AU2014236763B2 - Thioesterases and cells for production of tailored oils - Google Patents

Abstract

The invention features plant acyl-ACP thioesterase genes of the FatB class and proteins encoded by these genes. The genes are useful for constructing recombinant host cells having altered fatty acid profiles. Oleaginous microalga host cells with the new genes or previously identified FatB genes are disclosed. The microalgae cells produce triglycerides with useful fatty acid profiles.

Description

THIOESTERASES AND CELLS FOR PRODUCTION OF TAILORED OILS Cross Reference to related Applications [0001] This application is a Continuation-in-part of United States Patent 5 Application No. 13/837,996, filed March 15, 2013, and claims the benefit of United States Provisional Patent Application Serial No. 61/791,861, filed March 15, 2013, and United States Provisional Patent Application Serial No. 61/917,217, filed December 17, 2013, each of which is hereby incorporated by reference herein in its entirety. 10 Background [0002] Certain organisms including plants and some microalgae use a type II fatty acid biosynthetic pathway, characterized by the use of discrete, monofunctional enzymes for fatty acid synthesis. In contrast, mammals and fungi use a single, large, multifunctional protein. 15 [0003] Type II fatty acid biosynthesis typically involves extension of a growing acyl-ACP (acyl-carrier protein) chain by two carbon units followed by cleavage by an acyl-ACP thioesterase. In plants, two main classes of acyl-ACP thioesterases have been identified: (i) those encoded by genes of the FatA class, which tend to hydrolyze oleoyl-ACP into oleate (an 18:1 fatty acid) and ACP, and (ii) 20 those encoded by genes of the FatB class, which liberate C8-C16 fatty acids from corresponding acyl-ACP molecules.

[0004] Different FatB genes from various plants have specificities for different acyl chain lengths. As a result, different gene products will produce different fatty acid profiles in plant seeds. See, US Patent Nos. 5,850,022; 5,723,761; 25 5,639,790; 5,807,893; 5,455,167; 5,654,495; 5,512,482;5,298,421;5,667,997; and 5,344,771; 5,304,481. Recently, FatB genes have been cloned into oleaginous microalgae to produce triglycerides with altered fatty acid profiles. See, WO2010/063032, WO2011/150411, W02012/106560, and WO2013/158938.

Summary [0005] In various aspects, the invention(s) contemplated herein may include, but need not be limited to, any one or more of the following embodiments: [0006] Embodiment 1: A nucleic acid construct including a regulatory 5 element and a FatB gene expressing an active acyl-ACP thioesterase operable to produce an altered fatty acid profile in an oil produced by a cell expressing the nucleic acid construct, wherein the FatB gene expresses a protein having an amino acid sequence falling within clade 5 of Table la, the sequence having at least 94.6% sequence identity with each of SEQ ID NOs: 88, 82, 85, and 103, and optionally 10 wherein the fatty acid of the oil is enriched in C8 and CIO fatty acids.

[0007] Embodiment 2: A nucleic acid construct including a regulatory element and a FatB gene expressing an active acyl-ACP thioesterase operable to produce an altered fatty acid profile in an oil produced by a cell expressing the nucleic acid construct, wherein the FatB gene expresses a protein having an amino acid 15 sequence falling within one of clades 1-12 of Table la.

[0008] Embodiment 3: The nucleic acid construct of embodiment 2, wherein the FatB gene expresses a protein having an amino acid sequence falling within clade 1 of Table la, the sequence having at least 85.9% sequence identity with each of SEQ ID NOs: 19, 161, 22, and 160, and optionally wherein the fatty acid of the oil is 20 enriched in C14 and C16 fatty acids.

[0009] Embodiment 4: The nucleic acid construct of embodiment 2, wherein the FatB gene expresses a protein having an amino acid sequence falling within clade 2 of Table la, the sequence having at least 89.5% sequence identity with each of SEQ ID NOs: 134-136, 132, 133, 137, 124, 122, 123, 125, and optionally wherein the fatty 25 acid of the oil is enriched in C12 and C14 fatty acids.

[0010] Embodiment 5: The nucleic acid construct of embodiment 2, wherein the FatB gene expresses a protein having an amino acid sequence falling within clade 3 of Table la, the sequence having at least 92.5% sequence identity with each of SEQ ID NOs: 126 and 127, and optionally wherein the fatty acid of the oil is enriched in 30 C12 and C14 fatty acids.

[0011] Embodiment 6: The nucleic acid construct of embodiment 2, wherein the FatB gene expresses a protein having an amino acid sequence falling within clade 4 of Table la, the sequence having at least 83.8% sequence identity with SEQ ID NO: 79, and optionally wherein the fatty acid of the oil is enriched in C12 and C14 fatty acids.

[0012] Embodiment 7: The nucleic acid construct of embodiment 2, wherein 5 the FatB gene expresses a protein having an amino acid sequence falling within clade 6 of Table la, the sequence having at least 99.9% sequence identity with each of SEQ ID NOs: 111 and 110, and optionally wherein the fatty acid of the oil is enriched in CIO fatty acids.

[0013] Embodiment 8: The nucleic acid construct of embodiment 2, wherein 10 the FatB gene expresses a protein having an amino acid sequence falling within clade 7 of Table la, the sequence having at least 89.5% sequence identity with each of SEQ ID NOs: 73, 106, 185, 172, 171, 173, 174, and optionally wherein the fatty acid of the oil is enriched in CIO and C12 fatty acids.

[0014] Embodiment 9: The nucleic acid construct of embodiment 2, wherein 15 the FatB gene expresses a protein having an amino acid sequence falling within clade 8 of Table la, the sequence having at least 85.9% sequence identity with each of SEQ ID NOs: 112, 113, 142, 145, 143, 144, 139, 140, 138, 141, and optionally wherein the fatty acid of the oil is enriched in C12 and C14 fatty acids.

[0015] Embodiment 10: The nucleic acid construct of embodiment 2, 20 wherein the FatB gene expresses a protein having an amino acid sequence falling within clade 9 of Table la, the sequence having at least 83.8% sequence identity with each of SEQ ID NOs: 187-189, and optionally wherein the fatty acid of the oil is enriched in C12 and C14 fatty acids.

[0016] Embodiment 11: The nucleic acid construct of embodiment 2, 25 wherein the FatB gene expresses a protein having an amino acid sequence falling within clade 10 of Table la, the sequence having at least 95.9% sequence identity with each of SEQ ID NOs: 147, 149, 146, 150, 152, 151, 148, 154, 156, 155, 157, 108, 75, 190, 191, and 192, and optionally wherein the fatty acid of the oil is enriched in C14 and C16 fatty acids. 30 [0017] Embodiment 12: The nucleic acid construct of embodiment 2, wherein the FatB gene expresses a protein having an amino acid sequence falling within clade 11 of Table la, the sequence having at least 88.7% sequence identity with SEQ ID NO: 121, and optionally wherein the fatty acid of the oil is enriched in C14 and C16 fatty acids.

[0018] Embodiment 13: The nucleic acid construct of embodiment 2, wherein the FatB gene expresses a protein having an amino acid sequence falling within clade 12 of Table 5 1 a, the sequence having at least 72.8% sequence identity with each of SEQ ID NOs: 129 and 186, and optionally wherein the fatty acid of the oil is enriched in C16 fatty acids.

[0019] Embodiment 14: An isolated nucleic acid or recombinant DNA construct including a nucleic acid, wherein the nucleic acid has at least 80% sequence identity to any of SEQ ID NOS: 2,3, 5, 6, 8, 9,11,12,14,15, 17,18,20,21,23, 24,26, 27, 29, 30,32, 33, 35, 10 36,38,39,41,42,44,45,47,48,50,51, 53, 54,56,57,59,60,62,63,65, 66,68,69, 71,72, 74, 76, 78, 80, 81, 83, 84, 86, 87, 89, 90, 92, 93, 95, 96, 98, 99,101, 102,104,105, 107,109 or any equivalent sequences by virtue of the degeneracy of the genetic code.

[0020] Embodiment 15: An isolated nucleic acid sequence encoding a protein or a host cell expressing a protein having at least 80% sequence identity to any of SEQ ID NOS: 1, 15 4, 7,10,13,16,19,22,25,28,31, 34,37,40,43,46,49,52,55,58,61,64,67,70,73,75,77,

79, 82, 85, 88,91,94,97,100,103,106,108,110-192 or a fragment thereof having acyl-ACP thioesterase activity.

[0021] Embodiment 16: The isolated nucleic acid of embodiment 15, wherein, the protein has acyl-ACP thioesterase activity operable to alter the fatty acid profile of an oil 20 produced by a recombinant cell including that sequence.

[0021a] Embodiment 17: A recombinant nucleic acid encoding an acyl-ACP thioesterase, wherein the nucleic acid has at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, or 99% sequence identity to SEQ ID NO 93, and wherein the nucleic acid encodes an acyl-ACP thioesterase having at least 96% sequence identity to SEQ ID NOs: 91, 171 or 179, or 25 a C-terminal and/or N-terminal truncated fragment comprising at least 90% of the full-length sequences thereof.

[0021b] Embodiment 18: A recombinant nucleic acid encoding an acyl-ACP thioesterase, wherein the nucleic acid has at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, or 99% sequence identity to any of SEQ ID NO: 90, and wherein the nucleic acid encodes 30 an acyl-ACP thioesterase of any one of SEQ ID NOs: 88, 170 or 178, or a C-terminal and/or N-terminal truncated fragment comprising at least 90% of the full-length sequences thereof.

[0021c] Embodiment 19: A recombinant nucleic acid encoding an acyl-ACP thioesterase, wherein the nucleic acid has at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, 5 or 99% sequence identity to any of SEQ ID NO: 84, and wherein the nucleic acid encodes an acyl-ACP thioesterase having at least 95% sequence identity to SEQ ID NO:82, or a C-terminal and/or N-terminal truncated fragment comprising at least 90% of the full-length sequences thereof.

[0021d] Embodiment 20: A vector comprising the recombinant nucleic acid of any 10 one of embodiments 17 to 19.

[0022] Embodiment 21: A method of producing a recombinant cell that produces an altered fatty acid profile, the method including transforming the cell with a nucleic acid according to any of embodiments 1-3, or 17-19.

[0023] Embodiment 22: A host cell produced by the method of embodiment 21. 15 [0024] Embodiment 23: The host cell of embodiment 22, wherein the host cell is selected from a plant cell, a microbial cell, and a microalgal cell.

[0024a] Embodiment 24: A method of producing a recombinant acyl-ACP thioesterase, the method comprising transforming a Prototheca microalgal cell with a nucleic acid according to any one of embodiments 17-19.

20 [0024b] Embodiment 25: A method of producing a recombinant acyl-ACP thioesterase, the method comprising transforming a Prototheca microalgal cell with a nucleic acid encoding an acyl-ACP thioesterase: a) having at least 96% sequence identity to SEQ ID NOs: 91, 171 or 179; b) of any one of SEQ ID NOs:88, 170 or 178; or 25 c) having at least 95% sequence identity to SEQ ID NO:82; or d) a C-terminal and/or N-terminal truncated fragment of a)-c) comprising at least 90% of the full-length sequences thereof.

[0024c] Embodiment 26: A Prototheca microalgal host cell comprising a recombinant nucleic acid of any one of embodiments 17-19, wherein the recombinant 30 nucleic acid comprises an exogenous nucleic acid, and wherein the host cell has a fatty acid profile having increased C8 and/or CIO fatty acids. 10024(11 Embodiment 27: A Prototheca microalgal host cell comprising a recombinant nucleic acid encoding an acyl-ACP thioesterase: a) having at least 96% sequence identity to SEQ ID NOs: 91, 171 or 179; b) of any one of SEQ ID NOs:88, 170 or 178; or 5 c) having at least 95% sequence identity to SEQ ID NO:82; or d) a C-terminal and/or N-terminal truncated fragment of a)-c) comprising at least 90% of the full-length sequences thereof, and wherein the host cell has a fatty acid profile having increased C8 and/or CIO fatty acids.

[0025] Embodiment 28: A method for producing an oil or oil-derived product, the 10 method including cultivating a host cell of embodiment 21, 22 or 26, and extracting oil produced thereby, optionally wherein the cultivation is heterotrophic growth on sugar.

[0026] Embodiment 29: The method of embodiment 28, further including producing a fatty acid, fuel, chemical, or other oil-derived product from the oil.

[0027] Embodiment 30: An oil produced by the method of embodiment 28, 15 optionally having a fatty acid profile including at least about 8% C8 and/or at least about 9% CIO fatty acids.

[0028] Embodiment 31: An oil-derived product produced by the method of embodiment 29.

[0029] Embodiment 32: The oil-derived product of embodiment 31, wherein the 20 oil is produced by a microalgae and optionally, lacks C24-alpha sterols.

[0029a] Embodiment 33: The oil-derived product of embodiment 32, wherein the oil comprises sterols with C24f:S stereochemistry.

[0029b] Embodiment 34: The oil-derived product of embodiment 32 or 33, wherein the oil comprises one or more of ergosterol, brassicasterol, 22, 23-dihydrobrassicasterol, 25 clionasterol and poriferasterol.

[0029c] Embodiment 35: The oil-derived product of any one of embodiments 32-34, wherein the oil comprises one or more of ergosterol, brassicasterol, 22, 23-dihydrobrassicasterol, clionasterol and poriferasterol.

[0029d] The reference in this specification to any prior publication (or information 30 derived from it), or to any matter which is known, is not, and should not be taken as an acknowledgment or admission or any form of suggestion that that prior publication (or information derived from it) or known matter forms part of the common general knowledge in the field of endeavour to which this specification relates.

[0029e] Throughout this specification and the claims which follow, unless the context requires otherwise, the word “comprise”, and variations such as “comprises” and 5 “comprising”, will be understood to imply the inclusion of a stated integer or step or group of integers or steps but not the exclusion of any other integer or step or group of integers or steps.

Description of Illustrative Embodiments of the Invention 10 Definitions [0030] As used with respect to nucleic acids, the term “isolated” refers to a nucleic acid that is free of at least one other component that is typically present with the naturally occurring nucleic acid. Thus, a naturally occurring nucleic acid is isolated if it has been purified away from at least one other component that occurs naturally with the nucleic 15 acid.

[0031] A “natural oil” or “natural fat” shall mean a predominantly triglyceride oil obtained from an organism, where the oil has not undergone blending with another natural or synthetic oil, or fractionation so as to substantially alter the fatty acid profile of the triglyceride. In connection with an oil comprising triglycerides of a particular 20 regiospecificity, the natural oil or natural fat has not been subjected to interesterification or other synthetic process to obtain that regiospecific triglyceride profile, rather the regiospecificity is produced naturally, by a cell or population of cells. In connection with a natural oil or natural fat, and as used generally throughout the present disclosure, the terms oil and fat are used interchangeably, except where otherwise noted. Thus, an “oil” 25 or a “fat” can be liquid, solid, or partially solid at room temperature, depending on the makeup of the substance and other conditions. Here, the term “fractionation” means removing material from the oil in a way that changes its fatty acid profile relative to the profile produced by the organism, however accomplished. The terms “natural oil” and “natural fat” encompass such oils obtained from an organism, where the oil has undergone minimal processing, including refining, bleaching and/or degumming, which does not substantially change its 5 triglyceride profile. A natural oil can also be a “noninteresterified natural oil”, which means that the natural oil has not undergone a process in which fatty acids have been redistributed in their acyl linkages to glycerol and remain essentially in the same configuration as when recovered from the organism.

[0032] “Exogenous gene” shall mean a nucleic acid that codes for the 10 expression of an RNA and/or protein that has been introduced into a cell (e.g. by transformation/transfection), and is also referred to as a “transgene”. A cell comprising an exogenous gene may be referred to as a recombinant cell, into which additional exogenous gene(s) may be introduced. The exogenous gene may be from a different species (and so heterologous), or from the same species (and so 15 homologous), relative to the cell being transformed. Thus, an exogenous gene can include a homologous gene that occupies a different location in the genome of the cell or is under different control, relative to the endogenous copy of the gene. An exogenous gene may be present in more than one copy in the cell. An exogenous gene may be maintained in a cell, for example, as an insertion into the genome 20 (nuclear or plastid) or as an episomal molecule.

[0033] “Fatty acids” shall mean free fatty acids, fatty acid salts, or fatty acyl moieties in a glycerolipid. It will be understood that fatty acyl groups of glycerolipids can be described in terms of the carboxylic acid or anion of a carboxylic acid that is produced when the triglyceride is hydrolyzed or saponified. 25 [0034] “Microalgae” are microbial organisms that contain a chloroplast or other plastid, and optionally that are capable of performing photosynthesis, or a prokaryotic microbial organism capable of performing photosynthesis. Microalgae include obligate photoautotrophs, which cannot metabolize a fixed carbon source as energy, as well as heterotrophs, which can live solely off of a fixed carbon source. 30 Microalgae include unicellular organisms that separate from sister cells shortly after cell division, such as Chlamydomonas, as well as microbes such as, for example, Volvox, which is a simple multicellular photosynthetic microbe of two distinct cell types. Microalgae include cells such as Chlorella, Dunaliella, and Prototheca.

Microalgae also include other microbial photosynthetic organisms that exhibit cellcell adhesion, such as Agmenellum, Anabaena, and Pyrobotrys. Microalgae also include obligate heterotrophic microorganisms that have lost the ability to perform photosynthesis, such as certain dinoflagellate algae species and species of the genus 5 Prototheca.

[0035] An “oleaginous” cell is a cell capable of producing at least 20% lipid by dry cell weight, naturally or through recombinant or classical strain improvement. An “oleaginous microbe” or “oleaginous microorganism” is a microbe, including a microalga that is oleaginous. 10 [0036] The term “percent sequence identity,” in the context of two or more amino acid or nucleic acid sequences, refers to two or more sequences or subsequences that are the same or have a specified percentage of amino acid residues or nucleotides that are the same, when compared and aligned for maximum correspondence, as measured using a sequence comparison algorithm or by visual 15 inspection. For sequence comparison to determine percent nucleotide or amino acid identity, typically one sequence acts as a reference sequence, to which test sequences are compared. When using a sequence comparison algorithm, test and reference sequences are input into a computer, subsequence coordinates are designated, if necessary, and sequence algorithm program parameters are designated. The sequence 20 comparison algorithm then calculates the percent sequence identity for the test sequence(s) relative to the reference sequence, based on the designated program parameters. Optimal alignment of sequences for comparison can be conducted using the NCBI BLAST software (ncbi.nlm.nih.gov/BLAST/) set to default parameters. For example, to compare two nucleic acid sequences, one may use blastn with the 25 "BLAST 2 Sequences" tool Version 2.0.12 (Apr. 21, 2000) set at the following default parameters: Matrix: BLOSUM62; Reward for match: 1; Penalty for mismatch: -2; Open Gap: 5 and Extension Gap: 2 penalties; Gap x drop-off: 50; Expect: 10; Word Size: 11; Filter: on. For a pairwise comparison of two amino acid sequences, one may use the “BLAST 2 Sequences” tool Version 2.0.12 (Apr. 21, 30 2000) with blastp set, for example, at the following default parameters: Matrix: BLOSUM62; Open Gap: 11 and Extension Gap: 1 penalties; Gap x drop-off 50; Expect: 10; Word Size: 3; Filter: on.

[0037] In connection with a natural oil, a “profile” is the distribution of particular species or triglycerides or fatty acyl groups within the oil. A “fatty acid profile” is the distribution of fatty acyl groups in the triglycerides of the oil without reference to attachment to a glycerol backbone. Fatty acid profiles are typically 5 determined by conversion to a fatty acid methyl ester (FAME), followed by gas chromatography (GC) analysis with flame ionization detection (FID). The fatty acid profile can be expressed as one or more percent of a fatty acid in the total fatty acid signal determined from the area under the curve for that fatty acid. FAME-GC-FID measurement approximate weight percentages of the fatty acids. 10 [0038] As used herein, an oil is said to be “enriched” in one or more particular fatty acids if there is at least a 10% increase in the mass of that fatty acid in the oil relative to the non-enriched oil. For example, in the case of a cell expressing a heterologous FatB gene described herein, the oil produced by the cell is said to be enriched in, e.g., C8 and C16 fatty acids if the mass of these fatty acids in the oil is at 15 least 10% greater than in oil produced by a cell of the same type that does not express the heterologous FatB gene (e.g., wild type oil).

[0039] ‘Recombinant” is a cell, nucleic acid, protein or vector that has been modified due to the introduction of an exogenous nucleic acid or the alteration of a native nucleic acid. Thus, e.g., recombinant (host) cells can express genes that are not 20 found within the native (non-recombinant) form of the cell or express native genes differently than those genes are expressed by a non-recombinant cell. Recombinant cells can, without limitation, include recombinant nucleic acids that encode a gene product or suppression elements such as mutations, knockouts, antisense, interfering RNA (RNAi) or dsRNA that reduce the levels of active gene product in a cell. A 25 “recombinant nucleic acid” is a nucleic acid originally formed in vitro, in general, by the manipulation of nucleic acid, e.g., using polymerases, ligases, exonucleases, and endonucleases, using chemical synthesis, or otherwise is in a form not normally found in nature. Recombinant nucleic acids may be produced, for example, to place two or more nucleic acids in operable linkage. Thus, an isolated nucleic acid or an 30 expression vector formed in vitro by nucleic by ligating DNA molecules that are not normally joined in nature, are both considered recombinant for the purposes of this invention. Recombinant nucleic acids can also be produced in other ways; e.g., using chemical DNA synthesis. Once a recombinant nucleic acid is made and introduced into a host cell or organism, it may replicate using the in vivo cellular machinery of the host cell; however, such nucleic acids, once produced recombinantly, although subsequently replicated intracellularly, are still considered recombinant for purposes of this invention. Similarly, a “recombinant protein” is a protein made using 5 recombinant techniques, i.e., through the expression of a recombinant nucleic acid.

[0040] Embodiments of the present invention relate to the use of FatB genes isolated from plants, which can be expressed in a host cell in order to alter the fatty acid profile of an oil produced by the recombinant cell. Although the microalga, Prototheca moriformis, was used to screen the genes for ability to the alter fatty acid 10 profile, the genes are useful in a wide variety of host cells. For example, the genes can be expressed in bacteria, other microalgae, or higher plants. The genes can be expressed in higher plants according to the methods of US Patent Nos. 5,850,022; 5,723,761; 5,639,790; 5,807,893; 5,455,167; 5,654,495; 5,512,482;5,298,421;5,667,997; 5,344,771; and 5,304,481. The fatty acids can be 15 further converted to triglycerides, fatty aldehydes, fatty alcohols and other oleochemicals either synthetically or biosynthetically.

[0041] In specific embodiments, triglycerides are produced by a host cell expressing a novel FatB gene. A triglyceride-containing natural oil can be recovered from the host cell. The natural oil can be refined, degummed, bleached and/or 20 deodorized. The oil, in its natural or processed form, can be used for foods, chemicals, fuels, cosmetics, plastics, and other uses. In other embodiments, the FatB gene may not be novel, but the expression of the gene in a microalga is novel.

[0042] The genes can be used in a variety of genetic constructs including plasmids or other vectors for expression or recombination in a host cell. The genes 25 can be codon optimized for expression in a target host cell. The proteins produced by the genes can be used in vivo or in purified form.

[0043] For example, the gene can be prepared in an expression vector comprising an operably linked promoter and 5’UTR. Where a plastidic cell is used as the host, a suitably active plastid targeting peptide can be fused to the FATB gene, 30 as in the examples below. Generally, for the newly identified FATB genes, there are roughly 50 amino acids at the N-terminal that constitute a plastid transit peptide, which are responsible for transporting the enzyme to the chloroplast. In the examples below, this transit peptide is replaced with a 38 amino acid sequence that is effective in the Prototheca moriformis host cell for transporting the enzyme to the plastids of those cells. Thus, the invention contemplates deletions and fusion proteins in order to optimize enzyme activity in a given host cell. For example, a transit peptide from the host or related species may be used instead of that of the newly discovered plant 5 genes described here.

[0044] A selectable marker gene may be included in the vector to assist in isolating a transformed cell. Examples of selectable markers useful in microlagae include sucrose invertase and antibiotic resistance genes.

[0045] The gene sequences disclosed can also be used to prepare antisense, or 10 inhibitory RNA (e.g., RNAi or hairpin RNA) to inhibit complementary genes in a plant or other organism.

[0046] FatB genes found to be useful in producing desired fatty acid profiles in a cell are summarized below in Table 1. Nucleic acids or proteins having the sequence of SEQ ID NOS: 1-109 can be used to alter the fatty acid profile of a 15 recombinant cell. Variant nucleic acids can also be used; e.g., variants having at least 70, 80, 85, 90, 95, 96, 97, 98, or 99% sequence identity to SEQ ID NOS: 2, 3, 5, 6, 8, 9, 11, 12, 14, 15, 17, 18, 20, 21, 23, 24, 26, 27, 29, 30, 32, 33, 35, 36, 38, 39, 41, 42, 44, 45, 47, 48, 50, 51, 53, 54, 56, 57, 59, 60, 62, 63, 65, 66, 68, 69, 71, 72, 74, 76, 78, 80, 81, 83, 84, 86, 87, 89, 90, 92, 93, 95, 96, 98, 99, 101, 102, 104, 105, 107 or 109. 20 Codon optimization of the genes for a variety of host organisms is contemplated, as is the use of gene fragments. Preferred codons for Prototheca strains and for Chlorella protothecoides are shown below in Tables 2 and 3, respectively. Codon usage for Cuphea wrightii is shown in Table 3a. Codon usage for Arabidopsis is shown in Table 3b; for example, the most preferred of codon for each amino acid can be 25 selected. Codon tables for other organisms including microalgae and higher plants are known in the art. In some embodiments, the first and/or second most preferred Prototheca codons are employed for codon optimization. In specific embodiments, the novel amino acid sequences contained in the sequence listings below are converted into nucleic acid sequences according to the most preferred codon usage in 30 Prototheca, Chlorella, Cuphea wrightii, or Arabidopsis as set forth in tables 2 through 3b or nucleic acid sequences having at least 70, 80, 85, 90, 95, 96, 97, 98, or 99% sequence identity to these derived nucleic acid sequences.

[0047] In embodiments of the invention, there is protein or a nucleic acid encoding a protein having any of SEQ ID NOS: 1,4, 7, 10, 13, 16, 19,22,25,28,31, 34, 37, 40, 43, 46, 49, 52, 55, 58, 61, 64, 67, 70, 73, 75, 77, 79, 82, 85, 88, 91, 94, 97, 100, 103, 106, 108, or 110-192. In an embodiment, there is protein or a nucleic acid 5 encoding a protein having at least 70, 75, 80, 85, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, or 100% sequence identity with any of SEQ ID NOS: 1, 4, 7, 10, 13, 16, 19, 22, 25, 28, 31, 34, 37, 40, 43, 46, 49, 52, 55, 58, 61, 64, 67, 70, 73, 75, 77, 79, 82, 85, 88, 91, 94, 97, 100, 103, 106, 108, or 110-192. In certain embodiments, the invention encompasses a fragment any of the above-described proteins or nucleic acids 10 (including fragments of protein or nucleic acid variants), wherein the protein fragment has acyl-ACP thioesterase activity or the nucleic acid fragment encodes such a protein fragment. In other embodiments, the fragment includes a domain of an acyl-ACP thioesterase that mediates a particular function, e.g., a specificity-determining domain. Illustrative fragments can be produced by C-terminal and/or N-terminal truncations 15 and include at least 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98%, or 99% of the full-length sequences disclosed herein.

[0048] In certain embodiments, percent sequence identity for variants of the nucleic acids or proteins discussed above can be calculated by using the full-length nucleic acid sequence (e.g., one of SEQ ID NOS: 2, 3, 5, 6, 8, 9, 11, 12, 14, 15, 17, 20 18, 20, 21, 23, 24, 26, 27, 29, 30, 32, 33, 35, 36, 38, 39, 41, 42, 44, 45, 47, 48, 50, 51, 53, 54, 56, 57, 59, 60, 62, 63, 65, 66, 68, 69, 71, 72, 74, 76, 78, 80, 81, 83, 84, 86, 87, 89, 90, 92, 93, 95, 96, 98, 99, 101, 102, 104, 105, 107 or 109) or full-length amino acid sequence (e.g., one of SEQ ID NOS: 1, 4, 7, 10, 13, 16, 19, 22, 25, 28, 31, 34, 37, 40, 43, 46, 49, 52, 55, 58, 61, 64, 67, 70, 73, 75, 77, 79, 82, 85, 88, 91, 94, 97, 100, 25 103, 106, 108, or 110-192) as the reference sequence and comparing the full-length test sequence to this reference sequence. In some embodiments relating to fragments, percent sequence identity for variants of nucleic acid or protein fragments can be calculated over the entire length of the fragment.

[0049] The nucleic acids can be in isolated form, or part of a vector or other 30 construct, chromosome or host cell. It has been found that is many cases the full length gene (and protein) is not needed; for example, deletion of some or all of the N-terminal hydrophobic domain (typically an 18 amino acid domain starting with LPDW) yields a still-functional gene. In addition, fusions of the specificity determining regions of the genes in Table 1 with catalytic domains of other acyl-ACP thioesterases can yield functional genes. Thus, in certain embodiments, the invention encompasses functional fragments (e.g., specificity determining regions) of the disclosed nucleic acid or amino acids fused to heterologous acyl-ACP thioesterase 5 nucleic acid or amino acid sequences, respectively.

Table 1: FatB genes according to embodiments of the present invention

In certain embodiments, a host cell (e.g. plant or microalgal cell) is transformed to produce a recombinant FATB protein falling into one of clades 1-12 of Table la. These clades were determined by sequence alignment and observation of changes in 5 fatty acid profile when expressed in Prototheca. See Example 5. The FATB amino acid sequence can fall within x% amino acid sequence identity of each sequence in that clade listed in Table la, where x is a first second or third cutoff value, also listed in Table la.

Table la: Groupings of Novel FatB genes into clades.

Host Cells [0050] The host cell can be a single cell (e.g., microalga, bacteria, yeast) or part of a multicellular organism such as a plant or fungus. Methods for expressing Fatb genes in a plant are given in 5,850,022; 5,723,761; 5,639,790; 5,807,893; 5,455,167; 5,654,495; 5,512,482;5,298,421;5,667,997; and 5,344,771; 5,304,481, or can be accomplished using other techniques generally known in plant biotechnology. Engineering of oleaginous microbes including those of Chlorophyta is disclosed in WO2010/063032, WO2011,150411, and W02012/106560 and in the examples below.

[0051] Examples of oleaginous host cells include plant cells and microbial cells having a type II fatty acid biosynthetic pathway, including plastidic oleaginous cells such as those of oleaginous algae. Specific examples of microalgal cells include heterotrophic or obligate heterotrophic microalgae of the phylum Chlorophtya, the class Trebouxiophytae, the order Chlorellales, or the family Chlorellacae. Examples of oleaginous microalgae are provided in Published PCT Patent Applications W02008/151149, W02010/06032, WO2011/150410, and WO2011/150411, including species of Chlorella and Prototheca, a genus comprising obligate heterotrophs. The oleaginous cells can be, for example, capable of producing 25, 30, 40, 50, 60, 70, 80, 85, or about 90% oil by cell weight, ±5%. Optionally, the oils produced can be low in DHA or EPA fatty acids. For example, the oils can comprise less than 5%, 2 %, or 1% DHA and/or EPA. The above-mentioned publications also disclose methods for cultivating such cells and extracting oil, especially from microalgal cells; such methods are applicable to the cells disclosed herein and incorporated by reference for these teachings. When microalgal cells are used they can be cultivated autotrophically (unless an obligate heterotroph) or in the dark using a sugar (e.g., glucose, fructose and/or sucrose). In any of the embodiments described herein, the cells can be heterotrophic cells comprising an exogenous invertase gene so as to allow the cells to produce oil from a sucrose feedstock. Alternately, or in addition, the cells can metabolize xylose from cellulosic feedstocks. For example, the cells can be genetically engineered to express one or more xylose metabolism genes such as those encoding an active xylose transporter, a xylulose-5-phosphate transporter, a xylose isomerase, a xylulokinase, a xylitol dehydrogenase and a xylose reductase. See WO2012/154626, “GENETICALLY ENGINEERED MICROORGANISMS THAT METABOLIZE XYLOSE”, published Nov 15, 2012.

Oils and Related Products [0052] The oleaginous cells express one or more exogenous genes encoding fatty acid biosynthesis enzymes. As a result, some embodiments feature natural oils that were not obtainable from a non-plant or non-seed oil, or not obtainable at all.

[0053] The oleaginous cells produce a storage oil, which is primarily triacylglyceride and may be stored in storage bodies of the cell. A raw oil may be obtained from the cells by disrupting the cells and isolating the oil. W02008/151149, W02010/06032, WO2011/150410, and WO2011/1504 disclose heterotrophic cultivation and oil isolation techniques. For example, oil may be obtained by cultivating, drying and pressing the cells. The oils produced may be refined, bleached and deodorized (RBD) as known in the art or as described in W02010/120939. The raw or RBD oils may be used in a variety of food, chemical, and industrial products or processes. After recovery of the oil, a valuable residual biomass remains. Uses for the residual biomass include the production of paper, plastics, absorbents, adsorbents, as animal feed, for human nutrition, or for fertilizer.

[0054] Where a fatty acid profile of a triglyceride (also referred to as a “triacylglyceride” or “TAG”) cell oil is given here, it will be understood that this refers to a nonfractionated sample of the storage oil extracted from the cell analyzed under conditions in which phospholipids have been removed or with an analysis method that is substantially insensitive to the fatty acids of the phospholipids (e.g. using chromatography and mass spectrometry). The oil may be subjected to an RBD process to remove phospholipids, free fatty acids and odors yet have only minor or negligible changes to the fatty acid profile of the triglycerides in the oil. Because the cells are oleaginous, in some cases the storage oil will constitute the bulk of all the TAGs in the cell.

[0055] The stable carbon isotope value 813C is an expression of the ratio of 13C/12C relative to a standard (e.g. PDB, carbonite of fossil skeleton of Belemnite americana from Peedee formation of South Carolina). The stable carbon isotope value 613C (0/00) of the oils can be related to the 613C value of the feedstock used.

In some embodiments, the oils are derived from oleaginous organisms heterotrophically grown on sugar derived from a C4 plant such as com or sugarcane. In some embodiments the 513C (0/00) of the oil is from -10 to -17 0/00 or from -13 to -16 0/00.

[0056] The oils produced according to the above methods in some cases are made using a microalgal host cell. As described above, the microalga can be, without limitation, fall in the classification of Chlorophyta, Trebouxiophyceae , Chlorellales, Chlorellaceae, or Chlorophyceae. It has been found that microalgae of Trebouxiophyceae can be distinguished from vegetable oils based on their sterol profiles. Oil produced by Chlorella protothecoides was found to produce sterols that appeared to be brassicasterol, ergosterol, campesterol, stigmasterol, and β-sitosterol, when detected by GC-MS. However, it is believed that all sterols produced by Chlorella have 024β stereochemistry. Thus, it is believed that the molecules detected as campesterol, stigmasterol, and β-sitosterol, are actually 22,23-dihydrobrassicasterol, proferasterol and clionasterol, respectively. Thus, the oils produced by the microalgae described above can be distinguished from plant oils by the presence of sterols with C24ft stereochemistry and the absence of C24a stereochemistry in the sterols present. For example, the oils produced may contain 22, 23-dihydrobrassicasterol while lacking campesterol; contain clionasterol, while lacking in β-sitosterol, and/or contain poriferasterol while lacking stigmasterol. Alternately, or in addition, the oils may contain significant amounts of Δ7-poriferasterol.

[0057] In one embodiment, the oils provided herein are not vegetable oils. Vegetable oils are oils extracted from plants and plant seeds. Vegetable oils can be distinguished from the non-plant oils provided herein on the basis of their oil content. A variety of methods for analyzing the oil content can be employed to determine the source of the oil or whether adulteration of an oil provided herein with an oil of a different (e.g. plant) origin has occurred. The determination can be made on the basis of one or a combination of the analytical methods. These tests include but are not limited to analysis of one or more of free fatty acids, fatty acid profile, total triacylglycerol content, diacylglycerol content, peroxide values, spectroscopic properties (e.g. UV absorption), sterol profile, sterol degradation products, antioxidants (e.g. tocopherols), pigments (e.g. chlorophyll), dl3C values and sensory analysis (e.g. taste, odor, and mouth feel). Many such tests have been standardized for commercial oils such as the Codex Alimentarius standards for edible fats and oils.

[0058] Sterol profile analysis is a particularly well-known method for determining the biological source of organic matter. Campesterol, b-sitosterol, and stigamsterol are common plant sterols, with b-sitosterol being a principle plant sterol. For example, b-sitosterol was found to be in greatest abundance in an analysis of certain seed oils, approximately 64% in com, 29% in rapeseed, 64% in sunflower, 74% in cottonseed, 26% in soybean, and 79% in olive oil (Gul et al. J. Cell and Molecular Biology 5:71-79, 2006).

[0059] Oil isolated from Prototheca moriformis strain UTEX1435 were separately clarified (CL), refined and bleached (RB), or refined, bleached and deodorized (RBD) and were tested for sterol content according to the procedure described in JAOCS vol. 60, no.8, August 1983. Results of the analysis are shown below (units in mg/lOOg):

[0060] These results show three striking features. First, ergosterol was found to be the most abundant of all the sterols, accounting for about 50% or more of the total sterols. The amount of ergosterol is greater than that of campesterol, β-sitosterol, and stigmasterol combined. Ergosterol is steroid commonly found in fungus and not commonly found in plants, and its presence particularly in significant amounts serves as a useful marker for non-plant oils. Secondly, the oil was found to contain brassicasterol. With the exception of rapeseed oil, brassicasterol is not commonly found in plant based oils. Thirdly, less than 2% β-sitosterol was found to be present. β-sitosterol is a prominent plant sterol not commonly found in microalgae, and its presence particularly in significant amounts serves as a useful marker for oils of plant origin. In summary, Prototheca moriformis strain UTEX1435 has been found to contain both significant amounts of ergosterol and only trace amounts of β-sitosterol as a percentage of total sterol content. Accordingly, the ratio of ergosterol: β-sitosterol or in combination with the presence of brassicasterol can be used to distinguish this oil from plant oils.

[0061] In some embodiments, the oil content of an oil provided herein contains, as a percentage of total sterols, less than 20%, 15%, 10%, 5%, 4%, 3%, 2%, or 1% β-sitosterol. In other embodiments the oil is free from β-sitosterol.

[0062] In some embodiments, the oil is free from one or more of β-sitosterol, campesterol, or stigmasterol. In some embodiments the oil is free from β-sitosterol, campesterol, and stigmasterol. In some embodiments the oil is free from campesterol. In some embodiments the oil is free from stigmasterol.

[0063] In some embodiments, the oil content of an oil provided herein comprises, as a percentage of total sterols, less than 20%, 15%, 10%, 5%, 4%, 3%, 2%, or 1% 24-ethylcholest-5-en-3-ol. In some embodiments, the 24-ethylcholest-5-en-3-ol is clionasterol. In some embodiments, the oil content of an oil provided herein comprises, as a percentage of total sterols, at least 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, or 10% clionasterol.

[0064] In some embodiments, the oil content of an oil provided herein contains, as a percentage of total sterols, less than 20%, 15%, 10%, 5%, 4%, 3%, 2%, or 1% 24-methylcholest-5-en-3-ol. In some embodiments, the 24-methylcholest-5-en-3-ol is 22, 23-dihydrobrassicasterol. In some embodiments, the oil content of an oil provided herein comprises, as a percentage of total sterols, at least 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, or 10% 22,23-dihydrobrassicasterol.

[0065] In some embodiments, the oil content of an oil provided herein contains, as a percentage of total sterols, less than 20%, 15%, 10%, 5%, 4%, 3%, 2%, or 1% 5,22-cholestadien-24-ethyl-3-ol. In some embodiments, the 5, 22-cholestadien-24-ethyl-3-ol is poriferasterol. In some embodiments, the oil content of an oil provided herein comprises, as a percentage of total sterols, at least 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, or 10% poriferasterol.

[0066] In some embodiments, the oil content of an oil provided herein contains ergosterol or brassicasterol or a combination of the two. In some embodiments, the oil content contains, as a percentage of total sterols, at least 5%, 10%, 20%, 25%, 35%, 40%, 45%, 50%, 55%, 60%, or 65% ergosterol. In some embodiments, the oil content contains, as a percentage of total sterols, at least 25% ergosterol. In some embodiments, the oil content contains, as a percentage of total sterols, at least 40% ergosterol. In some embodiments, the oil content contains, as a percentage of total sterols, at least 5%, 10%, 20%, 25%, 35%, 40%, 45%, 50%, 55%, 60%, or 65% of a combination of ergosterol and brassicasterol.

[0067] In some embodiments, the oil content contains, as a percentage of total sterols, at least 1%, 2%, 3%, 4% or 5% brassicasterol. In some embodiments, the oil content contains, as a percentage of total sterols less than 10%, 9%, 8%, 7%, 6%, or 5% brassicasterol.

[0068] In some embodiments the ratio of ergosterol to brassicasterol is at least 5:1, 10:1, 15:1,or 20:1.

[0069] In some embodiments, the oil content contains, as a percentage of total sterols, at least 5%, 10%, 20%, 25%, 35%, 40%, 45%, 50%, 55%, 60%, or 65% ergosterol and less than 20%, 15%, 10%, 5%, 4%, 3%, 2%, or 1% β-sitosterol. In some embodiments, the oil content contains, as a percentage of total sterols, at least 25% ergosterol and less than 5% β-sitosterol. In some embodiments, the oil content further comprises brassicasterol.

[0070] Sterols contain from 27 to 29 carbon atoms (C27 to C29) and are found in all eukaryotes. Animals exclusively make C27 sterols as they lack the ability to further modify the C27 sterols to produce C28 and C29 sterols. Plants however are able to synthesize C28 and C29 sterols, and C28/C29 plant sterols are often referred to as phytosterols. The sterol profile of a given plant is high in C29 sterols, and the primary sterols in plants are typically the C29 sterols b-sitosterol and stigmasterol. In contrast, the sterol profile of non-plant organisms contain greater percentages of C27 and C28 sterols. For example the sterols in fungi and in many microalgae are principally C28 sterols. The sterol profile and particularly the striking predominance of C29 sterols over C28 sterols in plants has been exploited for determining the proportion of plant and marine matter in soil samples (Huang, Wen-Yen, Meinschein W. G., “Sterols as ecological indicators”; Geochimica et Cosmochimia Acta. Vol 43. pp 739-745).

[0071] In some embodiments the primary sterols in the microalgal oils provided herein are sterols other than b-sitosterol and stigmasterol. In some embodiments of the microalgal oils, C29 sterols make up less than 50%, 40%, 30%, 20%, 10%, or 5% by weight of the total sterol content.

[0072] In some embodiments the microalgal oils provided herein contain C28 sterols in excess of C29 sterols. In some embodiments of the microalgal oils, C28 sterols make up greater than 50%, 60%, 70%, 80%, 90%, or 95% by weight of the total sterol content. In some embodiments the C28 sterol is ergosterol. In some embodiments the C28 sterol is brassicasterol.

[0073] In embodiments of the present invention, oleaginous cells expressing one or more of the genes of Table 1 can produce an oil with at least 20,40, 60 or 70% of C8, CIO, C12, C14 or C16 fatty acids. In a specific embodiment, the level of myristatc (C14:0) in the oil is greater than 30%.

[0074] Thus, in embodiments of the invention, there is a process for producing an oil, triglyceride, fatty acid, or derivative of any of these, comprising transforming a cell with any of the nucleic acids discussed herein. In another embodiment, the transformed cell is cultivated to produce an oil and, optionally, the oil is extracted.

Oil extracted in this way can be used to produce food, oleochemicals or other products.

[0075] The oils discussed above alone or in combination are useful in the production of foods, fuels and chemicals (including plastics, foams, films, etc). The oils, triglycerides, fatty acids from the oils may be subjected to C-H activation, hydroamino methylation, methoxy-carbonation, ozonolysis, enzymatic transformations, epoxidation, methylation, dimerization, thiolation, metathesis, hydroalkylation, lactonization, or other chemical processes.

[0076] After extracting the oil, a residual biomass may be left, which may have use as a fuel, as an animal feed, or as an ingredient in paper, plastic, or other product. For example, residual biomass from heterotrophic algae can be used in such products.

Example 1. Discovery of Novel FATB sequences [0077] Sequences of novel plant acyl-ACP thioesterases involved in seed-specific mid-chain (C8-C16) fatty acid biosynthesis in higher plants were isolated. Seed-specific lipid production genes were isolated through direct interrogation of RNA pools accumulating in oilseeds. Based on phylogenetic analysis, novel enzymes can be classified as members of FatB family of acyl-ACP thioesterases.

[0078] Seeds of oleaginous plants were obtained from local grocery stores or requested through USD A ARS National Plant Germplasm System (NPGS) from North Central Regional Plant Introduction Station (NCRIS) or USDA ARS North Central Soil Conservation Research Laboratory (Morris, MI). Dry seeds were homogenized in liquid nitrogen to powder, resuspended in cold extraction buffer containing 6-8M Urea and 3M LiCl and left on ice for a few hours to overnight at 4 °C. The seed homogenate was passed through NucleoSpin Filters (Macherey-Nagel) by centrifugation at 20,000g for 20 minutes in the refrigerated microcentrifuge (4 °C). The resulting RNA pellets were resuspended in the buffer containing 20 mM Tris HC1, pH7.5, 0.5% SDS, 100 mM NaCl, 25 mM EDTA, 2% PVPP) and RNA was subsequently extracted once with Phenol-Chloroform-Isoamyl Alcohol (25:24:1, v/v) and once with chloroform. RNA was finally precipitated with isopropyl alcohol (0.7 Vol.) in the presence of 150 mM of Na Acetate, pH5.2, washed with 80% ethanol by centrifugation, and dried. RNA samples were treated with Turbo DNAse (Lifetech) and purified further using RNeasy kits (Qiagen) following manufacturers’ protocols.

The resulting purified RNA samples were converted to pair-end cDNA libraries and subjected to next-generation sequencing (2xl00bp) using Illumina Hiseq 2000 platform. RNA sequence reads were assembled into corresponding seed transcriptomes using Trinity or Oases packages. Putative thioesterase-containg cDNA contigs were identified by mining transcriptomes for sequences with homology to known thioesterases. These in silico identified putative thioesterase cDNAs have been further verified by direct reverse transcription PCR analysis using seed RNA and primer pairs targeting full-length thioesterase cDNAs. The resulting amplified products were cloned and sequenced de novo to confirm authenticity of identified thioesterase genes.

[0079] To interrogate evolutionary and functional relationship between novel acyl-ACP thioesterases and the members of two existing thioesterase classes (FatA and FatB), we performed a phylogenetic analysis using published full-length (Mayer and Shanklin, 2007) and truncated (THYME database) amino acid thioesterase sequences. Novel proteins appear to group with known acyl-ACP FatB thioesterases involved in biosynthesis of C8-C16 fatty acids. Moreover, novel thioesterases appear to cluster into 3 predominant out-groups suggesting distinct functional similarity and evolutionary relatedness among members of each cluster.

[0080] The amino acid sequences of the FatB genes follow are shown in Table 4.

Table 4: Amino acid sequences of FatB genes:

ChsFATB3j:

MVAAEASSALFSVRTPGTSPKPGKFGNWPTSLSVPFKSKSNHNGGFQV KANASARPKANGSAVSLKSGSLDTQEDTSSSSSPPRTFINQLPDWSMLLSAITT VFVAAEKQWTMLDRKSKRPDMLMDPFGVDRVVQDGAVFRQSFSIRSYEIGA DRT ASIETLMNIF QETSLNHCKSIGLLNDGF GRTPEMCKRDLIWVVTKMHIE V NRYPTWGDTIEVNTWVSESGKTGMGRDWLISDFHTGDILIRATSVCAMMNQ KTRRF SKFP YE VRQEL APHF VD SAP VIED Y QKLHKLD VKT GD SICN GLTPRW NDLDVNQHVNNVKYIGWILESVPTEVFETQELCGLTLEYRQECGRDSVLESV T AMDP SKEGDRSL Y QHLLRLEDGTDIAKGRTKWRPKN AGKT SN GN SIS

Example 2. Cloning and fatty acid analysis of cells transformed with novel FATB genes [0081] In the example below, we detail the effect of expressing plant oilseed transcriptome-derived, heterologous thioesterases in the UTEX1435 (web.biosci.utexas.edu/utex/) strain, Strain A.

[0082] As in Example 1, RNA was extracted from dried plant seeds and submitted for paired-end sequencing using the Illumina Hiseq 2000 platform. RNA sequence reads were assembled into corresponding seed transcriptomes using Trinity or Oases packages and putative thioesterase-containing cDNA contigs were identified by mining transcriptomes for sequences with homology to known thioesterases.

These in silico identified putative thioesterase cDNAs were verified by direct reverse transcription PCR analysis using seed RNA and primer pairs targeting full-length thioesterase cDNAs. The resulting amplified products were cloned and sequenced de novo to confirm authenticity of identified thioesterase genes and to identify sequence variants arising from expression of different gene alleles or diversity of sequences within a population of seeds. The resulting amino acid sequences were subjected to phylogenetic analysis using published full-length (Mayer and Shanklin, 2007) and truncated (THYME database) FatB sequences. The thioesterases that clustered with acyl-ACP FatB thioesterases, which are involved in biosynthesis of C8-C16 fatty acids, were pursued.

Construction of Transforming Vectors Expressing Acyl-ACP FatB Thioesterases [0083] 27 putative acyl-ACP FatB thioesterases from the species Cinnamomum camphora, Cuphea hyssopifolia, Cuphea PSR23, Cuphea wrightii, Cuphea heterophylla, and Cuphea viscosissima were synthesized in a codon-optimized form to reflect Prototheca moriformis (UTEX 1435) codon usage. Of the 27 genes synthesized, 24 were identified by our transcriptome sequencing efforts and the 3 genes from Cuphea viscosissima, were from published sequences in GenBank.

