AU2013203523B2 - Fluorination of aromatic ring systems - Google Patents

Abstract

This disclosure relates to reagents and methods useful in the synthesis of aryl fluorides, for example, in the preparation of 18F labeled radiotracers. The reagents and methods provided herein may be used to access a broad range of compounds, including aromatic compounds, heteroaromatic compounds, amino acids, nucleotides, and synthetic compounds.

Description

Fluorination of Aromatic Ring Systems FEDERALLY SPONSORED RESEARCH OR DEVELOPMENT The U S. Government has certain rights in this invention pursuant to Grant No. CH E-0717562 awarded by the National Science Foundation CROSS-REFERENCE TO RELATED APPLICATIONS 5 This application claims priority to U.S. Provisional Applications Serial Nos. 61/107,156, filed on October 21, 2008, and 61/236,037, filed on August 21, 2009, both of which are incorporated by reference in their entirety herein. TECHNICAL FIELD This disclosure relates to reagents and methods useful in the synthesis of aryl 10 fluorides, for example, in the preparation of 1 labeld radiotracers. The reagents and methods provided herein may be used to access a broad range of compounds, including aromatic compounds, heteroaromatic compounds, amino acids, nucleotides, and synthetic compounds. BACKGROUND 15 Aryl fluorides are structural moieties in natural products as well as a number of therapeutically important compounds, including positron emission tomography (PET) tracers and pharmaceuticals. Therefore methods and reagents for producing such aryl fluorides, for example efficient methods for producing aryl fluorides, are desirable. 20 Any discussion of the prior art throughout the specification should in no way be considered as an admission that such prior art is widely known or forms part of common general knowledge in the field. It is an object of the present invention to overcome or ameliorate at least one of the disadvantages of the prior art, or to provide a useful alternative. 25 Unless the context clearly requires otherwise, throughout the description and the claims, the words "comprise", "comprising", and the like are to be construed in an inclusive sense as opposed to an exclusive or exhaustive sense; that is to say, in the sense of "including, but not limited to".

SUMMARY According to a first aspect of the invention there is provided a method for making a compound of Formula (3): Ar 2 -F 3 wherein: (A) Ar is an aryl or heteroaryl ring system; the method comprising reacting in a polar solvent a compound MF, wherein 1I M is a counter ion, and a compound of Formula (2): Y ArI- Ar 2 2 wherein: Arl is an electron rich aryl or heteroaryl ring system; 15 Y is a leaving group; and

A

2 is as defined above; removing the polar solvent from the reaction mixture; combining the remaining mixture wih a nonpolar solvent; filtering the resulting mixture to remove insoluble material; and 20 heating the filtrate; (B) Ar is an aryl or heteroaryl ring system; the method comprising reacting in a nonpolar solvent a compound ME wherein M is a counter ion, and a compound of Formula (2): y Ar- I Ar2 25 2 wherein: Ar' is an electron rich aryl or heteroaryl ring system; y is a leaving group; and ArI is as defined above; 30 filtering the reaction mixture to remove insoluble material and Ia heating the filtrate; (C) Ar 2 is an aryl or heteroaryl ring system; the method comprising reacting in a polar solvent a compound ME, 5 wherein M is a counter ion, and a compound of Formula (2): Y Ar 1 Ar 2 wherein: Ar' isan electron rich aryl or heteroaryl ring system; 10 Y is a leaving group; and Ar 2 is as defined above: removing the polar solvent from the reaction mixture; and heating a solution comprising the remaining mixture and a nonpolar solvent. According to a second aspect of the invention there is provided a product obtained by the method of the invention, Provided herein are methods of preparing substituted aryl and heteroaryl ring 20 systems using diaryliodonium compounds and intermediates For example, diaryliodonium salts and diaryliodonium fluorides, as provided herein, can undergo decomposition to prepare an aryl fluoride. lb For example, provided herein is a method for making a compound of Formula (1): Ar 2 -X 1 wherein Ax is an aryl or heteroaryl ring system; and X is a moiety wherein the pKa of 5 the acid H-X is less than 12. In one embodiment, the method includes reacting in a polar solvent a compound MX, wherein M is a counter ion and X is as defined in Formula (1), and a compound of Formula (2): Arl- AAr2 2 10 wherein Arl is an electron rich aryl or heteroaryl ring system; Y is a leaving group; and Ar 2 and X are as defined above. Following reaction, the polar solvent can be removed from the reaction mixture and the remaining mixture can be combined with a nonpolar solvent and heated. In another embodiment, a solution comprising a 15 nonpolar solvent, a compound MX, and a compound of Formula (2) can be heated to provide a compound of Formula (1). In some embodiments, the nonpolar solution of the reaction mixture of MX and a compound of Formula (2) can be filtered prior to heating. The filtration step can remove any insoluble material (e.g., insoluble salts) that remain in the reaction 20 mixture. In some embodiments, the solvent can be removed from the filtrate prior to heating (i.e., the residue can be heated neat). In further embodiments, the nonpolar solution of the reaction mixture of MX and a compound of Formula (2) can be filtered prior to heating, the nonpolar solvent can be removed (e.g., by evaporation), and the heating of the sample can be 25 performed in a different solvent. In some embodiments, X can be chosen from halide, aryl carboxylate, aLkyl carboxylate, phosphate, phosphonate, phosphonite, azide, thiocyanate, cyanate, phenoxide, triflate, trifluoroethoxide, thiolates, and stabilized enolates. For example, X can be chosen from fluoride, chloride, bromide, iodide, triflate, trifluoroacetate, 30 benzoate, acetate, phenoxide, trifluoroethoxide, cyanate, azide, thiocyanate, thiolates, phosphates, and stabilized enolates. In some embodiments, X is fluoride. In some embodiments, X is a radioactive isotope, for example, X can be a radioactive isotope of fluoride (e.g., 1 8 F). 2 The methods described herein can be used to prepare fluorinated aryl or heteroaryl ring systems (e.g., a radiolabeled fluorinated aryl or heteroaryl ring system). For example, provided herein is a method of preparing a compound of Formula (3): Ar 'F 5 3 wherein Ar2 is an aryl or heteroaryl ring system. In one embodiment, the method includes reacting in a polar solvent a compound MF, wherein M is a counter ion, and a compound of Formula (2), as described above. Following reaction, the polar solvent 10 can be removed from the reaction mixture and the remaining mixture can be combined with a nonpolar solvent and heated. In another embodiment, a solution comprising a nonpolar solvent, a compound MF, and a compound of Formula (2) can be heated to provide a compound of Formula (3). In some embodiments, the nonpolar solution of the reaction mixture of MF 15 and a compound of Formula (2) can be filtered prior to heating. The filtration step can remove any insoluble material (e.g., insoluble salts) that remain in the reaction mixture. In some embodiments, the solvent can be removed from the filtrate prior to heating (i.e., the residue can be heated neat). In further embodiments, the nonpolar solution of the reaction mixture of MF 20 and a compound of Formula (2) can be filtered prior to heating, the nonpolar solvent can be removed (e.g., by evaporation), and the heating of the sample can be performed in a different solvent. Ar' is an electron rich aryl or heteroaryl ring system. For example, Ar'-H can be more easily oxidized than benzene. In some embodiments, the moiety Ar' can be 25 substituted with at least one substituent having a Hammett op value of less than zero. For example, the substituent can be chosen from: -(C-C 1 o)alkyl, -(C-Cio)haloalkyl,

(C

2 -Cio)atkenyl, (C 2 -Cio)alkynyl, -O-(Ci-Cio)alkyl, -C(O)-O-(C,-Cio)alkyl, aryl, and heteroaryl. In some embodiments, Ar' can be:

R

2

R

1 R3 q

R

4

R

5 30 wherein R, R 2 , R 3 , R4, and R 5 are independently chosen from: H, -(Ci-Cio)alkyl,

-(C

Cio)haloalkyl, (C 2 -Cio)alkenyl, (C 2 -C1O)alkynyl, -O-(C-Cio)alkyl, -C(O)-O-(C 3 Cio)alkyl, aryl, and heteroaryl, or two or more of R', R2, R 3 , R 4 , and R come together to form a fused aryl or hoteroaryl ring system. Ar2 is an aryl or heteroaryl ring system. In some embodiments, Ar2 is chosen from a phenylalanine derivative, tyrosine derivative, typtophan derivative, histidine 5 derivative, and estradiol derivative. In some embodiments, Ar2 is chosen from: OMe CN MeO OMe Me OMe OMe NCF 3 4 NI D2 p 2 P1. 2 N"p N IIN

OP

4 OP pN p 2 PvN' 2 lN'p O, p5 O, p5 Olp B IOP 5,0 3 - Op 3 o P1 '2Pt 1-NIp 2 PIN P 2 O-p5 cxP 6 0 'p5 0 ~ 0 pN 1 0p 2 pt y2 NJ N p 6

P

6 5 lt p 2 N'

P

2 P1'N N'P 2

OP

3 N O N NN P PB

P

6 S Pt, N 2 Pt N 22 PIt p 2 N N N o 0 0 N N 'P6 N>PS '6 Nl N iIN'p 1I pp 2 p\3' 0N N plt N22 pt, N'2 Pt, 2 6 N N P p 6 p6 6 Ft, N'p 2 Pt, Np 2 1,N p N N N p6 p6 'p6 P N P N N p2 1,N 2P1' N' 7 7-O p7- P -O OP3 P3 Op 3 P3 P -0 p7-0 7 OP3 OP3 OP4 CN N p N N CN CN CN

P

7 -0p 7 -0p 7 -0p 7 -0( SN /)N OP CN Op3 CN p4-0 wherein each of P1, p2 and p6 are independently a nitrogen protecting group, or P1 and p2 come together to form a single nitrogen protecting group; each of p3, p4 , and p7 are 7 independently an alcohol protecting group, or P 3 and P 4 come together to form a single oxygen protecting group; and P 5 is a carboxylic acid protecting group. Also provided herein is a method of making a compound of Formula (6): PN OIP F N 0 Op 3 Op 4 5 6 wherein each of Pland P 2 are independently a nitrogen protecting group, or P1 and P2 come together to form a single nitrogen protecting group; each of p3, and P 4 are independently an alcohol protecting group, or P 3 and P 4 come together to form a single oxygen protecting group; and P 5 is a carboxylic acid protecting group. In one 10 embodiment, the method includes reacting in a polar solvent a compound MF, wherein M is a counter ion, and a compound of Formula (7): P N Y ONpS Ar 0 Op 3

OP

4 7 wherein Ar' is an electron rich aryl or heteroaryl ring system; Y is a leaving group; 15 and

