AU2012216752B2 - Quinazoline based EGFR inhibitors containing a zinc binding moiety - Google Patents

Abstract

The present invention relates to quinazoline containing zinc-binding moiety based derivatives that have enhanced and unexpected properties as inhibitors of epidermal growth factor receptor tyrosine kinase (EGFR-TK) and their use in the treatment of EGFR-TK related diseases and disorders such as cancer. The said derivatives may further act as HDAC inhibitors.

Description

AUSTRALIA Regulation 3.2 Patents Act 1990 Complete Specification Standard Patent APPLICANT: Curis, Inc. Invention Title: QUINAZOLINE BASED EGFR INHIBITORS CONTAINING A ZINC BINDING MOIETY The following statement is a full description of this invention, including the best method of performing it known to me: WO 20081033749 PCT/US2007/077977 QUINAZOLINE BASED EGFR INHIBITORS CONTAINING A ZINC BINDING MOIETY RELATED APPLICATIONS SECTION This application claims the benefit of U.S. Provisional Application No. 60/843,644, 5 filed on September 11, 2006 and U.S. Provisional Application No. 60/895,873, filed on March 20, 2007, the contents of which are hereby incorporated by reference. BACKGROUND OF THE INVENTION The epidermal growth factor receptor (EGFR, Erb-B1) belongs to a family of proteins, involved in the proliferation of normal and malignant cells (Artega, C.L., J Clin 10 Oncol 19, 2001, 32-40). Overexpression of Epidermal Growth Factor Receptor (EGFR) is present in at least 70% of human cancers (Seymour, L.K., Curr Drug Targets 2, 2001, 117 133) such as, non-small cell lung carcinomas (NSCLC), breast cancers, gliomas, squamous cell carcinoma of the head and neck, and prostate cancer (Raymond et al., Drugs 60 Suppl 1, 2000, discussion 41-2; Salomon et al., Crit Rev Oncol Henatol 19, 1995, 183-232; 15 Voldborg et al., Ann Oncol 8, 1997, 1197-1206). The EGFR-TK is therefore widely recognized as an attractive target for the design and development of compounds that can specifically bind and inhibit the tyrosine kinase activity and its signal transduction pathway in cancer cells, and thus can serve as either diagnostic or therapeutic agents. For example, the EGFR tyrosine kinase (EGFR-TK) reversible inhibitor, TARCEVA@, was recently 20 approved by the FDA for treatment of NSCLC and advanced pancreatic cancer. Other anti EGFR targeted molecules have also been approved such as IRESSA@. 0 HN 0^'o tessa (z1 839) Tacva@ Despite the early success of Tarceva, it has become clear that selectively targeting individual kinases can lead to the development of drug resistant tumors. Cells that have 25 developed mutations within the drug/kinase binding pocket display a growth advantage in the presence of drug eventually leading to disease progression. Current clinical strategies 1 WO 2008/033749 PCT/US2007/077977 aimed at combining these molecularly targeted drugs with standard chemotherapeutics, radiation, or other targeted agents will lead to novel strategies to improve overall response rate and increase the number of complete remissions. Furthermore, elucidation of the complex and multifactorial nature of various 5 diseases that involve multiple pathogenic pathways and numerous molecular components suggests that multi-targeted therapies may be advantageous over mono-therapies. Recent combination therapies with two or more agents for many such diseases in the areas of oncology, infectious disease, cardiovascular disease and other complex pathologies demonstrate that this combinatorial approach may provide advantages with respect to 10 overcoming drug resistance, reduced toxicity and, in some circumstances, a synergistic therapeutic effect compared to the individual components. Certain cancers have been effectively treated with such a combinatorial approach; however, treatment regimes using a cocktail of cytotoxic drugs often are limited by dose limiting toxicities and drug-drug interactions. More recent advances with molecularly 15 targeted drugs have provided new approaches to combination treatment for cancer, allowing multiple targeted agents to be used simultaneously, or combining these new therapies with standard chemotherapeutics or radiation to improve outcome without reaching dose limiting toxicities. However, the ability to use such combinations currently is limited to drugs that show compatible pharmacologic and pharmacodynamic properties. In addition, the 20 regulatory requirements to demonstrate safety and efficacy of combination therapies can be more costly and lengthy than corresponding single agent trials. Once