[0084] Transgenic strains were generated via transformation of the base strain Strain A (Prototheca moriformis, derived from UTEX 1435 by classical mutation and screening for high oil production) with a construct encoding 1 of the 27 FatB thioesterases. The construct pSZ2760 encoding Cinnamomum camphora (Cc) FATB lb is shown as an example, but identical methods were used to generate each of the remaining 26 constructs encoding the different respective thioesterases. Construct pSZ2760 can be written as 6S::CrTUB2:ScSUC2:CvNR::PmAMT3:CcFATBlb:CvNR::6S. The sequence of the transforming DNA is provided in Table 5 (pSZ2760). The relevant restriction sites in the construct from 5’-3’, BspQl, Kpnl, AscI, Mfel, EcoRI, Spel, Xhol, SacI, BspQl, respectively, are indicated in lowercase, bold, and underlined. BspQl sites delimit the 5 ’ and 3 ’ ends of the transforming DNA. Bold, lowercase sequences at the 5 ’ and 3 ’ end of the construct represent genomic DNA from UTEX 1435 that target integration to the 6S locus via homologous recombination. Proceeding in the 5 ’ to 3 ’ direction, the selection cassette has the C. reinhardtii β-tubulin promoter driving expression of the S. cerevisiae gene SUC2 (conferring the ability to grow on sucrose) and the Chlorella vulgaris Nitrate Reductase (NR) gene 3 ’ UTR. The promoter is indicated by lowercase, boxed text. The initiator ATG and terminator TGA for ScSUC2 are indicated by bold, uppercase italics, while the coding region is indicated with lowercase italics. The 3’ UTR is indicated by lowercase underlined text. The spacer region between the two cassettes is indicated by upper case text. The second cassette containing the codon optimized CcFATBlb gene (Table 5; pSZ2760) from Cinnamomum camphora is driven by the Prototheca moriformis endogenous AMT3 promoter, and has the Chlorella vulgaris Nitrate Reductase (NR) gene 3’ UTR. In this cassette, the AMT3 promoter is indicated by lowercase, boxed text. The initiator ATG and terminator TGA for the CcFATBlb gene are indicated in bold, uppercase italics, while the coding region is indicated by lowercase italics and the spacer region is indicated by upper case text. The 3’ UTR is indicated by lowercase underlined text. The final construct was sequenced to ensure correct reading frame and targeting sequences.

Table 5: pSZ2760 Transforming construct gctcttcgccgccgccactcctgctcgagcgcgcccgcgcgtgcgccgccagcgccttggccttttcgccgcgctcgtgc gcgtcgctgatgtccatcaccaggtccatgaggtctgccttgcgccggctgagccactgcttcgtccgggcggccaagag gagcatgagggaggactcctggtccagggtcctgacgtggtcgcggctctgggagcgggccagcatcatctggctctgc cgcaccgaggccgcctccaactggtcctccagcagccgcagtcgccgccgaccctggcagaggaagacaggtgaggg gggtatgaattgtacagaacaaccacgagccttgtctaggcagaatccctaccagtcatggctttacctggatgacggcctg cgaacagctgtccagcgaccctcgctgccgccgcttctcccgcacgcttctttccagcaccgtgatggcgcgagccagcg ccgcacgctggcgctgcgcttcgccgatctgaggacagtcggggaactctgatcagtctaaacccccttgcgcgttagtgtt gccatcctttgcagaccggtgagagccgacttgttgtgcgccaccccccacaccacctcctcccagaccaattctgtcacct ttttggcgaaggcatcggcctcggcctgcagagaggacagcagtgcccagccgctgggggttggcggatgcacgctca ggtacdctttcttgcgctatgacacttccagcaaaaggtagggcgggctgcgagacggcttcccggcgctgcatgcaaca ccgatgatgcttcgaccccccgaagctccttcggggctgcatgggcgctccgatgccgctccagggcgagcgctgtttaa atagccaggcccccgattgcaaagacattatagcgagctaccaaagccatattcaaacacctagatcactaccacttctaca caggccactcgagcttgtgatcgcactccgctaagggggcgcctcttcctcttcgtttcagtcacaacccgcaaaqggcgc gccATGctgctgcaggccttcctgttcctgctggccggcttcgccgccaagatcagcgcctccatgacgaacgagac gtccgaccgccccctggtgcacttcacccccaacaagggctggatgaacgaccccaacggcctgtggtacgacgag aaggacgccaagtggcacctgtacttccagtacaacccgaacgacaccgtctgggggacgcccttgttctggggcca cgccacgtccgacgacctgaccaactgggaggaccagcccatcgccatcgccccgaagcgcaacgactccggcgc cttctccggctccatggtggtggactacaacaacacctccggcttcttcaacgacaccatcgacccgcgccagcgctgc gtggccatctggacctacaacaccccggagtccgaggagcagtacatctcctacagcctggacggcggctacaccttc accgagtaccagaagaaccccgtgctggccgccaactccacccagttccgcgacccgaaggtcttctggtacgagcc ctcccagaagtggatcatgaccgcggccaagtcccaggactacaagatcgagatctactcctccgacgacctgaagt cctggaagctggagtccgcgttcgccaacgagggcttcctcggctaccagtacgagtgccccggcctgatcgaggtcc ccaccgagcaggaccccagcaagtcctactgggtgatgttcatctccatcaaccccggcgccccggccggcggctcct tcaaccagtacttcgtcggcagcttcaacggcacccacttcgaggccttcgacaaccagtcccgcgtggtggacttcgg caaggactactacgccctgcagaccttcttcaacaccgacccgacctacgggagcgccctgggcatcgcgtgggcctc caactgggagtactccgccttcgtgcccaccaacccctggcgctcctccatgtccctcgtgcgcaagttctccctcaaca ccgagtaccaggccaacccggagacggagctgatcaacctgaaggccgagccgatcctgaacatcagcaacgccg gcccctggagccggttcgccaccaacaccacgttgacgaaggccaacagctacaacgtcgacctgtccaacagcac cggcaccctggagttcgagctggtgtacgccgtcaacaccacccagacgatctccaagtccgtgttcgcggacctctcc ctctggttcaagggcctggaggaccccgaggagtacctccgcatgggcttcgaggtgtccgcgtcctccttcttcctgga ccgcgggaacagcaaggtgaagttcgtgaaggagaacccctacttcaccaaccgcatgagcgtgaacaaccagcc cttcaagagcgagaacgacctgtcctactacaaggtgtacggcttgctggaccagaacatcctggagctgtacttcaac gacggcgacgtcgtgtccaccaacacctacttcatgaccaccgggaacgccctgggctccgtgaacatgacgacggg ggtggacaacctgttctacatcgacaagttccaggtgcgcgaggtcaagTGAcaattzzcazcazcazctczzataz tatcgacacactctggacgctggtcgtgtgatggactgttgccgccacacttgctgccttgacctgtgaatatccctgccgctt ttatcaaacagcctcagtgtgtttgatcttgtgtgtacgcgcttttgcgagttgctagctgcttgtgctatttgcgaataccacccc cagcatccccttccctcgtttcatatcgcttgcatcccaaccgcaacttatctacgctgtcctgctatccctcagcgctgctcct gctcctgctcactgcccctcgcacagccttggtttgggctccgcctgtattctcctggtactgcaacctgtaaaccagcactg caatgctgatgcacgggaagtagtgggatgggaacacaaatggaAAGCT GT AT AGGG AT AAgaattcIggl ccgacaggacgcgcgtcaaaggtgctggtcgtgtatgccctggccggcaggtcgttgctgctgctggttagtgattccgca accctgattttggcgtcttattttggcgtggcaaacgctggcgcccgcgagccgggccggcggcgatgcggtgccccacg gctgccggaatccaagggaggcaagagcgcccgggtcagttgaagggctttacgcgcaaggtacagccgctcctgcaa ggctgcgtggtggaattggacgtgcaggtcctgctgaagttcctccaccgcctcaccagcggacaaagcaccggtgtatc aggtccgtgtcatccactctaaagaactcgactacgacctactgatggccctagattcttcatcaaaaacgcctgagacactt gcccaggattgaaactccctgaagggaccaccaggggccctgagttgttccttccccccgtggcgagctgccagccagg ctgtacctgtgatcgaggctggcgggaaaataggcttcgtgtgctcaggtcatgggaggtgcaggacagctcatgaaacg ccaacaatcgcacaattcatgtcaagctaatcagctatttcctcttcacgagctgtaattgtcccaaaattctggtctaccgggg gtgatccttcgtgtacgggcccttccctcaaccctaggtatgcgcgcatgcggtcgccgcgcaactcgcgcgagggccga gggtttgggacgggccgtcccgaaatgcagttgcacccggatgcgtggcaccttttttgcgataatttatgcaatggactgct ctgcaaaattctggctctgtcgccaaccctaggatcagcggcgtaggatttcgtaatcattcgtcctgatggggagctaccg actaccctaatatcagcccgactgcctgacgccagcgtccacttttgtgcacacattccattcgtgcccaagacatttcattgt ggtgcgaagcgtccccagttacgctcacctgtttcccgacctccttactgttctgtcgacagagcgggcccacaggccggt cgcagcmctaetATGgccaccacctccctggcctccgccttctgctccatgaaggccgtgatgctggcccgcgacg gccgcggcctgaagccccgctcctccgacctgcagctgcgcgccggcaacgcccagacctccctgaagatgatcaac ggcaccaagttctcctacaccgagtccctgaagaagctgcccgactggtccatgctgttcgccgtgatcaccaccatctt ctccgccgccgagaagcagtggaccaacctggagtggaagcccaagcccaaccccccccagctgctggacgacca cttcggcccccacggcctggtgttccgccgcaccttcgccatccgctcctacgaggtgggccccgaccgctccacctcc atcgtggccgtgatgaaccacctgcaggaggccgccctgaaccacgccaagtccgtgggcatcctgggcgacggctt cggcaccaccctggagatgtccaagcgcgacctgatctgggtggtgaagcgcacccacgtggccgtggagcgctacc ccgcctggggcgacaccgtggaggtggagtgctgggtgggcgcctccggcaacaacggccgccgccacgacttcct ggtgcgcgactgcaagaccggcgagatcctgacccgctgcacctccctgtccgtgatgatgaacacccgcacccgcc gcctgtccaagatccccgaggaggtgcgcggcgagatcggccccgccttcatcgacaacgtggccgtgaaggacga ggagatcaagaagccccagaagctgaacgactccaccgccgactacatccagggcggcctgaccccccgctggaa cgacctggacatcaaccagcacgtgaacaacatcaagtacgtggactggatcctggagaccgtgcccgactccatctt cgagtcccaccacatctcctccttcaccatcgagtaccgccgcgagtgcacccgcgactccgtgctgcagtccctgacc accgtgtccggcggctcctccgaggccggcctggtgtgcgagcacctgctgcagctggagggcggctccgaggtgct gcgcgccaagaccgagtggcgccccaagctgtccttccgcggcatctccgtgatccccgccgagtcctccgtgatgga ctacaaggaccacgacggcgactacaaggaccacgacatcgactacaaggacgacgacgacaagTGAclcga ggcagcagcagctcggatagtatcgacacactctggacgctggtcgtgtgatggactgttgccgccacacttgctgccttg acctgtgaatatccctgccgcttttatcaaacagcctcagtgtgtttgatcttgtgtgtacgcgcttttgcgagttgctagctgctt gtgctatttgcgaataccacccccagcatccccttccctcgtttcatatcgcttgcatcccaaccgcaacttatctacgctgtcc tgctatccctcagcgctgctcctgctcctgctcactgcccctcgcacagccttggtttgggctccgcctgtattctcctggtact

gcaacctgtaaaccagcactgcaatgctgatgcacgggaagtagtgggatgggaacacaaatggaAAGCTGTAT AGGGATAACAGGGTAATgagctcttgttttccagaaggagttgctccttgagcctttcattctcagcctcgata acctccaaagccgctctaattgtggagggggttcgaatttaaaagcttggaatgttggttcgtgcgtctggaacaagcccag acttgttgctcactgggaaaaggaccatcagctccaaaaaacttgccgctcaaaccgcgtacctctgctttcgcgcaatctgc cctgttgaaatcgccaccacattcatattgtgacgcttgagcagtctgtaattgcctcagaatgtggaatcatctgccccctgtg cgagcccatgccaggcatgtcgcgggcgaggacacccgccactcgtacagcagaccattatgctacctcacaatagttca taacagtgaccatatttctcgaagctccccaacgagcacctccatgctctgagtggccaccccccggccctggtgcttgcg gagggcaggtcaaccggcatggggctaccgaaatccccgaccggatcccaccacccccgcgatgggaagaatctctcc ccgggatgtgggcccaccaccagcacaacctgctggcccaggcgagcgtcaaaccataccacacaaatatccttggcat cggccctgaattccttctgccgctctgctacccggtgcttctgtccgaagcaggggttgctagggatcgctccgagtccgca aacccttgtcgcgtggcggggcttgttcgagcttgaagagc [0085] Constmcts encoding the identified heterologous FatB genes, such as

CcFATBlb from pSZ2760 in Table 6, were transformed into Strain A, and selected for the ability to grow on sucrose. Transformations, cell culture, lipid production and fatty acid analysis were all carried out as previously described. After cultivating on sucrose under low nitrogen conditions to accumulate oil, fatty acid profiles were determined by FAME-GC. The top performer from each transformation, as judged by the ability to produce the highest level of midchain fatty acids, is shown in Table 4.

Table 6: Alteration of Fatty Acid Profiles in S3150 upon Expression of Heterologous FatB Thioesterases

[0086] Many of the acyl-ACP FatB thioesterases were found to exhibit midchain activity when expressed in Prototheca moriformis. For example, expression of CcFATBlb causes an increase in myristate levels from 2% of total fatty acids in the parent, Strain A, to ~ 15% in the D1670-13 primary transformant. Other examples include CcFATB4, which exhibits an increase in laurate levels from 0% in Strain A to ~33%, and ChsFATB3, which exhibits an increase in myristate levels to ~34%. Although some of the acyl-ACP thioesterases did not exhibit dramatic effects on midchain levels in the current incarnation, efforts will likely develop to optimize some of these constructs.

[0087] Sequences of the Heterologous Acyl-ACP Thioesterases Identified and Transformed into P. moriformis (UTEX 1435) [0088] A complete listing of relevant sequences for the transforming constructs, such as the deduced amino acid sequence of the encoded acyl-ACP thioesterase, the native CDS coding sequence, the Prototheca moriformis codon-optimized coding sequence, and the nature of the sequence variants examined, is provided as SEQ ID NOS: 1-78.

Example 3. Discovery and Cloning of Additional FATB genes

Additional FATB genes were obtained from seeds as described above. The species and number of FatB genes identified were:

[0089] The thioesterases that clustered with acyl-ACP FatB thioesterases, which are involved in biosynthesis of C8-C16 fatty acids, were pursued. The native, putative plastid-targeting transit peptide sequence is indicated by underlining.

[0090] Construction of Transforming Vectors Expressing Acyl-ACP FatB Thioesterases. The nine putative Acyl-ACP FatB Thioesterases from the species Cuphea calcarata, Cuphea painter, Cuphea hookeriana, Cuphea avigera var. pulcherrima, Cuphea paucipetala, Cuphea procumbens, and Cuphea ignea were synthesized in a codon-optimized form to reflect UTEX 1435 codon usage. In contrast to the previous example, the new Acyl-ACP FatB thioesterases were synthesized with a modified transit peptide from Chlorella protothecoides (Cp) in place of the native transit peptide. The modified transit peptide derived from the CpSADl gene, “CpSADltp trimmed”, was synthesized as an in-frame, N-terminal fusion to the FatB acyl-ACP thioesterases in place of the native transit peptide; the resulting sequences are listed below. The novel FatB genes were cloned into Prototheca moriformis as described above. Constructs encoding heterologous FatB genes were transformed into strain S6165 (a descendant of S3150/Strain A) and selected for the ability to grow on sucrose. Transformations, cell culture, lipid production and fatty acid analysis were all carried out as previously described. The results for the nine novel FatB acyl-ACP thioesterases are displayed in the table immediately below.

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[0092] CaFATBl, which exhibits high C8:0 and 00:0 levels, is of particular interest. CaFATBl arose from two separate contigs that were assembled from the Cupha avigera var. pulcherrima transcriptome, S17_Cavig_trinity_7406 and S17_Cavig_trinity_7407. Although the two partial contigs exhibit only 17 nucleotides of overlap, we were able to assemble a putative full length transcript encoding CaFATBl from the two contigs and then subsequently confirm the existence of the full-length transcript by direct reverse transcription PCR analysis using seed RNA and primer pairs targeting the full-length CaFATBl thioesterase cDNA. Tjellstrom et al. (2013) discloses the expression of a newly identified fatty acyl-ACP thioesterase from Cuphea pulcherrima that they named “CpuFATB3” (Genbank accession number KC675178). The coding sequence of CpuFATB3 is 100% identical to the CaFATBl gene we identified and contains one nucleotide difference in the RNA sequence outside the predicted coding region. Tjellstrom et al. (2013) showed that CpuFATB3 produces an average of 4.8% C8:0 when expressed in Arabidopsis, and further requires deletion of two acyl-ACP synthetases, AAE15/16, to produce an average of 9.2% C8:0 with a maximum level of-12% C8.0. The CaFATBl gene we identified was codon-optimized for expression in UTEX1435 and generated as a CpSADltp-trimmed transit peptide fusion before introduction into S6165. The CpSADltp_trimmed:CaFATBl gene produces an average C8:0 level of 14% and a maximum level of 22% C8:0 without requiring the deletion of endogenous acyl-ACP synthetases.

[0093] Table 7. Amino Acid Sequences of Additional Novel FatB Acyl-ACP Thioesterases. In the appended sequence listings, the native, putative plastid-targeting transit peptide sequence is underlined:

Example 4. FATB consensus sequences: Discovery, cloning and fatty acid profiles

[0094] In the course of testing several new putative midchain FatB thioesterases in UTEX1435, S3150 (Strain A above), we identified several thioesterases with increased C10:0 and C16:0 activity above the background midchain levels found in the strain. We reasoned that a consensus sequence could be obtained for an idealized C10:0 thioesterase and C16:0 thioesterase from aligning the bestperforming 00:0 and 06:0 thioesterases. A consensus 00:0 specific thioesterase sequence was generated using the C. palustris FatBl (CpFATBl), C. PSR23 FatB3 (CuPSR23FATB3), C. viscosissima FatBl (CvisFATBl), C. glossostoma FatBl (CgFATBl), and C. carthagenensis FatB2 (CcrFATB2) sequences as inputs resulting in a 00:0 specific consensus sequence termed JcFATBl/SzFATBl. A consensus 06:0 specific thioesterase sequence was generated using the C. heterophylla FatB3a (ChtFATB3a), C. carthagenensis FatBl (CcrFATBl), C. viscosissima FatB2 (CvisFATB2), C. hookeriana FatBl (ChFATBl; AAC48990), C. hyssopifolia FatB2 (ChsFATB2), C. calophylla FatB2 (CcalFATB2; ABB71581), C. hookeriana FatBl-1 (ChFATBl-1; AAC72882), C. lanceolata FatBl (C1FATB1; CAC19933), and C. wrightii FatB4a (CwFATB4a) sequences as inputs resulting in a 06:0 specific consensus sequence termed JcFATB2/SzFATB2. The resulting consensus sequences were synthesized, cloned into a vector identical to that used to test other FatB thioesterases, and introduced into S3150 as described above. The consensus amino acid sequences are given as SEQ ID NOs. 106 and 107; the nucleic acid sequences were based on these amino acid sequences using codon optimization for Prototheca moriformis. The transformants were selected, cultivated and the oil was extracted and analyzed by FAME-GC-FID. The fatty acid profiles obtained are given in the table below.

Example 5: Clade analysis

Various novel FATB thioesterases were clustered according to a neighbor joining algorithm. These were found to form twelve clades as listed in Table la. Putative function was assigned based on expression in Prototheca as described above.

[0095] The described embodiments of the invention are intended to be merely exemplary and numerous variations and modifications will be apparent to those skilled in the art. All such variations and modifications are intended to be within the scope of the present invention.

Sequence Listing SEQ ID NO 1:

Cinnamomum camphora (Cc) FATBlb variant M25L, M322R, AT367-D368 amino acid sequence

MATTSLASAFCSMKAVMLARDGRGLKPRSSDLQLRAGNAQTSLKMINGTKFSYTESLKKLPD

WSMLFAVITTIFSAAEKQWTNLEWKPKPNPPQLLDDHFGPHGLVFRRTFAIRSYEVGPDRSTSI

VAVMNHLQEAALNHAKS VGILGDGF GTTLEM SKRDLIWVVKRTHVAVERYPAW GDT VEVE

CWVGASGNNGRRHDFLVRDCKTGEILTRCTSLSVMMNTRTRRLSKIPEEVRGEIGPAFIDNVA

VKDEEIKKPQKLNDSTADYIQGGLTPRWNDLDINQHVNNIKYVDWILETVPDSIFESHHISSFTI

EYRRECTRDSVLQSLTTVSGGSSEAGLVCEHLLQLEGGSEVLRAKTEWRPKLSFRGISVIPAES sv* SEQ ID NO 2:

Cinnamomum camphora (Cc) FATBlb variant M25L, M322R, AT367-D368 coding DNA sequence

TTAGCTTCTGCTTTCTGCTCGATGAAAGCTGTAATGTTGGCTCGTGATGGCAGGGGCTTGA

AACCCAGGAGCAGTGATTTGCAGCTGAGGGCGGGAAATGCACAAACCTCTTTGAAGATGA

TCAATGGGACCAAGTTCAGTTACACAGAGAGCTTGAAAAAGTTGCCTGACTGGAGCATGC

TCTTTGCAGTGATCACGACCATCTTTTCGGCTGCTGAGAAGCAGTGGACCAATCTAGAGTG

GAAGCCGAAGCCGAATCCACCCCAGTTGCTTGATGACCATTTTGGGCCGCATGGGTTAGTT

TTCAGGCGCACCTTTGCCATCAGATCGTATGAGGTGGGACCTGACCGCTCCACATCTATAG

TGGCTGTTATGAATCACTTGCAGGAGGCTGCACTTAATCATGCGAAGAGTGTGGGAATTCT

AGGAGATGGATTCGGTACGACGCTAGAGATGAGTAAGAGAGATCTGATATGGGTTGTGAA

ACGCACGCATGTTGCTGTGGAACGGTACCCTGCTTGGGGTGATACTGTTGAAGTAGAGTG

CTGGGTTGGTGCATCGGGAAATAATGGCAGGCGCCATGATTTCCTTGTCCGGGACTGCAA

AACAGGCGAAATTCTTACAAGATGTACCAGTCTTTCGGTGATGATGAATACAAGGACAAG

GAGGTTGTCCAAAATCCCTGAAGAAGTTAGAGGGGAGATAGGGCCTGCATTCATTGATAA

TGTGGCTGTCAAGGACGAGGAAATTAAGAAACCACAGAAGCTCAATGACAGCACTGCAG

ATTACATCCAAGGAGGATTGACTCCTCGATGGAATGATTTGGATATCAATCAGCACGTTA

ACAACATCAAATACGTTGACTGGATTCTTGAGACTGTCCCAGACTCAATCTTTGAGAGTCA

TCATATTTCCAGCTTCACTATTGAATACAGGAGAGAGTGCACGAGGGATAGCGTGCTGCA

GTCCCTGACCACTGTCTCCGGTGGCTCGTCGGAAGCTGGGTTAGTGTGCGAGCACTTGCTC

CAGCTTGAAGGTGGGTCTGAGGTATTGAGGGCAAAAACAGAGTGGAGGCCTAAGCTTAGT

TTCAGAGGGATTAGTGTGATACCCGCAGAATCGAGTGTCTAA SEQ ID NO 3:

Cinnamomum camphora (Cc) FATBlb variant M25L, M322R, ΔΤ367-0368 coding DNA sequence codon optimized for Prototheca moriformis

TTAGCTTCTGCTTTCTGCTCGATGAAAGCTGTAATGTTGGCTCGTGATGGCAGGGGCTTGA

AACCCAGGAGCAGTGATTTGCAGCTGAGGGCGGGAAATGCACAAACCTCTTTGAAGATGA

TCAATGGGACCAAGTTCAGTTACACAGAGAGCTTGAAAAAGTTGCCTGACTGGAGCATGC

TCTTTGCAGTGATCACGACCATCTTTTCGGCTGCTGAGAAGCAGTGGACCAATCTAGAGTG

GAAGCCGAAGCCGAATCCACCCCAGTTGCTTGATGACCATTTTGGGCCGCATGGGTTAGTT

TTCAGGCGCACCTTTGCCATCAGATCGTATGAGGTGGGACCTGACCGCTCCACATCTATAG

TGGCTGTTATGAATCACTTGCAGGAGGCTGCACTTAATCATGCGAAGAGTGTGGGAATTCT

AGGAGATGGATTCGGTACGACGCTAGAGATGAGTAAGAGAGATCTGATATGGGTTGTGAA

ACGCACGCATGTTGCTGTGGAACGGTACCCTGCTTGGGGTGATACTGTTGAAGTAGAGTG

CTGGGTTGGTGCATCGGGAAATAATGGCAGGCGCCATGATTTCCTTGTCCGGGACTGCAA

AACAGGCGAAATTCTTACAAGATGTACCAGTCTTTCGGTGATGATGAATACAAGGACAAG

GAGGTTGTCCAAAATCCCTGAAGAAGTTAGAGGGGAGATAGGGCCTGCATTCATTGATAA

TGTGGCTGTCAAGGACGAGGAAATTAAGAAACCACAGAAGCTCAATGACAGCACTGCAG

ATTACATCCAAGGAGGATTGACTCCTCGATGGAATGATTTGGATATCAATCAGCACGTTA

ACAACATCAAATACGTTGACTGGATTCTTGAGACTGTCCCAGACTCAATCTTTGAGAGTCA

TCATATTTCCAGCTTCACTATTGAATACAGGAGAGAGTGCACGAGGGATAGCGTGCTGCA

GTCCCTGACCACTGTCTCCGGTGGCTCGTCGGAAGCTGGGTTAGTGTGCGAGCACTTGCTC

CAGCTTGAAGGTGGGTCTGAGGTATTGAGGGCAAAAACAGAGTGGAGGCCTAAGCTTAGT

TTCAGAGGGATTAGTGTGATACCCGCAGAATCGAGTGTCTAA SEQ ID N0:4

Cinnamomum camphor a (Cc) FATB4 amino acid sequence

MVTTSLASAYFSMKAVMLAPDGRGIKPRSSGLQVRAGNERNSCKVINGTKVKDTEGLKGCST LQGQSMLDDHF GLHGLVFRRTF AIRC YE VGPDRST SIMAVMNHLQEAARNHAE SLGLLGDGF GETLEMSKRDLIWWRRTHVAVERYPAWGDTVEVEAWVGASGNTGMRRDFLVRDCKTGHI LTRCTSVSVMMNMRTRRLSKIPQEVRAEIDPLFIEKVAVKEGEIKKLQKLNDSTADYIQGGWT PRWNDLDVNQHVNNIIYVGWIFKSVPDSISENHHLSSITLEYRRECTRGNKLQSLTTVCGGSSE AGIICEHLLQLEDGSEVLRARTEWRPKHTDSFQGISERFPQQEPHK SEQ ID NO: 5

Cinnamomum camphor a (Cc) FATB4 coding DNA sequence

ATGGTCACCACCTCTTTAGCTTCCGCTTACTTCTCGATGAAAGCTGTAATGTTGGCTCCTGA

CGGCAGGGGCATAAAGCCCAGGAGCAGTGGTTTGCAGGTGAGGGCGGGAAATGAACGAA

ACTCTTGCAAGGTGATCAATGGGACCAAGGTCAAAGACACGGAGGGCTTGAAAGGGTGC

AGC ACGTTGCAAGGCCAGAGC ATGCTT GAT GACCATTTTGGT CT GC ATGGGCT AGTTTTCA

GGCGCACCTTTGCAATCAGATGCTATGAGGTTGGACCTGACCGCTCCACATCCATAATGGC

TGTTATGAATCACTTGCAGGAAGCTGCACGTAATCATGCGGAGAGTCTGGGACTTCTAGG

AGATGGATTCGGTGAGACACTGGAGATGAGTAAGAGAGATCTGATATGGGTTGTGAGACG

CACGCATGTTGCTGTGGAACGGTACCCTGCTTGGGGCGATACTGTTGAAGTCGAGGCCTG

GGTGGGTGCATCAGGTAACACTGGCATGCGCCGCGATTTCCTTGTCCGCGACTGCAAAAC

TGGCCACATTCTTACAAGATGTACCAGTGTTTCAGTGATGATGAATATGAGGACAAGGAG

ATTGTCCAAAATTCCCCAAGAAGTTAGAGCGGAGATTGACCCTCTTTTCATTGAAAAGGTT

GCTGTCAAGGAAGGGGAAATTAAAAAATTACAGAAGTTGAATGATAGCACTGCAGATTAC

ATTC AAGGGGGTTGGACTCCTCGAT GGAAT GATTT GGAT GTC AATCAGC ACGT GAAC AAT

ATCATATACGTTGGCTGGATTTTTAAGAGCGTCCCAGACTCTATCTCTGAGAATCATCATC

TTTCTAGCATCACTCTCGAATACAGGAGAGAGTGCACAAGGGGCAACAAGCTGCAGTCCC

TGACCACTGTTTGTGGTGGCTCGTCGGAAGCTGGGATCATATGTGAGCACCTACTCCAGCT

TGAGGATGGGTCTGAGGTTTTGAGGGCAAGAACAGAGTGGAGGCCCAAGCACACCGATA

GTTTCC AAGGC ATTAGT GAGAGATTCCCGCAGCAAGAACCGCAT AAGT AA SEQ ID NO: 6

Cinnamomum camphora (Cc) FATB4 coding DNA sequence codon optimized for Prototheca moriformis

ATGGTGACCACCTCCCTGGCCTCCGCCTACTTCTCCATGAAGGCCGTGATGCTGGCCCCCG

ACGGCCGCGGCATCAAGCCCCGCTCCTCCGGCCTGCAGGTGCGCGCCGGCAACGAGCGCA

ACTCCTGCAAGGT GATCAACGGCACCAAGGTGAAGGAC ACCGAGGGCCT GAAGGGCT GC

TCCACCCTGCAGGGCCAGTCCATGCTGGACGACCACTTCGGCCTGCACGGCCTGGTGTTCC

GCCGCACCTTCGCCATCCGCTGCTACGAGGTGGGCCCCGACCGCTCCACCTCCATCATGGC

CGTGATGAACCACCTGCAGGAGGCCGCCCGCAACCACGCCGAGTCCCTGGGCCTGCTGGG

CGACGGCTTCGGCGAGACCCTGGAGATGTCCAAGCGCGACCTGATCTGGGTGGTGCGCCG

CACCCACGTGGCCGTGGAGCGCTACCCCGCCTGGGGCGACACCGTGGAGGTGGAGGCCTG

GGTGGGCGCCTCCGGCAACACCGGCATGCGCCGCGACTTCCTGGTGCGCGACTGCAAGAC

CGGCCACATCCTGACCCGCTGCACCTCCGTGTCCGTGATGATGAACATGCGCACCCGCCGC

CTGTCCAAGATCCCCCAGGAGGTGCGCGCCGAGATCGACCCCCTGTTCATCGAGAAGGTG

GCCGTGAAGGAGGGCGAGATCAAGAAGCTGCAGAAGCTGAACGACTCCACCGCCGACTA

CATCCAGGGCGGCTGGACCCCCCGCTGGAACGACCTGGACGTGAACCAGCACGTGAACAA

CATCATCTACGTGGGCTGGATCTTCAAGTCCGTGCCCGACTCCATCTCCGAGAACCACCAC

CTGTCCTCCATCACCCTGGAGTACCGCCGCGAGTGCACCCGCGGCAACAAGCTGCAGTCC

CTGACCACCGTGTGCGGCGGCTCCTCCGAGGCCGGCATCATCTGCGAGCACCTGCTGCAG

CTGGAGGACGGCTCCGAGGTGCTGCGCGCCCGCACCGAGTGGCGCCCCAAGCACACCGAC

TCCTTCCAGGGCATCTCCGAGCGCTTCCCCCAGCAGGAGCCCCACAAGTGA SEQ ID NO: 7

Cinnamomum camphora (Cc) FATB3 amino acid sequence

MVAT AAAS AFFPVGAPAT S S ATS AKASMMPDNLDARGIKPKPAS S SGLQVKANAHASPKING

SKVSTDTLKGEDTLTSSPAPRTFINQLPDWSMFLAAITTIFLAAEKQWTNLDWKPRRPDMLAD

PFGIGRFMQDGLIFRQHFAIRSYEIGADRTASIETLMNHLQETALNHVRSAGLLGDGFGATPEM

SRRDLIWVVTRMQVLVDRYPAWGDIVEVETWVGASGKNGMRRDWLVRDSQTGEILTRATSV

WVMMNKRTRRLSKLPEEVRGEIGPYFIEDVAIIEEDNRKLQKLNENTADNVRRGLTPRWSDLD

VNQHVNNVKYIGWILESAPGSILESHELSCMTLEYRRECGKDSVLQSMTAVSGGGSAAGGSPE

S S VECDHLLQLE SGPEWRGRTE WRPKS ANN SRSILEMPAE SL SEQ ID NO: 8

Cinnamomum camphora (Cc) FATB3 coding DNA sequence

ATGGTTGCCACCGCTGCTGCTTCTGCTTTCTTCCCGGTCGGTGCTCCGGCTACGTCATCTGC

AACTTCAGCCAAAGCGTCGATGATGCCTGATAATTTGGATGCCAGAGGCATCAAACCGAA

GCCGGCTTCGTCCAGCGGCTTGCAGGTTAAGGCAAATGCCCATGCCTCTCCCAAGATTAAT

GGTTCCAAGGTGAGCACGGATACCTTGAAGGGGGAAGACACCTTAACTTCCTCGCCCGCC

CCACGGACCTTTATCAACCAATTGCCTGACTGGAGCATGTTCCTTGCTGCCATCACAACTA

TTTTCTTGGCTGCCGAGAAGCAGTGGACGAATCTCGACTGGAAGCCCAGAAGACCCGACA

TGCTTGCTGACCCGTTTGGCATCGGGAGGTTTATGCAGGATGGGCTGATTTTCAGGCAGCA

CTTTGCAATCAGATCTTATGAGATTGGGGCTGATAGAACGGCGTCTATAGAGACTTTAATG

AATCACTTGCAGGAGACTGCACTTAATCATGTGAGGAGTGCTGGACTCCTAGGTGATGGA

TTTGGTGCGACACCTGAGATGAGTAGAAGAGATCTGATATGGGTTGTAACACGTATGCAG

GTTCTTGTGGACCGCTACCCTGCTTGGGGTGATATTGTTGAAGTAGAGACCTGGGTTGGTG

CATCTGGAAAAAATGGTATGCGCCGTGATTGGCTTGTTCGGGACAGCCAAACTGGTGAAA

TTCTCACACGAGCTACCAGTGTTTGGGTGATGATGAATAAACGGACAAGGCGATTGTCCA

AACTTCCTGAAGAAGTTAGAGGGGAAATAGGGCCTTATTTTATAGAAGATGTTGCTATCA

TAGAGGAGGACAACAGGAAACTACAGAAGCTCAATGAAAACACTGCTGATAATGTTCGA

AGGGGTTTGACTCCTCGCTGGAGTGATCTGGATGTTAATCAGCATGTGAACAATGTCAAAT

ACATTGGTTGGATTCTTGAGAGTGCACCAGGATCCATCTTGGAGAGTCATGAGCTTTCCTG

CATGACCCTTGAATACAGGAGAGAATGTGGGAAGGACAGTGTGCTGCAGTCAATGACTGC

TGTCTCTGGTGGAGGCAGTGCAGCAGGTGGCTCACCAGAATCTAGCGTTGAGTGTGACCA

CTTGCTCCAGCTAGAGAGTGGGCCTGAAGTTGTGAGGGGAAGAACCGAGTGGAGGCCCA

AGAGTGCTAATAACTCGAGGAGCATCCTGGAGATGCCGGCCGAGAGC SEQ ID NO: 9

Cinnamomum camphora (Cc) FATB4 coding DNA sequence codon optimized for Prototheca moriformis

ATGGTGGCCACCGCCGCCGCCTCCGCCTTCTTCCCCGTGGGCGCCCCCGCCACCTCCTCCG

CCACCTCCGCCAAGGCCTCCATGATGCCCGACAACCTGGACGCCCGCGGCATCAAGCCCA

AGCCCGCCTCCTCCTCCGGCCTGCAGGTGAAGGCCAACGCCCACGCCTCCCCCAAGATCA

ACGGCTCCAAGGTGTCCACCGACACCCTGAAGGGCGAGGACACCCTGACCTCCTCCCCCG

CCCCCCGCACCTTCATCAACCAGCTGCCCGACTGGTCCATGTTCCTGGCCGCCATCACCAC

CATCTTCCTGGCCGCCGAGAAGCAGTGGACCAACCTGGACTGGAAGCCCCGCCGCCCCGA

CATGCTGGCCGACCCCTTCGGCATCGGCCGCTTCATGCAGGACGGCCTGATCTTCCGCCAG

C ACTT CGCC AT CCGCT CCT ACGAGATCGGCGCCGACCGC ACCGCCTCC ATCGAGACCCT GA

TGAACCACCTGCAGGAGACCGCCCTGAACCACGTGCGCTCCGCCGGCCTGCTGGGCGACG

GCTTCGGCGCCACCCCCGAGATGTCCCGCCGCGACCTGATCTGGGTGGTGACCCGCATGC

AGGTGCTGGTGGACCGCTACCCCGCCTGGGGCGACATCGTGGAGGTGGAGACCTGGGTGG

GCGCCTCCGGCAAGAACGGCATGCGCCGCGACTGGCTGGTGCGCGACTCCCAGACCGGCG

AGAT CCT GACCCGCGCC ACCT CCGTGT GGGTGAT GAT GAAC AAGCGC ACCCGCCGCCT GT

CCAAGCTGCCCGAGGAGGTGCGCGGCGAGATCGGCCCCTACTTCATCGAGGACGTGGCCA

TCATCGAGGAGGACAACCGCAAGCTGCAGAAGCTGAACGAGAACACCGCCGACAACGTG

CGCCGCGGCCTGACCCCCCGCTGGTCCGACCTGGACGTGAACCAGCACGTGAACAACGTG

AAGTACATCGGCTGGATCCTGGAGTCCGCCCCCGGCTCCATCCTGGAGTCCCACGAGCTGT

CCTGCATGACCCTGGAGTACCGCCGCGAGTGCGGCAAGGACTCCGTGCTGCAGTCCATGA

CCGCCGTGTCCGGCGGCGGCTCCGCCGCCGGCGGCTCCCCCGAGTCCTCCGTGGAGTGCG

ACCACCTGCTGCAGCTGGAGTCCGGCCCCGAGGTGGTGCGCGGCCGCACCGAGTGGCGCC

CCAAGTCCGCCAACAACTCCCGCTCCATCCTGGAGATGCCCGCCGAGTCCCTGTGA SEQ ID NO: 10

Cuphea hyssopifolia (Chs) FATB1 amino acid sequence

MVATNAAAFSAYTFFLTSPTHGYSSKRLADTQNGYPGTSLKSKSTPPPAAAAARNGALPLLAS

ICKCPKKADGSMQLDSSLVFGFQFYIRSYEVGADQTVSIQTVLNYLQEAAINHVQSAGYFGDS

FGATPEMTKRNLIWVITKMQVLVDRYPAWGDVVQVDTWTCSSGKNSMQRDWFVRDLKTGD

IITRASSVWVLMNRLTRKLSKIPEAVLEEAKLFVMNTAPTVDDNRKLPKLDGSSADYVLSGLT

PRWSDLDMNQHVNNVKYIAWILESVPQSIPETHKLSAITVEYRRECGKNSVLQSLTNVSGDGI TCGNSIIECHHLLQLETGPEILLARTEWISKEPGFRGAPIQAEKVYNNK* SEQ ID NO: 11

Cuphea hyssopifolia (Chs) FATB1 coding DNA sequence

ATGGTTGCCACTAATGCTGCTGCCTTTTCTGCTTATACTTTCTTCCTTACTTCACCAACTCA

TGGTTACTCTTCCAAACGTCTCGCCGATACTCAAAATGGTTATCCGGGTACCTCCTTGAAA

TCGAAATCCACTCCTCCACCAGCTGCTGCTGCTGCTCGTAACGGTGCATTGCCACTGCTGG

CCTCCATCTGCAAATGCCCCAAAAAGGCTGATGGGAGTATGCAACTAGACAGCTCCTTGG

TCTTCGGGTTTCAATTTTACATTAGATCATATGAAGTGGGTGCGGATCAAACCGTGTCAAT

ACAGACAGTACTCAATTACTTACAGGAGGCAGCCATCAATCATGTTCAGAGTGCTGGCTA

TTTTGGTGATAGTTTTGGCGCCACCCCGGAAATGACCAAGAGGAACCTCATCTGGGTTATC

ACTAAGATGCAGGTTTTGGTGGATCGCTATCCCGCTTGGGGCGATGTTGTTCAAGTTGATA

CATGGACCTGTAGTTCTGGTAAAAACAGCATGCAGCGTGATTGGTTCGTACGGGATCTCA

AAACTGGAGATATTATAACAAGAGCCTCGAGCGTGTGGGTGCTGATGAATAGACTCACCA

GAAAATTATCAAAAATTCCTGAAGCAGTTCTGGAAGAAGCAAAACTTTTTGTGATGAACA

CTGCCCCCACCGTAGATGACAACAGGAAGCTACCAAAGCTGGATGGCAGCAGTGCTGATT

ATGTCCTCTCTGGCTTAACTCCTAGATGGAGCGACTTAGATATGAACCAGCATGTCAACAA

TGTGAAGTACATAGCCTGGATCCTTGAGAGTGTCCCTCAGAGCATACCGGAGACACACAA

GCTGTCAGCGATAACCGTGGAGTACAGGAGAGAATGTGGCAAGAACAGCGTCCTCCAGTC

TCTGACCAACGTCTCCGGGGATGGAATCACATGTGGAAACAGTATTATCGAGTGCCACCA

TTTGCTTCAACTTGAGACTGGCCCAGAGATTCTACTAGCGCGGACGGAGTGGATATCCAA

GGAACCTGGGTTCAGGGGAGCTCCAATCCAGGCAGAGAAAGTCTACAACAACAAATAA SEQ ID NO: 12

Cuphea hyssopifolia (Chs) FATB1 coding DNA sequence codon optimized for Prototheca moriformis

AT GGTGGCC ACC AACGCCGCCGCCTT CT CCGCCT AC ACCTT CTT CCT G ACCT CCCCC ACCC

ACGGCTACTCCTCCAAGCGCCTGGCCGACACCCAGAACGGCTACCCCGGCACCTCCCTGA

AGTCCAAGTCCACCCCCCCCCCCGCCGCCGCCGCCGCCCGCAACGGCGCCCTGCCCCTGCT

GGCCTCCATCTGCAAGTGCCCCAAGAAGGCCGACGGCTCCATGCAGCTGGACTCCTCCCT

GGTGTTCGGCTTCCAGTTCTACATCCGCTCCTACGAGGTGGGCGCCGACCAGACCGTGTCC

ATCCAGACCGTGCTGAACTACCTGCAGGAGGCCGCCATCAACCACGTGCAGTCCGCCGGC

TACTTCGGCGACTCCTTCGGCGCCACCCCCGAGATGACCAAGCGCAACCTGATCTGGGTG

ATCACCAAGATGCAGGTGCTGGTGGACCGCTACCCCGCCTGGGGCGACGTGGTGCAGGTG

GACACCTGGACCTGCTCCTCCGGCAAGAACTCCATGCAGCGCGACTGGTTCGTGCGCGAC

CTGAAGACCGGCGACATCATCACCCGCGCCTCCTCCGTGTGGGTGCTGATGAACCGCCTG

ACCCGCAAGCTGTCCAAGATCCCCGAGGCCGTGCTGGAGGAGGCCAAGCTGTTCGTGATG

AACACCGCCCCCACCGTGGACGACAACCGCAAGCTGCCCAAGCTGGACGGCTCCTCCGCC

GACTACGTGCTGTCCGGCCTGACCCCCCGCTGGTCCGACCTGGACATGAACCAGCACGTG

AACAACGTGAAGTACATCGCCTGGATCCTGGAGTCCGTGCCCCAGTCCATCCCCGAGACC

CACAAGCTGTCCGCCATCACCGTGGAGTACCGCCGCGAGTGCGGCAAGAACTCCGTGCTG

CAGTCCCTGACCAACGTGTCCGGCGACGGCATCACCTGCGGCAACTCCATCATCGAGTGC

CACCACCTGCTGCAGCTGGAGACCGGCCCCGAGATCCTGCTGGCCCGCACCGAGTGGATC

TCCAAGGAGCCCGGCTTCCGCGGCGCCCCCATCCAGGCCGAGAAGGTGTACAACAACAAG

TGA SEQ ID NO: 13

Cuphea hyssopifolia (Chs) FATB2 amino acid sequence

MVATAASSAFFPVPSPDASSRPGKLGNGSSSLSPLKPKLMANGGLQVKANASAPPKINGSSVG

LKSGSLKTQEDTPSAPPPRTFINQLPDWSMLLAAITTVFLAAEKQWMMLDWKPKRPDMLVDP

FGLGRIVQDGLVFRQNFSIRSYEIGADRTASIETVMNHLQETALNHVKSAGLLNDGFGRTLEM

YKRDLIWWAKMQVMVNRYPTWGDTVEVNTWVAKSGKNGMRRDWLISDCNTGEILTRASS

VWVMMNQKTRRLSKIPDEVRHEIEPHFVDSAPVIEDDDRKLPKLDEKTADSIRKGLTPKWNDL

DVNQHVNNVKYIGWILESTPPEVLETQELCSLTLEYRRECGRESVLESLTAVDPSGKGSGSQFQ HLLRLEDGGEIVKGRTEWRPKTAGINGPIASGETSPGDSS* SEQ ID NO: 14

Cuphea hyssopifolia (Chs) FATB2 coding DNA sequence

ATGGTGGCTACCGCTGCAAGTTCAGCATTCTTCCCTGTGCCGTCCCCCGACGCCTCCTCTA

GACCTGGAAAGCTCGGCAATGGGTCATCGAGCTTGAGCCCCCTCAAGCCCAAATTGATGG

CCAATGGCGGGTTGCAGGTTAAGGCAAACGCCAGTGCCCCTCCTAAGATCAATGGTTCTT

CGGTCGGTCTAAAGTCCGGCAGTCTCAAGACTCAGGAAGACACTCCTTCGGCGCCTCCTCC

CCGGACTTTTATTAACCAGCTGCCTGATTGGAGTATGCTTCTTGCTGCAATCACTACTGTCT

TCTTGGCAGCAGAGAAGCAGTGGATGATGCTTGATTGGAAACCCAAGAGGCCTGACATGC

TTGTGGACCCGTTCGGATTGGGAAGGATTGTTCAAGATGGGCTTGTGTTCAGGCAGAATTT

TTCGATTAGGTCCTATGAAATAGGCGCTGATCGCACTGCGTCTATAGAGACGGTGATGAA

CCACTTGCAGGAAACAGCTCTCAATCATGTTAAGAGTGCTGGGCTTCTTAATGACGGCTTT

GGTCGTACTCTTGAGATGTATAAAAGGGACCTTATTTGGGTTGTTGCAAAAATGCAGGTCA

TGGTTAACCGCTATCCTACTTGGGGCGACACGGTTGAAGTGAATACTTGGGTTGCCAAGTC

AGGGAAAAATGGTATGCGTCGTGATTGGCTCATAAGTGATTGCAATACAGGAGAAATTCT

TACTAGAGCATCAAGTGTGTGGGTCATGATGAATCAAAAGACAAGAAGATTGTCAAAAAT

TCCAGATGAGGTTCGACATGAGATAGAGCCTCATTTCGTGGACTCTGCTCCCGTCATTGAA

GATGATGACCGGAAACTTCCCAAGCTGGATGAGAAGACTGCTGACTCCATCCGCAAGGGT

CTAACTCCGAAGTGGAATGACTTGGATGTCAATCAGCACGTCAACAACGTGAAGTACATT

GGGTGGATTCTTGAGAGTACTCCACCAGAAGTTCTGGAGACCCAGGAGTTATGTTCCCTTA

CCCTGGAATATAGGCGGGAATGCGGAAGGGAGAGCGTGCTGGAGTCCCTCACTGCTGTGG

ACCCCTCTGGAAAGGGCTCTGGGTCTCAGTTCCAGCACCTTCTGCGGCTTGAGGATGGAG

GTGAGATTGTGAAGGGGAGAACTGAGTGGCGACCCAAGACTGCAGGAATCAATGGGCCA

ATAGCATCCGGGGAGACCTCACCTGGAGACTCTTCTTAG SEQ ID NO: 15

Cuphea hyssopifolia (Chs) FATB2 coding DNA sequence codon optimized for Prototheca moriformis

