[go: up one dir, main page]

BR9811657A - Cloning using donor nuclei from differentiated serum-free cells - Google Patents

Cloning using donor nuclei from differentiated serum-free cells

Info

Publication number
BR9811657A
BR9811657A BR9811657-6A BR9811657A BR9811657A BR 9811657 A BR9811657 A BR 9811657A BR 9811657 A BR9811657 A BR 9811657A BR 9811657 A BR9811657 A BR 9811657A
Authority
BR
Brazil
Prior art keywords
cells
differentiated
serum
production
cloning
Prior art date
Application number
BR9811657-6A
Other languages
Portuguese (pt)
Inventor
Steven L Stice
Jose Cibelli
James M Robl
Paul Golueke
F Abel Ponce De Leon
D Joseph Jerry
Original Assignee
Univ Massachusetts Public Inst
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Massachusetts Public Inst filed Critical Univ Massachusetts Public Inst
Publication of BR9811657A publication Critical patent/BR9811657A/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/873—Techniques for producing new embryos, e.g. nuclear transfer, manipulation of totipotent cells or production of chimeric embryos
    • C12N15/877—Techniques for producing new mammalian cloned embryos
    • C12N15/8771—Bovine embryos
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K49/00—Preparations for testing in vivo
    • A61K49/0004—Screening or testing of compounds for diagnosis of disorders, assessment of conditions, e.g. renal clearance, gastric emptying, testing for diabetes, allergy, rheuma, pancreas functions
    • A61K49/0008—Screening agents using (non-human) animal models or transgenic animal models or chimeric hosts, e.g. Alzheimer disease animal model, transgenic model for heart failure
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P21/00—Drugs for disorders of the muscular or neuromuscular system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A61P25/14—Drugs for disorders of the nervous system for treating abnormal movements, e.g. chorea, dyskinesia
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A61P25/14—Drugs for disorders of the nervous system for treating abnormal movements, e.g. chorea, dyskinesia
    • A61P25/16—Anti-Parkinson drugs
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A61P25/28—Drugs for disorders of the nervous system for treating neurodegenerative disorders of the central nervous system, e.g. nootropic agents, cognition enhancers, drugs for treating Alzheimer's disease or other forms of dementia
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2517/00—Cells related to new breeds of animals
    • C12N2517/02—Cells from transgenic animals
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2517/00—Cells related to new breeds of animals
    • C12N2517/04—Cells produced using nuclear transfer

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Neurology (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Neurosurgery (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Chemical & Material Sciences (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Developmental Biology & Embryology (AREA)
  • General Engineering & Computer Science (AREA)
  • Psychology (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Diabetes (AREA)
  • Rheumatology (AREA)
  • Toxicology (AREA)
  • Urology & Nephrology (AREA)
  • Epidemiology (AREA)
  • Pathology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Endocrinology (AREA)
  • Psychiatry (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Hospice & Palliative Care (AREA)

Abstract

Patente de Invenção: <B>"CLONAGEM USANDO-SE NúCLEOS DOADORES ORIUNDOS DE CéLULAS DIFERENCIADAS NãO-CARENTES DE SORO''<D>. é provido um método aperfeiçoado de transferência nuclear envolvendo o transplante de núcleos de célula diferenciada doadora oriundos de células não-carentes de soro para dentro de oócitos enucleados da mesma espécie como a célula doadora. As unidades de transferência nuclear resultantes são úteis para a multiplicação de genótipos e genótipos transgênicos pela produção de fetos e de prole, e para a produção de células de CICM isogênicas, incluindo células de tronco ou embriónicas isogênicas humanas. Produção de prole, fetos e embriões de mamífero transgênicos ou geneticamente engenheirados é faciliatado pelo presente método uma vez que a fonte de célula diferenciada dos núcleos doadores pode ser geneticamente modificada e clonalmente propagada.Invention Patent: <B> "CLONING USING NON-LACKING DIFFERENTIATED CELLS FROM SERUM NON-LACKING CELLS '' <D>. An improved method of nuclear transfer involving the transplantation of differentiated donor cell nuclei from non-cell cells is provided. -lack of serum into enucleated oocytes of the same species as the donor cell The resulting nuclear transfer units are useful for the multiplication of transgenic genotypes and genotypes by the production of fetuses and offspring, and for the production of isogenic CICM cells , including human isogenic stem cells or embryonic embryos.Production of transgenic or genetically engineered offspring, fetuses and mammal embryos is facilitated by the present method since the cell source differentiated from the donor nuclei can be genetically modified and clonally propagated.

