim 1 wherein step (b) further comprises subcloning boundary domain elements flanking the promoter/enhancer regions of said plasmid.

12. The method of claim 1 wherein said stage I
schistosome eggs contain zygotes harboring eggshell knockout constructs produced by the steps of:

l. identifying a target gene, being a schistosome eggshell gene, the gene encoding a putative transcription factor that up-regulates a schistosome eggshell gene, or the gene encoding an enzyme required for maturation of the schistosome eggshell;
m. identifying a ubiquitously expressed schistosome gene;
n. creating an eggshell knockout construct by subcloning an eggshell knockout cDNA into a plasmid containing local upstream and downstream promoter/enhancer sequences and 5' and 3' untranslated sequences derived from said ubiquitously expressed schistosome gene, from said schistosome eggshell gene or from said eggshell gene transcription factor;
o. microinjecting said knockout construct into the pronuclei or cytoplasm of the zygotes of stage I schistosome eggs;
p. culturing said schistosome eggs to maturity in vitro;
q. allowing miracidia to hatch;
r. infecting snails on a one-miracidium-per-snail basis;
s. culturing said snails until cercaria of schistosome clones are produced;
t. identifying at the cercarial stage clones carrying said knockout-construct;
u. determining the gender of said schistosome clones carrying said knockout construct;
v. maintaining said knockout-construct-carrying-clones by sporocyst transfer;
w. co-infecting an animal host with said cercaria of said knockout-construct-carrying-clones; and x. harvesting stage I eggs of said knockout-construct-carrying-clones.

13. The method of claim 12 further comprising in step (n) identifying putative functional areas of said promoter/enhancer regions by interspecies or interstrain sequence conservation or DNAse protection or gel retardation assays, and deleting from said plasmid segments between said functional areas.

14. The method of claim 12 wherein step (n) further comprises creating plasmid subclones with progressive unidirectional or nested deletions of said promoter/enhancer regions of said plasmid.

15. The method of claim 12 wherein step (n) further comprises subcloning locus control region elements flanking said promoter/enhancer regions of said plasmid.

16. The method of claim 12 wherein step (n) further comprises subcloning boundary domain elements flanking said promoter/enhancer regions of said plasmid.

17. The method of claim 12 wherein step (n) further comprises creating said eggshell knockout construct by cloning cDNA sequences from said target gene into said plasmid in inverted orientation.

18. The method of claim 17 wherein step (n) further comprises cloning said inverted target gene cDNA sequences into said plasmid in the context of hammerhead ribozyme sequences.

19. The method of claim 12 wherein step (n) further comprises subcloning the cDNA encoding the putative DNA-binding domain but not the trans-activating domain of the said eggshell gene transcription factor into the knockout construct.

20. The method of claim 12 wherein step (n) further comprises subcloning cDNA encoding only putative homo-dimerizing and trans-activating domains of said transcription factor into the knockout construct.

21. The method of claim 12 wherein:
step (m) further comprises identifying a vitelline-specific gene, wherein said gene encodes a vitelline specific structural protein such as an eggshell protein, or a vitelline specific enzyme, or a vitelline specific transcription factor; and step (n) further comprises subcloning said eggshell knockout construct into a plasmid containing local upstream and downstream promoter/enhancer sequences and 5' and 3' untranslated sequences derived from said vitelline-specifiic gene.

22. The method of claim 1 wherein said stage I
schistosome eggs contain zygotes harboring a transgene encoding a surrogate vitelline gland transcription factor produced by the steps of:
l. identifying a vitelline gland-specific transcription factor gene, including its DNA-binding domain and other domains;
m. identifying a gene for a non-schistosomal transcription factor with a different sequence specificity;
n. creating a surrogate transcription factor expression construct by subcloning the cDNA encoding the DNA-binding domain of said non-schistosomal transcription factor in frame with the other domains of the vitelline-specific transcription factor, into a plasmid containing local upstream and downstream poromoter/enhancer sequences, and containing 5' and 3' untranslated regions of a gene expressed in the vitelline gland;
o. microinjecting said knockout construct into the pronuclei or cytoplasm of the zygotes of stage I schistosome eggs;
p. culturing said schistosome eggs to maturity in vitro;
q. allowing miracidia to hatch;
r. infecting snails on a one-miracidium-per-snail basis;
s. culturing said snails until cercaria of schistosome clones are produced;
t. identifying at the cercarial stage clones carrying said knockout-construct;
u. maintaining said knockout-construct-carrying-clones by sporocyst transfer;
v. infecting an animal host with said cercaria of said knockout-construct-carrying-clones; and w. harvesting stage I eggs of said knockout-construct-carrylng-clones.

23. The method of claim 21 wherein step (n) further comprises subcloning binding sites for said surrogate transcription factor into the promoter/enhancer regions of said surrogate transcription factor expression construct.

24. The method of claim 21 wherein step (n) further comprises identifying putative functional areas of said promoter/enhancer regions by interspecies or interstrain sequence conservation or DNAse protection or gel retardation assays, and deleting from said plasmid segments between functional areas.