ATGGTGGCCACCGCCGCCTCCTCCGCCTTCTTCCCCGTGCCCTCCCCCGACGCCTCCTCCCG

CCCCGGCAAGCTGGGCAACGGCTCCTCCTCCCTGTCCCCCCTGAAGCCCAAGCTGATGGCC

AACGGCGGCCTGCAGGTGAAGGCCAACGCCTCCGCCCCCCCCAAGATCAACGGCTCCTCC

GTGGGCCTGAAGTCCGGCTCCCTGAAGACCCAGGAGGACACCCCCTCCGCCCCCCCCCCC

CGCACCTTCATCAACCAGCTGCCCGACTGGTCCATGCTGCTGGCCGCCATCACCACCGTGT

TCCTGGCCGCCGAGAAGCAGTGGATGATGCTGGACTGGAAGCCCAAGCGCCCCGACATGC

TGGTGGACCCCTTCGGCCTGGGCCGCATCGTGCAGGACGGCCTGGTGTTCCGCCAGAACTT

CTCCATCCGCTCCTACGAGATCGGCGCCGACCGCACCGCCTCCATCGAGACCGTGATGAA

CCACCTGCAGGAGACCGCCCTGAACCACGTGAAGTCCGCCGGCCTGCTGAACGACGGCTT

CGGCCGCACCCTGGAGATGTACAAGCGCGACCTGATCTGGGTGGTGGCCAAGATGCAGGT

GATGGTGAACCGCTACCCCACCTGGGGCGACACCGTGGAGGTGAACACCTGGGTGGCCAA

GTCCGGCAAGAACGGCATGCGCCGCGACTGGCTGATCTCCGACTGCAACACCGGCGAGAT

CCTGACCCGCGCCTCCTCCGTGTGGGTGATGATGAACCAGAAGACCCGCCGCCTGTCCAA

GATCCCCGACGAGGTGCGCCACGAGATCGAGCCCCACTTCGTGGACTCCGCCCCCGTGAT

CGAGGACGACGACCGCAAGCTGCCCAAGCTGGACGAGAAGACCGCCGACTCCATCCGCA

AGGGCCTGACCCCCAAGTGGAACGACCTGGACGTGAACCAGCACGTGAACAACGTGAAG

TACATCGGCTGGATCCTGGAGTCCACCCCCCCCGAGGTGCTGGAGACCCAGGAGCTGTGC

TCCCTGACCCTGGAGTACCGCCGCGAGTGCGGCCGCGAGTCCGTGCTGGAGTCCCTGACC

GCCGTGGACCCCTCCGGCAAGGGCTCCGGCTCCCAGTTCCAGCACCTGCTGCGCCTGGAG

GACGGCGGCGAGATCGTGAAGGGCCGCACCGAGTGGCGCCCCAAGACCGCCGGCATCAA

CGGCCCCATCGCCTCCGGCGAGACCTCCCCCGGCGACTCCTCCTGA SEQ ID NO: 16

Cuphea hyssopifolia (Chs) FATB2b +a.a.248-259 variant amino acid sequence

MVATAASSAFFPVPSPDASSRPGKLGNGSSSLSPLKPKLMANGGLQVKANASAPPKINGSSVG

LKSGSLKTQEDTPSAPPPRTFINQLPDWSMLLAAITTVFLAAEKQWMMLDWKPKRPDMLVDP

FGLGRIVQDGLVFRQNFSIRSYEIGADRTASIETVMNHLQETALNHVKSAGLLNDGFGRTLEM

YKRDLIWWAKMQVMVNRYPTWGDTVEVNTWVAKSGKNGMRRDWLISDCNTGEILTRASS

KSQIMLPLHYCSVWVMMNQKTRRLSKIPDEVRHEIEPHFVDSAPVIEDDDRKLPKLDEKTADS

IRKGLTPKWNDLDVNQHVNNVKYIGWILESTPPEVLETQELCSLTLEYRRECGRESVLESLTA VDPSGKGSGSQFQHLLRLEDGGEIVKGRTEWRPKTAGINGPIASGETSPGDSS* SEQ ID NO: 17

Cuphea hyssopifolia (Chs) FATB2b+a.a.248-259 variant coding DNA sequence

ATGGTGGCTACCGCTGCAAGTTCAGCATTCTTCCCTGTGCCGTCCCCCGACGCCTCCTCTA

GACCTGGAAAGCTCGGCAATGGGTCATCGAGCTTGAGCCCCCTCAAGCCCAAATTGATGG

CCAATGGCGGGTTGCAGGTTAAGGCAAACGCCAGTGCCCCTCCTAAGATCAATGGTTCTT

CGGTCGGTCTAAAGTCCGGCAGTCTCAAGACTCAGGAAGACACTCCTTCGGCGCCTCCTCC

CCGGACTTTTATTAACCAGCTGCCTGATTGGAGTATGCTTCTTGCTGCAATCACTACTGTCT

TCTTGGCAGCAGAGAAGCAGTGGATGATGCTTGATTGGAAACCCAAGAGGCCTGACATGC

TTGTGGACCCGTTCGGATTGGGAAGGATTGTTCAAGATGGGCTTGTGTTCAGGCAGAATTT

TTCGATTAGGTCCTATGAAATAGGCGCTGATCGCACTGCGTCTATAGAGACGGTGATGAA

CCACTTGCAGGAAACAGCTCTCAATCATGTTAAGAGTGCTGGGCTTCTTAATGACGGCTTT

GGTCGTACTCTTGAGATGTATAAAAGGGACCTTATTTGGGTTGTTGCAAAAATGCAGGTCA

TGGTTAACCGCTATCCTACTTGGGGCGACACGGTTGAAGTGAATACTTGGGTTGCCAAGTC

AGGGAAAAATGGTATGCGTCGTGATTGGCTCATAAGTGATTGCAATACAGGAGAAATTCT

TACTAGAGCATCAAGTAAAAGCCAAATTATGTTACCCTTACATTATTGCAGTGTGTGGGTC

ATGATGAATCAAAAGACAAGAAGATTGTCAAAAATTCCAGATGAGGTTCGACATGAGATA

GAGCCTCATTTCGTGGACTCTGCTCCCGTCATTGAAGATGATGACCGGAAACTTCCCAAGC

TGGATGAGAAGACTGCTGACTCCATCCGCAAGGGTCTAACTCCGAAGTGGAATGACTTGG

ATGTCAATCAGCACGTCAACAACGTGAAGTACATTGGGTGGATTCTTGAGAGTACTCCAC

CAGAAGTTCTGGAGACCCAGGAGTTATGTTCCCTTACCCTGGAATATAGGCGGGAATGCG

GAAGGGAGAGCGTGCTGGAGTCCCTCACTGCTGTGGACCCCTCTGGAAAGGGCTCTGGGT

CTCAGTTCCAGCACCTTCTGCGGCTTGAGGATGGAGGTGAGATTGTGAAGGGGAGAACTG

AGTGGCGACCCAAGACTGCAGGAATCAATGGGCCAATAGCATCCGGGGAGACCTCACCTG

GAGACTCTTCTTAG SEQ ID NO: 18

Cuphea hyssopifolia (Chs) FATB2b +a.a.248-259 variant coding DNA sequence codon optimized for Prototheca moriformis

ATGGTGGCCACCGCCGCCTCCTCCGCCTTCTTCCCCGTGCCCTCCCCCGACGCCTCCTCCCG

CCCCGGCAAGCTGGGCAACGGCTCCTCCTCCCTGTCCCCCCTGAAGCCCAAGCTGATGGCC

AACGGCGGCCTGCAGGTGAAGGCCAACGCCTCCGCCCCCCCCAAGATCAACGGCTCCTCC

GTGGGCCTGAAGTCCGGCTCCCTGAAGACCCAGGAGGACACCCCCTCCGCCCCCCCCCCC

CGCACCTTCATCAACCAGCTGCCCGACTGGTCCATGCTGCTGGCCGCCATCACCACCGTGT

TCCTGGCCGCCGAGAAGCAGTGGATGATGCTGGACTGGAAGCCCAAGCGCCCCGACATGC

TGGTGGACCCCTTCGGCCTGGGCCGCATCGTGCAGGACGGCCTGGTGTTCCGCCAGAACTT

CTCCATCCGCTCCTACGAGATCGGCGCCGACCGCACCGCCTCCATCGAGACCGTGATGAA

CCACCTGCAGGAGACCGCCCTGAACCACGTGAAGTCCGCCGGCCTGCTGAACGACGGCTT

CGGCCGCACCCTGGAGATGTACAAGCGCGACCTGATCTGGGTGGTGGCCAAGATGCAGGT

GATGGTGAACCGCTACCCCACCTGGGGCGACACCGTGGAGGTGAACACCTGGGTGGCCAA

GTCCGGCAAGAACGGCATGCGCCGCGACTGGCTGATCTCCGACTGCAACACCGGCGAGAT

CCTGACCCGCGCCTCCTCCAAGTCCCAGATCATGCTGCCCCTGCACTACTGCTCCGTGTGG

GTGATGATGAACCAGAAGACCCGCCGCCTGTCCAAGATCCCCGACGAGGTGCGCCACGAG

ATCGAGCCCCACTTCGTGGACTCCGCCCCCGTGATCGAGGACGACGACCGCAAGCTGCCC

AAGCTGGACGAGAAGACCGCCGACTCCATCCGCAAGGGCCTGACCCCCAAGTGGAACGA

CCTGGACGTGAACCAGCACGTGAACAACGTGAAGTACATCGGCTGGATCCTGGAGTCCAC

CCCCCCCGAGGTGCTGGAGACCCAGGAGCTGTGCTCCCTGACCCTGGAGTACCGCCGCGA

GTGCGGCCGCGAGTCCGTGCTGGAGTCCCTGACCGCCGTGGACCCCTCCGGCAAGGGCTC

CGGCTCCCAGTTCCAGCACCTGCTGCGCCTGGAGGACGGCGGCGAGATCGTGAAGGGCCG

CACCGAGTGGCGCCCCAAGACCGCCGGCATCAACGGCCCCATCGCCTCCGGCGAGACCTC

CCCCGGCGACTCCTCCTGA SEQ ID NO: 19

Cuphea hyssopifolia (Chs) FATB3 amino acid sequence

MVAAEASSALFSVRTPGTSPKPGKFGNWPTSLSVPFKSKSNHNGGFQVKANASARPKANGSA

V SLKSGSLDTQEDTS S S S SPPRTFINQLPD W SMLLSAITTVFVAAEKQWTMLDRKSKRPDMLM

DPFGVDRWQDGAVFRQSFSIRSYEIGADRTASIETLMNIFQETSLNHCKSIGLLNDGFGRTPEM

CKRDLIWWTKMHVEVNRYPTWGDTIEVNTWVSESGKTGMGRDWLISDCHTGEILIRATSMC

AMMNQKTRRFSKFPYEVRQELAPHFVDSAPVIEDYQKLHKLDVKTGDSICNGLTPRWNDLDV

NQHVNNVKYIGWILESVPTEVFETQELCGLTLEYRRECGRDSVLESVTAMDPSKEGDRSLYQH LLRLEDGADIAKGRTKWRPKNAGTNGAISTGKTSNGNSIS* SEQ ID NO: 20

Cuphea hyssopifolia (Chs) FATB3 coding DNA sequence

ATGGTGGCTGCCGAAGCAAGTTCTGCACTCTTCTCCGTTCGAACCCCGGGAACCTCCCCTA

AACCCGGGAAGTTCGGGAATTGGCCAACGAGCTTGAGCGTCCCCTTCAAGTCCAAATCAA

ACCACAATGGCGGCTTTCAGGTTAAGGCAAACGCCAGTGCCCGTCCTAAGGCTAACGGTT

CTGCAGTAAGTCTAAAGTCTGGCAGCCTCGACACTCAGGAGGACACTTCATCGTCGTCCTC

TCCTCCTCGGACTTTCATTAACCAGTTGCCCGACTGGAGTATGCTGCTGTCCGCGATCACG

ACCGTCTTCGTGGCGGCTGAGAAGCAGTGGACGATGCTTGATCGGAAATCTAAGAGGCCC

GACATGCTCATGGACCCGTTTGGGGTTGACAGGGTTGTTCAGGATGGGGCTGTGTTCAGA

CAGAGTTTTTCGATTAGGTCTTACGAAATAGGCGCTGATCGAACAGCCTCTATAGAGACG

CTGATGAACATCTTCCAGGAAACATCTCTCAATCATTGTAAGAGTATCGGTCTTCTCAATG

ACGGCTTTGGTCGTACTCCTGAGATGTGTAAGAGGGACCTCATTTGGGTGGTTACAAAAAT

GCACGTCGAGGTTAATCGCTATCCTACTTGGGGTGATACTATCGAGGTCAATACTTGGGTC

TCCGAGTCGGGGAAAACCGGTATGGGTCGTGATTGGCTGATAAGTGATTGTCATACAGGA

GAAATTCTAATAAGAGCAACGAGCATGTGTGCTATGATGAATCAAAAGACGAGAAGATTC

TCAAAATTTCCATATGAGGTTCGACAGGAGTTGGCGCCTCATTTTGTGGACTCTGCTCCTG

TCATTGAAGACTATCAAAAATTGCACAAGCTTGATGTGAAGACGGGTGATTCCATTTGCA

ATGGCCTAACTCCAAGGTGGAATGACTTGGATGTCAATCAGCACGTTAACAATGTGAAGT

ACATTGGGTGGATTCTCGAGAGTGTTCCAACGGAAGTTTTCGAGACCCAGGAGCTATGTG

GCCTCACCCTTGAGTATAGGCGGGAATGCGGAAGGGACAGTGTGCTGGAGTCCGTGACCG

CTATGGATCCATCAAAAGAGGGAGACAGATCTCTGTACCAGCACCTTCTTCGGCTTGAGG

ATGGGGCTGATATCGCGAAGGGCAGAACCAAGTGGCGGCCGAAGAATGCAGGAACCAAT

GGGGCAATATCAACAGGAAAGACTTCAAATGGAAACTCGATCTCTTAG SEQ ID NO: 21

Cuphea hyssopifolia (Chs) FATB3 coding DNA sequence codon optimized for Prototheca moriformis

AT GGTGGCCGCCGAGGCCT CCTCCGCCCT GTT CT CCGTGCGC ACCCCCGGC ACCT CCCCC A

AGCCCGGCAAGTTCGGCAACTGGCCCACCTCCCTGTCCGTGCCCTTCAAGTCCAAGTCCAA

CCACAACGGCGGCTTCCAGGTGAAGGCCAACGCCTCCGCCCGCCCCAAGGCCAACGGCTC

CGCCGTGTCCCTGAAGTCCGGCTCCCTGGACACCCAGGAGGACACCTCCTCCTCCTCCTCC

CCCCCCCGCACCTTCATCAACCAGCTGCCCGACTGGTCCATGCTGCTGTCCGCCATCACCA

CCGTGTTCGTGGCCGCCGAGAAGCAGTGGACCATGCTGGACCGCAAGTCCAAGCGCCCCG

ACATGCTGATGGACCCCTTCGGCGTGGACCGCGTGGTGCAGGACGGCGCCGTGTTCCGCC

AGTCCTTCTCCATCCGCTCCTACGAGATCGGCGCCGACCGCACCGCCTCCATCGAGACCCT

GATGAACATCTTCCAGGAGACCTCCCTGAACCACTGCAAGTCCATCGGCCTGCTGAACGA

CGGCTTCGGCCGCACCCCCGAGATGTGCAAGCGCGACCTGATCTGGGTGGTGACCAAGAT

GCACGTGGAGGTGAACCGCTACCCCACCTGGGGCGACACCATCGAGGTGAACACCTGGGT

GTCCGAGTCCGGCAAGACCGGCATGGGCCGCGACTGGCTGATCTCCGACTGCCACACCGG

CGAGATCCTGATCCGCGCCACCTCCATGTGCGCCATGATGAACCAGAAGACCCGCCGCTT

CTCCAAGTTCCCCTACGAGGTGCGCCAGGAGCTGGCCCCCCACTTCGTGGACTCCGCCCCC

GTGATCGAGGACTACCAGAAGCTGCACAAGCTGGACGTGAAGACCGGCGACTCCATCTGC

AACGGCCTGACCCCCCGCTGGAACGACCTGGACGTGAACCAGCACGTGAACAACGTGAA

GTACATCGGCTGGATCCTGGAGTCCGTGCCCACCGAGGTGTTCGAGACCCAGGAGCTGTG

CGGCCTGACCCTGGAGTACCGCCGCGAGTGCGGCCGCGACTCCGTGCTGGAGTCCGTGAC

CGCCATGGACCCCTCCAAGGAGGGCGACCGCTCCCTGTACCAGCACCTGCTGCGCCTGGA

GGACGGCGCCGACATCGCCAAGGGCCGCACCAAGTGGCGCCCCAAGAACGCCGGCACCA

ACGGCGCCATCTCCACCGGCAAGACCTCCAACGGCAACTCCATCTCCTGA SEQ ID NO: 22

Cuphea hyssopifolia (Chs) FATB3b (V204I,C239F, E243D, M251V variant) amino acid sequence

MVAAEASSALFSVRTPGTSPKPGKFGNWPTSLSVPFKSKSNHNGGFQVKANASARPKANGSA

VSLKSGSLDTQEDTSSSSSPPRTFINQLPDWSMLLSAITTVFVAAEKQWTMLDRKSKRPDMLM

DPFGVDRWQDGAVFRQSFSIRSYEIGADRTASIETLMNIFQETSLNHCKSIGLLNDGFGRTPEM

CKRDLIWWTKMHIEVNRYPTWGDTIEVNTWVSESGKTGMGRDWLISDFHTGDILIRATSVC

AMMNQKTRRFSKFPYEVRQELAPHFVDSAPVIEDYQKLHKLDVKTGDSICNGLTPRWNDLDV

NQHVNNVKYIGWILESVPTEVFETQELCGLTLEYRRECGRDSVLESVTAMDPSKEGDRSLYQH LLRLEDGADIAKGRTKWRPKNAGTNGAISTGKTSNGNSIS* SEQ ID NO: 23

Cuphea hyssopifolia (Chs) FATB3b (V204I,C239F, E243D, M251V variant) coding DNA sequence

ATGGTGGCTGCCGAAGCAAGTTCTGCACTCTTCTCCGTTCGAACCCCGGGAACCTCCCCTA

AACCCGGGAAGTTCGGGAATTGGCCAACGAGCTTGAGCGTCCCCTTCAAGTCCAAATCAA

ACCACAATGGCGGCTTTCAGGTTAAGGCAAACGCCAGTGCCCGTCCTAAGGCTAACGGTT

CTGCAGTAAGTCTAAAGTCTGGCAGCCTCGACACTCAGGAGGACACTTCATCGTCGTCCTC

TCCTCCTCGGACTTTCATTAACCAGTTGCCCGACTGGAGTATGCTGCTGTCCGCGATCACG

ACCGTCTTCGTGGCGGCTGAGAAGCAGTGGACGATGCTTGATCGGAAATCTAAGAGGCCC

GACATGCTCATGGACCCGTTTGGGGTTGACAGGGTTGTTCAGGATGGGGCTGTGTTCAGA

CAGAGTTTTTCGATTAGGTCTTACGAAATAGGCGCTGATCGAACAGCCTCTATAGAGACG

CTGATGAACATCTTCCAGGAAACATCTCTCAATCATTGTAAGAGTATCGGTCTTCTCAATG

ACGGCTTTGGTCGTACTCCTGAGATGTGTAAGAGGGACCTCATTTGGGTGGTTACAAAAAT

GCACATCGAGGTTAATCGCTATCCTACTTGGGGTGATACTATCGAGGTCAATACTTGGGTC

TCCGAGTCGGGGAAAACCGGTATGGGTCGTGATTGGCTGATAAGTGATTTTCATACAGGA

GACATTCTAATAAGAGCAACGAGCGTGTGTGCTATGATGAATCAAAAGACGAGAAGATTC

TCAAAATTTCCATATGAGGTTCGACAGGAGTTAGCGCCTCATTTTGTGGACTCTGCTCCAG

TCATTGAAGACTATCAAAAATTGCACAAGCTTGATGTGAAGACGGGTGATTCCATTTGCA

ATGGCCTAACTCCAAGGTGGAATGACTTGGATGTCAATCAGCACGTTAACAATGTGAAGT

AC ATT GGGT GGATTCTCGAGAGT GTT CC AACGGAAGTTTTCGAGACCC AGGAGCTAT GTG

GCCTCACCCTTGAGTATAGGCGGGAATGCGGAAGGGACAGTGTGCTGGAGTCCGTGACCG

CTATGGATCCCTCAAAAGAGGGAGACAGATCTCTGTACCAGCACCTTCTTCGGCTTGAGG

ATGGGGCTGATATCGCGAAGGGCAGAACCAAGTGGCGGCCGAAGAATGCAGGAACCAAT

GGGGCAATATCAACAGGAAAGACTTCAAATGGAAACTCGATCTCTTAG SEQ ID NO: 24

Cuphea hyssopifolia (Chs) FATB3b (V204I,C239F, E243D, M251V variant) coding DNA sequence codon optimized for Prototheca moriformis

ATGGTGGCCGCCGAGGCCTCCTCCGCCCTGTTCTCCGTGCGCACCCCCGGCACCTCCCCCA AGCCCGGCAAGTTCGGCAACTGGCCCACCTCCCTGTCCGTGCCCTTCAAGTCCAAGTCCAA CCACAACGGCGGCTTCCAGGTGAAGGCCAACGCCTCCGCCCGCCCCAAGGCCAACGGCTC CGCCGTGTCCCTGAAGTCCGGCTCCCTGGACACCCAGGAGGACACCTCCTCCTCCTCCTCC CCCCCCCGC ACCTT CAT CAACCAGCTGCCCGACT GGTCCATGCTGCT GTCCGCC ATC ACC A CCGTGTTCGT GGCCGCCGAGAAGCAGTGGACC AT GCT GGACCGC AAGTCC AAGCGCCCCG

ACATGCTGATGGACCCCTTCGGCGTGGACCGCGTGGTGCAGGACGGCGCCGTGTTCCGCC

AGTCCTTCTCCATCCGCTCCTACGAGATCGGCGCCGACCGCACCGCCTCCATCGAGACCCT

GATGAACATCTTCCAGGAGACCTCCCTGAACCACTGCAAGTCCATCGGCCTGCTGAACGA

CGGCTTCGGCCGCACCCCCGAGATGTGCAAGCGCGACCTGATCTGGGTGGTGACCAAGAT

GCACATCGAGGTGAACCGCTACCCCACCTGGGGCGACACCATCGAGGTGAACACCTGGGT

GTCCGAGTCCGGCAAGACCGGCATGGGCCGCGACTGGCTGATCTCCGACTTCCACACCGG

CGACATCCTGATCCGCGCCACCTCCGTGTGCGCCATGATGAACCAGAAGACCCGCCGCTT

CTCC AAGTTCCCCT ACGAGGT GCGCC AGGAGCT GGCCCCCCACTTCGT GGACTCCGCCCCC

GTGATCGAGGACTACCAGAAGCTGCACAAGCTGGACGTGAAGACCGGCGACTCCATCTGC

AACGGCCTGACCCCCCGCTGGAACGACCTGGACGTGAACCAGCACGTGAACAACGTGAA

GTACATCGGCTGGATCCTGGAGTCCGTGCCCACCGAGGTGTTCGAGACCCAGGAGCTGTG

CGGCCTGACCCTGGAGTACCGCCGCGAGTGCGGCCGCGACTCCGTGCTGGAGTCCGTGAC

CGCCATGGACCCCTCCAAGGAGGGCGACCGCTCCCTGTACCAGCACCTGCTGCGCCTGGA

GGACGGCGCCGACATCGCCAAGGGCCGCACCAAGTGGCGCCCCAAGAACGCCGGCACCA

ACGGCGCCATCTCCACCGGCAAGACCTCCAACGGCAACTCCATCTCCTGA SEQ ID NO: 25

Cuphea PSR23 (Cu) FATB3 amino acid sequence

MWAAATSAFFPVPAPGTSPKPGKSGNWPSSLSPTFKPKSIPNAGFQVKANASAHPKANGSAV

NLKSGSLNTQEDTS SSPPPRAFLNQLPDW SMLLTAITTVFVAAEKQWTMLDRKSKRPDMLVD

SVGLKCIVRDGLVSRQSFLIRSYEIGADRTASIETLMNHLQETSINHCKSLGLLNDGFGRTPGM

CKNDLIWVLTKMQIMVNRYPTWGDTVEINTWFSQSGKIGMASDWLISDCNTGEILIRATSVW

AMMNQKTRRFSRLPYEVRQELTPHFVDSPHVIEDNDQKLHKFDVKTGDSIRKGLTPRWNDLD

VNQHVSNVKYIGWILESMPIEVLETQELCSLTVEYRRECGMDSVLESVTAVDPSENGGRSQYK HLLRLEDGTDIVKSRTEWRPKNAGTNGAISTSTAKTSNGNSVS* SEQ ID NO: 26

Cuphea PSR23 (Cu) FATB3 coding DNA sequence

ATGGTGGTGGCTGCAGCAACTTCTGCATTCTTCCCCGTTCCAGCCCCGGGAACCTCCCCTA

AACCCGGGAAGTCCGGCAACTGGCCATCGAGCTTGAGCCCTACCTTCAAGCCCAAGTCAA

TCCCCAATGCCGGATTTCAGGTTAAGGCAAATGCCAGTGCCCATCCTAAGGCTAACGGTTC

TGCAGTAAATCTAAAGTCTGGCAGCCTCAACACTCAGGAGGACACTTCGTCGTCCCCTCCT

CCCCGGGCTTTCCTTAACCAGTTGCCTGATTGGAGTATGCTTCTGACTGCAATCACGACCG

TCTTCGTGGCGGCAGAGAAGCAGTGGACTATGCTTGATAGGAAATCTAAGAGGCCTGACA

TGCTCGTGGACTCGGTTGGGTTGAAGTGTATTGTTCGGGATGGGCTCGTGTCCAGACAGAG

TTTTTTC , ATT AG ATCTT ATG A A AT A( i( iC( iCTG ATCG A AC AGCCTCT ATAG AGACGCTGATG

AACCACTTGCAGGAAACATCTATCAATCATTGTAAGAGTTTGGGTCTTCTCAATGACGGCT

TTGGTCGTACTCCTGGGATGTGTAAAAACGACCTCATTTGGGTGCTTACAAAAATGCAGAT

CATGGTGAATCGCTACCCAACTTGGGGCGATACTGTTGAGATCAATACCTGGTTCTCTCAG

TCGGGGAAAATCGGTATGGCTAGCGATTGGCTAATAAGTGATTGCAACACAGGAGAAATT

CTTATAAGAGCAACGAGCGTGTGGGCTATGATGAATCAAAAGACGAGAAGATTCTCAAGA

CTTCCATACGAGGTTCGCCAGGAGTTAACGCCTCATTTTGTGGACTCTCCTCATGTCATTG

AAGACAATGATCAGAAATTGCATAAGTTTGATGTGAAGACTGGTGATTCCATTCGCAAGG

GTCTAACTCCGAGGTGGAACGACTTGGATGTGAATCAGCACGTAAGCAACGTGAAGTACA

TT GGGT GGATTCTCGAGAGTATGCCAATAGAAGTTTTGGAGAC AC AGGAGCT AT GCTCTCT

CACCGTAGAATATAGGCGGGAATGCGGAATGGACAGTGTGCTGGAGTCCGTGACTGCTGT

GGATCCCTCAGAAAATGGAGGCCGGTCTCAGTACAAGCACCTTCTGCGGCTTGAGGATGG

GACTGATATCGTGAAGAGCAGAACTGAGTGGCGACCGAAGAATGCAGGAACTAACGGGG

CGATATCAACATCAACAGCAAAGACTTCAAATGGAAACTCGGTCTCTTAG SEQ ID NO: 27

Cuphea PSR23 (Cu) FATB3 coding DNA sequence codon optimized for Prototheca moriformis

AT GGT GGT GGCCGCCGCC ACCT CCGCCTT CTT CCCCGTGCCCGCCCCCGGC ACCTCCCCC A AGCCCGGCAAGTCCGGCAACTGGCCCTCCTCCCTGTCCCCCACCTTCAAGCCCAAGTCCAT CCCCAACGCCGGCTTCCAGGTGAAGGCCAACGCCTCCGCCCACCCCAAGGCCAACGGCTC

CGCCGTGAACCTGAAGTCCGGCTCCCTGAACACCCAGGAGGACACCTCCTCCTCCCCCCCC

CCCCGCGCCTT CCT GAACCAGCT GCCCGACT GGT CC AT GCTGCTGACCGCC AT CACC ACCG

TGTTCGTGGCCGCCGAGAAGCAGTGGACCATGCTGGACCGCAAGTCCAAGCGCCCCGACA

TGCTGGTGGACTCCGTGGGCCTGAAGTGCATCGTGCGCGACGGCCTGGTGTCCCGCCAGT

CCTTCCTGATCCGCTCCTACGAGATCGGCGCCGACCGCACCGCCTCCATCGAGACCCTGAT

GAACC ACCT GC AGGAGACCT CC AT CAACC ACT GCAAGTCCCTGGGCCTGCT GAACGACGG

CTTCGGCCGCACCCCCGGCATGTGCAAGAACGACCTGATCTGGGTGCTGACCAAGATGCA

GAT CAT GGT GAACCGCT ACCCC ACCTGGGGCGAC ACCGTGGAGATC AAC ACCT GGTT CTC

CCAGTCCGGCAAGATCGGCATGGCCTCCGACTGGCTGATCTCCGACTGCAACACCGGCGA

GATCCTGATCCGCGCCACCTCCGTGTGGGCCATGATGAACCAGAAGACCCGCCGCTTCTCC

CGCCT GCCCT ACGAGGTGCGCC AGGAGCT GACCCCCC ACTT CGT GGACTCCCCCC ACGT G

AT CGAGGAC AACGACCAGAAGCT GC AC AAGTT CGACGTGAAGACCGGCGACTCC AT CCGC

AAGGGCCTGACCCCCCGCTGGAACGACCTGGACGTGAACCAGCACGTGTCCAACGTGAAG

TACATCGGCTGGATCCTGGAGTCCATGCCCATCGAGGTGCTGGAGACCCAGGAGCTGTGC

TCCCTGACCGTGGAGTACCGCCGCGAGTGCGGCATGGACTCCGTGCTGGAGTCCGTGACC

GCCGTGGACCCCTCCGAGAACGGCGGCCGCTCCCAGTACAAGCACCTGCTGCGCCTGGAG

GACGGC ACCGACATCGT GAAGT CCCGC ACCGAGT GGCGCCCCAAGAACGCCGGCACCAA

CGGCGCCATCTCCACCTCCACCGCCAAGACCTCCAACGGCAACTCCGTGTCCTGA SEQ ID NO: 28

Cuphea wrightii (Cw) FATB3 amino acid sequence

MWAAAASSAFFPVPAPRTTPKPGKFGNWPSSLSPPFKPKSNPNGRFQVKANVSPHPKANGSA

V SLKSGSLNTLEDPP S SPPPRTFLNQLPDW SRLRTAITT WVAAEKQFTRLDRKSKRPDMLVDW

FGSETIVQDGLVFRERFSIRSYEIGADRTASIETLMNHLQDTSLNHCKSVGLLNDGFGRTSEMC

TRDLIWVLTKMQIWNRYPTWGDTVEINSWFSQSGKIGMGRDWLISDCNTGEILVRATSAWA

MMNQKTRRFSKLPCEVRQEIAPHFVDAPPVIEDNDRKLHKFDVKTGDSICKGLTPGWNDLDV

NQHVSNVKYIGWILESMPTEVLETQELCSLTLEYRRECGRESWESVTSMNPSKVGDRSQYQH LLRLEDGADIMKGRTEWRPKNAGTNRAIST* SEQ ID NO: 29

Cuphea wrightii (Cw) FATB3 coding DNA sequence

ATGGTGGTGGCTGCTGCAGCAAGTTCTGCATTCTTCCCTGTTCCAGCACCTAGAACCACGC

CTAAACCCGGGAAGTTCGGCAATTGGCCATCGAGCTTGAGCCCGCCCTTCAAGCCCAAGT

CAAACCCCAATGGTAGATTTCAGGTTAAGGCAAATGTCAGTCCTCATCCTAAGGCTAACG

GTTCTGCAGTAAGTCTAAAGTCTGGCAGCCTCAACACTCTGGAGGACCCTCCGTCGTCCCC

TCCTCCTCGGACTTTCCTTAACCAGTTGCCTGATTGGAGTAGGCTTCGGACTGCAATCACG

ACCGTCTTCGTGGCGGCAGAGAAGCAGTTCACTAGGCTCGATCGAAAATCTAAGAGGCCT

GACATGCTAGTGGACTGGTTTGGGTCAGAGACTATTGTTCAGGATGGGCTCGTGTTCAGA

GAGAGATTTTCGATCAGGTCTTACGAAATAGGCGCTGATCGAACAGCCTCTATAGAGACG

CTGATGAACCACTTGCAGGACACATCTCTGAATCATTGTAAGAGTGTGGGTCTTCTCAATG

ACGGCTTTGGTCGTACCTCGGAGATGTGTACAAGAGACCTCATTTGGGTGCTTACAAAAAT

GCAGATCGTGGTGAATCGCTATCCAACTTGGGGCGATACTGTCGAGATCAATAGCTGGTT

CTCCCAGTCGGGGAAAATCGGTATGGGTCGCGATTGGCTAATAAGTGATTGCAACACAGG

AGAAATTCTTGTAAGAGCAACGAGCGCTTGGGCCATGATGAATCAAAAGACGAGAAGATT

CTCAAAACTTCCATGCGAGGTTCGCCAGGAGATAGCGCCTCATTTTGTGGACGCTCCTCCT

GTCATTGAAGACAATGATCGGAAATTGCATAAGTTTGATGTGAAGACTGGTGATTCCATTT

GCAAGGGTCTAACTCCGGGGTGGAATGACTTGGATGTCAATCAGCACGTAAGCAACGTGA

AGTACATTGGGTGGATTCTCGAGAGTATGCCTACAGAAGTTTTGGAGACCCAGGAGCTAT

GCTCTCTCACCCTTGAATATAGGCGGGAATGTGGAAGGGAAAGTGTGGTAGAGTCCGTGA

CCTCTATGAATCCCTCAAAAGTTGGAGACCGGTCTCAGTACCAACACCTTCTGCGGCTTGA

GGATGGGGCTGATATCATGAAGGGCAGAACTGAGTGGAGACCAAAGAATGCAGGAACCA

ACCGGGCGATATCAACATGA SEQ ID NO: 30

Cuphea wrightii (Cw) FATB3 coding DNA sequence codon optimized for Prototheca moriformis

ATGGTGGTGGCCGCCGCCGCCTCCTCCGCCTTCTTCCCCGTGCCCGCCCCCCGCACCACCC

CCAAGCCCGGCAAGTTCGGCAACTGGCCCTCCTCCCTGTCCCCCCCCTTCAAGCCCAAGTC

CAACCCCAACGGCCGCTTCCAGGTGAAGGCCAACGTGTCCCCCCACCCCAAGGCCAACGG

CTCCGCCGTGTCCCTGAAGTCCGGCTCCCTGAACACCCTGGAGGACCCCCCCTCCTCCCCC

CCCCCCCGCACCTTCCTGAACCAGCTGCCCGACTGGTCCCGCCTGCGCACCGCCATCACCA

CCGTGTTCGTGGCCGCCGAGAAGCAGTTCACCCGCCTGGACCGCAAGTCCAAGCGCCCCG

ACATGCTGGTGGACTGGTTCGGCTCCGAGACCATCGTGCAGGACGGCCTGGTGTTCCGCG

AGCGCTTCTCCATCCGCTCCTACGAGATCGGCGCCGACCGCACCGCCTCCATCGAGACCCT

GATGAACCACCTGCAGGACACCTCCCTGAACCACTGCAAGTCCGTGGGCCTGCTGAACGA

CGGCTTCGGCCGCACCTCCGAGATGTGCACCCGCGACCTGATCTGGGTGCTGACCAAGAT

GCAGATCGTGGTGAACCGCTACCCCACCTGGGGCGACACCGTGGAGATCAACTCCTGGTT

CTCCCAGTCCGGCAAGATCGGCATGGGCCGCGACTGGCTGATCTCCGACTGCAACACCGG

CGAGATCCTGGTGCGCGCCACCTCCGCCTGGGCCATGATGAACCAGAAGACCCGCCGCTT

CTCCAAGCTGCCCTGCGAGGTGCGCCAGGAGATCGCCCCCCACTTCGTGGACGCCCCCCC

CGTGATCGAGGACAACGACCGCAAGCTGCACAAGTTCGACGTGAAGACCGGCGACTCCAT

CTGCAAGGGCCTGACCCCCGGCTGGAACGACCTGGACGTGAACCAGCACGTGTCCAACGT

GAAGTACATCGGCTGGATCCTGGAGTCCATGCCCACCGAGGTGCTGGAGACCCAGGAGCT

GT GCT CCCT GACCCT GGAGT ACCGCCGCGAGTGCGGCCGCGAGTCCGTGGTGGAGTCCGT

GACCTCCATGAACCCCTCCAAGGTGGGCGACCGCTCCCAGTACCAGCACCTGCTGCGCCT

GGAGGACGGCGCCGACATCATGAAGGGCCGCACCGAGTGGCGCCCCAAGAACGCCGGCA

CCAACCGCGCCATCTCCACCTGA SEQ ID NO: 31

Cuphea wrightii (Cw) FATB4a amino acid sequence

MVATAASSAFFPVPSADTSSSRPGKLGSGPSSLSPLKPKSIPNGGLQVKANASAPPKINGSSVGL

KSGGFKTQEDSPSAPPPRTFINQLPDWSMLLAAITTVFLAAEKQWMMLDWKPKRPDMLVDPF

GLGSIVQDGLVFRQNFSIRSYEIGADRTASIETVMNHLQETALNHVKIAGLSNDGFGRTPEMYK

RDLIWWAKMQVMVNRYPTWGDTVEVNTWVAKSGKNGMRRDWLISDCNTGEILTRASSVW

VMMNQKTRRLSKIPDEVRNEIEPHFVDSAPWEDDDRKLPKLDENTADSIRKGLTPRWNDLD

VNQHVNNVKYIGWILESTPPEVLETQELCSLTLEYRRECGRESVLESLTAVDPSAEGYASRFQH LLRLEDGGEIVKARTEWRPKNAGINGWPSEESSPGDFF* SEQ ID NO: 32

Cuphea wrightii (Cw) FATB4a coding DNA sequence

TTGGTGGCTACCGCTGCAAGTTCTGCATTTTTCCCCGTGCCATCCGCCGACACCTCCTCCTC

GAGACCCGGAAAGCTCGGCAGTGGACCATCGAGCTTGAGCCCCCTCAAGCCCAAATCGAT

CCCCAATGGCGGCTTGCAGGTTAAGGCAAACGCCAGTGCCCCTCCTAAGATCAATGGTTC

CTCGGTCGGTCTAAAGTCGGGCGGTTTCAAGACTCAGGAAGACTCTCCTTCGGCCCCTCCT

CCGCGGACTTTTATCAACCAGTTGCCTGATTGGAGTATGCTTCTTGCTGCAATCACTACTG

TCTTCTTGGCTGCAGAGAAGCAGTGGATGATGCTTGATTGGAAACCTAAGAGGCCTGACA

TGCTCGTGGACCCGTTCGGATTGGGAAGTATTGTTCAGGATGGGCTTGTGTTCAGGCAGAA

TTTTTCAATTAGGTCCTACGAAATAGGCGCCGATCGAACTGCGTCTATAGAGACGGTGATG

AACCATTTGCAGGAAACAGCTCTCAATCATGTCAAGATTGCTGGGCTTTCTAATGACGGCT

TTGGTCGTACTCCTGAGATGTATAAAAGAGACCTTATTTGGGTTGTTGCAAAAATGCAGGT

CATGGTTAACCGCTATCCTACTTGGGGTGACACGGTTGAAGTGAATACTTGGGTTGCCAAG

TCAGGGAAAAATGGTATGCGTCGTGACTGGCTCATAAGTGATTGCAATACTGGAGAGATT

CTTACAAGAGCATCAAGCGTGTGGGTCATGATGAATCAAAAGACAAGAAGATTGTCAAAA

ATTCCAGATGAGGTTCGAAATGAGATAGAGCCTCATTTTGTGGACTCTGCTCCCGTCGTTG

AAGATGATGATCGGAAACTTCCCAAGCTGGATGAGAACACTGCTGACTCCATCCGCAAGG

GTCTAACTCCGAGGTGGAATGACTTGGATGTCAATCAGCACGTCAACAACGTGAAGTACA

TCGGATGGATTCTTGAGAGTACTCCACCAGAAGTTCTGGAGACCCAGGAGTTATGCTCCCT

GACCCTGGAATACAGGCGGGAATGTGGAAGGGAGAGCGTGCTGGAGTCCCTCACTGCTGT

CGACCCGTCTGCAGAGGGCTATGCGTCCCGGTTTCAGCACCTTCTGCGGCTTGAGGATGGA

GGTGAGATCGTGAAGGCGAGAACTGAGTGGCGACCCAAGAATGCTGGAATCAATGGGGT

GGTACCATCCGAGGAGTCCTCACCTGGAGACTTCTTTTAG SEQ ID NO: 33

Cuphea wrightii (Cw) FATB4a coding DNA sequence codon optimized for Prototheca moriformis

AT GGT GGCC ACCGCCGCCT CCT CCGCCTT CTTCCCCGTGCCCTCCGCCGACACCTCCT CCT C

CCGCCCCGGC AAGCT GGGCTCCGGCCCCT CCT CCCT GTCCCCCCT GAAGCCC AAGT CC ATC

CCCAACGGCGGCCTGCAGGTGAAGGCCAACGCCTCCGCCCCCCCCAAGATCAACGGCTCC

TCCGTGGGCCTGAAGTCCGGCGGCTTCAAGACCCAGGAGGACTCCCCCTCCGCCCCCCCC

CCCCGCACCTTCATCAACCAGCTGCCCGACTGGTCCATGCTGCTGGCCGCCATCACCACCG

TGTTCCTGGCCGCCGAGAAGCAGTGGATGATGCTGGACTGGAAGCCCAAGCGCCCCGACA

TGCTGGTGGACCCCTTCGGCCTGGGCTCCATCGTGCAGGACGGCCTGGTGTTCCGCCAGAA

CTTCTCCATCCGCTCCTACGAGATCGGCGCCGACCGCACCGCCTCCATCGAGACCGTGATG

AACCACCTGCAGGAGACCGCCCTGAACCACGTGAAGATCGCCGGCCTGTCCAACGACGGC

TTCGGCCGCACCCCCGAGATGTACAAGCGCGACCTGATCTGGGTGGTGGCCAAGATGCAG

GTGATGGTGAACCGCTACCCCACCTGGGGCGACACCGTGGAGGTGAACACCTGGGTGGCC

AAGTCCGGCAAGAACGGCATGCGCCGCGACTGGCTGATCTCCGACTGCAACACCGGCGAG

ATCCTGACCCGCGCCTCCTCCGTGTGGGTGATGATGAACCAGAAGACCCGCCGCCTGTCC

AAGATCCCCGACGAGGTGCGCAACGAGATCGAGCCCCACTTCGTGGACTCCGCCCCCGTG

GTGGAGGACGACGACCGCAAGCTGCCCAAGCTGGACGAGAACACCGCCGACTCCATCCG

CAAGGGCCTGACCCCCCGCTGGAACGACCTGGACGTGAACCAGCACGTGAACAACGTGA

AGTACATCGGCTGGATCCTGGAGTCCACCCCCCCCGAGGTGCTGGAGACCCAGGAGCTGT

GCTCCCTGACCCTGGAGTACCGCCGCGAGTGCGGCCGCGAGTCCGTGCTGGAGTCCCTGA

CCGCCGTGGACCCCTCCGCCGAGGGCTACGCCTCCCGCTTCCAGCACCTGCTGCGCCTGGA

GGACGGCGGCGAGATCGTGAAGGCCCGCACCGAGTGGCGCCCCAAGAACGCCGGCATCA

ACGGCGTGGTGCCCTCCGAGGAGTCCTCCCCCGGCGACTTCTTCTGA SEQ ID NO: 34

Cuphea wrightii (Cw) FATB4b amino acid sequence

MVATAASSAFFPVPSADTSSSRPGKLGNGPSSLSPLKPKSIPNGGLQVKANASAPPKINGSSVGL

KSGSFKTQEDAPSAPPPRTFINQLPDWSMLLAAITTVFLAAEKQWMMLDWKPKRPDMLVDPF

GLGSIVQDGLVFRQNFSIRSYEIGADRTASIETVMNHLQETALNHVKIAGLSSDGFGRTPAMSK

RDLIWWAKMQVMVNRYPAWGDTVEVNTWVAKSGKNGMRRDWLISDCNTGEILTRASSV

WVMMNQKTRRLSKIPDEVRNEIEPHFVDSAPWEDDDRKLPKLDENTADSIRKGLTPRWNDL

DVNQHVNNVKYIGWILESTPAEVLETQELCSLTLEYRRECGRESVLESLTAVDPSGEGDGSKF QHLLRLEDGGEIVKARTEWRPKNAGINGWPSEESSPGGDFF* SEQ ID NO: 35

Cuphea wrightii (Cw) FATB4b coding DNA sequence

TTGGTGGCTACCGCTGCAAGTTCTGCATTTTTCCCCGTACCATCCGCCGACACCTCCTCATC

GAGACCCGGAAAGCTCGGCAATGGGCCATCGAGCTTGAGCCCCCTCAAGCCGAAATCGAT

CCCCAATGGCGGGTTGCAGGTTAAGGCAAACGCCAGTGCCCCTCCTAAGATCAATGGTTC

CTCGGTCGGTCTGAAGTCGGGCAGTTTCAAGACTCAGGAAGACGCTCCTTCGGCCCCTCCT

CCTCGGACTTTTATCAACCAGTTGCCTGATTGGAGTATGCTTCTTGCTGCAATCACTACTGT

CTTCTTGGCTGCAGAGAAGCAGTGGATGATGCTTGATTGGAAACCTAAGAGGCCTGACAT

GCTTGTCGACCCGTT CGGATT GGGAAGT ATT GTT CAGGATGGGCTTGTTTT CAGGC AGAAT

TTCTCGATTAGGTCCTACGAAATAGGCGCTGATCGCACTGCGTCTATAGAGACGGTGATG

AACCATTTGCAGGAAACAGCTCTCAATCATGTTAAGATTGCTGGGCTTTCTAGTGATGGCT

TTGGTCGTACTCCTGCGATGTCTAAACGGGACCTCATTTGGGTTGTTGCGAAAATGCAGGT

CATGGTTAACCGCTACCCTGCTTGGGGTGACACGGTTGAAGTGAATACTTGGGTTGCCAA

GTCAGGGAAAAATGGTATGCGTCGTGACTGGCTCATAAGTGATTGCAACACTGGAGAGAT

TCTTACAAGAGCATCAAGCGTGTGGGTCATGATGAATCAAAAGACAAGAAGATTGTCAAA

AATTCCAGATGAGGTTCGAAATGAGATAGAGCCTCATTTTGTGGACTCTGCGCCCGTCGTT

GAAGACGATGACCGGAAACTTCCCAAGCTGGATGAGAACACTGCTGACTCCATCCGCAAG

GGTCTAACTCCGAGGTGGAATGACTTGGATGTCAATCAGCACGTCAACAACGTGAAGTAC

ATTGGGTGGATTCTTGAGAGTACTCCAGCAGAAGTTCTGGAGACCCAGGAATTATGTTCCC

TGACCCTGGAATACAGGCGGGAATGTGGAAGGGAGAGCGTGCTGGAGTCCCTCACTGCTG

TAGATCCGTCTGGAGAGGGCGATGGGTCCAAGTTCCAGCACCTTCTGCGGCTTGAGGATG

GAGGTGAGATCGTGAAGGCGAGAACTGAGTGGCGACCAAAGAATGCTGGAATCAATGGG

GTGGTACCATCCGAGGAGTCCTCACCTGGTGGAGACTTCTTTTAA SEQ ID NO: 36

Cuphea wrightii (Cw) FATB4b coding DNA sequence codon optimized for Prototheca moriformis

