AU717432B2 - Methods of using (+) cisapride for the treatment of gastro-esophageal reflux disease and other disorders - Google Patents

Description

AUSTRALIA

Patents Act COMPLETE SPECIFICATION

(ORIGINAL)

Class Int. Class Application Number: Lodged: Complete Specification Lodged: Accepted: Published: Priority Related Art: Name of Applicant: Sepracor, Inc.

Actual Inventor(s): Nancy M. Gray James W. Young Address for Service: PHILLIPS ORMONDE FITZPATRICK Patent and Trade Mark Attorneys 367 Collins Street Melbourne 3000 AUSTRALIA Invention Title: METHODS OF USING CISAPRIDE FOR THE TREATMENT OF GASTRO-ESOPHAGEAL REFLUX DISEASE AND OTHER DISORDERS Our Ref 512520 POF Code: 1443/70025 The following statement is a full description of this invention, including the best method of performing it known to applicant(s): -1- -lae g.

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0 METHODS OF USING CISAPRIDE FOR THE TREATMENT OF GASTRO-ESOPHAGEAL REFLUX DISEASE AND OTHER DISORDERS The present application is a divisional application from Australian patent application number 47705/93, the entire disclosure of which is incorporated herein by reference.

1. TECHNICAL FIELD This invention relates to novel compositions of matter containing optically pure cisapride. These compositions possess potent activity in treating emesis while avoiding the adverse effects associated with the administration of the racemic mixture of cisapride. Also disclosed are methods for treating the above-described condition in a human while avoiding the adverse effects that are associated with the racemic mixture of cisapride, by administering the isomer of cisapride to a human in need of such treatment.

The active compound of these compositions and methods is an optical isomer of racemic cis-cisapride, which is described in European Patent Application No. 0,076,530 A2 published April 13, 1983 and United States Patent Nos. 4,962,115 and 5,057,525. Chemically, the active compound, of the presently disclosed compositions and methods, is the isomer of cis-4-amino- 5-chloro-N-[1-[3-(4-fluorophenoxy)propyl]-3-methoxy-4-piperidinyl]-2methoxybenzamide, hereinafter referred to as cisapride. The term C \winwordnnaVNB\NODELETE\Specifications4707dc 2 isomer of cisapride" and particularly the term cisapride" encompass optically pure and substantially optically pure cisapride.

Similarly, as used herein, the terms "racemic cisapride" or "racemic mixture of cisapride" refer to the cis diastereomeric racemate.

Cisapride, which is the subject of the present invention, is available commercially only as the 1:1 racemic mixture of the cis diastereomeric racemate.

Cisapride is available only as a mixture of optical isomers, called enantiomers, a mixture of cis(+) and cis(-) cisapride.

2. BACKGROUND OF THE INVENTION 2.1 Steric Relationship and Drug Action Many organic compounds exist in optically active forms, i.e. they have the ability to rotate the plane of plane polarized light. In describing an optically 20 active compound, the prefixes D and L or R and S are used to denote the absolute configuration of the molecule about its chiral center(s). The prefixes d and 1 or and are employed to designate the sign of rotation of plane-polarized light by the 25 compound, with or 1 meaning that the compound is levorotatory. A compound prefixed with or d is dextrorotatory. For a given chemical structure, these compounds, called stereoisomers, are identical except that they are mirror images of one another. A specific stereoisomer may also be referred to as an S" enantiomer, and a mixture of such isomers is often called an enantiomeric or racemic mixture.

Stereochemical purity is of importance in the field of pharmaceuticals, where 12 of the 20 most prescribed drugs exhibit chirality. A case in point is provided by the L-form of the beta-adrenergic 3 blocking agent, propranolol, which is known to be 100 times more potent than the D-enantiomer.

Furthermore, optical purity is important since certain isomers may actually be deleterious rather than simply inert. For example, it has been suggested that the D-enantiomer of thalidomide was a safe and effective sedative when prescribed for the control of morning sickness during pregnancy, while the corresponding L-enantiomer has been believed to be a potent teratogen.

