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CA2196410A1 - Control of plant gene expression - Google Patents

Control of plant gene expression

Info

Publication number
CA2196410A1
CA2196410A1 CA002196410A CA2196410A CA2196410A1 CA 2196410 A1 CA2196410 A1 CA 2196410A1 CA 002196410 A CA002196410 A CA 002196410A CA 2196410 A CA2196410 A CA 2196410A CA 2196410 A1 CA2196410 A1 CA 2196410A1
Authority
CA
Canada
Prior art keywords
plant
gene
promoter
specific
sequences
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002196410A
Other languages
French (fr)
Other versions
CA2196410C (en
Inventor
Melvin John Oliver
Jerry Edwin Quisenberry
Norma Lee Glover Trolinder
Don Lee Keim
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
US Department of Agriculture USDA
D&PL Technology Holding Co LLC
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US08/477,559 external-priority patent/US5723765A/en
Application filed by Individual filed Critical Individual
Publication of CA2196410A1 publication Critical patent/CA2196410A1/en
Application granted granted Critical
Publication of CA2196410C publication Critical patent/CA2196410C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8287—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for fertility modification, e.g. apomixis
    • C12N15/8289—Male sterility
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8217—Gene switch

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Botany (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)

Abstract

A method for making a genetically modified plant comprises regenerating a whole plant from a plant cell that has been transfected with DNA sequences comprising a first gene whose expression result in an altered plant phenotype linked to a transiently active promoter, the gene and promoter being separated by a blocking sequence flanked by specific excision sequences, a second gene that encodes a recombinase specific for the specific excision sequences linked to a repressible promoter, and a third gene that encodes the repressor specific for the repressible promoter. Also, a genetically modified hybrid plant is made by hybridizing a first plant regenerated from a plant cell that has been transfected with DNA
sequences comprising a first gene whose expression results in an altered plant phenotype linked to a transiently active promoter, the gene and promoter being separated by a blocking sequence flanked by specific excision sequences to a second plant regenerated from a second plant cell that has been transfected with DNA sequences comprising a second gene that encodes a recombinase specific for the specific excision sequences linked to a promoter that is active during seed germination, and growing a hybrid plant from the hybrid seed. Plant cells, plant tissues, plant seed and whole plants containing the above DNA sequences are also claimed.
CA002196410A 1994-08-01 1995-07-31 Control of plant gene expression Expired - Fee Related CA2196410C (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US28360494A 1994-08-01 1994-08-01
US08/283,604 1994-08-01
US08/477,559 US5723765A (en) 1994-08-01 1995-06-07 Control of plant gene expression
US08/477,559 1995-06-07
PCT/US1995/009595 WO1996004393A2 (en) 1994-08-01 1995-07-31 Control of plant gene expression

Publications (2)

Publication Number Publication Date
CA2196410A1 true CA2196410A1 (en) 1996-02-15
CA2196410C CA2196410C (en) 2005-10-11

Family

ID=26962145

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002196410A Expired - Fee Related CA2196410C (en) 1994-08-01 1995-07-31 Control of plant gene expression

Country Status (15)

Country Link
US (2) US5925808A (en)
EP (1) EP0775212B1 (en)
JP (1) JPH10503377A (en)
CN (1) CN1112445C (en)
AT (1) ATE305976T1 (en)
AU (1) AU696668B2 (en)
BR (1) BR9508471A (en)
CA (1) CA2196410C (en)
DE (1) DE69534503T2 (en)
DK (1) DK0775212T3 (en)
EG (1) EG23907A (en)
ES (1) ES2250974T3 (en)
HK (1) HK1003271A1 (en)
TR (1) TR199500933A2 (en)
WO (1) WO1996004393A2 (en)

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Also Published As

Publication number Publication date
CN1161717A (en) 1997-10-08
ATE305976T1 (en) 2005-10-15
AU696668B2 (en) 1998-09-17
DE69534503T2 (en) 2006-07-06
AU3205095A (en) 1996-03-04
EG23907A (en) 2007-12-30
CN1112445C (en) 2003-06-25
WO1996004393A2 (en) 1996-02-15
ES2250974T3 (en) 2006-04-16
CA2196410C (en) 2005-10-11
BR9508471A (en) 1997-10-28
US5977441A (en) 1999-11-02
HK1003271A1 (en) 1998-10-23
DK0775212T3 (en) 2006-02-20
EP0775212B1 (en) 2005-10-05
DE69534503D1 (en) 2006-02-16
TR199500933A2 (en) 1996-07-21
JPH10503377A (en) 1998-03-31
WO1996004393A3 (en) 1996-03-07
EP0775212A2 (en) 1997-05-28
US5925808A (en) 1999-07-20

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