AT GGT GGCC ACCGCCGCCT CCT CCGCCTT CTTCCCCGTGCCCTCCGCCG AC ACCTCCT CCT C

CCGCCCCGGCAAGCTGGGCAACGGCCCCTCCTCCCTGTCCCCCCTGAAGCCCAAGTCCATC

CCCAACGGCGGCCTGCAGGTGAAGGCCAACGCCTCCGCCCCCCCCAAGATCAACGGCTCC

TCCGTGGGCCTGAAGTCCGGCTCCTTCAAGACCCAGGAGGACGCCCCCTCCGCCCCCCCCC

CCCGCACCTTCATCAACCAGCTGCCCGACTGGTCCATGCTGCTGGCCGCCATCACCACCGT

GTTCCTGGCCGCCGAGAAGCAGTGGATGATGCTGGACTGGAAGCCCAAGCGCCCCGACAT

GCTGGTGGACCCCTTCGGCCTGGGCTCCATCGTGCAGGACGGCCTGGTGTTCCGCCAGAA

CTTCTCCATCCGCTCCTACGAGATCGGCGCCGACCGCACCGCCTCCATCGAGACCGTGATG

AACCACCTGCAGGAGACCGCCCTGAACCACGTGAAGATCGCCGGCCTGTCCTCCGACGGC

TTCGGCCGCACCCCCGCCATGTCCAAGCGCGACCTGATCTGGGTGGTGGCCAAGATGCAG

GTGATGGTGAACCGCTACCCCGCCTGGGGCGACACCGTGGAGGTGAACACCTGGGTGGCC

AAGTCCGGCAAGAACGGCATGCGCCGCGACTGGCTGATCTCCGACTGCAACACCGGCGAG

ATCCTGACCCGCGCCTCCTCCGTGTGGGTGATGATGAACCAGAAGACCCGCCGCCTGTCC

AAGATCCCCGACGAGGTGCGCAACGAGATCGAGCCCCACTTCGTGGACTCCGCCCCCGTG

GTGGAGGACGACGACCGCAAGCTGCCCAAGCTGGACGAGAACACCGCCGACTCCATCCG

CAAGGGCCTGACCCCCCGCTGGAACGACCTGGACGTGAACCAGCACGTGAACAACGTGA

AGTACATCGGCTGGATCCTGGAGTCCACCCCCGCCGAGGTGCTGGAGACCCAGGAGCTGT

GCTCCCTGACCCTGGAGTACCGCCGCGAGTGCGGCCGCGAGTCCGTGCTGGAGTCCCTGA

CCGCCGTGGACCCCTCCGGCGAGGGCGACGGCTCCAAGTTCCAGCACCTGCTGCGCCTGG

AGGACGGCGGCGAGATCGTGAAGGCCCGCACCGAGTGGCGCCCCAAGAACGCCGGCATC

AACGGCGTGGTGCCCTCCGAGGAGTCCTCCCCCGGCGGCGACTTCTTCTGA SEQ ID NO: 37

Cuphea wrightii (Cw) FATB5 amino acid sequence

MVAAAASSAFFSVPTPGTPPKPGKFGNWPSSLSVPFKPDNGGFHVKANASAHPKANGSAVNL

KSGSLETPPRSFINQLPDLSVLLSKITTVFGAAEKQWKRPGMLVEPFGVDRIFQDGVFFRQSFSI

RSYEIGVDRTASIETLMNIFQETSLNHCKSIGLLNDGFGRTPEMCKRDLIWWTKIQVEVNRYP

TWGDTIEVNTWVSESGKNGMGRDWLISDCRTGEILIRATSVWAMMNQNTRRLSKFPYEVRQE

IAPHFVDSAPVIEDDQKLQKLDVKTGDSIRDGLTPRWNDLDVNQHVNNVKYIGWILKSVPIEV

FETQELCGVTLEYRRECGRDSVLESVTAMDPAKEGDRCVYQHLLRLEDGADITIGRTEWRPK NAGANGAMSSGKTSNGNCLIEGRGWQPFRWRLIF* SEQ ID NO: 38

Cuphea wrightii (Cw) FATB5 coding DNA sequence

ATGGTGGCTGCCGCAGCAAGTTCTGCATTCTTCTCTGTTCCAACCCCGGGAACGCCCCCTA

AACCCGGGAAGTTCGGTAACTGGCCATCGAGCTTGAGCGTCCCCTTCAAGCCCGACAATG

GTGGCTTTCATGTCAAGGCAAACGCCAGTGCCCATCCTAAGGCTAATGGTTCTGCGGTAA

ATCTAAAGTCTGGCAGCCTCGAGACTCCTCCTCGGAGTTTCATTAACCAGCTGCCGGACTT

GAGTGTGCTTCTGTCCAAAATCACGACTGTCTTCGGGGCGGCTGAGAAGCAGTGGAAGAG

GCCCGGCATGCTCGTGGAACCGTTTGGGGTTGACAGGATTTTTCAGGATGGTGTTTTTTTC

AGACAGAGTTTTTCTATCAGGTCTTACGAAATAGGCGTTGATCGAACAGCCTCGATAGAG

ACACTGATGAACATCTTCCAGGAAACATCTTTGAATCATTGCAAGAGTATCGGTCTTCTCA

ACGATGGCTTTGGTCGTACTCCTGAGATGTGTAAGAGGGACCTCATTTGGGTGGTTACGAA

AATTCAGGTCGAGGTGAATCGCTATCCTACTTGGGGTGACACTATCGAAGTCAATACTTGG

GTCTCGGAGTCGGGGAAAAACGGTATGGGTCGGGATTGGCTGATAAGTGATTGCCGTACT

GGAGAGATTCTTATAAGAGCAACGAGCGTGTGGGCGATGATGAATCAAAACACGAGAAG

ATTGTCAAAATTTCCATATGAGGTTCGACAGGAGATAGCGCCTCATTTTGTGGACTCTGCT

CCTGTCATTGAAGACGATCAAAAGTTGCAGAAGCTTGATGTGAAGACAGGTGATTCCATT

CGCGATGGTCTAACTCCGAGATGGAATGACTTGGATGTCAATCAACACGTTAACAATGTG

AAGTACATTGGATGGATTCTCAAGAGTGTTCCAATAGAAGTTTTCGAGACACAGGAGCTA

TGCGGCGTCACACTTGAATATAGGCGGGAATGCGGAAGGGACAGTGTGCTGGAGTCAGTG

ACCGCTATGGATCCAGCAAAAGAGGGAGACCGGTGTGTGTACCAGCACCTTCTTCGGCTT

GAGGATGGAGCTGATATCACTATAGGCAGAACCGAGTGGCGGCCGAAGAATGCAGGAGC

CAATGGTGCAATGTCATCAGGAAAGACTTCAAATGGAAACTGTCTCATAGAAGGAAGGGG

TTGGCAACCTTTCCGAGTTGTGCGTTTAATTTTCTGA SEQ ID NO: 39

Cuphea wrightii (Cw) FATB5 coding DNA sequence codon optimized for Prototheca moriformis

ATGGTGGCCGCCGCCGCCTCCTCCGCCTTCTTCTCCGTGCCCACCCCCGGCACCCCCCCCA

AGCCCGGCAAGTTCGGCAACTGGCCCTCCTCCCTGTCCGTGCCCTTCAAGCCCGACAACGG

CGGCTTCCACGTGAAGGCCAACGCCTCCGCCCACCCCAAGGCCAACGGCTCCGCCGTGAA

CCTGAAGTCCGGCTCCCTGGAGACCCCCCCCCGCTCCTTCATCAACCAGCTGCCCGACCTG

TCCGTGCTGCTGTCCAAGATCACCACCGTGTTCGGCGCCGCCGAGAAGCAGTGGAAGCGC

CCCGGCATGCTGGTGGAGCCCTTCGGCGTGGACCGCATCTTCCAGGACGGCGTGTTCTTCC

GCCAGTCCTTCTCCATCCGCTCCTACGAGATCGGCGTGGACCGCACCGCCTCCATCGAGAC

CCTGATGAACATCTTCCAGGAGACCTCCCTGAACCACTGCAAGTCCATCGGCCTGCTGAAC

GACGGCTTCGGCCGCACCCCCGAGATGTGCAAGCGCGACCTGATCTGGGTGGTGACCAAG

ATCCAGGTGGAGGTGAACCGCTACCCCACCTGGGGCGACACCATCGAGGTGAACACCTGG

GTGTCCGAGTCCGGCAAGAACGGCATGGGCCGCGACTGGCTGATCTCCGACTGCCGCACC

GGCGAGATCCTGATCCGCGCCACCTCCGTGTGGGCCATGATGAACCAGAACACCCGCCGC

CTGTCCAAGTTCCCCTACGAGGTGCGCCAGGAGATCGCCCCCCACTTCGTGGACTCCGCCC

CCGTGATCGAGGACGACCAGAAGCTGCAGAAGCTGGACGTGAAGACCGGCGACTCCATC

CGCGACGGCCTGACCCCCCGCTGGAACGACCTGGACGTGAACCAGCACGTGAACAACGTG

AAGTACATCGGCTGGATCCTGAAGTCCGTGCCCATCGAGGTGTTCGAGACCCAGGAGCTG

TGCGGCGTGACCCTGGAGTACCGCCGCGAGTGCGGCCGCGACTCCGTGCTGGAGTCCGTG

ACCGCCATGGACCCCGCCAAGGAGGGCGACCGCTGCGTGTACCAGCACCTGCTGCGCCTG

GAGGACGGCGCCGACATCACCATCGGCCGCACCGAGTGGCGCCCCAAGAACGCCGGCGC

CAACGGCGCCATGTCCTCCGGCAAGACCTCCAACGGCAACTGCCTGATCGAGGGCCGCGG

CTGGCAGCCCTTCCGCGTGGTGCGCCTGATCTTCTGA SEQ ID NO: 40

Cuphea heterophylla (Cht) FATBla amino acid sequence

MVAAAASSAFFSVPTPGTSTKPGNFGNWPSSLSVPFKPESNHNGGFRVKANASAHPKANGSA

VNLKSGSLETQEDTSSSSPPPRTFIKQLPDWGMLLSKITTVFGAAERQWKRPGMLVEPFGVDRI

FQDGVFFRQSFSIRSYEIGADRTASIETLMNIFQETSLNHCKSIGLLNDGFGRTPEMCKRDLIWV

VTKIQVEVNRYPTWGDTIEVNTWVSESGKNGMGRDWLISDCRTGEILIRATSVWAMMNRKT

RRLSKFPYEVRQEIAPHFVDSAPVIEDDKKLHKLDVKTGDSIRKGLTPRWNDLDVNQHVNNV

KYIGWILKSVPAEVFETQELCGVTLEYRRECGRDSVLESVTAMDTAKEGDRSLYQHLLRLEDG ADITIGRTEWRPKNAGANGAISTGKTSNENSVS* SEQ ID NO: 41

Cuphea heterophylla (Cht) FATBla coding DNA sequence

ATGGTGGCTGCCGCAGCAAGTTCTGCATTCTTCTCCGTTCCAACCCCGGGAACCTCCACTA

AACCCGGGAACTTCGGCAATTGGCCATCGAGCTTGAGCGTCCCCTTCAAGCCCGAATCAA

ACCACAATGGTGGCTTTCGGGTCAAGGCAAACGCCAGTGCTCATCCTAAGGCTAACGGTT

CTGCAGTAAATCTAAAGTCTGGCAGCCTCGAGACTCAGGAGGACACTTCATCGTCGTCCC

CTCCTCCTCGGACTTTTATTAAGCAGTTGCCCGACTGGGGTATGCTTCTGTCCAAAATCAC

GACTGTCTTCGGGGCGGCTGAGAGGCAGTGGAAGAGGCCCGGCATGCTTGTGGAACCGTT

TACGAAATAGGCGCTGATCGAACAGCCTCAATAGAGACGCTGATGAACATCTTCCAGGAA

ACATCTCTGAATCATTGTAAGAGTATCGGTCTTCTCAATGACGGCTTTGGTCGTACTCCTG

AGATGTGTAAGAGGGACCTCATTTGGGTGGTTACGAAAATTCAGGTCGAGGTGAATCGCT

ATCCTACTTGGGGTGATACTATTGAGGTCAATACTTGGGTCTCAGAGTCGGGGAAAAACG

GTATGGGTCGTGATTGGCTGATAAGCGATTGCCGTACCGGAGAAATTCTTATAAGAGCAA

CGAGCGTGTGGGCTATGATGAATCGAAAGACGAGAAGATTGTCAAAATTTCCATATGAGG

TTCGACAGGAGATAGCGCCTCATTTTGTGGACTCTGCTCCTGTCATTGAAGACGATAAAAA

ATTGCACAAGCTTGATGTTAAGACGGGTGATTCCATTCGCAAGGGTCTAACTCCAAGGTG

GAATGACTTGGATGTCAATCAGCACGTTAACAATGTGAAGTACATTGGGTGGATTCTCAA

GAGTGTTCCAGCAGAAGTTTTCGAGACCCAGGAGCTATGCGGAGTCACCCTTGAGTACAG

GCGGGAATGTGGAAGGGACAGTGTGCTGGAGTCCGTGACCGCTATGGATACCGCAAAAG

AGGGAGACCGGTCTCTGTACCAGCACCTTCTTCGGCTTGAGGATGGGGCTGATATCACCAT

AGGCAGAACCGAGTGGCGGCCGAAGAATGCAGGAGCCAATGGGGCAATATCAACAGGAA

AGACTTCAAATGAAAACTCTGTCTCTTAG SEQ ID NO: 42

Cuphea heterophylla (Cht) FATBla coding DNA sequence codon optimized for Prototheca moriformis

AT GGT GGCCGCCGCCGCCT CCT CCGCCTT CTT CT CCGTGCCC ACCCCCGGC ACCT CC ACC A

AGCCCGGCAACTTCGGCAACTGGCCCTCCTCCCTGTCCGTGCCCTTCAAGCCCGAGTCCAA

CCACAACGGCGGCTTCCGCGTGAAGGCCAACGCCTCCGCCCACCCCAAGGCCAACGGCTC

CGCCGTGAACCTGAAGTCCGGCTCCCTGGAGACCCAGGAGGACACCTCCTCCTCCTCCCCC

CCCCCCCGCACCTTCATCAAGCAGCTGCCCGACTGGGGCATGCTGCTGTCCAAGATCACCA

CCGTGTTCGGCGCCGCCGAGCGCCAGTGGAAGCGCCCCGGCATGCTGGTGGAGCCCTTCG

GCGTGGACCGCATCTTCCAGGACGGCGTGTTCTTCCGCCAGTCCTTCTCCATCCGCTCCTA

CGAGATCGGCGCCGACCGCACCGCCTCCATCGAGACCCTGATGAACATCTTCCAGGAGAC

CTCCCTGAACCACTGCAAGTCCATCGGCCTGCTGAACGACGGCTTCGGCCGCACCCCCGA

GATGTGCAAGCGCGACCTGATCTGGGTGGTGACCAAGATCCAGGTGGAGGTGAACCGCTA

CCCCACCTGGGGCGACACCATCGAGGTGAACACCTGGGTGTCCGAGTCCGGCAAGAACGG

CATGGGCCGCGACTGGCTGATCTCCGACTGCCGCACCGGCGAGATCCTGATCCGCGCCAC

CTCCGTGTGGGCCATGATGAACCGCAAGACCCGCCGCCTGTCCAAGTTCCCCTACGAGGT

GCGCCAGGAGATCGCCCCCCACTTCGTGGACTCCGCCCCCGTGATCGAGGACGACAAGAA

GCTGCACAAGCTGGACGTGAAGACCGGCGACTCCATCCGCAAGGGCCTGACCCCCCGCTG

GAACGACCTGGACGTGAACCAGCACGTGAACAACGTGAAGTACATCGGCTGGATCCTGAA

GTCCGTGCCCGCCGAGGTGTTCGAGACCCAGGAGCTGTGCGGCGTGACCCTGGAGTACCG

CCGCGAGTGCGGCCGCGACTCCGTGCTGGAGTCCGTGACCGCCATGGACACCGCCAAGGA

GGGCGACCGCTCCCTGTACCAGCACCTGCTGCGCCTGGAGGACGGCGCCGACATCACCAT

CGGCCGCACCGAGTGGCGCCCCAAGAACGCCGGCGCCAACGGCGCCATCTCCACCGGCAA

GACCTCCAACGAGAACTCCGTGTCCTGA SEQ ID NO: 43

Cuphea heterophylla (Cht) FATBlb (P16S, T20P, G94S, G105W, S293F, L305F variant) amino acid sequence

MVAAAAS S AFF S VPTSGT SPKPGNF GNWP S SLS VPFKPES SHNGGF QVKANAS AHPKANGS AV

NLKSGSLETQEDT S S S SPPPRTFIKQLPD W SMLLSKITTVF WAAERQ WKRPGMLVEPF GVDRIF

QDGVFFRQ SF SIRS YEIGADRT ASIETLMNIF QETSLNHCKSIGLLNDGF GRTPEMCKRDLIWW

TKIQVEVNRYPTWGDTIEVNTWVSESGKNGMGRDWLISDCRTGEILIRATSVWAMMNRKTRR

LSKFPYEVRQEIAPHFVDSAPVIEDDKKLHKLDVKTGDFIRKGLTPRWNDFDVNQHVNNVKYI

GWILKSVPAEVFETQELCGVTLEYRRECGRDSVLESVTAMDTAKEGDRSLYQHLLRLEDGADI TIGRTEWRPKNAGANGAISTGKTSNENSVS* SEQ ID NO: 44

Cuphea heterophylla (Cht) FATBlb(P16S, T20P, G94S, G105W, S293F, L305F variant) coding DNA sequence

ATGGTGGCTGCCGCAGCAAGTTCTGCATTCTTCTCCGTTCCAACCTCGGGAACCTCCCCTA

AACCCGGGAACTTCGGCAATTGGCCATCGAGCTTGAGCGTCCCCTTCAAGCCCGAATCAA

GCCACAATGGTGGCTTTCAGGTCAAGGCAAACGCCAGTGCCCATCCTAAGGCTAACGGTT

CTGCAGTAAATCTAAAGTCTGGCAGCCTCGAGACTCAGGAGGACACTTCATCGTCGTCCC

CTCCTCCTCGGACTTTTATTAAGCAGTTGCCCGACTGGAGTATGCTTCTGTCCAAAATCAC

GACTGTCTTCTGGGCGGCTGAGAGGCAGTGGAAGAGGCCCGGCATGCTTGTGGAACCGTT

TGGGGTTGACAGGATTTTTCAGGATGGGGTTTTTTTCAGACAGAGTTTTTCGATCAGGTCT

TACGAAATAGGCGCTGATCGAACAGCCTCAATAGAGACGCTGATGAACATCTTCCAGGAA

ACATCTCTGAATCATTGTAAGAGTATCGGTCTTCTCAATGACGGCTTTGGTCGTACTCCTG

AGATGTGTAAGAGGGACCTCATTTGGGTGGTTACGAAAATTCAGGTCGAGGTGAATCGCT

ATCCTACTTGGGGTGATACTATTGAGGTCAATACTTGGGTCTCAGAGTCGGGGAAAAACG

GTATGGGTCGTGATTGGCTGATAAGCGATTGCCGTACCGGAGAAATTCTTATAAGAGCAA

CGAGCGTGTGGGCTATGATGAATCGAAAGACGAGAAGATTGTCAAAATTTCCATATGAGG

TTCGACAGGAGATAGCGCCTCATTTTGTGGACTCTGCTCCTGTCATTGAAGACGATAAAAA

ATTGCACAAGCTTGATGTTAAGACGGGTGATTTCATTCGCAAGGGTCTAACTCCAAGGTG

GAATGACTTTGATGTCAATCAGCACGTTAACAATGTGAAGTACATTGGGTGGATTCTCAA

GAGTGTTCCAGCAGAAGTTTTCGAGACCCAGGAGCTATGCGGAGTCACCCTTGAGTATAG

GCGGGAATGTGGAAGGGACAGTGTGCTGGAGTCCGTGACCGCTATGGATACCGCAAAAG

AGGGAGACCGGTCTCTGTACCAGCACCTTCTTCGGCTTGAGGATGGGGCTGATATCACCAT

AGGCAGAACCGAGTGGCGGCCGAAGAATGCAGGAGCCAATGGGGCAATATCAACAGGAA

AGACTTCAAATGAAAACTCTGTCTCTTAG SEQ ID NO: 45

Cuphea heterophylla (Cht) FATBlb (P16S, T20P, G94S, G105W, S293F, L305F variant) coding DNA sequence codon optimized for Prototheca moriformis

ATGGTGGCCGCCGCCGCCTCCTCCGCCTTCTTCTCCGTGCCCACCTCCGGCACCTCCCCCA

AGCCCGGCAACTTCGGCAACTGGCCCTCCTCCCTGTCCGTGCCCTTCAAGCCCGAGTCCTC

CCACAACGGCGGCTTCCAGGTGAAGGCCAACGCCTCCGCCCACCCCAAGGCCAACGGCTC

CGCCGTGAACCTGAAGTCCGGCTCCCTGGAGACCCAGGAGGACACCTCCTCCTCCTCCCCC

CCCCCCCGCACCTTCATCAAGCAGCTGCCCGACTGGTCCATGCTGCTGTCCAAGATCACCA

CCGTGTTCTGGGCCGCCGAGCGCCAGTGGAAGCGCCCCGGCATGCTGGTGGAGCCCTTCG

GCGTGGACCGCATCTTCCAGGACGGCGTGTTCTTCCGCCAGTCCTTCTCCATCCGCTCCTA

CGAGATCGGCGCCGACCGCACCGCCTCCATCGAGACCCTGATGAACATCTTCCAGGAGAC

CTCCCTGAACCACTGCAAGTCCATCGGCCTGCTGAACGACGGCTTCGGCCGCACCCCCGA

GATGTGCAAGCGCGACCTGATCTGGGTGGTGACCAAGATCCAGGTGGAGGTGAACCGCTA

CCCCACCTGGGGCGACACCATCGAGGTGAACACCTGGGTGTCCGAGTCCGGCAAGAACGG

CATGGGCCGCGACTGGCTGATCTCCGACTGCCGCACCGGCGAGATCCTGATCCGCGCCAC

CTCCGTGTGGGCCATGATGAACCGCAAGACCCGCCGCCTGTCCAAGTTCCCCTACGAGGT

GCGCCAGGAGATCGCCCCCCACTTCGTGGACTCCGCCCCCGTGATCGAGGACGACAAGAA

GCTGCACAAGCTGGACGTGAAGACCGGCGACTTCATCCGCAAGGGCCTGACCCCCCGCTG

GAACGACTTCGACGTGAACCAGCACGTGAACAACGTGAAGTACATCGGCTGGATCCTGAA

GTCCGTGCCCGCCGAGGTGTTCGAGACCCAGGAGCTGTGCGGCGTGACCCTGGAGTACCG

CCGCGAGTGCGGCCGCGACTCCGTGCTGGAGTCCGTGACCGCCATGGACACCGCCAAGGA

GGGCGACCGCTCCCTGTACCAGCACCTGCTGCGCCTGGAGGACGGCGCCGACATCACCAT

CGGCCGCACCGAGTGGCGCCCCAAGAACGCCGGCGCCAACGGCGCCATCTCCACCGGCAA

GACCTCCAACGAGAACTCCGTGTCCTGA SEQ ID NO: 46

Cuphea heterophylla (Cht) FATB2b amino acid sequence

MWAAAASSAFFPVPASGTSPKPGKFGTWLSSSSPSYKPKSNPSGGFQVKANASAHPKANGSA

VSLKSGSLNTQEGTSSSPPPRTFLNQLPDWSRLRTAITTVFVAAEKQLTMLDRKSKKPDMHVD

WFGLEIIVQDGLVFRESFSIRSYEIGADRTASIETLMNHLQDTSLNHCKSVGLLNDGFGRTPEM

CKRDLIWVLTKMQIMVNRYPTWGDTVEINSWFSQSGKIGMGRNWLISDCNTGEILIRATSIWA

MMNQKTRRFSKLPNEVRQEIAPHFVDAPPVIEDNDRKLHKFDVKTGDSICKGLTPEWNDLDV

NQHVSNVKYIGWILESMPKEVLDTQELCSLTLEYRRECGRDSVLESVTAMDPSKVGDRSQYQ HLLRLEDGTDIMKGRTEWRPKNAGTNGAISTGKTSNGNSVS* SEQ ID NO: 47

Cuphea heterophylla (Cht) FATB2b coding DNA sequence

ATGGTGGTGGCTGCTGCAGCAAGCTCTGCATTCTTCCCTGTTCCGGCATCTGGAACCTCCC

CTAAACCCGGGAAGTTCGGGACTTGGCTATCGAGCTCGAGCCCTTCCTACAAGCCCAAGT

CAAACCCCAGTGGTGGATTTCAGGTTAAGGCAAATGCCAGTGCTCATCCTAAGGCTAACG

GTTCCGCAGTAAGTCTAAAGTCTGGCAGCCTCAACACTCAGGAGGGCACTTCGTCGTCCCC

TCCTCCTCGGACTTTCCTTAACCAGTTGCCTGATTGGAGTAGGCTTCGGACTGCAATCACG

ACCGTCTTCGTGGCGGCAGAGAAGCAGTTGACTATGCTCGATCGAAAGTCTAAGAAGCCT

GACATGCACGTGGACTGGTTTGGGTTGGAGATTATTGTTCAGGATGGGCTCGTGTTCAGAG

AGAGTTTTTCGATCAGGTCTTACGAAATAGGCGCTGATCGAACAGCCTCTATAGAAACGTT

GATGAACCATTTGCAGGACACATCTTTGAACCATTGTAAGAGTGTGGGTCTTCTCAATGAC

GGCTTTGGTCGTACCCCGGAGATGTGTAAAAGGGACCTCATTTGGGTGCTTACAAAAATG

CAGATCATGGTGAATCGCTATCCAACTTGGGGCGATACTGTCGAGATCAATAGCTGGTTCT

CCCAGTCCGGGAAAATCGGTATGGGTCGCAATTGGCTAATAAGTGATTGCAACACAGGAG

AAATTCTTATAAGAGCAACGAGCATTTGGGCCATGATGAATCAAAAGACGAGAAGATTCT

CAAAACTTCCAAACGAGGTTCGCCAGGAGATAGCGCCTCATTTTGTGGACGCCCCTCCTGT

CATTGAAGACAATGATCGAAAATTGCATAAGTTTGATGTGAAGACTGGTGATTCCATTTGC

AAGGGTCTAACACCGGAGTGGAATGACTTGGATGTCAATCAGCACGTAAGCAACGTGAAG

TACATTGGGT GGATTCTCGAGAGTATGCC AAAAGAAGTTTT GGAC ACCC AGGAGCT AT GC

TCTCTCACCCTTGAATATAGGCGGGAATGCGGAAGGGATAGTGTGCTGGAGTCTGTGACC

GCTATGGATCCCTCAAAAGTTGGAGACCGATCTCAGTACCAGCACCTTCTGCGGCTTGAA

GATGGGACTGATATCATGAAGGGCAGAACTGAGTGGCGACCAAAGAATGCAGGAACCAA

CGGGGCTATATCAACAGGAAAGACTTCAAATGGAAACTCGGTCTCTTAG SEQ ID NO: 48

Cuphea heterophylla (Cht) FATB2b coding DNA sequence codon optimized for Prototheca moriformis

ATGGTGGTGGCCGCCGCCGCCTCCTCCGCCTTCTTCCCCGTGCCCGCCTCCGGCACCTCCC

CCAAGCCCGGCAAGTTCGGCACCTGGCTGTCCTCCTCCTCCCCCTCCTACAAGCCCAAGTC

CAACCCCTCCGGCGGCTTCCAGGTGAAGGCCAACGCCTCCGCCCACCCCAAGGCCAACGG

CTCCGCCGTGTCCCTGAAGTCCGGCTCCCTGAACACCCAGGAGGGCACCTCCTCCTCCCCC

CCCCCCCGCACCTTCCTGAACCAGCTGCCCGACTGGTCCCGCCTGCGCACCGCCATCACCA

CCGTGTTCGTGGCCGCCGAGAAGCAGCTGACCATGCTGGACCGCAAGTCCAAGAAGCCCG

ACATGCACGTGGACTGGTTCGGCCTGGAGATCATCGTGCAGGACGGCCTGGTGTTCCGCG

AGTCCTTCTCCATCCGCTCCTACGAGATCGGCGCCGACCGCACCGCCTCCATCGAGACCCT

GATGAACCACCTGCAGGACACCTCCCTGAACCACTGCAAGTCCGTGGGCCTGCTGAACGA

CGGCTTCGGCCGCACCCCCGAGATGTGCAAGCGCGACCTGATCTGGGTGCTGACCAAGAT

GCAGATCATGGTGAACCGCTACCCCACCTGGGGCGACACCGTGGAGATCAACTCCTGGTT

CTCCCAGTCCGGCAAGATCGGCATGGGCCGCAACTGGCTGATCTCCGACTGCAACACCGG

CGAGATCCTGATCCGCGCCACCTCCATCTGGGCCATGATGAACCAGAAGACCCGCCGCTT

CTCCAAGCTGCCCAACGAGGTGCGCCAGGAGATCGCCCCCCACTTCGTGGACGCCCCCCC

CGTGATCGAGGACAACGACCGCAAGCTGCACAAGTTCGACGTGAAGACCGGCGACTCCAT

CTGCAAGGGCCTGACCCCCGAGTGGAACGACCTGGACGTGAACCAGCACGTGTCCAACGT

GAAGTACATCGGCTGGATCCTGGAGTCCATGCCCAAGGAGGTGCTGGACACCCAGGAGCT

GTGCTCCCTGACCCTGGAGTACCGCCGCGAGTGCGGCCGCGACTCCGTGCTGGAGTCCGT

GACCGCCATGGACCCCTCCAAGGTGGGCGACCGCTCCCAGTACCAGCACCTGCTGCGCCT

GGAGGACGGCACCGACATCATGAAGGGCCGCACCGAGTGGCGCCCCAAGAACGCCGGCA

CCAACGGCGCCATCTCCACCGGCAAGACCTCCAACGGCAACTCCGTGTCCTGA SEQ ID NO: 49

Cuphea heterophylla (Cht) FATB2a (S17P, P21S, T28N, L30P, S33L, G76D, S78P, G137W variant) amino acid sequence

MWAAAASSAFFPVPAPGTTSKPGKFGNWPSSLSPSFKPKSNPNGGFQVKANASAHPKANGS

AVSLKSGSLNTKEDTPSSPPPRTFLNQLPDWSRLRTAITTVFVAAEKQLTMLDRKSKKPDMHV

DWFGLEIIVQDWLVFRESFSIRSYEIGADRTASIETLMNHLQDTSLNHCKSVGLLNDGFGRTPE

MCKRDLIWVLTKMQIMVNRYPTWGDTVEINSWFSQSGKIGMGRNWLISDCNTGEILIRATSIW

AMMNQKTRRFSKLPNEVRQEIAPHFVDAPPLIEDNDRKLHKFDVKTGDSICKGLTPEWNDLD

VNQHVSNVKYIGWILESMPKEVLDTQELCSLTLEYRRECGRDSVLESVTAMDPSKVGDRSQY QHLLRLEDGTDIMKGRTEWRPKNAGTNGAISTGKTSNGNSVS* SEQ ID NO: 50

Cuphea heterophylla (Cht) FATB2a (S17P, P21S, T28N, L30P, S33L, G76D, S78P, G137W variant) coding DNA sequence

ATGGTGGTGGCTGCTGCAGCAAGTTCTGCATTCTTCCCTGTTCCAGCACCTGGAACCACGT

CTAAACCCGGGAAGTTCGGCAATTGGCCATCGAGCTTGAGCCCTTCCTTCAAGCCCAAGTC

AAACCCCAATGGTGGATTTCAGGTTAAGGCAAATGCCAGCGCTCATCCTAAGGCTAACGG

GTCTGCAGTAAGTCTAAAGTCTGGCAGCCTCAACACTAAGGAGGACACTCCGTCGTCCCC

TCCTCCTCGGACTTTCCTTAACCAGTTGCCTGATTGGAGTAGGCTTCGGACTGCAATCACG

ACCGTCTTCGTGGCGGCAGAGAAGCAGTTGACTATGCTCGATCGAAAGTCTAAGAAGCCT

GACATGCACGTGGACTGGTTTGGGTTGGAGATTATTGTTCAGGATTGGCTCGTGTTCAGAG

AGAGTTTTTCGATCAGGTCTTACGAAATAGGCGCTGATCGAACAGCCTCTATAGAAACGTT

GATGAACCATTTGCAGGACACATCTTTGAACCATTGTAAGAGTGTGGGTCTTCTCAATGAC

GGCTTTGGTCGTACCCCGGAGATGTGTAAAAGGGACCTCATTTGGGTGCTTACAAAAATG

CAGATCATGGTGAATCGCTATCCAACTTGGGGCGATACTGTCGAGATCAATAGCTGGTTCT

CCCAGTCCGGGAAAATCGGTATGGGTCGCAATTGGCTAATAAGTGATTGCAACACAGGAG

AAATTCTTATAAGAGCAACGAGCATTTGGGCCATGATGAATCAAAAGACGAGAAGATTCT

CAAAACTTCCAAACGAGGTTCGCCAGGAGATAGCTCCTCATTTTGTGGACGCCCCTCCTCT

CATTGAAGACAATGATCGAAAATTGCATAAGTTTGATGTGAAGACTGGTGATTCCATTTGC

AAGGGTCTAACACCGGAGTGGAATGACTTGGATGTCAATCAGCACGTAAGCAACGTGAAG

TACATTGGGTGGATTCTCGAGAGTATGCCAAAAGAAGTTTTGGACACCCAGGAGCTATGC

TCTCTCACCCTTGAATATAGGCGGGAATGCGGAAGGGACAGTGTGCTGGAGTCTGTGACC

GCTATGGATCCCTCAAAAGTTGGAGACCGATCTCAGTACCAGCACCTTCTGCGGCTTGAA

GATGGGACTGATATCATGAAGGGCAGAACTGAGTGGCGACCAAAGAATGCAGGAACCAA

CGGGGCGATATCAACAGGAAAGACTTCAAATGGAAACTCGGTCTCTTAG SEQ ID NO: 51

Cuphea heterophylla (Cht) FATB2a (S17P, P21S, T28N, L30P, S33L, G76D, S78P, G137W variant) coding DNA sequence codon optimized for Prototheca moriformis

ATGGTGGTGGCCGCCGCCGCCTCCTCCGCCTTCTTCCCCGTGCCCGCCCCCGGCACCACCT

CCAAGCCCGGCAAGTTCGGCAACTGGCCCTCCTCCCTGTCCCCCTCCTTCAAGCCCAAGTC

CAACCCCAACGGCGGCTTCCAGGTGAAGGCCAACGCCTCCGCCCACCCCAAGGCCAACGG

CTCCGCCGTGTCCCTGAAGTCCGGCTCCCTGAACACCAAGGAGGACACCCCCTCCTCCCCC

CCCCCCCGCACCTTCCTGAACCAGCTGCCCGACTGGTCCCGCCTGCGCACCGCCATCACCA

CCGTGTTCGTGGCCGCCGAGAAGCAGCTGACCATGCTGGACCGCAAGTCCAAGAAGCCCG

ACATGCACGTGGACTGGTTCGGCCTGGAGATCATCGTGCAGGACTGGCTGGTGTTCCGCG

AGTCCTTCTCCATCCGCTCCTACGAGATCGGCGCCGACCGCACCGCCTCCATCGAGACCCT

GAT GAACCACCT GCAGGACACCT CCCT GAACC ACTGC AAGT CCGT GGGCCTGCT GAACGA

CGGCTTCGGCCGCACCCCCGAGATGTGCAAGCGCGACCTGATCTGGGTGCTGACCAAGAT

GCAGATCATGGTGAACCGCTACCCCACCTGGGGCGACACCGTGGAGATCAACTCCTGGTT

CTCCCAGTCCGGCAAGATCGGCATGGGCCGCAACTGGCTGATCTCCGACTGCAACACCGG

CGAGATCCTGATCCGCGCCACCTCCATCTGGGCCATGATGAACCAGAAGACCCGCCGCTT

CTCCAAGCTGCCCAACGAGGTGCGCCAGGAGATCGCCCCCCACTTCGTGGACGCCCCCCC

CCTGATCGAGGACAACGACCGCAAGCTGCACAAGTTCGACGTGAAGACCGGCGACTCCAT

CTGC AAGGGCCT GACCCCCGAGT GGAACGACCT GGACGT GAACC AGCACGT GT CC AACGT

GAAGTACATCGGCTGGATCCTGGAGTCCATGCCCAAGGAGGTGCTGGACACCCAGGAGCT

GT GCT CCCT GACCCT GGAGT ACCGCCGCGAGT GCGGCCGCGACT CCGT GCT GGAGT CCGT

GACCGCCATGGACCCCTCCAAGGTGGGCGACCGCTCCCAGTACCAGCACCTGCTGCGCCT

GGAGGACGGCACCGACATCATGAAGGGCCGCACCGAGTGGCGCCCCAAGAACGCCGGCA

CCAACGGCGCCATCTCCACCGGCAAGACCTCCAACGGCAACTCCGTGTCCTGA SEQ ID NO: 52

Cuphea heterophylla (Cht) FATB2c (G76D, S78P variant) amino acid sequence

MWAAAASSAFFPVPASGTSPKPGKFGTWLSSSSPSYKPKSNPSGGFQVKANASAHPKANGSA

VSLKSGSLNTKEDTPSSPPPRTFLNQLPDWNRLRTAITTVFVAAEKQLTMLDRKSKKPDMHVD

WFGLEIIVQDGLVFRESFSIRSYEIGADRTASIETLMNHLQDTSLNHCKSVGLLNDGFGRTPEM

CKRDLIWVLTKMQIMVNRYPTWGDTVEINSWFSQSGKIGMGRNWLISDCNTGEILIRATSIWA

MMNQKTRRFSKLPNEWQEIAPHFVDAPPVIEDNDRKLHKFDVKTGDSICKGLTPEWNDLDV

NQHVSNVKYIGWILESMPKEVLDTQELCSLTLEYRRECGRDSVLESVTAMDPSKVGDRSQYQ HLLRLEDGTDIMKGRTEWRPKNAGTNGAISTGKTSNGNSVS* SEQ ID NO: 53

Cuphea heterophylla (Cht) FATB2c (G76D, S78P variant) coding DNA sequence

ATGGTGGTGGCTGCTGCAGCAAGCTCTGCATTCTTCCCTGTTCCGGCATCTGGAACCTCCC

CTAAACCCGGGAAGTTCGGGACTTGGCTATCGAGCTCGAGCCCTTCCTACAAGCCCAAGT

CAAACCCCAGTGGTGGATTTCAGGTTAAGGCAAATGCCAGTGCTCATCCTAAGGCTAACG

GTTCCGCAGTAAGTCTAAAGTCTGGCAGCCTCAACACTAAGGAGGACACTCCGTCGTCCC

CTCCTCCTCGGACTTTCCTTAACCAGTTGCCTGATTGGAATAGGCTTCGGACTGCAATCAC

GACCGTCTTCGTGGCGGCAGAGAAGCAGTTGACTATGCTCGATCGAAAGTCTAAGAAGCC

TGACATGCACGTGGACTGGTTTGGGTTGGAGATTATTGTTCAGGATGGGCTCGTGTTCAGA

GAGAGTTTTTCGATCAGGTCTTACGAAATAGGCGCTGATCGAACAGCCTCTATAGAAACG

TTGATGAACCATTTGCAGGACACATCTTTGAACCATTGTAAGAGTGTGGGTCTTCTCAATG

ACGGCTTTGGTCGTACCCCGGAGATGTGTAAAAGGGACCTCATTTGGGTGCTTACAAAAA

TGCAGATCATGGTGAATCGCTATCCAACTTGGGGCGATACTGTCGAGATCAATAGCTGGTT

CTCCCAGTCCGGGAAAATCGGTATGGGTCGCAATTGGCTAATAAGTGATTGCAACACAGG

AGAAATTCTTATAAGAGCAACGAGCATTTGGGCCATGATGAATCAAAAGACGAGAAGATT

CTCAAAACTTCCAAACGAGGTTCGCCAGGAGATAGCGCCTCATTTTGTGGACGCCCCTCCT

GTCATTGAAGACAATGATCGAAAATTGCATAAGTTTGATGTGAAGACTGGTGATTCCATTT

GCAAGGGTCTAACACCGGAGTGGAATGACTTGGATGTCAATCAGCACGTAAGCAACGTGA

AGTACATTGGGTGGATTCTCGAGAGTATGCCAAAAGAAGTTTTGGACACCCAGGAGCTAT

GCTCTCTCACCCTTGAATATAGGCGGGAATGCGGAAGGGACAGTGTGCTGGAGTCTGTGA

CCGCTATGGATCCCTCAAAAGTTGGGGACCGATCTCAGTACCAGCACCTTCTGCGGCTTGA

AGATGGGACTGATATCATGAAGGGCAGAACTGAGTGGCGACCAAAGAATGCAGGAACCA

ACGGGGCT AT AT CAACAGGAAAGACTT CAAATGGAAACTCGGTCTCTT AG SEQ ID NO: 54

Cuphea heterophylla (Cht) FATB2c (G76D, S78P variant) coding DNA sequence codon optimized for Prototheca moriformis

ATGGTGGTGGCCGCCGCCGCCTCCTCCGCCTTCTTCCCCGTGCCCGCCTCCGGCACCTCCC

CCAAGCCCGGCAAGTTCGGCACCTGGCTGTCCTCCTCCTCCCCCTCCTACAAGCCCAAGTC

CAACCCCTCCGGCGGCTTCCAGGTGAAGGCCAACGCCTCCGCCCACCCCAAGGCCAACGG

CTCCGCCGTGTCCCTGAAGTCCGGCTCCCTGAACACCAAGGAGGACACCCCCTCCTCCCCC

CCCCCCCGCACCTTCCTGAACCAGCTGCCCGACTGGAACCGCCTGCGCACCGCCATCACCA

CCGTGTTCGTGGCCGCCGAGAAGCAGCTGACCATGCTGGACCGCAAGTCCAAGAAGCCCG

ACATGCACGTGGACTGGTTCGGCCTGGAGATCATCGTGCAGGACGGCCTGGTGTTCCGCG

AGTCCTTCTCCATCCGCTCCTACGAGATCGGCGCCGACCGCACCGCCTCCATCGAGACCCT

GATGAACCACCTGCAGGACACCTCCCTGAACCACTGCAAGTCCGTGGGCCTGCTGAACGA

CGGCTTCGGCCGCACCCCCGAGATGTGCAAGCGCGACCTGATCTGGGTGCTGACCAAGAT

GCAGATCATGGTGAACCGCTACCCCACCTGGGGCGACACCGTGGAGATCAACTCCTGGTT

CTCCCAGTCCGGCAAGATCGGCATGGGCCGCAACTGGCTGATCTCCGACTGCAACACCGG

CGAGATCCTGATCCGCGCCACCTCCATCTGGGCCATGATGAACCAGAAGACCCGCCGCTT

CTCCAAGCTGCCCAACGAGGTGCGCCAGGAGATCGCCCCCCACTTCGTGGACGCCCCCCC

CGTGATCGAGGACAACGACCGCAAGCTGCACAAGTTCGACGTGAAGACCGGCGACTCCAT

CTGCAAGGGCCTGACCCCCGAGTGGAACGACCTGGACGTGAACCAGCACGTGTCCAACGT

GAAGTACATCGGCTGGATCCTGGAGTCCATGCCCAAGGAGGTGCTGGACACCCAGGAGCT

GTGCTCCCTGACCCTGGAGTACCGCCGCGAGTGCGGCCGCGACTCCGTGCTGGAGTCCGT

GACCGCCATGGACCCCTCCAAGGTGGGCGACCGCTCCCAGTACCAGCACCTGCTGCGCCT

GGAGGACGGCACCGACATCATGAAGGGCCGCACCGAGTGGCGCCCCAAGAACGCCGGCA

CCAACGGCGCCATCTCCACCGGCAAGACCTCCAACGGCAACTCCGTGTCCTGA SEQ ID NO: 55

Cuphea heterophylla (Cht) FATB2d (S21P, T28N, L30P, S33L, G76D, R97L, H124L, W127L, I132S, K258N, C303R, E309G, K334T, T386A variant) amino acid sequence

MWAAAASSAFFPVPAPGTTSKPGKFGNWPSSLSPSFKPKSNPNGGFQVKANASAHPKANGS

AVSLKSGSLNTQEDTSSSPPPRTFLNQLPDWSRLLTAISTVFVAAEKQLTMLDRKSKRPDMLV

DLFGLESIVQDGLVFRESYSIRSYEIGADRTASIETLMNHLQDTSLNHCKSVGLLNDGFGRTPE

MCKRDLIWVLTKMQIMVNRYPTWGDTVEINSWFSQSGKIGMGRNWLISDCNTGEILIRATSIW

AMMNQNTRRFSKLPNEVRQEIAPHFVDAPPVIEDNDRKLHKFDVKTGDSIRKGLTPGWNDLD

VNQHVSNVKYIGWILESMPTEVLETQELCSLTLEYRRECGRESVLESVTAMNPSKVGDRSQYQ HLLRLEDGADIMKGRTEWRPKNAGTNGAISTGKTSNGNSVS* SEQ ID NO: 56

Cuphea heterophylla (Cht) FATB2d (S21P, T28N, L30P, S33L, G76D, R97L, H124L, W127L, I132S, K258N, C303R, E309G, K334T, T386A variant) coding DNA sequence

ATGGTGGTGGCTGCTGCAGCAAGTTCTGCATTCTTCCCTGTTCCAGCACCTGGAACCACGT

CTAAACCCGGGAAGTTCGGCAATTGGCCATCGAGCTTGAGCCCTTCCTTCAAGCCCAAGTC

AAACCCCAATGGTGGATTTCAGGTTAAGGCAAATGCCAGTGCTCATCCTAAGGCTAACGG

TTCTGCGGTAAGTCTAAAGTCTGGCAGCCTCAACACTCAGGAGGACACTTCGTCGTCCCCT

CCTCCTCGGACATTCCTTAACCAGTTGCCTGATTGGAGTAGGCTTCTGACTGCAATCTCGA

CCGTCTTCGTGGCGGCAGAGAAGCAGTTGACTATGCTCGATCGAAAATCTAAGAGGCCTG

ACATGCTCGTGGACTTGTTTGGGTTGGAGAGTATTGTTCAGGATGGGCTCGTGTTCAGAGA

GAGTTATTCGATCAGGTCTTACGAAATAGGCGCTGATCGAACAGCCTCTATAGAAACGTT

GATGAACCATTTGCAGGACACATCTTTGAACCATTGTAAGAGTGTGGGTCTTCTCAATGAC

GGCTTTGGTCGTACCCCGGAGATGTGTAAAAGGGACCTCATTTGGGTGCTTACAAAAATG

CAGATCATGGTGAATCGCTATCCAACTTGGGGCGATACTGTCGAGATCAATAGCTGGTTCT

CCCAGTCCGGGAAAATCGGTATGGGTCGCAATTGGCTAATAAGTGATTGCAACACAGGAG

AAATTCTTATAAGAGCAACGAGCATTTGGGCCATGATGAATCAAAATACGAGAAGATTCT

CAAAACTTCCAAACGAGGTTCGCCAGGAGATAGCGCCTCATTTTGTTGACGCTCCTCCTGT

CATTGAAGACAATGATCGAAAATTGCATAAGTTTGATGTGAAGACTGGTGATTCCATTCG

CAAGGGTCTAACTCCGGGGTGGAATGACTTGGATGTCAATCAGCACGTAAGCAACGTGAA

GTACATTGGGTGGATTCTCGAGAGTATGCCAACAGAAGTTTTGGAGACCCAGGAGCTATG

CTCTCTCACCCTTGAATATAGGCGGGAATGCGGAAGGGAAAGTGTGCTGGAGTCCGTGAC

CGCTATGAATCCCTCAAAAGTTGGAGACCGGTCTCAGTACCAGCACCTTCTACGGCTTGAG

GATGGGGCTGATATCATGAAGGGCAGAACTGAGTGGCGACCAAAGAATGCAGGAACCAA

CGGGGCGATATCAACAGGAAAGACTTCAAATGGAAACTCGGTCTCTTAG SEQ ID NO: 57

Cuphea heterophylla (Cht) FATB2d (S21P, T28N, L30P, S33L, G76D, R97L, H124L, W127L, I132S, K258N, C303R, E309G, K334T, T386A variant) coding DNA sequence codon optimized for Prototheca moriformis

AT GGTGGT GGCCGCCGCCGCCT CCTCCGCCTTCTT CCCCGT GCCCGCCCCCGGC ACC ACCT

CCAAGCCCGGCAAGTTCGGCAACTGGCCCTCCTCCCTGTCCCCCTCCTTCAAGCCCAAGTC

CAACCCCAACGGCGGCTTCCAGGTGAAGGCCAACGCCTCCGCCCACCCCAAGGCCAACGG

CTCCGCCGTGTCCCTGAAGTCCGGCTCCCTGAACACCCAGGAGGACACCTCCTCCTCCCCC

CCCCCCCGCACCTTCCTGAACCAGCTGCCCGACTGGTCCCGCCTGCTGACCGCCATCTCCA

CCGTGTTCGTGGCCGCCGAGAAGCAGCTGACCATGCTGGACCGCAAGTCCAAGCGCCCCG

ACATGCTGGTGGACCTGTTCGGCCTGGAGTCCATCGTGCAGGACGGCCTGGTGTTCCGCG

AGTCCTACTCCATCCGCTCCTACGAGATCGGCGCCGACCGCACCGCCTCCATCGAGACCCT

GATGAACCACCTGCAGGACACCTCCCTGAACCACTGCAAGTCCGTGGGCCTGCTGAACGA

CGGCTTCGGCCGCACCCCCGAGATGTGCAAGCGCGACCTGATCTGGGTGCTGACCAAGAT

GCAGATCATGGTGAACCGCTACCCCACCTGGGGCGACACCGTGGAGATCAACTCCTGGTT

CTCCCAGTCCGGCAAGATCGGCATGGGCCGCAACTGGCTGATCTCCGACTGCAACACCGG

CGAGATCCTGATCCGCGCCACCTCCATCTGGGCCATGATGAACCAGAACACCCGCCGCTT

CTCCAAGCTGCCCAACGAGGT GCGCC AGGAGATCGCCCCCC ACTTCGT GGACGCCCCCCC

CGTGATCGAGGACAACGACCGCAAGCTGCACAAGTTCGACGTGAAGACCGGCGACTCCAT

CCGCAAGGGCCTGACCCCCGGCTGGAACGACCTGGACGTGAACCAGCACGTGTCCAACGT

GAAGTACATCGGCTGGATCCTGGAGTCCATGCCCACCGAGGTGCTGGAGACCCAGGAGCT

GTGCTCCCTGACCCTGGAGTACCGCCGCGAGTGCGGCCGCGAGTCCGTGCTGGAGTCCGT

GACCGCCATGAACCCCTCCAAGGTGGGCGACCGCTCCCAGTACCAGCACCTGCTGCGCCT

GGAGGACGGCGCCGACATCATGAAGGGCCGCACCGAGTGGCGCCCCAAGAACGCCGGCA

CCAACGGCGCCATCTCCACCGGCAAGACCTCCAACGGCAACTCCGTGTCCTGA SEQ ID NO: 58

Cuphea heterophylla (Cht) FATB2e (G76D, R97L, H124L, I132S, G152S, H165L, T211N, K258N, C303R, E309G, K334T, T386A variant) amino acid sequence

MWAAAASSAFFPVPASGTSPKPGKFGTWLSSSSPSYKPKSNPSGGFQVKANASAHPKANGSA

VSLKSGSLNTQEDTSSSPPPQTFLNQLPDWSRLLTAISTVFVAAEKQLTMLDRKSKRPDMLVD

WFGLESIVQDGLVFRESYSIRSYEISADRTASIETVMNLLQETSLNHCKSMGILNDGFGRTPEM

CKRDLIWVLTKMQILVNRYPNWGDTVEINSWFSQSGKIGMGRNWLISDCNTGEILIRATSIWA

MMNQNTRRFSKLPNEVRQEIAPHFVDAPPVIEDNDRKLHKFDVKTGDSIRKGLTPGWNDLDV

NQHVSNVKYIGWILESMPTEVLETQELCSLTLEYRRECGRDSVLESVTAMNPSKVGDRSQYQ HLLRLEDGADIMKGRTEWRPKNAGTNGAISTGKTSNGNSVS* SEQ ID NO: 59

Cuphea heterophylla (Cht) FATB2e (G76D, R97L, H124L, I132S, G152S, H165L, T211N, K258N, C303R, E309G, K334T, T386A variant) coding DNA sequence