2.2 Pharmacologic Action United States Patent Nos. 4,962,115 and 5,057,525, issued to Georges Van Daele, disclose N-(3hydroxy-4-piperidenyl)benzamides including the cis and trans diastereomeric racemates of cisapride. Van ".Daele discloses that these compounds, the pharmaceutically acceptable acid addition salts 20 thereof and the stereochemically isomeric forms thereof, stimulate the motility of the gastrointestinal system. Van Daele states that the diastereomeric racemates of these compounds may be obtained separately by conventional methods and that these diastereomeric racemates may be further resolved into their optical isomers. Van Daele also reports the "lowest effective concentration... whereby a significant stimulation of the acetylcholine release is noted", for cis(+) and cis(-) cisapride, to be 0.01 30 mg/L and 0.04 mg/L respectively while the "lowest effective dose whereby antagonistic effects of dopamine-induced gastric relaxation are observed" is reported to be 0.63 mg/L for both cis(+) and cis(-) cisapride. Therefore, Van Daele teaches that cis(+) and cis(-) cisapride have identical pharmacological profiles.

4 Cisapride is one of a class of compounds known as benzamide derivatives, the parent compound of which is metoclopramide (See: Schapira et al., Acta Gastro- Enterolog. Belg. LIII: 446-457, 1990). As a class, these benzamide derivatives have several prominent pharmacological actions. The prominent pharmacological activities of the benzamide derivatives are due to their effects on the neuronal systems which are modulated by the neurotransmitter serotonin. The role of serotonin, and thus the pharmacology of the benzamide derivatives, has been broadly implicated in a variety of conditions for many years (See Phillis, "The Pharmacology of Synapses", Pergamon Press, Monograph 43, 1970; Frazer, A. et al., Annual Rev. of Pharmacology and Therapeutics 30: 307-348, 1990). Thus, research has focused on locating the production and storage sites of serotonin as well as the location of serotonin 20 receptors in the human body in order to determine the connection between these sites and various disease states or conditions.

In this regard, it was discovered that a major site of production and storage of serotonin is the e 25 enterochromaffin cell of the gastrointestinal mucosa.

It was also discovered that serotonin has a powerful Sstimulating action on intestinal motility by stimulating intestinal smooth muscle, speeding intestinal transit, and decreasing absorption time, as 30 in diarrhea. This stimulating action is also associated with nausea and vomiting.

Because of their modulation of the serotonin neuronal system in the gastrointestinal tract, many of the benzamide derivatives are effective antiemetic agents and are commonly used to control vomiting during cancer chemotherapy or radiotherapy, especially when highly emetogenic compounds such as cisplatin are used (See: Costall et al., Neuropharmacology 26: 1321-1326, 1987). This action is almost certainly the result of the ability of the compounds to block the actions of serotonin (5HT) at specific sites of action, called the 5HT3-receptor, which was classically designated in the scientific literature as the serotonin M-receptor (See: Clarke et al., Trends in Pharmacological Sciences 10: 385-386, 1989). Chemo- and radiotherapy may induce nausea and vomiting by the release of serotonin from damaged enterochromaffin cells in the gastrointestinal tract. Release of the neurotransmitter serotonin stimulates both afferent vagal nerve fibers (thus initiating the vomiting reflex) and serotonin receptors in the chemoreceptor trigger zone of the area postrema region of the brain. The anatomical site for this action of the benzamide derivatives, especially whether it is central(CNS), peripheral, or a combination thereof, remains unresolved (See: Barnes et al., J. Pharm.

Pharmacol. 40: 586-588, 1988). Cisapride, being a benzamide derivative may have been expected to be an effective antiemetic agent based on the benzamide's believed ability to modulate the activity of serotonin at the 5HT3 receptor. However, as reported in Schapira et. al. (referred to above) it was previously believed that cisapride exhibited no antiemetic activity.

A second prominent action of the benzamide derivatives is in augmenting gastrointestinal smooth muscle activity from the esophagus through the proximal small bowel, thus accelerating esophageal and small intestinal transit as well as facilitating gastric emptying and increasing lower esophageal sphincter tone (See: Decktor et al., Eur. J. Pharmacol. 147: 313-316, 1988). Although the benzamide derivatives are not cholinergic receptor agonists per se, the aforementioned smooth muscle effects may be blocked by muscarinic receptor blocking 9* C:\WINORD\JENNIFER\MNBNODELETE\P47705.DOC 6 agents such as atropine or neuronal transmission inhibitors of the tetrodotoxin type which affect sodium channels (See: Fernandez and Massingham, Life Sci. 36: 1-14, 1985). Similar blocking activity has been reported for the contractile effects of serotonin in the small intestine (See: Craig and Clarke, Brit.

