AU4015901A - Chemokine receptor antagonists and methods of use therefor - Google Patents

Description

AUSTRALIA

PATENTS ACT 1990 DIVISIONAL APPLICATION NAME OF APPLICANT: Millennium Pharmaceuticals, Inc.

ADDRESS FOR SERVICE: DAVIES COLLISON CAVE Patent Attorneys 1 Little Collins Street Melbourne, 3000.

INVENTION TITLE: "Chemokine receptor antagonists and methods of use therefor" The following statement is a full description of this invention, including the best method of performing it known to us: o IP Australia Documents received on.

0 1 MAY 2001 Batch No: Q:\OPER\MKR\GENERA1_\36598.q7-djvdoc 11/01 CHEMOKINE RECEPTOR ANTAGONISTS AND METHODS OF USE THEREFOR BACKGROUND OF THE INVENTION Chemoattractant cytokines or chemokines are a family of proinflammatory mediators that promote recruitment and activation of multiple lineages of leukocytes and lymphocytes. They can be released by many kinds of tissue cells after activation. Continuous release of chemokines at sites of inflammation mediates the ongoing migration of S: 10 effector cells in chronic inflammation. The chemokines characterized to date are related in primary structure.

They share four conserved cysteines, which form disulfide bonds. Based upon this conserved cysteine motif, the family is divided into two main branches, designated as the C-X-C chemokines (a-chemokines), and the C-C chemokines S• (-chemokines), in which the first two conserved cysteines are separated by an intervening residue, or adjacent respectively (Baggiolini, M. and Dahinden, C. A., Immunology Today, 15:127-133 (1994)) The C-X-C chemokines include a number of potent chemoattractants and activators of neutrophils, such as interleukin 8 PF4 and neutrophil-activating peptide-2 (NAP-2). The C-C chemokines include RANTES (Regulated on Activation, Normal T Expressed and Secreted), the macrophage inflammatory proteins la and 13 (MIP-la and MIP-l and human monocyte chemotatic proteins 1-3 (MCP-1, MCP-2, MCP-3), which have been characterized as chemoattractants and activators of monocytes or lymphocytes but do not appear to be chemoattractants for neutrophils.

Chemokines, such as RANTES and MIP-la, have been implicated in a wide range of human acute and chronic inflammatory diseases including respiratory diseases, such as asthma and allergic disorders.

The chemokine receptors are members of a superfamily of G protein-coupled receptors (GPCR) which share structural features that reflect a common mechanism of action of signal transduction (Gerard, C. and Gerard, N.P., Annu Rev. Immunol., 12:775-808 (1994); Gerard, C. and Gerard, N. Curr. Opin. Immunol., 6:140-145 (1994)).

15 Conserved features include seven hydrophobic domains spanning the plasma membrane, which are connected by hydrophilic extracellular and intracellular loops. The majority of the primary sequence homology occurs in the hydrophobic transmembrane regions with the hydrophilic 20 regions being more diverse. The first receptor for the C-C chemokines that was cloned and expressed binds the chemokines MIP-la and RANTES. Accordingly, this MIP-la/RANTES receptor was designated C-C chemokine receptor 1 (also referred to as CCR-1; Neote, et al., .eeo* S• 25 Cell, 72:415-425 (1993); Horuk, R. et al., WO 94/11504, May 26, 1994; Gao, et al., J. Exp. Med., 177:1421-1427 (1993)). Three new receptors have been characterized which bind and/or signal in response to RANTES: CCR3 mediates binding and signaling of chemokines including eotaxin, RANTES, and MCP-3 (Ponath et al., J. Exp. Med., 183:2437 (1996)), CCR4 binds chemokines including RANTES, MIP-la, and MCP-1 (Power, et al., J. Biol. Chem., 270:19495 (1995)), and CCR5 binds chemokines including MIP-la, RANTES, and MIP-1P (Samson, et al., Biochem. 35: 3362-3367 (1996)). RANTES is a chemotactic chemokine for a variety of cell types, including monocytes, eosinophils, and a subset of T-cells. The responses of these different cells may not all be mediated by the same receptor, and it is possible that the receptors CCR1, CCR4 and CCR5 will show some selectivity in receptor distribution and function between leukocyte types, as has already been shown for CCR3 (Ponath et In particular, the ability of RANTES to induce the directed migration of monocytes and a memory population of circulating T-cells (Schall, T. et al., Nature, 347:669-71 (1990)) suggests this chemokine and its receptor(s) may play a critical role in chronic inflammatory diseases, since these diseases are 15 characterized by destructive infiltrates of T cells and -monocytes.

Many existing drugs have been developed as antagonists of the receptors for biogenic amines, for example, as antagonists of the dopamine and histamine receptors. No 20 successful antagonists have yet been developed to the receptors for the larger proteins such as chemokines and C5a. Small molecule antagonists of the interaction between C-C chemokine receptors and their ligands, including RANTES and MIP-la, would provide compounds useful for inhibiting 25 harmful inflammatory processes "triggered" by receptor ligand interaction, as well as valuable tools for the investigation of receptor-ligand interactions.

SUMMARY OF THE INVENTION It has now been found that a number of small organic molecules are antagonists of chemokine receptor function and can inhibit leukocyte activation and/or recruitment.

An antagonist of chemokine receptor function is a molecule which can inhibit the binding of one or more chemokines, -4including C-C chemokines such as RANTES and MIP-la, to one or more chemokine receptors on leukocytes and/or other cell types. As a consequence, processes and cellular responses mediated by chemokine receptors can be inhibited with these small organic molecules. Based on this discovery, a method of treating a subject with a disease associated with aberrant leukocyte recruitment and/or activation is disclosed. The method comprises administering to the subject a therapeutically effective amount of a compound or small organic molecule which is an antagonist of chemokine receptor function. Compounds or small organic molecules which have been identified as antagonists of chemokine receptor function are discussed in detail hereinbelow, and can be used for the manufacture of a medicament for treating or for preventing a disease associated with aberrant leukocyte recruitment and/or activation. The invention also relates to the disclosed compounds and small organic molecules and their use in treating or preventing a disease associated with aberrant leukocytes recruitment 20 and/or activation. The invention also includes pharmaceutical compositions comprising one or more of the compounds or small organic molecules which have been identified herein as antagonists of chemokine function and a suitable pharmaceutical carrier. The invention further 25 relates to novel compounds which can be used to treat an individual with a disease associated with aberrant leukocyte recruitment and/or activation.

BRIEF DESCRIPTION OF THE FIGURES Figures 1A and 1B are histograms illustrating the inhibition by varying concentrations of LS370 and LS374 (also referred to herein as "L-370" and "L-374", respectively) in the chemotaxis of fresh peripheral blood mononuclear cells (PBMC) in response to RANTES or MIP-la.

DETAILED DESCRIPTION OF THE INVENTION The present invention relates to small molecule compounds which are antagonists of chemokine receptor function. Accordingly, processes or cellular responses mediated by the binding of a chemokine to a receptor can be inhibited (reduced or prevented, in whole or in part), including leukocyte migration, integrin activation, transient increases in the concentration of intracellular free calcium [Ca"] i and/or granule release of proinflammatory mediators.

The invention further relates to a method of treatment, including prophylactic and therapeutic treatments, of a disease associated with aberrant leukocyte recruitment and/or activation, including chronic inflammatory disorders characterized by the presence of RANTES and/or MIP-la responsive T cells, monocytes and/or eosinophils, including but not limited to diseases such as arthritis, psoriasis, multiple sclerosis, inflammatory bowel diseases such as ulcerative colitis and Crohn's 20 disease, as well as allergies and asthma. Other diseases associated with aberrant leukocyte recruitment and/or activation which can be treated (including prophylactic treatments) with the methods disclosed herein are inflammatory diseases associated with Human 25 Immunodeficiency Virus (HIV) infection, AIDS associated encephalitis, AIDS related maculopapular skin eruption, AIDS related interstitial pneumonia, AIDS related enteropathy, AIDS related periportal hepatic inflammation and AIDS related glomerulo nephritis. The method comprises administering to a subject a therapeutically effective amount of a compound one or more compounds) which inhibits chemokine receptor function, inhibits the binding of a chemokine to leukocytes and/or other cell types, and/or which inhibits leukocyte migration to, and/or activation at, sites of inflammation. According -6to the method, chemokine-mediated chemotaxis and/or activation of pro-inflammatory cells bearing receptors for chemokines can be inhibited. As used herein, "proinflammatory cells" includes but is not limited to leukocytes, since chemokine receptors may be expressed on other cell types, such as neurons and epithelial cells.

In one embodiment of the present invention, the antagonist of chemokine receptor function is represented by Structural Formula 2

X--N

(I)

Z is a substituted or unsubstituted aromatic group.

Y is a covalent bond, or -CO-.

n is an integer from one to about five. n is preferably one, two, or three.

X is a covalent bond or -CO-.

Ra and R taken together with the nitrogen atom to which they are bonded, form a substituted or unsubstituted non-aromatic heterocyclic ring. For example, Ra and Rb, 20 together with the nitrogen atom to which they are bonded, form a four, five, six, seven or eight-membered nitrogencontaining non-aromatic ring. Alternatively Ra is an aliphatic or a substituted aliphatic group and Rb is an aliphatic group substituted with an aromatic group or substituted aromatic group.