P',P

2 3, P4, P and P 5 are as defined above. Following reaction, the polar solvent can be removed from the reaction mixture and the remaining mixture can be combined with a nonpolar solvent and heated. In another embodiment, a solution comprising a nonpolar solvent, a compound MF, and a compound of Formula (7) can be heated to 20 provide a compound of Formula (6). In some embodiments, the nonpolar solution of the reaction mixture of MF and a compound of Formula (7) can be filtered prior to heating. The filtration step can remove any insoluble material (e.g., insoluble salts) that remain in the reaction mixture. In some embodiments, the solvent can be removed from the filtrate prior to 25 heating (i.e., the residue can be heated neat). 8 In further embodiments, the nonpolar solution of the reaction mixture of MF and a compound of Formula (7) can be filtered prior to heating, the nonpolar solvent can be removed (e.g., by evaporation), and the heating of the sample can be performed in a different solvent. 5 In the methods described above, Y can be any leaving group, for example, Y can be, for example, triflate, mcsylatc, nonaflate, hexaflatc, tosylate, nosylatc, brosylate, perfluoroalkyl sulfonate, tetraphenylborate, hexafluorophosphate, trifluoroacetate, tetrafluoroborate, perchlorate, perfluoroalkylcarboxylate, chloride, bromide, or iodide. 10 M can vary depending on the nature of the X moicty. In some embodiments, M can be potassium, sodium, cesium, complexes of lithium, sodium, potassium, or cesium with cryptands or crown ethers, tetrasubstituted ammonium cations, or phosphonium cations. The nonpolar solvent used in the methods described herein can be, for 15 example, benzene, toluene, o-xylene, m-xylene, p-xylene, ethyl benzene, carbon tetrachloride, hexane, cyclohexane, fluorobenzene, chlorobenzene, nitrobenzene, or mixtures thereof In some embodiments, the nonpolar solvent comprises benzene. In some embodiments, the nonpolar solvent comprises toluene. The polar solvent used in the methods described herein can be, for example, 20 acetonitrile, acetone, dichloromethane, ethyl acetate, tetrahydrofuran, dimethylformamide, 1,2-difluorobenzene, benzotrifluoride or mixtures thereof. Heating of the reaction mixture can include heating at a temperature ranging from about 25* C to about 2500 C. In some embodiments, the heating can occur for from about I second to about 25 minutes. In some embodiments, the heating is 25 accomplished by a flash pyrolysis method, a conventional heating method, or by a microwave method. 9 In some embodiments, the compound of Formula (2) is chosen from: 11N P 1,N P 2 1N'2 P N N' Y O1p5 Osp 0, Ar O Ar 1 0 0 OP3 Ar AI Op 4 Op 4 y Op 4

PNN'P

1 Os Op Ar 1

N

0 O I,'Ar op 3 op 3 p 3 P3 Y 1 Arl wherein each of P'and p 2 are independently a nitrogen protecting group, or P and P 2 come together to form a single nitrogen protecting group; each of P 3 , and P 4 are 5 independently an alcohol protecting group, or P 3 and P 4 come together to form a single oxygen protecting group; and P 5 is a carboxylic acid protecting group. For example, the compound of Formula (2) can be: P N Y OP Ar" O OP3 op 4 wherein each of P'and P 2 are independently a nitrogen protecting group, or P1 and P 2 10 come together to form a single nitrogen protecting group; each of P3, and P 4 are independently an alcohol protecting group, or P 3 and P 4 come together to form a single oxygen protecting group; and P 5 is a carboxylic acid protecting group. In some embodiments, the compound of Formula (2) can be: 0 0 t-Bu,O N 0-k.t-Bu Ar O NOMe OMe 10 In some embodiments, the compound of Formula (2) can be: 0 0 MeO tt-Bu,0 N kOt-Bu Tf 0 OMe MeO In some embodiments, the compound of Formula (2) is chosen from: y NY N N IAr1 ArA Ar CN CN CN y y -N/y N K 1 N KK N Kr N Ari Ar CN CN CN 5 In some embodiments, the compound of Formula (2) is chosen from: Op 3 op 3 Arl'N Y4- AN6t p 4 -o wherein each of P 3 and P 4 are independently an alcohol protecting group. 11 In some embodiments, the compound of Formula (1) or Formula (3) is chosen from: PN PN'p 2 P1 N2 OsPS Osp5 Op5 F N 0 F 0 0 OP3 F A

OP

4

OP

4

OP

4 p1 N'2 N-P2 N Op5

O

0 p5 p5 0 F p

AOP

3 OP 3 F wherein each of P'and P 2 are independently a nitrogen protecting group, or P1 and P 2 5 come together to form a single nitrogen protecting group; each of P 3 , and P 4 are independently an alcohol protecting group, or P 3 and P 4 come together to form a single oxygen protecting group; and Ps is a carboxylic acid protecting group. In some embodiments, the compound of Formula (1) or Formula (3) is chosen from: N C N F NN F F ON ON ON -<" IS -N N N 10 CN CN CN 12 In some embodiments, the compound of Formula (1) or Formula (3) is chosen from:

OP

3

OP

3 F p 4 -O A F P4-O wherein each of p 3 and P 4 are independently an alcohol protecting group. 5 In some embodiments, the compound of Formula (1) or Formula (3) can be:

P

1

,N'

2 OP5 F- 0% F 0

OP

4 wherein each of P'and P 2 are independently a nitrogen protecting group, or P1 and P 2 come together to form a single nitrogen protecting group; each of P3, and P 4 are independently an alcohol protecting group, or P3 and P 4 come together to form a 10 single oxygen protecting group; and P 5 is a carboxylic acid protecting group. For example, the compound of Formula (1) or Formula (3) can be: 0 0 t-Bu,O N 0-t-Bu 0NOs F O NOMe OMe In some embodiments, the compound of Formula (1) or Formula (3) can be:

NH

2 OH F 0 OH HO 15 13 In some embodiments, the compound of Formula (7) can be: 0 0 t-BuO N. 0-.At-Bu Ar A OMe OMe For example, the compound of Formula (7) can be: 0 0 t-Bu,'O NJ O-t-Bu MeO TfO OMe MeO 5 In some embodiments, the compound of Formula (6) can be: 0 0 0 NO t-BusO0 N k0-t-Bu F O OMe OMe Also provided herein is a method for making a compound of Formula (1) that can include heating a mixture comprising a nonpolar solvent and a compound of Formula (5): x Ari-I' 10 5 wherein Ar is an electron rich aryl or heteroaryl ring system; and Ar 2 and X are as defined for Formula (1). In some embodiments, the reaction mixture is filtered (i.e., to remove insoluble material) prior to heating. In some embodiments, the reaction 15 mixture is filtered and the nonpoloar solvent is removed and the resulting residue is dissolved in a polar solvent prior to heating. In some embodiments, X is F (e.g., "F). 14 Also provided herein is a method for making a compound of Formula (3) that can include heating a mixture comprising a nonpolar solvent and a compound of Formula (4): F Ar1--I Ar2 5 4 wherein Ar is an electron rich aryl or heteroaryl ring system; and Ar 2 is as defined for Formula (3). In some embodiments, the reaction mixture is filtered (i.e., to remove insoluble material) prior to heating. In some embodiments, the reaction mixture is filtered and the nonpoloar solvent is removed and the resulting residue is dissolved in 10 a polar solvent prior to heating. Further provided herein is a compound of Formula (8): PN' F Op5 Arj~

OP

3 Op 4 8 wherein Ar' is an electron rich aryl or heteroaryl ring system; each of Pland P 2 are 15 independently a nitrogen protecting group, or P1 and P 2 come together to form a single nitrogen protecting group; each of P 3 , and P 4 are independently an alcohol protecting group, or P 3 and P 4 come together to form a single oxygen protecting group; and