J. Pharmacol. 96: 247P, 1989). It is currently believed that the primary smooth muscle effects of the benzamide derivatives are the result of an agonist action upon a new class of serotonin receptors referred to as 5HT4 receptors which are located on interneurons in the myenteric plexus of the gut wall (See Clarke et al., Trends in Pharmacological Sciences 10: 385-386, 1989 and Dumuis et al., N. S. Arch.

Pharmacol. 340: 403-410, 1989). Activation of these receptors subsequently enhances the release of acetylcholine from parasympathetic nerve terminals located near surrounding smooth muscle fibers and it 20 is the combination of acetylcholine with its receptors on smooth muscle membranes which is the actual trigger for muscle contraction.

Racemic cisapride possesses similar properties to metoclopramide except that it lacks dopamine receptor 25 blocking activity (See: Reyntjens et al., Curr.

Therap. Res. 36: 1045-1046, 1984) and enhances motility in the colon as well as in the upper portions of the alimentary tract (See: Milo, Curr. Therap. Res.

36: 1053-1062, 1984). The colonic effects, however, may not be completely blocked by atropine and may represent, at least in part, a direct action of the drug (See: Schuurkes et al., J. Pharmacol Exp. Ther.

234: 775-783, 1985). Using cultured mouse embryo colliculi neurons and cAMP generation as an endpoint for designating 5HT4 activity, the EC50 concentration of racemic cisapride was 7 X 10- 8 M (See: Dumuis et 7 al., N. S. Arch. Pharmacol. 340: 403-410, 1989).

Drugs of this class do not affect gastric acid secretion and have variable effects upon colonic motility (See: Reyntjens et al., Curr. Therap. Res.

36: 1045-1046, 1984 and Milo, Curr. Therap. Res. 36: 1053-1062, 1984).

The racemic mixture of cisapride is presently used primarily to treat gastro-esophageal reflux disease. This disease is characterized as the backward flow of the stomach contents into the esophagus. One of the most important factors in the pathogenesis of gastro-esophageal reflux disease is a reduction in the pressure barrier due to the failure of the lower esophageal sphincter. Failure of the lower esophageal sphincter can arise due to a low basal pressure, sphincter relaxation, or to a noncompensated increase in intragastric pressure. Other factors in the pathogenesis of the disease are delayed 20 gastric emptying, insufficient esophageal clearing due to impaired peristalsis and the corrosive nature of the reflux material which can damage esophageal mucosa. The racemic mixture of cisapride is thought to strengthen the anti-reflux barrier and improve 25 esophageal clearance by increasing the lower esophageal sphincter pressure and enhancing peristaltic contractions.

S* Because of its activity as a prokinetic agent, the racemic mixture of cisapride may also be useful to 30 treat dyspepsia, gastroparesis, constipation, postoperative ileus, and intestinal pseudo-obstruction.

Dyspepsia is a condition characterized by an impairment of the power or function of digestion that can arise as a symptom of a primary gastrointestinal dysfunction or as a complication due to other disorders such as appendicitis, gallbladder 8 disturbances, or malnutrition. Gastroparesis is a paralysis of the stomach brought about by a motor abnormality in the stomach or as a complication of diseases such as diabetes, progressive systemic sclerosis, anorexia nervosa and myotonic dystrophy.

Constipation is a condition characterized by infrequent or difficult evacuation of feces resulting from conditions such as lack of intestinal muscle tone or intestinal spasticity. Post-operative ileus is an obstruction in the intestine due to a disruption in muscle tone following surgery. Intestinal pseudoobstruction is a condition characterized by constipation, colicky pain, and vomiting, but without evidence of physical obstruction.

Furthermore, some of the benzamide derivatives have activity as dopamine D2 receptor antagonists and as such, may induce adverse (side) effects such as extrapyramidal tract symptoms and hyperprolactinemia 20 characteristic of this drug class (See: Costall et al., Neuropharmacology 26: 1321-1326, 1987). These adverse effects are not, however, representative of all the compounds in the benzamide class: they are not shared, for example, by racemic cisapride (See: Reyntjens et al., Curr. Therap. Res. 36: 1045-1046, 1984). However, the administration of the racemic mixture of cisapride to a human has been found to cause other adverse effects. These adverse effects include, but are not limited to diarrhea and abdominal cramping. Further, it has been reported that intravenous administration of racemic cisapride demonstrates the occurrence of additional adverse (side) effects not experienced after oral administration of racemic cisapride. Stacher et al.

Digestive Diseases and Sciences 32(11): 1223-1230 (1987).

