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CA2121121A1 - Preparation of recombinant borrelia proteins - Google Patents

Preparation of recombinant borrelia proteins

Info

Publication number
CA2121121A1
CA2121121A1 CA002121121A CA2121121A CA2121121A1 CA 2121121 A1 CA2121121 A1 CA 2121121A1 CA 002121121 A CA002121121 A CA 002121121A CA 2121121 A CA2121121 A CA 2121121A CA 2121121 A1 CA2121121 A1 CA 2121121A1
Authority
CA
Canada
Prior art keywords
protein
gene
ospa
encoded
detergent phase
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002121121A
Other languages
French (fr)
Other versions
CA2121121C (en
Inventor
Lorne F. Erdile
Mary-Ann Brandt
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Sanofi Pasteur Inc
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2121121A1 publication Critical patent/CA2121121A1/en
Application granted granted Critical
Publication of CA2121121C publication Critical patent/CA2121121C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/20—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Spirochaetales (O), e.g. Treponema, Leptospira
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/04—Antibacterial agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Communicable Diseases (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Oncology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Genetics & Genomics (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Highly-purified immunologically-effected recombinant protein encoded by a gene of a full-length wild-type Borrelia lipoprotein, particularly the OspA encoded by the ospA gene of B.burgdorferi, useful in vaccines and test kits for Lyme disease, is prepared by inducing protein production from a host organism transformed by a plasmid vector containing the cloned gene and subsequently recovering and purifying the protein. The grown organism is lysed and contacted with a surfactant, particularly Triton X-114, which selectively extracts the desired protein from the lysed cells. Upon heating the resulting mixture to mildly-elevated temperature, a detergent phase separates out and is recovered separate from the other phases. The detergent phase is further purified from residual protein by column chromatography.
CA002121121A 1991-10-18 1992-10-16 Preparation of recombinant borrelia proteins Expired - Fee Related CA2121121C (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US77904891A 1991-10-18 1991-10-18
US779,048 1991-10-18
US88876592A 1992-05-27 1992-05-27
US888,765 1992-05-27
PCT/US1992/008697 WO1993008299A1 (en) 1991-10-18 1992-10-16 Preparation of recombinant borrelia proteins

Publications (2)

Publication Number Publication Date
CA2121121A1 true CA2121121A1 (en) 1993-04-29
CA2121121C CA2121121C (en) 2003-06-10

Family

ID=27119531

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002121121A Expired - Fee Related CA2121121C (en) 1991-10-18 1992-10-16 Preparation of recombinant borrelia proteins

Country Status (9)

Country Link
EP (1) EP0620860A4 (en)
JP (1) JPH06511154A (en)
AU (1) AU676140B2 (en)
CA (1) CA2121121C (en)
FI (1) FI941749L (en)
IL (1) IL103462A0 (en)
NO (1) NO310417B1 (en)
PT (1) PT100980B (en)
WO (1) WO1993008299A1 (en)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE4015911A1 (en) * 1989-09-19 1991-03-28 Max Planck Gesellschaft VACCINE AGAINST LYME DISEASE
US6676942B1 (en) 1991-08-15 2004-01-13 Smithkline Beecham Biologicals (S.A.) Osp a proteins of Borrelia burgdorferi subgroups, encoding genes and vaccines
EP0878545A3 (en) * 1991-08-15 1998-12-16 SMITHKLINE BEECHAM BIOLOGICALS s.a. OSP a protein of borrelia burgdorferi subgroups, encoding genes and vaccines
CA2105629A1 (en) * 1992-09-14 1994-03-15 Robert S. Becker Potentiation of immunogenic response
US7008625B2 (en) 1993-11-01 2006-03-07 Research Foundation Of The State University Of New York Recombinant constructs of Borrelia burgdorferi
US6248562B1 (en) 1993-11-01 2001-06-19 Research Foundation State University Of New York Chimeric proteins comprising borrelia polypeptides and uses therefor
US6610838B1 (en) 1997-09-10 2003-08-26 Symbicom Aktiebolag P13 antigens from Borrelia
US6224882B1 (en) 1997-11-07 2001-05-01 Protein Science Corp. Insect cells or fractions as adjuvant for antigens
GB9811219D0 (en) * 1998-05-26 1998-07-22 Smithkline Beecham Biolog Novel process
AU4654500A (en) * 1999-04-21 2000-11-02 Boston Medical Center Corporation Prevention, diagnosis and treatment of lyme disease
DE60131982T2 (en) 2000-08-18 2008-12-11 Brookhaven Science Associates Llc CHANGED BORRELIA BURGDORFERI OSPA

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK590288D0 (en) * 1988-10-24 1988-10-24 Symbicom Ab CHEMICAL COMPOUNDS
DE4015911A1 (en) * 1989-09-19 1991-03-28 Max Planck Gesellschaft VACCINE AGAINST LYME DISEASE
ATE140461T1 (en) * 1989-12-22 1996-08-15 Mikrogen Molekularbiol Entw IMMUNOLOGICALLY ACTIVE PROTEINS OF BORRELIA BURGDORFERI, RELATED TEST KITS AND VACCINE
CA2057536C (en) * 1990-12-21 1999-10-26 John J. Dunn Cloning and expression of borrelia lipoproteins
EP0878545A3 (en) * 1991-08-15 1998-12-16 SMITHKLINE BEECHAM BIOLOGICALS s.a. OSP a protein of borrelia burgdorferi subgroups, encoding genes and vaccines

Also Published As

Publication number Publication date
PT100980A (en) 1994-01-31
WO1993008299A1 (en) 1993-04-29
NO941376L (en) 1994-06-17
AU676140B2 (en) 1997-03-06
FI941749L (en) 1994-05-25
AU2801192A (en) 1993-05-21
PT100980B (en) 1999-07-30
EP0620860A1 (en) 1994-10-26
NO941376D0 (en) 1994-04-15
EP0620860A4 (en) 1997-05-02
CA2121121C (en) 2003-06-10
NO310417B1 (en) 2001-07-02
JPH06511154A (en) 1994-12-15
FI941749A0 (en) 1994-04-15
IL103462A0 (en) 1993-03-15

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