approved, combination strategies may also be associated with increased costs to patients, as well as decreased patient compliance owing to the more intricate dosing paradigms required. In the field of protein and polypeptide-based therapeutics it has become 25 commonplace to prepare conjugates or fusion proteins that contain most or all of the amino acid sequences of two different proteins/polypeptides and that retain the individual binding activities of the separate proteins/polypeptides. This approach is made possible by independent folding of the component protein domains and the large size of the conjugates that permits the components to bind their cellular targets in an essentially independent 30 manner. Such an approach is not, however, generally feasible in the case of small molecule therapeutics, where even minor structural modifications can lead to major changes in target binding and/or the pharmacokinetic/pharmacodynamic properties of the resulting molecule. 2 WO 20081033749 PCT/US2007/077977 The use of EGFR inhibitors in combination with histone deacetylases (HDAC) has been shown to produce synergistic effects. Histone acetylation is a reversible modification, with deacetylation being catalyzed by a family of enzymes termed HDAC's. HDAC's are represented by X genes in humans and are divided into four distinct classes (JMol Biol, 5 2004, 338:1, 17-31). In mammalians class I HDAC's (HDAC1-3, and HDAC8) are related to yeast RPD3 HDAC, class 2 (HDAC4-7, HDAC9 and HDAC10) related to yeast HDA1, class 4 (HDAC 11), and class 3 (a distinct class encompassing the sirtuins which are related to yeast Sir2). Csordas, Biochem. J., 1990, 286: 23-38 teaches that histones are subject to post 10 translational acetylation of the, c-amino groups of N-terminal lysine residues, a reaction that is catalyzed by histone acetyl transferase (HAT 1). Acetylation neutralizes the positive charge of the lysine side chain, and is thought to impact chromatin structure. Indeed, access of transcription factors to chromatin templates is enhanced by histone hyperacetylation, and enrichment in underacetylated histone H4 has been found in transcriptionally silent regions 15 of the genome (Taunton et al., Science, 1996, 272:408-411). In the case of tumor suppressor genes, transcriptional silencing due to histone modification can lead to oncogenic transformation and cancer. Several classes of HDAC inhibitors currently are being evaluated by clinical investigators. The first FDA approved HDAC inhibitor is Suberoylanilide hydroxamic acid 20 (SAHA, Zolinza@) for the treatment of cutaneous T-cell lymphoma (CTCL). Other HDAC inhibitors include hydroxamic acid derivatives; PXD101 and LAQ824, are currently in the clinical development. In the benzamide class of HDAC inhibitors, MS-275, MGCDO 103 and CI-994 have reached clinical trials. Mourne et al. (Abstract #4725, AACR 2005), demonstrate that thiophenyl modification of benzamides significantly enhance HDAC 25 inhibitory activity against HDAC 1. Recent advances suggest that EGFR-TK inhibitors in combination with HDAC inhibitors may provide advantageous results in the treatment of cancer. For example, co treatment with SAHA significantly increased EGFR2 antibody trastuzumab-induced apoptosis of BT-474 and SKBR-3 cells and induced synergistic cytotoxic effects against the 30 breast cancer cells (Bali, Clin. Cancer Res., 2005, 11, 3392). HDAC inhibitors, such as SAHA, have demonstrated synergistic antiproliferative and apoptotic effects when used in combination with gefitinib in head and neck cancer cell lines, including lines that are resistant to gefitinib monotherapy (Bruzzese et al., Proc. AACR, 2004). Pretreating 3 WO 2008/033749 PCT/US2007/077977 gefitinib resistant cell lines with the HDAC inhibitor, MS-275, led to a growth-inhibitory and apoptotic effect of gefitinib similar to that seen in gefitinib-sensitive NSCLC cell lines including those harboring EGFR mutations (Witta S.E., et al., Cancer Res 66:2, 2006, 944 50). The HDAC inhibitor PXD101 has been shown to act synergistically to inhibit 5 proliferation with the EGFR1 inhibitor Tarceva@ (erlotinib) (W02006082428A2). Current therapeutic regimens of the types described above attempt to address the problem of drug resistance by the administration of multiple agents. However, the combined toxicity of multiple agents due to off-target side effects as well as drug-drug interactions often limits the effectiveness of this approach. Moreover, it often is difficult to combine 10 compounds having differing pharmacokinetics into