25. The method of claim 21 wherein step (n) further comprises creating plasmid subclones with progressive unidirectional or nested deletions of said promoter/enhancer regions of said plasmid.

26. The method of claim 21 wherein step (n) further comprises subcloning locus control region elements flanking said promoter/ enhancer regions of said plasmid.

27. The method of claim 21 wherein step (n) further comprises subcloning boundary domain elements flanking said promoter/enhancer regions of said plasmid.

28. The method of claim 21 wherein, in step (n), said gene expressed in the vitelline gland comprises a gene encoding an eggshell protein or a vitelline-specific transcription factor.

29. The method of claim 1 wherein step (b) further comprises subcloning binding sites for a nonschistosomal transcription factor into the promoter/enhancer regions of said schistosome gene.

30. A method for producing therapeutic gene products in a mammal using transgenic schistosomes comprising:
a. identifying a gene whose protein expression is desired;
b. subcloning a protein-coding portion of a cDNA from said gene into a plasmid containing local upstream and downstream promoter/enhancer sequences, and containing 5' and 3' untranslated regions of a schistosome gene expressed in the integument, vitelline or Mehlis glands, or ootype of schistosomes;
c. microinjecting the transgene DNA into the pronuclei or cytoplasm of the zygotes of stage I schistosome eggs;
d. culturing said schistosome eggs to maturity in vitro;
e. allowing miracidia to hatch;

f. injecting snails on a one-miracidium-per-snail basis;
g. culturing said snails until cercaria of schistosome clones are produced;
h. identifying transgenic schistosome clones at the cercarial stage;
i. maintaining said transgenic schistosome clones by sporocyst transfer;
j. infecting a mammal capable of serving as a schistosome host with said cercaria of said transgenic schistosome clones on a one-clone-per-animal basis; and k. allowing said transgenic schistosome clones to secrete transgene product into peripheral blood of said mammal.

31. A method of producing schistosome eggs whose zygotes harbor eggshell knockout constructs, for subsequent use in a method for production of transgenic schistosomes for secretion of desired gene products, said method comprising:
a. identifying a target gene, being a schistosome eggshell gene, the gene encoding a positive schistosome eggshell gene transcription factor, or the gene encoding an enzyme required for maturation of the schistosome eggshell;
b. identifying a ubiquitously expressed schistosome gene;
c. creating an eggshell knockout construct by subcloning an eggshell knockout cDNA into a plasmid containing local upstream and downstream promoter/enhancer sequences and 5' and 3' untranslated sequences derived from said ubiquitously expressed schistosome gene, from said schistosome eggshell gene or from said eggshell gene transcription factor;
d. microinjecting said knockout construct into the pronuclei or cytoplasm of the zygotes of stage I schistosome eggs;
e. culturing said schistosome eggs to maturity in vitro;
f. allowing miracidia to hatch;
g. infecting snails on a one-miracidium-per-snail basis;

h. culturing said snails until cercaria of schistosome clones are produced;
i. identifying at the cercarial stage clones carrying said knockout-construct;
j. maintaining said knockout-construct-carrying-clones by sporocyst transfer;
k. infecting an animal host with said cercaria of said knockout-construct-carrying-clones; and l. harvesting stage I eggs of said knockout-construct-carrying-clones.

32. The method of claim 31 wherein said method for production of transgenic schistosomes for secretion of desired gene products comprises:
a. identifying a gene whose expression is desired;
b. subcloning a protein-coding portion of a cDNA from said gene into a plasmid containing local upstream and downstream promoter/enhancer sequences, and containing 5' and 3' untranslated regions of a schistosome gene expressed in the integument, vitelline or Mehlis glands, or ootype of schistosomes;
c. microinjecting the transgene DNA into the pronuclei or cytoplasm of the zygotes of said stage I schistosome eggs of said knockout-construct-carrying-clones;
d. culturing said schistosome eggs to maturity in vitro;
e. allowing miracidia to hatch;
f. injecting snails on a one-miracidium-per-snail basis;
g. culturing said snails until cercaria of schistosome clones are produced;
h. identifying transgenic schistosome clones at the cercarial stage;
i. maintaining said transgenic schistosome clones by sporocyst transfer;
j. injecting an animal host with said cercaria of said transgenic schistosome clones on a one-clone-per-animal basis and k. identifying transgenic schistosome clones secreting high levels of transgene product into peripheral blood of said animal host.

33. A method of creating schistosome eggs whose zygotes harbor a transgene encoding a surrogate vitelline gland transcription factor, for subsequent use a method for production of transgenic schistosomes for secretion of desired gene products, said method comprising:
a. identifying a vitelline gland-specific transcription factor gene, including its DNA-binding domain and other domains;
b. identifying a gene for a non-schistosomal transcription factor with a different sequence specificity;
c. creating a surrogate transcription factor expression construct by subcloning the cDNA encoding the DNA-binding domain of said non-schistosomal transcription factor in frame with the other domains of the vitelline-specific transcription factor, into a plasmid containing local upstream and downstream poromoter/enhancer sequences, and containing 5' and 3' untranslated regions of a gene expressed in the vitelline gland;
d. microinjecting said knockout construct into the pronuclei or cytoplasm of the zygotes of stage I schistosome eggs;
e. culturing said schistosome eggs to maturity in vitro;
f. allowing miracidia to hatch;
g. injecting snails on a one-miracidium-per-snail basis;
h. culturing said snails until cercaria of schistosome clones are produced;
i. identifying at the cercarial stage clones carrying said knockout-construct;
j. maintaining said knockout-construct-carrying-clones by sporocyst transfer;
k. injecting an animal host with said cercaria of said knockout-construct-carrying-clones; and l. harvesting stage I eggs of said knockout-construct-carrying-clones.