ATGGTGGTGGCTGCTGCAGCAAGCTCTGCATTCTTCCCTGTTCCGGCATCTGGAACCTCCC

CTAAACCCGGGAAGTTCGGGACTTGGCTATCGAGCTCGAGCCCTTCCTACAAGCCCAAGT

CAAACCCCAGTGGTGGATTTCAGGTTAAGGCAAATGCCAGTGCTCATCCTAAGGCTAACG

GTTCTGCAGTAAGTCTAAAGTCTGGCAGCCTCAACACTCAGGAGGACACTTCGTCGTCCCC

TCCTCCTCAGACATTCCTTAACCAGTTGCCTGATTGGAGTAGGCTTCTGACAGCAATCTCG

ACCGTCTTCGTGGCGGCAGAGAAGCAGTTGACTATGCTCGATCGAAAATCTAAAAGGCCT

GACATGCTCGTGGACTGGTTTGGGTTGGAGAGTATTGTTCAGGATGGGCTCGTGTTCAGAG

AGAGTTATTCGATCAGGTCTTACGAAATAAGCGCTGATCGAACAGCCTCTATAGAGACGG

TGATGAACCTCTTGCAGGAAACATCTCTCAATCATTGTAAGAGTATGGGTATTCTCAATGA

CGGCTTTGGTCGTACCCCGGAGATGTGCAAAAGGGACCTCATTTGGGTGCTTACAAAAAT

GCAGATCTTGGTGAATCGCTATCCAAATTGGGGTGATACTGTCGAGATCAATAGCTGGTTC

TCCCAGTCCGGGAAAATCGGTATGGGTCGCAATTGGCTAATAAGTGATTGCAACACAGGA

GAAATTCTTATAAGAGCAACGAGCATTTGGGCCATGATGAATCAAAATACGAGAAGATTC

TCAAAACTTCCAAACGAGGTTCGCCAGGAGATAGCGCCTCATTTTGTTGACGCTCCTCCTG

TCATTGAAGACAATGATCGAAAATTGCATAAGTTTGATGTGAAGACTGGTGATTCCATTCG

CAAGGGTCTAACTCCGGGGTGGAATGACTTGGATGTCAATCAGCACGTAAGCAACGTGAA

GTACATTGGGTGGATTCTCGAGAGTATGCCAACAGAAGTTTTGGAGACCCAGGAGCTATG

CTCTCTCACCCTTGAATATAGGCGGGAATGCGGAAGGGACAGTGTGCTGGAGTCCGTGAC

CGCTATGAATCCCTCAAAAGTTGGAGACCGGTCTCAGTACCAGCACCTTCTACGGCTTGAG

GATGGGGCTGATATCATGAAGGGCAGAACTGAGTGGCGACCAAAGAATGCAGGAACCAA

CGGGGCGATATCAACAGGAAAGACTTCAAATGGAAACTCGGTCTCTTAG SEQ ID NO: 60

Cuphea heterophylla (Cht) FATB2e (G76D, R97L, H124L, I132S, G152S, H165L, T211N, K258N, C303R, E309G, K334T, T386A variant) coding DNA sequence codon optimized for Prototheca moriformis

AT GGTGGT GGCCGCCGCCGCCT CCTCCGCCTTCTT CCCCGT GCCCGCCTCCGGC ACCT CCC

CCAAGCCCGGCAAGTTCGGCACCTGGCTGTCCTCCTCCTCCCCCTCCTACAAGCCCAAGTC

CAACCCCTCCGGCGGCTTCCAGGTGAAGGCCAACGCCTCCGCCCACCCCAAGGCCAACGG

CTCCGCCGTGTCCCTGAAGTCCGGCTCCCTGAACACCCAGGAGGACACCTCCTCCTCCCCC

CCCCCCCAGACCTTCCTGAACCAGCTGCCCGACTGGTCCCGCCTGCTGACCGCCATCTCCA

CCGTGTTCGTGGCCGCCGAGAAGCAGCTGACCATGCTGGACCGCAAGTCCAAGCGCCCCG

ACATGCTGGTGGACTGGTTCGGCCTGGAGTCCATCGTGCAGGACGGCCTGGTGTTCCGCG

AGTCCTACTCCATCCGCTCCTACGAGATCTCCGCCGACCGCACCGCCTCCATCGAGACCGT

GATGAACCTGCTGCAGGAGACCTCCCTGAACCACTGCAAGTCCATGGGCATCCTGAACGA

CGGCTTCGGCCGCACCCCCGAGATGTGCAAGCGCGACCTGATCTGGGTGCTGACCAAGAT

GCAGATCCTGGTGAACCGCTACCCCAACTGGGGCGACACCGTGGAGATCAACTCCTGGTT

CTCCCAGTCCGGCAAGATCGGCATGGGCCGCAACTGGCTGATCTCCGACTGCAACACCGG

CGAGATCCTGATCCGCGCCACCTCCATCTGGGCCATGATGAACCAGAACACCCGCCGCTT

CTCCAAGCTGCCCAACGAGGTGCGCCAGGAGATCGCCCCCCACTTCGTGGACGCCCCCCC

CGTGATCGAGGACAACGACCGCAAGCTGCACAAGTTCGACGTGAAGACCGGCGACTCCAT

CCGCAAGGGCCTGACCCCCGGCTGGAACGACCTGGACGTGAACCAGCACGTGTCCAACGT

GAAGTACATCGGCTGGATCCTGGAGTCCATGCCCACCGAGGTGCTGGAGACCCAGGAGCT

GTGCTCCCTGACCCTGGAGTACCGCCGCGAGTGCGGCCGCGACTCCGTGCTGGAGTCCGT

GACCGCCATGAACCCCTCCAAGGTGGGCGACCGCTCCCAGTACCAGCACCTGCTGCGCCT

GGAGGACGGCGCCGACATCATGAAGGGCCGCACCGAGTGGCGCCCCAAGAACGCCGGCA

CCAACGGCGCCATCTCCACCGGCAAGACCTCCAACGGCAACTCCGTGTCCTGA SEQ ID NO: 61

Cuphea heterophylla (Cht) FATB2f (R97L, H124L, I132S, G152S, H165L, T211N variant) amino acid sequence

MWAAAASSAFFPVPASGTSPKPGKFGTWLSSSSPSYKPKSNPSGGFQVKANASAHPKANGSA

VSLKSGSLNTQEGTSSSPPPRTFLNQLPDWSRLLTAISTVFVAAEKQLTMLDRKSKRPDMLVD

WFGLESIVQDGLVFRESYSIRSYEISADRTASIETVMNLLQETSLNHCKSMGILNDGFGRTPEM

CKRDLIWVLTKMQILVNRYPNWGDTVEINSWFSQSGKIGMGRNWLISDCNTGEILIRATSIWA

MMNQKTRRFSKLPNEVRQEIAPHFVDAPPVIEDNDRKLHKFDVKTGDSICKGLTPEWNDLDV

NQHVSNVKYIGWILESMPKEVLDTQELCSLTLEYRRECGRDSVLESVTAMDPSKVGDRSQYQ HLLRLEDGTDIMKGRTEWRPKNAGTNGAISTGKTSNGNSVS* SEQ ID NO: 62

Cuphea heterophylla (Cht) FATB2f (R97L, H124L, I132S, G152S, H165L, T21 IN variant) coding DNA sequence

ATGGTGGTGGCTGCTGCAGCAAGCTCTGCATTCTTCCCTGTTCCGGCATCTGGAACCTCCC

CTAAACCCGGGAAGTTCGGGACTTGGCTATCGAGCTCGAGCCCTTCCTACAAGCCCAAGT

CAAACCCCAGTGGTGGATTTCAGGTTAAAGCAAATGCCAGTGCTCATCCTAAGGCTAACG

GTTCCGCAGTAAGTCTAAAGTCTGGCAGCCTCAACACTCAGGAGGGCACTTCGTCGTCCCC

TCCTCCTCGGACATTCCTTAACCAGTTGCCTGATTGGAGTAGGCTTCTGACTGCAATCTCG

ACCGTCTTCGTGGCGGCAGAGAAGCAGTTGACTATGCTCGATCGAAAATCTAAGAGGCCT

GACATGCTCGTGGACTGGTTTGGGTTGGAGAGTATTGTTCAGGATGGGCTCGTGTTCAGAG

AGAGTTATTCGATCAGGTCTTACGAAATAAGCGCTGATCGAACAGCCTCTATAGAGACGG

TGATGAACCTCTTGCAGGAAACATCTCTCAATCATTGTAAGAGTATGGGTATTCTCAATGA

CGGCTTTGGTCGTACCCCGGAGATGTGCAAAAGGGACCTCATTTGGGTGCTTACAAAAAT

GCAGATCTTGGTGAATCGCTATCCAAATTGGGGTGATACTGTCGAGATCAATAGCTGGTTC

TCCCAGTCCGGGAAAATCGGTATGGGTCGCAATTGGCTAATAAGTGATTGCAACACAGGA

GAAATTCTTATAAGAGCAACGAGCATTTGGGCCATGATGAATCAAAAGACGAGAAGATTC

TCAAAACTTCCAAATGAGGTTCGCCAGGAGATAGCGCCTCATTTTGTGGACGCCCCTCCTG

TCATTGAAGACAATGATCGAAAATTGCATAAGTTTGATGTGAAGACTGGTGATTCCATTTG

CAAGGGTCTAACACCGGAGTGGAACGACTTGGATGTCAATCAGCACGTAAGCAACGTGAA

GTACATTGGGTGGATTCTCGAGAGTATGCCAAAAGAAGTTTTGGACACCCAGGAGCTATG

CTCTCTCACCCTTGAATATAGGCGGGAATGCGGAAGGGACAGTGTGCTGGAGTCTGTGAC

CGCTATGGATCCCTCAAAAGTTGGAGACCGATCTCAGTACCAGCACCTTCTGCGGCTTGAA

GATGGGACTGATATCATGAAGGGCAGAACTGAGTGGCGACCAAAGAATGCAGGAACCAA

CGGGGCGATATCAACAGGAAAGACTTCAAATGGAAACTCGGTCTCTTAG SEQ ID NO: 63

Cuphea heterophylla (Cht) FATB2f (R97L, H124L, I132S, G152S, H165L, T211N variant) coding DNA sequence codon optimized for Prototheca moriformis

ATGGTGGTGGCCGCCGCCGCCTCCTCCGCCTTCTTCCCCGTGCCCGCCTCCGGCACCTCCC

CCAAGCCCGGCAAGTTCGGCACCTGGCTGTCCTCCTCCTCCCCCTCCTACAAGCCCAAGTC

CAACCCCTCCGGCGGCTTCCAGGTGAAGGCCAACGCCTCCGCCCACCCCAAGGCCAACGG

CTCCGCCGTGTCCCTGAAGTCCGGCTCCCTGAACACCCAGGAGGGCACCTCCTCCTCCCCC

CCCCCCCGCACCTTCCTGAACCAGCTGCCCGACTGGTCCCGCCTGCTGACCGCCATCTCCA

CCGTGTTCGTGGCCGCCGAGAAGCAGCTGACCATGCTGGACCGCAAGTCCAAGCGCCCCG

ACATGCTGGTGGACTGGTTCGGCCTGGAGTCCATCGTGCAGGACGGCCTGGTGTTCCGCG

AGTCCTACTCCATCCGCTCCTACGAGATCTCCGCCGACCGCACCGCCTCCATCGAGACCGT

GATGAACCTGCTGCAGGAGACCTCCCTGAACCACTGCAAGTCCATGGGCATCCTGAACGA

CGGCTTCGGCCGCACCCCCGAGATGTGCAAGCGCGACCTGATCTGGGTGCTGACCAAGAT

GCAGATCCTGGTGAACCGCTACCCCAACTGGGGCGACACCGTGGAGATCAACTCCTGGTT

CTCCCAGTCCGGCAAGATCGGCATGGGCCGCAACTGGCTGATCTCCGACTGCAACACCGG

CGAGATCCTGATCCGCGCCACCTCCATCTGGGCCATGATGAACCAGAAGACCCGCCGCTT

CTCCAAGCTGCCCAACGAGGTGCGCCAGGAGATCGCCCCCCACTTCGTGGACGCCCCCCC

CGTGATCGAGGACAACGACCGCAAGCTGCACAAGTTCGACGTGAAGACCGGCGACTCCAT

CTGCAAGGGCCTGACCCCCGAGTGGAACGACCTGGACGTGAACCAGCACGTGTCCAACGT

GAAGTACATCGGCTGGATCCTGGAGTCCATGCCCAAGGAGGTGCTGGACACCCAGGAGCT

GTGCTCCCTGACCCTGGAGTACCGCCGCGAGTGCGGCCGCGACTCCGTGCTGGAGTCCGT

GACCGCCATGGACCCCTCCAAGGTGGGCGACCGCTCCCAGTACCAGCACCTGCTGCGCCT

GGAGGACGGCACCGACATCATGAAGGGCCGCACCGAGTGGCGCCCCAAGAACGCCGGCA

CCAACGGCGCCATCTCCACCGGCAAGACCTCCAACGGCAACTCCGTGTCCTGA SEQ ID NO: 64

Cuphea heterophylla (Cht) FATB2g (A6T, A16V, S17P, G76D, R97L, H124L, I132S, S143I, G152S, A157T, H165L, T21 IN, G414A variant) amino acid sequence

MWAATASSAFFPVPVPGTSPKPGKFGTWLSSSSPSYKPKSNPSGGFQVKANASAHPKANGSA

VSLKSGSLNTQEDTSSSPPPRTFLNQLPDWSRLLTAISTVFVAAEKQLTMLDRKSKRPDMLVD

WFGLESrVQDGLVFREIYSIRSYEISADRTTSIETVMNLLQETSLNHCKSMGILNDGFGRTPEMC

KRDLIWVLTKMQILVNRYPNWGDTVEINSWFSQSGKIGMGRNWLISDCNTGEILIRATSIWAM

MNQKTRRFSKLPNEVRQEIAPHFVDAPPVIEDNDRKLHKFDVKTGDSICKGLTPEWNDLDVN

QHVSNVKYIGWILESMPKEVLDTQELCSLTLEYRRECGRDSVLESVTAMDPSKVGDRSQYQH LLRLEDGTDIMKGRTEWRPKNAGTNGAISTGKTSNANSVS* SEQ ID NO: 65

Cuphea heterophylla (Cht) FATB2g (A6T, A16V, S17P, G76D, R97L, H124L, I132S, S143I, G152S, A157T, H165L, T21 IN, G414A variant) coding DNA sequence

ATGGTGGTGGCTGCTACAGCAAGTTCTGCATTCTTCCCTGTTCCTGTACCTGGAACCTCCC

CTAAACCCGGAAAGTTCGGGACTTGGCTATCGAGCTCGAGCCCTTCCTACAAGCCCAAGT

CAAACCCCAGTGGTGGATTTCAGGTTAAGGCAAATGCCAGTGCTCATCCTAAGGCTAACG

GTTCTGCAGTAAGTCTAAAGTCTGGCAGCCTCAACACTCAGGAGGACACTTCGTCGTCCCC

TCCTCCTCGGACATTCCTTAACCAGTTGCCTGATTGGAGTAGGCTTCTGACTGCAATCTCG

ACCGTCTTCGTGGCGGCAGAGAAGCAGTTGACTATGCTCGATCGAAAATCTAAGAGGCCT

GACATGCTCGTGGACTGGTTTGGGTTGGAGAGTATTGTTCAGGATGGGCTCGTGTTCAGAG

AGATTTATTCGATCAGGTCTTACGAAATAAGCGCTGATCGAACAACCTCTATAGAGACGG

TGATGAACCTCTTGCAGGAAACATCTCTCAATCATTGTAAGAGTATGGGTATTCTCAATGA

CGGCTTTGGTCGTACCCCGGAGATGTGCAAAAGGGACCTCATTTGGGTGCTTACAAAAAT

GCAGATCTTGGTGAATCGCTATCCAAATTGGGGTGATACTGTCGAGATCAATAGCTGGTTC

TCCCAGTCCGGGAAAATCGGTATGGGTCGCAATTGGCTAATAAGTGATTGCAACACAGGA

GAAATTCTTATAAGAGCAACGAGCATTTGGGCCATGATGAATCAAAAGACGAGAAGATTC

TCAAAACTTCCAAACGAGGTTCGCCAGGAGATAGCGCCTCATTTTGTGGACGCCCCTCCTG

TCATTGAAGACAATGATCGAAAATTGCATAAGTTTGATGTGAAGACTGGTGATTCCATTTG

CAAGGGTCTAACACCGGAGTGGAATGACTTGGATGTCAATCAGCACGTAAGCAACGTGAA

GTACATTGGGTGGATTCTCGAGAGTATGCCAAAAGAAGTTTTGGACACCCAGGAGCTATG

CTCTCTCACCCTTGAATATAGGCGGGAATGCGGAAGGGACAGTGTGCTGGAGTCTGTGAC

CGCTATGGATCCCTCAAAAGTTGGAGACCGATCTCAGTACCAGCACCTTCTGCGGCTTGAA

GATGGGACTGATATCATGAAGGGCAGAACTGAGTGGCGACCAAAGAATGCAGGAACCAA

CGGGGCGATATCAACAGGAAAGACTTCAAATGCAAACTCGGTCTCTTAG SEQ ID NO: 66

Cuphea heterophylla (Cht) FATB2g (A6T, A16V, S17P, G76D, R97L, H124L, I132S, S143I, G152S, A157T, H165L, T21 IN, G414A variant) coding DNA sequence codon optimized for Prototheca moriformis

ATGGTGGTGGCCGCCACCGCCTCCTCCGCCTTCTTCCCCGTGCCCGTGCCCGGCACCTCCC

CCAAGCCCGGCAAGTTCGGCACCTGGCTGTCCTCCTCCTCCCCCTCCTACAAGCCCAAGTC

CAACCCCTCCGGCGGCTTCCAGGTGAAGGCCAACGCCTCCGCCCACCCCAAGGCCAACGG

CTCCGCCGTGTCCCTGAAGTCCGGCTCCCTGAACACCCAGGAGGACACCTCCTCCTCCCCC

CCCCCCCGCACCTTCCTGAACCAGCTGCCCGACTGGTCCCGCCTGCTGACCGCCATCTCCA

CCGTGTTCGTGGCCGCCGAGAAGCAGCTGACCATGCTGGACCGCAAGTCCAAGCGCCCCG

ACATGCTGGTGGACTGGTTCGGCCTGGAGTCCATCGTGCAGGACGGCCTGGTGTTCCGCG

AGATCTACTCCATCCGCTCCTACGAGATCTCCGCCGACCGCACCACCTCCATCGAGACCGT

GATGAACCTGCTGCAGGAGACCTCCCTGAACCACTGCAAGTCCATGGGCATCCTGAACGA

CGGCTTCGGCCGCACCCCCGAGATGTGCAAGCGCGACCTGATCTGGGTGCTGACCAAGAT

GCAGATCCTGGTGAACCGCTACCCCAACTGGGGCGACACCGTGGAGATCAACTCCTGGTT

CTCCCAGTCCGGCAAGATCGGCATGGGCCGCAACTGGCTGATCTCCGACTGCAACACCGG

CGAGATCCTGATCCGCGCCACCTCCATCTGGGCCATGATGAACCAGAAGACCCGCCGCTT

CTCCAAGCTGCCCAACGAGGTGCGCCAGGAGATCGCCCCCCACTTCGTGGACGCCCCCCC

CGTGATCGAGGACAACGACCGCAAGCTGCACAAGTTCGACGTGAAGACCGGCGACTCCAT

CTGCAAGGGCCTGACCCCCGAGTGGAACGACCTGGACGTGAACCAGCACGTGTCCAACGT

GAAGTACATCGGCTGGATCCTGGAGTCCATGCCCAAGGAGGTGCTGGACACCCAGGAGCT

GTGCTCCCTGACCCTGGAGTACCGCCGCGAGTGCGGCCGCGACTCCGTGCTGGAGTCCGT

GACCGCCATGGACCCCTCCAAGGTGGGCGACCGCTCCCAGTACCAGCACCTGCTGCGCCT

GGAGGACGGCACCGACATCATGAAGGGCCGCACCGAGTGGCGCCCCAAGAACGCCGGCA

CCAACGGCGCCATCTCCACCGGCAAGACCTCCAACGCCAACTCCGTGTCCTGA SEQ ID NO: 67

Cuphea heterophylla (Cht) FATB3aamino acid sequence

MVATAASSAFFPVPSPDTSSRPGKLGNGSSSLRPLKPKFVANAGLQVKANASAPPKINGSSVSL

KSCSLKTHEDTPSAPPPRTFINQLPDWSMLLAAITTVFLAAEKQWMMLDWKPKRPDMLVDPF

GLGRIVQDGLVFRQNFSIRSYEIGADRTASIETVMNHLQETALNHVKSAGLLNEGFGRTPEMY

KRDLIWWAKMQVMVNRYPTWGDTVEVNTWVAKSGKNGMRRDWLISDCNTGEILTRASSV

WVMMNQKTRKLSKIPDEVRHEIEPHFVDSAPVIEDDDWKLPKLDEKTADSIRKGLTPKWNDL

DVNQHVNNVKYIGWILESTPPEVLETQELCSLTLEYRRECGRESVLESLTAVDPSGKGFGPQFQ HLLRLEDGGEIVKGRTEWRPKTAGINGTLASGETSPGNS* SEQ ID NO: 68

Cuphea heterophylla (Cht) FATB3a coding DNA sequence

ATGGTGGCCACCGCTGCAAGTTCTGCATTCTTCCCGGTGCCGTCCCCGGACACCTCCTCTA

GACCGGGAAAGCTCGGAAATGGGTCATCAAGCTTGAGGCCCCTCAAGCCCAAATTTGTTG

CCAATGCTGGGCTGCAGGTTAAGGCAAACGCCAGTGCCCCTCCTAAGATCAATGGTTCCT

CGGTCAGTCTAAAGTCTTGCAGTCTCAAGACTCATGAAGACACTCCTTCAGCTCCTCCTCC

GCGGACTTTTATCAACCAGTTGCCTGATTGGAGCATGCTTCTTGCTGCAATCACTACTGTC

TTCTTGGCAGCAGAGAAGCAGTGGATGATGCTTGATTGGAAACCAAAGAGGCCTGACATG

CTTGTGGACCCGTTCGGATTGGGAAGGATTGTTCAGGATGGGCTTGTGTTCAGGCAGAATT

TTTCGATTAGGTCCTATGAAATAGGCGCTGATCGCACTGCATCCATAGAGACGGTGATGA

ACCACTTGCAGGAAACGGCTCTCAATCATGTTAAGAGTGCGGGGCTTCTTAATGAAGGCT

TTGGTCGTACTCCTGAGATGTATAAAAGGGACCTTATTTGGGTTGTCGCGAAAATGCAGGT

CATGGTTAACCGCTATCCTACTTGGGGTGACACGGTTGAAGTGAATACTTGGGTTGCCAAG

TCAGGGAAAAATGGTATGCGTCGTGATTGGCTCATAAGTGATTGCAATACAGGAGAAATT

CTTACAAGGGCATCAAGTGTGTGGGTCATGATGAATCAAAAGACAAGAAAATTGTCAAAG

ATTCCAGATGAGGTTCGGCATGAGATAGAGCCTCATTTTGTGGACTCTGCTCCCGTCATTG

AAGACGATGACTGGAAACTTCCCAAGCTGGATGAGAAAACTGCTGACTCCATCCGCAAGG

GT CTAACT CCGAAGT GGAATGACTTGGATGTCAATC AGCACGTC AAC AACGT GAAGT AC A

TTGGGTGGATTCTTGAGAGTACTCCACCAGAAGTTCTGGAGACCCAGGAGTTATGTTCCCT

TACCCTGGAATACAGGCGGGAATGCGGAAGGGAGAGTGTGCTGGAGTCCCTCACTGCTGT

GGACCCCTCTGGAAAGGGCTTTGGGCCCCAGTTTCAGCACCTTCTGAGGCTTGAGGATGG

AGGTGAGATCGTAAAGGGGAGAACTGAGTGGCGACCCAAGACTGCAGGTATCAATGGGA

CGATTGCATCTGGGGAGACCTCACCTGGAAACTCTTAG SEQ ID NO: 69

Cuphea heterophylla (Cht) FATB3a coding DNA sequence codon optimized for Pro to theca moriformis

ATGGTGGCCACCGCCGCCTCCTCCGCCTTCTTCCCCGTGCCCTCCCCCGACACCTCCTCCCG

CCCCGGCAAGCTGGGCAACGGCTCCTCCTCCCTGCGCCCCCTGAAGCCCAAGTTCGTGGCC

AACGCCGGCCTGCAGGTGAAGGCCAACGCCTCCGCCCCCCCCAAGATCAACGGCTCCTCC

GTGTCCCTGAAGTCCTGCTCCCTGAAGACCCACGAGGACACCCCCTCCGCCCCCCCCCCCC

GCACCTTCATCAACCAGCTGCCCGACTGGTCCATGCTGCTGGCCGCCATCACCACCGTGTT

CCTGGCCGCCGAGAAGCAGTGGATGATGCTGGACTGGAAGCCCAAGCGCCCCGACATGCT

GGTGGACCCCTTCGGCCTGGGCCGCATCGTGCAGGACGGCCTGGTGTTCCGCCAGAACTT

CTCCATCCGCTCCTACGAGATCGGCGCCGACCGCACCGCCTCCATCGAGACCGTGATGAA

CCACCTGCAGGAGACCGCCCTGAACCACGTGAAGTCCGCCGGCCTGCTGAACGAGGGCTT

CGGCCGCACCCCCGAGATGTACAAGCGCGACCTGATCTGGGTGGTGGCCAAGATGCAGGT

GATGGTGAACCGCTACCCCACCTGGGGCGACACCGTGGAGGTGAACACCTGGGTGGCCAA

GTCCGGCAAGAACGGCATGCGCCGCGACTGGCTGATCTCCGACTGCAACACCGGCGAGAT

CCTGACCCGCGCCTCCTCCGTGTGGGTGATGATGAACCAGAAGACCCGCAAGCTGTCCAA

GATCCCCGACGAGGTGCGCCACGAGATCGAGCCCCACTTCGTGGACTCCGCCCCCGTGAT

CGAGGACGACGACTGGAAGCTGCCCAAGCTGGACGAGAAGACCGCCGACTCCATCCGCA

AGGGCCTGACCCCCAAGTGGAACGACCTGGACGTGAACCAGCACGTGAACAACGTGAAG

TACATCGGCTGGATCCTGGAGTCCACCCCCCCCGAGGTGCTGGAGACCCAGGAGCTGTGC

TCCCTGACCCTGGAGTACCGCCGCGAGTGCGGCCGCGAGTCCGTGCTGGAGTCCCTGACC

GCCGTGGACCCCTCCGGCAAGGGCTTCGGCCCCCAGTTCCAGCACCTGCTGCGCCTGGAG

GACGGCGGCGAGATCGTGAAGGGCCGCACCGAGTGGCGCCCCAAGACCGCCGGCATCAA

CGGCACCATCGCCTCCGGCGAGACCTCCCCCGGCAACTCCTGA SEQ ID NO: 70

Cuphea heterophylla (Cht) FATB3b (C67G, H72Q, L128F, N179I variant) amino acid sequence

MVATAASSAFFPVPSPDTSSRPGKLGNGSSSLRPLKPKFVANAGLQVKANASAPPKINGSSVSL

KSGSLKTQEDTPSAPPPRTFINQLPDWSMLLAAITTVFLAAEKQWMMLDWKPKRPDMLVDPF

GFGRIVQDGLVFRQNFSIRSYEIGADRTASIETVMNHLQETALNHVKSAGLLIEGFGRTPEMYK

RDLIWWAKMQVMVNRYPTWGDTVEVNTWVAKSGKNGMRRDWLISDCNTGEILTRASSVW

VMMNQKTRKLSKIPDEVRHEIEPHFVDSAPVIEDDDWKLPKLDEKTADSIRKGLTPKWNDLD

VNQHVNNVKYIGWILESTPPEVLETQELCSLTLEYRRECGRESVLESLTAVDPSGKGFGPQFQH LLRLEDGGEIVKGRTE WRPKTAGINGTIASGET SPGN S * SEQ ID NO: 71

Cuphea heterophylla (Cht) FATB3b (C67G, H72Q, L128F, N179I variant) coding DNA sequence

ATGGTGGCCACCGCTGCAAGTTCTGCATTCTTCCCGGTGCCATCCCCGGACACCTCCTCTA

GACCGGGAAAGCTCGGAAATGGGTCATCAAGCTTGAGGCCCCTCAAGCCCAAATTTGTTG

CCAATGCTGGGCTGCAGGTTAAGGCAAACGCCAGTGCCCCTCCTAAGATCAATGGTTCCT

CGGTCAGTCTAAAGTCTGGCAGTCTCAAGACTCAGGAAGACACTCCTTCGGCTCCTCCTCC

GCGGACTTTTATCAACCAGTTGCCTGATTGGAGCATGCTTCTTGCTGCAATCACTACTGTC

TTCTTGGCAGCAGAGAAGCAGTGGATGATGCTTGATTGGAAACCAAAGAGGCCTGACATG

CTTGTGGACCCGTTCGGATTTGGAAGGATTGTTCAGGATGGGCTTGTGTTCAGGCAGAATT

TTTCGATTAGGTCCTATGAAATAGGCGCTGATCGCACTGCATCTATAGAGACGGTGATGA

ACCACTTGCAGGAAACGGCTCTCAATCATGTTAAGAGTGCGGGGCTTCTTATTGAAGGCTT

TGGTCGTACTCCTGAGATGTATAAAAGGGACCTTATTTGGGTTGTCGCGAAAATGCAGGTC

AT GGTT AACCGCT ATCCTACTTGGGGT GAC ACGGTT GAAGT GAAT ACTTGGGTTGCC AAGT

CAGGGAAAAATGGTATGCGTCGTGATTGGCTCATAAGTGATTGCAATACAGGAGAAATTC

TTACTAGAGCATCAAGTGTGTGGGTCATGATGAATCAAAAGACAAGAAAATTGTCAAAGA

TTCCAGATGAGGTTCGGCATGAGATAGAGCCTCATTTTGTGGACTCTGCTCCCGTCATTGA

AGACGATGACTGGAAACTTCCCAAGCTGGATGAGAAAACTGCTGACTCCATCCGCAAGGG

TCTAACTCCGAAGTGGAATGACTTGGATGTCAATCAGCACGTCAACAACGTGAAGTACAT

TGGGTGGATTCTTGAGAGTACTCCACCAGAAGTTCTGGAGACCCAGGAGTTATGTTCCCTT

ACCCTGGAATACAGGCGGGAATGCGGAAGGGAGAGTGTGCTGGAGTCCCTCACTGCTGTG

GACCCCTCTGGAAAGGGCTTTGGGCCCCAGTTTCAGCACCTTCTGAGGCTTGAGGATGGA

GGTGAGATCGTAAAGGGGAGAACTGAGTGGCGACCCAAGACTGCAGGTATCAATGGGAC

GATTGCATCTGGGGAGACCTCACCTGGAAACTCTTAG SEQ ID NO: 72

Cuphea heterophylla (Cht) FATB3b (C67G, H72Q, L128F, N179I variant) coding DNA sequence codon optimized for Prototheca moriformis

ATGGTGGCCACCGCCGCCTCCTCCGCCTTCTTCCCCGTGCCCTCCCCCGACACCTCCTCCCG

CCCCGGCAAGCTGGGCAACGGCTCCTCCTCCCTGCGCCCCCTGAAGCCCAAGTTCGTGGCC

AACGCCGGCCTGCAGGTGAAGGCCAACGCCTCCGCCCCCCCCAAGATCAACGGCTCCTCC

GT GTCCCT GAAGT CCGGCT CCCTGAAGACCCAGGAGG AC ACCCCCT CCGCCCCCCCCCCCC

GCACCTTCATCAACCAGCTGCCCGACTGGTCCATGCTGCTGGCCGCCATCACCACCGTGTT

CCTGGCCGCCGAGAAGCAGTGGATGATGCTGGACTGGAAGCCCAAGCGCCCCGACATGCT

GGTGGACCCCTTCGGCTTCGGCCGCATCGTGCAGGACGGCCTGGTGTTCCGCCAGAACTTC

TCCATCCGCTCCTACGAGATCGGCGCCGACCGCACCGCCTCCATCGAGACCGTGATGAAC

CACCTGCAGGAGACCGCCCTGAACCACGTGAAGTCCGCCGGCCTGCTGATCGAGGGCTTC

GGCCGCACCCCCGAGATGTACAAGCGCGACCTGATCTGGGTGGTGGCCAAGATGCAGGTG

ATGGTGAACCGCTACCCCACCTGGGGCGACACCGTGGAGGTGAACACCTGGGTGGCCAAG

TCCGGCAAGAACGGCATGCGCCGCGACTGGCTGATCTCCGACTGCAACACCGGCGAGATC

CTGACCCGCGCCTCCTCCGTGTGGGTGATGATGAACCAGAAGACCCGCAAGCTGTCCAAG

ATCCCCGACGAGGTGCGCCACGAGATCGAGCCCCACTTCGTGGACTCCGCCCCCGTGATC

GAGGACGACGACTGGAAGCTGCCCAAGCTGGACGAGAAGACCGCCGACTCCATCCGCAA

GGGCCTGACCCCCAAGTGGAACGACCTGGACGTGAACCAGCACGTGAACAACGTGAAGT

ACATCGGCTGGATCCTGGAGTCCACCCCCCCCGAGGTGCTGGAGACCCAGGAGCTGTGCT

CCCTGACCCTGGAGTACCGCCGCGAGTGCGGCCGCGAGTCCGTGCTGGAGTCCCTGACCG

CCGTGGACCCCTCCGGCAAGGGCTTCGGCCCCCAGTTCCAGCACCTGCTGCGCCTGGAGG

ACGGCGGCGAGATCGTGAAGGGCCGCACCGAGTGGCGCCCCAAGACCGCCGGCATCAAC

GGCACCATCGCCTCCGGCGAGACCTCCCCCGGCAACTCCTGA SEQ ID NO: 73

Cuphea viscosissima (Cvis) FATB1 amino acid sequence

MVAAAATSAFFPVPAPGTSPKPGKSGNWPSSLSPTFKPKSIPNGGFQVKANASAHPKANGSAV

NLKSGSLNTQEDTS SSPPPRAFLNQLPDW SMLLTAITTVFVAAEKQ WTMLDRKSKRPDMLVD

SVGLKSrVRDGLVSRHSFSIRSYEIGADRTASIETLMNHLQETTINHCKSLGLHNDGFGRTPGM

CKNDLIWVLTKMQIMVNRYPTWGDTVEINTWFSQSGKIGMASDWLISDCNTGEILIRATSVW

AMMNQKTRRFSRLPYEVRQELTPHFVDSPHVIEDNDQKLRKFDVKTGDSIRKGLTPRWNDLD

VNQHVSNVKYIGWILESMPIEVLETQELCSLTVEYRRECGMDSVLESVTAVDPSENGGRSQYK

HLLRLEDGTDIVKSRTEWRPKNAGTNGAISTSTAKTSNGNSVS SEQ ID NO: 74

Cuphea viscosissima (Cvis) FATB1 coding DNA sequence codon optimized for Prototheca moriformis

ATGGTGGCCGCCGCCGCCACCTCCGCCTTCTTCCCCGTGCCCGCCCCCGGCACCTCCCCCA

AGCCCGGCAAGTCCGGCAACTGGCCCTCCTCCCTGTCCCCCACCTTCAAGCCCAAGTCCAT

CCCCAACGGCGGCTTCCAGGTGAAGGCCAACGCCTCCGCCCACCCCAAGGCCAACGGCTC

CGCCGTGAACCTGAAGTCCGGCTCCCTGAACACCCAGGAGGACACCTCCTCCTCCCCCCCC

CCCCGCGCCTTCCTGAACCAGCTGCCCGACTGGTCCATGCTGCTGACCGCCATCACCACCG

TGTTCGTGGCCGCCGAGAAGCAGTGGACCATGCTGGACCGCAAGTCCAAGCGCCCCGACA

TGCTGGTGGACTCCGTGGGCCTGAAGTCCATCGTGCGCGACGGCCTGGTGTCCCGCCACTC

CTTCTCCATCCGCTCCTACGAGATCGGCGCCGACCGCACCGCCTCCATCGAGACCCTGATG

AACCACCTGCAGGAGACCACCATCAACCACTGCAAGTCCCTGGGCCTGCACAACGACGGC

TTCGGCCGCACCCCCGGCATGTGCAAGAACGACCTGATCTGGGTGCTGACCAAGATGCAG

ATCATGGTGAACCGCTACCCCACCTGGGGCGACACCGTGGAGATCAACACCTGGTTCTCC

C AGTCCGGC AAGAT CGGCAT GGCCTCCGACTGGCTGAT CT CCGACT GC AAC ACCGGCGAG

ATCCTGATCCGCGCCACCTCCGTGTGGGCCATGATGAACCAGAAGACCCGCCGCTTCTCCC

GCCTGCCCTACGAGGTGCGCCAGGAGCTGACCCCCCACTTCGTGGACTCCCCCCACGTGAT

CGAGGACAACGACCAGAAGCTGCGCAAGTTCGACGTGAAGACCGGCGACTCCATCCGCA

AGGGCCTGACCCCCCGCTGGAACGACCTGGACGTGAACCAGCACGTGTCCAACGTGAAGT

ACATCGGCTGGATCCTGGAGTCCATGCCCATCGAGGTGCTGGAGACCCAGGAGCTGTGCT

CCCTGACCGTGGAGTACCGCCGCGAGTGCGGCATGGACTCCGTGCTGGAGTCCGTGACCG

CCGTGGACCCCTCCGAGAACGGCGGCCGCTCCCAGTACAAGCACCTGCTGCGCCTGGAGG

ACGGCACCGACATCGTGAAGTCCCGCACCGAGTGGCGCCCCAAGAACGCCGGCACCAAC

GGCGCCATCTCCACCTCCACCGCCAAGACCTCCAACGGCAACTCCGTGTCCTGA SEQ ID NO: 75

Cuphea viscosissima (Cvis) FATB2 amino acid sequence

MVATAASSAFFPVPSADTSSRPGKLGNGPSSFSPLKPKSIPNGGLQVKASASAPPKINGSSVGLK

SGGLKTHDDAPSAPPPRTFINQLPDWSMLLAAITTAFLAAEKQWMMLDRKPKRLDMLEDPFG

LGRWQDGLVFRQNFSIRSYEIGADRTASIETVMNHLQETALNHVKTAGLSNDGFGRTPEMYK

RDLIWWAKMQVMVNRYPTWGDTVEVNTWVAKSGKNGMRRDWLISDCNTGEILTRASSVW

VMMNQKTRKLSKIPDEVRREIEPHFVDSAPVIEDDDRKLPKLDEKSADSIRKGLTPRWNDLDV

NQHVNNAKYIGWILESTPPEVLETQELCSLTLEYRRECGRESVLESLTAVDPSGEGYGSQFQHL

LRLEDGGEIVKGRTE WRPKNAGINGWPSEE S SPGDY S SEQ ID NO: 76

Cuphea viscosissima (Cvis) FATB2 coding DNA sequence codon optimized for Prototheca moriformis

ATGGTGGCCACCGCCGCCTCCTCCGCCTTCTTCCCCGTGCCCTCCGCCGACACCTCCTCCC

GCCCCGGCAAGCTGGGCAACGGCCCCTCCTCCTTCTCCCCCCTGAAGCCCAAGTCCATCCC

CAACGGCGGCCTGCAGGTGAAGGCCTCCGCCTCCGCCCCCCCCAAGATCAACGGCTCCTC

CGTGGGCCTGAAGTCCGGCGGCCTGAAGACCCACGACGACGCCCCCTCCGCCCCCCCCCC

CCGCACCTTCATCAACCAGCT GCCCGACT GGTCC ATGCT GCT GGCCGCC ATC ACC ACCGCC

TTCCTGGCCGCCGAGAAGCAGTGGATGATGCTGGACCGCAAGCCCAAGCGCCTGGACATG

CTGGAGGACCCCTTCGGCCTGGGCCGCGTGGTGCAGGACGGCCTGGTGTTCCGCCAGAAC

TTCTCCATCCGCTCCTACGAGATCGGCGCCGACCGCACCGCCTCCATCGAGACCGTGATGA

ACCACCTGCAGGAGACCGCCCTGAACCACGTGAAGACCGCCGGCCTGTCCAACGACGGCT

TCGGCCGCACCCCCGAGATGTACAAGCGCGACCTGATCTGGGTGGTGGCCAAGATGCAGG

TGATGGTGAACCGCTACCCCACCTGGGGCGACACCGTGGAGGTGAACACCTGGGTGGCCA

AGTCCGGCAAGAACGGCATGCGCCGCGACTGGCTGATCTCCGACTGCAACACCGGCGAGA

TCCTGACCCGCGCCTCCTCCGTGTGGGTGATGATGAACCAGAAGACCCGCAAGCTGTCCA

AGATCCCCGACGAGGTGCGCCGCGAGATCGAGCCCCACTTCGTGGACTCCGCCCCCGTGA

TCGAGGACGACGACCGCAAGCTGCCCAAGCTGGACGAGAAGTCCGCCGACTCCATCCGCA

AGGGCCTGACCCCCCGCTGGAACGACCTGGACGTGAACCAGCACGTGAACAACGCCAAGT

ACATCGGCTGGATCCTGGAGTCCACCCCCCCCGAGGTGCTGGAGACCCAGGAGCTGTGCT

CCCTGACCCTGGAGTACCGCCGCGAGTGCGGCCGCGAGTCCGTGCTGGAGTCCCTGACCG

CCGTGGACCCCTCCGGCGAGGGCTACGGCTCCCAGTTCCAGCACCTGCTGCGCCTGGAGG

ACGGCGGCGAGATCGTGAAGGGCCGCACCGAGTGGCGCCCCAAGAACGCCGGCATCAAC

GGCGTGGTGCCCTCCGAGGAGTCCTCCCCCGGCGACTACTCCTGA SEQ ID NO: 77

Cuphea viscosissima (Cvis) FATB3 amino acid sequence

MVAAAASSAFFSFPTPGTSPKPGKFGNWPSSLSIPFNPKSNHNGGIQVKANASAHPKANGSAVS

LKAGSLETQEDTSSPSPPPRTFISQLPDWSMLVSAITTVFVAAEKQWTMLDRKSKRPDVLVEPF

VQDGVSFRQSFSIRSYEIGVDRTASIETLMNIFQETSLNHCKSLGLLNDGFGRTPEMCKRDLIW

WTKMQIEVNRYPTWGDTIEVTTWVSESGKNGMSRDWLISDCHSGEILIRATSVWAMMNQK

TRRLSKIPDEVRQEIVPYFVDSAPVIEDDRKLHKLDVKTGDSIRNGLTPRWNDFDVNQHVNNV

KYIAWLLKSVPTEVFETQELCGLTLEYRRECRRDSVLESVTAMDPSKEGDRSLYQHLLRLENG ADIALGRTE WRPKNAGAT GAV STGKTSNGN S VS SEQ ID NO: 78

Cuphea viscosissima (Cvis) FATB3 coding DNA sequence codon optimized for Prototheca moriformis

ATGGTGGCCGCCGCCGCCTCCTCCGCCTTCTTCTCCTTCCCCACCCCCGGCACCTCCCCCAA

GCCCGGCAAGTTCGGCAACTGGCCCTCCTCCCTGTCCATCCCCTTCAACCCCAAGTCCAAC

CACAACGGCGGCATCCAGGTGAAGGCCAACGCCTCCGCCCACCCCAAGGCCAACGGCTCC

GCCGTGTCCCTGAAGGCCGGCTCCCTGGAGACCCAGGAGGACACCTCCTCCCCCTCCCCCC

CCCCCCGCACCTTCATCTCCCAGCTGCCCGACTGGTCCATGCTGGTGTCCGCCATCACCAC

CGTGTTCGTGGCCGCCGAGAAGCAGTGGACCATGCTGGACCGCAAGTCCAAGCGCCCCGA

CGTGCTGGTGGAGCCCTTCGTGCAGGACGGCGTGTCCTTCCGCCAGTCCTTCTCCATCCGC

TCCTACGAGATCGGCGTGGACCGCACCGCCTCCATCGAGACCCTGATGAACATCTTCCAG

GAGACCTCCCTGAACCACTGCAAGTCCCTGGGCCTGCTGAACGACGGCTTCGGCCGCACC

CCCGAGATGTGCAAGCGCGACCTGATCTGGGTGGTGACCAAGATGCAGATCGAGGTGAAC

CGCTACCCCACCTGGGGCGACACCATCGAGGTGACCACCTGGGTGTCCGAGTCCGGCAAG

AACGGCATGTCCCGCGACTGGCTGATCTCCGACTGCCACTCCGGCGAGATCCTGATCCGC

GCCACCTCCGTGTGGGCCATGATGAACCAGAAGACCCGCCGCCTGTCCAAGATCCCCGAC

GAGGTGCGCCAGGAGATCGTGCCCTACTTCGTGGACTCCGCCCCCGTGATCGAGGACGAC

CGCAAGCTGCACAAGCTGGACGTGAAGACCGGCGACTCCATCCGCAACGGCCTGACCCCC

CGCTGGAACGACTTCGACGTGAACCAGCACGTGAACAACGTGAAGTACATCGCCTGGCTG

CTGAAGTCCGTGCCCACCGAGGTGTTCGAGACCCAGGAGCTGTGCGGCCTGACCCTGGAG

TACCGCCGCGAGTGCCGCCGCGACTCCGTGCTGGAGTCCGTGACCGCCATGGACCCCTCC

AAGGAGGGCGACCGCTCCCTGTACCAGCACCTGCTGCGCCTGGAGAACGGCGCCGACATC

GCCCTGGGCCGCACCGAGTGGCGCCCCAAGAACGCCGGCGCCACCGGCGCCGTGTCCACC

GGCAAGACCTCCAACGGCAACTCCGTGTCCTGA SEQ ID NO: 79

Cuphea calcarata (Ccalc) FATB1 amino acid sequence

MVAASASSAFFSVPTPGTSPKPGKFGNWPSSLSVPFKPRSNNSGGFQVKANASAHPKANGSAV

SLKSGSLETQEDNSSSSRPPRTFIKQLPDWSMLLSAITTVFVAAEKQWTMFDRKSKRSDMLVD

PFWDRTVQDGVLFRQSFSIRSYEIGADRTASIETLMNIFQETSLNHCKSMGLLYEGFGRTPEMC

KRDLIWWTKIHIKVNRYPTWGDTIEVTTWVSESGKNGMGRDWLISDCHTGEILIRATSVWA

MMNQTTRRLSKFPYELRQEIAPHFVDSDPVIEDNRRLLNFDVKTGDSIRKGLTPRWNDLDVNQ

HVNNVKYIGWILESVPTEVFDTRELCGLTLEYRQECGRGSVLESVTAMDPSKEGDRSLYQHLL

RLEDGTDIVKGRTE WRPKNAGTNGPV STRKTTNGS S VS SEQ ID NO: 80

Cuphea calcarata (Ccalc) FATB1 coding DNA sequence

ATGGTGGCTGCTTCAGCAAGTTCTGCATTCTTCTCCGTCCCAACCCCGGGAACCTCTCCTA

AACCCGGGAAGTTCGGCAATTGGCCATCGAGCTTGAGCGTCCCATTCAAGCCCAGATCAA

ACAACAGTGGCGGCTTTCAGGTTAAGGCAAACGCCAGTGCTCATCCTAAGGCTAACGGTT

CTGCAGTAAGTCTAAAGTCTGGGAGCCTCGAGACTCAGGAGGACAATTCGTCGTCGTCTC

GTCCTCCTCGGACTTTCATTAAACAGTTGCCGGACTGGAGTATGCTTCTTTCCGCGATCAC

AACCGTCTTCGTGGCGGCTGAGAAGCAGTGGACGATGTTTGATCGGAAATCTAAGAGGTC

TGACATGCTCGTGGACCCGTTTGTGGTTGACAGGATTGTTCAGGATGGGGTTCTGTTCAGA

CAGAGTTTTTCGATTAGGTCTTACGAAATAGGCGCTGATCGAACAGCCTCTATTGAGACGC

TGATGAACATCTTCCAGGAAACATCTCTCAATCATTGTAAGAGTATGGGTCTTCTCTATGA

AGGCTTTGGTCGTACTCCTGAGATGTGTAAGAGGGACCTCATTTGGGTGGTTACGAAAAT

ACATATCAAGGTGAATCGCTATCCGACTTGGGGTGATACTATCGAGGTCACTACTTGGGTC

TCCGAGTCGGGCAAAAACGGTATGGGTCGCGATTGGCTGATAAGTGATTGCCATACAGGA

GAAATTCTTATAAGAGCAACGAGTGTGTGGGCTATGATGAATCAAACGACGAGAAGATTG

TCGAAATTTCCATATGAGCTTCGACAGGAGATAGCGCCACATTTTGTGGACTCGGATCCTG

TCATTGAAGACAATCGAAGATTGCTCAACTTTGATGTGAAGACGGGTGATTCCATTCGCA

AGGGTCTAACTCCAAGGTGGAATGACTTGGATGTCAATCAGCACGTTAACAATGTGAAGT

ACATTGGGTGGATTCTCGAGAGTGTTCCAACAGAAGTTTTCGATACCCGGGAGCTATGCG

GCCTCACCCTTGAGTATAGGCAGGAATGCGGAAGAGGAAGTGTGCTGGAGTCCGTGACCG

CTATGGATCCCTCAAAAGAGGGAGACCGGTCTCTGTACCAGCACCTTCTTCGGCTTGAGG

ATGGGACTGATATCGTGAAGGGCAGAACCGAGTGGCGGCCAAAGAATGCAGGAACCAAT

GGGCCAGTATCAACAAGAAAGACTACAAATGGAAGCTCAGTCTCTTAG SEQ ID NO: 81

Cuphea calcarata (Ccalc) FATB1 coding DNA sequence codon optimized for Prototheca moriformis