In a preferred embodiment, Ra and Rb, together with the nitrogen atom to which they are bonded, form a six-membered nitrogen-containing non-aromatic ring. For example, the -7six-membered, nitrogen-containing non-aromatic ring can be chosen such that the antagonist of chemokine receptor function is represented by Structural Formula (II): X- N M

(II)

Z, Y, X and n are as described in Structural Formula M is >CRiR 2 or M is preferably 10 >NR 2 or >CRiR 2 RI is -OH, an aliphatic group, -O-(aliphatic group), -SH or -S-(aliphatic group). Preferably, R, is -H or -OH.

R

2 is an aliphatic group, a substituted aliphatic 15 group, an aromatic group, a substituted aromatic group, a benzylic group, a substituted benzylic group, a nonaromatic heterocyclic group or a substituted non-aromatic e* heterocyclic group.

When M is >NR 2 or >CRiR 2 the antagonist of chemokine S 20 receptor function is preferably a compound represented by Structural Formulas (III) through (VIII): -8- (III) (IV) (CH)n (CH 2 )n 0 N 0 N NR2 ^R1

R

2

(VI)

C H 2 n C H n SN0

NQ

NR

5 (VII) (VIII) o: *9 In Structural Formulas (III) and n is preferably one, two or three, more preferably one. When n is one and post R, is -H or -OH, R 2 is preferably a C 1 to about a C 4 alkyl *group substituted with an aromatic or substituted aromatic group.

In Structural Formulas and n is preferably one, two or three, more preferably two or three. When n is two or three and RR is -H or -OH, R 2 is preferably an aliphatic or substituted aliphatic group, preferably an alkyl group substituted with a hydroxyl, alkoxy, thiol, or alkylthio group.

In Structural Formulas (VII) and (VIII), n is preferably one, two or three, more preferably three. When n is three and Ri is -H or -OH, R 2 is preferably an aromatic group, a substituted aromatic group or an aromatic group, a substituted aromatic group or an aliphatic group substituted with an aromatic or substituted aromatic group.

In another preferred embodiment, and in Structural Formula (II) are each a covalent bond and the antagonist of chemokine receptor function is a compound represented by Structural Formula (IX): (CHYrr--N M

(IX)

Z, n and M are as described above for Structural Formula 10 Preferably, Z is a tricyclic ring system comprising two carbocyclic aromatic groups fused to a seven or eight membered cycloalkyl group or to a non-aromatic heterocyclic ring. In one example, Z is represented by Structural Formula (IXa): (IXa) The phenyl rings in Structural Formula (IXa), labeled with an and are referred to herein as "Ring A" and "Ring respectively. The central ring, labeled with a is referred to as "Ring C" and can be, for example a seven or eight membered non-aromatic carbocyclic ring a cycloheptane or cyclooctane ring) or a nonaromatic heterocyclic ring. When Ring C is a non-aromatic heterocyclic ring, it can contain one or two heteroatoms such as nitrogen, sulfur or oxygen. When Z is represented by Structural Formula (IXa), the tricyclic ring system is connected to the alkylene group in Structural Formula (IX) by a single covalent bond between the alkylene group and a ring atom in Ring C which is not also in Ring A or Ring B.

Ring A and/or Ring B can be unsubstituted.

Alternatively, Ring A and/or Ring B can have one or more substituents. Suitable substituents are as described hereinbelow for substituted aromatic groups.

In addition, Ring C optionally contains one or more additional substituents, for example, R 3 and R 4 When Ring C is a non-aromatic carbocyclic ring, substituents such as

R

3 and R 4 are as described hereinbelow for substituted 15 aliphatic rings. When Ring C contains one or more heteroatoms, substituents such as R 3 and R 4 are as described below for non-aromatic heterocyclic rings.

Preferably, R 3 is -H and R 4 is -H or an electron withdrawing group. Suitable electron withdrawing groups 20 include -CN, alkylsulfonyl, carboxamido, carboxylic alkyl esters, -NO. and halogens -Br and -Cl).

More preferably, Z in Structural Formula (IX) is represented by Structural Formulas and (XI): x

(XI)

-11- X, is a chemical bond, -CH 2 or -CH 2

S-.

Preferably, X, is in Structural Formula and -CH 2

S-

in Structural Formula (XI).

W is -H or an electron withdrawing group, as described above for Structural Formula (IXa). A preferred electron withdrawing group is -CN. Ring A and Ring B are as described above in Structural Formula (IXa).

When Xi in Structural Formula is or when X, in Structural Formula (XI) is -CHS-, M is preferably >NR 2 or >CRiR 2 When M is >NR 2 or >CRiR 2 W is preferably -CN and n is preferably two, three or four, more preferably three.

R, is preferably -H or -OH.

In another preferred embodiment, Ra is an aliphatic or a substituted aliphatic group and Rb is an aliphatic group 15 substituted with an aromatic group or substituted aromatic group. As a consequence, the antagonist of chemokine receptor function is a compound represented by Structural Formula (XII): /Ra Z N Rb o 20

(XII)

Preferably, n is an integer from about two to about four; Ra is a C, to about a C 4 substituted or unsubstituted alkyl group; and Rb is -(CH 2 wherein m is an integer from about two to about four, and R 10 is an aromatic group.

In yet another preferred embodiment, the antagonist of chemokine function is a compound represented by Structural Formula wherein Z is represented by Structural Formulas or (XI) and and are each a covalent bond. In this instance the antagonist of chemokine receptor function is a compound represented by Structural Formulas (XIII) or (XIV): -12- W (CH Ra vv (CH 7 Ra N N Rb Rb (XIII) (XIV) In Structural Formulas (XIII) and (XIV), X 1 is as defined above for Structural Formulas and n is an integer 5 from two to five; W is -CN, alkylsulfonyl, carboxamido or carboxyalkyl; In Structural Formulas (XIII) and (XIV), Ring A is substituted with R, and R 9 wherein R, and R 9 are independently a halogen, alkoxy or alkyl, or, taken together with ring A, form a naphthyl group; and R a and Rb oe are independently alkyl, aralkyl or, taken together with the nitrogen atom bonded to Ra and Rb, form a nonaromatic heterocyclic ring represented by a structure selected from: -13- 0 OH HO R 6

R

N N N N

R

and(N N N N R, is alkanoyl, aroyl, aralkoyl, alkyl, aralkyl or cycloalkanoyl.

R

6 is an aryl group.

R7 is -H or a heterocylic ring.

In another embodiment of the present invention, the antagonist of chemokine receptor function is represented by Structural Formula (XVI): -14- A n

R

13

R

11

(XVI)

A is >NR, 1 -CH2-, -CH(R 14 or 5 -C (R14R1)-.

R is halogen, -CN, an aliphatic group, a substituted aliphatic group, an aromatic group, a substituted aromatic group, -OH, -O-(aliphatic group), -0- (substituted aliphatic group), -S-(aliphatic group), -S- (substituted aliphatic group), -NO 2

-NH

2 -NH(aliphatic group), -NH(substituted aliphatic group), -N(aliphatic group) 2 -N(substituted aliphatic group) 2

R

12 is an aromatic group or an aliphatic group.

Each R 1 3 is independently chosen and is an aliphatic group or substituted aliphatic group. Thus, if n is greater than one, the R 13 attached to one double bond can be the same as or different from the R 13 substituents attached to the other double bonds. Structural Formula (XVI) indicates that each Ri3 can be bonded to either carbon atom in the double bond and that the stereochemistry of each double bond is independently selected and can be cis or trans.

n is an integer from one to about four.

B is -N(R 6 or a covalent bond.

R

14

R

15 and R 16 are independently an aliphatic group or a substituted aliphatic group and can be the same or different.

Q is an aliphatic group, a substituted aliphatic group, a benzylic group, a substituted benzylic group, an aromatic group, a substituted aromatic group, a nonaromatic heterocyclic group or a substituted non-aromatic heterocyclic group.

In a preferred embodiment, n is 1 and B and Q are as defined above. In this instance, A is preferably -Sor >C(CH 3 2 B is -N(R, 6 or a covalent bond and R, 3 is preferably -H or, when B is an aliphatic or substituted aliphatic group bonded to the same olefinic carbon atom as sulfur. As a consequence, the antagonist of 15 chemokine receptor function is a compound represented by one of Structural Formulas (XVII) through (XXV): R12 R12 (XVII) (XVIII)/ S 0

R

S" (XVII) (XVIII)

RR

12 R2 (XIX) (XX) -16-

N+Q

N.S

(XXI) (XX II) 00 6000 000.

(XXI II) (XXIV)

(XXV)

In Structures (XVII) through (XXV) R 1 3 and R 16 are preferably an aliphatic group.

Alternatively, in Structural Formula XVI, B, Q and the terminal olef in carbon, taken together, form a non-aromatic -17heterocyclic ring. The antagonist of chemokine receptor function is then represented by Structural Formula (XXVI): R12 o ooo.

-oo: :o o o..o .11.

0.0.

.0.00 0.00.