34. The method of claim 33 wherein said method for production of transgenic schistosomes for secretion of desired gene products comprises:
a. identifying a gene whose expression is desired;
b. subcloning a protein-coding portion of a cDNA from said gene into a plasmid containing local upstream and downstream promoter/enhancer sequences, containing 5' and 3' untranslated regions of a schistosome gene expressed in the integument, vitelline or Mehlis glands, or ootype of schistosomes, and containing in the promoter region binding sites for said surrogate transcription factor;
c. microinjecting the transgene DNA into the pronuclei or cytoplasm of the zygotes of said stage I schistosome eggs of said knockout-construct-carrying-clones;
d. culturing said schistosome eggs to maturity in vitro;
e. allowing miracidia to hatch;
f. infecting snails on a one-miracidium-per-snail basis;
g. culturing said snails until cercaria of schistosome clones are produced;
h. identifying transgenic schistosome clones at the cercarial stage;
i. maintaining said transgenic schistosome clones by sporocyst transfer;
j. infecting an animal host with said cercaria of said transgenic schistosome clones on a one-clone-per-animal basis;
and k. identifying transgenic schistosome clones secreting high levels of transgene product into peripheral blood of said animal host.

35. A method for producing transgenic schistosomes for secretion of desired gene products comprising:
a. identifying a gene whose expression is desired;
b. subcloning a protein-coding portion of a cDNA from said gene into a plasmid containing local upstream and downstream promoter/enhancer sequences, and containing 5' and 3' untranslated regions of a schistosome gene expressed in the integument, vitelline or Mehlis glands, or ootype of schistosomes;
c. microinjecting the transgene DNA into the pronuclei or cytoplasm of the zygotes of stage I schistosome eggs containing zygotes harboring eggshell knockout constructs and a surrogate vitelline gland transcription factor;
d. culturing said schistosome eggs to maturity in vitro;
e. allowing miracidia to hatch;
f. infecting snails on a one-miracidium-per-snail basis;
g. culturing said snails until cercaria of schistosome clones are produced;
f. identifying transgenic schistosome clones at the cercarial stage;
g. maintaining said transgenic schistosome clones by sporocyst transfer;
h. infecting an animal host with said cercaria of said transgenic schistosome clones on a one-clone-per-animal basis;
and i. identifying transgenic schistosome clones secreting high levels of transgene product into peripheral blood of said animal host.

36. The method of claim 35 wherein said stage I
schistosome eggs of step (c) are produced by the steps of:
j. identifying a target gene, being a schistosome eggshell gene, the gene encoding a putative transcription factor that up-regulates a schistosome eggshell gene, or the gene encoding an enzyme required for maturation of the schistosome eggshell;
k. identifying a ubiquitously expressed schistosome gene;
l. creating an eggshell knockout construct by subcloning an eggshell knockout cDNA into a plasmid containing local upstream and downstream promoter/enhancer sequences and 5' and 3' untranslated sequences derived from said ubiquitously expressed schistosome gene, from said schistosome eggshell gene or from said eggshell gene transcription factor;

m. microinjecting said knockout construct into the pronuclei or cytoplasm of the zygotes of stage I schistosome eggs;
n. culturing said schistosome eggs to maturity in vitro;
o. allowing miracidia to hatch;
p. injecting snails on a one-miracidium-per-snail basis;
q. culturing said snails until cercaria of schistosome clones are produced;
r. identifying at the cercarial stage clones carrying said knockout-construct;
s. determining the gender of said schistosome clones carrying said knockout construct;
t. maintaining said knockout-construct-carrying-clones by sporocyst transfer;
u. co-injecting an animal host with said cercaria of said knockout-construct-carrying-clones; and v. harvesting stage I eggs of said knockout-construct-carrying-clones.

37. The method of claim 36 further comprising, prior to step (n) of claim 36, the steps of:
(1) identifying a vitelline gland-specific transcription factor gene, including its DNA-binding domain and other domains;
(2) identifying a gene for a non-schistosomal transcription factor with a different sequence specificity;
(3) creating a surrogate transcription factor expression construct by subcloning the cDNA encoding the DNA-binding domain of said non-schistosomal transcription factor in frame with the other domains of the vitelline-specific transcription factor, into a plasmid containing local upstream and downstream poromoter/enhancer sequences, and containing 5' and 3' untranslated regions of a gene expressed in the vitelline gland; and (4) microinjecting said knockout construct into the pronuclei or cytoplasm of the zygotes of stage I schistosome eggs.