-9- Racemic cisapride is almost completely absorbed after oral administration to humans but the bioavailability of the parent compound is only 40-50%, due to rapid first pass metabolism in the liver (See: Van Peer et al., in Progress in the Treatment of Gastrointestinal Motility Disorders: The Role of Cisapride. Proceedings of a Symposium in Frankfurt. November 1986.

Johnson A. G. and Lux, G. eds. Excerpta Medica, Amsterdam, pp. 23-29 (1988). More than 90% of a dose of cisapride is metabolised mainly by oxidative N-dealkylation at the piperidine nitrogen or by aromatic hydroxylation occurring on either the 4-fluorophenoxy or benzamide rings. Poor bioavailability, as seen with racemic cisapride, can often be associated with erratic pharmacological effects following oral administration of compounds.

The above discussion of documents, acts, materials, devices, articles :.and the like is included in this specification solely for the purpose of providing a 15 context for the present invention. It is not suggested or represented that any or all of these matters formed part of the prior art base or were common general knowledge in the field relevant to the present invention as it existed in Australia before the priority date of each claim of this application.

3. SUMMARY OF THE INVENTION 0 25 It has now been discovered that optically pure cisapride is an effective antiemetic agent, useful as an adjunctive therapy in cancer treatment to alleviate nausea and vomiting induced by chemo- or radio-therapeutics, while avoiding the above-described adverse effects associated with the administration of the racemic mixture of cisapride.

The present invention includes methods for treating the above-described condition in a human while avoiding the adverse effects that are associated with the racemic mixture of cisapride, by administering the optically pure isomer of cisapride to said human. Furthermore, the present invention also includes methods and compositions which demonstrate an improved bioavailability over racemic cisapride irrespective of the mode of administration.

C: onwrd\AnnaNB\NODELETE Specificatn677-97.do 4. DETAILED DESCRIPTION OF THE INVENTION The present invention encompasses a method of eliciting an antiemetic effect in a human, while avoiding the concomitant liability of adverse effects associated with the administration of racemic cisapride, which comprises administering to a human in need of such antiemetic therapy, an amount of cisapride, or a pharmaceutically acceptable salt thereof, substantially free of its stereoisomer, said amount being sufficient to alleviate nausea and vomiting but insufficient to cause adverse effects associated with the administration of racemic cisapride.

0 In addition, the present invention encompasses an antiemetic composition for the treatment of a human in need of antiemetic therapy, which comprises an amount of cisapride, or a pharmaceutically acceptable salt 15 thereof, substantially free of its stereoisomer, said amount being sufficient to alleviate nausea and vomiting but insufficient to cause adverse effects associated with the administration of racemic cisapride.

0000 Therefore, the present invention also encompasses a method of eliciting 20 an antiemetic effect in a human while achieving a higher bioavailability than racemic cisapride which comprises administering to a human in need of antiemetic therapy, an amount of cisapride, or a pharmaceutically acceptable salt thereof, substantially free of its stereoisomer, said amount being sufficient to alleviate nausea and vomiting and said amount having higher 25 bioavailability than racemic cisapride.

In addition, the present invention encompasses methods for treating the above-described condition which comprises administering to a human in need of treatment from such a condition, an amount of cisapride, or a pharmaceutically acceptable salt thereof, substantially free of its stereoisomer, said amount having increased bioavailability over the racemic mixture and being sufficient to alleviate said condition but being insufficient to cause adverse effects associated with racemic cisapride.

C:winwo'AnnaWNBNODELETESpecifcations%6770-97doc -11 The available racemic mixture of cisapride a 1:1 racemic mixture of the two cis enantiomers) possesses prokinetic and antiemetic activity, and provides therapy and a reduction of symptoms in a variety of conditions and disorders related to gastrointestinal motility dysfunction; however, this racemic mixture, while offering the expectation of efficacy, causes adverse effects and has a relatively low bioavailability level. Utilizing the substantially optically pure or optically pure isomer of cisapride results in clearer dose related definitions of efficacy, diminished adverse effects, and accordingly, an improved therapeutic index as well as as a higher bioavailability. It is therefore, more desirable to use the isomer of cisapride.

The term "adverse effects" includes, but is not limited to gastro-intestinal disorders such as diarrhea, abdominal cramping and abdominal grumbling, 5. tiredness, headache, increased systolic pressure, increased heart rate, neurological and CNS disorders and interaction of cisapride with other drugs given concomitantly such as digoxin, diazepam, ethanol, acenocoumarol, cimetidine, ranitidine, paracetamol, and propranolol.