a single dosage form, and the consequent requirement of taking multiple medications at different time intervals leads to problems with patient compliance that can undermine the efficacy of the drug combinations. In addition, the health care costs of combination therapies may be greater than the cost of single molecule therapies. Furthermore, it may be more difficult to obtain regulatory 15 approval of a combination therapy since the burden for demonstrating activity/safety of a combination of two agents may be greater than for a single agent (Dancey J & Chen H, Nat. Rev. Drug Dis., 2006, 5:649). The development of novel agents that target multiple therapeutic targets selected not by virtue of cross reactivity, but through rational design will help improve patient outcome while avoiding these limitations. Thus, enormous efforts are 20 still directed to the development of selective anti-cancer drugs as well as to new and more efficacious combinations of known anti-cancer drugs. SUMMARY OF THE INVENTION The present invention relates to quinazolines containing zinc-binding moiety based derivatives that have enhanced and unexpected properties as inhibitors of epidermal growth 25 factor receptor tyrosine kinase (EGFR-TK). The compounds of the present invention also act as HDAC or matrix metalloproteinase (MMP) inhibitors and HER2 inhibitors. Surprisingly, these compounds are active at multiple therapeutic targets and are effective for treating diseases related to EGFR-TK activity, HDAC activity and/or HER2 activity, such as cancer and proliferative diseases. Moreover, it has even more surprisingly been found 30 that the compounds have enhanced activity when compared to the activities of separate molecules individually having the EGFR-TK and HDAC activities and combinations thereof. In other words, the combination of pharmacophores into a single molecule may 4 WO 2008/033749 PCT/US2007/077977 provide a synergistic effect as compared to the individual pharmacophores. More specifically, it has been found that it is possible to prepare compounds that simultaneously contain a first portion of the molecule that binds zinc ions and thus permits inhibition of HDAC and/or matrix metalloproteinase (MMP) activity and at least a second portion of the 5 molecule that permits binding to a separate and distinct target that inhibits EGFR-TK and thus provide therapeutic benefit. Preferably, the compounds of the present invention inhibit EGFR-TK, HER2 and HDAC activity. Accordingly, the present invention provides a compound having the general Formula I: X 'Ar C--B N 10 R4 or its geometric isomers, enantiomers, diastereomers, racemates, pharmaceutically acceptable salts, prodrugs and solvates thereof, wherein Ar is aryl, substituted aryl heteroaryl or substituted heteroaryl; Q is absent or substituted or unsubstituted alkyl; 15 X is 0, S, NH, or alkylamino; B is a direct bond or straight or branched, substituted or unsubstituted alkyl, substituted or unsubstituted alkenyl, substituted or unsubstituted alkynyl, arylalkyl, arylalkenyl, arylalkynyl, heteroarylalkyl, heteroarylalkenyl, hetoroarylalkynyl, heterocyclylalkyl, heterocyclylalkenyl, heterocyclylalkynyl, 20 aryl, heteroaryl, heterocyclyl, cycloalkyl, cycloalkenyl, alkylarylalkyl, alkylarylalkenyl, alkylarylalkynyl, alkenylarylalkyl, alkenylarylalkenyl, alkenylarylalkynyl, alkynylarylalkyl, alkynylarylalkenyl, alkynylarylalkynyl, alkylheteroarylalkyl, alkylheteroarylalkenyl, alkylheteroarylalkynyl, alkenylheteroarylalkyl, alkenyiheteroarylalkenyl, alkenylheteroarylalkynyl, 25 alkynylheteroarylalkyl, alkynylheteroarylalkenyl, alkynylheteroarylalkynyl, alkylheterocyclylalkyl, alkylheterocyclylalkenyl, alkylhererocyclylalkynyl, alkenylheterocyclylalkyl, alkenylheterocyclylalkenyl, alkenylheterocyclylalkynyl, alkynylheterocyclylalkyl, alkynylheterocyclylalkenyl, alkynylheterocyclylalkynyl, alkylaryl, alkenylaryl, 5 WO 2008/033749 PCT/US2007/077977 alkynylaryl, alkylheteroaryl, alkenylheteroaryl, or alkynylhereroaryl, which one or more methylenes can be interrupted or terminated by 0, S, S(O), SO 2 , N(Rs), C(O), substituted or unsubstituted aryl, substituted or unsubstituted heteroaryl, substituted or unsubstituted heterocyclic; where R 8 is hydrogen, 5 acyl, aliphatic or substituted aliphatic; In one embodiment, the linker B is between 1-24 atoms, preferably 4-24 atoms, preferably 4-18 atoms, more preferably 4-12 atoms, and most preferably about 4-10 atoms. C is selected from: W I I 10 (a) R R 7 ; where W is O or S; Y is absent, N, or CH; Z is N or CH; R7 and