BR9811657-6A 1997-07-03 1998-06-24 Cloning using donor nuclei from differentiated serum-free cells BR9811657A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US88828397A 1997-07-03 1997-07-03
PCT/US1998/012800 WO1999001163A1 (en) 1997-07-03 1998-06-24 Cloning using donor nuclei from non-serum starved, differentiated cells

Publications (1)

Publication Number Publication Date
BR9811657A true BR9811657A (en) 2000-09-05

Family

ID=25392916

Family Applications (1)

Application Number Title Priority Date Filing Date
BR9811657-6A BR9811657A (en) 1997-07-03 1998-06-24 Cloning using donor nuclei from differentiated serum-free cells

Country Status (9)

Country Link
EP (1) EP1017422A4 (en)
JP (1) JP2001509361A (en)
CN (2) CN1265599A (en)
AU (1) AU8154398A (en)
BR (1) BR9811657A (en)
CA (1) CA2294916A1 (en)
IL (1) IL133786A0 (en)
NZ (1) NZ502129A (en)
WO (1) WO1999001163A1 (en)

Families Citing this family (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6235969B1 (en) * 1997-01-10 2001-05-22 University Of Massachusetts Cloning pigs using donor nuclei from non-quiescent differentiated cells
EP0972017B1 (en) * 1998-01-16 2004-10-06 Agrobiogen GmbH Efficient nuclear transfer using fetal fibroblasts
US6331659B1 (en) 1998-01-21 2001-12-18 University Of Hawaii Cumulus cells as nuclear donors
CA2324009A1 (en) * 1998-03-16 1999-09-23 Relag Pty Ltd. Porcine nuclear transfer
AUPP294898A0 (en) * 1998-04-15 1998-05-07 Monash University Method of nuclear transfer
EP1818397A1 (en) * 1998-11-02 2007-08-15 Trustees Of Tufts College Methods for cloning animals
WO2000025578A2 (en) * 1999-04-26 2000-05-11 Trustees Of Tufts College Methods for cloning animals
US6781030B1 (en) 1998-11-02 2004-08-24 Trustee Of Tufts College, Ballou Hall Methods for cloning mammals using telophase oocytes
EP1375654A3 (en) * 1998-11-02 2008-01-16 GTC Biotherapeutics, Inc. Transgenic and cloned mammals
EP1159415B1 (en) 1999-03-04 2009-12-23 Revivicor, Inc. Genetic modification of somatic cells and uses thereof
JP3652308B2 (en) * 1999-06-30 2005-05-25 ソウル ナショナル ユニバーシティー インダストリー ファウンデーション Clone cattle production method
RU2216591C2 (en) * 1999-06-30 2003-11-20 Воо-Сук ХВАНГ Method for obtaining human cloned embryos due to applying the method of interspecific nuclear transplantation
JP2003503045A (en) * 1999-06-30 2003-01-28 ワン，ウー−サク Clonal tiger production method using xenogeneic nuclear transfer technology
CN1413250A (en) 1999-10-15 2003-04-23 先进细胞技术公司 Method of producing differentiated progenitor cells and lineage-defective embryonic stem cells
DE10084883D2 (en) * 2000-06-08 2004-04-29 Trostner Jens Process for the genetic reconstruction of human organs
EA013564B1 (en) 2000-08-03 2010-06-30 Терапеутик Хьюман Поликлоналз Инк. Humanized immunoglobulin and pharmaceutical composition comprising thereof
FR2814642B1 (en) 2000-10-03 2005-07-01 Ass Pour Le Dev De La Rech En TRANSGENIC MOUSE FOR THE TARGETED RECOMBINATION MEDIATED BY THE MODIFIED CRE-ER
WO2002102997A2 (en) * 2000-11-30 2002-12-27 Stemron Inc. Isolated homozygous stem cells differentiated cells derived therefrom and materials and methods for making and using same
DE60144248D1 (en) * 2000-12-22 2011-04-28 Kyowa Hakko Kirin Co Ltd METHOD FOR THE CLONING OF NON-MENTAL MAMMALS USING REPROGRAMMED DONORCHROMATIN OR DONORCELLS
EP1217071B1 (en) 2000-12-22 2010-04-28 Institut National De La Recherche Agronomique Position-independent and tissue specific expression of a transgene in milk of transgenic animals
CN1568366B (en) 2001-08-13 2013-04-24 恩布里克斯公司 Methods for injecting avian eggs
NZ535367A (en) * 2002-04-01 2008-01-31 Gtc Biotherapeutics Inc A method for selecting cell lines to be used for nuclear transfer in mammalian species
US7612250B2 (en) 2002-07-29 2009-11-03 Trustees Of Tufts College Nuclear transfer embryo formation method
CN101506235B (en) 2006-09-01 2012-07-25 人类多细胞株治疗学公司 Enhanced expression of human or humanized immunoglobulin in non-human transgenic animals
EP2267153A1 (en) 2009-05-26 2010-12-29 Université Claude Bernard Lyon 1 Identification of netrin-1 receptor unc5c gene mutation in solid cancers
US10865383B2 (en) 2011-07-12 2020-12-15 Lineage Cell Therapeutics, Inc. Methods and formulations for orthopedic cell therapy