(XXVI)

R

1

R

12

R

13 and n are as described above for Structural Formula (XVI). Optionally, the non-aromatic heterocyclic ring in Structural Formula (XXVI), designated with a "D" and referred to herein as "Ring can be fused to an aromatic ring or substituted aromatic ring. The non- 10 aromatic heterocyclic ring can be substituted or unsubstituted. In one example, Ring D is represented by the following structural formula: In another embodiment of the present invention, the antagonist of chemokine receptor function is represented by Structural Formula (XXVII): -18- R21 R23

(XXVII)

R

n 2 is -OH, an aliphatic group, a substituted aliphatic group, -0-(aliphatic group), -0-(substituted aliphatic group), -O-CO-(aliphatic group) or -O-CO-(substituted aliphatic group) Preferably, R 21 is -OH, CH 3 CO-O- or an alkyl group substituted with CH 3

NH-

an alkyl group substituted at the benzylic carbon 10 atom with methylamino methylene). Examples of R 21 include -OH, CH 3 CO-O- or -CH(-CH(CH 3 2

(-CH

2

NHCH

3

R

22 and R 23 are independently an aliphatic group, a substituted aliphatic group, an aromatic group, a Ssubstituted aromatic group, -S-(aliphatic group), -S- (substituted aliphatic group), (aliphatic group), -0- (substituted aliphatic group), (CH 2 )n-R 26 and, taken together, can be a -(CH 2 2 to -(CH 2 5 alkylene group or a

-(CH

2 2 to -(CH 2 5 alkylene group substituted with one or more aliphatic groups, substituted aliphatic groups, 20 aromatic groups or substituted aromatic groups.

Preferably, R 22 is thioalkyl, alkyl or phenyl and R 23 is -H, methyl or, taken together with R 22 a propylene group. The propylene group can be unsubstituted or substituted with one or more methyl or ethyl groups. Examples of R 22 include -SCH 15 methyl or phenyl. Examples of R 23 include methyl or, taken together with R 22 a -CH 2

CH

2

C(CH

3 2 group.

R

26 is a substituted or unsubstituted aromatic group.

-19- In one aspect, the antagonist of chemokine receptor function is a compound represented by Structural Formula (XXVII), wherein:

R

21 is -OH, an alkyl group, an alkoxy group, an acetoxy group or an alkyl group substituted with -NR 24

R

2 5

R

22 and R 23 are independently an alkyl group, an aromatic group, an aralkyl group, and ethylene-R 6 or thioalkyl, and, taken together, form an alkylene group;

R

24 and R 25 are independently an alkyl group, an aralkyl group and an aryl group;

R

26 is a phenyl group substituted by R, and R 2 8; and

R,

2 and are independently -OH, alkoxy, or halogen.

In another embodiment of the present invention, the I 15 antagonist of chemokine function is a compound represented by Structural Formula (XXVIII): :4 4 2 R43

(XXVIII)

R,

0 and R 43 are independently an aliphatic group, a 20 substituted aliphatic group, a benzylic group, a substituted benzylic group, an aromatic group, a substituted aromatic group, a non-aromatic heterocyclic group or a substituted non-aromatic heterocyclic group.

R

41 and R 42 are independently an aliphatic group or a substituted aliphatic group. Preferably, R 41 and R 42 are each a methyl group.

In another embodiment of the present invention, the antagonist of chemokine receptor function is a compound represented by Structural Formula (XXIX): R53 R52 R0 R51 R52 R53

(XXIX)

R,

5 and R, 5 are independently -OH, a halogen, -0- (aliphatic group), -0-(substituted aliphatic group), -0-CO- S 5 (aliphatic group), -0-CO-(substituted aliphatic group),

-NH

2 -NH(aliphatic group), -NH(substituted aliphatic group), -N(aliphatic group) 2 -N(substituted aliphatic group) 2 -S-(aliphatic group) or -S-(substituted aliphatic group. Preferably, R 50 and R, 5 are independently -OH, a 10 halogen, -O-(aliphatic group) or -0-(substituted aliphatic group).

R

52 and Rs3 are independently an aliphatic group, a :substituted aliphatic group, a halogen, -NH 2 -NH(aliphatic group), -NH(substituted aliphatic group), -N(aliphatic group) 2 or -N(substituted aliphatic group) 2 Preferably,

R

52 and R 53 are independently an aliphatic group, a substituted aliphatic group or a halogen.

Also included in the present invention are physiologically acceptable salts of the compounds represented by Structural Formulas through (XXIX).

Salts of compounds containing an amine or other basic group can be obtained, for example, by reacting with a suitable organic or inorganic acid, such as hydrogen chloride, hydrogen bromide, acetic acid, perchloric acid and the -21like. Compounds with a quaternary ammonium group also contain a counteranion such as chloride, bromide, iodide, acetate, perchlorate and the like.

As used herein, aliphatic groups include straight chained, branched or cyclic Ci-C, hydrocarbons which are completely saturated or which contain one or more units of unsaturation.

An "alkyl group" is a saturated aliphatic group, as defined above. The term "alkoxy" refers to an alkyl ether chain with an alkyl group. "Alkanoyl" refers to alkyl substituted carbonyl; "aralkanoyl" refers to phenyl-alkyl-CO- and "aroyl" refers to arylcarbonyl including benzoyl, naphthoyl and the like. The term "halogen" means fluoro, chloro, bromo and iodo. The term 15 "aryl", as opposed to the term "aromatic group", means phenyl. The term "substituted phenyl" means aryl substituted by alkyl, halogen, alkoxy, nitro, amino, acetamido, cyano and trifluoromethyl and naphthyl.

"Aralkyl" means -(CH 2 )x-phenyl, wherein x is an integer from 20 one to four including benzyl. It is noted that the terms "aromatic group", "carbocylic aromatic group" and "heterocyclic aromatic group" are defined below and have different meanings from the term "aryl".

Aromatic groups include carbocyclic aromatic groups such as phenyl, l-naphthyl, 2-naphthyl, 1-anthracyl and 2anthacyl, and heterocyclic aromatic groups such as Nimidazolyl, 2-imidazole, 2-thienyl, 3-thienyl, 2-furanyl, 3-furanyl, 2-pyridyl, 3-pyridyl, 4-pyridyl, 2-pyrimidy, 4pyrimidyl, 2-pyranyl, 3-pyranyl, 3-pyrazolyl, 4-pyrazolyl, 5-pyrazolyl, 2-pyrazinyl, 2-thiazole, 4-thiazole, thiazole, 2-oxazolyl, 4-oxazolyl and Aromatic groups also include fused polycyclic aromatic ring systems in which a carbocyclic aromatic ring or heteroaryl ring is fused to one or more other heteroaryl -22rings. Examples include 2-benzothienyl, 3-benzothienyl, 2-benzofuranyl, 3-benzofuranyl, 2-indolyl, -3-indolyl, 2quinolinyl, 3-quinolinyl, 2-benzothiazole, 2-benzooxazole, 2-benzimidazole, 2-quinolinyl, 3-quinolinyl, 1isoquinolinyl, 3-quinolinyl, 1-isoindolyl, 3-isoindolyl, and acridintyl. Also included within the scope of the term "4aromatic group", as it is used herein, is a group in which one or more carbocyclic aromatic rings and/or heteroaromatic rings are fused to a cycloalkyl or nonaromatic heterocyclic ring. Examples include decalin, phthalimido, benzodiazepines, benzooxazepines, benzooxazines, phenothiazines, and groups represented by the following structural formulas: 0 -23- -24- Non-aromatic heterocyclic rings are non-aromatic carbocyclic rings which include one or more heteroatoms such as nitrogen, oxygen or sulfur in the ring. The ring can be five, six, seven or eight-membered. Examples include 2-tetrahydrofuranyl, 3-tetrahydrofuranyl, 2-tetrahyrothiophenyl, 3-tetrahyrothiophenyl, 2-morpholino, 3-morpholino, 4-morpholino, 2-thiomorpholino, 3-thiomorpholino, 4-thiomorpholino, 1-pyrrolidinyl, 2pyrrolidinyl, 3-pyrrolidinyl, 1-piperazinyl, 2-piperazinyl, 1-piperidinyl, 2-piperidinyl, 3-piperidinyl, 4-piperidinyl and 4-thiazolidinyl.

"Heterocyclic ring", as opposed to "heteroaryl group" and "non-aromatic heterocylic ring", is defined as imidazole, benzimidazole, pyridine, pyrimidine, thiazole, S 15 benzothiazole, thienyl, benzothienyl. It is noted further the terms "heterocyclic aromatic group" and "non-aromatic heterocyclic ring" are defined above and have different meanings from the term "heterocyclic ring".