R

9 are independently hydrogen, OR' or aliphatic group, wherein R' is hydrogen, aliphatic, substituted aliphatic or acyl; provided that if R 7 and R 9 are both present, one of R 7 or R must be OR' and if Y is absent, R9 must be OR'; and Rs is hydrogen, acyl, aliphatic or substituted aliphatic; 15 W HO R1o (b) J ; where W is O or S; J is O NH, or NCH 3 ; and Rio is hydrogen or lower alkyl; W HOZ1 Y (c) ;) where W is 0 or S; Yi and Zi are independently N, C or CH; and 20

R

1 NH 2

R

2 -Z Y

R

3 | | (d) R 12 Ril ;where Z, Y, and W are as previously defined; Ru and R12 are independently selected from hydrogen or aliphatic; R1, R 2 and R 3 are independently selected from hydrogen, hydroxy, amino, halogen, alkoxy, substituted alkoxy, alkylamino, substituted alkylamino, dialkylamino, 25 substituted dialkylamino, substituted or unsubstituted alkylthio, substituted or 6 WO 2008/033749 PCT/US2007/077977 unsubstituted alkylsulfonyl, CF 3 , CN, N 3 , NO 2 , sulfonyl, acyl, aliphatic, substituted aliphatic, aryl, substituted aryl, heteroaryl, substituted heteroaryl, heterocyclic, and substituted heterocyclic; R4 is independently selected from hydrogen, hydroxy, amino, halogen, CF 3 , CN, 5 N 3 , NO 2 , sulfonyl, acyl, substituted or unsubstituted alkyl, substituted or unsubstituted alkenyl, substituted or unsubstituted alkynyl, arylalkyl, arylalkenyl, arylalkynyl, heteroarylalkyl, heteroarylalkenyl, heteroarylalkynyl, heterocyclylalkyl, heterocyclylalkenyl, heterocyclylalkynyl, aryl, heteroaryl, heterocyclyl, cycloalkyl, cycloalkenyl, alkylarylalkyl, alkylarylalkenyl, 10 alkylarylalkynyl, alkenylarylalkyl, alkenylarylalkenyl, alkenylarylalkynyl, alkynylarylalkyl, alkynylarylalkenyl, alkynylarylalkynyl, alkyiheteroarylalkyl, alkylheteroarylalkenyl, alkylheteroarylalkynyl, alkenylheteroarylalkyl, alkenylheteroarylalkenyl, alkenylheteroarylalkynyl, alkynylheteroarylalkyl, alkynylheteroarylalkenyl, alkynyiheteroarylalkynyl, alkylheterocyclylalkyl, 15 alkylheterocyclylalkenyl, alkythererocyclylaikynyl, alkenylheterocyclylalkyl, alkenylheterocyclylalkenyl, alkenylheterocyclylalkynyl, alkynylheterocyclylalkyl, alkynylheterocyclylalkenyl, or alkynylheterocyclylalkynyl, which one or more methylenes can be interrupted or terminated by 0, S, S(0), SO 2 , N(Rs), C(O), substituted or unsubstituted 20 aryl, substituted or unsubstituted heteroaryl, substituted or unsubstituted heterocyclic; where Rs hydrogen, acyl, aliphatic or substituted aliphatic. BRIEF DESCRIPTION OF THE DRAWINGS The foregoing and other objects, features and advantages of the invention will be apparent from the following more particular description of preferred embodiments of the 25 invention, as illustrated in the accompanying drawings in which like reference characters refer to the same parts throughout the different views. The drawings are not necessarily to scale, emphasis instead being placed upon illustrating the principles of the invention. Figure 1 (a) depicts a graph of EGFR enzyme assay results, (b) depicts a graph of HDAC enzyme assay results. 30 Figure 2 illustrates inhibition of HDAC and EGFR in MDA-MB-468 breast cancer cell line: (a) Ac-H4 Accumulation, (b) Ac-H3 Accumulation, (c) EGFR inhibition. 7 WO 2008/033749 PCT/US2007/077977 Figure 3 shows comparative data of anti-proliferative activity against several different cancer cell lines: (a) pancreatic cancer (BxPC3), (b) NSCLC (H1703), (c) breast cancer (MDA-MB-468), (d) prostate cancer (PC3). Figure 4 illustrates the potency of compound 12 induction of apoptosis in cancer 5 cells: (a) HCT-1 16 (colon, 24 hours), (b) SKBr3 (breast, 24 hours). Figure 5 shows the efficacy of compound 12 in A431 Epidermoid Tumor Xenograft Model (IP Dosing). Figure 6 shows the efficacy of compound 12 in H358 NSCLC Xenograft Model (2 Min IV infusion). 10 Figure 7 shows the efficacy of compound 12 in H292 NSCLC Xenograft Model (2 Min IV infusion). Figure 8 shows the efficacy of compound 12 in BxPC3 Pancreatic Cancer Xenograft Model (2-Mm IV infusion). Figure 9 shows the efficacy of compound 12 in PC3 Prostate Cancer Xenograft 15 Model (2-Min IV infusion). Figure 10 shows the efficacy of compound 12 in HCT 116 Colon Cancer Xenograft Model (2-Min IV infusion). Figure l IA shows the percent of change in tumor size in animals treated with compound 12 or vehicle in A549 NSCLC Xenograft model. 20 Figure 1 B shows the percent of change in tumor size in animals treated with Erlotinib and control in A549 NSCLC Xenograft model. Figure 12A shows the percent of change in tumor size in animals treated with compound 12, Erlotinib or vehicle in HPAC pancreatic cancer cells. Figure 12B shows the percent of change in body weight in animals treated with 25 compound 12, Erlotinib or vehicle in HPAC pancreatic cancer cells. Figure 13 shows the concentration of compound 12 in plasma, lung and colon after administration of hydrochloride, citrate, sodium and tartrate salts of compound 12. Figure 14 shows the concentration of compound 12 in the plasma of mice administered compound 12 in 30% CAPTISOL. 30 Figure 15 shows the percent change in mouse body weight after administration of an IV dose of compound 12 (25, 50, 100, 200 and 400 mg/kg) in 30% CAPTISOL. Figure 16 shows the percent change in mouse body weight after 7 days repeat IP dosing of compound 12 (25, 50, 100, 200 and 400 mg/kg) in 30% CAPTISOL. 8 WO 2008/033749 PCT/US2007/077977 Figure 17 shows the percent change in rat body weight after administration of an IV dose of compound 12 (25, 50, 100 and 200 mg/kg) in 30% CAPTISOL. DETAILED DESCRIPTION OF THE INVENTION In a first embodiment of the compounds of the present invention are compounds 5 represented by formula (I) as illustrated above, or its geometric isomers, enantiomers, diastereomers, racemates, pharmaceutically acceptable salts, prodrugs and solvates thereof. In a second embodiment of the compounds of the present invention are compounds represented by formula (11) as illustrated below, or its geometric isomers, enantiomers, diastereomers, racemates, pharmaceutically acceptable salts, prodrugs and solvates thereof: X Ar W R9 BN 10 R 8

