AU4314099A - Novel method of large scale plasmid purification - Google Patents
Novel method of large scale plasmid purificationInfo
- Publication number
- AU4314099A AU4314099A AU43140/99A AU4314099A AU4314099A AU 4314099 A AU4314099 A AU 4314099A AU 43140/99 A AU43140/99 A AU 43140/99A AU 4314099 A AU4314099 A AU 4314099A AU 4314099 A AU4314099 A AU 4314099A
- Authority
- AU
- Australia
- Prior art keywords
- large scale
- novel method
- plasmid purification
- scale plasmid
- purification
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J41/00—Anion exchange; Use of material as anion exchangers; Treatment of material for improving the anion exchange properties
- B01J41/20—Anion exchangers for chromatographic processes
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
- B01J20/30—Processes for preparing, regenerating, or reactivating
- B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
- B01J20/3231—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the coating or impregnating layer
- B01J20/3242—Layers with a functional group, e.g. an affinity material, a ligand, a reactant or a complexing group
- B01J20/3285—Coating or impregnation layers comprising different type of functional groups or interactions, e.g. different ligands in various parts of the sorbent, mixed mode, dual zone, bimodal, multimodal, ionic or hydrophobic, cationic or anionic, hydrophilic or hydrophobic
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
- C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction, e.g. ion-exchange, ion-pair, ion-suppression or ion-exclusion
- B01D15/361—Ion-exchange
- B01D15/363—Anion-exchange
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 and B01D15/30 - B01D15/36, e.g. affinity, ligand exchange or chiral chromatography
- B01D15/3847—Multimodal interactions
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
Landscapes
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Analytical Chemistry (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biomedical Technology (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Crystallography & Structural Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Peptides Or Proteins (AREA)
Applications Claiming Priority (5)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US8756898P | 1998-06-01 | 1998-06-01 | |
US60087568 | 1998-06-01 | ||
US23846699A | 1999-01-28 | 1999-01-28 | |
US09238466 | 1999-01-28 | ||
PCT/US1999/011588 WO1999063076A1 (en) | 1998-06-01 | 1999-05-26 | Novel method of large scale plasmid purification |
Publications (1)
Publication Number | Publication Date |
---|---|
AU4314099A true AU4314099A (en) | 1999-12-20 |
Family
ID=26777125
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
AU43140/99A Abandoned AU4314099A (en) | 1998-06-01 | 1999-05-26 | Novel method of large scale plasmid purification |
Country Status (2)
Country | Link |
---|---|
AU (1) | AU4314099A (en) |
WO (1) | WO1999063076A1 (en) |
Families Citing this family (22)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7026468B2 (en) | 1996-07-19 | 2006-04-11 | Valentis, Inc. | Process and equipment for plasmid purification |
US7807822B2 (en) | 1996-08-01 | 2010-10-05 | Robert Bridenbaugh | Methods for purifying nucleic acids |
HUP0202295A3 (en) | 1999-07-23 | 2005-01-28 | Genentech Inc | Method for rnase- and organic solvent-free plasmid dna purification using tangential flow filtration |
DE50008101D1 (en) * | 2000-02-16 | 2004-11-11 | Macherey Nagel Gmbh & Co Hg | Process for the purification of nucleic acids and anion exchangers for carrying out this process |
DE10010342A1 (en) | 2000-03-06 | 2001-09-20 | Merck Patent Gmbh | Method for reducing the endotoxin content of nucleic acid (I) is derived from natural, genetic engineering or biotechnological sources is used to produce high-purity plasmid DNA from natural sources |
WO2001094573A2 (en) * | 2000-06-02 | 2001-12-13 | Pall Corporation | Processing of plasmid-containing fluids |
EP1243649A1 (en) * | 2001-03-23 | 2002-09-25 | GeneScan Europe AG | Method for the isolation of DNA |
CA2498518A1 (en) | 2002-09-13 | 2004-03-25 | Valentis, Inc. | Apparatus and method for preparative scale purification of nucleic acids |
US8501402B2 (en) | 2003-03-24 | 2013-08-06 | Boehringer Ingelheim Rcv Gmbh & Co Kg | Methods and devices for producing biomolecules |
DE602004020473D1 (en) * | 2003-06-24 | 2009-05-20 | Organon Nv | SEPARATION OF LIPOPOLYSACCHARIDES FROM LIPOPOLYSACCHARIDE COMPLEXES USING NON-FLAMMABLE SOLVENTS |
GB0323814D0 (en) * | 2003-10-10 | 2003-11-12 | Cobra Biolog Ltd | Purification of biological macromolecules |
SE0400490D0 (en) * | 2004-02-26 | 2004-02-26 | Amersham Biosciences Ab | Plasmid purification |
US7531308B2 (en) * | 2004-04-23 | 2009-05-12 | Sigma-Aldrich Co. | Process for the reduction of endotoxins in a plasmid preparation using a carbohydrate non-ionic detergent with silica chromatography |
EP2220108A2 (en) * | 2007-11-15 | 2010-08-25 | Novo Nordisk A/S | Protein purification and endotoxin removal |
ES2724981T3 (en) | 2014-09-24 | 2019-09-18 | Basf Se | Procedure for the preparation of diesters of terephthalic acid with circulation of the reaction mixture |
MX2017003926A (en) | 2014-09-24 | 2017-06-30 | Basf Se | Method for producing diesters of terephthalic acid with enrichment of recirculated alcohol. |
ES2724982T3 (en) | 2014-09-24 | 2019-09-18 | Basf Se | Procedure for the production of diesters of terephthalic acid with dehydration of recirculation alcohol |
ES2694276T3 (en) | 2014-09-24 | 2018-12-19 | Basf Se | Procedure for the production of terephthalic acid diesters |
DE102016106271B4 (en) | 2016-04-06 | 2018-03-29 | Axagarius Gmbh & Co. Kg | Purification of nucleic acid from a nucleic acid and endotoxin containing sample |
CN111304193A (en) * | 2020-02-28 | 2020-06-19 | 恒瑞源正(上海)生物科技有限公司 | Method for large-scale rapid purification of plasmid DNA |
EP3906982B1 (en) | 2020-05-08 | 2022-03-16 | AXAGARIUS GmbH & Co. KG | Plasma cleaning method with simultaneous depletion of endotoxins |
CN112322612A (en) * | 2020-10-29 | 2021-02-05 | 江苏凯基生物技术股份有限公司 | Plasmid extraction kit and extraction method |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE3644346A1 (en) * | 1986-12-19 | 1987-05-21 | Saeulentechnik Dr Ing Herbert | Matrix-bound crown ether ligands as separating material in the affinity chromatography of nucleic acids |
AR003122A1 (en) * | 1995-05-19 | 1998-07-08 | Merck & Co Inc | A PROCESS FOR ISOLATION AND PURIFICATION OF PLASMIDS IN LARGE SCALE FERMENTATORS AND ISOLATED AND PURIFIED DNA OBTAINED THROUGH SUCH A PROCESS. |
-
1999
- 1999-05-26 WO PCT/US1999/011588 patent/WO1999063076A1/en active Application Filing
- 1999-05-26 AU AU43140/99A patent/AU4314099A/en not_active Abandoned
Also Published As
Publication number | Publication date |
---|---|
WO1999063076A1 (en) | 1999-12-09 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
MK6 | Application lapsed section 142(2)(f)/reg. 8.3(3) - pct applic. not entering national phase |