Suitable substituents on an alkyl, aliphatic, 20 aromatic, non-aromatic heterocyclic ring or benzyl group include, for example, -OH, halogen -Cl, -I and -F) -O(aliphatic, substituted aliphatic, benzyl, substituted benzyl, aromatic or substituted aromatic group), -CN, -NO 2 -COOH, -NH 2 -NH(aliphatic group, substituted aliphatic, 25 benzyl, substituted benzyl, aromatic or substituted aromatic group), -N(aliphatic group, substituted aliphatic, benzyl, substituted benzyl, aromatic or substituted aromatic group) 2 -COO(aliphatic group, substituted aliphatic, benzyl, substituted benzyl, aromatic or substituted aromatic group), -CONH 2 -CONH(aliphatic, substituted aliphatic group, benzyl, substituted benzyl, aromatic or substituted aromatic group)), -SH, -S(aliphatic, substituted aliphatic, benzyl, substituted benzyl, aromatic or substituted aromatic group) and -NH-

C(=NH)-NH

2 A substituted non-aromatic heterocyclic ring, benzylic group or aromatic group can also have an aliphatic or substituted aliphatic group as a substituent. A substituted alkyl or aliphatic group can also have a nonaromatic heterocyclic ring, benzyl, substituted benzyl, aromatic or substituted aromatic group as a substituent. A substituted non-aromatic heterocyclic ring can also have =NH or =N(aliphatic, aromatic or substituted aromatic group) as a substituent. A substituted aliphatic, substituted aromatic, substituted non-aromatic heterocyclic ring or substituted benzyl group can have more than one substituent.

In the structural formulas depicted herein, the single or double bond by which a chemical group or moiety is connected to the remainder of the molecule or compound is 5 indicated by the following symbol: o..

For example, the corresponding symbol in Structural Formula or (XI) indicates that the tricyclic ring system, which respresents Z in Structural Formula is connected to the alkylene group in Structural Formula (IX) by a single covalent bond between the alkylene group and the ring carbon in Ring C which is bonded to W.

A "subject" is preferably a mammal, such as a human, but can also be an animal in need of veterinary treatment, domestic animals dogs, cats, and the like), farm animals cows, sheep, pigs, horses, and the like) and laboratory animals rats, mice, guinea pigs, and the like).

A "therapeutically effective amount" of a compound is an amount which results in the inhibition of one or more processes mediated by the binding of a chemokine to a receptor in a subject with a disease associated with -26aberrant leukocyte recruitment and/or activation. Examples of such processes include leukocyte migration, integrin activation, transient increases in the concentration of intracellular free calcium [Ca 2 and granule release of proinflammatory mediators. Alternatively, a "therapeutically effective amount" of a compound is a quantity sufficient to achieve a desired therapeutic and/or prophylactic effect, such as an amount which results in the prevention of or a decrease in the symptoms associated with a disease associated with aberrant leukocyte recruitment and/or activation.

The amount of compound administered to the individual will depend on the type and severity of the disease and on the characteristics of the individual, such as general health, age, sex, body weight and tolerance to drugs. It will also depend on the degree, severity and type of disease. The skilled artisan will be able to determine appropriate dosages depending on these and other factors.

Typically, a therapeutically effective amount of the compound can range from about 0.1 mg per day to about 100 mg per day for an adult. Preferably, the dosage ranges from about 1 mg per day to about 100 mg per day. An antagonist of chemokine receptor function can also be administered in combination with one or more additional 25 therapeutic agents, e.g. theophylline, 3-adrenergic bronchdilators, corticosteroids, antihistamines, antiallergic agents and the like.

The compound can be administered by any suitable route, including, for example, orally in capsules, suspensions or tablets or by parenteral administration.

Parenteral administration can include, for example, systemic administration, such as by intramuscular, intravenous, subcutaneous, or intraperitoneal injection.

The compound can also be administered orally dietary), topically, by inhalation intrabronchial, -27intranasal, oral inhalation or intranasal drops), or rectally, depending on the disease or condition to be treated. Oral or parenteral administration are preferred modes of administration.

The compound can be administered to the individual in conjunction with an acceptable pharmaceutical carrier as part of a pharmaceutical composition for treatment of HIV infection, inflammatory disease, or the other diseases discussed above. Formulation of a compound to be administered will vary according to the route of administration selected solution, emulsion, capsule). Suitable pharmaceutical carriers may contain inert ingredients which do not interact with the compound.

Standard pharmaceutical formulation techniques can be employed, such as those described in Remington's Pharmaceutical Sciences, Mack Publishing Company, Easton, PA. Suitable pharmaceutical carriers for parenteral administration include, for example, sterile water, physiological saline, bacteriostatic saline (saline 20 containing about 0.9% mg/ml benzyl alcohol), phosphatebuffered saline, Hank's solution, Ringer's-lactate and the like. Methods for encapsulating compositions (such as in a coating of hard gelatin or cyclodextran) are known in the art (Baker, et al., "Controlled Release of Biological 25 Active Agents", John Wiley and Sons, 1986).

The activity of compounds of the present invention can be assessed using suitable assays, such as receptor binding assays and chemotaxis assays. For example, as described in Exemplification Section, small molecule antagonists of RANTES and MIP-la binding have been identified utilizing (butyric acid differentiated) cells which bind RANTES and chemotax in response to RANTES and MIP-la as a model for leukocyte chemotaxis. Specifically, a high through-put receptor binding assay, which monitors 125 1-RANTES and -28- 1 25 I-MIP-la binding to HL-60 cell membranes, was used to identify small molecule antagonists which block binding and RANTES andMIP-la mediated HL-60, T-cell, peripheral blood mononuclear cell, and eosinophil chemotactic response.

Compounds of the present invention can also be identified by virtue of their ability to inhibit the activation steps triggered by binding of a chemokine to its receptor, such as chemotaxis, integrin activation and/or granule mediator release.

The compounds represented by Structural Formula (IX), wherein Z is represented by Structural Formulas (IXa), (X) and (XI) and compounds represented by Structural Formulas (XIII) and (XIV) can be prepared according to methods described in Collect. Czech. Chem. Commun., 50(5):1089-96 15 (1985) (CA 104:33990) and Czech Patent CS 240698 B1 870601 (CA 109:92794). The teachings of these references and references cited therein are incorporated herein by reference. For example, these compounds can be prepared by the following reaction scheme: -29- H N 0= MgBrR H

NCD<OH

R

Compounds represented by Structural Formula and for example, the compounds designated in Table 1 as L-380 and Table 2 as L-372, can be prepared according to methods described in Collect. Czech. Chem. Commun., 54(7):1966-1978 (1989), Czech Patent CS-268400 (1991) and WO 90/13539, the teachings of which are incorporated herein by reference.

Compounds represented by Structural Formula (VII) and (VIII), for example, the compound designated as L-348 in Table 2, can be prepared according to methods described in Synth. Commun. 25(2):177-82 (1995), Chem. Lett., (12):2295- 8 (1994), Ther. Drug. Monit. 10(2):177-83 (1988), J. Med.

Chem. 28(9):1319-24 (1985), U.S. Patent 4,086,234, U.S.

Patent 4,012,514, U.S. Patent 3,936,468, U.S. Patent 15 3,922,266 and U.S. Patent 3,907,812, the teachings of which are incorporated herein by reference.

Compounds represented by Structural Formula (III) and for example the compound designates as L-377 in Table 2, can be prepared according to methods well known in the field of organic chemistry, for example, by reacting the sodium salt of a suitable phenol and a suitable alkylating agent. The phenol is preferably substituted with electron withdrawing groups 3,4,5-trimethoxyphenol). This reaction is shown schematically below: -31- The phenol in the scheme above is preferably substituted with one or more electron withdrawing groups. The alkylating agent prepared, for example, by reacting a oo"o 5 suitable bromo substituted acyl bromide bromoacetyl bromide) with a suitable 1-substituted piperazine, for example, l-benzylpiperazine, as shown below: 0 C Br' H Br HN

(CH

2

N

0 Br',_

(CH

2 )n N -32- Compounds represented by Structural Formula (XII), for example, the compound designated L-347 in Table 1, can be prepared according to methods described in WO 97/11938, WO 97/09983, WO 96/40097, WO 96/39407 and EP 694543, the teachings of which are incorporated herein by reference.

Compounds represented by Structural Formula (XVIII) and (XXI), for example the compound designated L-344 in Table 2, can be prepared, for example, by reacting a 1,3,3trialkylindolinium anion with a suitable alkylating agent according to methods described in European Patent 94 EP 0400348 and U.S. Patent No. US 5,258,274, the teachings of which are incorporated herein by reference. By replacing the 1,3,3-trialkylindolinium anion with an 15 appropriate l-alkyl-benzoxazolinium anion or 1-alkylbenzothiazolinium anion, similar procedures can be used to prepare compound represented by Structural Formulas (XVII), (XIX), (XX) and (XXII) through (XXV) compounds designated as L-459 and L-464 in Table II). These procedures are also suitable for preparing compounds represented by Structural Formula (XXVI), for example, the compound designated L-342 in Table 2, by using an appropriate alkylating agent.

Compounds represented by Structural Formula (XXVII), 25 for example, the compound designated L-381 in Table 1, can be prepared according to methods described in EP 757982, EP 533056, EP 457701, EP 434093 and EP 332064, the teachings of which are incorporated herein by reference. Other compounds represented by Structural Formula (XXVII), for example, the compound designated L-345 in Table 1, can be prepared according to methods described in Sb. Pr. Vyzk. Chem. Vyuziti Uhli, Dehtu Ropy 7:21-39 (1967), Z. Naturforsch. B: Anorg. Chem. Org. Chem 34B(4):624-32 (1979) and J. Med. Chem. 26(6):823-31 (1983), -33the teachings of which are incorporated herein by reference. Yet other compounds represented by Structural Formula (XXVII), for example, the compound designated L-349 in Table 1, can be prepared according to methods described in EP 707007, WO 9501326, EP596692, EP 587050, EP 540165 and CA 2028031, the teachings of which are incorporated herein by reference.