R

7

R

4 (II) wherein Q, X, B, Y, W, Z, Ar, R 4 , R7, Rs, and R 9 are as previously defined. In a third embodiment of the compounds of the present invention are compounds represented by formula (III) as illustrated below, or its geometric isomers, enantiomers, diastereomers, racemates, pharmaceutically acceptable salts, prodrugs and solvates thereof: X Ar 0 R' N R.~N 15 R8 R7 R 11 wherein Q, X, B, Y, Ar, R', R4, R 7 , and R 8 are as previously defined. In a fourth embodiment of the compounds of the present invention are compounds represented by formula (IV) as illustrated below, or its geometric isomers, enantiomers, diastereomers, racemates, pharmaceutically acceptable salts, prodrugs and solvates thereof: HN "AR20 ON NOs YB432-1 R22 20 R 8

R

7 R4(IV) wherein B 1 is absent, 0, S, aryl, heteroaryl, heterocylic, NH or alkylamino; B 2 is absent, CI-C6 alkyl, C2-C6 alkenyl, C2-C6 alkynyl, aryl, heteroaryl, heterocyclic, CO, SO, or SO 2 ; 9 WO 2008/033749 PCT/US2007/077977

B

3 is absent, 0, NH, alkylamino, C 1

-C

6 alkyl, C2-C6 alkenyl, C2-C6 alkynyl, aryl, heteroaryl, or heterocyclic; B 4 is absent, C-C 8 alkyl, C2-C8 alkenyl, C2-C8 alkynyl, heterocyclic, heteroaryl or aryl; R 20 , R 2 1 , R 2 2 are independently selected from R 1 ; Q, Y, R',

R

4 , R 7 , and R 8 are as previously defined. 5 In a fifth embodiment of the compounds of the present invention are compounds represented by formula (V) as illustrated below, or its geometric isomers, enantiomers, diastereomers, racemates, pharmaceutically acceptable salts, prodrugs and solvates thereof: SHNR20 R',O N j Y B4- B3 B2- B 1 N -i" R21 II Q R 2 2 Ra

R

7 / N'

M

5

-M

4

-M

3

-M

2

-M

1 (V) wherein B 1 is absent, 0, S, aryl, heteroaryl, heterocylic, NH or alkylamino; B 2 is absent, C 10 C 6 alkyl, C 2

-C

6 alkenyl, C 2

-C

6 alkynyl, aryl, heteroaryl, heterocyclic, CO, SO, or SO 2 ; B 3 is absent, 0, NH, alkylamino, C 1

-C

6 alkyl , C 2

-C

6 alkenyl, C 2

-C

6 alkynyl, aryl, heteroaryl, or heterocyclic; B 4 is absent, C 1

-C

8 alkyl , C 2

-C

8 alkenyl, C 2

-C

8 alkynyl, heterocyclic, heteroaryl or aryl; Mi is absent, C-C 6 alkyl, 0, S, SO, SO 2 , NH, alkylamine, CO, aryl, heteroaryl; M 2 is absent, C 1

-C

6 alkyl, C 2

-C

6 alkenyl, or C 2

-C

6 alkynyl; M 3 is absent, CI-C 6 15 ailcyl, 0, S, SO, S02, NH, alkylamine, aryl, heteroaryl; M 4 is absent, CI-C 6 alkyl, C 2

-C

6 alkenyl, or C 2

-C

6 alkynyl; M 5 is OH, SH, NR 7

R

8 , C0 2

R

8 , SOR 8 , SO 2 Rs, C 1

-C

6 alkyl, C 2

-C

6 alkenyl, C 2

-C

6 alkynyl, aryl, heteroaryl, or heterocyclic; R 20 , R 21 , R 22 are independently selected from R 1 ; Q, Y, R', R 7 , and Rs are as previously defined. In a sixth embodiment of the compounds of the present invention are compounds 20 represented by formula (VI) as illustrated below, or its geometric isomers, enantiomers, diastereomers, racemates, pharmaceutically acceptable salts, prodrugs and solvates thereof:

R

20 HN R21

M

5

-M

4

-M

3

-M

2

-M

1 N R 2 1 0 R22 R'O N kYB4-B3-B2-B1 N I I

R

8

R

7 (VI) wherein B 1 is absent, 0, S, aryl, heteroaryl, heterocylic, NH or alkylamino; B 2 is absent, C

C

6 alkyl, C 2

-C

6 alkenyl, C 2

-C

6 alkynyl, aryl, heteroaryl, heterocyclic, CO, SO, or SO 2 ; B 3 is 25 absent, 0, NH, alkylamino, C 1

-C

6 alkyl , C 2

-C

6 alkenyl, C 2

-C

6 alkynyl, aryl, heteroaryl, or 10 WO 2008/033749 PCT/US2007/077977 heterocyclic; B 4 is absent, C 1 -Cs alkyl , C 2

-C

8 alkenyl, C 2

-C

8 alkynyl, heterocyclic, heteroaryl or aryl; M 1 is absent, C 1

-C

6 alkyl, 0, S, SO, SO 2 , NH, alkylamine, CO, aryl, heteroaryl; M 2 is absent, C 1

-C

6 alkyl, C 2

-C

6 alkenyl, or C 2

-C

6 alkynyl; M 3 is absent, CI-C 6 alkyl, 0, S, SO, S02, NH, alkylamine, aryl, heteroaryl; M 4 is absent, C 1

-C

6 alkyl, C 2

-C

6 5 alkenyl, or C 2

-C

6 alkynyl; M 5 is OH, SH, NR 7

R

8 , C0 2

R

8 , SORs, S0 2 Rs, C 1

-C

6 alkyl, C 2

-C

6 alkenyl, C 2

-C

6 alkynyl, aryl, heteroaryl, or heterocyclic; R 20 , R 21 , R 2 2 are independently selected from R 1 ; Q, Y, R', R 7 , and Rs are as previously defined. In each of the above, B 1 can be absent or is an oxygen, B 2 is absent or is an alkyl, alkenyl, alkynyl, aryl or heteroaryl (e.g., furyl, such as 2,5-furyl), B 3 is absent, a heteroaryl 10 (e.g., furyl), and/or B 4 is an alkyl, alkenyl or alkynyl wherein in each case, the alkyl, alkenyt or alkynyl can be interrupted with or terminated by an 0, S, NH or alkylamino. In a seventh embodiment of the compounds of the present invention are compounds represented by formula (VII) as illustrated below, or its geometric isomers, enantiomers, diastereomers, racemates, pharmaceutically acceptable salts, prodrugs and solvates thereof: X Ar C -B -zN

N-

15 R4 (VII) or its geometric isomers, enantiomers, diastereomers, racemates, pharmaceutically acceptable salts, prodrugs and solvates thereof, wherein Ar is aryl, substituted aryl heteroaryl or substituted heteroaryl; Q is absent or substituted or unsubstituted alkyl; 20 X is 0, S, NH, or alkylamino; B is a direct bond or straight or branched, substituted or unsubstituted alkyl, substituted or unsubstituted alkenyl, substituted or unsubstituted alkynyl, arylalkyl, arylalkenyl, arylalkynyl, heteroarylalkyl, heteroarylalkenyl, heteroarylalkynyl, heterocyclylalkyl, heterocyclylalkenyl. heterocyclylalkynyl, 25 aryl, heteroaryl, heterocyclyl, alkylarylalkyl, alkylarylalkenyl, alkylarylalkynyl, alkenylarylalkyl, alkenylarylalkenyl, alkenylarylalkynyl, alkynylarylalkyl, alkynylarylalkenyl, alkynylarylalkynyl, alkylheteroarylalkyl, alkyiheteroarylalkenyl, alkylheteroarylalkynyl, alkenylheteroarylalkyl, alkenylheteroarylalkenyt, alkenylheteroarylalkynyl, alkynyiheteroarylalkyl, 11 WO 2008/033749 PCT/US2007/077977 alkynylheteroarylalkenyl, alkynylheteroarylalkynyl, alkylheterocyclylalkyl, alkyiheterocyclylalkenyl, alkylhererocyclylalkynyl, alkenylheterocyclylalkyl, alkenylheterocyclylalkenyl, alkenylheterocyclylalkynyl, alkynylheterocyclylalkyl, alkynytheterocyclylalkenyl, or alkynylheterocyclylalkynyl, which one or more 5 methylenes can be interrupted or terminated by 0, S, S(O), SO 2 , N(R 8 ), C(O), substituted or unsubstituted aryl, substituted or unsubstituted heteroaryl, substituted or unsubstituted heterocyclic; where RS is hydrogen or aliphatic group; C is selected from: W RsZ Y~ I I (a) R 8