P

5 is a carboxylic acid protecting group. In some embodiments, the compound of 20 Formula (8) is: 0 0 F <O AtB'O N 0-tB Ar O OMe OMe 15 In some embodiments, the compound of Formula (8) is: 0 0 t-Bu ON) t-Bu - MeO .N OMe MeO A compound of Formula (6) is also provided. The compound can be prepared using any of the methods described herein. 5 The details of one or more embodiments of the invention are set forth in the accompanying drawings and the description below. Other features, objects, and advantages of the invention will be apparent from the description and drawings, and from the claims. DESCRIPTION OF DRAWINGS 10 FIG. 1 shows the decomposition of MTEB-I-F in acetonitrile at 90 *C. FIG. 2 shows the decomposition of MTEB-I-F in benzene at 90 *C. FIG. 3 details the 'H NMR of 6-Fhuoro-L-DOPA FIG. 4 details the 1 9 F NMR of 6-Fluoro-L-DOPA. DETAILED DESCRIPTION 15 Definitions Unless defined otherwise, all technical and scientific terms used herein have the same meaning as is commonly understood by one of ordinary skill in the art to which this disclosure belongs. All patents, applications, published applications, and other publications are incorporated by reference in their entirety. In the event that 20 there is a plurality of definitions for a term herein, those in this section prevail unless stated otherwise. As used herein, the singular forms "a," "an," and "the" include plural referents unless the context clearly dictates otherwise. In general, the term "aryl" includes groups having 5 to 14 carbon atoms which 25 form a ring structure and have an aromatic character, including 5- and 6-membered single-ring aromatic groups, such as benzene and phenyl. Furthermore, the term "aryl" includes polycyclic aryl groups, e.g., tricyclic, bicyclic, such as naphthalene and anthracene. 16 The term "heteroaryl" includes groups having 5 to 14 atoms which form a ring structure and have an aromatic character, including 5- and 6-membered single-ring aromatic groups, that have from one to four heteroatoms, for example, pyrrole, furan, thiophene, thiazole, isothiaozole, imidazole, triazole, tetrazole, pyrazole, oxazole, 5 isooxazole, pyridine, pyrazine, pyridazine, and pyrimidine, and the like. Furthermore, the term "heteroaryl" includes polycyclic heteroaryl groups, e.g., tricyclic, bicyclic, such as benzoxazole, benzodioxazole, benzothiazole, benzoimidazole, benzothiophene, methylenedioxyphenyl, quinoline, isoquinoline, napthridine, indole, benzofuran, purine, benzofuran, deazapurine, indazole, or indolizine. 10 The term "substituted" means that an atom or group of atoms formally replaces hydrogen as a "substituent" attached to another group. For aryl and heteroaryl groups, the term "substituted", unless otherwise indicated, refers to any level of substitution, namely mono, di, tri, tetra, or penta substitution, where such substitution is permitted. The substituents are independently selected, and 15 substitution may be at any chemically accessible position. The compounds provided herein may encompass various stereochemical forms and tautomers. The compounds also encompasses diastereomers as well as optical isomers, e.g. mixtures of enantiomers including racemic mixtures, as well as individual enantiomers and diastereomers, which arise as a consequence of structural 20 asymmetry in certain compounds. Separation of the individual isomers or selective synthesis of the individual isomers is accomplished by application of various methods which are well known to practitioners in the art. The term "electron rich", as used herein, refers to an aryl or heteroaryl ring system which is more easily oxidized than benzene. For example the aryl or 25 heteroaryl ring system may be substituted with one or more substituents having a Hamnett a value of less than zero. The term "fluorine" unless explicitly stated otherwise includes all fluorine isotopes. Multiple fluorine isotopes are known, however, only 19F is stable. The radioisotope ' 8 F has a half-life of 109.8 minutes and emits positrons during 30 radioactive decay. The relative amount of 1 8 F present at a designated site in a compound of this disclosure will depend upon a number of factors including the isotopic purity of 1 8 F labeled reagents used to make the compound, the efficiency of incorporation of 18F in the various synthesis steps used to prepare the compound, and the length of time since the ' 8 F has been produced. When a position is designated 35 specifically as 18F in the methods and compounds of the present disclosure, the 17 position is understood to have at least about 0.01%, at least about 0.1%, at least about 1%, at least about 2%, at least about 3%, at least about 4%, at least about 5%, at least about 10%, at least about 15%, at least about 20%, at least about 25%, at least about 30%, at least about 35%, at least about 45%, at least about 50%, at least about 55%, at 5 least about 60%, at least about 65%, at least about 70%, at least about 75%, at least about 80%, or at least about 85% ' 8 F incorporation at that site. Methods of Preparing Substituted Aryl and Heteroaryl Ring Systems Provided herein are methods of preparing substituted aryl and heteroaryl ring 10 systems using diaryliodonium compounds and intermediates. For example, diaryliodonium salts and diaryliodonium fluorides, as provided herein, can undergo decomposition to prepare an aryl fluoride. For example, provided herein is a method for making a compound of Formula (1): Ar 2 -X 15 wherein Ar 2 is an aryl or heteroaryl ring system; and X is a moiety wherein the pKa of the acid H-X is less than 12. In some embodiments, a compound of Formula (1) can be prepared as shown in Scheme 1. 20 Scheme 1. Y X MX Ari-1-Ar2 MX - Ari-1-Ar2 X-Ar2 2 1 In some embodiments, the method can include reacting in a polar solvent a compound MX, wherein M is a counter ion and X is as defined in Formula (1), and a compound of Formula (2): Y Ar-I 25 Ar2 2 wherein Ar is an electron rich aryl or heteroaryl ring system; Y is a leaving group; and Ar 2 and X are as defined above in Formula (1). The polar solvent can then be removed from the reaction mixture. The remaining mixture can then be combined 30 with a nonpolar solvent and heated to produce a compound of Formula (1). In some embodiments, the method can include heating a mixture comprising a nonpolar solvent, a compound MX, and a compound of Formula (2). 18 In some embodiments, the nonpolar solution of the reaction mixture of MX and a compound of Formula (2) can be filtered prior to heating. The filtration step can remove any insoluble material (e.g., insoluble salts) that remain in the reaction mixture. In some embodiments, the solvent can be removed from the filtrate prior to 5 heating (i.e., the residue can be heated neat). In further embodiments, the nonpolar solution of the reaction mixture of MX and a compound of Formula (2) can be filtered prior to heating, the nonpolar solvent can be removed (e.g., by evaporation), and the heating of the sample can be performed in a different solvent. 10 Substituted aryls and heteroaryls which arc prepared using the methods described herein can have an X moiety which includes any moiety in which the pKa of H-X (i.e., the conjugate acid of X) is less than about 12. Tn some cases, X is a radioactive isotope (e.g., 18 F, 1oI, m'I, and compounds having 32 P and 33 P). In some embodiments, X can be chosen from halide, aryl carboxylate, alkyl carboxylate, 15 phosphate, phosphonate, phosphonite, azide, thiocyanate, cyanate, phenoxide, triflate, trifluoroethoxide, thiolates, and stabilized enolates. For example, X can be fluoride, chloride, bromide, iodide, trifluoroacetate, benzoate, and acetate. In some embodiments, X is fluoride. In some embodiments, is a radioactive isotope of fluoride (e.g., 18 F). 20 Y can be any suitable leaving group. In some embodiments, Y is a weakly coordinating anion (i.e., an anion that coordinates only weakly with iodine). For example, Y can be the conjugate base of a strong acid, for example, any anion for which the pKa of the conjugate acid (H-Y) is less than about 1. For example, Y can be triflate, mesylate, nonaflate, hexaflate, toluene sulfonate (tosylate), nitrophenyl 25 sulfonate (nosylate), bromophenyl sulfonate (brosylate), perfluoroalkyl sulfonate (e.g., pcrfluoro C 2 1 o alkyl sulfonate), tetraphenylborate, hexafluorophosphate, trifluoroacetate, perfluoroalkylcarboxylate, tetrafluoroborate, perchlorate, hexafluorostibate, hexachlorostibate, chloride, bromide, or iodide. In some embodiments, a slightly more basic leaving group such as acetate or benzoate may be 30 used. The counter ion M can be any suitable cation for the desired X. The choice of the source of X, and accordingly M, is readily within the knowledge of one of ordinary skill in the art. For example, M can be chosen from an alkali metal, alkaline earth metal and transition metal salts such as, for example, calcium, magnesium, 35 potassium, sodium and zinc salts. Metal cations may also be complexed to cryptands 19 or crown ethers to enhance their solubility and to labilize the X moiety. M can also include organic salts made from quaternized amines derived from, for example, NN' dibenzylethylenediamine, chloroprocaine, choline, diethanolamine, ethylenediamine, meglumine (N-methylglucamine) and procaine. In some embodiments, M can be a 5 lithium, sodium, potassium, or cesium with cryptands or crown ethers, a tetrasubstituted ammonium cation, or phosphonium cation. When X is fluoride, the choice of fluoride source is also readily within the knowledge of one of ordinary skill in the art. A variety of fluoride sources can be used in the preparation of the fluorinated aryl and heteroaryl compounds as provided herein, including but not 10 limited to NaF, KF, CsF, tetrabutylammonium fluoride, and tetramothylammonium fluoride. In certain instances the choice of fluoride source will depend on the functionality present on the compound of Formula (2). The methods described above can be useful in the preparation of fluorinated aryl and heteroaryl ring systems. For example, the methods can be used to prepare a 15 compound of Formula (3): Ar 2 -F 3 wherein Ar2 is an aryl or heteroaryl ring system. In particular, the methods can be used to prepare radiolabeled fluorinated aryl and heteroaryl ring systems (e.g., PET 20 radiotracers). In some embodiments, the method can include reacting in a polar solvent a compound MF and a compound of Formula (2). The polar solvent can then be removed from the reaction mixture. The remaining mixture can then be combined with a nonpolar solvent and heated to produce a compound of Formula (3). In some embodiments, the method can include heating a mixture comprising a 25 nonpolar solvent, a compound MF, and a compound of Formula (2). In some embodiments, the nonpolar solution of the reaction mixture of MF and a compound of Formula (2) can be filtered prior to heating. The filtration step can remove any insoluble material (e.g., insoluble salts) that remain in the reaction mixture. In some embodiments, the solvent can be removed from the filtrate prior to 30 heating (i.e., the residue can be heated neat). In some embodiments, the nonpolar solution of the reaction mixture of MF and a compound of Formula (2) can be filtered prior to heating, the nonpolar solvent can be removed (e.g., by evaporation), and the heating of the sample can be performed in a different solvent. 20 In some embodiments, the compound of Formula (3) can be a compound of Formula (6): N F N 0

OP

3 op 4 6 5 wherein each of Pland P 2 are independently a nitrogen protecting group, or P 1 and P 2 come together to form a single nitrogen protecting group; each of p3, and P 4 are independently an alcohol protecting group, or P 3 and P 4 come together to form a single oxygen protecting group; and P 5 is a carboxylic acid protecting group. In some embodiments, the method can include reacting in a polar solvent a compound MF and 10 a compound of Formula (7): P N Y O1p5 Ar 0

OP

3

OP

4 7 wherein Ar' is an electron rich aryl or heteroaryl ring system; Y is a leaving group; and P',P 2 p 3 p 4 and P 5 are as defined in Formula (6). The polar solvent can then be 15 removed from the reaction mixture. The remaining mixture can then be combined with a nonpolar solvent and heated to produce a compound of Formula (6). In some embodiments, the method can include heating a mixture comprising a nonpolar solvent, a compound MF, and a compound of Formula (7). In some embodiments, the nonpolar solution of the reaction mixture of MF 20 and a compound of Formula (7) can be filtered prior to heating. The filtration step can remove any insoluble material (e.g., insoluble salts) that remain in the reaction mixture. In some embodiments, the solvent can be removed from the filtrate prior to heating (i.e., the residue can be heated neat). 21 The compound of Formula (6) can be, for example, P N' F O Op 3 op 4 In some embodiments, the compound of Formula (6) is: 0 0 t-Bu 0 N k 0-t-Bu O N F OMe 5 Accordingly, the compound of Formula (7) can be, for example: P1N' Y OsP5 Ary 0 OP3 op 4 In some embodiments, the compound of Formula (7) can be: 0 0 t-Bu,. Ok k 0t-Bu 0 O Arl NOMe OMe In some embodiments, the compound of Formula (7) can be: 0 0 MO t-Bu'O N 10-t-Bu MeOO 0 Tfd O OMe 10 MeO The moiety Arl can be an electron-rich aryl or heteroaryl ring system. For example, in some embodiments, Ar'-H is more easily oxidized than benzene. In 22 some embodiments, Art' can be substituted with at least one substituent having a Hammett op value of less than zero (see, for example, "A survey of Hammett substituent constants and resonance and field parameters", Corwin. Hansch, A. Leo, R. W. Taft Chem. Rev., 1991, 91 (2), pp 165-195). For example, Ar' can be 5 substituted with at least one of -(C-Cio)alkyl, -(C-Co)haloalkyl,