Compounds represented by Structural Formula (XXVIII), for example, the compound designated L-339 in Table 1, can be prepared according to methods described in WO 94/26302, Collect. Czech. Chem. Commun. 53 :1424-60 (1988), EP 226448, ES 540861 and Bull. Chem. Soc. Jap 44(6):1560-2, the teachings of which are incorporated herein by reference.

15 Compounds represented by Structural Formula (XXIX), for example, the compound designated L-319 in Table 1, can be prepared according to methods described in JP 09110771, Polym. Mater. Sci. Eng. 70:378-9 (1993), JP 03148232, JP 02286642, JP 03386641, JP 02248954, EP 342035, EP 307951, Eur. Polym. J. 15(7):631-8 (1979), FR 2322161, Izv. Akad.

Nauk. SSSR. Ser. Khim. (12):2808-10 (1973) and Tetrahedron Lett. (34):3707:10 (1968), the teachings of which are incorporated herein by reference.

The invention is illustrated by the following examples which are not intended to be limiting in any way.

EXEMPLIFICATION

Human eosinophils were prepared by isolation from the blood of donor individuals with high levels of circulating blood eosinophils by combining density gradient centifugation and negative selection with anti-CD16 magnetic beads (Hansel, T.T. J. Immunol. Methods, 122:97- -34- 103 (1989)). Briefly, the granulocyte fraction from the Percoll centrifugation was incubated with CD16 micro beads (miniMACS, separation unit) for 30 minutes. Cells were then passed through a MACS column (Miltenyi Biotec, Inc., Auburn, CA) and eosinophils were collected in the flow through. Eosinophil purity was >99% as determined by analysis of Diff-Quik (Baxter) stained cytocentrifugation preparations by light microscopy.

Cells, obtained from the American Type Culture Collection, were resuspended at 0.5 million cells/ml in equal proportions of RPMI-1640 and M199 (Gibco) with fetal calf serum (FCS). After, addition of n-butyric acid (Sigma Chemical Co.) to a final concentration of 0.4 mM, S..cells were incubated for 4 days at 37 0 C, 5%CO, before use 15 in either whole cell chemotaxis assays or preparation for use as membranes for receptor binding assays.

Membrane Preparations for Chemokine Binding and Binding Assays Membranes were prepared from n-butyric acid-treated 20 cells. Cells were harvested by centrifugation, washed twice with PBS (phosphate-buffered saline), and the cell pellets were frozen at -70 to -85 0 C. The frozen pellet was thawed in ice-cold lysis buffer consisting of 5 mM HEPES (N-2-hydroxyethylpiperazine-N'-2-ethane-sulfonic acid) pH 7.5, 2 mM EDTA (ethylenediaminetetraacetic acid), 5 Ag/ml each aprotinin, leupeptin, and chymostatin (protease inhibitors), and 100 Ag/ml PMSF (phenyl methane sulfonyl fluoride also a protease inhibitor), at a concentration of 1 to 5 x 10 7 cells/ml. This procedure results in cell lysis. The suspension was mixed well to resuspend all of the frozen cell pellet. Nuclei and cell debris were removed by centrifugation of 400 x g for 10 minutes at 4 0

C.

The supernatant was transferred to a fresh tube and the membrane fragments were collected by centrifugation at 25,000 x g for 30 minutes at 4 0 C. The supernatant was aspirated and the pellet was resuspended in freezing buffer consisting of 10 mM HEPES pH 7.5, 300 mM sucrose, l~g/ml each aprotinin, leupeptin, and chymostatin, and 10 Ag/ml PMSF (approximately 0.1 ml per each 108 cells). All clumps were resolved using a minihomogenizer, and the total protein concentration was determined using a protein assay kit (Bio-Rad, Hercules, CA, cat #500-0002). The membrane solution was then aliquoted and frozen at -70 to -85 0

C

until needed.

*h S Binding Assays utilized the membranes described above.

Membrane protein (2 to 20 Mg total membrane protein) was 15 incubated with 0.1 to 0.2 nM 12 5I-labeled RANTES or MIP-la with or without unlabeled competitor (RANTES or MIP-la) or various concentrations of compounds. The binding reactions were performed in 60 to 100 Al of a binding buffer '.consisting of 10 mM HEPES pH 7.2, 1 mM CaC1 2 5 mM MgC1 2 and 0.5% BSA (bovine serum albumin), for 60 min at room temperature. The binding reactions were terminated by harvesting the membranes by rapid filtration through glass fiber filters (GF/B or GF/C, Packard) which were presoaked in 0.3% polyethyleneimine. The filters were rinsed with approximately 600 Ml of binding buffer containing 0.5 M NaC1, dried, and the amount of bound radioactivity was determined by scintillation counting in a Topcount betaplate counter.

Chemokines and Chemotaxis.

RANTES and MIP-la were purchased from Peprotech, Inc.

Leukocyte chemotaxis was assessed on eosinophils, peripheral blood mononuclear cells, or HL60 cells -36differentiated with butyric acid, using a modification of a transendothelial assay (Carr, et al. Proc. Nati Acad Sci, USA, 91, 3652 (1994)). The endothelial cells used in this assay were the endothelial cell line, ECV 304, obtained from the European collection of Animal Cell Cultures (Porton Downs, Salisbury, Endothelial cells were cultured on 6.5 mm diameter Transwell culture inserts (Costar Corp., Cambridge,MA) with 3.0 gm pore size.

Culture media for the ECV 304 cells consisted of M199+10% FCS, L-glutamine, and antibiotics. The assay media consisted of equal parts RPMI 1640 and M199 with 0.5% BSA.

Two hours before the assay, 2x10 5 ECV 304 cells were plated onto each insert of the 24 well Transwell chemotaxis plate and incubated at 37 0 C. Chemotactic factors such as RANTES or MIP-la (Peprotech) (diluted in assay medium) were added to the 24-well tissue culture plates in a final volume of 600 iL. Endothelial-coated Transwells were inserted into .each well and 106 cells of the leukocyte type being studied 9 were added to the top chamber in a final volume of 100gL of assay medium. The plate was then incubated at 37 0 C in C0 2 /95% air for l-2h. The cells that had migrated to the bottom chamber were counted using flow cytometry. 500AL of the cell suspension from the lower chamber was placed in a tube and relative counts were obtained for a set period of 25 time of 30 seconds. This counting method was found to be highly reproducible and enabled gating on the leukocytes and the exclusion of debris or other cells. Counts obtained by this method matched closely those obtained by counting with a microscope. Assays evaluating chemotaxis inhibitors were performed in the same way as control experiments above, except that inhibitor solutions, in assay media containing up to 1% of DMSO cosolvent, were added to both the top and bottom chambers prior to addition of the cells.

Inhibitor potency was determined by comparison of cell -37numbers migrated to the bottom chamber, with or without inhibitor. Control wells contained equivalent amounts of DMSO, but no inhibitor.

Ligand Binding Assay.

125I-RANTES and 125 I-MIP-la were purchased from DuPont-NEN (Boston,MA) with a specific activity of 2,200 Ci/mM.

Chemokine binding to the target cells, human eosinophils, was carried out using a modification of a method previously reported.( Van Riper, J. Exp. Med. 177, 851-856 (1993)). Cells were washed once in PBS and resusupended in binding buffer (50mM HEPES, 1 mM CaC1 2 5 mM MgC1 2 and 0.5% BSA) at a concentration of 1x10 7 mL. Aliquots of L (5x10 5 cells) were dispensed into microfuge tubes, followed by the addition of cold and radiolabelled 15 chemokines. The final reaction volume was 200 AL.

Nonspecific binding was determined by incubating cells with radiolabeled chemokines in the presence of increasing amounts of (250-500 nM) of cold chemokine. After incubation, at room temperature, the cells were washed 3x with 1 mL of binding buffer plus 0.5 M NaCl. Cell pellets were then counted. All experiments were carried out using duplicates and repeated at least three times. Curve fit was calculated by Kaleidagraph software (Synergy Software, Reading, PA). Inhibition of binding was assessed by the 25 addition of test inhibitor compound at concentrations of !00 AM final concentration, and incubation for 30 min prior to addition of the chemokine as above.

Inhibition of Peripheral Blood Mononuclear Cell (PBMC) Chemotaxis By Compounds L-370 and L-374 Cells were incubated with the concentrations of compound indicated in Figures 1A and 1B for 20 minutes at room temperature and were placed in the upper wells of the -38chemotaxis chambers. Migration in response to MCP-1, RANTES, or MIP-la was assessed as described above.

Figure 1A is an illustration of the total number of cells migrating in response to the chemokines with and without preincubation with different concentrations of L- 370 or L-374. MCP-1 was used as a negative control to show the specificity of action of the compounds.

Figure 1B is an illustration of the results of the same experiments as in Figure 1A, expressed as percentage inhibition, where the inhibition was calculated as cells migrated in the absence of compound/cells migrated in the presence of compound. 100% inhibition of migration occurred with 10.0 AM and 1.0 AM of L-370 and L-374, respectively.