R

7 ; where W is O or S; Y is absent, N, or CH; Z is N or CH; R 7 and 10 R 9 are independently hydrogen, hydroxy, aliphatic group, provided that if R 7 and R 9 are both present, one of R 7 or R 9 must be hydroxy and if Y is absent, R 9 must be hydroxy; and R 8 is hydrogen or aliphatic group; W HO R10 (b) ; where W is 0 or S; J is 0 NH, or NCH 3 ; and Rio is hydrogen or 15 lower alkyl; W HO Z1 Y (c) ; where W is 0 or S; Yi and Z, are independently N, C or CH; and

R

1

NH

2 R2 ZY

R

3 I I (d) R 1 2

R

1 ; where Z, Y, and W are as previously defined; R1 and 20 R 12 are independently selected from hydrogen or aliphatic; R 1 , R 2 and R 3 are independently selected from hydrogen, hydroxy, amino, halogen, alkoxy, alkylamino, dialkylamino, CF 3 , CN, NO 2 , sulfonyl, acyl, aliphatic, substituted aliphatic, aryl, substituted aryl, heteroaryl, substituted heteroaryl, heterocyclic, and substituted heterocyclic; 12 WO 2008/033749 PCT/US2007/077977

R

4 is independently selected from hydrogen, hydroxy, amino, halogen, substituted or unsubstituted alkoxy, (e.g., alkoxyalkoxy), substituted or unsubstituted alkylamino, substituted or unsubstituted dialkylamino, CF 3 , CN, NO 2 , sulfonyl, acyl, aliphatic, and substituted aliphatic. 5 In a eighth embodiment of the compounds of the present invention are compounds represented by formula (VIII) as illustrated below, or its geometric isomers, enantiomers, diastereomers, racemates, pharmaceutically acceptable salts, prodrugs and solvates thereof: R20 H1 HN R21 I I 2 7 8

R

7 4 C N (VIII) wherein Ra and Rb are hydrogen or taken together with the carbon atom they attached to 10 form a carbonyl; n is 0-9; R 2 0 , R 21 , R 22 are independently selected from Ri; Xi is 0, S or NH; Q, Y, R 4 , R 7 , and Rs are as previously defined. In a ninth embodiment of the compounds of the present invention are compounds represented by formula (IX) as illustrated below, or its geometric isomers, enantiomers, diastereomers, racemates, pharmaceutically acceptable salts, prodrugs and solvates thereof: O HN R 2 1 R N X1R b R2 1

R

8 OH l& 15 R4N (IX) wherein Ra and Rb are hydrogen or taken together with the carbon atom they attached to form a carbonyl; n is 0-9; R 20 , R 21 , R 22 are independently selected from Ri; Xi is 0, S or NH; Q, Y, R 4 , Rs, and R 9 are as previously defined. In a tenth embodiment of the compounds of the present invention are compounds 20 represented by formula (X) as illustrated below, or its geometric isomers, enantiomers, diastereomers, racemates, pharmaceutically acceptable salts, prodrugs and solvates thereof:

R

8 HN " 720 R R b R R22 R 3 4 NX) 13 WO 2008/033749 PCT/US2007/077977 wherein Ra and Rb are hydrogen or taken together with the carbon atom they attached to form a carbonyl; Rc is absent or selected from alkyl, alkenyl, and alkynyl; n is 0-7; Xi is 0, S or NH; G is Ari, Ari-X 2 or Ari-alkyl-X 2 , where Ari is independently selected Ar and X 2 is 0, S or NH; R 2 0 , R21, R 2 2 are independently selected from Ri; Q, R 4 , Rs, and R9 are as 5 previously defined. In a eleventh embodiment of the compounds of the present invention are compounds represented by formula (XI) as illustrated below, or its geometric isomers, enantiomers, diastereomers, racemates, pharmaceutically acceptable salts, prodrugs and solvates thereof:

NH

2 x Ar X,

R

12

R

11 Ra N (XI) 10 wherein P, and Rb are hydrogen or taken together with the carbon atom they attached to form a carbonyl; n is 0-9; X 1 is 0, S or NH; Q, X, Y, Ar, R 1 , R2, R 3 , R 4 , R 1 , and R 12 are as previously defined. In a twelfth embodiment of the compounds of the present invention are compounds represented by formula (XII) as illustrated below, or its geometric isomers, enantiomers, 15 diastereomers, racemates, pharmaceutically acceptable salts, prodrugs and solvates thereof: R, NH2 2 R2 X HNR21 N3 Rb RN R 2 2