(C

2 -Cio)alkenyl,

(C

2 -Cio)alkynyl, -O-(C-Cio)alkyl, -C(O)-O-(C-Co)alkyl, aryl, and heteroaryl. In some embodiments, Ar' is: R2 R1 R3 R4 R5 wherein R', R2, RW, R4, and RW are independently chosen from: H, -(C1-Cio)alkyl, -(C 10 Cio)haloalkyl, (C 2 -Cio)alkenyl, (C 2 -Cio)alkynyl, -O-(C-Cto)alkyl, -C(O)-O-(C Cio)alkyl, aryl, and heteroaryl, or two or more of R1, R 2 , RW, R 4 , and R 5 come together to form a fused aryl or heteroaryl ring system. In some embodiments, Ar' is the same as Ar2. In some embodiments, Art' is more easily oxidized than Ar2. 15 In some embodiments, Ar' can be substituted with a solid support. A "solid support" may be any suitable solid-phase support which is insoluble in any solvents to be used in the process but which can be covalently bound (e.g., to Art' or to an optional linker). Examples of suitable solid supports include polymers such as polystyrene (which may be block grafted, for example with polyethylene glycol), 20 polyacrylamide, or polypropylene or glass or silicon coated with such a polymer. The solid support may be in the form of small discrete particles such as beads or pins, or as a coating on the inner surface of a reaction vessel, for example a cartridge or a microfabricated vessel. See, for example, U.S. Patent Application No. 2007/0092441. In some embodiments, the solid support is covalently bound to Ar' through the 25 use of a tinker. A "linker" can be any suitable organic group which serves to space the Art' from the solid support structure so as to maximize reactivity. For example, a linker can include a C1- 2 0 alkyl or a C1- 2 o alkoxy, attached to the solid support, for example, a resin by an amide ether or a sulphonamide bond for ease of synthesis. The linker may also be a polyethylene glycol (PEG) linker. Examples of such linkers are 30 well known to those skilled in the art of solid-phase chemistry. The methods described herein can be used with a variety of aryl and heteroaryl ring systems. As is well understood by one of skill in the art, to carry out efficient 23 nucleophilic substitution of the aryl and heteroaryl ring systems described herein, it is necessary that Ar' be more easily oxidized (i.e., more electron rich) than Ar. Within that boundary, however, the Ar2 moiety can be any aryl or heteroaryl ring system in which substitution by X (e.g., F such as l8F) is desired. For example, Ar 2 can be a 5 phenylalanine, tyrosine, typtophan, or histidine derivative, and an estradiol derivative. In some embodiments, Ar 2 can be chosen from: OMe CN MeO Me Me OM e OMe N N CF 3 24 PIN N'p,1 'P 2 PI I Pt2 - W o 0 0,N

OP

4

OP

4

OP

4 pI I 'P 2 Pt I NP 2 Pt N 2 '0 p5 0 P5 0>5 PI 'P I N N 2 0 P N 0'P Zaps A lp A l

'/

0 0

N

0 Pt, N'2 Pt 2 N N

P

6 p 6 25 pl. NP 2 p1. INp2 P 1 . I 'P 2 N N o 0 pp oi 4 N .76 N, plt N'2 Pt 'N'P 2 PIIt ~p 2 o 0 0 NN 'N Kl N' 2 Pt INp 2 P I NP 2 N N N N, P 'E ' 'P6 N N P 6 'p,6 26 P1, N p 2 p1 I 'p 2 P1 N' 2 N N N'

P

3 N p 3 p 3 N- 0 06IL N '6N P \p OP / 3 Op P

P

1 N N N' p7~ p70P-OpO Np3 p3 - pp3 p3 PsNN N OP4 CN N CN CN C CN Op3 CN wherein each of PI, p2 and p6 are independently a nitrogen protecting group, or P and P2 come together to form a single nitrogen protecting group;, and each of P', P4, P' and P7 are independently an oxygen protecting group, or p3 and p4 Come together to form 27 a single oxygen protecting group. In some embodiments, Ar 2 is an electron rich aryl or heteroaryl ring system. Protecting groups can be a temporary substituent which protects a potentially reactive functional group from undesired chemical transformations. The choice of the 5 particular protecting group employed is well within the skill of one of ordinary skill in the art. A number of considerations can determine the choice of protecting group including, but not limited to, the functional group being protected, other functionality present in the molecule, reaction conditions at each step of the synthetic sequence, other protecting groups present in the molecule, functional group tolerance to 10 conditions required to remove the protecting group, and reaction conditions for the thermal decomposition of the compounds provided herein. The field of protecting group chemistry has been reviewed (Greene, T. W.; Wuts, P. G. M. Protective Groups in Organic Synthesis, 2.sup.nd ed.; Wiley: New York, 1991). A nitrogen protecting group can be any temporary substituent which protects 15 an amine moiety from undesired chemical transformations. Examples of such protecting groups include, but are not limited to allylamine, benzylamines (e.g., bezylamine, p-methoxybenzylamine, 2,4-dimethoxybenzylamine, and tritylamine), acetylamide, trichloroacetammide, trifluoroacetamide, pcnt-4-enamide, phthalimides, carbamates (e.g., methyl carbamate, t-butyl carbamate, benzyl carbamate, allyl 20 carbamates, 2,2,2-trichloroethyl carbamate, and 9-fluorenylmethyl carbamate), imines, and sulfonamides (e.g., benzene sulfonamide, p-toluenesulfonamide, and p nitrobcnzenesulfonamide). An oxygen protecting group can be any temporary substituent which protects a hydroxyl moiety from undesired chemical transformations. Examples of such 25 protecting groups include, but are not limited to esters (e.g., acetyl, t-butyl carbonyl, and benzoyl), benzyl (e.g., benzyl, p-methoxybenzyl, and 2,4-dimethoxybenzyl, and trityl), carbonates (e.g., methyl carbonate, allyl carbonate, 2,2,2-trichloroethyl carbonate and benzyl carbonate) ketals, and acetals, and ethers. 28 In some embodiments, a compound of Formula (2), as provided herein, can be chosen from:

PIN

2 N N'2 y OIpS y 0 'pS O'p5 Arl' 0 Arl' 0 0 -3 Ar A Op 4 Op 4 y p4 PtN' N' N'p 1 Ar ON0 NOArl Ar OP 3

OP

3 OP3 Y 'Ar 5 wherein: each of P1and P 2 are independently a nitrogen protecting group, or P' and P 2 come together to form a single nitrogen protecting group; each of P 3 and P 4 are independently an oxygen protecting group, or P 3 and P 4 come together to form a single oxygen protecting group, and P 5 is a carboxylic acid 10 protecting group. For example, a compound of Formula (2) can be: PN' Y OIp5 ArI O OP3 op 4 In some embodiments, a compound of Formula (2) can be: 0 0 t-Bu,'O N 0-t-Bu Arl N OMe OMe 29 In some embodiments, a compound of Formula (2) can be: 0 0 t-BuO [ O-t-Bu MeO = O Tfd OMe MeO In some embodiments, a compound of Formula (2) is chosen from: 9 y --N N N N N N~~ IA N Nl Y I Ar AAr Ar CN CN CN S/ N ON CN CN 5 In other embodiments, a compound of Formula (2) is chosen from: op 3 OP 3 Y p4- O A rF I Y Ar1 wherein: each of P 3 and P 4 are independently an alcohol protecting group. 10 30 In some embodiments, a compound of Formula (1) or Formula (3) can be chosen from: plt N' 2 P, N'p 2 p 1 ,N P2 PN' N N' 0,, Op 6 O~p F O F 0 O O OP3 F Op 4

OP

4

OP

4 K~p p 2 Ptp p N N'p2 N' F 0 0 0 F Op 3

OP

3 F wherein each of P'and P 2 are independently a nitrogen protecting group, or P 1 and P 2 5 come together to form a single nitrogen protecting group; and each of P 3 and p 4 are independently an alcohol protecting group, or P 3 and P 4 come together to form a single oxygen protecting group, and P 5 is a carboxylic acid protecting group. For examples, a compound of Formula (1) or Formula (3) can be: P1N' F N 0 OP3 10 In some embodiments, a compound of Formula (1) or Formula (3) can be: 0 0 t-Bu% AkN .- t-Bu ON FO OMe OMe 31 In some embodiments, a compound of Formula (1) or Formula (3) can be:

NH

2 OH F 0 OH HO In some embodiments, a compound of Formula (1) or Formula (3) can be chosen from: N NI F F F CN CN CN S S 41 -N N >F N >F F 5 CN CN CN In some embodiments, a compound of Formula (1) or Formula (3) is chosen from:

OP

3

OP

3 F P4-O0 10 F P 4 -0 wherein each of P 3 and P 4 are independently an alcohol protecting group. A nonpolar solvent can be any solvent having a dielectric constant of less than about 10. For example, a nonpolar solvent can be chosen from benzene, toluene, o xylene, m-xylene, p-xylene, ethyl benzene, carbon tetrachloride, hexane, cyclohexane, 15 fluorobenzene, chlorobenzene, nitrobenzene, and mixtures thereof In some embodiments, the nonpolar solvent comprises benzene. In some embodiments, the nonpolar solvent comprises toluene. In some embodiments, the nonpolar solvent 32 comprises cyclohexane. In some embodiments the nonpolar solvent is a mixture, for example a mixture of cyclohexane and toluene. A polar solvent is a solvent having a dielectric constant greater than about 10. In some embodiments, the polar solvent is a polar aprotic solvent, such as acetonitrile, 5 acetone, dichloromethane, ethyl acetate, tetrahydrofuran, dimethylformamide, 1,2 difluorobenzene, bcnzotrifluoride, and mixtures thereof. In some embodiments, the polar aprotic solvent is acetonitrile. Heating can be accomplished by conventional means (e.g., heating bath, oven, heat gun, hot plate, Bunsen burner, heating mantle, and the like), by the use of a 10 microwave, or by flash pyrolysis. Typically, the reaction mixture is heated at a temperature ranging from about 250 C to about 2500 C (e.g., between about 80' C to about 2000 C, 100* C to about 2000 C, about 1200 C to about 1700 C, about 1200 C to about 1600 C, about 1200 C to about 1500 C, and about 1300 C to about 150 C). In some embodiments, the reaction mixture is heated to about 140 0 C. Heating can 15 occur for any time necessary to complete the reaction. For example, heating can occur for from about 1 second to about 25 minutes (e.g., about 2 seconds, about 5 seconds, about 10 seconds, about 30 seconds, about 1 minute, about 90 seconds, about 2 minutes, about 3 minutes, about 5 minutes, about 8 minutes, about 10 minutes, about 12 minutes, about 15 minutes, about 20 minutes, and about 24 minutes). In 20 some embodiments, heating can occur for from about I second to about 15 minutes. Further provided herein is a method of making a compound of Formula (1) that includes heating a mixture comprising a nonpolar solvent and a compound of Formula (5): X Ar-/ Ar2 25 5 wherein Ar' is an electron rich aryl or heteroaryl ring system; and Ar 2 and X are as defined for Formula (1). In some embodiments, the method can include filtering the mixture prior to heating. Filtering, as described above, can remove insoluble materials such as insoluble salts. In another embodiment, the method can include, 30 prior to heating, filtering the mixture, removing the nonpolar solvent, and subsequently heating a solution of the remaining reaction mixture and a polar solvent. As described above, the methods described herein can be used to prepare fluorinated (e.g., 18F) aryl and heteroaryl ring systems. Accordingly, further provided 33 herein is a method for making a compound of Formula (3) that includes heating a mixture comprising a nonpolar solvent and a compound of Formula (4): F Ar 1 -I Ar 2 4 5 wherein Ar' is an electron rich aryl or heteroaryl ring system; and Ar 2 is as defined for Formula (3). In some embodiments, the method can include filtering the mixture prior to heating. Filtering, as described above, can remove insoluble materials such as insoluble salts. In another embodiment, the method can include, prior to heating, filtering the mixture, removing the nonpolar solvent, and subsequently heating a 10 solution of the remaining reaction mixture and a polar solvent. In the methods described herein, a pressure tube or other reinforced closed system can be used in instances where the desired temperature is above the boiling point of the solvent utilized. The reaction can be conducted in the presence of an inert gas such as nitrogen 15 or argon. In some embodiments, steps are taken to remove oxygen and/or water from the reaction solvent and starting materials. This can be accomplished by a number of methods including distillation of solvents in the presence of agents that react with and/or sequester water and under an atmosphere of inert gas; and purging the reaction vessel with an inert gas. 20 The methods described herein can be used when MX (e.g., MF) is reacted in an amount ranging from about 1 picomole to about 10 millimoles (e.g., about I picomole to about 5 millimoles; about 1 picomole to about 1 nillimole; about 1 picomole to about 500 micromoles; about 1 picomole to about 100 micromoles; about I picomole to about 50 micromoles; about I picomole to about 5 micromoles; about 1 25 picomole to about 1 micromole; about 1 picomole to about 500 nanomoles; about 1 picomole to about 100 nanomoles; about 1 picomole to about 50 nanomoles; about 1 picomole to about 5 nanomoles; about 1 picomole to about 1 nanomole; about 100 picomoles to about 10 millimoles; about 500 picomoles to about 10 millimoles; about I nanomole to about 10 millimoles; about 50 nanomoles to about 10 millimoles; about 30 100 nanomoles to about 10 millimoles; about 500 nanomoles to about 10 millirnoles; about 1 micromole to about 10 millimoles; about 50 micromoles to about 10 millimoles; about 100 micromoles to about 10 millimoles; about 500 micromoles to about 10 millimoles and about 1 millimole to about 10 millimoles). In some embodiments, MX is reacted in the sample in an amount of less than about 10 34 millimoles. In many cases, the compound of Formula (2) is used in an excess when compared to the amount of MX present in the sample. In some embodiments, the reaction mixture having MIX further contains additional compounds which may be present in an excess compared to MX. For example, the additional compounds may 5 be present in more than one million fold excess compared to MX. Compounds Diaryliodonium compounds, for example, compound of Formula (2), (4), (7) and (8), are further provided herein. For example, a compound of Formula (8) is 10 provided, P1N' F Oy Ar 0 OP3