15 The activities of other test compounds are reported in Tables 1-4 below as RBA, ICso or the inhibitor concentration required for 50% inhibition in receptor binding assays using 125I-RANTES or 125 MIP-la as ligand and "i HL60 cell membranes from cells differentiated by butyric 20 acid (which chemotax in response to RANTES in an almost identical way described for eosinophils).

Leukocyte chemotaxis inhibition is expressed as percent inhibition of RANTES-induced chemotaxis using the same S"cells (butyric acid diffentiated) at the indicated concentration (pM) of compound.

-39- Table 1

H

H

3

C.N

Icso W Receptor Bind.

1Rantes HIP-a Chemotaxis Inh %TnHib La pM (EmGO)

C

HO

HOa CH3

HCO

HOCH

0I H3C

NN

H3

N

HH

3

C

rN-)OH K-T~ H 3 c. 2H

CH

3 L-381 L-319 L-345 L-349 L-347 L-380 L-339 11 12 72% 9 2.5 PM 100% 0 5 pM 2.4 9 31% 0 10 PM 9 12 56% 8 pM 18 10 not tested 12 7.6 21 12 IiM Q60 pM 14 8 not tested 10 nt. 5%@30pM Table 2 L# RBA IC50 Rantes MIP-1ca L-377 2 0.6

OH~

Inhibition aiemxis %Inhib'n.;pM 66%LalOpM LH I-339 10 23 not tested 0 H OQ.O)H.-3 L-372 5-5 17 L-348 8 10 L-342 6 =-12 1,344 5 15 LA~-59 3 13 L-464 35 =-10 89%e6jiM 54%Q.4p.M 103 74%@2pM 67%@ 2pM 92%1@ not tested H +3C) Nt N

CH

3 -41- Table 3 RBA IC50 W) RANTES MP-la N CH 3

S

S

S

S0 cQ N7R.NH 0

Q

NaNH 0 L-886 L-804 L,374 L-370 L-887 L-378 11.3 >20 0.2 7.3 >40 21 11.2 not tested 0.36 11.7 not tested 33 koyte Chmomxi (L6O Cells) Inhibition PM not tested not tested 81% @IIM 59 %621M I0%gao IIM not tested not tested -42- Table 4 INMMlBON OFEOTAXIN-INDUCE QSNOPHIL HEMOTAXMs *o 0 0000 o* *O S. 0* 00% 00 oNo -<b L-34 L-377 L-370 L.-374 EosinophllGemouais InI~ibion 1 tpM 17% /17 ILM 86% 135 11M 100% [3 pLM 100% 6 pM 26%1/2 p1M 1 10 pLM IC50 45.5 piM -43- Equivalents Those skilled in the art will be able to iecognize, or be able to ascertain, using no more than routine experimentation, many equivalents to the specific embodiments of the invention described herein. Such equivalents are intended to be encompassed by the following claims.

0 *0

Claims (55)

1. A method of treating a subject with a disease associated with aberrant leukocyte recruitment and/or activation comprising administering to the individual a therapeutically effective amount of a compound represented by the following structural formula: N Rb Sand physiologically acceptable salts thereof, wherein: Z is a substituted or unsubstituted aromatic group; Y is a covalent bond, or -CO-; n is an integer from one to about five; X is a covalent bond or and Ra is an aliphatic or a substituted aliphatic group; and Rb is an aliphatic group substituted with an aromatic group or substituted aromatic group; and wherein Ra and Rb, taken together with the nitrogen atom bonded to Ra and Rb, can form a substituted or unsubstituted non-aromatic heterocyclic ring.

2. The method of Claim 1 wherein the compound is represented by the following structural formula: M and physiologically acceptable salts thereof, wherein: M is >NR 2 >CRiR 2 or -CO-; R, is -OH, an aliphatic group, -0-(aliphatic group), -SH or -S-(aliphatic group); R 2 is an aliphatic group, a substituted aliphatic group, an aromatic group, a substituted aromatic group, a benzylic group, a substituted benzylic group, S. 10 a non-aromatic heterocyclic group or a substituted non-aromatic heterocyclic group.

3. The method of Claim 2 wherein the compound is represented by the following structural formula: NR 2 and physiologically acceptable salts therof.

4. The method of Claim 3 wherein is and is -CO-.

5. The method of Claim 4 wherein n is one and R 2 is a C1 to about a C4 alkyl group substituted with an aromatic or substituted aromatic group.

6. The method of Claim 2 wherein the compound is represented by the following structural formula: -46- R1 n 10R2 and physiologically acceptable salts thereof, wherein RI is -H or -OH.

7. The method of Claim 6 wherein is and is -CO-.

8. The method of Claim 7 wherein n is one and R 2 is Cl to about a C4 alkyl group substituted with an aromatic or substituted aromatic group. The method of Claim 3 wherein the compound is 10 represented by the following structural formula: 0 (CH NR 2 and physiologically acceptable salts thereof. The method of Claim 9 wherein n is 2 or 3 and R 2 is an aliphatic or substituted aliphatic group.

11. The method of Claim 9 wherein the compound is represented by the following structural formula: -47- 0 (CH2 -OH and physiologically acceptable salts thereof.

12. The method of Claim 6 wherein the compound is represented by the following structural formula: to.:a fe o 9 0 0 50000 and physiologically acceptable salts thereof, wherein Ri is -H or -OH.

13. The method of Claim 12 wherein n is two or three and R, is an aliphatic or substituted aliphatic group.

14. The method of Claim 3 wherein the compound is represented by the following structural formula: 0__ z (CH 2 NR 2 and physiologically acceptable salts thereof. -48- The method of Claim 14 wherein n is 3 and R, is an aromatic group, a substituted aromatic group or an aliphatic group substituted with an aromatic or substituted aromatic group.

16. The method of Claim 6 wherein the compound is represented by the following structural formula: 0 R2 and physiologically acceptable salts thereof. *&see

17. The method of Claim 16 wherein n is 3 and R 2 is an S 10 aromatic group, a substituted aromatic group or an 00 'aliphatic group substituted with an aromatic or substituted aromatic group.

18. The method of Claim 2 wherein and are each a covalent bond. O*

19. The method of Claim 18 wherein Z is represented by the following structural formula: A C B (IXa) wherein: -49- Ring C is a substituted or unsubstituted C 7 or C, non-aromatic carbocyclic ring or a substituted or unsubstituted non-aromatic heterocyclic ring and is bonded to the alkylene group by a single covalent bond between the alkylene group and a ring atom in Ring C which is not also in Ring A or Ring B. The method of Claim 18 wherein Z is represented by a structural formula selected from: A B or A B wherein: X, is a chemical bond, -CH 2 or -CHS-; W is -H or an electron withdrawing group; and ~wherein ring A and ring B are substituted or unsubstituted.

21. The method of Claim 20 wherein Z is represented by the following structural formula: wherein Ring A and/or Ring B are substituted or unsubstituted.

22. The method of Claim 21 wherein M is >NR 2 >C(OH)R, or >CHR 2

23. The method of Claim 22 wherein n is three and W is -CN.

24. The method of Claim 20 wherein Z is represented by the following structural formula: W f wherein Ring A and/or Ring B are substituted or unsubstituted. The method of Claim 24 wherein M is >C(OH)R, or >CHR,.

26. The method of Claim 25 wherein W is -CN and n is three.

27. The method of Claim 1 wherein the compound is represented by a structural formula selected from: -51- oor O N i.: NC NC OH OH and physiologically acceptable salts thereof.

28. The method of Claim 1 wherein: and are each a covalent bond; -52- Z is represented by a structural formula selected from: X1 X1 A B or A B wherein: X 1 is a chemical bond, -CH 2 or -CH 2 S-; W is -CN, alkylsulfonyl, carboxamido or carboxyalkyl; is an integer from Ring A is substituted with Re and wherein R e and R 9 are independently a halogen, alkoxy or alkyl, or, taken together with ring A, form a naphthyl group; Ra and Rb are independently alkyl, aralkyl 15 or, taken together with the nitrogen atom bonded to Ra and Rb, form a non-aromatic heterocyclic ring represented by a structure selected from: -53- 0 OH HO R 6 R N N N N R 0 S N >-an (N) N N N I i.: and physiologically acceptable salts thereof, wherein: R 5 is alkanoyl, aroyl, aralkoyl, alkyl, 5 aralkyl or cycloalkyl; R is an aryl group; and R, is -H or a heterocylic ring.

29. The method of Claim 1 wherein the compound is represented by the following structural formula: (CH 2 1 N Ra I I Rb and physiologically acceptable salts thereof. -54- The method of Claim 29; wherein: n is an integer from about 2-4; Ra is a Cl to about C4 substituted or unsubstituted alkyl group; and Rb is -(CH 2 )m-Ri wherein m is an integer from about 2-4 and R 10 is an aromatic group.

