CA2211165A1 - Methods for sensitive detection of reverse transcriptase - Google Patents
Methods for sensitive detection of reverse transcriptaseInfo
- Publication number
- CA2211165A1 CA2211165A1 CA002211165A CA2211165A CA2211165A1 CA 2211165 A1 CA2211165 A1 CA 2211165A1 CA 002211165 A CA002211165 A CA 002211165A CA 2211165 A CA2211165 A CA 2211165A CA 2211165 A1 CA2211165 A1 CA 2211165A1
- Authority
- CA
- Canada
- Prior art keywords
- reverse transcriptase
- dna
- biological sample
- methods
- synthesized
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 102100034343 Integrase Human genes 0.000 title abstract 3
- 108010092799 RNA-directed DNA polymerase Proteins 0.000 title abstract 3
- 238000000034 method Methods 0.000 title abstract 2
- 238000011896 sensitive detection Methods 0.000 title 1
- 108020004414 DNA Proteins 0.000 abstract 4
- 239000012472 biological sample Substances 0.000 abstract 4
- 108020001019 DNA Primers Proteins 0.000 abstract 2
- 239000003155 DNA primer Substances 0.000 abstract 2
- 230000003321 amplification Effects 0.000 abstract 2
- 238000003199 nucleic acid amplification method Methods 0.000 abstract 2
- 241001430294 unidentified retrovirus Species 0.000 abstract 2
- 108020004635 Complementary DNA Proteins 0.000 abstract 1
- 102000053602 DNA Human genes 0.000 abstract 1
- 238000010804 cDNA synthesis Methods 0.000 abstract 1
- 239000002299 complementary DNA Substances 0.000 abstract 1
- 239000013615 primer Substances 0.000 abstract 1
- 239000000523 sample Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
- C12Q1/701—Specific hybridization probes
- C12Q1/702—Specific hybridization probes for retroviruses
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Immunology (AREA)
- Virology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The present invention provides a method for detecting the presence of a retrovirus in a biological sample comprising the steps of : a) contacting the biological sample with an RNA template and a complementary DNA primer under conditions whereby the RNA template and the DNA primer will anneal and a DNA
strand will be synthesized as an extension from the DNA primer if reverse transcriptase is present in the sample; b) amplifying the synthesized DNA; and c) detecting the amplification of the synthesized DNA, the amplification of the synthesized DNA indicating the presence of reverse transcriptase in the biological sample, thus indicating the presence of a retrovirus in the biological sample.
strand will be synthesized as an extension from the DNA primer if reverse transcriptase is present in the sample; b) amplifying the synthesized DNA; and c) detecting the amplification of the synthesized DNA, the amplification of the synthesized DNA indicating the presence of reverse transcriptase in the biological sample, thus indicating the presence of a retrovirus in the biological sample.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US37985195A | 1995-01-27 | 1995-01-27 | |
| US08/379,851 | 1995-01-27 | ||
| PCT/US1996/001257 WO1996023076A1 (en) | 1995-01-27 | 1996-01-26 | Methods for sensitive detection of reverse transcriptase |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2211165A1 true CA2211165A1 (en) | 1996-08-01 |
| CA2211165C CA2211165C (en) | 2006-10-24 |
Family
ID=37309488
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002211165A Expired - Fee Related CA2211165C (en) | 1995-01-27 | 1996-01-26 | Methods for sensitive detection of reverse transcriptase |
Country Status (1)
| Country | Link |
|---|---|
| CA (1) | CA2211165C (en) |
-
1996
- 1996-01-26 CA CA002211165A patent/CA2211165C/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| CA2211165C (en) | 2006-10-24 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request | ||
| MKLA | Lapsed |
Effective date: 20160126 |