OP

4 8 wherein Ar' is an electron rich aryl or heteroaryl ring system; each of Pland P 2 are independently a nitrogen protecting group, or P 1 and P 2 come together to form a 15 single nitrogen protecting group; each of P 3 , and P 4 are independently an alcohol protecting group, or P3 and P 4 come together to form a single oxygen protecting group; and P 5 is a carboxylie acid protecting group. In some embodiments, the compound of Formula (8) can be: 0 0 t-Bu'O N k0-t-Bu F O Ar O OMe OMe 20 In some embodiments, a compound of Formula (8) can be: 0 0 MeO O t-Bu ' kO-t-Bu MeO 0O OMe MeO 35 The diaryliodonium compounds of Formula (2), (4) and (7) can be prepared from commercially available starting materials using various methods known to those of ordinary skill in the art. The method used for synthesizing the compounds will depend on the electronics and functionality present in of Ar 2 . Potentially reactive 5 functional groups present in Ar2 can be masked using a protecting group prior to the synthesis of the diaryliodonium compound. The particular method employed for preparing the diaryliodonium compounds will be readily apparent to a person of ordinary skill in the art. For example, the compounds can be made using the following generic reactions as shown in Scheme 2. 10 Scheme 2. Y Y Ar 1 -I + Ar 2 -H Ar 1 -I-Ar 2 + HY conditions Y Y Arl-H + Ar2- Ari-1-Ar2 + HY I conditions Y Y Ar 1 -I + Ar 2 -M Ar 1 -i-Ar 2 + MY conditions Y Y Ari-M + Ar 2 -I - Ar 1 -I-Ar 2 + MY I conditions Y For compounds that bear sensitive functionality on the accepting group, organometallic reagents that feature more covalent (more stable) C-M bonds can be used. For example, organometallic compounds including tin, boron, and zinc. If there 15 is no functional group incompatibility, more basic organometallic reagents (organolithium, Grignard, etc.) can be used to prepare the diaryliodonium salts. Persons skilled in the art will be aware of variations of, and alternatives to, the processes described which allow the compounds defined herein to be obtained. It will also be appreciated by persons skilled in the art that, within certain of 20 the processes described, the order of the synthetic steps employed may be varied and will depend inter alia on factors such as the nature of other functional groups present in a particular substrate, the availability of key intermediates, and the protecting group strategy (if any) to be adopted. Clearly, such factors will also influence the choice of reagent for use in the said synthetic steps. 36 The skilled person will appreciate that the diaryliodonium compounds described could be made by methods other than those herein described, by adaptation of the methods herein described and/or adaptation of methods known in the art, for example US 2007/0092441, or using standard textbooks such as "Comprehensive 5 Organic Transformations--A Guide to Functional Group Transformations", R C Larock, Wiley-VCH (1999 or later editions) and Science of Synthesis, Volume 31a, 2007 (Houben-Weyl, Thieme) It is to be understood that the synthetic transformation methods mentioned herein are exemplary only and they may be carried out in various different sequences 10 in order that the desired compounds can be efficiently assembled. The skilled chemist will exercise his judgment and skill as to the most efficient sequence of reactions for synthesis of a given target compound. As exemplified in the examples below, certain diaryliodonium fluorides can be prepared by H 2

SO

4 catalyzed clcetrophilic aromatic substitution of the aromatic 15 fluorine precursor with ArI(OAc) 2 , followed by ion exchange. The desired diaryliodonium fluoride is formed by reacting the resulting diaryliodonium salt with a fluoride source, such as tetrabutylammonium fluoride, as illustrated in Scheme 3 shown below. Scheme 3. 1. Arl(OAc) 2 X Catalytc H 2 SO4 F 2. Ion Exchange TBAF 20 RRC Diaryliodonium fluorides can also be prepared by the reaction of the corresponding tributylstannanyl derivative of the aromatic fluorine precursor with p MeOPhI(OH)(OTs), followed by ion exchange, and reaction of the resulting diaryliodonium salt with a fluoride source, such as tetrabutylammonium fluoride, as 25 illustrated in Scheme 4. Scheme 4. xe F Sn(Bu) 1 p-MeOPhI(OH)(OAc) F 3 2 Ion ExchangehO AF aO!Me R7 W~e The choice of fluoride source is readily within the knowledge of one of ordinary skill in the art. A variety of fluoride sources can be used in the preparation of 30 the diaryliodonium fluorides as provided herein, including but not limited to NaF, KF, 37 CsF, tetrabutylammonium fluoride, and tetramethylammonium fluoride. In certain instances the choice of fluoride source will depend on the functionality present on the aromatic fluoride precursor. Further provided are compounds of Formula (1) and Formula (3) which are 5 prepared by the methods described herein. For example, a compound of Formula (6) is provided, wherein the compound is prepared as described above. EXAMPLES General Methods. 10 Tetramethylammonium fluoride (TMAF, Aldrich) and diphonyliodonium nitrate were dried at 60-80* C in a drying pistol (charged with P 2 0 5 ) under dynamic vacuum for one week. Hexabutylditin and tributyltin chloride (Aldrich) were distilled into flame-dried storage tubes under dry nitrogen. Acetonitrile and acetonitrile-d were refluxed with P 2 0 5 , benzene and benzene-d 6 were refluxed with CaH 2 , overnight 15 and distilled directly into flame-dried storage tubes under dry nitrogen. All glassware, syringes, and NMR tubes were oven dried (1400 C) for more than 24 hours before they were transferred into the glove box for use. All other reagents were purchased from commercial sources and were used as received. All NMR experiments were performed using a Bruker Avance 400 MHz NMR spectrometer. 20 Example I - Preparation ofp-nethoxyphenyliodonium diacetate p-methoxyphenyliodonium diacetate: 2.34 g (10 mmol)p-iodoanisole was dissolved in 90 mL of glacial acetic acid. The solution was stirred, heated to 400 C and 13.6 g (110 mmol) sodium perborate tetrahydrate was added gradually over an 25 hour. The reaction mixture was kept at 40* C for 8 hours before being cooled to room temperature. Half of the acetic acid (-45 mL) was removed and 100 mL of D.I. water was added. 3x4O mL dichloromethane was used to extract the aqueous solution. The combined organic layers were dried over sodium sulfate and solvent was evaporated to give 2.25 g (64%) of p-methoxyiodonium diacetate, which was dried in vacuo and 30 used without further purification. o-methoxyphenyliodonium diacetate (65%), m cyanohenyliodonium diacetate (70%), n-trifluoromethyliodnium diacetate (80%), and 2,6-dimethoxyphenyliodoniu diacetate (83%) were synthesized using a similar procedure from corresponding iodoarenes. 38 Example 2 - Preparation of bis(p-methoxypheny)iodonium trifluoroacetate Bis(p-methoxyphonyl)iodonium trifluoroacetat: Under N 2 protection, 1.41 g (4 mmol)p-methoxyphenyliodonium diacetate was dissolved in 30 mL of dry dichloromethane and the solution was cooled to -30* C. 0.61 mL (8 mmol) of 5 trifluoroacetic acid was added and the solution was slowly brought back to room temperature and stirred for 30 minutes. The solution was, again, cooled to -30* C and 0.44 mL (4 mmol) anisole was added slowly and the mixture was warmed back up to room temperature and stirred for 1 hour. The solvent was evaporated and the residual solid was recrystallized from diethylether/dichloromethane to give 1.53 g bis(p 10 methoxyphcnyl)iodonium trifluoroacetate (71%). Example 3 - Preparation ofBis(p-methoxypheny)iodonium tosylate Bis(p-methoxyphenyl)iodonium tosylate: Under N 2 protection, 352 mg (1 mmol) p-mothoxyphenyliodonium diacetate was dissolved in 1.5 mL of dry 15 acetonitrile. The solution was combined with a solution of 190 mg (1 mmol) tosylic acid monohydrate in 1.5 mL of dry acetonitrile. After addition of 0.11 mL (1 mmol) p-iodoanisole, the mixture was allowed to react at room temperature for 2 hours. The solvent was then removed and the remaining solid was recrystallized from diethylether/dichloromethane to give 422 mg bis(p-methoxyphenyl)iodonium tosylate 20 (82%). Example 4 - Preparation ofBis(p-methoxyphenyl)iodonium hexafluorophosphate Bis(p-methoxyphenyl)iodonium hexafluorophosphate: Under N 2 protection, 352 mg (1 mmol) p-methoxyphenyliodonium diacetate was dissolved in 1.5 mL of 25 dry acetonitrile. The solution was combined with a solution of 190 mg (1 mmol) tosylic acid monohydrato in 1.5 mL of dry acetonitrile. After addition of 0.11 mL (1 mmol) p-iodoanisole, the mixture was allowed to react at room temperature for 2 hours. 10 mL of water was added to the reaction mixture followed by extraction with 3 x5 mL hexanes. The water layer was treated with 502 mg (3 mmol) NaPF 6 .The 30 white precipitation was taken up in dichloromethane and recrystallization with diethylether/dichloromethane provided 391 mg bis(p-methoxyphenyl)iodonium hexafluorophosphate (80.5%). 39 Example 5 - Preparation ofPhenyl-4-methoxyphenyliodonium hexaluorophosphate Phenyl-4-methoxyphenyliodonium hexafluorophosphate was synthesized according to the procedure described for the synthesis of bis(p methoxyphenyl)iodonium hexafluorophosphate from the corresponding aryliodonium 5 diacetate and anisole. (77.9%) Example 6 - Preparation of 2-methoxyphenyl-4 '-methoxyphenyliodonium hexafluorophosphate 2-methoxyphenyl-4'-methoxyphenyliodonium hexafluorophosphate was 10 synthesized according to the procedure described for the synthesis of bis(p methoxyphenyl)iodonium hexafluorophosphate from the corresponding aryliodonium diacetate and anisole. (83.3%) Example 7 - Preparation of3-cyanophenyl-4 '-methoxyphenyliodonium 15 hexafluorophosphate 3-cyanophenyl-4'-methoxyphenyliodonium hexafluorophosphate was synthesized according to the procedure described for the synthesis of bis(p mcthoxyphenyl)iodonium hexafluorophosphate from the corresponding aryliodonium diacetate and anisole. (73.7%) 20 Example 8 - Preparation of 3-(trifluoromethyl)phenyl-4'-methoxyphenyliodoniun hexafluorophosphate 3-(trifiuoromethyl)phenyl-4'-methoxyphenyliodonium hexafluorophosphate was synthesized according to the procedure described for the synthesis of bis(p 25 methoxyphenyl)iodonium hexafluorophosphate from the corresponding aryliodonium diacetate and anisole. (96.1%) Example 9 - Preparation of 2, 6-dimethoxyphenyl-4'-methoxyphenyliodonium hexapluorophosphate 30 2,6-dimcthoxyphonyl-4'-mcthoxyphcnyliodonium hexafluorophosphate was synthesized according to the procedure described for the synthesis of bis(p methoxyphenyl)iodonium hexafluorophosphate from the corresponding aryliodonium diacetate and anisole. (86%) 40 Example 10 - Preparation oJ2-Bromo-4, 5-dimethoxylbenzeneethanamine 2-Bromo-4, 5-dimethoxylbenzencethanamine: Bromine (1.1 mL, 22 mmol) in acetic acid (10 mL) was slowly added into a vigorously stirred solution of 2-(3,4 dimethoxyphenyl)ethylamine (3.4 mL, 20 mmol) in 50 mL acetic acid. 2-bromo-4, 5 5 dimethoxylbenzeneethanamine precipitated out after 15 minutes. The mixture was stirred for another two hours, filtered, and washed with dichloromethane 10 mL x3 and petroleum ether 10 mLx3. The resulting solid was taken up in water and the pH was brought to 10 with aqueous KOH solution. Extraction with dichloromethane followed by evaporation of the solvent yielded 4.12 g (78%) 2-Bromo-4, 5 10 dimethoxylbenzeneethanamine. The crude product was dried under dynamic vacuum overnight and used without further purification. Example II - Preparation of2-Bromo-4, 5-diiethoxyl-(2-phthalimidoethyl)benzene 2-Bromo-4, 5-dimethoxyl-(2-phthalimidoethyl)benzene: 2-Bromo-4, 5 15 dimethoxylbenzeneethanamine (3.5 g 13.2 mmol) was dissolved and stirred in 50 mL dry acetonitrile. 2.14 mL (1.1 equiv) phthaloyl dichloride and 7 mL(3 equiv) Hfinig's base were added. The mixture was stirred at room temperature overnight. Acetonitrile was then removed, and the remaining product was taken up in dichloromethane and washed with basic water (pH=1 1). The aqueous wash was extracted with 20 dichloromethane 3x15 mL. The organic fractions were combined and dried over sodium sulfate. Solvent was removed to give the crude product, which was then purified by column chromatography. Calculated yield: 1.8g (34%). Example 12 - Preparation of 3,4-dimethoxyphenyltributyltin 25 3,4-dimethoxyphenyltributyltin: Under N 2 protection, 1.085 g (5 mmol) 4 bromoveratrole and 289 mg (5 mol%) Pd(0)(PPh 3