The terms "substantially free of its stereoisomer", "substantially 00* 20 optically pure isomer of cisapride", "substantially optically pure cisapride", S "optically pure isomer of cisapride" and "optically pure cisapride" as used herein mean that the compositions contain a greater proportion of the isomer of cisapride in relation to the isomer of cisapride. In a preferred q* embodiment, the composition contains at least 90% by weight of cisapride 25 and 10% by weight or less of cisapride. In a more preferred embodiment the composition contains at least 99% by weight of cisapride, and 1% or less of cisapride. In a most preferred embodiment, the term "substantially free of its stereoisomer" as used herein means that the composition contains greater than 99% by weight of cisapride. These percentages are based upon the total amount of cisapride in the composition.

The term "gastro-esophageal reflux disease" as used herein means preventing the incidence of, and C:\winword\nn \MN BVIOELETESpclirations4709do -12treating the symptoms of those conditions causing the backward flow of the stomach contents into the esophagus.

The term "eliciting an antiemetic effect" as used herein means providing relief from or preventing the symptoms of nausea and vomiting induced spontaneously or associated with emetogenic cancer chemotherapy or irradiation therapy. Similarly, "antiemetic therapy" as used herein means providing relief from or .prevention of the symptoms of nausea and vomiting induced spontaneously or associated with emetogenic ""cancer chemotherapy or irradiation therapy.

The term "treating a condition caused by 15 gastrointestinal motility dysfunction" as used herein .;O0 means treating the symptoms and conditions associated with this disorder which include, but are not limited to dyspepsia, gastroparesis, constipation, postoperative ileus, and intestinal pseudo-obstruction.

20 The term "prokinetic" as used herein means the enhancement of peristalsis in, and thus the movement through the gastrointestinal tract.

The term "dyspepsia" as used herein means a condition characterized by an impairment of the power or function of digestion that can arise as a symptom of a primary gastrointestinal dysfunction or as a S. complication due to other disorders such as

SS

appendicitis, gallbladder disturbances, or malnutrition.

The term "gastroparesis" as used herein means a paralysis of the stomach brought about by a motor abnormality in the stomach or as a complication of diseases such as diabetes, progressive systemic sclerosis, anorexia nervosa and myotonic dystrophy.

The term "constipation" as used herein means a condition characterized by infrequent or difficult -13evacuation of feces resulting from conditions such as lack of intestinal muscle tone or intestinal spasticity.

The term "post-operative ileus" as used herein means an obstruction in the intestine due to a disruption in muscle tone following surgery.

The term "intestinal pseudo-obstruction" as used herein means a condition characterized by constipation, colicky pain, and vomiting, but without evidence of physical obstruction.

The chemical synthesis of the racemic mixture of cisapride can be performed by the method described in European Patent Application No. 0,076,530 A2 published 15 April 13, 1983, U.S. Patent Nos. 4,962,115 and 5,057,525 and in Van Daele et al., Drug Development Res. 8: 225-232 (1986), each of which is hereby incorporated by reference. The isomer of cisapride may be obtained from its racemic mixture by 20 resolution of the enantiomers using conventional means such as from an optically active resolving acid. See, for example, "Enantiomers, Racemates and Resolutions," by J. Jacques, A. Collet, and S.H. Wilen, (Wiley- Intenscience, New York, 1981); S.H. Wilen, A. Collet, and J. Jacques, Tetrahedron, 33, 2725 (1977); and "Stereochemistry of Carbon Compounds, by E.L. Eliel (McGraw-Hill, NY, 1962) and S.H. Wilen, page 268, in "Tables of Resolving Agents and Optical Resolutions" Eliel, Ed. Univ. of Notre Dame Press, Notre Dame, IN, 1972). Furthermore, the optically pure isomer of cisapride can be prepared from the racemic mixture by enzymatic biocatalytic resolution. See, for example, United States Patent Nos. 5,057,427 and 5,077,217, the disclosures of which are incorporated by reference.