31. A method of treating a subject with a disease associated with aberrant leukocyte recruitment and/or activation comprising administering to the individual a therapeutically effective amount of a compound represented by the following structural formula: R12 N r vh B^ JQ R13 n R 11 and physiologically acceptable salts thereof, wherein: A is >NR 14 -CH 2 -CH(R 14 or (R 4 Rs) is halogen, -CN, an aliphatic group, a substituted aliphatic group, an aromatic group, a substituted aromatic group, -OH, -O-(aliphatic group), -0-(substituted aliphatic group), -S-(aliphatic groups), -S-(substituted aliphatic groups), -NO 2 -NH 2 -NH(aliphatic group), -NH(substituted aliphatic group), -N(aliphatic group) -N(substituted aliphatic group) 2; R 12 an aromatic group or an aliphatic group; each R3 is independently chosen and is an aliphatic group or a substituted aliphatic group; n is an integer from one to about four; B is -N(R 1 6 or a covalent bond; and R 14 R 15 and R, 1 are independently an aliphatic or substituted aliphatic group, and can be the same or different; Q is an aliphatic group, a substituted aliphatic group, a benzylic group, a substituted benzylic group, an aromatic group, a substituted aromatic group, a non-aromatic heterocyclic group or a substituted non- aromatic heterocyclic group; and wherein B, Q and the terminal olefin carbon, taken together, can form a substituted or 5 unsusbstituted non-aromatic heterocyclic ring which is optionally fused to a substituted or unsubstituted aromatic group.

32. The method of Claim 31 wherein: n is 1; B is -N(R 1 6 or a covalent bond; and Q is an aliphatic group, a substituted aliphatic group, a benzylic group, a substituted benzylic group, an aromatic group, a substituted aromatic group, a non-aromatic heterocyclic group or a substituted non- aromatic heterocyclic group.

33. The method of Claim 31 wherein the compound is represented by the following structural formula: -56- R 12 R1 R1 and physiologically acceptable salts thereof.

34. The method of Claim 31 wherein the compound is represented by the following structural formula: R 12 N I- and physiologically acceptable salts thereof. The method of Claim 31 wherein the compound is represented by the following structural formula: -57- R12 R 6 N+ N.S RIl and physiologically acceptable salts thereof.

36. The method of Claim 31 wherein the compound is 5 represented by the following structural formula: R12 SN and physiologically acceptable salts thereof. R o *ooo -58-

37. The method of Claim 31 wherein the compound is represented by the following structural formula: R12 N+ Q RI and physiologically acceptable salts thereof. 5 38. The method of Claim 31 wherein the compound is represented by the following structural formula: R12 1R12 NN+ *R 11 and physiologically acceptable salts thereof.

39. The method of Claim 31 wherein the compound is represented by the following structural formula: -59- and physiologically acceptable salts thereof. The method of Claim 31 wherein the compound is represented by the following structural formula: S S S and physiologically acceptable salts thereof.

41. The method of Claim 31 wherein the compound is represented by the following structural formula: R12 RR13 0 and physiologically acceptable salts thereof.

42. The method of Claim 31 wherein the compound is represented by the following structural Formula: 5 R12 o "wherein Ring D is a substituted or unsubstituted non-aromatic heterocyclic ring which is optionally fused to a substituted or unsubstituted aromatic group.

43. A method of treating a subject with a disease associated with aberrant leukocyte recruitment and/or activation comprising administering to the individual a therapeutically effective amount of a compound represented by the following structural formula: represented by the following structural formula: -61- R21' I R23 and physiologically acceptable salts thereof, wherein: R 21 is -OH, an aliphatic group, a substituted aliphatic group, -O-(aliphatic group), -O-(substituted aliphatic group), -O-CO-(aliphatic group) or -0-CO-(substituted aliphatic group; R 22 and R 23 are independently an aliphatic 10 group, a substituted aliphatic group, an aromatic .0 group, a substituted aromatic group, -S-(aliphatic group), -S-(substituted aliphatic group), -O-(aliphatic group), -O-(substituted aliphatic group), -(CH 2 26 and, taken together, can be a -(CH 2 2 to -(CH 2 5 alkylene group or a -(CH 2 2 to S-(CH 2 alkylene group substituted with one or more aliphatic groups, substituted aliphatic groups, aromatic groups or substituted aromatic groups; and R 26 is a substituted or unsubstituted aromatic 20 group.

44. The method of Claim 43 wherein: R 21 is -OH, an alkyl group, an alkoxy group, an acetoxy group or an alkyl group substituted with -N 24 R 2 5 R 2 2 and Rz 3 are independently an alkyl group, an aromatic group, an aralkyl group, and ethylene-R 2 6 or thioalkyl, and, taken together, form an alkylene group; -62- R 2 4 and R 25 are independently an alkyl group, an aralkyl group and an aryl group; R 26 is a phenyl group substituted by R 27 and R 2 s; and R 2 and R 28 are independently -OH, alkoxy, or halogen. The method of Claim 43 wherein: Rz 2 is -OH, CH 3 CO-O- or an alkyl group substituted with CH 3 NH-; R 22 is thioalkyl, alkyl or phenyl; and R 23 is methyl or, taken together with R 22 a propylene group, wherein the propylene group is unsubstituted or substituted with one or more methyl 15 or ethyl groups. :o

46. The method of Claim 44 wherein: R 21 is -OH, CH 3 CO-O- or -CH(-CH(CH 3 2 (-CH 2 NHCH3); R 22 is -SCH5, methyl or phenyl; and R 23 is methyl or, taken together with R 22 a -CH 2 CH 2 C (CH 3 2- group.

47. A method of treating a subject with a disease associated with aberrant leukocyte recruitment and/or activation comprising administering to the individual a therapeutically effective amount of a compound represented by the following structural formula: R41\+R 4 2 ON and physiologically acceptable salts thereof, wherein: -63- R 40 and R 43 are independently an aliphatic group, a substituted aliphatic group, a benzylic group, a substituted benzylic group, an aromatic group, a substituted aromatic group, a non-aromatic heterocyclic group or a substituted non-aromatic heterocyclic group; R 41 and R 42 are independently an aliphatic group or a substituted aliphatic group.

48. The method of Claim 47 wherein R, 4 and R42 are each a methyl group.

49. A method of treating a subject with a disease associated with aberrant leukocyte recruitment and/or activation comprising administering to the individual a therapeutically effective amount of a compound 15 represented by the following structural formula: R52 R 50 R R53 I51 I R51 R52 R53 wherein: Rs, and R 51 are independently -OH, a halogen, -0- (aliphatic group), -0-(substituted aliphatic group), 0-CO-(aliphatic group), -0-CO-(substituted aliphatic group), -NH 2 -NH(aliphatic group) -NH(substituted aliphatic group), -N(aliphatic group) 2 -N(substituted -64- aliphatic group) 2 -S-(aliphatic group) or -S- (substituted aliphatic group; and R 52 and R 53 are independently an aliphatic group, a substituted aliphatic group, a halogen, -NH 2 -NH(aliphatic group), -NH(substituted aliphatic group), -N(aliphatic group) 2 or -N(substituted aliphatic group)2. The method of Claim 49 wherein: R 5 o and R 51 are independently -OH, a halogen, -0- (aliphatic group) or -O-(substituted aliphatic group); and R 2 and R 53 are independently an aliphatic group, a substituted aliphatic group or a halogen.

51. Use of a compound for the manufacture of a medicament 15 for the treatment or prevention of a disease in a subject, said disease being associated with aberrant leukocyte recruitment and/or activation, and said compound being represented by the following structural formula: 20 R Z-Y--(CH X-N Rb and physiologically acceptable salts thereof, wherein: Z is a substituted or unsubstituted aromatic group; Y is a covalent bond, or -CO-; n is an integer from one to about five; X is a covalent bond or and Ra is an aliphatic or a substituted aliphatic group; and Rb is an aliphatic group substituted with an aromatic group or substituted aromatic group; and wherein Ra and Rb, taken together with the nitrogen atom bonded to R, and Rb, can form a substituted or unsubstituted non-aromatic heterocyclic ring.

52. The use of Claim 51 wherein Z is represented by a structural formula selected from: 10 X1 X A B or A B ee wherein: Xi is a chemical bond, -CHZ- or -CH2S-; W is -CN, alkylsulfonyl, carboxamido or 15 carboxyalkyl; n is an integer from Ring A is substituted with Re and R 9 wherein Re and R 9 are independently a halogen, alkoxy or alkyl, or, taken together with ring A, form a naphthyl group; Ra and Rb are independently alkyl, aralkyl or, taken together with the nitrogen atom bonded to Ra and Rb, form a non-aromatic heterocyclic ring represented by a structure selected from: -66- 0 OH HO R 6 R N N N N R and N N N N and physiologically acceptable salts thereof, wherein: R 5 is alkanoyl, aroyl, aralkoyl, alkyl, aralkyl or cycloalkyl; R 6 is an aryl group; and R, is -H or a heterocylic ring.