)

4 was dissolved in 15 mL of dry toluene, the solution was transferred into a storage tube equipped with a Teflon Chemcap Seal, and 3.19 g (5 mmol) hexabutylditin was added. The tube was sealed, heated to, and kept at 1200 C for 48 hours. The reaction mixture was allowed to cool 30 to room temperature, and diluted with 15 mL hexane. 15 mL of saturated aqueous KF solution was added and the mixture was stirred for 30 minutes followed by filtration through celite. The organic layer was separated; solvent was removed to provide the crude product as a yellow oil. The crude was purified by column chromatography 41 (hexane/dichloromethane 98/2, basic aluminum) to give 1.69 g (79.1%) pure 3,4 dimethoxyphenyltributyltin. Example 13 - Preparation of 3,4-ditnethoxy-2-methylphenyltributyltin 5 3,4-dimethoxy-2-methylphenyltributyltin was synthesized in a similar fashion as described in the procedure for the synthesis of 3,4-dimethoxyphenyltributyltin from the corresponding bromo precursor. (76.2%) Example 14 - Preparation of 3,4-dimethoxy-2-(2-phthaliinido)phenyltributyltin 10 3,4-dimothoxy-2-(2-phthalimido)phcnyltributyltin was synthesized in a similar fashion as described in the procedure for the synthesis of 3,4 dimethoxyphenyltributyltin from the corresponding bromo precursor. (20%) Example 15 - 3,4-dimethoxyphenyl-4 '-methoxyphenyliodonium hexafluorophosphate 15 3,4-dimethoxyphenyl-4'-methoxyphenyliodonium hexafluorophosphate: Under N 2 protection, 352 mg (1 mmol)p-methoxyphenyliodonium diacetate was dissolved in 1.5 mL of dry acetonitrile. The solution was combined with a solution of 190 mg (1 mmol) tosylic acid monohydrate in 1.5 mL of dry aectonitrile. After addition of 427 mg(1 mmol) 3,4-dimethoxyphenyltributyltin, the mixture was 20 allowed to react at room temperature for 2 hours. 10 mL of water was added to the reaction mixture followed by extraction with 3x5 mL hexanes. The water layer was treated with 502 mg (3 mmol) NaPF 6 . The white precipitation was taken up in dichloromethane and recrystallization with diethylether/dichloromethane provided 370 mg (71.7%) 3,4-dimethoxyphenyl-4'-methoxyphenyliodonium 25 hexafluorophosphate. Example 16 - Preparation of 3,4-dimethoxy-2-methylphenyl-4' methoxyphenyliodonium hexafluorophosphate 3,4-dimethoxy-2-methylphenyl-4'-methoxyphenyliodoniurn 30 hexafluorophosphate was synthesized in a similar fashion as 3,4-dimethoxyphenyl-4' methoxyphenyliodonium hexafluorophosphate from p-methoxyphenyliodonium diacetate and the corresponding aryl tin precursor. (75%) 42 Example 17- Preparation of 3,4-dimethoxy-2-(2-phthalimidoethyl)phenyl-4' methoxyphenyliodonium hexafluorophosphate 3,4-dimethoxy-2-(2-phthalimidoethyl)phenyl-4'-methoxyphenyliodonium hexafluorophosphate hexafluorophosphate was synthesized in a similar fashion as 3,4 5 dimethoxyphenyl-4'-methoxyphenyli6donium hexafluorophosphate from p methoxyphenyliodonium diacetate and the corresponding aryl tin precursor. (55%) Example 18 - Preparation of 2-methoxyphenyl-4'-methoxyphenyliodoniunfluoride 2-methoxyphenyl-4'-methoxyphenyliodonium fluoride: Under N 2 protection, 10 97.2 mg (0.2 mmol) 2-methoxyphenyl-4'-methoxyphenyliodonium hexafluorophosphate and 17.7 mg (0.95 equiv) anhydrous tetramethylammonium fluoride (TMAF) were dissolved in I mL dry acetonitrile. The solvent was removed in vacuo followed by addition of 5 mL of dry benzene. The insoluble TMAPF 6 was removed by filtration; the solvent was again removed in vacuo to give 30.3 mg (42%) 15 2-methoxyphenyl-4'-methoxyphenyliodonium fluoride. Example 19 - Preparation ofPhenyl-4-methoxyphenyliodonium fluoride Phenyl-4-methoxyphenyliodonium fluoride was synthesized in a similar fashion as the procedure described for 2-methoxyphenyl-4'-methoxyphenyliodonium 20 fluoride from corresponding hexafluorophosphate. (96%) Example 20 - Preparation of 3-cyanophenyl-4 '-methoxyphenyliodonium fluoride 3-cyanophenyl-4'-methoxyphenyliodonium fluoride was synthesized in a similar fashion as the procedure described for 2-methoxyphenyl-4' 25 methoxyphenyliodonium fluoride from corresponding hexafluorophosphate. (25%) Example 21 - Preparation of 3-(trifluoromethyl)phenyl-4 '-methoxyphenyliodonium fluoride 3-(trifluoromethyl)phenyl-4'-methoxyphenyliodonium fluoride was 30 synthesized in a similar fashion as the procedure described for 2-methoxyphenyl-4' methoxyphenyliodonium fluoride from corresponding hexafluorophosphate. (56%) 43 Example 22 - Preparation of 2,6-dimethoxyphenyl-4'-methoxyphenyliodonium fluoride 2,6-dimethoxyphenyl-4'-methoxyphenyliodonium fluoride was synthesized in a similar fashion as the procedure described for 2-methoxyphenyl-4' 5 methoxyphenyliodonium fluoride from corresponding hexafluorophosphate. (15%) Example 23 - Preparation of 3,4-dimethoxyphenyl-4 '-methoxyphenyliodonium fluoride 3,4-dimethoxyphenyl-4'-methoxyphenyiodonium fluoride was synthesized in 10 a similar fashion as the procedure described for 2-methoxyphonyl-4' methoxyphenyliodonium fluoride from corresponding hexafluorophosphate. (90%) Example 24 - Preparation oJ3,4-ditnethoxy-2-methylphenyl-4' methoxyphenyliodoniumfluoride 15 3,4-dimethoxy-2-methylphenyl-4'-.methoxyphenyliodonium fluoride was synthesized in a similar fashion as the procedure described for 2-methoxyphenyl-4' methoxyphenyliodonium fluoride from corresponding hexafluorophosphate. (80%) Example 25 - Preparation of 3,4-dimethoxy-2-(2-phthalimidoethyl)phenyl-4 20 methoxyphenyliodoniunpfluoride 3,4-dimethoxy-2-(2-phthalimidoethyl)phenyl-4'-methoxyphenyliodonium fluoride was synthesized in a similar fashion as the procedure described for 2 methoxyphenyl-4'-methoxyphenyliodonium fluoride from corresponding hexafluorophosphate. (45%) 25 Example 26 - Preparation of Bis(p-methoxyphenyl)iodoniumfluoride Bis(p-methoxyphenyl)iodonium fluoride: To a mixture of 454 mg (1 mmol) Bis(p-methoxyphenyl)iodonium trifluoroacetate and 262 mg (Immol) anhydrous TBAF was added 1 mL of dry tetrahydrofuran (THF). The solution was allowed to 30 stand for 1 hour, the white precipitate was collected and washed with 3 x0.5 mL THF. Calculated yield: 288.7mg (80.2%) 44 Example 27 - Diaryliodoniumfluoride decomposition In a glove box, 0.5 ml dry d 6 -bcnzcnc was added to 0.02 mmol of the diaryliodonium fluoride, the solution/mixture was transferred to a J-Young NMR tube. The tube was heated to and kept at 140* C for 5 -15 minutes. The resulting 5 solution was analyzed by NMR and GC for product determination. Observed yields of thermal decompositions of the diaryliodonium fluorides prepared above are described in Table 1. 45 Table 1. Yield of total Entry Diaryliodonium fluoride fluoro Yield of ArF Conditions aromatics F 77% (94%) 57%(80%) benzene, __________ ___________140'C, 15min 1770/.o) 47%(70%) acetonile 65%(77%) 40%(70%) 140 0 C, 15min F 99% (94%) 86%* (80%) benzene, 2F- 140'C, 18mm 43%(38%) 43%(38%) 14 0 C, mni n -o 82%(80%) 49%(48%) benzene, 3 140*C, 15min 60%(58%) 40%(38%) 140C, 15mi -o 47%(44%) 19%(17%) benzene, 4 o 140*C, 15min -o 34%(32%) 7%(8%) acetonitrile ___ ______________0_____ 140 0 C, 15min 591%(88%) 77%(74%) 145beizene, 0 1, 140'C, 15min 38%(39%)acetonitrile 38%(39%) 30%(28%) 140 0 C, 15me 690%(92%) 78%(82%0/ ) benzene, / Q- 1 O 140 0 C, 11min 0 81%(78%) 49%(48%) 40Cn 1nnmin F 89% (90%) 89%(90%) benzene, 7 O /\ I - / 140 0 C, 5min \--j acetonitrile CN 78% (77%) 78%(77%) 140'C, 5min 8 95%(92%) 85%(84%) 1404C1 e ien

