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CA2215650A1 - Dna microwell device and method - Google Patents

Dna microwell device and method

Info

Publication number
CA2215650A1
CA2215650A1 CA2215650A CA2215650A CA2215650A1 CA 2215650 A1 CA2215650 A1 CA 2215650A1 CA 2215650 A CA2215650 A CA 2215650A CA 2215650 A CA2215650 A CA 2215650A CA 2215650 A1 CA2215650 A1 CA 2215650A1
Authority
CA
Canada
Prior art keywords
sample
nucleic acid
liquid biological
biological sample
sample well
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA2215650A
Other languages
French (fr)
Other versions
CA2215650C (en
Inventor
Hugh V. Cottingham
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Becton Dickinson and Co
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2215650A1 publication Critical patent/CA2215650A1/en
Application granted granted Critical
Publication of CA2215650C publication Critical patent/CA2215650C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5085—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01—Arrangements or apparatus for facilitating the optical investigation
    • G01N21/03—Cuvette constructions
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01—Arrangements or apparatus for facilitating the optical investigation
    • G01N21/03—Cuvette constructions
    • G01N2021/0346—Capillary cells; Microcells

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

An apparatus for carrying out a homogeneous nucleic acid amplification and nucleic acid assay on a liquid biological sample comprises a sample well andan optical window element which is received in the sample well. Opposed, spaced-apart surfaces of the optical window element and sample well define a capillary chamber into which a liquid biological sample is drawn by capillary force. By spreading the liquid biological sample into a thin film within the capillary chamber, head space is eliminated, heat transfer to the sample is maximized, and a large optical target is achieved to facilitate the detection step of the assay. The disclosed apparatus is particularly suited for use with homogeneous nucleic acid amplification and fluorescence polarization assays, butcan also be used in connection with other types of biological and chemical processes.
CA002215650A 1996-09-26 1997-09-15 Dna microwell device and method Expired - Fee Related CA2215650C (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/721,372 1996-09-26
US08/721,372 US5795748A (en) 1996-09-26 1996-09-26 DNA microwell device and method

Publications (2)

Publication Number Publication Date
CA2215650A1 true CA2215650A1 (en) 1998-03-26
CA2215650C CA2215650C (en) 2000-04-04

Family

ID=24897713

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002215650A Expired - Fee Related CA2215650C (en) 1996-09-26 1997-09-15 Dna microwell device and method

Country Status (6)

Country Link
US (1) US5795748A (en)
EP (1) EP0834729A3 (en)
JP (1) JPH10127268A (en)
AU (1) AU719489B2 (en)
BR (1) BR9704776A (en)
CA (1) CA2215650C (en)

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US7842499B2 (en) * 2006-08-07 2010-11-30 Platypus Technologies, Llc Substrates, devices, and methods for cellular assays
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JP5878254B2 (en) * 2015-03-03 2016-03-08 ヤマハ発動機株式会社 Well plate and suction device provided with the well plate
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Also Published As

Publication number Publication date
AU3927897A (en) 1998-04-02
EP0834729A2 (en) 1998-04-08
US5795748A (en) 1998-08-18
BR9704776A (en) 1998-12-29
JPH10127268A (en) 1998-05-19
EP0834729A3 (en) 1998-10-21
CA2215650C (en) 2000-04-04
AU719489B2 (en) 2000-05-11

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Legal Events

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