-14- The magnitude of a prophylactic or therapeutic dose of cisapride in the acute or chronic management of diseases and/or disorders described herein will vary with the severity of the condition to be treated, and the route of administration. The dose, and perhaps the dose frequency, will also vary according to the age, body weight, and response of the individual patient. In general, the total daily dose range for cisapride, for the conditions described @see herein, is from about 1 mg to about 200 mg, in single or divided doses. Preferably, a daily dose range .should be between about 5 mg to about 100 mg, in Os osingle or divided doses, while most preferably, a 15 daily dose range should be between about 5 mg to about 75 mg, in single or divided doses. It is preferred 0000 that the doses are administered from 1 to 4 times a day and most preferably 3 to 4 times a day. In managing the patient, the therapy should be initiated 20 at a lower dose, perhaps about 5 mg to about 10 mg, o- *and increased up to about 50 mg or higher depending on the patient's global response. It is further 0.:recommended that children, and patients over 65 years, and those with impaired renal or hepatic function, initially receive low doses, and that they be titrated o ~based on individual response(s) and blood level(s).

oo•0 It may be necessary to use dosages outside these 6S ranges in some cases as will be apparent to those skilled in the art. Further, it is notedthat the clinician or treating physician would know how and when to interrupt, adjust, or terminate therapy in conjunction with individual patient response. The terms "an amount sufficient to alleviate said reflux disease, but insufficient to cause the adverse effects", "an amount sufficient to alleviate nausea and vomiting but insufficient to cause adverse effects", and "an amount sufficient to alleviate said condition caused by gastrointestinal motility dysfunction, but insufficient to cause adverse effects" are encompassed by the above-described dosage amounts and dose frequency schedule.

Any suitable route of administration may be employed for providing the patient with an effective dosage of cisapride. For example, oral, rectal, parenteral (subcutaneous, intramuscular, intravenous), transdermal, and like forms of administration may be employed. Dosage forms include tablets, troches, dispersions, suspensions, solutions, capsules, patches, and the like.

is 15 The pharmaceutical compositions of the present invention comprise cisapride as the active ingredient, or a pharmaceutically acceptable salt thereof, and may also contain a pharmaceutically acceptable carrier, and optionally, other therapeutic 20 ingredients The term "pharmaceutically acceptable salts" or "a pharmaceutically acceptable salt thereof" refer to salts prepared from pharmaceutically acceptable nontoxic acids or bases including inorganic acids and bases and organic acids and bases. Since the compound of the present invention is basic, salts may be prepared from pharmaceutically acceptable non-toxic acids including inorganic and organic acids. Suitable pharmaceutically acceptable acid addition salts for the compound of the present invention include acetic, benzenesulfonic (besylate), benzoic, camphorsulfonic, citric, ethenesulfonic, fumaric, gluconic, glutamic, hydrobromic, hydrochloric, isethionic, lactic, maleic, malic, mandelic, methanesulfonic, mucic, nitric, pamoic, pantothenic, phosphoric, succinic, sulfuric, tartaric acid, p-toluenesulfonic, and the like.

-16- Preferred acid addition salts are the chloride and sulfate salts. In the most preferred embodiment, cisapride is administered as the free base.

The compositions of the present invention include compositions such as suspensions, solutions and elixirs; 'aerosols; or carriers such as starches, sugars, microcrystalline cellulose, diluents, granulating agents, lubricants, binders, disintegrating agents, and the like, in the case of 00.0 oral solid preparations (such as powders, capsules, and tablets) with the oral solid preparations being preferred over the oral liquid preparations. A preferred oral solid preparation is capsules. The 15 most preferred oral solid preparation is tablets.

Because of their ease of administration, tablets and capsules represent the most advantageous oral dosage unit form, in which case solid pharmaceutical carriers are employed. If desired, tablets may be We** 20 coated by standard aqueous or nonaqueous techniques.

In addition to the common dosage forms set out above, the compounds of the present invention may also be administered by controlled release means and/or delivery devices such as those described in U.S.

25 Patent Nos.: 3,845,770; 3,916,899; 3,536,809; [3,598,123; and 4,008,719, the disclosures of which are hereby incorporated by reference.

go Pharmaceutical compositions of the present invention suitable for oral administration may be presented as discrete units such as capsules, cachets, or tablets, or aerosols sprays, each containing a predetermined amount of the active ingredient, as a powder or granules, or as a solution or a suspension in an aqueous liquid, a non-aqueous liquid, an oil-inwater emulsion, or a water-in-oil liquid emulsion.

Such compositions may be prepared by any of the -17methods of pharmacy, but all methods include the step of bringing into association the active ingredient with the carrier which constitutes one or more necessary ingredients. In general, the compositions are prepared by uniformly and intimately admixing the active ingredient with liquid carriers or finely divided solid carriers or both, and then, if necessary, shaping the product into the desired presentation.