-

acetonitrile

CF

3 67% (76%) 68%(76%) 140 0 C, 10mm 0- L 80% 9 0 80% (no benzene, N fluoroanisole 140 0 C, 15min 0 Detected) 46 '0 H H. 10 F H4benzene, - o | 5140'C, 15min -0 ( ) determined by GC * benzyne chemistry led to the formation of 3-fluoroanisole Examples 28 - Impact of additional salts on F-MTEB. 5 The effect of salt present in solution during the decomposition of(3-cyano-5 ((2-methylthiazol-4-yl)ethynyl)phenyl)(4-methoxyphenyl)iodonium triflate (Ar MTEB-OTf) was examined at 900 C in benzene and acetonitrile. Each solvent was tested in the absence of salt, presence of I equivalent of salt, and presence of 2 equivalents of salt. The preparation of each reaction condition is summarized below. 10 A TMAF stock solution of 3.3 mg/mL in dry, degassed acetonitrile was prepared for addition to each reaction tube. OTf N N CN Ar-MTEB-OTf 15 Acetonitrile no salt lodonium triflate precursor (0.004 g, 6.6 pmol) was dissolved in 0.38 mL of dry, degassed acetonitrile, under nitrogen atmosphere, with 18 L1 of TMAF (6.6 pmol) stock solution. Next, 0.4 mL of dry, degassed benzene was added to the residue 20 and passed twice through 0.22 pim PTFE membrane filter. The solution was again subjected to vacuum to remove solvent and the remaining residue was dissolved in 0.4 mL of dry, degassed d 3 -acetonitrile. The reaction mixture was placed in a silicon oil bath and monitored at 90 'C. 47 Acetonitrile + 1 eq. TMAOTf Under nitrogen atmosphere, iodonium triflate precursor (0.004 g, 6.6 tamol) was dissolved in 0.38 mL dry, degassed d 3 -acetonitrile, and combined with 18 pL of TMAF (6.6 gmol) stock solution. The reaction mixture was placed in silicon oil bath 5 and monitored at 900 C. Acetonitrile + 2 eq. TMAOTf Under nitrogen atmosphere, iodonium triflate precursor (0.004g, 6.6 smol) was dissolved in 0.38 ml, dry, degassed d 3 -acetonitrile and combined with 18 pL of 10 TMAF (6.6 pmol) stock solution, with a subsequent addition of tetramethylammonium triflate (0.0015g, 6.6 gmol) to the reaction mixture. The solution was then placed in a silicon oil bath and monitored at 900 C. Benzene no salt 15 Under nitrogen atmosphere, iodonium triflate precursor (0.004g, 6.6 jmol) was dissolved in 0.38 mL dry degassed acetonitrile and combined with 18 pL of TMAF (6.6 pmol) stock solution. The acetonitrile was removed by vacuum and the remaining residue was redissolved in 0.4 ml dry, degassed d 6 -benzene. The solution was passed twice through 0.22 Rm PTFE filter, sealed under nitrogen, and monitored 20 in silicon oil bath at 90* C. Benzene + I eq. TMAOTf Under nitrogen atmosphere, iodonium triflate precursor (0.004g, 6.6 gmol) was dissolved in 0.38 mL dry, degassed acetonitrile and combined with 18 LL of 25 TMAF (6.6 prmol) stock solution. The acetonitrile was removed by vacuum and the remaining residue was redissolved in 0.4 mL dry, degassed d 6 -benzene. The reaction mixture was sealed under nitrogen and monitored in silicon oil bath at 90 *C. Benzene + 2 eq. TMAOTf so Under nitrogen atmosphere, iodonium triflate precursor (.004g, 6.6 tmol) was dissolved in 0.38 mL dry, degassed d 3 -acetonitrile and combined with 18 piL of TIAF (6.6 pmol) stock solution, with a subsequent addition of tetramethylammonium triflate (.0015g, 6.6 gmol) to the reaction mixture. The 48 acetonitrile was removed by vacuum and the remaining residue was redissolved in 0.4 mL d 6 -benzene. The solution was then placed in a silicon oil bath and monitored at 90 OC. 5 The results of these experiments are shown in FIGs. 1 and 2. It is clear that added salt has a large negative impact on the yield of the reaction in acetonitrile, but not as significant an impact on the results for the decomposition reaction performed in the nonpolar solvent benzene. This latter result may be due to the fact that TMAOTf is only sparingly soluble in benzene. 10 Example 29 - Fluorinations ofradiofluorination of MTEB under conventional conditions For each reaction the iodonium precursor Ar-MTEB-OTf (2 mg) was dissolvent in 300 pL of either acetonitrile, DMF, or DMSO. 15 Preparation of Kryptofix 222/K 2

CO

3 1 sF source: A mixture of 50-100 L of

['

8 0]H 2 0 with [ 1 F]fluoride + 15 L of I M K 2 C0 3 (aq) + 800 L CH 3 CN was heated for 3 minutes in a microwave cell at 20 W. The mixture was treated with 800 pL of

CH

3 CN and heated again. Excess solvent was removed under a stream of dry nitrogen at 800 C. 20 Run 1: A solution of Ar-MTEB-OTf (2 mg) in 300 p1L DMF was added to the dried Kryptofix 222/K 2

CO

3 Kl'F source and heated in a microwave (50 W, 1.5 min). No detectable radiolabeled MTEB was seen by radio-TLC. Additional microwave heating for 3 or 6 minutes resulted in no 1 F-MTEB. 25 Run 2: A solution of Ar-MTEB-OTf (2 mg) in 300 pL DMSO was added to the dried Kryptofix 222/K 2

CO

3 KisF source and heated in a conventional oil bath at 1200 C for 15 minutes. No detectable radiolabeled MTEB was seen by radio-TLC. Further heating for 15 or 30 minutes resulted in the formation of no detectable 18

F

30 MTEB. For runs 3 and 4, a solution of [' 8 F]TBAF was prepared by addition of TBAOH to the [ 18 0]H 2 0 solution containing [1'F]fluoride. Drying was performed in 49 vacuo. The resulting solid was treated with 800 pL of CH3CN and dried by heating to 800 C under a stream of dry nitrogen. Run 3: A solution of Ar-MTEB-OTf (2 mg) in 300 pL DMF was added to the 5 [' 8 F]TBAF and heated in at 1500 C oil bath for 15 minutes, 30 minutes, and one hour. No detectable radiolabeled MTEB was seen by radio-TLC. Run 6: A solution of Ar-MTEB-OTf (2 mg) in 300 gL DMSO was added to the [' 8 F]TBAF and heated in at 1200 C oil bath for 15 minutes, 30 minutes, and one 10 hour. A yield of 6.3% of radiolabeled MTEB was seen by radio-TLC. Example 30 - Preparation of 1 8 F-MTEB with salt removal.

[

18 F]TBAF was dried twice with MeCN at 900 C under reduced pressure (-10 mmHg). Ar-MTEB-OTf (2 mg) was dissolved in MeCN (300 pL) and added to the 15 vial containing the dried [' 8 F]TBAF. The reaction mixture was stirred at 900 C and the MeCN was evaporated under reduced pressure (-10 mm Hg). The remaining residue was re-dissolved in 2 mL of dry benzene, passed through 0.22-mm syringe filter, and heated to 1000 C for 20 minutes (radiochemical yield (RCY)= ca 70 %, determined by radio-HPLC and radio-TLC) 20 Example 31 - Preparation of 'F-MTEB with salt removal.