53. The use of Claim 51 wherein the compound is represented by a structural formula selected from: -67- or o N H NC 0 N 0. F an phsooial acetbeslsteef -68-

54. Use of a compound for the manufacture of a medicament for the treatment or prevention of a disease in a subject, said disease being associated with aberrant leukocyte recruitment and/or activation, and said compound being represented by the following structural formula: Ri2 N BQ A+ n A 13 n R11 and physiologically acceptable salts thereof, S* wherein: A is >NR14, -CH2-, -CH (R14)- or S -C(R14R15) is halogen, -CN, an aliphatic group, a substituted aliphatic group, an aromatic group, a substituted aromatic group, -OH, -O-(aliphatic group), -0-(substituted aliphatic group), -S-(aliphatic groups), -S-(substituted aliphatic groups), -NO 2 -NH 2 -NH(aliphatic group), -NH(substituted aliphatic group), -N(aliphatic group) 2 -N(substituted aliphatic group) 2 RI 2 an aromatic group or an aliphatic group; each R 13 is independently chosen and is an aliphatic group or a substituted aliphatic group; n is an integer from one to about four; B is -N(R 16 or a covalent bond; and -69- R 14 R 15 and R 16 are independently an aliphatic or substituted aliphatic group, and can be the same or different; Q is an aliphatic group, a substituted aliphatic group, a benzylic group, a substituted benzylic group, an aromatic group, a substituted aromatic group, a non-aromatic heterocyclic group or a substituted non- aromatic heterocyclic group; and wherein B, Q and the terminal olefin carbon, taken.together, can form a substituted or unsusbstituted non-aromatic heterocyclic ring which is optionally fused to a substituted or unsubstituted aromatic group.

55. Use of a compound for the manufacture of a medicament 15 for the treatment or prevention of a disease in a subject, said disease being associated with aberrant leukocyte recruitment and/or activation, and said compound being represented by the following structural formula: HO R 22 R 21 R23 and physiologically acceptable salts thereof, wherein: Rn 2 is -OH, an aliphatic group, a substituted aliphatic group, -0-(aliphatic group), -O-(substituted aliphatic group), -O-CO-(aliphatic group) or -O-CO-(substituted aliphatic group; R 2 2 and R 2 3 are independently an aliphatic group, a substituted aliphatic group, an aromatic group, a substituted aromatic group, -S-(aliphatic group), -S-(substituted aliphatic group), -0-(aliphatic group), -0-(substituted aliphatic group), -(CH 2 )n-R 26 and, taken together, can be a -(CH 2 2 to -(CH 2 5 alkylene group or a -(CH 2 2 to -(CH 2 alkylene group substituted with one or more aliphatic groups, substituted aliphatic groups, aromatic groups or substituted aromatic groups; and R 26 is a substituted or unsubstituted aromatic group.

56. The use of Claim 55 wherein: R 21 is -OH, an alkyl group, an alkoxy group, an S 15 acetoxy group or an alkyl group substituted with -NR 24 R 25 R 2 2 and R 23 are independently an alkyl group, an aromatic group, an aralkyl group, and ethylene-R 26 or thioalkyl, and, taken together, form an alkylene 20 group; R, 2 and R 25 are independently an alkyl group, an aralkyl group and an aryl group; R 26 is a phenyl group substituted by R 27 and R 28 and R 27 and R 28 are independently -OH, alkoxy, or halogen.

57. Use of a compound for the manufacture of a medicament for the treatment or prevention of a disease in a subject, said disease being associated with aberrant leukocyte recruitment and/or activation, and said compound being represented by the following structural formula: -71- R 3 R52 RoR R 0 R 50 R51 j "r R52 R53 wherein: R 50 and R 51 are independently -OH, a halogen, -0- (aliphatic group), -0-(substituted aliphatic group), O-CO-(aliphatic group), -O-CO-(substituted aliphatic Sgroup), -NH 2 -NH(aliphatic group), -NH(substituted aliphatic group), -N(aliphatic group),, -N(substituted ••aliphatic group) 2 -S-(aliphatic group) or -S- (substituted aliphatic group; and R 52 and R53 are independently an aliphatic e'g group, a substituted aliphatic group, a halogen, -NH 2 -NH(aliphatic group), -NH(substituted aliphatic group), -N(aliphatic group) 2 or -N(substituted aliphatic group) 2.

58. Use of a compound for the manufacture of a medicament for the treatment or prevention of a disease in a subject, said disease being associated with aberrant leukocyte recruitment and/or activation, and said compound being represented by the following structural formula: -72- R41\+/R42 R 4 O N R 43 and physiologically acceptable salts thereof, wherein: R 40 and R 43 are independently an aliphatic group, a substituted aliphatic group, a benzylic group, a substituted benzylic group, an aromatic group, a substituted aromatic group, a non-aromatic heterocyclic group or a substituted non-aromatic heterocyclic group; 10 R 41 and R 42 are independently an aliphatic group or a substituted aliphatic group.

59. A pharmaceutical composition comprising the compound of Claim 31 and a suitable pharmaceutical carrier. A pharmaceutical composition comprising the compound of Claim 43 and a suitable pharmaceutical carrier.

61. A pharmaceutical composition comprising the compound of Claim 44 and a suitable pharmaceutical carrier.

62. A pharmaceutical composition comprising the compound of Claim 47 and a suitable pharmaceutical carrier.

63. A pharmaceutical composition comprising the compound of Claim 49 and a suitable pharmaceutical carrier.

64. A compound for use in the treatment or prevention of a disease in a subject, said disease being associated with aberrant leukocyte recruitment and/or activation, -73- and said compound being represented by the following structural formula: R12 A R 13 R 11 and physiologically acceptable salts thereof, 5 wherein: A is >NR14, -CH -CH(R 14 or S-C (R 4 R 1 5 R 1 is halogen, -CN, an aliphatic group, a substituted aliphatic group, an aromatic group, a substituted aromatic group, -OH, -O-(aliphatic group), -O-(substituted aliphatic group), -S-(aliphatic groups), -S-(substituted aliphatic groups), -NH,, -NH(aliphatic group), -NH(substituted aliphatic group), -N(aliphatic group),, -N(substituted aliphatic group) 2 R, an aromatic group or an aliphatic group; each R 13 is independently chosen and is an aliphatic group or a substituted aliphatic group; n is an integer from one to about four; B is -N(R 16 or a covalent bond; and R 14 R 15 and R16 are independently an aliphatic or substituted aliphatic group, and'can be the same or different; Q is an aliphatic group, a substituted aliphatic group, a benzylic group, a substituted benzylic group, an aromatic group, a substituted aromatic group, a -74- non-aromatic heterocyclic group or a substituted non- aromatic heterocyclic group; and wherein B, Q and the terminal olefin carbon, taken together, can form a substituted or unsusbstituted non-aromatic heterocyclic ring which is optionally fused to a substituted or unsubstituted aromatic group. A compound for use in the treatment or prevention of a disease in a subject, said disease being associated with aberrant leukocyte recruitment and/or activation, and said compound being represented by the following structural formula: HO R22 R21 R 23 and physiologically acceptable salts thereof, wherein: R 2 is -OH, an aliphatic group, a substituted aliphatic group, -O-(aliphatic group), -0-(substituted aliphatic group), -0-CO-(aliphatic group) or -O-CO-(substituted aliphatic group; R 22 and R 2 3 are independently an aliphatic group, a substituted aliphatic group, an aromatic group, a substituted aromatic group, -S-(aliphatic group), -S-(substituted aliphatic group), -0-(aliphatic group), -O-(substituted aliphatic group), -(CH 2 )n-R 26 and, taken together, can be a -(CH 2 2 to -(CH 2 5 alkylene group or a -(CH 2 2 to 5 alkylene group substituted with one or more aliphatic groups, substituted aliphatic groups, aromatic groups or substituted aromatic groups; and R 26 is a substituted or unsubstituted aromatic group.

66. The compound of Claim 65 wherein: R 21 is -OH, an alkyl group, an alkoxy group, an acetoxy group or an alkyl group substituted with -NR 24 R 25 R 22 and R 23 are independently an alkyl group, an aromatic group, an aralkyl group, and ethylene-R 6 or thioalkyl, and, taken together, form an alkylene group; R 24 and R 25 are independently an alkyl group, an aralkyl group and an aryl group; 15 R 26 is a phenyl group substituted by R, 7 and Rz 2 and R 27 and R 28 are independently -OH, alkoxy, or halogen.

67. A compound for use in the treatment or prevention of a 20 disease in a subject, said disease being associated with aberrant leukocyte recruitment and/or activation, and said compound being represented by the following structural formula: *o* -76- wherein: R 50 and R 51 are independently -OH, a halogen, -0- (aliphatic group), -0-(substituted aliphatic group), 5 O-CO-(aliphatic group), -O-CO-(substituted aliphatic group), -NH 2 -NH(aliphatic group), -NH(substituted aliphatic group), -N(aliphatic group),, -N(substituted aliphatic group) 2 -S-(aliphatic group) or -S- (substituted aliphatic group; and 10 R 52 and R 53 are independently an aliphatic group, a substituted aliphatic group, a halogen, -NH 2 -NH(aliphatic group), -NH(substituted aliphatic group), -N(aliphatic group) 2 or -N(substituted aliphatic group)2*

68. A compound for use in the treatment or prevention of a disease in a subject, said disease being associated with aberrant leukocyte recruitment and/or activation, and said compound being represented by the following structural formula: R41\+ R 4 2 N -77- and physiologically acceptable salts thereof, wherein: R 40 and R 43 are independently an aliphatic group, a substituted aliphatic group, a benzylic group, a substituted benzylic group, an aromatic group, a substituted aromatic group, a non-aromatic heterocyclic group or a substituted non-aromatic heterocyclic group; R 41 and R 42 are independently an aliphatic group or a substituted aliphatic group. DATED this 1st day of May, 2001 Millenium Pharmaceuticals, Inc. By DAVIES COLLISON CAVE Patent Attorneys for the Applicant *ee o*e* gog