Научно-Изследов. Произв. Ветеринарно-Медицински Институт По Имунология
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Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents
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Abstract
The vaccine is inactivated by ethanol and is harmless. Theantigene for its preparation is produced after cultivation underroller conditions of the local cellular cultural strain DD/94 ofcells of the permanent line VIC21. The viral suspension producedwith infection titre 107.5 KID50 is adsorbed in 2% gel ofaliminium hydroxide and is settling for 48 h, after which thesupernatant is removed to 50% of the initial volume and isinactivated by ethanol. As an oleoadjuvant to the antigene mineraloil emulsified by montanide 888 is added in 3:2 ratio. Theimmunogeneity of the vaccine is tested on 18-20-week chicksimmunized with live vaccine in advance. On the 25th day after thevaccination a group of 10 chicks are infested by a virulent strainof the fowl pest virus. Simultaneoulsy a control group of 10unvaccined chicks is also infested. For 15 days 90% of thevaccined chicks remain in good health, and 90% of the controls diewith symptoms of fowl pest.2 claims.
BG100932A1996-10-221996-10-22Method for the preparation of cellular cultural inactivated vaccine against fowl pest
BG100932A
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