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BR9914952A - Process and test kit for DNA repair analysis - Google Patents

Process and test kit for DNA repair analysis

Info

Publication number
BR9914952A
BR9914952A BR9914952-4A BR9914952A BR9914952A BR 9914952 A BR9914952 A BR 9914952A BR 9914952 A BR9914952 A BR 9914952A BR 9914952 A BR9914952 A BR 9914952A
Authority
BR
Brazil
Prior art keywords
test kit
dna repair
dna
repair analysis
repair
Prior art date
Application number
BR9914952-4A
Other languages
Portuguese (pt)
Inventor
Peter Nehls
Karl-Heinz Gluesenkamp
Original Assignee
Peter Nehls
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Peter Nehls filed Critical Peter Nehls
Publication of BR9914952A publication Critical patent/BR9914952A/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Patente de Invenção:<B>''PROCESSO E KIT DE TESTE PARA ANáLISE DE REPARO DE DNA''<D>. A presente invenção refere-se a um processo e a um kit de teste para análise do reparo de modificações no DNA e pareamentos errados de base tais como sítios apurínicos ou apirimidínicos por enzimas de reparo de DNA. De acordo com o processo mencionado, moléculas de DNA que são acopladas covalentemente a uma matriz de fase sólida por meio de reação com um ácido esquárico reativo conduzido em contato com uma composição contendo uma enzima de reparo de DNA. O kit de teste contém os componentes que são requeridos para as ditas análises.Invention Patent: <B> '' PROCESS AND TEST KIT FOR DNA REPAIR ANALYSIS '' <D>. The present invention relates to a process and test kit for analyzing the repair of DNA modifications and erroneous base pairings such as apurinic or apyrimidinic sites by DNA repair enzymes. According to the aforementioned process, DNA molecules that are covalently coupled to a solid phase matrix by reaction with a reactive squaric acid conducted in contact with a composition containing a DNA repair enzyme. The test kit contains the components that are required for said analyzes.

BR9914952-4A 1998-11-03 1999-11-02 Process and test kit for DNA repair analysis BR9914952A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19850680A DE19850680A1 (en) 1998-11-03 1998-11-03 Method for determining the repair capacity of solutions containing DNA repair enzymes and for detecting DNA structural modifications and base mismatches
PCT/EP1999/008365 WO2000026402A1 (en) 1998-11-03 1999-11-02 Method and test kit for analyzing dns repair

Publications (1)

Publication Number Publication Date
BR9914952A true BR9914952A (en) 2001-07-10

Family

ID=7886561

Family Applications (1)

Application Number Title Priority Date Filing Date
BR9914952-4A BR9914952A (en) 1998-11-03 1999-11-02 Process and test kit for DNA repair analysis

Country Status (11)

Country Link
US (1) US20020022228A1 (en)
EP (1) EP1127165A1 (en)
JP (1) JP2002528134A (en)
KR (1) KR20010103630A (en)
CN (1) CN1325456A (en)
AU (1) AU1378300A (en)
BR (1) BR9914952A (en)
CA (1) CA2348937A1 (en)
DE (1) DE19850680A1 (en)
WO (1) WO2000026402A1 (en)
ZA (1) ZA200103497B (en)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2809417B1 (en) * 2000-05-24 2004-07-30 Commissariat Energie Atomique DETECTION AND CHARACTERIZATION OF PROTEIN ACTIVITY INVOLVED IN THE REPAIR OF DNA INJURIES
WO2002065889A1 (en) * 2001-02-21 2002-08-29 Rubikon Ag Method for examining cell and tissue samples
US20020187508A1 (en) * 2001-06-08 2002-12-12 Wong Gordon G. Methods and products for analyzing nucleic acids using nick translation
AU2002367639A1 (en) * 2001-11-14 2003-10-20 Luminex Corporation Functionalized compositions for improved immobilization
FR2849058B1 (en) * 2002-12-20 2005-02-25 Commissariat Energie Atomique METHOD FOR QUANTITATIVE EVALUATION OF GLOBAL AND SPECIFIC DNA REPAIR CAPABILITIES OF AT LEAST ONE BIOLOGICAL MEDIUM, AND ITS APPLICATIONS
FR2887261B1 (en) * 2005-06-20 2007-09-14 Commissariat Energie Atomique METHOD FOR IMMOBILIZATION OF SUPERENROPIC DNA AND USE FOR ANALYZING DNA REPAIR
CN102031285B (en) * 2009-09-28 2016-12-21 复旦大学 A kind of DNA repair ability detection method based on double-core micronucleus
CN118703608A (en) * 2020-11-16 2024-09-27 深圳市真迈生物科技有限公司 Compound modified chip and its preparation method and application
WO2022184907A1 (en) * 2021-03-04 2022-09-09 Lxrepair Multiplex quantitative assay for dna double-strand break repair activities in a biological medium and its applications
CN117466876B (en) * 2023-11-02 2024-09-13 安阳师范学院 Near-infrared small molecule biosensor material for detecting apyrimidine and apurinic sites in tumor cell DNA, and synthesis and application thereof

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE4341524C2 (en) * 1993-12-06 1997-01-16 Gluesenkamp Karl Heinz Dr Process for immobilizing biomolecules and affinity ligands on polymeric supports
FR2731711B1 (en) * 1995-03-15 1997-06-06 Rech Investissements Sfri Soc METHOD FOR QUALITATIVE AND QUANTITATIVE DETECTION OF DNA DAMAGE
DE19624990A1 (en) * 1996-06-22 1998-01-08 Gluesenkamp Karl Heinz Dr Production of polymer compounds with activated amino groups

Also Published As

Publication number Publication date
WO2000026402A1 (en) 2000-05-11
US20020022228A1 (en) 2002-02-21
CA2348937A1 (en) 2000-05-11
ZA200103497B (en) 2002-07-30
AU1378300A (en) 2000-05-22
KR20010103630A (en) 2001-11-23
DE19850680A1 (en) 2000-05-04
JP2002528134A (en) 2002-09-03
EP1127165A1 (en) 2001-08-29
CN1325456A (en) 2001-12-05

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Legal Events

Date Code Title Description
B08F Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette]

Free format text: REFERENTE A 4A, 5A, 6A, 7A E 8A ANUIDADES.

B08K Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette]

Free format text: REFERENTE AO DESPACHO 8.6 PUBLICADO NA RPI 1913 DE 04/09/2007.