For example, a tablet may be prepared by compression or molding, optionally, with one or more -accessory ingredients. Compressed tablets may be prepared by compressing in a suitable machine the 15 active ingredient in a free-flowing form such as powder or granules, optionally mixed with a binder, qe..

lubricant, inert diluent, surface active or dispersing agent. Molded tablets may be made by molding in a suitable machine, a mixture of the powdered compound OeDO 20 moistened with an inert liquid diluent. Desirably, each tablet contains from about 1 mg to about 100 mg of the active ingredient, and each cachet or capsule :contains from about 1 mg to about 50 mg of the active ingredient. Most preferably, the tablet, cachet or capsule contains either one of three dosages, about ~mg, about 10 mg and about 25 mg of the active .o ingredient.

The invention is further defined by reference to the following examples describing in detail, the preparation of the compound, and the compositions of the present invention. It will be apparent to those skilled in the art, that many modifications, both to materials, and methods, may be practiced without departing from the purpose and interest of this invention.

-18-

EXAMPLES

5.1 EXAMPLE 1 Determination of Oral Bioavailability The relative oral bioavailability of optically pure cisapride and racemic cisapride is determined by a pharmacological study in dogs. Evaluation of these compounds is based on their relative potencies in a test to measure gastric emptying as an index of prokinetic activity in the stomach. Briefly, the compounds are dissolved or suspended in methylcellulose and administered at varying doses via S' an indwelling gastric fistula to adult beagle dogs.

The compounds are given 60 minutes prior to the 15 administration via fistula of a liquid test meal containing the dye phenol red. The gastric contents are collected 5 minutes later via gravity drainage through the fistula. Gastric emptying during this period may be calculated according to the formula of 20 Debas (See: Fitzpatrick et al., J. Pharmacol. Exp.

*0 Ther. 254: 450-455, 1990) which takes into account both the volume of meal collected and the concentration of the dye. Relative potencies between cisapride, cisapride and racemic cisapride are 25 assessed via standard parallel line assays.

5 5.2 EXAMPLE 2 5HT3 Receptor binding The affinity of compounds for the 5HT3 receptor is assessed via a radioligand binding assay using membranes derived from the cerebral cortex of rat brains (See: Fitzpatrick et al., J. Pharmacol. Exp.

Ther. 254: 450455, 1990). Essentially, plasma membranes from this animal source are equilibrated in test tubes with solutions containing the radioactive 5HT3 receptor ligand quipazine and various -19concentrations of cisapride, cisapride and racemic cisapride. After incubation for 30 minutes, the membranes are isolated on a filter and the degree of inhibition of quipazine binding determined. Based on the results obtained, the concentration of each compound which inhibits quipazine binding by 50% (the are calculated.

5.3 EXAMPLE 3 5HT4 Receptor Aqonist Activity oo Agonist activity at 5HT4 receptor sites are S* assessed using an assay based on the ability of active compounds to increase cyclic AMP production in mouse 15 embryo colloculi neurones grown in tissue culture (See: Dumuis et al., N. S. Arch. Pharmacol. 340: 403- 410, 1989). Basically, cisapride, cisapride and racemic cisapride, at varying concentrations, are incubated with these cells for 10 minutes in the 20 presence of the cAMP precursor substance, ATP. At the end of this period, the degree of formation of cAMP is assessed. The concentration of agonist compound required to increase the formation of cAMP by 50% of the maximal possible (the EC50) is then calculated.

5.4 EXAMPLE 4 Determination of Cardiovascular Effects Unanesthetized spontaneously hypertensive rats (SHR) with systolic blood pressures ranging between 180 and 220 mm Hg are used. Blood pressure is recorded indirectly in a temperature-controlled environment before, 1, 2, and 4 hours after the test substance is administered p.o. The test substances are racemic, and cisapride. Increases in systolic blood pressure by more than 10% at any two of the aforementioned three consecutive time points is considered significant. In the same spontaneously hypertensive rats, heart rate is recorded by a cardiograph immediately after the blood pressure recordings. An increase in heart rate greater than 20 percent from pre-treatment control readings is considered significant.

