CA2080684A1 - Bacillus thuringiensis cryif and cryix genes and proteins toxic to lepidopteran insects - Google Patents
Bacillus thuringiensis cryif and cryix genes and proteins toxic to lepidopteran insectsInfo
- Publication number
- CA2080684A1 CA2080684A1 CA002080684A CA2080684A CA2080684A1 CA 2080684 A1 CA2080684 A1 CA 2080684A1 CA 002080684 A CA002080684 A CA 002080684A CA 2080684 A CA2080684 A CA 2080684A CA 2080684 A1 CA2080684 A1 CA 2080684A1
- Authority
- CA
- Canada
- Prior art keywords
- bacterium
- gene
- lepidopteran
- protein
- bacillus thuringiensis
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/32—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
- C07K14/325—Bacillus thuringiensis crystal peptides, i.e. delta-endotoxins
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/50—Isolated enzymes; Isolated proteins
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Biophysics (AREA)
- Pest Control & Pesticides (AREA)
- Molecular Biology (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- Gastroenterology & Hepatology (AREA)
- Agronomy & Crop Science (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Plant Pathology (AREA)
- Virology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Dentistry (AREA)
- Wood Science & Technology (AREA)
- Environmental Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Abstract
Two purified and isolated cryI-type genes were obtained from a novel B.t. strain. One gene, designated cryIF, has a nucleotide base sequence coding for the amino acid sequence illustrated in Figure 1. The 134 kDa crystal protein, designated CryIF, produced by this gene is toxic to European corn borer larvae and other lepidopteran insects.
The second gene, designated cryIX, produces a crystal protein of about 81 kDa, designated CryIX, that is also toxic to lepidopteran insects.
The second gene, designated cryIX, produces a crystal protein of about 81 kDa, designated CryIX, that is also toxic to lepidopteran insects.
Claims (33)
1. A purified and isolated cryIF gene characterized by a nucleotide base sequence coding for the amino acid sequence illustrated in Figure 1 (SEQ ID NO:2).
2. A purified and isolated cryIF gene according to claim 1 further characterized in that the gene has a coding region extending from nucleotide bases 478 to 3999 in the nucleotide base sequence illustrated in Figure 1 (SEQ ID NO:1).
3. A lepidopteran-toxic protein characterized in that the protein is produced by the gene of claim 1 or claim 2.
4. A recombinant plasmid characterized in that the recombinant plasmid contains the gene of claim 1 or claim 2.
5. A biologically pure culture of a bacterium characterized in that the bacterium is transformed with the recombinant plasmid of claim 4.
6. The bacterium of claim 5 further characterized in that the bacterium is E. coli.
7. The E. coli bacterium of claim 6 further characterized in that the bacterium is deposited with the NRRL with accession number NRRL B-18634.
8. The bacterium of claim 5 further characterized in that the bacterium is Bacillus thuringiensis.
9. The Bacillus thuringiensis bacterium of claim 8 further characterized in that the bacterium is deposited with the NRRL with accession number NRRL B-18635.
10. An insecticide composition characterized in that the composition contains the protein of claim 3 and an agriculturally acceptable carrier.
11. An insecticide composition characterized in that the composition contains the bacterium of claim 5, a lepidopteran-toxic protein produced by such bacterium, and an aqriculturally acceptable carrier.
12. A lepidopteran-toxic protein characterized by the protein being characteristic of that made by the Bacillus thuringiensis bacterium of claim 9 and having the amino acid sequence illustrated in Figure 1 (SEQ ID N0:2).
13. An insecticide composition characterized in that the composition contains the lepidopteran-toxic protein of claim 12, in combination with an agriculturally acceptable carrier.
14. The insecticide composition of claim 13 further characterized in that the lepidopteran-toxic protein is contained in a Bacillus thuringiensis bacterium.
15. A method of controlling lepidopteran insects characterized by applying to a host plant for such insects an insecticidally effective amount of the lepidopteran-toxic protein of claim 3.
16. The method of claim 15 further characterized in that the lepidopteran-toxic protein is contained in a Bacillus thuringiensis bacterium.
17. A method of controlling lepidopteran insects characterized by applying to a host plant for such insects an insecticidally effective amount of the lepidopteran-toxic protein of claim 12.
18. The method of claim 17 further characterized in that the lepidopteran-toxic protein is contained in a Bacillus thuringiensis bacterium.
19. A plant characterized in that the plant is transformed with the gene of claim 1 or claim 2.
20. The cryIF gene of claim 2 further characterized in that the gene or a portion thereof is labeled for use as a hybridization probe.
21. A biologically pure culture of a Bacillus thuringiensis bacterium characterized in that the culture is deposited with the NRRL with accession number NRRL B-18633.
22. A purified and isolated cryIX gene characterized by the gene being characteristic of that contained in E. coli strain EG1083 deposited with the NRRL with accession number NRRL B-18805.
23. A lepidopteran-toxic protein characterized in that the protein is produced by the gene of claim 22 and has a molecular mass of about 81 kDa.
24. A recombinant plasmid characterized in that the recombinant plasmid contains the gene of claim 22.
25. A biologically pure culture of a bacterium characterized in that the bacterium is transformed with the recombinant plasmid of claim 24.
26. The bacterium of claim 25 further characterized in that the bacterium is selected from the group consisting of E. coli and Bacillus thuringiensis.
27. An E. coli bacterium according to claim 26 further characterized in that the bacterium is deposited with the NRRL with accession number NRRL B-18805.
28. An insecticide composition characterized in that the composition contains the protein of claim 23 and an agriculturally acceptable carrier.