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0739412B1 (en) * 1993-12-23 2002-02-27 Infigen, Inc. ungulate EMBRYONIC STEM CELLS AS NUCLEAR DONORS AND NUCLEAR TRANSFER TECHNIQUES TO PRODUCE CHIMERIC AND TRANSGENIC ANIMALS
GB9517780D0 (en) * 1995-08-31 1995-11-01 Roslin Inst Edinburgh Biological manipulation
GB9517779D0 (en) 1995-08-31 1995-11-01 Roslin Inst Edinburgh Biological manipulation
US5945577A (en) 1997-01-10 1999-08-31 University Of Massachusetts As Represented By Its Amherst Campus Cloning using donor nuclei from proliferating somatic cells
EP0972017B1 (en) * 1998-01-16 2004-10-06 Agrobiogen GmbH Efficient nuclear transfer using fetal fibroblasts

Also Published As

Publication number Publication date
JP2001509361A (en) 2001-07-24
IL133786A0 (en) 2001-04-30
CA2294916A1 (en) 1999-01-14
NZ502129A (en) 2005-02-25
AU8154398A (en) 1999-01-25
EP1017422A4 (en) 2002-10-29
EP1017422A1 (en) 2000-07-12
WO1999001163A1 (en) 1999-01-14
CN101003800A (en) 2007-07-25
CN1265599A (en) 2000-09-06

Similar Documents

Publication Publication Date Title
BR9811657A (en) Cloning using donor nuclei from differentiated serum-free cells
BR9811659A (en) Cloning of pigs using differentiated cell donor nuclei
BR9806872A (en) Nuclear transfer with differentiated fetal and adult donor cells
Kaneda et al. Genetic evidence for Dnmt3a‐dependent imprinting during oocyte growth obtained by conditional knockout with Zp3‐Cre and complete exclusion of Dnmt3b by chimera formation
ATE318307T1 (en) CULTURE OF A CELL LINE FROM THE INNER CELL MASS OF UNGARDEN EMBRYOS.
DK1044262T3 (en) Gender-specific insemination of mammals with a low number of sperm
BR9913643A (en) Transgenesis of mammals through intracytoplasmic sperm injection
BR0209028A (en) Nuclear transfer method
Arat et al. Using cell banks as a tool in conservation programmes of native domestic breeds: the production of the first cloned Anatolian Grey cattle
Han et al. Overexpression of Tet3 in donor cells enhances goat somatic cell nuclear transfer efficiency
IL146296A0 (en) Cell reprogramming
WO2000026357A3 (en) Transgenic and cloned mammals
Reyes Palomares et al. Update on the epigenomic implication of embryo cryopreservation methods applied in assisted reproductive technologies with potential long-term health effects
BR0013959A (en) Cloning of pigs using donor cells or nuclei from differentiated cells and production of pluripotent porcine
Honda Applying iPSCs for preserving endangered species and elucidating the evolution of mammalian sex determination
EP1375654A3 (en) Transgenic and cloned mammals
WO2001060152A3 (en) Transgenic non-human ucp2 knockout mammal
BR0010588A (en) Nuclear transfer telophase enucleated oocytes
Shimozawa et al. Direct production of gene-targeted mice from ES cells by nuclear transfer and gene transmission to their progeny
EP1405068A4 (en) METHODS FOR CLONING MAMMALS WITH REMODELING FACTORS
DE69940057D1 (en) SOURCE FOR CELL CHERRIES FOR CORE TRANSFER
Sansinena et al. 52 Initiation of pregnancies in south african riverine rabbit (bunolagus monticulares) by interspecies nuclear transfer using adipose-derived somatic cells
Cocquet et al. The Multicopy Gene Sly Represses the Sex Chromosomes in the Male Mouse
CA2431859A1 (en) Transgenic and cloned mammals
Farag The role of DNA-methyltransferase 3-like (DNMT3L) in the establishment and stability of DNA Methylation patterns in the male germline

Legal Events

Date Code Title Description
B07A Application suspended after technical examination (opinion) [chapter 7.1 patent gazette]
B07A Application suspended after technical examination (opinion) [chapter 7.1 patent gazette]
B09B Patent application refused [chapter 9.2 patent gazette]

Free format text: DE ACORDO COM O ART. 36 DA LPI (LEI 9279/96) 2O, O PEDIDO VOLTA A EXAME SENDO CONSIDERADO NAO PATENTEAVEL PELAS RAZOES EXPOSTAS NO PARECER TECNICO ANTERIOR. PORTANTO, INDEFIRO O PRESENTE PEDIDO DE PATENTE COMO INVENCAO - DE ACORDO COM O ART. 8O DA LPI.

B09B Patent application refused [chapter 9.2 patent gazette]

Free format text: MANTIDO O INDEFERIMENTO UMA VEZ QUE NAO FOI APRESENTADO RECURSO DENTRO DO PRAZO LEGAL.