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Tablets Formula Quantity per Tablet in mg A B C Active Ingredient cisapride 5.0 10.0 25.0 Lactose BP 62.0 57.0 42.0 Starch BP 20.0 20.0 20.0 Microcrystalline Cellulose 10.0 10.0 10.0 Hydrogenated Vegetable Oil 1.5 1.5 Polyvinylpyrrolidinone 1.5 1.5 Compression Weight 100.0 100.0 100.0 5 The active ingredient, cisapride, is sieved through a suitable sieve and blended with the lactose until a uniform blend is formed. Suitable volumes of water are added and the powders are granulated. After drying, the granules are then screened and blended with the remaining excipients. The resulting granules are then compressed into tablets of desired shape. Tablets of other 10 strengths may be prepared by altering the ratio of active ingredient to the excipient(s) or the compression weight.

Throughout the description and claims of this specification, the word "comprise" and variations of the word, such as "comprising" and "comprises", is 15 not intended to exclude other additives or components or integers.

C:\winword\AnnaWNB\NODELETE\Specifications\46770-97.doc

Claims (17)

1. A method of eliciting an antiemetic effect in a human while avoiding the concomitant liability of adverse effects associated with racemic cisapride, which comprises administering to a human in need of antiemetic therapy, an amount of cisapride, or a pharmaceutically acceptable salt thereof, substantially free of its stereoisomer, said amount being sufficient to alleviate nausea and vomiting but insufficient to cause said adverse effects.

2. A method of eliciting an antiemetic effect in a human while achieving a higher bioavailability than racemic cisapride which comprises administering to a human in need of antiemetic therapy, an amount of cisapride, or a °0 pharmaceutically acceptable salt thereof, substantially free of its stereoisomer, said amount being sufficient to alleviate nausea and vomiting S" 15 while having a higher bioavailability than racemic cisapride.

3. A method according to either claim 1 or 2 wherein cisapride is administered by intravenous infusion, transdermal delivery, or orally as a tablet, a capsule, or a liquid suspension. B.

4. A method according to any one of claims 1 to 3 wherein the amount of cisapride or a pharmaceutically acceptable salt thereof administered is from 1 mg to 200 mg per day. 25

5. A method according to claim 4 wherein the amount administered is from mg to 100 mg per day.

6. A method according to claim 5 wherein the amount administered is from mg to 75 mg per day.

7. A method according to any one of claims 1 to 6 wherein the amount is administered in divided doses from one to four times per day. C:Xwinword AnnaNB NODELETESpecifcationsw677-97.doc -23-

8. A method according to claim 7 wherein said amount is administered in divided doses from one to two times per day.

9. A method according to any one of claims 1 to 8 wherein the amount of cisapride or a pharmaceutically acceptable salt thereof is at least 90% by weight of the total weight of cisapride.

A method according to claim 9 wherein the amount of cisapride or a pharmaceutically acceptable salt thereof is at least 99% by weight of the total weight of cisapride.

11. A method according to claim 9 wherein the amount of cisapride or a pharmaceutically acceptable salt thereof is greater than 99% by weight of the total weight of cisapride.

12. A method according to any one of claims 1 to 11 wherein the amount of said cisapride or a pharmaceutically acceptable salt thereof, substantially free of its stereoisomer is administered together with a pharmaceutically acceptable carrier.

13. A method according to any one of claims 1 to 12 substantially as hereinbefore described with reference to the examples.

14. The use of an amount of cisapride substantially free of its W** S. 25 stereoisomer for the manufacture of a medicament for the treatment of nausea and vomiting.

The use of an amount of cisapride substantially free of its stereoisomer for the manufacture of a medicament for the treatment of nausea and vomiting wherein the amount of cisapride or pharmaceutically acceptable salt thereof is at least 90% by weight of total weight of cisapride.

16. The use of an amount of cisapride substantially free of its f stereoisomer for the manufacture of a medicament for the treatment of nausea C: winwor\Anna\MNB\NODELEE\Specications467797.doc -24- and vomiting wherein the amount of cisapride or pharmaceutically acceptable salt thereof is at least 99% by weight of total weight of cisapride.

17. The use of an amount of cisapride substantially free of its stereoisomer for the manufacture of a medicament for the treatment of nausea and vomiting wherein the amount of cisapride or pharmaceutically acceptable salt thereof is greater than 99% by weight of total weight of cisapride. DATED: 19 January 2000 PHILLIPS ORMONDE FITZPATRICK 4 Attorneys for: O 15 SEPRACOR, INC. 00 9 @0• C:XwinwordAnnaWNB\NODELETE\Specifications'46770-97.do