29. An insecticide composition characterized in that the composition contains the bacterium of claim 25, an insecticidal protein produced by such bacterium, and an agriculturally acceptable carrier.
30. A method of controlling lepidopteran insects characterized by applying to a host plant for such insects an insecticidally effective amount of the lepidopteran-toxic protein of claim 23.
31. The method of claim 30 further characterized in that the lepidopteran-toxic protein is contained in a Bacillus thuringiensis bacterium.
32. A plant characterized in that the plant is transformed with the gene of claim 22.
33. The cryIX gene of claim 22 characterized in that the gene or a portion thereof is labeled for use as a hybridization probe.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US51032790A | 1990-04-16 | 1990-04-16 | |
| US510,327 | 1990-04-16 | ||
| PCT/US1991/002560 WO1991016434A2 (en) | 1990-04-16 | 1991-04-15 | BACILLUS THURINGIENSIS cryIF AND cryIX GENES AND PROTEINS TOXIC TO LEPIDOPTERAN INSECTS |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2080684A1 true CA2080684A1 (en) | 1991-10-17 |
| CA2080684C CA2080684C (en) | 2010-02-02 |
Family
ID=24030295
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002080684A Expired - Lifetime CA2080684C (en) | 1990-04-16 | 1991-04-15 | Bacillus thuringiensis cryif and cryix genes and proteins toxic to lepidopteran insects |
Country Status (5)
| Country | Link |
|---|---|
| EP (1) | EP0528823A1 (en) |
| AU (1) | AU647121B2 (en) |
| CA (1) | CA2080684C (en) |
| TR (1) | TR26973A (en) |
| WO (1) | WO1991016434A2 (en) |
Families Citing this family (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5686069A (en) * | 1990-10-15 | 1997-11-11 | Mycogen Corporation | Protein toxins active against lepidopteran pests |
| TW261517B (en) * | 1991-11-29 | 1995-11-01 | Mitsubishi Shozi Kk | |
| US5356623A (en) * | 1993-03-17 | 1994-10-18 | Ecogen Inc. | Bacillus thuringiensis cryET1 toxin gene and protein toxic to lepidopteran insects |
| HU220714B1 (en) * | 1993-03-25 | 2002-04-29 | Novartis Ag. | New pesticide proteins and strains |
| US5877012A (en) * | 1993-03-25 | 1999-03-02 | Novartis Finance Corporation | Class of proteins for the control of plant pests |
| US5849870A (en) * | 1993-03-25 | 1998-12-15 | Novartis Finance Corporation | Pesticidal proteins and strains |
| US5322687A (en) * | 1993-07-29 | 1994-06-21 | Ecogen Inc. | Bacillus thuringiensis cryet4 and cryet5 toxin genes and proteins toxic to lepidopteran insects |
| US5508264A (en) * | 1994-12-06 | 1996-04-16 | Mycogen Corporation | Pesticidal compositions |
| ATE443437T1 (en) * | 1995-10-13 | 2009-10-15 | Dow Agrosciences Llc | MODIFIED BACILLUS THURINGIENSIS GENE FOR CONTROL OF LEPIDOPTERA IN PLANTS |
| KR20000022459A (en) | 1996-07-01 | 2000-04-25 | 칼튼 제이. 에이블 | Toxins active against pests |
| US6369213B1 (en) * | 1996-07-01 | 2002-04-09 | Mycogen Corporation | Toxins active against pests |
| US5965428A (en) * | 1996-10-08 | 1999-10-12 | Ecogen, Inc. | Chimeric lepidopteran-toxic crystal proteins |
| AU727218B2 (en) | 1997-04-03 | 2000-12-07 | Syngenta Participations Ag | Plant pest control |
| AR025349A1 (en) * | 1999-08-23 | 2002-11-20 | Mycogen Corp | METHODS TO CONTROL GRAY WORM PESTS |
| US11130964B2 (en) | 2014-11-20 | 2021-09-28 | Monsanto Technology Llc | Insect inhibitory proteins |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU624349B2 (en) * | 1987-11-05 | 1992-06-11 | David M. Gjerdrum | Device for monitoring radon exposure |
| GB8800652D0 (en) * | 1988-01-13 | 1988-02-10 | Ici Plc | Bacterial strain |
| EP0358557B1 (en) * | 1988-09-06 | 1995-04-05 | Plant Genetic Systems, N.V. | Plants transformed with a DNA sequence from bacillus thuringiensis lethal to lepidoptera |
| GB8910624D0 (en) * | 1989-05-09 | 1989-06-21 | Ici Plc | Bacterial strains |
-
1991
- 1991-04-10 TR TR00366/91A patent/TR26973A/en unknown
- 1991-04-15 WO PCT/US1991/002560 patent/WO1991016434A2/en not_active Application Discontinuation
- 1991-04-15 EP EP91908010A patent/EP0528823A1/en not_active Withdrawn
- 1991-04-15 CA CA002080684A patent/CA2080684C/en not_active Expired - Lifetime
- 1991-04-15 AU AU76873/91A patent/AU647121B2/en not_active Expired
Also Published As
| Publication number | Publication date |
|---|---|
| EP0528823A1 (en) | 1993-03-03 |
| AU647121B2 (en) | 1994-03-17 |
| CA2080684C (en) | 2010-02-02 |
| TR26973A (en) | 1994-09-12 |
| WO1991016434A2 (en) | 1991-10-31 |
| WO1991016434A3 (en) | 1991-12-12 |
| AU7687391A (en) | 1991-11-11 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request | ||
| MKEX | Expiry | ||
| MKEX | Expiry |
Effective date: 20110415 |