[

18 F]TBAF was dried twice with MeCN at 900 C under reduced pressure (-10 mmHg). Ar-MTEB-OTf (2 mg) was dissolved in MeCN (300 p.L) and added to the vial containing the dried [ 1 8 F]TBAF. The reaction mixture was stirred at 900 C and 25 the MeCN was evaporated under reduced pressure (-10 mm Hg). The remaining residue was re-dissolved in 2 mL of dry benzene, passed through 0.22-mm syringe filter, and heated to 1300 C for 20 minutes (radiochemical yield (RCY)= ca 90 %, determined by radio-HPLC and radio-TLC) 50 Example 32 - Preparation of [sF]-6-Fluoro-L-DOPA. O O St-Bu, O O-t-Bu MeO 01 TfO OMe MeO Ar-LDOPA-OTf 5 Ar-LDOPA-OTf (2 mg) is dissolved in 300 pL of dry acetonitrile and added to a vial containing dry [' 8 F]TBAF. The solution is warmed to 900 C and the solvent is removed under reduced pressure. Dry toluene (500 gL) is added to the residue and the solution is passed through a 0.22 pm PTFE membrane filter and heated (in a sealed vessel) to 130' C for 20 minutes. The solvent is removed under reduced pressure and 10 the residue is treated with 48% HBr (500 gL) and heated at 1400 C for 8 minutes to remove the protecting groups. The [' 8 F]-6-Fluoro-L-DOPA is purified by reverse phase chromatography. Example 33 - General procedure for the preparation offluorinated aryl amino acids i5 and their derivatives. The appropriate (4-methoxyphenyl)aryliodonium triflate (2-3 mg) is dissolved in 300 pL of dry acetonitrile and added to a vial containing dry [' 8 F]TBAF. The solution is warmed to 90' C and the solvent is removed under reduced pressure. Dry toluene or benzene (500 L) is added to the residue and the solution is passed through 20 a 0.22 im PTFE membrane filter and heated (in a sealed vessel) to 130* C for 20 minutes. The solvent is removed under reduced pressure and the residue is treated with 48% HBr (500 pL) and heated at 140 C for 8 minutes to remove the protecting groups. The [1'F]-fluorinated aryl amino acid or derivative is purified by reverse phase chromatography. 25 Example 34 - Preparation of6-Fluoro-L-DOPA. The precursor Ar-LDOPA-OTf (20 mg) was dissolved in 0.7 mL of dry

CD

3 CN and treated with one equivalent of TMAF. The solvent was removed and the 51 residue was dissolved in 0.7 mL of d 6 -benzene, placed in an NMR tube equipped with a PTFE valve, and heated to 140 "C for 20 minutes. 1H and 1 9 F NMR spectra (FIGs. 3 and 4) indicated that the yield of the reaction was 85% and that the yield of 4 fluoroanisole was approximately 1%. 5 Example 35 - Deprotection of 6-Fluoro-L-DOPA. The solvent was removed from the reaction mixture containing crude 6-fluoro L-DOPA (Example 34). The residue was dissolved in 1 mL of 48% aqueous HBr and the solution was heated to 140 *C for 10 minutes. The solution was neutralized with 10 sodium bicarbonate and the water was evaporated. 1 H and 1 9 F NMR spectra (D 2 0) were identical to the authentic standard, as was confirmed by adding independently obtained 6-fluoro-L-DOPA to the NMR tube. A number of embodiments of the invention have been described. 15 Nevertheless, it will be understood that various modifications may be made without departing from the spirit and scope of the invention. Accordingly, other embodiments are within the scope of the following claims. 52

Claims (12)

1. ,A method for making a compound of Formula (3): Ar 2 F 3 wherein: (A) Ar 2 is an aryl or heteroaryl ring system; the method comprising reacting in a polar solvent a compound ME, wherein M is a counter ion, and a compound of Fornula (2): y Ar 1. Ar2 2 wherein: Arl is an electron rich aryl or heteroaryl ring system; Y is a leaving group; and Ar 2 is as defined above; removing the polar solvent from the reaction mixture; corbining the remaining mixture with a nonpolar solvent; filtering the resulting mixture to remove insoluble material; and heating the filtrate; (B) Ar 2 is an aryl or heteroaryl ring system; the method comprising reacting in a nonpolar solvent a compound MF, wherein M is a counter ion, and a impoundd of Formula (2): Ar- Ar 2 wherein: Ar' is an electron ri-h aryl or heteroaryl ring system; Y is a leaving growp; and Ar2 is as defined above; filtering the reaction mixture to remove insoluble material; and heating the filtrate; (C) Ar' is an aryl or beteroaryl ring system; 53 the method comprising reacting in a polar solvent a compound MF, wherein M is a counter ion, and a compound of Formula (2) y Ar 1 - I A: 2 2 wherein: Ar' is an electron rich aryl or heteroaryl ring system; Y is a leaving group; and Ar is as defined above; removing the polar solvent from the reaction mixture; and heating a solution comprising the remaining mixture and a nonpolar solvent.

2. The method of claim 1, wherein Ar '--H is more easily oxidized than benzene, 3, The method of claim I or claim 2, wherein Arl is substituted with at least one substituent having a Hammett o. value of less than zero.

4. The method of claim I or claim 2, wherein Arl is substituted with a substituent chosen from: -(C-Cjo)alkyl, (C-Cjo)haloalkyl. (C 2 -C 1 O)alkenyl. (C m Cwo)alkynyl, -(Cr C)aikyl -C(O)FO-(C C)aikyi, aryl, and heteroaryl, i. The method of any one of the preceding claims, wherein the F is a radioactive isotope of fluorine.

6. The method of any one of the preceding claims, wherein Ar' and Ar 2 are the same. '7. The method of any one of the preceding claims, whereiii Ar' is: R2 RI R ------ I R 4 R 5 wherein: 54 R, R2, R 3 , Re, and R 5 arc independently chosen from: H -(C-C 10 )alky ~(C Cio)haloalkyl, (C-CaiO)alkenyl, (C-C 1 )alkynyl O-(C 1 Co)alky -C(O)O-(Cl C 1 )alkiy aryl, and heteroaryt, or two or more of R'. R2, Rt R4. and RW come together to form a ifused aryl or heteroaryl ring system, 8 The method of any one of the preceding claims, wherein Ar is chosen from a phenylalanine derivative. tyTosine derivative, tryptophan derivative, histidine derivative, and an estradiol derivative.

9. The method of any one of the preceding claims, wherein Ar' is chosen from: OMe CN MeO oMe Me OMe OMe CF, 55 N N N 'S -Pc 5 oK0 2 10 O OO 0 010 oop 4 O)p4 OP4 O)p4 P2N P 2 P. P2 p NN N N NC pPs 1 N O P56 4 1 X 0 0 NNN P6j P 3 56 P% N'P 2 PI p PI .P 2 N N OPs Ops 6P Kit 'P 'tN' 2p N P OP PI ,&? rQ ~p 2 I 22 N N N r~s N N P 6 ps N 57 P 2 P P 1 N Nm pl NPp PN p pl 1' p2 N N POP P 8 N N 'PN K t tp 2 K , 2 N.p N N N p 7 -O 2 N7 op3-CN NO7" P pp2 0 PI 2 N' N O pC-P3OO' - N -~ /~NOP 3 N ON OP N S58 wherein: each ofP 1 P 2 and P are independently a nitrogen protecting group, or Pt and P 2 come together to form a single nitrogen protecting group; each of P3 P 4 and P 7 are independently an alcohol protecting group, or P 3 and P 4 come together to form a single oxygen protecting group; and P 5 is a carboxyli acid protecting group.

10. The method of any one of the preceding claims wherein in method (A) or (B) as defined in claim 1: the insoluble material comprises insoluble saints; or the solvent is removed from the filtrate prior to heating.

11. The method of any one of claims 1 to 9 wherein in method (C) as defined in claim 1, the mixture comprising the nonpolar solvent is filtered prior to heating.

12. The method of any one of claims I to 9 wherein in method (A) or (C) as defined in claim 1, the polar solvent is chosen from acetonitrile, acetone, dichloromethane, ethyl acetate, tetrahydrofuran, dimethyiformamide, 1 2-difluorobenzene, beuzotrifluoride and mixtures thereof 11 The method of any one of the preceding claims, wherein the compound of Formula (2) of method (A, (B)or (C) is chosen front: 59 P N N N ,AI 7zl N 0 0 ~ 0N Ar AN op 4 S5p4 yI '1 Y OP 4 P N N 'I N 0 -O ArYrr Wherein: each ofPand P are independently a nitrogen protecting group, or Pl and P come together to fonn a single nitrogen protecting group; ech ofP and P 4 are independently an alcohol protecting group, or P 3 and P 4 come together to folrm a single oxygen protecting group; and P 5 is a carboxylic acid protecting group; <2 N Ar ' Ar1'ArA CNNCN CN S S N 'Ar- Ar Ar ON ON CN and 60 op 3 P 4 -o Y Ar' wherein: each of P and 4 are independently an alcohol protecting group. 14, The method of claim 13 wherein the compound of Formula (2) of method (A), (B) or (C) as defined in claim I is chosen from: 0 0 0 0 t-BuN O-K A -Bu t-Bu, 0 A N A-Bu Meo y, Q f'o0, NO Ar0 Me OTfO 0Me or MeO

15. The method of any one of the preceding claims, where in the compound of Formula (3) is chosen from: pN p 2 2 K Y 2 'N" N N F0 F - 0 oP F op 4 OP op p p 2 P P2 N 0 F rF 'N 0 0N NOR 3 'OP 3 OP p F wherein: 61 each of Pand P 2 are independently a nitrogen protecting group, or P and P 2 conme together to form a single nitrogen protecting group; each of p 3 , and P 4 are independently an alcobol protecting group, or P and P come together to tbrn a single oxygen protecting group; and P 5 is a carboxylic acid protecting group; N F N N NF N CN CN ON and OPPm Pp Op F 16i The method of claim i 5 wherein, the compound of Formula (3) is chosen ftom: 0 0 t<Bu A N OtIBU N 0 N'~ 0 j OH F F OMe H ON'lle or H

16. The method ofany one ofthe preceding claims, wheein the nonpolar solvent is chosen fro-m; benze,.,ne, toluene, o-xylenea, n-xyleniesp-xylene, ethyli benz7ene, carbonrachoie he-xane, cyoIclhexan1e, fluorobenzene;- chlorobenzene ntoenee andnxursteef 62 M8. The method of any one of the preceding claims, wherein Y is chosen from triflate, nesylate, nonaflate, hexaflate, tosylate, nosylate, brosylate, perfluoroilkyl sutbnatc, tetraphenylborate, hexafluorophosphate, trifluoroacetate, tetrafluoroborate, perchlorate, perfluoroalkylcarboxylate, chloride, bromide, and iodide. 1-9. The method of any one of the preceding claims, wherein M is chosen from: potassium. sodium, cesium, complexes of lithium, sodium, potassium, or cesium with cryptands or crown ethers, tetrasubstituted armonium cations, and phosphonium cautions.

20. Product obtained by the method of any one of the preceding chims.

21. A method for making a compound of Formula (3) as defined in claim I or product as defined in claim 20 substantially as hereinbefore described with reference to the accompanying description, drawings and examples, excluding comparative examples, if any, 63