ATGGTGGCCGCCTCCGCCTCCTCCGCCTTCTTCTCCGTGCCCACCCCCGGCACCTCCCCCA

AGCCCGGCAAGTTCGGCAACT GGCCCT CCT CCCTGT CCGT GCCCTT C AAGCCCCGCTCC AA

CAACTCCGGCGGCTTCCAGGTGAAGGCCAACGCCTCCGCCCACCCCAAGGCCAACGGCTC

CGCCGTGTCCCTGAAGTCCGGCTCCCTGGAGACCCAGGAGGACAACTCCTCCTCCTCCCGC

CCCCCCCGCACCTTCATCAAGCAGCTGCCCGACTGGTCCATGCTGCTGTCCGCCATCACCA

CCGTGTTCGTGGCCGCCGAGAAGCAGTGGACCATGTTCGACCGCAAGTCCAAGCGCTCCG

ACATGCTGGTGGACCCCTTCGTGGTGGACCGCATCGTGCAGGACGGCGTGCTGTTCCGCC

AGTCCTTCTCCATCCGCTCCTACGAGATCGGCGCCGACCGCACCGCCTCCATCGAGACCCT

GATGAACATCTTCCAGGAGACCTCCCTGAACCACTGCAAGTCCATGGGCCTGCTGTACGA

GGGCTTCGGCCGCACCCCCGAGATGTGCAAGCGCGACCTGATCTGGGTGGTGACCAAGAT

CCACATCAAGGTGAACCGCTACCCCACCTGGGGCGACACCATCGAGGTGACCACCTGGGT

GTCCGAGTCCGGCAAGAACGGCATGGGCCGCGACTGGCTGATCTCCGACTGCCACACCGG

CGAGATCCTGATCCGCGCCACCTCCGTGTGGGCCATGATGAACCAGACCACCCGCCGCCT

GTCCAAGTTCCCCTACGAGCTGCGCCAGGAGATCGCCCCCCACTTCGTGGACTCCGACCCC

GTGATCGAGGACAACCGCCGCCTGCTGAACTTCGACGTGAAGACCGGCGACTCCATCCGC

AAGGGCCTGACCCCCCGCTGGAACGACCTGGACGTGAACCAGCACGTGAACAACGTGAA

GTACATCGGCTGGATCCTGGAGTCCGTGCCCACCGAGGTGTTCGACACCCGCGAGCTGTG

CGGCCTGACCCTGGAGTACCGCCAGGAGTGCGGCCGCGGCTCCGTGCTGGAGTCCGTGAC

CGCCATGGACCCCTCC AAGGAGGGCGACCGCTCCCT GT ACC AGCACCTGCT GCGCCT GGA

GGACGGCACCGACATCGTGAAGGGCCGCACCGAGTGGCGCCCCAAGAACGCCGGCACCA

ACGGCCCCGTGTCCACCCGCAAGACCACCAACGGCTCCTCCGTGTCCTGA SEQ ID NO: 82

Cuphea painteri (Cpai) FATB1 amino acid sequence

MVAAAATSAFFPVPAPGTSPNPRKFGSWPSSLSPSLPKSIPNGGFQVKANASAHPKANGSAVSL

KSGSLNTQENTSSSPPPRTFLHQLPDWSRLLTAITTVFVKSKRPDMHDRKSKRPDMLVDLFGLE

SSVQDALVFRQSFSIRSYEIGTDRTASIETLMNHLQETSLNHCKSTGILLDGFGRTLEMCKRELI

WWIKMQIQVNRYPAWGDTVEINTRFSRLGKIGMGRDWLISDCNTGEILIRATSEYAMMNQK

TRRLSKLPYEVHQEIAPLFVDSPPVIEDNDLKVHKFEVKTGDSIQKGLSPGWNDLDVNQHVSN

VKYIGWILESMPTEVLETQELCSLALEYRRECGRDSVLESVTAMDPSKVGGRSQYQHLLRLED

GTAIVNGITEWRPKNAGANGAISTGKTSNGNSVS SEQ ID NO: 83

Cuphea painteri (Cpai) FATB1 coding DNA sequence

ATGGTGGCTGCTGCAGCAACTTCTGCATTCTTCCCTGTTCCAGCCCCGGGAACCTCCCCAA

ATCCCAGGAAATTCGGAAGTTGGCCATCGAGCTTGAGCCCTTCCTTGCCCAAGTCAATCCC

CAATGGCGGATTTCAGGTAAAGGCAAATGCCAGTGCCCATCCGAAGGCTAACGGTTCTGC

AGTTAGTCTAAAGTCTGGCAGCCTCAACACTCAGGAGAACACTTCGTCGTCCCCTCCTCCT

CGGACTTTCCTTCACCAGTTGCCTGATTGGAGTAGGCTTCTGACTGCAATCACGACCGTGT

TCGTGAAATCTAAGAGGCCTGACATGCATGATCGGAAATCTAAGAGGCCTGACATGCTGG

TGGACTTGTTTGGGTTGGAAAGTAGTGTTCAGGATGCGCTCGTGTTCAGACAGAGTTTTTC

GATTAGGTCTTATGAAATAGGCACTGATCGAACAGCCTCTATAGAGACGCTGATGAACCA

CTTGCAGGAAACATCTCTCAATCATTGTAAAAGTACCGGTATTCTCCTTGACGGCTTCGGT

CGTACTCTTGAGATGTGTAAAAGGGAACTCATTTGGGTGGTAATAAAAATGCAAATTCAG

GTGAATCGCTATCCAGCATGGGGCGATACTGTCGAGATCAATACCCGGTTCTCCCGGTTGG

GGAAAATTGGTATGGGTCGCGATTGGCTAATAAGTGATTGCAACACAGGAGAAATTCTAA

TAAGAGCAACGAGCGAGTATGCCATGATGAATCAAAAGACGAGAAGACTCTCAAAACTT

CCATACGAGGTTCACCAGGAGATAGCGCCTCTTTTTGTCGACTCTCCTCCTGTGATTGAAG

ACAATGATCTGAAAGTGCATAAATTTGAAGTGAAGACTGGTGATTCCATTCAAAAGGGTC

TATCCCCGGGGTGGAATGACTTGGATGTCAATCAGCACGTAAGCAACGTGAAGTACATTG

GGTGGATTCTCGAGAGTATGCCAACAGAAGTTTTGGAGACCCAGGAGCTATGCTCTCTCG

CCCTTGAATATAGGCGGGAATGCGGAAGGGACAGTGTGCTGGAGTCCGTGACCGCAATGG

ATCCCTCAAAAGTTGGAGGCCGTTCTCAGTACCAGCACCTTCTGCGGCTTGAGGATGGGA

CTGCTATCGTGAACGGCATAACTGAGTGGCGGCCGAAGAATGCAGGAGCTAATGGGGCG

ATATCAACGGGAAAGACTTCAAATGGAAACTCGGTCTCTTAG SEQ ID NO: 84

Cuphea painteri (Cpai) FATB1 coding DNA sequence codon optimized for Prototheca moriformis

ATGGTGGCCGCCGCCGCCACCTCCGCCTTCTTCCCCGTGCCCGCCCCCGGCACCTCCCCCA

ACCCCCGCAAGTTCGGCTCCTGGCCCTCCTCCCTGTCCCCCTCCCTGCCCAAGTCCATCCCC

AACGGCGGCTTCCAGGTGAAGGCCAACGCCTCCGCCCACCCCAAGGCCAACGGCTCCGCC

GTGTCCCTGAAGTCCGGCTCCCTGAACACCCAGGAGAACACCTCCTCCTCCCCCCCCCCCC

GCACCTTCCTGCACCAGCTGCCCGACTGGTCCCGCCTGCTGACCGCCATCACCACCGTGTT

CGTGAAGTCCAAGCGCCCCGACATGCACGACCGCAAGTCCAAGCGCCCCGACATGCTGGT

GGACCTGTTCGGCCTGGAGTCCTCCGTGCAGGACGCCCTGGTGTTCCGCCAGTCCTTCTCC

ATCCGCTCCTACGAGATCGGCACCGACCGCACCGCCTCCATCGAGACCCTGATGAACCAC

CTGCAGGAGACCTCCCTGAACCACTGCAAGTCCACCGGCATCCTGCTGGACGGCTTCGGC

CGCACCCTGGAGATGTGCAAGCGCGAGCTGATCTGGGTGGTGATCAAGATGCAGATCCAG

GTGAACCGCTACCCCGCCTGGGGCGACACCGTGGAGATCAACACCCGCTTCTCCCGCCTG

GGCAAGATCGGCATGGGCCGCGACTGGCTGATCTCCGACTGCAACACCGGCGAGATCCTG

ATCCGCGCCACCTCCGAGTACGCCATGATGAACCAGAAGACCCGCCGCCTGTCCAAGCTG

CCCTACGAGGTGCACCAGGAGATCGCCCCCCTGTTCGTGGACTCCCCCCCCGTGATCGAG

GACAACGACCTGAAGGTGCACAAGTTCGAGGTGAAGACCGGCGACTCCATCCAGAAGGG

CCTGTCCCCCGGCTGGAACGACCTGGACGTGAACCAGCACGTGTCCAACGTGAAGTACAT

CGGCTGGATCCTGGAGTCCATGCCCACCGAGGTGCTGGAGACCCAGGAGCTGTGCTCCCT

GGCCCTGGAGTACCGCCGCGAGTGCGGCCGCGACTCCGTGCTGGAGTCCGTGACCGCCAT

GGACCCCTCCAAGGTGGGCGGCCGCTCCCAGTACCAGCACCTGCTGCGCCTGGAGGACGG

CACCGCCATCGTGAACGGCATCACCGAGTGGCGCCCCAAGAACGCCGGCGCCAACGGCGC

CATCTCCACCGGCAAGACCTCCAACGGCAACTCCGTGTCCTGA SEQ ID NO: 85

Cuphea hookeriana (Chook) FATB4 amino acid sequence

MVAAAATSAFFPVPAPGTSPNPRKFGSWPSSLSPSLPNSIPNGGFQVKANASAHPKANGSAVSL KSGSLNTQENT S S SPPPRTFLHQLPDW SRLLT AITTVFVKSKRPDMHDRKSKRPDMLVDLFGLE SSVQDALVFRQRFSIRSYEIGTDRTASMETLMNHLQETSLNHCKSTGILLDGFGRTLEMCKREL IWWIKMQIQVNRYPAWGDTVEINTRFSRLGKIGMGRDWLISDCNTGEILIRATSEYAMMNQK TRRLSKLPYEVRQEIAPLFVDSPPVIEDNDLKVHKFEVKTGDSIHKGLTPGWNDLDVNQHVNN VKYIGWILESTPPEVLETQELCSLTLEYRRECGRESVLESLTAMDPSGGGYGSQFQHLLRLEDG GEIVKGRTEWRPKNGVINGWPTGESSPGDYS SEQ ID NO: 86

Cuphea hookeriana (Chook) FATB4 coding DNA sequence

ATGGTGGCTGCTGCAGCAACTTCTGCATTCTTCCCTGTTCCAGCCCCGGGAACCTCCCCTA

ATCCCAGGAAATTCGGAAGTTGGCCATCGAGCTTGAGCCCTTCCTTGCCCAACTCAATCCC

CAATGGCGGATTTCAGGTAAAGGCAAATGCCAGTGCCCATCCGAAGGCTAACGGTTCTGC

AGTTAGTCTAAAGTCTGGCAGCCTCAACACTCAGGAGAACACTTCGTCGTCCCCTCCTCCT

CGGACTTTCCTTCACCAGTTGCCTGATTGGAGTAGGCTTCTGACTGCAATCACGACCGTGT

TCGTGAAATCTAAGAGGCCTGACATGCATGATCGGAAATCTAAGAGGCCTGACATGCTGG

TGGACTTGTTTGGGTTGGAGAGTAGTGTTCAGGATGCGCTCGTGTTCAGACAGAGATTTTC

GATTAGGTCTTATGAAATAGGCACTGATCGAACAGCCTCTATGGAGACGCTGATGAACCA

CTTGCAGGAAACATCTCTCAATCATTGTAAAAGTACCGGTATTCTCCTTGACGGCTTCGGT

CGTACTCTTGAGATGTGTAAAAGGGAACTCATTTGGGTGGTAATAAAAATGCAGATTCAG

GTGAATCGCTATCCAGCATGGGGCGATACTGTCGAGATCAATACCCGGTTCTCCCGGTTGG

GGAAAATTGGTATGGGTCGCGATTGGCTAATAAGTGATTGCAACACAGGAGAAATTCTTA

TAAGAGCAACGAGCGAGTATGCCATGATGAATCAAAAGACGAGAAGACTCTCAAAACTT

CCATACGAGGTTCGCCAGGAGATAGCGCCTCTTTTTGTCGACTCTCCTCCTGTGATTGAAG

ACAATGATCTGAAAGTGCATAAATTTGAAGTGAAGACTGGTGATTCCATTCACAAGGGTC

TAACTCCGGGGTGGAATGACTTGGATGTCAATCAGCACGTCAACAACGTGAAGTACATCG

GGTGGATTCTTGAGAGTACTCCACCAGAAGTTCTGGAGACCCAGGAGTTATGTTCCCTTAC

TCTGGAATACAGGCGGGAATGTGGAAGGGAGAGCGTGCTGGAGTCCCTCACTGCTATGGA

TCCCTCTGGAGGGGGTTATGGGTCCCAGTTTCAGCACCTTCTGCGGCTTGAGGATGGAGGT

GAGATCGTGAAGGGGAGAACCGAGTGGCGACCCAAGAATGGTGTAATCAATGGGGTGGT

ACCAACCGGGGAGTCCTCACCTGGAGACTACTCTTAG SEQ ID NO: 87

Cuphea hookeriana (Chook) FATB4 coding DNA sequence codon optimized for Prototheca moriformis

ATGGTGGCCGCCGCCGCCACCTCCGCCTTCTTCCCCGTGCCCGCCCCCGGCACCTCCCCCA

ACCCCCGCAAGTTCGGCTCCTGGCCCTCCTCCCTGTCCCCCTCCCTGCCCAACTCCATCCCC

AACGGCGGCTTCCAGGTGAAGGCCAACGCCTCCGCCCACCCCAAGGCCAACGGCTCCGCC

GTGTCCCTGAAGTCCGGCTCCCTGAACACCCAGGAGAACACCTCCTCCTCCCCCCCCCCCC

GCACCTTCCTGCACCAGCTGCCCGACTGGTCCCGCCTGCTGACCGCCATCACCACCGTGTT

CGTGAAGTCCAAGCGCCCCGACATGCACGACCGCAAGTCCAAGCGCCCCGACATGCTGGT

GGACCTGTTCGGCCTGGAGTCCTCCGTGCAGGACGCCCTGGTGTTCCGCCAGCGCTTCTCC

ATCCGCTCCTACGAGATCGGCACCGACCGCACCGCCTCCATGGAGACCCTGATGAACCAC

CTGCAGGAGACCTCCCTGAACCACTGCAAGTCCACCGGCATCCTGCTGGACGGCTTCGGC

CGCACCCTGGAGATGTGCAAGCGCGAGCTGATCTGGGTGGTGATCAAGATGCAGATCCAG

GTGAACCGCTACCCCGCCTGGGGCGACACCGTGGAGATCAACACCCGCTTCTCCCGCCTG

GGCAAGATCGGCATGGGCCGCGACTGGCTGATCTCCGACTGCAACACCGGCGAGATCCTG

ATCCGCGCCACCTCCGAGTACGCCATGATGAACCAGAAGACCCGCCGCCTGTCCAAGCTG

CCCTACGAGGTGCGCCAGGAGATCGCCCCCCTGTTCGTGGACTCCCCCCCCGTGATCGAG

GACAACGACCTGAAGGTGCACAAGTTCGAGGTGAAGACCGGCGACTCCATCCACAAGGG

CCTGACCCCCGGCTGGAACGACCTGGACGTGAACCAGCACGTGAACAACGTGAAGTACAT

CGGCTGGATCCTGGAGTCCACCCCCCCCGAGGTGCTGGAGACCCAGGAGCTGTGCTCCCT

GACCCTGGAGTACCGCCGCGAGTGCGGCCGCGAGTCCGTGCTGGAGTCCCTGACCGCCAT

GGACCCCTCCGGCGGCGGCTACGGCTCCCAGTTCCAGCACCTGCTGCGCCTGGAGGACGG

CGGCGAGATCGTGAAGGGCCGCACCGAGTGGCGCCCCAAGAACGGCGTGATCAACGGCG

TGGTGCCCACCGGCGAGTCCTCCCCCGGCGACTACTCCTGA SEQ ID NO: 88

Cuphea avigera var. pulcherrima (Ca) FATB1 amino acid sequence

MVAAAASSAFFSVPVPGTSPKPGKFRIWPSSLSPSFKPKPIPNGGLQVKANSRAHPKANGSAVS

LKSGSLNTQEDTSSSPPPRTFLHQLPDWSRLLTAITTVFVKSKRPDMHDRKSKRPDMLMDSFG

LESIVQEGLEFRQSFSIRSYEIGTDRTASIETLMNYLQETSLNHCKSTGILLDGFGRTPEMCKRDL

IWWTKMKIKVNRYPAWGDTVEINTWFSRLGKIGKGRDWLISDCNTGEILIRATSAYATMNQ

KTRRLSKLPYEVHQEIAPLFVDSPPVIEDNDLKLHKFEVKTGDSIHKGLTPGWNDLDVNQHVS

NVKYIGWILESMPTEVLETQELCSLALEYRRECGRDSVLESVTAMDPTKVGGRSQYQHLLRLE

DGTDIVKCRTEWRPKNPGANGAISTGKTSNGNSVS SEQ ID NO: 89

Cuphea avigera var. pulcherrima (Ca) FATB1 coding DNA sequence

ATGGTGGCTGCTGCAGCAAGTTCTGCATTCTTCTCTGTTCCAGTCCCGGGAACCTCTCCTA

AACCCGGGAAGTTCAGAATTTGGCCATCGAGCTTGAGCCCTTCCTTCAAGCCCAAGCCGA

TCCCCAATGGTGGATTGCAGGTTAAGGCAAATTCCAGGGCACATCCGAAGGCTAACGGTT

CTGCAGTTAGTCTAAAGTCTGGCAGCCTCAACACTCAGGAGGACACTTCGTCGTCCCCTCC

TCCTCGGACTTTCCTTCACCAGTTGCCTGATTGGAGTAGGCTTCTGACTGCAATCACGACC

GTGTTCGTGAAATCTAAGAGGCCTGACATGCATGATCGGAAATCTAAGAGGCCTGACATG

CTGATGGACTCGTTTGGGTTGGAGAGTATTGTTCAAGAAGGGCTCGAGTTCAGACAGAGT

TTTTCGATTAGGTCTTATGAAATAGGCACTGATCGAACAGCCTCTATAGAGACGCTGATGA

ACTACTTGCAGGAAACATCTCTCAATCATTGTAAGAGTACCGGTATTCTCCTTGACGGCTT

TGGTCGTACTCCTGAGATGTGTAAAAGGGACCTCATTTGGGTGGTAACAAAAATGAAGAT

CAAGGTGAATCGCTATCCAGCTTGGGGCGATACTGTCGAGATCAATACCTGGTTCTCCCGG

TTGGGGAAAATCGGAAAGGGTCGCGATTGGCTAATAAGTGATTGCAACACAGGAGAAATT

CTTATAAGAGCAACGAGCGCGTATGCCACGATGAATCAAAAGACGAGAAGACTCTCAAA

ACTTCCATACGAGGTTCACCAGGAGATAGCGCCTCTCTTTGTCGACTCTCCTCCTGTCATT

GAAGACAATGATCTGAAATTGCATAAGTTTGAAGTGAAGACTGGTGATTCCATTCACAAG

GGTCTAACTCCGGGGTGGAATGACTTGGATGTCAATCAGCACGTAAGCAACGTGAAGTAC

ATTGGGTGGATTCTCGAGAGTATGCCAACAGAAGTTTTGGAGACCCAGGAGCTATGCTCT

CTCGCCCTTGAATATAGGCGGGAATGCGGAAGGGACAGTGTGCTAGAGTCCGTGACAGCT

ATGGATCCCACAAAAGTTGGAGGCCGGTCTCAGTACCAGCACCTTCTGCGACTTGAGGAT

GGGACTGATATCGTGAAGTGCAGAACTGAGTGGCGGCCGAAGAATCCAGGAGCTAATGG

GGC AAT ATCAACGGGAAAGACTTC AAATGGAAACTCGGTCTCTT AG SEQ ID NO: 90

Cuphea avigera var. pulcherrima (Ca) FATB1 coding DNA sequence codon optimized for Prototheca moriformis

AT GGT GGCCGCCGCCGCCTCCT CCGCCTT CTT CT CCGT GCCCGT GCCCGGC ACCTCCCCC A

AGCCCGGCAAGTTCCGCATCTGGCCCTCCTCCCTGTCCCCCTCCTTCAAGCCCAAGCCCAT

CCCCAACGGCGGCCTGCAGGTGAAGGCCAACTCCCGCGCCCACCCCAAGGCCAACGGCTC

CGCCGTGTCCCTGAAGTCCGGCTCCCTGAACACCCAGGAGGACACCTCCTCCTCCCCCCCC

CCCCGC ACCTT CCT GC ACC AGCT GCCCGACT GGTCCCGCCT GCT G ACCGCC ATC ACC ACCG

TGTTCGTGAAGTCCAAGCGCCCCGACATGCACGACCGCAAGTCCAAGCGCCCCGACATGC

TGATGGACTCCTTCGGCCTGGAGTCCATCGTGCAGGAGGGCCTGGAGTTCCGCCAGTCCTT

CTCCATCCGCTCCTACGAGATCGGCACCGACCGCACCGCCTCCATCGAGACCCTGATGAA

CTACCTGCAGGAGACCTCCCTGAACCACTGCAAGTCCACCGGCATCCTGCTGGACGGCTTC

GGCCGCACCCCCGAGATGTGCAAGCGCGACCTGATCTGGGTGGTGACCAAGATGAAGATC

AAGGTGAACCGCTACCCCGCCTGGGGCGACACCGTGGAGATCAACACCTGGTTCTCCCGC

CTGGGCAAGATCGGCAAGGGCCGCGACTGGCTGATCTCCGACTGCAACACCGGCGAGATC

CTGATCCGCGCCACCTCCGCCTACGCCACCATGAACCAGAAGACCCGCCGCCTGTCCAAG

CTGCCCTACGAGGTGCACCAGGAGATCGCCCCCCTGTTCGTGGACTCCCCCCCCGTGATCG

AGGACAACGACCTGAAGCTGCACAAGTTCGAGGTGAAGACCGGCGACTCCATCCACAAG

GGCCTGACCCCCGGCTGGAACGACCTGGACGTGAACCAGCACGTGTCCAACGTGAAGTAC

ATCGGCTGGATCCTGGAGTCCATGCCCACCGAGGTGCTGGAGACCCAGGAGCTGTGCTCC

CTGGCCCTGGAGTACCGCCGCGAGTGCGGCCGCGACTCCGTGCTGGAGTCCGTGACCGCC

AT GGACCCC ACC AAGGT GGGCGGCCGCT CCC AGT ACCAGCACCTGCT GCGCCTGGAGGAC

GGCACCGACATCGTGAAGTGCCGCACCGAGTGGCGCCCCAAGAACCCCGGCGCCAACGG

CGCCATCTCCACCGGCAAGACCTCCAACGGCAACTCCGTGTCC SEQ ID NO: 91

Cuphea paucipetala (Cpau) FATB1 amino acid sequence

MVAAAASSAFFPVPAPGTSPKPGKSGNWPSSLSPSIKPMSIPNGGFQVKANASAHPKANGSAV

NLKSGSLNTQEDTSSSPPPRAFLNQLPDWSMLLTAITTVFVAAEKQWTMRDRKSKRPDMLVD

SVGLKSWLDGLVSRQIFSIRSYEIGADRTASIETLMNHLQETSINHCKSLGLLNDGFGRTPGMC

KNDLIWVLTKMQIMVNRYPTWGDTVEINTWFSHSGKIGMASDWLITDCNTGEILIRATSVWA

MMNQKTRRFSRLPYEVRQELTPHYVDSPHVIEDNDRKLHKFDVKTGDSIRKGLTPRWNDLDV

NQHVSNVKYIGWILESMPIEVLETQELCSLTVEYRRECGMDSVLESVTAMDPSEDEGRSQYKH

LLRLEDGTDIVKGRTEWRPKNAGTNGAISTAKPSNGNSVS SEQ ID NO: 92

Cuphea paucipetala (Cpau) FATB1 coding DNA sequence

ATGGTGGCTGCTGCAGCAAGTTCTGCATTCTTCCCTGTTCCAGCCCCCGGAACCTCCCCTA

AACCCGGGAAGTCCGGCAACTGGCCATCAAGCTTGAGCCCTTCCATCAAGCCCATGTCAA

TCCCCAATGGCGGATTTCAGGTTAAGGCAAATGCCAGTGCCCATCCTAAGGCTAACGGTT

CTGCAGTAAATCTAAAGTCTGGCAGCCTCAACACTCAGGAGGACACTTCGTCGTCCCCTCC

TCCTCGGGCTTTCCTTAACC AGTTGCCT GATTGGAGTAT GCTT CT GACTGC AAT CACGACC

GTCTTCGTGGCGGCAGAGAAGCAGTGGACTATGCGTGATCGGAAATCTAAGAGGCCTGAC

ATGCTCGTGGACTCGGTTGGGTTGAAGAGTGTTGTTCTGGATGGGCTCGTGTCCAGACAGA

TTTTTTCGATTAGGTCTTATGAAATAGGCGCTGATCGAACTGCCTCTATAGAGACGCTGAT

GAACCACTTGCAGGAAACATCTATCAATCATTGTAAGAGTTTGGGTCTTCTCAATGACGGC

TTTGGTCGTACTCCTGGGATGTGTAAAAATGACCTCATTTGGGTGCTTACAAAAATGCAGA

TCATGGTGAATCGCTACCCAACTTGGGGCGATACTGTTGAGATCAATACCTGGTTCTCCCA

TTCGGGGAAAATTGGTATGGCTAGCGATTGGCTAATAACTGATTGCAACACAGGAGAAAT

TCTTATAAGAGCAACGAGCGTGTGGGCCATGATGAATCAAAAGACGAGAAGATTCTCAAG

ACTTCCATACGAGGTTCGCCAGGAGTTAACGCCTCATTATGTGGACTCTCCTCATGTCATT

GAAGATAATGATCGGAAATTGCATAAGTTTGATGTGAAGACTGGTGATTCCATTCGTAAG

GGTCTAACTCCGAGGTGGAATGACTTGGATGTCAATCAGCACGTAAGCAACGTGAAGTAC

ATTGGGTGGATTCTCGAGAGTATGCCAATAGAAGTTTTGGAGACCCAGGAGCTATGCTCT

CTCACCGTTGAATATAGGCGGGAATGCGGAATGGACAGTGTGCTGGAGTCCGTGACTGCT

ATGGATCCCTCAGAAGATGAAGGCCGGTCTCAGTACAAGCACCTTCTGCGGCTTGAGGAT

GGGACTGACATCGTGAAGGGCAGAACTGAGTGGCGACCGAAGAATGCAGGAACTAACGG

GGCGATATCAACAGCAAAGCCTTCAAATGGAAACTCGGTCTCTTAG SEQ ID NO: 93

Cuphea paucipetala (Cpau) FATB1 coding DNA sequence codon optimized for Pro to theca moriformis

ATGGTGGCCGCCGCCGCCTCCTCCGCCTTCTTCCCCGTGCCCGCCCCCGGCACCTCCCCCA

AGCCCGGCAAGTCCGGCAACTGGCCCTCCTCCCTGTCCCCCTCCATCAAGCCCATGTCCAT

CCCCAACGGCGGCTTCCAGGTGAAGGCCAACGCCTCCGCCCACCCCAAGGCCAACGGCTC

CGCCGTGAACCTGAAGTCCGGCTCCCTGAACACCCAGGAGGACACCTCCTCCTCCCCCCCC

CCCCGCGCCTTCCTGAACCAGCTGCCCGACTGGTCCATGCTGCTGACCGCCATCACCACCG

TGTTCGTGGCCGCCGAGAAGCAGTGGACCATGCGCGACCGCAAGTCCAAGCGCCCCGACA

TGCTGGTGGACTCCGTGGGCCTGAAGTCCGTGGTGCTGGACGGCCTGGTGTCCCGCCAGA

TCTTCTCCATCCGCTCCTACGAGATCGGCGCCGACCGCACCGCCTCCATCGAGACCCTGAT

GAACC ACCT GC AGGAGACCT CC AT CAACC ACT GCAAGTCCCTGGGCCTGCT GAACGACGG

CTTCGGCCGCACCCCCGGCATGTGCAAGAACGACCTGATCTGGGTGCTGACCAAGATGCA

GAT CAT GGT GAACCGCT ACCCC ACCTGGGGCGAC ACCGTGG AGATC AAC ACCT GGTTCTC

CCACTCCGGCAAGATCGGCATGGCCTCCGACTGGCTGATCACCGACTGCAACACCGGCGA

GATCCTGATCCGCGCCACCTCCGTGTGGGCCATGATGAACCAGAAGACCCGCCGCTTCTCC

CGCCTGCCCTACGAGGTGCGCCAGGAGCTGACCCCCCACTACGTGGACTCCCCCCACGTG

AT CGAGGAC AACGACCGC AAGCT GCACAAGTT CGACGT GAAGACCGGCGACTCC ATCCGC

AAGGGCCTGACCCCCCGCTGGAACGACCTGGACGTGAACCAGCACGTGTCCAACGTGAAG

TACATCGGCTGGATCCTGGAGTCCATGCCCATCGAGGTGCTGGAGACCCAGGAGCTGTGC

TCCCTGACCGTGGAGTACCGCCGCGAGTGCGGCATGGACTCCGTGCTGGAGTCCGTGACC

GCCATGGACCCCTCCGAGGACGAGGGCCGCTCCCAGTACAAGCACCTGCTGCGCCTGGAG

GACGGCACCGACATCGTGAAGGGCCGCACCGAGTGGCGCCCCAAGAACGCCGGCACCAA

CGGCGCCATCTCCACCGCCAAGCCCTCCAACGGCAACTCCGTGTCCTGA SEQ ID NO: 94

Cuphea procumbens (Cproc) FATB1 amino acid sequence

MVAAAASSAFFPAPAPGSSPKPGKSGNWPSSLSPSFKSKSIPYGRFQVKANASAHPKANGSAV

NLKSGSLNTQEDTSSSPPPRAFLNQLPDWSMLLSAITTVFVAAEKQWTMLDRKSKRPDMLVD

SVGLKNIVRDGLVSRQSFLIRSYEIGADRTASIETLMNHLQETSINHCKSLGLLNDGFGRTPGM

CKNDLIWVLTKMQIMVNRYPAWGDTVEINTWFSQSGKIGMGSDWLISDCNTGEILIRATSVW

AMMNQKTRRFSRLPYEVRQELTPHFVDSPHVIEDNDRKLHKFDVKTGDSIRKGLTPRWNDLD

VNQHVSNVKYIGWILESMPIEVLEAQELCSLTVEYRRECGMDSVLESVTAVDPSEDGGRSQYN

HLLRLEDGTDWKGRTEWRPKNAETNGAISPGNTSNGNSIS SEQ ID NO: 95

Cuphea procumbens (Cproc) FATB1 coding DNA sequence

ATGGTGGCTGCTGCAGCAAGTTCTGCATTCTTCCCTGCTCCAGCCCCGGGATCCTCACCTA

AACCCGGGAAGTCCGGTAATTGGCCATCGAGCTTGAGCCCTTCCTTCAAGTCCAAGTCAAT

CCCCTATGGCCGATTTCAGGTTAAGGCAAATGCCAGTGCCCATCCTAAGGCTAACGGTTCT

GC AGT AAAT CT AAAGT CT GGC AGCCTC AAC ACT C AGGAGGAC ACTT CGTCGT CCCCTCCTC

CTCGGGCTTTCCTTAACCAGTTGCCTGATTGGAGTATGCTTCTGTCTGCAATCACGACTGT

ATTCGTGGCGGCAGAGAAGCAGTGGACTATGCTTGATCGGAAATCTAAGAGGCCTGACAT

GCTTGTGGACT CGGTT GGGTT GAAGAAT ATT GTT CGGGAT GGGCT CGT GT CC AGAC AGAG

TTTTTTGATTAGATCTTATGAAATAGGCGCTGATCGAACAGCTTCTATAGAGACACTGATG

AACCACTTGCAGGAAACATCTATCAATCATTGTAAGAGTTTGGGTCTTCTCAATGACGGCT

TTGGTCGTACTCCTGGGATGTGTAAAAACGACCTCATTTGGGTGCTTACTAAAATGCAGAT

CATGGTGAATCGCTACCCAGCTTGGGGCGATACTGTTGAGATCAATACCTGGTTCTCCCAG

TCGGGGAAAATCGGTATGGGTAGCGATTGGCTAATAAGTGATTGCAACACAGGAGAAATT

CTTATAAGAGCAACGAGCGTGTGGGCCATGATGAATCAAAAAACGAGAAGATTCTCAAG

ACTTCCATACGAGGTTCGCCAGGAGTTAACGCCTCATTTTGTGGACTCTCCTCATGTCATT

GAAGACAATGATCGGAAATTGCATAAGTTCGATGTGAAGACTGGTGATTCTATTCGCAAG

GGTCTAACTCCGAGGTGGAATGACTTGGATGTCAATCAGCACGTGAGCAACGTGAAGTAC

ATTGGGTGGATTCTCGAGAGTATGCCAATAGAAGTTTTGGAGGCCCAGGAACTATGCTCT

CTCACCGTTGAATATAGGCGGGAATGCGGAATGGACAGTGTGCTGGAGTCCGTGACTGCT

GTAGATCCCTCAGAAGATGGAGGCCGGTCTCAGTACAATCACCTTCTGCGGCTTGAGGAT

GGGACTGATGTCGTGAAGGGCAGAACTGAGTGGCGACCGAAGAATGCAGAAACTAACGG

GGCGATATCACCAGGAAACACTTCAAATGGAAACTCGATCTCCTAG SEQ ID NO: 96

Cuphea procumbens (Cproc) FATB1 coding DNA sequence codon optimized for Prototheca moriformis

ATGGTGGCCGCCGCCGCCTCCTCCGCCTTCTTCCCCGCCCCCGCCCCCGGCTCCTCCCCCA

AGCCCGGCAAGTCCGGCAACTGGCCCTCCTCCCTGTCCCCCTCCTTCAAGTCCAAGTCCAT

CCCCTACGGCCGCTTCCAGGTGAAGGCCAACGCCTCCGCCCACCCCAAGGCCAACGGCTC

CGCCGTGAACCTGAAGTCCGGCTCCCTGAACACCCAGGAGGACACCTCCTCCTCCCCCCCC

CCCCGCGCCTTCCTGAACCAGCTGCCCGACTGGTCCATGCTGCTGTCCGCCATCACCACCG

TGTTCGTGGCCGCCGAGAAGCAGTGGACCATGCTGGACCGCAAGTCCAAGCGCCCCGACA

TGCTGGTGGACTCCGTGGGCCTGAAGAACATCGTGCGCGACGGCCTGGTGTCCCGCCAGT

CCTTCCTGATCCGCTCCTACGAGATCGGCGCCGACCGCACCGCCTCCATCGAGACCCTGAT

GAACCACCTGCAGGAGACCTCCATCAACCACTGCAAGTCCCTGGGCCTGCTGAACGACGG

CTTCGGCCGCACCCCCGGCATGTGCAAGAACGACCTGATCTGGGTGCTGACCAAGATGCA

GATCATGGTGAACCGCTACCCCGCCTGGGGCGACACCGTGGAGATCAACACCTGGTTCTC

CCAGTCCGGCAAGATCGGCATGGGCTCCGACTGGCTGATCTCCGACTGCAACACCGGCGA

GATCCTGATCCGCGCCACCTCCGTGTGGGCCATGATGAACCAGAAGACCCGCCGCTTCTCC

CGCCTGCCCTACGAGGTGCGCCAGGAGCTGACCCCCCACTTCGTGGACTCCCCCCACGTG

ATCGAGGACAACGACCGCAAGCTGCACAAGTTCGACGTGAAGACCGGCGACTCCATCCGC

AAGGGCCTGACCCCCCGCTGGAACGACCTGGACGTGAACCAGCACGTGTCCAACGTGAAG

TACATCGGCTGGATCCTGGAGTCCATGCCCATCGAGGTGCTGGAGGCCCAGGAGCTGTGC

TCCCTGACCGTGGAGTACCGCCGCGAGTGCGGCATGGACTCCGTGCTGGAGTCCGTGACC

GCCGTGGACCCCTCCGAGGACGGCGGCCGCTCCCAGTACAACCACCTGCTGCGCCTGGAG

GACGGCACCGACGTGGTGAAGGGCCGCACCGAGTGGCGCCCCAAGAACGCCGAGACCAA

CGGCGCCATCTCCCCCGGCAACACCTCCAACGGCAACTCCATCTCCTGA SEQ ID NO: 97

Cuphea procumbens (Cproc) FATB2 amino acid sequence

MVAAAASSAFFPAPAPGSSPKPGKSGNWPSSLSPSFKSKSIPYGRFQVKANASAHPKANGSAV

NLKSGSLNTQEDTSSSPPPRAFLNQLPDWSMLLSAITTVFVAAEKQWTMLDRKSKRPDMLVD

SVGLKNIVRDGLVSRQSFLIRSYEIGADRTASIETLMNHLQETSINHCKSLGLLNDGFGRTPGM

CKNDLIWVLTKMQIMVNRYPAWGDTVEINTWFSQSGKIGMGSDWLISDCNTGEILIRATSVW

AMMNQKTRRFSRLPYEVRQELTPHFVDSPHVIEDNDRKLHKFDVKTGDSIRKGLTPRWNDLD

VNQHVNNVKYIGWILESTPPEVLETQELCSLTLEYRQECGRESVLESLTAVDPSGKGFGSQFQH

LLRLEDGGEIVKGRTEWRPKTAGINGAIASGETSPGDF SEQ ID NO: 98

Cuphea procumbens (Cproc) FATB2 coding DNA sequence

ATGGTGGCTGCTGCAGCAAGTTCTGCATTCTTCCCTGCTCCAGCCCCGGGATCCTCACCTA

AACCCGGGAAGTCCGGTAATTGGCCATCGAGCTTGAGCCCTTCCTTCAAGTCCAAGTCAAT

CCCCTATGGCCGATTTCAGGTTAAGGCAAATGCCAGTGCCCATCCTAAGGCTAACGGTTCT

GCAGTAAATCTAAAGTCTGGCAGCCTCAACACTCAGGAGGACACTTCGTCGTCCCCTCCTC

CTCGGGCTTTCCTTAACCAGTTGCCTGATTGGAGTATGCTTCTGTCTGCAATCACGACTGT

ATTCGTGGCGGCAGAGAAGCAGTGGACTATGCTTGATCGGAAATCTAAGAGGCCTGACAT

GCTTGTGGACTCGGTTGGGTTGAAGAATATTGTTCGGGATGGGCTCGTGTCCAGACAGAG

TTTTTTGATTAGATCTTATGAAATAGGCGCTGATCGAACAGCTTCTATAGAGACACTGATG

AACCACTTGCAGGAAACATCTATCAATCATTGTAAGAGTTTGGGTCTTCTCAATGACGGCT

TTGGTCGTACTCCTGGGATGTGTAAAAACGACCTCATTTGGGTGCTTACTAAAATGCAGAT

CATGGTGAATCGCTACCCAGCTTGGGGCGATACTGTTGAGATCAATACCTGGTTCTCCCAG

TCGGGGAAAATCGGTATGGGTAGCGATTGGCTAATAAGTGATTGCAACACAGGAGAAATT

CTTATAAGAGCAACGAGCGTGTGGGCCATGATGAATCAAAAAACGAGAAGATTCTCAAG

ACTTCCATACGAGGTTCGCCAGGAGTTAACGCCTCATTTTGTGGACTCTCCTCATGTCATT

GAAGACAATGATCGGAAATTGCATAAGTTCGATGTGAAGACTGGTGATTCTATTCGCAAG

GGTCTAACTCCGAGGTGGAATGACTTGGATGTCAATCAGCACGTCAACAACGTGAAGTAC

ATCGGGTGGATTCTTGAGAGTACTCCACCAGAAGTTCTGGAGACCCAGGAGTTATGTTCCC

TTACCCTGGAATACAGGCAGGAATGCGGAAGGGAGAGCGTGCTGGAGTCCCTCACTGCTG

TGGACCCCTCTGGAAAGGGCTTTGGGTCCCAGTTCCAACACCTTCTGAGGCTTGAGGATGG

AGGTGAGATCGTGAAGGGGAGAACTGAGTGGCGACCCAAGACTGCAGGTATCAATGGGG

CGATAGCATCCGGGGAGACCTCACCTGGAGACTTTTAG SEQ ID NO: 99

Cuphea procumbens (Cproc) FATB2 coding DNA sequence codon optimized for Prototheca moriformis

ATGGTGGCCGCCGCCGCCTCCTCCGCCTTCTTCCCCGCCCCCGCCCCCGGCTCCTCCCCCA

AGCCCGGCAAGTCCGGCAACTGGCCCTCCTCCCTGTCCCCCTCCTTCAAGTCCAAGTCCAT

CCCCTACGGCCGCTTCCAGGTGAAGGCCAACGCCTCCGCCCACCCCAAGGCCAACGGCTC

CGCCGTGAA.CCTGAA.GTCCGGCTCCCTGAACA.CCCA.GGA.GGA.CA.CCTCCTCCTCCCCCCCC

CCCCGCGCCTTCCTGAACCAGCTGCCCGA.CTGGTCCA.TGCTGCTGTCCGCCATCA.CCA.CCG

TGTTCGTGGCCGCCGAGAAGCAGTGGACCATGCTGGACCGCAAGTCCAAGCGCCCCGACA

TGCTGGTGGACTCCGTGGGCCTGAAGAACA.TCGTGCGCGACGGCCTGGTGTCCCGCCA.GT

CCTTCCT GAT CCGCT CCT ACGAGATCGGCGCCGACCGC ACCGCCTCC ATCGAGACCCTGAT

GAACCACCTGCAGGAGACCTCCATCAACCACTGCAAGTCCCTGGGCCTGCTGAACGACGG

CTTCGGCCGCACCCCCGGCATGTGCAAGAACGACCTGATCTGGGTGCTGACCAAGATGCA

GAT CAT GGT GAACCGCT ACCCCGCCTGGGGCGAC ACCGTGG AGATC AAC ACCT GGTTCTC

CCAGTCCGGCAAGATCGGCATGGGCTCCGACTGGCTGATCTCCGACTGCAACACCGGCGA

GATCCTGATCCGCGCCACCTCCGTGTGGGCCATGATGAACCAGAAGACCCGCCGCTTCTCC

CGCCT GCCCT ACGAGGTGCGCC AGGAGCT GACCCCCC ACTTCGT GGACTCCCCCC ACGT G

ATCGAGGACAACGACCGCAAGCTGCACAAGTTCGACGTGAAGACCGGCGACTCCATCCGC

AAGGGCCTGACCCCCCGCTGGAACGACCTGGACGTGAACCAGCACGTGAACAACGTGAA

GTACATCGGCTGGATCCTGGAGTCCACCCCCCCCGAGGTGCTGGAGACCCAGGAGCTGTG

CTCCCTGACCCTGGAGTACCGCCAGGAGTGCGGCCGCGAGTCCGTGCTGGAGTCCCTGAC

CGCCGTGGACCCCTCCGGCAAGGGCTTCGGCTCCCAGTTCCAGCACCTGCTGCGCCTGGA

GGACGGCGGCGAGATCGTGAAGGGCCGCACCGAGTGGCGCCCCAAGACCGCCGGCATCA

ACGGCGCCATCGCCTCCGGCGAGACCTCCCCCGGCGACTTCTGA SEQ ID NO: 100

Cuphea procumbens (Cproc) FATB3 amino acid sequence

MVAAAASSAFFPAPAPGSSPKPGKSGNWPSSLSPSFKSKSIPYGRFQVKANASAHPKANGSAV

NLKSGSLNTQEDTSSSPPPRAFLNQLPDWSMLLSAITTVFVAAEKQWTMLDRKSKRPDMLVD

SVGLKNIVRDGLVSRQSFLIRSYEIGADRTASIETLMNHLQETSINHCKSLGLLNDGFGRTPGM

CKNDLIWVLTKMQIMVNRYPAWGDTVEINTWFSQSGKIGMGSDWLISDCNTGEILIRATSVW

AMMNQKTRRFSRLPYEVRQELTPHFVDSPHVIEDNDRKLHKFDVKTGDSIRKGLTPRWNDLD

VNQHVNNVKYIGWILESTPPEVLETQELCSLTLEYRRECGRESVLESLTAVDPSGEGGYGSQFQ HLLRLEDGGEIVKGRTEWRPKNAGINGVLPTGE* SEQ ID NO: 101

Cuphea procumbens (Cproc) FATB3 coding DNA sequence

ATGGTGGCTGCTGCAGCAAGTTCTGCATTCTTCCCTGCTCCAGCCCCGGGATCCTCACCTA

AACCCGGGAAGTCCGGTAATTGGCCATCGAGCTTGAGCCCTTCCTTCAAGTCCAAGTCAAT

CCCCTATGGCCGATTTCAGGTTAAGGCAAATGCCAGTGCCCATCCTAAGGCTAACGGTTCT

GCAGTAAATCTAAAGTCTGGCAGCCTCAACACTCAGGAGGACACTTCGTCGTCCCCTCCTC

CTCGGGCTTTCCTTAACCAGTTGCCTGATTGGAGTATGCTTCTGTCTGCAATCACGACTGT

ATTCGTGGCGGCAGAGAAGCAGTGGACTATGCTTGATCGGAAATCTAAGAGGCCTGACAT

GCTTGTGGACTCGGTTGGGTTGAAGAATATTGTTCGGGATGGGCTCGTGTCCAGACAGAG TTTTTT(jATTA(jATCTTAT(jAAATAG(jCGCT(jATC(jAACAGCTTCTATA(jA(jACACT(jAT(j

AACCACTTGCAGGAAACATCTATCAATCATTGTAAGAGTTTGGGTCTTCTCAATGACGGCT

TTGGTCGTACTCCTGGGATGTGTAAAAACGACCTCATTTGGGTGCTTACTAAAATGCAGAT

CATGGTGAATCGCTACCCAGCTTGGGGCGATACTGTTGAGATCAATACCTGGTTCTCCCAG

TCGGGGAAAATCGGTATGGGTAGCGATTGGCTAATAAGTGATTGCAACACAGGAGAAATT

CTTATAAGAGCAACGAGCGTGTGGGCCATGATGAATCAAAAAACGAGAAGATTCTCAAG

ACTTCCATACGAGGTTCGCCAGGAGTTAACGCCTCATTTTGTGGACTCTCCTCATGTCATT

GAAGACAATGATCGGAAATTGCATAAGTTCGATGTGAAGACTGGTGATTCTATTCGCAAG

GGTCTAACTCCGAGGTGGAATGACTTGGATGTCAATCAGCACGTCAACAACGTGAAGTAC

ATCGGGTGGATTCTTGAGAGTACTCCACCAGAAGTTCTGGAGACCCAGGAGTTATGTTCCC

TTACCCTGGAATACAGGCGGGAATGTGGAAGGGAGAGCGTGCTGGAGTCCCTCACTGCTG

TGGACCCCTCTGGAGAGGGGGGCTATGGATCCCAGTTTCAGCACCTTCTGCGGCTTGAGG

ATGGAGGTGAGATCGTGAAGGGGAGAACTGAGTGGCGACCCAAGAATGCTGGAATCAAT

GGGGTGTTACCAACCGGGGAGTAG SEQ ID NO: 102

Cuphea procumbens (Cproc) FATB3 coding DNA sequence codon optimized for Prototheca moriformis

ATGGTGGCCGCCGCCGCCTCCTCCGCCTTCTTCCCCGCCCCCGCCCCCGGCTCCTCCCCCA

AGCCCGGCAAGTCCGGCAACTGGCCCTCCTCCCTGTCCCCCTCCTTCAAGTCCAAGTCCAT

CCCCTACGGCCGCTTCCAGGTGAAGGCCAACGCCTCCGCCCACCCCAAGGCCAACGGCTC

CGCCGTGAACCTGAAGTCCGGCTCCCTGAACACCCAGGAGGACACCTCCTCCTCCCCCCCC

CCCCGCGCCTT CCT GAACCAGCT GCCCGACT GGT CC AT GCT GCTGTCCGCCATCACCACCG

TGTTCGTGGCCGCCGAGAAGCAGTGGACCATGCTGGACCGCAAGTCCAAGCGCCCCGACA

TGCT GGT GGACTCCGTGGGCCT GAAGAACAT CGT GCGCGACGGCCT GGTGTCCCGCCAGT

CCTTCCTGATCCGCTCCTACGAGATCGGCGCCGACCGCACCGCCTCCATCGAGACCCTGAT

GAACCACCTGCAGGAGACCTCCATCAACCACTGCAAGTCCCTGGGCCTGCTGAACGACGG

CTTCGGCCGCACCCCCGGCATGTGCAAGAACGACCTGATCTGGGTGCTGACCAAGATGCA

GATCATGGTGAACCGCTACCCCGCCTGGGGCGACACCGTGGAGATCAACACCTGGTTCTC

CCAGTCCGGCAAGATCGGCATGGGCTCCGACTGGCTGATCTCCGACTGCAACACCGGCGA

GATCCTGATCCGCGCCACCTCCGTGTGGGCCATGATGAACCAGAAGACCCGCCGCTTCTCC

CGCCT GCCCT ACGAGGTGCGCC AGGAGCT GACCCCCC ACTT CGT GGACTCCCCCCACGTG

ATCGAGGAC AACGACCGC AAGCT GCACAAGTT CGACGT GAAGACCGGCGACTCCATCCGC

AAGGGCCTGACCCCCCGCTGGAACGACCTGGACGTGAACCAGCACGTGAACAACGTGAA

GTACATCGGCTGGATCCTGGAGTCCACCCCCCCCGAGGTGCTGGAGACCCAGGAGCTGTG

CTCCCTGACCCTGGAGTACCGCCGCGAGTGCGGCCGCGAGTCCGTGCTGGAGTCCCTGAC

CGCCGTGGACCCCTCCGGCGAGGGCGGCTACGGCTCCCAGTTCCAGCACCTGCTGCGCCT

GGAGGACGGCGGCGAGATCGTGAAGGGCCGCACCGAGTGGCGCCCCAAGAACGCCGGCA

TCAACGGCGT GCT GCCCACCGGCGAGT GA SEQ ID NO: 103

Cuphea ignea (Cignea) FATB1 amino acid sequence

PGT SRKTGKF GNWPS SLSPSFKPKSIPNGGF QVKANARAHPKANGS A V SLKS V SLNTQEDTSLS

PPPRAFLNQLPDWRMLRTALTTVFVAAEKQWTMLDRKSKRPDMLVDSFGLESIVQEGLVFRQ

SFSIRSYEIGIDRTASIETLMNHLQETSLNQCKSAGILHDGFGRTLEMCKRDLIWWTKMQIKV

NRYPAWGDTVEISTRFSRLGKIGMGRDWLICDCNTGEILIRATSAYAMMNQKTRRLSKLPNEV

RQEIAPLF VD SDPVIEENDMKLHKFEVKTGDSICKGLTPRW SDLDVNQHV SNVKYIGWILESM

PTEVLETQELCSLALEYRRECGRDSVLESVTSMDPSKVGGWSQYQHLLRLEDGADIVKGRTE

WRPKNAGANGAISTGKT SEQ ID NO: 104

Cuphea ignea (Cignea) FATB1 coding DNA sequence

CCGGGAACCTCACGTAAAACCGGGAAGTTCGGCAATTGGCCATCAAGCTTGAGCCCTTCC

TTCAAGCCCAAGTCAATCCCCAATGGCGGATTTCAGGTTAAGGCTAATGCCAGAGCCCAT

CCTAAGGCTAACGGTTCTGCAGTAAGTCTAAAGTCTGTCAGCCTCAACACTCAGGAGGAC

ACTTCGTTGTCCCCTCCTCCTCGTGCTTTCCTTAACCAGTTGCCTGATTGGAGGATGCTTCG

GACTGCACTCACGACCGTCTTTGTGGCGGCAGAGAAGCAGTGGACTATGCTTGATCGGAA

ATCTAAGAGGCCTGACATGCTCGTGGACTCGTTTGGGTTGGAGAGTATTGTTCAAGAAGG

GCTCGTGTTCAGACAGAGCTTTTCGATTAGGTCTTATGAAATAGGCATTGATCGAACAGCC

TCTATAGAGACGCTGATGAACCACTTGCAGGAAACATCTCTCAATCAATGTAAGAGTGCT

GGTATTCTCCATGACGGCTTCGGTCGTACTCTTGAGATGTGTAAAAGGGACCTCATTTGGG

TTGTTACGAAAATGCAGATCAAGGTGAATCGCTATCCAGCTTGGGGCGATACTGTCGAGA

TCAGTACCCGGTTCTCCCGGTTGGGGAAAATCGGTATGGGTCGCGATTGGCTAATATGTGA

TTGCAACACAGGAGAAATTCTTATAAGAGCAACGAGCGCGTATGCCATGATGAATCAAAA

GACGAGAAGACTCTCAAAACTTCCAAACGAGGTTCGCCAGGAGATAGCGCCTCTTTTTGT

GGACTCTGATCCTGTCATTGAAGAAAATGATATGAAATTGCATAAGTTTGAAGTGAAGAC

TGGTGATTCCATTTGCAAGGGTCTAACTCCGAGGTGGAGTGACTTGGATGTCAATCAGCAC

GTAAGCAACGTGAAGTACATAGGGTGGATTCTCGAGAGTATGCCAACAGAAGTTTTGGAG

ACACAGGAGCTATGCTCTCTCGCCCTTGAATATAGGCGGGAATGCGGAAGGGACAGTGTG

CTGGAGTCTGTGACCTCTATGGATCCCTCAAAAGTTGGAGGCTGGTCTCAGTACCAGCACC

TTCTGCGACTTGAGGATGGGGCGGATATCGTGAAGGGCAGAACTGAGTGGCGGCCGAAG

AAT GCAGGAGCTAACGGGGCGATATC AACAGGAAAG ACTT GA SEQ ID NO: 105

Cuphea ignea (Cignea) FATB1 coding DNA sequence codon optimized for Prototheca moriformis

CCCGGCACCTCCCGCAAGACCGGCAAGTTCGGCAACTGGCCCTCCTCCCTGTCCCCCTCCT

TCAAGCCCAAGTCCATCCCCAACGGCGGCTTCCAGGTGAAGGCCAACGCCCGCGCCCACC

CCAAGGCCAACGGCTCCGCCGTGTCCCTGAAGTCCGTGTCCCTGAACACCCAGGAGGACA

CCTCCCT GTCCCCCCCCCCCCGCGCCTTCCT G AACC AGCT GCCCG ACT GGCGC AT GCT GCG

CACCGCCCTGACCACCGTGTTCGTGGCCGCCGAGAAGCAGTGGACCATGCTGGACCGCAA

GTCCAAGCGCCCCGACATGCTGGTGGACTCCTTCGGCCTGGAGTCCATCGTGCAGGAGGG

CCTGGTGTTCCGCCAGTCCTTCTCCATCCGCTCCTACGAGATCGGCATCGACCGCACCGCC

TCCATCGAGACCCTGATGAACCACCTGCAGGAGACCTCCCTGAACCAGTGCAAGTCCGCC

GGCATCCTGCACGACGGCTTCGGCCGCACCCTGGAGATGTGCAAGCGCGACCTGATCTGG

GTGGTGACCAAGATGCAGATCAAGGTGAACCGCTACCCCGCCTGGGGCGACACCGTGGAG

ATCTCCACCCGCTTCTCCCGCCTGGGCAAGATCGGCATGGGCCGCGACTGGCTGATCTGCG

ACTGCAACACCGGCGAGATCCTGATCCGCGCCACCTCCGCCTACGCCATGATGAACCAGA

AGACCCGCCGCCTGTCCAAGCTGCCCAACGAGGTGCGCCAGGAGATCGCCCCCCTGTTCG

TGGACTCCGACCCCGTGATCGAGGAGAACGACATGAAGCTGCACAAGTTCGAGGTGAAG

ACCGGCGACTCCATCTGCAAGGGCCTGACCCCCCGCTGGTCCGACCTGGACGTGAACCAG

CACGTGTCCAACGTGAAGTACATCGGCTGGATCCTGGAGTCCATGCCCACCGAGGTGCTG

GAGACCCAGGAGCTGTGCTCCCTGGCCCTGGAGTACCGCCGCGAGTGCGGCCGCGACTCC

GTGCTGGAGTCCGTGACCTCCATGGACCCCTCCAAGGTGGGCGGCTGGTCCCAGTACCAG

CACCTGCTGCGCCTGGAGGACGGCGCCGACATCGTGAAGGGCCGCACCGAGTGGCGCCCC

AAGAACGCCGGCGCCAACGGCGCCATCTCCACCGGCAAGACCTGA SEQ ID NO: 106

JcFatBl consensus amino acid sequence

MVAAAASSAFFPVPAPGTSPKPGKSGNWPSSLSPSFKPKSIPNGGFQVKANASAHPKANGSAV

NLKSGSLNTQEDTSSSPPPRAFLNQLPDWSMLLTAITTVFVAAEKQWTMLDRKSKRPDMLVD

SVGLKRIVQDGLVSRQSFSIRSYEIGADRTASIETLMNHLQETSINHCKSLGLLNDGFGRTPGM

CKNDLIWVLTKMQIMVNRYPTWGDTVEINTWFSQSGKIGMGSDWLISDCNTGEILIRATSVW

AMMNQKTRRFSRLPYEVRQELTPHFVDSPHVIEDNDRKLHKFDVKTGDSIRKGLTPRWNDLD

VNQHVSNVKYIGWILESMPIEVLETQELCSLTVEYRRECGMDSVLESVTAMDPSENGGRSQYK HLLRLEDGTDIVKGRTEWRPKNAGTNGAISTGKTSNGNSVS* SEQ ID NO: 107

JcFatBl consensus DNA sequence codon optimized for Prototheca

AT GGT GGCCGCCGCCGCCT CCT CCGCCTT CTTCCCCGTGCCCGCCCCCGGC ACCT CCCCC A

AGCCCGGCAAGTCCGGCAACTGGCCCTCCTCCCTGTCCCCCTCCTTCAAGCCCAAGTCCAT

CCCCAACGGCGGCTTCCAGGTGAAGGCCAACGCCTCCGCCCACCCCAAGGCCAACGGCTC

CGCCGTGAACCTGAAGTCCGGCTCCCTGAACACCCAGGAGGACACCTCCTCCTCCCCCCCC

CCCCGCGCCTTCCTGAACCAGCTGCCCGACTGGTCCATGCTGCTGACCGCCATCACCACCG

TGTTCGTGGCCGCCGAGAAGCAGTGGACCATGCTGGACCGCAAGTCCAAGCGCCCCGACA

TGCTGGTGGACTCCGTGGGCCTGAAGCGCATCGTGCAGGACGGCCTGGTGTCCCGCCAGT

CCTTCTCCATCCGCTCCTACGAGATCGGCGCCGACCGCACCGCCTCCATCGAGACCCTGAT

GAACCACCTGCAGGAGACCTCCATCAACCACTGCAAGTCCCTGGGCCTGCTGAACGACGG

CTTCGGCCGCACCCCCGGCATGTGCAAGAACGACCTGATCTGGGTGCTGACCAAGATGCA

GATCATGGTGAACCGCTACCCCACCTGGGGCGACACCGTGGAGATCAACACCTGGTTCTC

CCAGTCCGGCAAGATCGGCATGGGCTCCGACTGGCTGATCTCCGACTGCAACACCGGCGA

GATCCTGATCCGCGCCACCTCCGTGTGGGCCATGATGAACCAGAAGACCCGCCGCTTCTCC

CGCCTGCCCTACGAGGTGCGCCAGGAGCTGACCCCCCACTTCGTGGACTCCCCCCACGTG

ATCGAGGACAACGACCGCAAGCTGCACAAGTTCGACGTGAAGACCGGCGACTCCATCCGC

AAGGGCCTGACCCCCCGCTGGAACGACCTGGACGTGAACCAGCACGTGTCCAACGTGAAG

TACATCGGCTGGATCCTGGAGTCCATGCCCATCGAGGTGCTGGAGACCCAGGAGCTGTGC

TCCCTGACCGTGGAGTACCGCCGCGAGTGCGGCATGGACTCCGTGCTGGAGTCCGTGACC

GCCATGGACCCCTCCGAGAACGGCGGCCGCTCCCAGTACAAGCACCTGCTGCGCCTGGAG

GACGGCACCGACATCGTGAAGGGCCGCACCGAGTGGCGCCCCAAGAACGCCGGCACCAA

CGGCGCCATCTCCACCGGCAAGACCTCCAACGGCAACTCCGTGTCCTGA SEQ ID NO: 108

JcFatB2 consensus amino acid sequence

MVATAASSAFFPVPSPDTSSRPGKLGNGSSSLSPLKPKSVANGGLQVKANASAPPKINGSSVGL

KSGSLKTQEDTPSAPPPRTFINQLPDWSMLLAAITTVFLAAEKQWMMLDWKPKRPDMLVDPF

GLGRIVQDGLVFRQNFSIRSYEIGADRTASIETVMNHLQETALNHVKSAGLLNDGFGRTPEMY

KRDLIWWAKMQVMVNRYPTWGDTVEVNTWVAKSGKNGMRRDWLISDCNTGEILTRASSV

WVMMNQKTRRLSKIPDEVRHEIEPHFVDSAPVIEDDDRKLPKLDEKTADSIRKGLTPKWNDLD

VNQHVNNVKYIGWILESTPPEVLETQELCSLTLEYRRECGRESVLESLTAVDPSGKGYGSQFQ HLLRLEDGGEIVKGRTE WRPKT AGINGAIASGETSPGDS S * SEQ ID NO: 109

JcFatB2 consensus DNA sequence codon optimized for Prototheca

ATGGTGGCCACCGCCGCCTCCTCCGCCTTCTTCCCCGTGCCCTCCCCCGACACCTCCTCCCG

CCCCGGCAAGCTGGGCAACGGCTCCTCCTCCCTGTCCCCCCTGAAGCCCAAGTCCGTGGCC

AACGGCGGCCTGCAGGTGAAGGCCAACGCCTCCGCCCCCCCCAAGATCAACGGCTCCTCC

GTGGGCCTGAAGTCCGGCTCCCTGAAGACCCAGGAGGACACCCCCTCCGCCCCCCCCCCC

CGCACCTTCATCAACCAGCTGCCCGACTGGTCCATGCTGCTGGCCGCCATCACCACCGTGT

TCCTGGCCGCCGAGAAGCAGTGGATGATGCTGGACTGGAAGCCCAAGCGCCCCGACATGC

TGGTGGACCCCTTCGGCCTGGGCCGCATCGTGCAGGACGGCCTGGTGTTCCGCCAGAACTT

CTCCATCCGCTCCTACGAGATCGGCGCCGACCGCACCGCCTCCATCGAGACCGTGATGAA

CCACCTGCAGGAGACCGCCCTGAACCACGTGAAGTCCGCCGGCCTGCTGAACGACGGCTT

CGGCCGCACCCCCGAGATGTACAAGCGCGACCTGATCTGGGTGGTGGCCAAGATGCAGGT

GATGGTGAACCGCTACCCCACCTGGGGCGACACCGTGGAGGTGAACACCTGGGTGGCCAA

GTCCGGCAAGAACGGCATGCGCCGCGACTGGCTGATCTCCGACTGCAACACCGGCGAGAT

CCTGACCCGCGCCTCCTCCGTGTGGGTGATGATGAACCAGAAGACCCGCCGCCTGTCCAA

GATCCCCGACGAGGTGCGCCACGAGATCGAGCCCCACTTCGTGGACTCCGCCCCCGTGAT

CGAGGACGACGACCGCAAGCTGCCCAAGCTGGACGAGAAGACCGCCGACTCCATCCGCA

AGGGCCTGACCCCCAAGTGGAACGACCTGGACGTGAACCAGCACGTGAACAACGTGAAG

TACATCGGCTGGATCCTGGAGTCCACCCCCCCCGAGGTGCTGGAGACCCAGGAGCTGTGC

TCCCTGACCCTGGAGTACCGCCGCGAGTGCGGCCGCGAGTCCGTGCTGGAGTCCCTGACC

GCCGTGGACCCCTCCGGCAAGGGCTACGGCTCCCAGTTCCAGCACCTGCTGCGCCTGGAG

GACGGCGGCGAGATCGTGAAGGGCCGCACCGAGTGGCGCCCCAAGACCGCCGGCATCAA

CGGCGCC AT CGCCT CCGGCGAGACCT CCCCCGGCGACTCCTCCT GA SEQ ID NO: 110

CuPSR23 FATB3 amino acid sequence

MWAAATSAFFPVPAPGTSPKPGKSGNWPSSLSPTFKPKSIPNAGFQVKANASAHPKA

NGSAVNLKSGSLNTQEDTSSSPPPRAFLNQLPDWSMLLTAITTVFVAAEKQWTMLDRKSKRP

DMLVDSVGLKCIVRDGLVSRQSFLIRSYEIGADRTASIETLMNHLQETSINHCKSLGLLNDGFG

RTPGMCKNDLIWVLTKMQIMVNRYPTWGDTVEINTWFSQSGKIGMASDWLISDCNTGEILIR

ATSVWAMMNQKTRRFSRLPYEVRQELTPHFVDSPHVIEDNDQKLHKFDVKTGDSIRKGLTPR

WNDLDVNQHVSNVKYIGWILESMPIEVLETQELCSLTVEYRRECGMDSVLESVTAVDPSENG

GRSQYKHLLRLEDGTDIVKSRTEWRPKNAGTNGAISTSTAKTSNGNSVS SEQ ID NO: 111

CuPSR23 FATB3b amino acid sequence

MWAAATSAFFPVPAPGTSPKPGKSGNWPSSLSPTFKPKSIPNAGFQVKANASAHPKA

NGSAVNLKSGSLNTQEDTSSSPPPRAFLNQLPDWSMLLTAITTVFVAAEKQWTMLDRKSKRP

DMLVDSVGLKSIVRDGLVSRQSFLIRSYEIGADRTASIETLMNHLQETSINHCKSLGLLNDGFG

RTPGMCKNDLIWVLTKMQIMVNRYPTWGDTVEINTWFSQSGKIGMASDWLISDCNTGEILIR

ATSVWAMMNQKTRRFSRLPYEVRQELTPHFVDSPHVIEDNDQKLHKFDVKTGDSIRKGLTPR

WNDLDVNQHVSNVKYIGWILESMPIEVLETQELCSLTVEYRRECGMDSVLESVTAVDPSENG

GRSQYKHLLRLEDGTDIVKSRTEWRPKNAGTNGAISTSTAKTSNGNSAS SEP ID NO: 112

CwFATB3 amino acid sequence:

MWAAAASSAFFPVPAPRTTPKPGKFGNWPSSLSPPFKPKSNPNGRFQVKANVSPHPK ANGSAVSLKSGSLNTLEDPPSSPPPRTFLNQLPDWSRLRTAITTVFVAAEKQFTRLDRKSKRPD MLVD WF GSETIVQDGLVFRERF SIRS YEIGADRT ASIETLMNHLQDT SLNHCKS V GLLNDGFG RTSEMCTRDLIWVLTKMQIWNRYPTWGDTVEINSWFSQSGKIGMGRDWLISDCNTGEILVR ATSAWAMMNQKTRRFSKLPCEVRQEIAPHFVDAPPVIEDNDRKLHKFDVKTGDSICKGLTPG WNDLDVNQHVSNVKYIGWILESMPTEVLETQELCSLTLEYRRECGRESWESVTSMNPSKVG DRSQYQHLLRLEDGADIMKGRTEWRPKNAGTNRAIST SEP ID NO: 113

CwFATB3a amino acid sequence:

MWAAAASSAFFPVPAPRTTPKPGKFGNWPSSLSPPFKPKSNPNGRFQVKANVSPHPK ANGSAVSLKSGSLNTLEDPPSSPPPRTFLNQLPDWSRLRTAITTVFVAAEKQFTRLDRKSKRPD MLVD WF GSETIVQDGLVFRERF SIRS YEIGADRT ASIETLMNHLQDT SLNHCKS V GLLNDGF G RTSEMCTRDLIWVLTKMQIWNRYPTWGDTVEINSWFSQSGKIGMGRDWLISDCNTGEILVR ATSAWAMMNQKTRRFSKLPCEVRQEIAPHFVDAPPVIEDNDRKLHKFDVKTGDSICKGLTPG WNDLDVNQHVSNVKYIGWILESMPTEVLETQELCSLTLEYRRECGRESWESVTSMNPSKVG DRSQYQHLLRLEDGADIMKGRTEWRPKNAGTNRAIST SEP ID NO: 114 CwFATB3b amino acid sequence

MWAAAASSAFFPVPAPRTTPKPGKFGNWPSSLSPPFKPKSNPNGRFQVKANVSPHPK ANGS A V SLKSGSLNTLEDLPS SPPPRTFLNQLPD W SRLRT AITTVFVAAEKQFTRLDRKSKRPD MLVDWFGSETIVQDGLVFRERFSIRSYEIGADRTASIETLMNHLQDTSLNHCKSVGLLNDGFG RTSEMCTRDLIWVLTKMQIWNRYPTWGDTVEINSWFSQSGKIGMGRDWLISDCNTGEILVR ATSAWAMMNQKTRRFSKLPCEVRQEIAPHFVDAPPVIEDNDRKLHKFDVKTGDSICKGLTPG WNDLDVNQH V SNVKYIGWILEKF WRPRS Y ALSPLNIGGNVEGKVW SEP ID NO: 115

CwFATB3c amino acid sequence

MWAAAASSAFFPVPAPRTTPKPGKFGNWPSSLSPPFKPKSNPNGRFQVKANVSPHPK ANGSAVSLKSGSLNTLEDLPSSPPPRTFLNQLPDWSRLRTAITTVFVATEKQFTRLDRKSKRPD MLVDWFGSETIVQDGLVFRERFSIRSYEIGADRTASIETLMNHLQDTSLNHCKSVGLLNDGFG RTSEMCTRDLIWVLTKMQIWNRYPTWGDTVEINSWFSQSGKIGMGRDWLISDCNTGEILVR ATSAWAMMNQKTRRFSKLPCEVRQEIAPHFVDAPPVIEDNDRKLHKFDVKTGDSICKGLTPG WNDLDVNQH V SNVKYIGWILEKF WRPRS Y ALSPLNIGGNVEGKVW SEP ID NO: 116

CwFATB4a amino acid sequence

MVATAASSAFFPVPSADTSSSRPGKLGSGPSSLSPLKPKSIPNGGLQVKANASAPPKIN

GSSVGLKSGGFKTQEDSPSAPPPRTFINQLPDWSMLLAAITTVFLAAEKQWMMLDWKPKRPD

MLVDPFGLGSIVQDGLVFRQNFSIRSYEIGADRTASIETVMNHLQETALNHVKIAGLSNDGFGR

TPEMYKRDLIWWAKMQVMVNRYPTWGDTVEVNTWVAKSGKNGMRRDWLISDCNTGEILT

RASSVWVMMNQKTRRLSKIPDEVRNEIEPHFVDSAPWEDDDRKLPKLDENTADSIRKGLTPR

WNDLDVNQHVNNVKYIGWILESTPPEVLETQELCSLTLEYRRECGRESVLESLTAVDPSAEGY

ASRFQHLLRLEDGGEIVKARTEWRPKNAGINGWPSEESSPGDFF SEP ID NO: 117

CwFATB4a.l amino acid sequence

MVATAASSAFFPVPSADTSSSRPGKLGSGPSSLSPLKPKSIPNGGLQVKANASAPPKIN

GSSVGLKSGGFKTQEDSPSAPPPRTFINQLPDWSMLLAAITTVFLAAEKQWMMLDWKPKRPD

MLVDPFGLGSIVQDGLVFRQNFSIRSYEIGADRTASIETVMNHLQETALNHVKLAGLSNDGFGR

TPEMYKRDLIWWAKMQVMVNRYPTWGDTVEVNTWVAKSGKNGMRRDWLISDCNTGEILT

RASSVWVMMNQKTRRLSKIPDEVRNEIEPHFVDSAPWEDDDRKLPKLDENTADSIRKGLTPR

WNDLDVNQHVNNVKYIGWILESTPPEVLETQELCSLTLEYRRECGRESVLESLTAVDPSAEGY

ASRFQHLLRLEDGGEIVKARTEWRPKNAGINWWPSEESSPGDFF SEP ID NO: 118

CwFATB4a,2 amino acid sequence:

MVATAASSAFFPVPSADTSSSRPGKLGNGPSSLSPLKPKSIPNGGLQVKANASAPPKIN

GSSVGLKSGSFKTQEDAPSAPPPRTFINQLPDWSMLLAAITTVFLAAEKQWMMLDWKPKRPD

MLVDPFGLGSIVQDGLVFRQNFSIRSYEIGADRTASIETVMNHLQETALNHVKLAGLSNDGFGR

TPEMYKRDLIWWAKMQVMVNRYPTWGDTVEVNTWVAKSGKNGMRRDWLISDCNTGEILT

RASSVWVMMNQKTRRLSKIPDEVRNEIEPHFVDSAPWEDDDRKLPKLDENTADSIRKGLTPR

WNDLDVNQHVNNVKYIGWILESTPPEVLETQELCSLTLEYRRECGRESVLESLTAVDPSAEGY

ASRFQHLLRLEDGGEIVKARTEWRPKNAGINGWPSEESSPGDFF SEP ID NO: 119

CwFATB4a,3 amino acid sequence

MVATAASSAFFPVPSADTSSSRPGKLGSGPSSLSPLKPKSIPNGGLQVKANASAPPKIN

GSSVGLKSGGFKTQEDSPSAPPPRTFINQLPDWSMLLAAITTVFLAAEKQWMMLDWKPKRPD

MLVDPFGLGSIVQDGLVFRQNFSIRSYEIGADRTASIETVMNHLQETALNHVKLAGLSNDGFGR

TPEMYKRDLIWWAKMQVMVNRYPTWGDTVEVNTWVAKSGKNGMRRDWLISDCNTGEILT

RASSVWVMMNQKTRRLSKIPDEVRNEIEPHFVDSAPWEDDDRKLPKLDENTADSIRKGLTPR

WNDLDVNQHVNNVKYIGWILESTPPEVLETQELCSLTLEYRRECGRESVLESLTAVDPSAEGY

VSRFQHLLRLEDGGEIVKARTEWRPKNAGINGWPSEESSPGDFF SEP ID NO: 120

CwFATB4b amino acid sequence

MVATAASSAFFPVPSADTSSSRPGKLGNGPSSLSPLKPKSIPNGGLQVKANASAPPKIN

GSSVGLKSGSFKTQEDAPSAPPPRTFINQLPDWSMLLAAITTVFLAAEKQWMMLDWKPKRPD

MLVDPFGLGSIVQDGLVFRQNFSIRSYEIGADRTASIETVMNHLQETALNHVKIAGLSSDGFGR

TPAMSKRDLIWWAKMQVMVNRYPAWGDTVEVNTWVAKSGKNGMRRDWLISDCNTGEILT

RASSVWVMMNQKTRRLSKIPDEVRNEIEPHFVDSAPWEDDDRKLPKLDENTADSIRKGLTPR

WNDLDVNQHVNNVKYIGWILESTPAEVLETQELCSLTLEYRRECGRESVLESLTAVDPSGEGD

GSKFQHLLRLEDGGEIVKARTEWRPKNAGINGWPSEESSPGGDFF SEP ID NO: 121

CwFATB4b.l amino acid sequence

MVATAASSAFFPVPSADTSSSRPGKLGSGPSSLSPLKPKSIPNGGLQVKANASAPPKIN

GSSVGLKSGSFKTQEDAPSAPPPRTFINQLPDWSMLLAAITTVFLAAEKQWMMLDWKPKRPD

MLVDPFGLGSIVQDGLVFRQNFSIRSYEIGADRTASIETVMNHLQETALNHVKIAGLSSDGFGR

TPAMSKRDLIWWAKMQVMVNRYPAWGDTVEVNTWVAKSGKNGMRRDWLISDCNTGEILT

RASSVWVMMNQKTRRLSKIPDEVRNEIEPHFVDSAPWEDDDRKLPKLDENTADSIRKGLTPR

WNDLDVNQHVNNVKYIGWILESTPAEVLETQELCSLTLEYRRECGRESVLESLTAVDPSGEGD

GSKFQHLLRLEDGGEIVKARTEWRPKNAGINGWPSEESSPGGDFF SEP ID NO: 122

CwFATB5 amino acid sequence

MVAAAASSAFFSVPTPGTPPKPGKFGNWPSSLSVPFKPDNGGFHVKANASAHPKANG SAVNLKSGSLETPPRSFINQLPDLSVLLSKITTVFGAAEKQWKRPGMLVEPFGVDRIFQDGVFF RQSFSIRSYEIGVDRTASIETLMNIFQETSLNHCKSIGLLNDGFGRTPEMCKRDLIWWTKIQVE VNRYPTWGDTIEVNTWVSESGKNGMGRDWLISDCRTGEILIRATSVWAMMNQNTRRLSKFP YEVRQEIAPHFVDSAPVIEDDQKLQKLDVKTGDSIRDGLTPRWNDLDVNQHVNNVKYIGWIL KSVPIEVFETQELCGVTLEYRRECGRDSVLESVTAMDPAKEGDRCVYQHLLRLEDGADITIGR TE WRPKNAGANGAMS SGKT SNGNCLIEGRGW QPFRWRLIF SEP ID NO: 123

CwFATB5a amino acid sequence

MVAAAASSAFFSVPTPGTPPKPGKFGNWPSSLSVPFKPDNGGFHVKANASAHPKANG

SAVNLKSGSLETPPRSFINQLPDLSVLLSKITTVFGAAEKQWKRPGMLVEPFGVDRIFQDGFFFR

QSFSIRSYEIGVDRTASIETLMNIFQETSLNHCKSIGLLNDGFGRTPEMCKRDLIWWTKIQVEV

NRYPTWGDTIEVNTWVSESGKNGMGRDWLISDCRTGEILIRATSVWAMMNQNTRRLSKFPYE

VRQEIAPHFVDSAPVIEDDQKLQKLDVKTGDSIRDGLTPRWNDLDVNQHVNNVKYIGWILKS

VPIEVFETQELCGVTLEYRRECGRDSVLESVTAMDPAKEGDRCVYQHLLRLEDGADITIGRTE

WRPKNAGANGAMSSGKTSNGNCLIEGRGWQPFRWRLIF SEP ID NO: 124

CwFATB5b amino acid sequence

MVAAAASSAFFSVPTPGTPPKPGKFGNWPSSLSVPFKPDNGGFHVKANASAHPKANG SAVNLKSGSLETPPRSFINQLPDLSVLLSKITTVFGAAEKQWKRPGMLVEPFGVDRIFQDGVFF RQSF SIRS YEIGVDRTASIETLMNIF QET SLNHCKSIGLLNDGF GRTPEMCKRDLIWWTKIQVE VNRYPTWGDTIEVNTWVSESGKNGMGRDWLISDCRTGEILIRATSVWAMMNQNTRRLSKFP YEVRQEIAPHFVDSAPVIEDDQKLQKLDVKTGDSIRDGLTPRWNDLDVNQHVNNVKYIGWIL KSVPIEVFETQELCGVTLEYRRECGRDSVLESVTAMDPAKEGDRCVYQHLLWLEDGADITIGR TE WRPKNAGANGAMS SGKT SNGNCLIEGRGW QPFRWRLIF SEP ID NO: 125

CwFATB5c amino acid sequence

MVAAAASSAFFSVPTPGTPPKPGKFGNWPSSLSVPFKPDNGGFHVKANASAHPKANG

SAVNLKSGSLETPPRSFINQLPDLSVLLSKITTVFGAAEKQWKRPGMLVEPFGVDRIFQDGVFF

RQSFSIRSYEIGVDRTASIETLMNIFQETSLNHCKSIGLLNDGFGRTPEMCKRDLIWWTKIQVE

VNRYPIWGDTIEVNTWVSESGKNGMGRDWLISDCRTGEILIRATSVWAMMNQNTRRLSKFPY

EVRQEIAPHFVDSAPVIEDDQKLQKLDVKTGDSIRDGLTPRWNDLDVNQHVNNVKYIGWILK

SVPIEVFETQELCGVTLEYRRECGRDSVLESVTAMDPAKEGDRCVYQHLLRLEDGADITIGRTE

WRPKNAGANGAMSSGKTSNGNCLIEGMGWQPFRWRLIF SEP ID NO: 126

CwFATB5.1 amino acid sequence

MVAAAASSAFFSVPTPGTSPKPGKFRNWPSSLSVPFKPETNHNGGFHIKANASAHPKA

NGSALNLKSGSLETQEDTSLSSPPRTFIKQLPDWSMLLSKITTVFGAAEKQLKRPGMLVEPFGV

DRIFQDGVFFRQSFSIRSYEIGADRTASIETLMNIFQETSLNHCKSIGLLNDGFGRTPEMCKRDLI

WWTKIQVEVNRYPTWGDTIEVNTWVSESGKNGMGRDWLISDCRTGEILIRATSVWAMMNQ

NTRRLSKFPYEVRQEIAPHFVDSAPVIEDDRKLYKLNVKTGDSIRDGLTPRWNDLDVNQHVNN

VKFIGWILKSVPTKVFETQELCGVTLEYRRECGKDSVLESVTAMDPAKEGDRSVYQHLLRLED

GADITIGRTEWRPKNAGANEAISSGKTSNGNSAS SEP ID NO: 127

CwFATB5.1a amino acid sequence

MVAAAASSAFFSVPTPGTSPKPGKFRNWPLSLSVPFKPETNHNGGFHIKANASAHPKA

NGSALNLKSGSLETQEDTSLSSPPRTFIKQLPDWSMLLSKITTVFGAAEKQLKRPGMLVEPFGV

DRIFQDGVFFRQSFSIRSYEIGADRTASIETLMNIFQETSLNHCKSIGLLNDGFGRTPEMCKRDLI

WWTKIQVEVNRYPTWGDTIEVNTWVSESGKNGMGRDWLISDCRTGEILIRATSVWAMMNQ

NTRRLSKFPYEVRQEIAPHFVDSAPVIEDDRKLYKLNVKTGDSIRDGLTPRWNDLDVNQHVNN

VKFIGWILKSVPTKVFETQELCGVTLEYRRECGKDSVLESVTAMDPAKEGDRSVYQHLLRLED

GADITIGRTEWRPKNAGANEAISSGKTSNGNSAS SEP ID NO: 128

CcFATB2b amino acid sequence

MVTTSLASAYFSMKAVMLAPDGRGIKPRSSGLQVRAGNERNSCKVINGTKVKDTEG

LKGCSTLQGQSMLDDHFGLHGLVFRRTFAIRCYEVGPDRSTSIMAVMNHLQEAARNHAESLG

LLGDGFGETLEMSKRDLIWWRRTHVAVERYPAWGDTVEVEAWVGASGNTGMRRDFLVRD

CKTGHILTRCTSVSVMMNMRTRRLSKIPQEVRAEIDPLFIEKVAVKEGEIKKLQKLNDSTADYI

QGGWTPRWNDLDVNQHVNNIIYVGWIFKSVPDSISENHHLSSITLEYRRECIRGNKLQSLTTVC

GGSSEAGIICEHLLQLEDGSEVLRARTEWRPKHTDSFQGISERFPQQEPHK SEP ID NO: 129

CcFATB3 amino acid sequence

MVATAAASAFFPVGAPATSSATSAKASMMPDNLDARGIKPKPASSSGLQVKANAHA

SPKINGSKVSTDTLKGEDTLTSSPAPRTFINQLPDWSMFLAAITTIFLAAEKQWTNLDWKPRRP

DMLADPFGIGRFMQDGLIFRQHFAIRSYEIGADRTASIETLMNHLQETALNHVRSAGLLGDGF

GATPEMSRRDLIWWTRMQVLVDRYPAWGDIVEVETWVGASGKNGMRRDWLVRDSQTGEI

LTRATSVWVMMNKRTRRLSKLPEEVRGEIGPYFIEDVAIIEEDNRKLQKLNENTADNVRRGLT

PRWSDLDVNQHVNNVKYIGWILESAPGSILESHELSCMTLEYRRECGKDSVLQSMTAVSGGG

SAAGGSPESSVECDHLLQLESGPEWRGRTEWRPKSANNSRSILEMPAESL SEP ID NO: 130

CcFATB3b amino acid sequence

MVATAAASAFFPVGAPATSSATSAKASMMPDNLDARGIKPKLASSSGLQVKANAHA

SPKINGSKVSTDTLKGEDTLTSSPAPRTFINQLPDWSMFLAAITTIFLAAEKQWTNLDWKPRRP

DMLADPFGIGRFMQDGLIFRQHFAIRSYEIGADRTASIETLMNHLQETALNHVRSAGLLGDGF

GATPEMSRRDLIWWTRMQVLVDRYPAWGDIVEVETWVGASGKNGMRRDWLVRDSQTGEI

LTRATSVWVMMNKRTRRLSKLPEEVRGEIGPYFIEDVAIIEEDNRKLQKLNENTADNVRRGLT

PRWSDLDVNQHVNNVKYIGWILESAPGSILESHELSCMTLEYRRECGKDSVLQSMTAVSGGG

SAAGGSPESSVECDHLLQLESGPEWRGRTEWRPKSANNSRSILEMPAESL SEP ID NO: 131 CcFATB3c amino acid sequence

MVATAAASAFFPVGAPATSSATSAKASMMPDNLDARGIKPKPASSSGLQVKANAHA SPKINGSKV STDTLKGEDTLTSSPAPRTFINQLPDW SMFLAAITTIFLAAEKQWTNLDWKPRRP DMLADPFGIGRFMQDGLIFRQHFAIRSYEIGADRTASIETLMNHLQETALNHVRSAGLLGDGF GATPEMSRRDLIWWTRMQVLVDRYPAWGDIVEVETWVGASGKNGMRRDWLVRDSQTGEI LTRATSVWVMMNKRTRRLSKLPEEVRGEIGPYFIEDVAIIEEDNRKLQKLNENTADNVRRGLT PRWSDLDVNQHVNNAKYIGWILESAPGSILESHELSCMTLEYRRECGKDSVLQSMTAVSGGG SAAGGSPESSVECDHLLQLESGPEWRGRTEWRPKSANNSRSILEMPAESL SEP ID NO: 132

ChtFATBla amino acid sequence

MVAAAASSAFFSVPTPGTSTKPGNFGNWPSSLSVPFKPESNHNGGFRVKANASAHPK

ANGSAVNLKSGSLETQEDTSSSSPPPRTFIKQLPDWGMLLSKITTVFGAAERQWKRPGMLVEP

FGVDRIFQDGVFFRQSFSIRSYEIGADRTASIETLMNIFQETSLNHCKSIGLLNDGFGRTPEMCK

RDLIWWTKIQVEVNRYPTWGDTIEVNTWVSESGKNGMGRDWLISDCRTGEILIRATSVWAM

MNRKTRRLSKFPYEVRQEIAPHFVDSAPVIEDDKKLHKLDVKTGDSIRKGLTPRWNDLDVNQ

HVNNVKYIGWILKSVPAEVFETQELCGVTLEYRRECGRDSVLESVTAMDTAKEGDRSLYQHL

LRLEDGADITIGRTEWRPKNAGANGAISTGKTSNENSVS SEP ID NO: 133

ChtFATBla.l amino acid sequence

MVAAAASSAFFSVPTPGTSPKPGNFGNWPSSLSVPFKPESNHNGGFRVKANASAHPK

ANGSAVNLKSGSLETQEDTSSSSPPPRTFIKQLPDWGMLLSKITTVFGAAERQWKRPGMLVEP

FGVDRIFQDGVFFRHSFSIRSYEIGADRTASIETLMNIFQETSLNHCKSIGLLNDGFGRTPEMCK

RDLIWWTKIQVEVNRYPTWGDTIEVNTWVSESGKNGMGRDWLIGDCRTGEILIRATSVWAM

MNRKTRRLSKFPYEVRQEIAPHFVDSAPVIEDDKKLHKLDVKTGDSIRKGLTPRWNDLDVNQ

HVNNVKYIGWILKSVPAEVFETQELCGVTLEYRRECGRDSVLESVTAMDTAKEGDRSLYQHL

LRLEDGADITIGRTEWRPKNAGANGALSTGKTSNGNSVS SEP ID NO: 134

ChtFATBla.2 amino acid sequence

MVAAAAS S AFF S VPTPGT SPKPGNFGNWPSNLSVPFKPESNHNGGFRVKANASAHPK ANGSAVNLKSGSLETQEDTSSSSPPPRTFIKQLPDWGMLLSKITTVFGAAERQWKRPGMLVEP FGVDRIFQDGVFFRQSFSIRSYEIGADRTASIETLMNIFQETSLNHCKSIGLLNDGFGRTPEMCK RDLIWWTKIQVEVNRYPTWGDTIEVNTWVSESGKNGMGRDWLISDCRTGEILIRATSVWAM MNRKTRRLSKFPYEVRQEIAPHFVDSAPVIEDDKKLHKLDVKTGDSIRKGLTPRWNDFDVNQ HVNNVKYIGWILKSVPAEVFETQELCGVTLEYRRECGRDSVLESVTAMDTAKEGDRSLYQHL LRLEDGADITIGRTEWRPKNAGANGAISTGKTSNENSVS SEP ID NO: 135

ChtFATBla,3 amino acid sequence

MVAAAASSAFFSVPTPGTSPKPGNFGNWPSSLSVPFKPESNHNGGFRVKANASAHPK ANGSAVNLKSGSLETQEDTSSSSPPPRTFIKQLPDWGMLLSKITTVFGAAERQWKRPGMLVEP F GVDRIF QDGVFFRQSF SIRS YEIGADRTASIETLMNIF QETSLNHCKSIGLLNDGF GRTPEMCK RDLIWWTKIQVEVNRYPTWGDTIEVNTWVSESGKNGMGRDWLISDCRTGEILIRATSVWAM MNRKTRRLSKFPYEVRQEIAPHFVDSAPVIEDDKKLHKLDVKTGDSIRKGLTPRWNDFDVNQ HVNNVKYIGWILKSVPAEVFETQELCGVTLEYRRECGRDSVLESVTAMDTAKEGDRSLYQHL LRLEDGADITIGRTEWRPKNAGVNGAISTGKTSNENSVS SEP ID NO: 136

ChtFATBla.4 amino acid sequence

MVAAAASSAFFSVPTPGTSPKPGNFGNWPSSLSVPFKPESNHNGGFRVKANASAHPK ANGSAVNLKSGSLETQEDTSSSSPPPRTFIKQLPDWSMLLSKITTVFGAAERQWKRPGMLVEPF GVDRIF QDGVFFRQSF SIRS YEIGADRT ASIETLMNIF QET SLNHCKSIGLLNDGF GRTPEMCKR DLIWWTKIQVEVNRYPTWGDTIEVNTWVSESGKNGMGRDWLISDCRTGEILIRATSVWAMM

NRKTRRLSKFPYEVRQEIAPHFVDSAPVIEDDKKLHKLDVKTGDSIRKGLTPRWNDFDVNQHV NNVKYIGWILKSVPAEVFETQELCGVTLEYRRECGRDSVLESVTAMDTAKEGDRSLYQHLLR LEDGADITIGRTE WRPKNAGANGAISTGKT SNEN S V S SEP ID NO: 137

ChtFATBlb amino acid sequence

MVAAAAS S AFF S VPTSGT SPKPGNF GNWP S SLS VPFKPES SHNGGF QVKANAS AHPK ANGSAVNLKSGSLETQEDTSSSSPPPRTFIKQLPDWSMLLSKITTVFWAAERQWKRPGMLVEP F GVDRIF QDGVFFRQSF SIRS YEIGADRTASIETLMNIF QETSLNHCKSIGLLNDGF GRTPEMCK RDLIWWTKIQVEVNRYPTWGDTIEVNTWVSESGKNGMGRDWLISDCRTGEILIRATSVWAM MNRKTRRLSKFPYEVRQEIAPHFVDSAPVIEDDKKLHKLDVKTGDFIRKGLTPRWNDFDVNQ HVNNVKYIGWILKSVPAEVFETQELCGVTLEYRRECGRDSVLESVTAMDTAKEGDRSLYQHL LRLEDGADITIGRTEWRPKNAGANGAISTGKTSNENSVS SEP ID NO: 138

ChtFATB2b amino acid sequence

MWAAAASSAFFPVPASGTSPKPGKFGTWLSSSSPSYKPKSNPSGGFQVKANASAHP KANGSAVSLKSGSLNTQEGTSSSPPPRTFLNQLPDWSRLRTAITTVFVAAEKQLTMLDRKSKK PDMHVDWFGLEIIVQDGLVFRESFSIRSYEIGADRTASIETLMNHLQDTSLNHCKSVGLLNDGF GRTPEMCKRDLIWVLTKMQIMVNRYPT W GDTVEIN SWF SQ SGKIGMGRNWLISDCNTGEILIR ATSIWAMMNQKTRRFSKLPNEVRQEIAPHFVDAPPVIEDNDRKLHKFDVKTGDSICKGLTPEW NDLDVNQHVSNVKYIGWILESMPKEVLDTQELCSLTLEYRRECGRDSVLESVTAMDPSKVGD RSQYQHLLRLEDGTDIMKGRTEWRPKNAGTNGAISTGKTSNGNSVS SEP ID NO: 139

ChtFATB2a amino acid sequence

MWAAAASSAFFPVPAPGTTSKPGKFGNWPSSLSPSFKPKSNPNGGFQVKANASAHP KANGS AV SLKSGSLNTKEDTPSSPPPRTFLNQLPD W SRLRT AITTVF V AAEKQLTMLDRKSKK PDMHVDWFGLEIIVQDWLVFRESFSIRSYEIGADRTASIETLMNHLQDTSLNHCKSVGLLNDGF GRTPEMCKRDLIWVLTKMQIMVNRYPTWGDTVEINSWFSQSGKIGMGRNWLISDCNTGEILIR ATSIWAMMNQKTRRFSKLPNEVRQELAPHFVDAPPLIEDNDRKLHKFDVKTGDSICKGLTPEW NDLDVNQHVSNVKYIGWILESMPKEVLDTQELCSLTLEYRRECGRDSVLESVTAMDPSKVGD RSQYQHLLRLEDGTDIMKGRTEWRPKNAGTNGAISTGKTSNGNSVS SEP ID NO: 140

ChtFATB2c amino acid sequence

MWAAAASSAFFPVPASGTSPKPGKFGTWLSSSSPSYKPKSNPSGGFQVKANASAHP

KANGSAVSLKSGSLNTKEDTPSSPPPRTFLNQLPDWNRLRTAITTVFVAAEKQLTMLDRKSKK

PDMHVDWFGLEIIVQDGLVFRESFSIRSYEIGADRTASIETLMNHLQDTSLNHCKSVGLLNDGF

GRTPEMCKRDLIWVLTKMQIMVNRYPTWGDTVEINSWFSQSGKIGMGRNWLISDCNTGEILIR

ATSIWAMMNQKTRRFSKLPNEVRQEIAPHFVDAPPVIEDNDRKLHKFDVKTGDSICKGLTPEW

NDLDVNQHVSNVKYIGWILESMPKEVLDTQELCSLTLEYRRECGRDSVLESVTAMDPSKVGD

RSQYQHLLRLEDGTDIMKGRTEWRPKNAGTNGAISTGKTSNGNSVS SEP ID NO: 141

ChtFATB2d amino acid sequence

MWAAAASSAFFPVPAPGTTSKPGKFGNWPSSLSPSFKPKSNPNGGFQVKANASAHP

KANGSAVSLKSGSLNTQEDTSSSPPPRTFLNQLPDWSRLLTAISTVFVAAEKQLTMLDRKSKRP

DMLVDLFGLESIVQDGLVFRESYSIRSYEIGADRTASIETLMNHLQDTSLNHCKSVGLLNDGFG

RTPEMCKRDLIWVLTKMQIMVNRYPTWGDTVEINSWFSQSGKIGMGRNWLISDCNTGEILIRA

TSIWAMMNQNTRRFSKLPNEVRQEIAPHFVDAPPVIEDNDRKLHKFDVKTGDSIRKGLTPGWN

DLDVNQHVSNVKYIGWILESMPTEVLETQELCSLTLEYRRECGRESVLESVTAMNPSKVGDRS

QYQHLLRLEDGADIMKGRTEWRPKNAGTNGAISTGKTSNGNSVS SEP ID NO: 142 ChtFATB2e amino acid sequence

MWAAAASSAFFPVPASGTSPKPGKFGTWLSSSSPSYKPKSNPSGGFQVKANASAHP

KANGSAVSLKSGSLNTQEDTSSSPPPQTFLNQLPDWSRLLTAISTVFVAAEKQLTMLDRKSKRP

DMLVDWFGLESIVQDGLVFRESYSIRSYEISADRTASIETVMNLLQETSLNHCKSMGILNDGFG

RTPEMCKRDLIWVLTKMQILVNRYPNWGDTVEINSWFSQSGKIGMGRNWLISDCNTGEILIRA

TSIWAMMNQNTRRFSKLPNEVRQEIAPHFVDAPPVIEDNDRKLHKFDVKTGDSIRKGLTPGWN

DLDVNQHVSNVKYIGWILESMPTEVLETQELCSLTLEYRRECGRDSVLESVTAMNPSKVGDRS

QYQHLLRLEDGADIMKGRTEWRPKNAGTNGAISTGKTSNGNSVS SEP ID NO: 143

ChtFATB2f amino acid sequence

MWAAAASSAFFPVPASGTSPKPGKFGTWLSSSSPSYKPKSNPSGGFQVKANASAHP KANGSAVSLKSGSLNTQEGTSSSPPPRTFLNQLPDWSRLLTAISTVFVAAEKQLTMLDRKSKRP DMLVDWFGLESIVQDGLVFRESYSIRSYEISADRTASIETVMNLLQETSLNHCKSMGILNDGFG RTPEMCKRDLIWVLTKMQILVNRYPNWGDTVEINSWFSQSGKIGMGRNWLISDCNTGEILIRA TSIWAMMNQKTRRFSKLPNEVRQEIAPHFVDAPPVIEDNDRKLHKFDVKTGDSICKGLTPEWN DLDVNQHVSNVKYIGWILESMPKEVLDTQELCSLTLEYRRECGRDSVLESVTAMDPSKVGDR SQY QHLLRLEDGTDIMKGRTE WRPKNAGTNGAIST GKTSNGN S V S SEP ID NO: 144

ChtFATB2g amino acid sequence

MWAATASSAFFPVPVPGTSPKPGKFGTWLSSSSPSYKPKSNPSGGFQVKANASAHPK

ANGSAVSLKSGSLNTQEDTSSSPPPRTFLNQLPDWSRLLTAISTVFVAAEKQLTMLDRKSKRPD

MLVDWFGLESIVQDGLVFREIYSIRSYEISADRTTSIETVMNLLQETSLNHCKSMGILNDGFGRT

PEMCKRDLIWVLTKMQILVNRYPNWGDTVEINSWFSQSGKIGMGRNWLISDCNTGEILIRATSI

WAMMNQKTRRFSKLPNEVRQEIAPHFVDAPPVIEDNDRKLHKFDVKTGDSICKGLTPEWNDL

DVNQHVSNVKYIGWILESMPKEVLDTQELCSLTLEYRRECGRDSVLESVTAMDPSKVGDRSQ

YQHLLRLEDGTDIMKGRTEWRPKNAGTNGAISTGKTSNANSVS SEP ID NO: 145

ChtFATB2h amino acid sequence

MWAAAASSAFFPVPASGTSPKPGKFGTWLSSSSPSYKPKSNPSGGFQVKANASAHP KANGSAV SLKSGSLNTQEGTSSSPPPRTFLNQLPDW SRLLTAISTVFVAAEKQLTMLDRKSKRP DMLVDWFGLESIVQDGLVFRESYSIRSYEISADRTASIETVMNLLQETSLNHCKSMGILNDGFG RTPEMCKRDLIWVLTKMQILVNRYPNWGDTVEINSWFSQSGKIGMGRNWLISDCNTGEILIRA TSIWAMMNQNTRRFSKLPNEVRQEIAPHFVDAPPVIEDNDRKLHKFDVKTGDSIRKGLTPGWN DLDVNQHVSNVKYIGWILESIPTEVLETQELCSLTLEYRRECGRESVLESVTAMNPSKVGDRSQ YQHLLRLEDGADIMKGRTEWRPKNAGTNGAISTGKTSNGNSVS SEP ID NO: 146

ChtFATB3a amino acid sequence

MVATAASSAFFPVPSPDTSSRPGKLGNGSSSLRPLKPKFVANAGLQVKANASAPPKIN GSSV SLKSCSLKTHEDTPSAPPPRTFINQLPDW SMLLAAITTVFLAAEKQWMMLDWKPKRPD MLVDPFGLGRIVQDGLVFRQNFSIRSYEIGADRTASIETVMNHLQETALNHVKSAGLLNEGFG RTPEMYKRDLIWWAKMQVMVNRYPTWGDTVEVNTWVAKSGKNGMRRDWLISDCNTGEIL TRASSVWVMMNQKTRKLSKIPDEVRHEIEPHFVDSAPVIEDDDWKLPKLDEKTADSIRKGLTP KWNDLDVNQHVNNVKYIGWILESTPPEVLETQELCSLTLEYRRECGRESVLESLTAVDPSGKG FGPQFQHLLRLEDGGEIVKGRTEWRPKTAGINGTIASGETSPGNS SEP ID NO: 147

ChtFATB3b amino acid sequence

MVATAASSAFFPVPSPDTSSRPGKLGNGSSSLRPLKPKFVANAGLQVKANASAPPKIN

GSSVSLKSGSLKTQEDTPSAPPPRTFINQLPDWSMLLAAITTVFLAAEKQWMMLDWKPKRPD

MLVDPFGFGRIVQDGLVFRQNFSIRSYEIGADRTASIETVMNHLQETALNHVKSAGLLIEGFGR

TPEMYKRDLIWWAKMQVMVNRYPTWGDTVEVNTWVAKSGKNGMRRDWLISDCNTGEILT

RASSVWVMMNQKTRKLSKIPDEVRHEIEPHFVDSAPVIEDDDWKLPKLDEKTADSIRKGLTPK WNDLDVNQHVNNVKYIGWILESTPPEVLETQELCSLTLEYRRECGRESVLESLTAVDPSGKGF GPQF QHLLRLEDGGEIVKGRTE WRPKT AGINGTIASGET SPGN S SEP ID NO: 148

ChtFATB3c amino acid sequence

MVAT AAS S AFFP VPSPDT S SRPGKLGNGS S SLRPLKPKF VANAGLQVKANAS APPKIN GSSVSLKSCSLKTHEDTPSAPPPRTFINQLPDWSMLLAAITTVFLAAEKQWMMLDWKPKRPD MLVDPF GLGRIVQDGLVFRQNF SIRS YEIGADRT ASIET VMNHLQETALNHVKS AGLLNEGF G RTPEMYKRDLIWWAKMQVMVNRYPTWGDTVEVNTWVAKSGKNGMRRDWLISDCNTGEIL TRASSVWVMMNQKTRKLSKIPDEVRHEIEPHFVDSAPVIEDDDRKLPKLDEKTADSIRKGLTP KWNDLDVNQHVNNVKYIGWILESTPPEVLETQELCSLTLEYRRECGRESVLESLTAVDPSEKG FGPQFQHLLRLEDGGEIVKGRTEWRPKTAGINGAIAFGETSPGDS SEP ID NO: 149

ChtFATB3d amino acid sequence

MVAT AAS S AFFP VPSPDT S SRPGKLGNGS S SLRPLKPKF VANAGLQVKANAS APPKIN GSSVSLKSCSLKTHEDTPSAPPPRTFINQLPDWSMLLAAITTVFLAAEKQWMMLDWKPKRPD MLVDPF GLGRIVQDGLVFRQNF SIRS YEIGADRT ASIKTVMNHLQET ALNHVKSAGLLNEGF G RTPEMYKRDLIWWAKMQVMVNRYPTWGDTVEVNTWVAKSGKNGMRRDWLISDCNTGEIL TRASSVWVMMNQKTRKLSKIPDEVRHEIEPHFVDSAPVIEDDDWKLPKLDEKTADSIRKGLTP KWNDLDVNQHVNNVKYIGWILESTPPEVLETQELCSLTLEYRRECGRESVLESLTAVDPSGKG F GPQF QHLLRLEDGGEIVKGRTE WRPKT AGINGTIASGETSPGNS SEP ID NO: 150

ChtFATB3e amino acid sequence

MVATAASSAFFPVPSPDTSSRPGKLGNGSSSLRPLKPKFVANAGLQVKANAS APPKIN GSSVSLKSGSLKTHEDTPSAPPPRTFINQLPDWSMLLAAITTVFLAAEKQWMMLDWKPKRPD MLVDPFGLGRIVQDGLVFRQNFSIRSYEIGADRTASIETVMNHLQETALNHVKSAGLLNEGFG RTPEMYKRDLIWWAKMQVMVNRYPTWGDTVEVNTWVAKSGKNGMRRDWLISDCNTGEIL TRASSVWVMMNQKTRKLSKIPDEVRHEIEPHFVDSAPVIEDDDWKLPKLDEKTADSIRKGLTP KWNDLDVNQHVNNVKYIGWILESTPPEVLETQELCSLTLEYRRECGRESVLESLTAVDPSGKG FGPQFQHLLRLEDGGEIVKGRTEWRPKTAGINGTLASGETSPGNS SEP ID NO: 151

ChtFATB3f amino acid sequence

MVATAASSAFFPVPSPDTSSRLGKLGNGSSSLRPLKPKFVANAGLQVKANASAPPKIN

GSSVSLKSGSLKTQEDTPSAPPPRTFINQLPDWSMLLAAITTVFLAAEKQWMMLDWKPKRPD

MPVDPFGLGRTVQDGLVFRQNFSIRSYEIGADRTASIETVMNHLQETALNHVKSAGLLNEGFG

RTPEMYKRDLIWWAKMQVMVNRYPTWGDTVEVNTWVAKSGKNGMRRDWLISDCNTGEIL

TRASSVWVMMNQKTRKLSKIPDEVRHEIEPHFVDSAPVIEDDDWKLPKLDEKTADSIRKGLTP

KWNDLDVNQHVNNVKYIGWILESTPPEVLETQELCSLTLEYRRECGRESVLESLTAVDPSEKG

FGPQFQHLLRLEDGGEIVKGRTEWRPKTAGINGTLASGETSPGNS SEP ID NO: 152

ChtFATB3g amino acid sequence

MVATAASSAFFPVPSPDTSSRAGKLGNGSSSLRPLKPKFVANAGLQVKANASAPPKIN

GSSVSLKSGSLKTQEDTPSAPPPRTFINQLPDWSMLLAAITTVFLAAEKQWMMLDWKPKRPD

MLVDPFGLGRTVQDGLVFRQNFSIRSYEIGADRTASIETVMNHLQETALNHVKSAGLLNEGFG

RTPEMYKRDLIWWAKMQVMVNRYPTWGDTVEVNTWVAKSGKNGMRRDWLISDCNTGEIL

TRASSVWVMMNQKTRKLSKIPDEVRHEIEPHFVDSAPVIEDDDWKLPKLDEKTADSIRKGLTP

KWNDLDVNQHVNNVKYIGWILESTPPEVLETQELCSLTLEYRRECGRESVLESLTAVDPSGKG

FGPQFQHLLRLEDGGEIVKGRTEWRPKTAGINGTLASGETSPGNS SEP ID NO: 153 ChsFATBl amino acid sequence

MVATNAAAFSAYTFFLTSPTHGYSSKRLADTQNGYPGTSLKSKSTPPPAAAAARNGA

LPLLASICKCPKKADGSMQLDSSLVFGFQFYIRSYEVGADQTVSIQTVLNYLQEAAINHVQSAG

YFGDSFGATPEMTKRNLIWVITKMQVLVDRYPAWGDWQVDTWTCSSGKNSMQRDWFVRD

LKTGDIITRASSVWVLMNRLTRKLSKIPEAVLEEAKLFVMNTAPTVDDNRKLPKLDGSSADYV

LSGLTPRWSDLDMNQHVNNVKYIAWILESVPQSIPETHKLSAITVEYRRECGKNSVLQSLTNV

SGDGITCGNSIIECHHLLQLETGPEILLARTEWISKEPGFRGAPIQAEKVYNNK SEQ ID NO: 154

ChsFATB2 amino acid sequence

MVATAASSAFFPVPSPDASSRPGKLGNGSSSLSPLKPKLMANGGLQVKANASAPPKIN GSSVGLKSGSLKTQEDTPSAPPPRTFINQLPDWSMLLAAITTVFLAAEKQWMMLDWKPKRPD MLVDPFGLGRIVQDGLVFRQNFSIRSYEIGADRTASIETVMNHLQETALNHVKSAGLLNDGFG RTLEMYKRDLIWWAKMQVMVNRYPTWGDTVEVNTWVAKSGKNGMRRDWLISDCNTGEI LTRASSVWVMMNQKTRRLSKIPDEVRHEIEPHFVDSAPVIEDDDRKLPKLDEKTADSIRKGLTP KWNDLDVNQHVNNVKYIGWILESTPPEVLETQELCSLTLEYRRECGRESVLESLTAVDPSGKG SGSQF QHLLRLEDGGEIVKGRTE WRPKT AGINGPLASGETSPGDS S SEP ID NO: 155

ChsFatB2b amino acid sequence

MVATAASSAFFPVPSPDASSRPGKLGNGSSSLSPLKPKLMANGGLQVKANASAPPKIN GSSVGLKSGSLKTQEDTPSAPPPRTFINQLPDWSMLLAAITTVFLAAEKQWMMLDWKPKRPD MLVDPFGLGRIVQDGLVFRQNFSIRSYEIGADRTASIETVMNHLQETALNHVKSAGLLNDGFG RTLEMYKRDLIWWAKMQVMVNRYPTWGDTVEVNTWVAKSGKNGMRRDWLISDCNTGEI LTRAS SKSQIMLPLHY C S VWVMMNQKTRRLSKIPDEVRHEIEPHFVDSAPVIEDDDRKLPKLD EKTADSIRKGLTPKWNDLDVNQHVNNVKYIGWILESTPPEVLETQELCSLTLEYRRECGRESV LESLTAVDPSGKGSGSQFQHLLRLEDGGEIVKGRTEWRPKTAGINGPLASGETSPGDSS SEP ID NO: 156

ChsFatB2c amino acid sequence

MVATAASSAFFPVPSPDASSRPGKLGNGSSSLSPLKPKLMANGGLQVKANASAPPKIN

GSSVGLKSGSLKTQEDTPSAPPPRTFINQLPDWSMLLAAITTVFLAAEKQWMMLDWKPKRPD

MLVDPFGLGRIVQDGLVFRQNFSIRSYEIGADRTASIETVMNHLQETALNHVKSAGLLNDGFG

RTLEMYKRDLIWWAKMQVMVNRYPTWGDTVEVNTWVAKSGKNGMRRDWLISDCNTGEI

LTRASSVWVMMNQKTRRLSKIPDEVRHEIEPHFVDSAPVIEDDDRKLPKLDEKTADSIRKGLTP

KWNDLDVNQHVNNVKYIGWILESTPPEVLETQELCSLTLEYRRECGRESVLESLTAVDPSGKG

SGSQFQHLMRLEDGGEIVKGRTEWRPKTAGINGPIASGETSPGDSS SEP ID NO: 157

ChsFatB2d amino acid sequence

MVAT AAS S AFFPVPSPDAS SRPGKLGNGS S SLSPLKPKLM ANGGLQVKANAS APPKIN GSSVGLKSGSLKTQEDTPSAPPPRTFINQLPDWSMLLAAITTVFLAAEKQWMMLDWKPKRPD MLVDPFGLGRIVQDGLVFRQNFSIRSYEIGADRTASIETVMNHLQETALNHVKSAGLLNDGFG RTPEMYKRDLIWWAKMQVMVNRYPTWGDTVEVNTWVAKSGKNGMRRDWLISDCNTGEIL TRASSVWVMMNQKTRRLSKIPDEVRHEIEPHFVDSAPVIEDDDRKLPKLDEKTADSIRKGLTP KWNDLDVNQHVNNVKYIGWILESTPPEVLET QELC SLTLE YRRECGRE S VLESLT AVDPSGKG SGSQFQHLLRLEDGGEIVKGRTEWRPKTAGINGPIASGETSPGDSS SEP ID NO: 158

Chs FATB3 amino acid sequence

MVAAEAS S ALF S VRTPGTSPKPGKF GNWPT SLS VPFKSKSNHNGGF QVKANAS ARPK ANGS AV SLKSGSLDTQEDT S S S S SPPRTFINQLPD W SMLLS AITT VF VAAEKQ WTMLDRKSKRP DMLMDPFGVDRWQDGAVFRQSFSIRSYEIGADRTASIETLMNIFQETSLNHCKSIGLLNDGFG RTPEMCKRDLIWWTKMHVEVNRYPTWGDTIEVNTWVSESGKTGMGRDWLISDCHTGEILIR ATSMCAMMNQKTRRFSKFPYEVRQELAPHFVDSAPVIEDYQKLHKLDVKTGDSICNGLTPRW

NDLDVNQHVNNVKYIGWILESVPTEVFETQELCGLTLEYRRECGRDSVLESVTAMDPSKEGD

RSLYQHLLRLEDGADIAKGRTKWRPKNAGTNGAISTGKTSNGNSIS SEP ID NO: 159

ChsFatb3b amino acid sequence

MVAAEASSALFSVRTPGTSPKPGKFGNWPTSLSVPFKSKSNHNGGFQVKANASARPK

ANGSAVSLKSGSLDTQEDTSSSSSPPRTFINQLPDWSMLLSAITTVFVAAEKQWTMLDRKSKRP

DMLMDPFGVDRWQDGAVFRQSFSIRSYEIGADRTASIETLMNIFQETSLNHCKSIGLLNDGFG

RTPEMCKRDLIWWTKMHIEVNRYPTWGDTIEVNTWVSESGKTGMGRDWLISDFHTGDILIR

ATSVCAMMNQKTRRFSKFPYEVRQELAPHFVDSAPVIEDYQKLHKLDVKTGDSICNGLTPRW

NDLDVNQHVNNVKYIGWILESVPTEVFETQELCGLTLEYRRECGRDSVLESVTAMDPSKEGD

RSLYQHLLRLEDGADIAKGRTKWRPKNAGTNGAISTGKTSNGNSIS SEP ID NO: 160

ChsFatB3c amino acid sequence

MVAAEASSALFSVRTPGTSPKPGKFGNWPTSLSVPFKSKSNHNGGFPVKANASARPK

ANGSAVSLKSGSLDTPEDTSSSSSPPRTFINPLPDWSMLLSAITTVFVAAEKPWTMLDRKSKRP

DMLMDPFGVDRWPDGAVFRPSFSIRSYEIGADRTASIETLMNIFPETSLNHCKSIGLLNDGFG

RTPEMCKRDLIWWTKMHVEVNRYPTWGDTIEVNTWVSESGKTGMGRDWLISDCHTGEILIR

ATSMCAMMNPKTRRFSKFPYEVRPELAPHFVDSAPVIEDYPKLHKLDVKTGDSICNGLTPRW

NDLDVNPHVNNVKYIGWILESVPTEVFETPELCGLTLEYRPECGRDSVLESVTAMDPSKEGD

RSLYQHLLRLEDGTDIAKGRTKWRPKNAGKTSNGNSIS SEP ID NO: 161

ChsFATB3d amino acid sequence

MVAAEASSALFSVRTPGTSPKPGKFGNWPSSLSVPFKSKSNHNGGFPVKANASARPK

ANGSAVSLKSGSLDTOEDASSSSSPPRTFINPLPDWSMLLSAITTVFVAAEKPWTMLDRKSKR

SDMLMDPFGVDRWpDGAVFRpSFSIRSYEIGADRTASIETLMNIFpETSLNHCKSIGLLNDGF

GRTPEMCKRDLIWWTKMHVEVNRYPTWGDTIEVNTWVSESGKTGMGRDWLISDCHTGEILI

RATSMCAMMNQKTRRFSKFPYEVRQELAPHFVDSAPVIEDYQKLHKLDVKTGDSICNGLTPR

WNDLDVNQHVNNVKYIGWILESVPTEVFETpELCGLTLEYRRECGRDSVLESVTAMDPSKEG

DRSLYpHLLRLEDGADIAKGRTKWRPKNAGTNGAISTGKTSNGNSIS SEP ID NO: 162

ChsFATB3e amino acid sequence

MVAAE AS S ALF S VRTPGTSPKPGKF GNWPS SLS VPFKSKSNHNGGF QVKANAS ARPK ANGSAVSLKSGSLDTQEDASSSSSPPRTFINpLPDWSMLLSAITTVFVAAEKQWTMLDRKSKR SDMLMDPFGVDRWQDGWFRQSFSIRSYEIGADRTASIETLMNIFQETSLNHCKSIGLLNDGF GRTPEMCKRDLIWWTKMHVE VNRYPT W GDTIE VNT WV SE SGKTGMGRD WLISDCHTGEILI RATSMCAMMNQKTRRFSKFPYEVRQELAPHFVDSAPVIEDYQKLHKLDVKTGDSICNGLTPR WNDLDVNQHVNNVKYIGWILESVPTEVFETOELCGLTLEYRRECGRDSVLESVTAMDPSKEG DRSLYOHLLRLEDGADIAKGRTKWRPKNAGTNGAISTGKTSNGNSIS SEP ID NO: 163

ChsFATB3f amino acid sequence

MVAAE AS S ALF S VRTPGTSPKPGKF GNWPS SLS VPFKSKSNHNGGF QVKANAS ARPK ANGSAVSLKSGSLDTQEDTSSSSSPPRTFINOLPDWSMLLSAITTVFVAAEKQWTMLDRKSKRP DMLMDPFGVDRWQDGAVFRQSFSIRSYEIGADRTASIETLMNIFQETSLNHCKSIGLLNDGFG RTPEMCKRDLIWWTKMHVEVNRYPTWGDTIEVNTWVSESGKTGMGRDWLISDCHTGEILIR ATSMCAMMNQKTRRFSKFPYEVRQELAPHFVDSAPVIEDYQKLHKLDVKTGDSICNGLTPRW NDLDVNOHVNNVKYIGWILESVPTEVFETOELCGLTLEYRRECGRDSVLESVTAMDPSKEGD RSLY PHLLRLEDGADIAKGRTKWRPKNAGTNGAIST GKT SNGN SIS SEP ID NO: 164

ChsFATB3g amino acid sequence

MVAAEAS S ALF S VRTPGTSPKPGKF GNWPT SLS VPFKSKSNHNGGF QVKANAS ARPK

ANGSAVSLKSGSLDTQEDTSSSSSPPRTFINQLPDWSMLLSAITTVFVAAEKQWTMLDRKSKRP

DMLMDPFGVDRWQDGAVFRQSFSIRSYEIGADRTASIETLMNIFQETSLNHCKSIGLLNDGFG

RTPEMCKRDLIWWTKMHIEVNRYPTWGDTIEVNTWVSESGKTGMGRDWLISDFHTGDILIR

ATSVCAMMNQKTRRFSKFPYEVRQELAPHFVDSAPVIEDYQKLHKLDVKTGDSICNGLTPRW

NDLDVNQHVNNVKYIGWILESVPTEVFETQELCGLTLEYRQECGRDSVLESVTAMDPSKEGD

RSLYQHLLRLEDGTDIAKGRTKWRPKNAGKTSNGNSIS SEP ID NO: 165

ChsFATB3h amino acid sequence

MVAAEASSALFSVRTPGTSPKPGKFGNWPSSLSVPFKSKSNHNGGFQVKANASARPK

ANGSAVSLKSGSLDTQEDASSSSSPPRTFINQLPDWSMLLSAITTVFVAAEKQWTMLDRKSKR

SDMLMDPFGVDRWQDGWFRQSFSIRSYEIGADRTASIETLMNIFQETSLNHCKSIGLLNDGF

GRTPEMCKRDLIWWTKMHIEVNRYPTWGDTIEVNTWVSESGKTGMGRDWLISDFHTGDILI

RATSVCAMMNQKTRRFSKFPYEVRQELAPHFVDSAPVIEDYQKLHKLDVKTGDSICNGLTPR

WNDLDVNQHVNNVKYIGWILESVPTEVFETQELCGLTLEYRQECGRDSVLESVTAMDPSKEG

DRSLYQHLLRLEDGTDIAKGRTKWRPKNAGKTSNGNSIS SEP ID NO: 166

ChsFATB3i amino acid sequence

MVAAEASSALFSVRTPGTSPKPGKFGNWPTSLSVPFKSKSNHNGGFPVKANASARPK

ANGSAVSLKSGSLDTPEDTSSSSSPPRTFINPLPDWSMLLSAITTVFVAAEKPWTMLDRKSKRP

DMLMDPFGVDRWPDGAVFRPSFSIRSYEIGADRTASIETLMNIFPETSLNHCKSIGLLNDGFG

RTPEMCKRDLIWWTKMHVEVNRYPTWGDTIEVNTWVSESGKTGMGRDWLISDCHTGEILIR

ATSMCAMMNPKTRRFSKFPYEVRPELAPHFVDSAPVIEDYPKLHKLDVKTGDSICNGLTPRW

NDLDVNOHVNNVKYIGWILESVPTEVFETPELCGLTLEYRRECGGDSVLESVTAMDPSKEGD

RSLYOHLLRLEDGADIAKGRTKWRPKNAGTNGAISTGKTSNGNSIS SEP ID NO: 167

ChsFATB3i amino acid sequence

MVAAEAS S ALF S VRTPGTSPKPGKF GNWPT SLS VPFKSKSNHNGGFQVKANAS ARPK ANGSAVSLKSGSLDTQEDTSSSSSPPRTFINQLPDWSMLLSAITTVFVAAEKQWTMLDRKSKRP DMLMDPFGVDRWQDGAVFRQSFSIRSYEIGADRTASIETLMNIFQETSLNHCKSIGLLNDGFG RTPEMCKRDLIWWTKMHIEVNRYPTWGDTIEVNTWVSESGKTGMGRDWLISDFHTGDILIR ATSVCAMMNQKTRRFSKFPYEVRQELAPHFVDSAPVIEDYQKLHKLDVKTGDSICNGLTPRW NDLDVNQHVNNVKYIGWILESVPTEVFETpELCGLTLEYRQECGRDSVLESVTAMDPSKEGD RSLY QHLLRLEDGTDIAKGRTKWRPKNAGKT SNGN SIS SEP ID NO: 168

CcalcFATBl (Cuphea calcarata FATB1)

MVAAAATS AFFPVPAPGT SPNPRKF GS WPS SLSP SLPKSIPNGGF OVKANAS AHPKANGS AVSL

KSGSLNTQENT S S SPPPRTFLHQLPD W SRLLT AITT VF VKSKRPDMHDRKSKRPDMLVDLF GLE

SSVQDALVFRQSFSIRSYEIGTDRTASIETLMNHLOETSLNHCKSTGILLDGFGRTLEMCKRELI

WWIKMQIQVNRYPAWGDTVEINTRFSRLGKIGMGRDWLISDCNTGEILIRATSEYAMMNQK

TRRLSKLPYEVHQEIAPLFVDSPPVIEDNDLKVHKFEVKTGDSIQKGLSPGWNDLDVNQHVSN

VKYIGWILESMPTEVLETQELCSLALEYRRECGRDSVLESVTAMDPSKVGGRSQYQHLLRLED

GT AIVNGITE WRPKNAGANGAISTGKT SNGN S V S SEP ID NO: 169

ChookFATB4 (Cuphea hookeriana FATB4)

MVAAAATS AFFPVPAPGT SPNPRKF GS WPS SLSP SLPNSIPNGGF OVKANAS AHPKANGS AVSL KSGSLNTQENT S S SPPPRTFLHQLPD W SRLLT AITT VF VKSKRPDMHDRKSKRPDMLVDLF GLE S S V QDALVFRQRF SIRS YEIGTDRTASMETLMNHLQET SLNHCKSTGILLDGF GRTLEMCKREL IWWIKMQIQVNRYPAWGDTVEINTRFSRLGKIGMGRDWLISDCNTGEILIRATSEYAMMNQK TRRLSKLPYEVRQEIAPLFVDSPPVIEDNDLKVHKFEVKTGDSIHKGLTPGWNDLDVNQHVNN

VKYIGWILESTPPEVLETQELCSLTLEYRRECGRESVLESLTAMDPSGGGYGSQFQHLLRLEDG

GEIVKGRTEWRPKNGVINGWPTGESSPGDYS SEP ID NO: 170

CaFATBl (Cuphea avigera var. pulcherrima FATB1)

MVAAAASSAFFSVPVPGTSPKPGKFRIWPSSLSPSFKPKPIPNGGLOVKANSRAHPKANGSAVS

LKSGSLNTQEDTS S SPPPRTFLHQLPD W SRLLT AITTVF VKSKRPDMHDRKSKRPDMLMDSF G

LESIVQEGLEFRQSFSIRSYEIGTDRTASIETLMNYLQETSLNHCKSTGILLDGFGRTPEMCKRDL

IWWTKMKIKVNRYPAWGDTVEINTWFSRLGKIGKGRDWLISDCNTGEILIRATSAYATMNQ

KTRRLSKLPYEVHQEIAPLFVDSPPVIEDNDLKLHKFEVKTGDSIHKGLTPGWNDLDVNQHVS

NVKYIGWILESMPTEVLETQELCSLALEYRRECGRDSVLESVTAMDPTKVGGRSQYQHLLRLE

DGTDIVKCRTEWRPKNPGANGAISTGKTSNGNSVS SEP ID NO: 171

CpauFATBl (Cupheapaucipetala FATB1)

MVAAAASSAFFPVPAPGTSPKPGKSGNWPSSLSPSIKPMSIPNGGFOVKANASAHPKANGSAV

NLKSGSLNTQEDTS SSPPPRAFLNQLPDW SMLLTAITTVFVAAEKQWTMRDRKSKRPDMLVD

SVGLKSWLDGLVSRQIFSIRSYEIGADRTASIETLMNHLQETSINHCKSLGLLNDGFGRTPGMC

KNDLIWVLTKMQIMVNRYPTWGDTVEINTWFSHSGKIGMASDWLITDCNTGEILIRATSVWA

MMNQKTRRFSRLPYEVRQELTPHYVDSPHVIEDNDRKLHKFDVKTGDSIRKGLTPRWNDLDV

NQHVSNVKYIGWILESMPIEVLETQELCSLTVEYRRECGMDSVLESVTAMDPSEDEGRSQYKH

LLRLEDGTDIVKGRTEWRPKNAGTNGAISTAKPSNGNSVS SEP ID NO: 172

CprocFATBl (Cuphea procumbens FATB1)

MVAAAASSAFFPAPAPGSSPKPGKSGNWPSSLSPSFKSKSIPYGRFOVKANASAHPKANGSAV

NLKSGSLNTQEDTSSSPPPRAFLNQLPDWSMLLSAITTVFVAAEKQWTMLDRKSKRPDMLVD

SVGLKNIVRDGLVSRQSFLIRSYEIGADRTASIETLMNHLQETSINHCKSLGLLNDGFGRTPGM

CKNDLIWVLTKMQIMVNRYPAWGDTVEINTWFSQSGKIGMGSDWLISDCNTGEILIRATSVW

AMMNQKTRRFSRLPYEVRQELTPHFVDSPHVIEDNDRKLHKFDVKTGDSIRKGLTPRWNDLD

VNQHVSNVKYIGWILESMPIEVLEAQELCSLTVEYRRECGMDSVLESVTAVDPSEDGGRSQYN

HLLRLEDGTDWKGRTEWRPKNAETNGAISPGNTSNGNSIS SEP ID NO: 173

CprocFATB2 (Cuphea procumbens FATB2)

MVAAAASSAFFPAPAPGSSPKPGKSGNWPSSLSPSFKSKSIPYGRFOVKANASAHPKANGSAV

NLKSGSLNTQEDTSSSPPPRAFLNQLPDWSMLLSAITTVFVAAEKQWTMLDRKSKRPDMLVD

SVGLKNIVRDGLVSRQSFLIRSYEIGADRTASIETLMNHLQETSINHCKSLGLLNDGFGRTPGM

CKNDLIWVLTKMQIMVNRYPAWGDTVEINTWFSQSGKIGMGSDWLISDCNTGEILIRATSVW

AMMNQKTRRFSRLPYEVRQELTPHFVDSPHVIEDNDRKLHKFDVKTGDSIRKGLTPRWNDLD

VNQHVNNVKYIGWILESTPPEVLETQELCSLTLEYRQECGRESVLESLTAVDPSGKGFGSQFQH

LLRLEDGGEIVKGRTEWRPKTAGINGAIASGETSPGDF SEP ID NO: 174

CprocFATB3 (Cuphea procumbens FATB3)

MVAAAASSAFFPAPAPGSSPKPGKSGNWPSSLSPSFKSKSIPYGRFOVKANASAHPKANGSAV

NLKSGSLNTQEDTSSSPPPRAFLNQLPDWSMLLSAITTVFVAAEKQWTMLDRKSKRPDMLVD

SVGLKNIVRDGLVSRQSFLIRSYEIGADRTASIETLMNHLQETSINHCKSLGLLNDGFGRTPGM

CKNDLIWVLTKMQIMVNRYPAWGDTVEINTWFSQSGKIGMGSDWLISDCNTGEILIRATSVW

AMMNQKTRRFSRLPYEVRQELTPHFVDSPHVIEDNDRKLHKFDVKTGDSIRKGLTPRWNDLD

VNQHVNNVKYIGWILESTPPE VLETQELC SLTLEYRRECGRES VLE SLT AVDPSGEGGY GSQFQ

HLLRLEDGGEIVKGRTEWRPKNAGINGVLPTGE SEP ID NO: 175

CigneaFATBl (Cuphea ignea FATB1)

PGT SRKTGKF GNWPS SLSPSFKPKSIPNGGF OVKANARAHPKANGS AY SLKS V SLNTOEDT SLS

PPPRAFLNQLPDWRMLRTALTTVFVAAEKQWTMLDRKSKRPDMLVDSFGLESIVQEGLVFRQ

SFSIRSYEIGIDRTASIETLMNHLQETSLNQCKSAGILHDGFGRTLEMCKRDLIWWTKMQIKV

NRYPAWGDTVEISTRFSRLGKIGMGRDWLICDCNTGEILIRATSAYAMMNQKTRRLSKLPNEV

RQEIAPLF VD SDPVIEENDMKLHKFEVKT GDSICKGLTPRW SDLDVNQHV SNVKYIGWILESM

PTEVLETQELCSLALEYRRECGRDSVLESVTSMDPSKVGGWSQYQHLLRLEDGADIVKGRTE

WRPKNAGANGAISTGKT SEP ID NO: 176

CcalcFATBl (Cuphea calcarata FATB1)

MAT ASTF S AFNARCGDLRRS AGSGPRRPARPLPVRAAI|NASAHPKANGSAV SLKSGSLETQED NS S S SRPPRTFIKQLPD W SMLLSAITTVFVAAEKQWTMFDRKSKRSDMLVDPFWDRIVQDGV LFRQSFSIRSYEIGADRTASIETLMNIFQETSLNHCKSMGLLYEGFGRTPEMCKRDLIWWTKIH IKVNRYPTWGDTIEVTTWVSESGKNGMGRDWLISDCHTGEILIRATSVWAMMNQTTRRLSKF PYELRQEIAPHFVDSDPVIEDNRRLLNFDVKTGDSIRKGLTPRWNDLDVNQHVNNVKYIGWIL ESVPTEVFDTRELCGLTLEYRQECGRGSVLESVTAMDPSKEGDRSLYQHLLRLEDGTDIVKGR TEWRPKNAGTNGPV STRKTTNGSSV S SEP ID NO: 177

ChookFATB4 (Cuphea hookeriana FATB4)

MAT ASTF s afnarcgdlrrsagsgprrparplpvraai|nasahpkangsav SLKSGSLNTQEN

TSSSPPPRTFLHQLPDWSRLLTAITTVFVKSKRPDMHDRKSKRPDMLVDLFGLESSVQDALVFR

QRFSIRSYEIGTDRTASMETLMNHLQETSLNHCKSTGILLDGFGRTLEMCKRELIWWIKMQIQ

VNRYPAWGDTVEINTRFSRLGKIGMGRDWLISDCNTGEILIRATSEYAMMNQKTRRLSKLPYE

VRQEIAPLFVDSPPVIEDNDLKVHKFEVKTGDSIHKGLTPGWNDLDVNQHVNNVKYIGWILES

TPPEVLETQELCSLTLEYRRECGRESVLESLTAMDPSGGGYGSQFQHLLRLEDGGEIVKGRTE

WRPKNGVINGWPTGESSPGDYS SEP ID NO: 178

CaFATBl (Cuphea avigera var. pulcherrima FATB1) matastfsafnarcgdlrrsagsgprrparplpvraaiNsrahpkangsavslksgslntqed

TSSSPPPRTFLHQLPDWSRLLTAITTVFVKSKRPDMHDRKSKRPDMLMDSFGLESIVQEGLEFR

QSFSIRSYEIGTDRTASIETLMNYLQETSLNHCKSTGILLDGFGRTPEMCKRDLIWWTKMKIK

VNRYPAWGDTVEINTWFSRLGKIGKGRDWLISDCNTGEILIRATSAYATMNQKTRRLSKLPYE

VHQEIAPLFVDSPPVIEDNDLKLHKFEVKTGDSIHKGLTPGWNDLDVNQHVSNVKYIGWILES

MPTEVLETQELCSLALEYRRECGRDSVLESVTAMDPTKVGGRSQYQHLLRLEDGTDIVKCRTE

WRPKNPGANGAISTGKTSNGNSVS SEP ID NO: 179

CpauFATBl (Cupheapaucipetala FATB1)

MATASTFSAFNARCGDLRRSAGSGPRRPARPLPVRAAltNASAHPKANGSAVNLKSGSLNTQE

DTSSSPPPRAFLNQLPDWSMLLTAITTVFVAAEKQWTMRDRKSKRPDMLVDSVGLKSWLDG

LVSRQIFSIRSYEIGADRTASIETLMNHLQETSINHCKSLGLLNDGFGRTPGMCKNDLIWVLTK

MQIMVNRYPTWGDTVEINTWFSHSGKIGMASDWLITDCNTGEILIRATSVWAMMNQKTRRFS

RLPYEVRQELTPHYVDSPHVIEDNDRKLHKFDVKTGDSIRKGLTPRWNDLDVNQHVSNVKYI

GWILESMPIEVLETQELCSLTVEYRRECGMDSVLESVTAMDPSEDEGRSQYKHLLRLEDGTDI

VKGRTE WRPKNAGTNGAIST AKPSNGN S V S SEP ID NO: 180

CprocFATBl (Cuphea procumbens FATB1)

MATASTFSAFNARCGDLRRSAGSGPRRPARPLPVRAAltNASAHPKANGSAVNLKSGSLNTQE

DTSSSPPPRAFLNQLPDWSMLLSAITTVFVAAEKQWTMLDRKSKRPDMLVDSVGLKNIVRDG

LVSRQSFLIRSYEIGADRTASIETLMNHLQETSINHCKSLGLLNDGFGRTPGMCKNDLIWVLTK

MQIMVNRYPAWGDTVEINTWFSQSGKIGMGSDWLISDCNTGEILIRATSVWAMMNQKTRRFS

RLPYEVRQELTPHFVDSPHVIEDNDRKLHKFDVKTGDSIRKGLTPRWNDLDVNQHVSNVKYI

GWILESMPIEVLEAQELCSLTVEYRRECGMDSVLESVTAVDPSEDGGRSQYNHLLRLEDGTDV

VKGRTEWRPKNAETNGAISPGNTSNGNSIS SEP ID NO: 181

CprocFATB2 (Cuphea procumbens FATB2)

MATASTFSAFNARCGDLRRSAGSGPRRPARPLPVRAAltNASAHPKANGSAVNLKSGSLNTQE

DTSSSPPPRAFLNQLPDWSMLLSAITTVFVAAEKQWTMLDRKSKRPDMLVDSVGLKNIVRDG

LVSRQSFLIRSYEIGADRTASIETLMNHLQETSINHCKSLGLLNDGFGRTPGMCKNDLIWVLTK

MQIMVNRYPAWGDTVEINTWFSQSGKIGMGSDWLISDCNTGEILIRATSVWAMMNQKTRRFS

RLPYEVRQELTPHFVDSPHVIEDNDRKLHKFDVKTGDSIRKGLTPRWNDLDVNQHVNNVKYI

GWILESTPPEVLETQELCSLTLEYRQECGRESVLESLTAVDPSGKGFGSQFQHLLRLEDGGEIV

KGRTEWRPKTAGINGAIASGETSPGDF SEP ID NO: 182

CprocFATB3 (Cuphea procumbens FATB3)

MATASTFSAFNARCGDLRRSAGSGPRRPARPLPVRAAllNASAHPKANGSAVNLKSGSLNTQE

DTSSSPPPRAFLNQLPDWSMLLSAITTVFVAAEKQWTMLDRKSKRPDMLVDSVGLKNIVRDG

LVSRQSFLIRSYEIGADRTASIETLMNHLQETSINHCKSLGLLNDGFGRTPGMCKNDLIWVLTK

MQIMVNRYPAWGDTVEINTWFSQSGKIGMGSDWLISDCNTGEILIRATSVWAMMNQKTRRFS

RLPYEVRQELTPHFVDSPHVIEDNDRKLHKFDVKTGDSIRKGLTPRWNDLDVNQHVNNVKYI

GWILESTPPEVLETQELCSLTLEYRRECGRESVLESLTAVDPSGEGGYGSQFQHLLRLEDGGEI

VKGRTEWRPKNAGINGVLPTGE SEP ID NO: 183

CigneaFATBl (Cuphea ignea FATB1)_ matastfsafnarcgdlrrsagsgprrparplpvraai|narahpkangsavslksvslntqed

TSLSPPPRAFLNQLPDWRMLRTALTTVFVAAEKQWTMLDRKSKRPDMLVDSFGLESIVQEGL

VFRQSFSIRSYEIGIDRTASIETLMNHLQETSLNQCKSAGILHDGFGRTLEMCKRDLIWWTKM

QIKVNRYPAWGDTVEISTRFSRLGKIGMGRDWLICDCNTGEILIRATSAYAMMNQKTRRLSKL

PNEVRQELAPLFVDSDPVIEENDMKLHKFEVKTGDSICKGLTPRW SDLDVNQHV SNVKYIGWI

LESMPTEVLETQELCSLALEYRRECGRDSVLESVTSMDPSKVGGWSQYQHLLRLEDGADIVK

GRTEWRPKNAGANGAISTGKT SEP ID NO: 184

CgFATBl (Cuphea glossostoma FATB1)_ MVAAAASSAFFPSPAPGSSPKPGNRPSSLSPSFKPKSIPNGAFQVKANASAHPKANGSAVNLKSl

GSLNTQEDSSSSPSPRAFLNQLPDWSVLLTAITTVFVAAEKQWTMLDRKSKRPDVLVDSVGLK SIVQDGLVSRQSFSIRSYEIGADRTASIETLMNHLQETSINHCKSLGLLNDGFGRTPGMCKNDLl|

wvltkmqimvnrypawgdtveintwfsqsgkigmgsdwlisdcntgeiliratsvwammnqI

KTRRFSRLPYEVRQELTPHFVDSPHVIEDNDRKLHKFDVKTGDSIRKGLTPRWNDLDVNQHVS

NVKYIGWILESMPIEVLETQELCSLTVEYRRECGMDSVLESVTAMDPSEDGGRSQYNHLLRLEI DGTDWKGRTEWRPKNAGTNGAISTTKTSNGNSVSl SEP ID NO: 185

CgFATBlb (Cuphea glossostoma FATB1 C170F,M198T,T374S variant)

MVAAAASSAFFPSPAPGSSPKPGNRPSSLSPSFKPKSIPNGAFQVKANASAHPKANGSAVNLKS

GSLNTQEDSSSSPSPRAFLNQLPDWSVLLTAITTVFVAAEKQWTMLDRKSKRPDVLVDSVGLK

SIVQDGLVSRQSFSIRSYEIGADRTASIETLMNHLQETSINHFKSLGLLNDGFGRTPGMCKNDLI

WVLTKTQIMVNRYPAWGDTVEINTWFSQSGKIGMGSDWLISDCNTGEILIRATSVWAMMNQ

KTRRFSRLPYEVRQELTPHFVDSPHVIEDNDRKLHKFDVKTGDSIRKGLTPRWNDLDVNQHVS

NVKYIGWILESMPIEVLETQELCSLTVEYRRECGMDSVLESVSAMDPSEDGGRSQYNHLLRLE

DGTDWKGRTEWRPKNAGTNGAISTTKTSNGNSVS SEP ID NO: 186

Umbellularia califomica UcFATB3 amino acid sequence

MVATAAASAFFPVGSPATSSATSAKASMMPDNLDARGIKPKPASSSGLQVKANAHASPKINGS

KV STDTLKGEDTLTSSPAPRTFINQLPDW SMFLAAITTIFLAAEKQWTNLDWKPRRPDMLADP

FGIGRFMQDGLIFRQHFAIRSYEIGADRTASIETLMNHLQETALNHVRSAGLLGDGFGATPEMS

RRDLIWWTRMQVLVDRYPAWGDIVEVETWVGASGKNGMRRDWLVRDSQTGEILTRATSV

WVMMNKRTRRLSKIPEEVRGEIGPYFMENVAIIEEDSRKLQKLNENIIEEDSRKLQKLNENTAD

NVRRGLTPRWSDLDVNQHVNNVKYIGWILESAPGSILESHELSCMTLEYRRECGKDSVLQSM TVVSGGGSAAGGSPESSVECDHLLQLESGPEWKARTEWRPKSANNPRSILEMPAESS* SEP ID NO: 187

Cuphea carthagenensis CCrFATB2c (V138L variant of FATB2)

MVAAAASSAFFPVTTPGTSRKPGKFGNWLSSLSPPFRPKSIPSGGFQVKANASAHPKANGSAV

SLKSGSLNTQEDTSSSPPPRAFINQLPDWSMLLTAITTVFVAAEKQWTMLDRKSKRSDMLVDS

FGMERIVQDGLLFRQSFSIRSYEIGADRRASIETLMNHLQETSLNHCKSIRLLNEGFGRTPEMCK

RDLIWWTRMHIMVNRYPTWGDTVEINTWVSQSGKNGMGRDWLISDCNTGEILIRATSAWA

MMNQKTRRLSKLPYEVSQEIAPHFVDSPPVIEDGDRKLHKFDVKTGDSIRKGLTPRWNDLDV

NQHVNNVKYIGWILESMPTEVLETHELCFLTLEYRRECGRDSVLESVTAMDPSNEGGRSHYQ HLLRLEDGTDIVKGRTEWRPKNARNIGAISTGKTSNGNPAS* SEP ID NO: 188

Cuphea carthagenensis CCrFATB2

MVAAAASSAFFPVTTPGTSRKPGKFGNWLSSLSPPFRPKSIPSGGFQVKANASAHPKANGSAV

SLKSGSLNTQEDTSSSPPPRAFINQLPDWSMLLTAITTVFVAAEKQWTMLDRKSKRSDMLVDS

FGMERIVQDGLVFRQSFSIRSYEIGADRRASIETLMNHLQETSLNHCKSIRLLNEGFGRTPEMCK

RDLIWWTRMHIMVNRYPTWGDTVEINTWVSQSGKNGMGRDWLISDCNTGEILIRATSAWA

MMNQKTRRLSKLPYEVSQEIAPHFVDSPPVIEDGDRKLHKFDVKTGDSIRKGLTPRWNDLDV

NQHVNNVKYIGWILESMPTEVLETHELCFLTLEYRRECGRDSVLESVTAMDPSNEGGRSHYQ HLLRLEDGTDIVKGRTEWRPKNARNIGAISTGKTSNGNPAS* SEP ID NO: 189

CcrFATB2b

MVAAAASSAFFPVTTPGTSRKPGKFGNWLSSLSPPFRPKSIPSGGFQVKANASAHPKANGSAV

SLKSGSLNTQEDTSSSPPPRAFINQLPDWSMLLTAITTVFVAAEKQWTMLDRKSKRSDMLVDS

FGMERIVQDGLVFRQSFSIRSYEIGADRRASIETLMNHLQETSLNHCKSIRLLNEGFGRTPEMCK

RDLIWVFTRMHIMVNRYPTWGDTVEINTWVSQSGKNGMGRDWLISDCNTGEILIRATSAWA

MMNQKTRRLSKLPYEVSQEIAPHFVDSPPVIEDGDRKLHKFDVKTGDSIRKGLTPRWNDLDV

NQHVNNVKYIGWILESMPTEVLETHELCFLTLEYRRECGRDSVLESVTAMDPSNEGGRSHYQ HLLRLEDGTDIVKGRTEWRPKNARNIGAIPTGKTSNGNPAS* SEP ID NO: 190 CcrFATBl

MVATAASSAFFPVPSPDSSSRPGKLGNGPSSLSPLKPKSTPNGGLQVKANASAPPKINGSSVGL

KSSSLKTQDDTPSAPPPRTFINQLPDWSMLLAAITTVFLAAEKQWMMLDWKPKRPDMLTDPF

GLGRIVQDGLVFRQNFSIRSYEIGADRTASIETVMNHLQETALNHVKSAGLLNDGFGRTPEMY

KRDLIWWAKMQVMVNRYPTWGDTVEVNTWVAKSGKNGMRRDWLISDCNTGEILTRASSV

WVMMNQKTRRLSKIPDEVRHEIEPHFVDSAPVIEDDDRKLPKLDEKTADSIRKGLTPKWNDLD

VNQHVNNVKYIGWILESTPPEVLETQELCSLTLEYRRECGKESVLESLTAVDPSGKGWGSHFQ HLLRLEDGGEIVKGRTEWRPKNAGINGAVAFEETSPGDS* SEP ID NO: 191

CcrFATBlb

MVATAASSAFFPVPSPDSSSRPGKLGNGPSSLSPLKPKSTPNGGLQVKANASAPPKINGSSVGL

KSSSLKTQDDTPSAPPPRTFINQLPDWSMLLAAITTVFLAAEKQWMMLDWKPKRPDMLTDPF

GLGRIAQDGLVFRQNFSIRSYEIGADRTASIETVMNHLQETALNHVKSAGLLNDGFGRTPEMY

KRDLIWWAKMQVMVNRYPTWGDTVEVNTWVAKSGKNGMRRDWLISDCNTGEILTRASSV

WVMMNQKTRRLSKIPDEVRHEIEPHFVDSAPVIEDDDRKLPKLDEKTADSIRKGLTPKWNDLD

VNQHVNNVKYIGWILESTPPEVLETQELCSLTLEYRRECGKESVLESLTAVDPSGKGWGSHFQ HLLRLEDGGErVKGRTEWRPKNAGINGAVAFEETSPGDS* SEP ID NO: 192 CCrFATBlc

MVATAASSAFFPVPSPDSSSRPGKLGNGPSSLSPLKPKSTPNGGLQVKANASAPPKINGSSVGL

KSSSLKTQDDTPSAPPPRTFINQLPDWSMLLAAITTVFLAAEKQWMMLDWKPKRPDMLTDPF

GLGRIVQDGLVFRQNFSIRSYEIGADRTASIETVMNHLQETALNHVKSAGLLNDGFGRTPEMY

KRDLIWWAKMQVMVNRYPTWGDTVEVNTWVAKSGKNGMRRDWLISDCNTGEILTRASSV

WVMMNQKTRRLSKIPDEVRHEIEPHFVDSAPVIEDDDRKLPKLDEKTADSIRKGLTPKWNDLD

VNQHVNNVKYIGWILESTPPEVLETQELCSLTLEYRRECGKESVLESLTAVDPSGKGWGSHFQ HLLRLEDGGEIVKGRTEWRPKNA*

Claims (16)

1. THE CLAIMS DEFINING THE INVENTION ARE AS FOLLOWS:

   1. A recombinant nucleic acid encoding an acyl-ACP thioesterase, wherein the nucleic acid has at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, or 99% sequence identity to SEQ ID NO: 93, and wherein the nucleic acid encodes an acyl-ACP thioesterase having at least 96% sequence identity to SEQ ID NOs: 91, 171 or 179, or a C-terminal and/or N-terminal truncated fragment comprising at least 90% of the full-length sequences thereof.
2. 2. A vector comprising the recombinant nucleic acid of claim 1.
3. 3. A method of producing a recombinant acyl-ACP thioesterase, the method comprising transforming a Prototheca microalgal cell with a nucleic acid according to claim 1.
4. 4. A method of producing a recombinant acyl-ACP thioesterase, the method comprising transforming a Prototheca microalgal cell with a nucleic acid encoding an acyl-ACP thioesterase having at least 96% sequence identity to SEQ ID NOs: 91, 171 or 179 or a C-terminal and/or N-terminal truncated fragment comprising at least 90% of the full-length sequences thereof.
5. 5. A Prototheca microalgal host cell comprising a recombinant nucleic acid of claim 1, wherein the recombinant nucleic acid comprises an exogenous nucleic acid, and wherein the host cell has a fatty acid profile having increased C8 and/or CIO fatty acids.
6. 6. A Prototheca microalgal host cell comprising a recombinant nucleic acid encoding an acyl-ACP thioesterase having at least 96% sequence identity to SEQ ID NOs: 91, 171 or 179 or a C-terminal and/or N-terminal truncated fragment comprising at least 90% of the full-length sequences thereof, and wherein the host cell has a fatty acid profile having increased C8 and/or CIO fatty acids.
7. 7. A method for producing an oil or oil-derived product, the method comprising cultivating a host cell of claim 5 or claim 6, and extracting oil produced thereby.
8. 8. The method of claim 7, wherein the cultivation is heterotrophic growth on sugar.
9. 9. The method of claim 7 or claim 8, further comprising producing a fatty acid, fuel, chemical, or other oil-derived product from the oil.
10. 10. An oil produced by the method of claim 7 or claim 8.
11. 11. The oil of claim 10 having a fatty acid profile comprising at least about 8% C8 and/or at least about 9% CIO fatty acids.
12. 12. An oil-derived product produced by the method of claim 9.
13. 13. The oil-derived product of claim 12, wherein the oil is produced by a microalga and lacks C24-alpha sterols.
14. 14. The oil-derived product of claim 13, wherein the oil comprises sterols with C24P stereochemistry.
15. 15. The oil-derived product of claim 13 or 14, wherein the oil comprises one or more of ergosterol, brassicasterol, 22, 23-dihydrobrassicasterol, clionasterol and poriferasterol.
16. 16. The oil-derived product of any one of claims 14 to 15, wherein the oil is free from one or more of β-sitosterol, campesterol, or stigmasterol.