R

12

R

1 1 b Ra o ) 4 N (XII) wherein Ra and Rb are hydrogen or taken together with the carbon atom they attached to form a carbonyl; n is 0-9; X 1 is 0, S or NH; Q, Y, R 2 o, R 21 , R22, R 1 , R2, R3, R4, R, 1 , and R 12 are as previously defined. 20 Representative compounds according to the invention are those selected from the Table A below or its geometric isomers, enantiomers, diastereomers, racemates, pharmaceutically acceptable salts, prodrugs and solvates thereof: 14 WO 2008/033749 PCT/US2007/077977 TABLE A -Compound # Structure 0 HN) 2 1aCi HON 0 N H 0 0 F H " e' N 2 H c 30 HN)1("1 5HO, NH'~AN N 'N N H0) 0 HH 4 H H0 0 5 HO, Q'C] H 0 "1 0 ' N 15F WO 2008/033749 PCT/US2007/077977 10 H HN HO'NO 0 HOO HN 11 HO HO H 12 HON 0 O HN I 12 HO, 0 HN C ON O F 15 HN CI HO HAC 14 H 0 -]N 15 NA'N -'H H XOF HN c 16 H 17 NN H HN CI 18 H 16 WO 2008/033749 PCT/US2007/077977 20 H HN 219H ,N ' 0 HN HO H HH 22 H H 0 230 HN HO, ) , 0N H 24 H H 00 0 H ~ ~ HN, 2 ( 24 H0 1 N . N 0 ~~~N 017N c WO 20081033749 PCTJUS2007/077977 F 270 HN CI H F HN CI 28 H 0 HN ' 2 cI 29 HO, N' N H HN IC&I o" N N 30 0 HN c 0 cr HN N H H N HH H NN HN" 34 0 HN) NJN 34 HI 18' WO 2008/033749 PCT1US2007/077977 350 HN HO, H 36 HHN" HO" N- 0

-"

0 Ii: F 0O HN'aCI 37 HO, N&_., " NN F H N cI H H 38 HO',N NN 0 HN cC 40 HO,N N)::- "'N 0 0 " N"" H HN c 41 H, N N.. , N N H 0 N) HN" 42 HN. HO -" 19 WO 20081033749 PCT/US2007/077977 N 43 HO, N N H N 0 0 N HO, H H 00 F Br NH, 47 HOO Cl H HO HN 46 H HN ,NN) HO 0 HN HN 48 H N9 0.-~.O N~ HH HON 0 HN" .

50 HO, NN 20 WO 20081033749 PCTIUS2007I077977 F HNH F NHb HN" cI 52 ' HN F .NH b HNAXCI 53 0la l H ~NH a 04 H N c l H 0 HN H b HN 56 'NN'lk- ' N H N) HN 57 Br N ii ~N b 0 HN' 58 NN 21 WO 20081033749 PCTJUS2007/077977 59HN' CI HN F 60 I."a~ N H HN NH b HN ' 1 H HO ON jt-O HN< CI 63 H C 0 HO. a 64H N a I 65 H &' H0 N 0 HO, HOH HN cI 66 H0,- C 0 H HN 0 'cO 67 HO ,N -' _ N N 00

N

9 0,-) 22 WO 2008/033749 PCT/US2007/077977 F N H HHC 69 HO' ~ _ N N - " H 01 0O H?-XcI 70 HO. N& , H 0 N -) H H N 71HO-N N H NI 0 HN HN 72 HO I~ ~0 HH H 72 HO'0. _y N N 0 73 N 0 N 0. 74 N N N 0 101) 7 5 H NN C " N N 23 WO 2008/033749 PCT/US2007/077977 76 C H ~ oo HO 78 HC CH H HNI 80 H -N N N OH, 81H H HO N o HCo)0) N N 82 H H N N Q o H 1 co' 83 H N o , NN OH 85 HC)N HN~N OH HN N 86 N~ &N F 0H HC 24 WO 20081033749 PCT/US2007/077977 H HN 88 HO N rN F H HN 89 H 0 0 e 90 HO' ~ NTBr HNF HC 94 H 0 91 0 '.N ' 0 HOH 95 H'ac HN Ml HNI 0 N NC 9 H 0 C ' 8 IN CH H4 99 1c CH 50 0 N N 25 WO 2008/033749 PCT/US2007/077977 o H 100 C H CH o N~~ 0 -~ H 0 O N. 101 OCH CF H2HA 104 2 OH C 103 H 15 O HC 104HNN C CHCF 1065O - ~ . , HN C 0N 107 N N HNN H H C HOO N! 0F 108 c 0 N HN 109 H 0 N~j~ 109 110 N' 111 H UN 0 0 N 26~a WO 2008/033749 PCT/US2007/077977 112 N 113 H 114 HN Cl HO'N5 _ N N H 115 N HO N 116 117 IN HO ' 0& NO HH 119 H 121 O H 0F 0, 1 2 2 H O - N 3 C 0 12127 WO 2008/033749 PCT/US2007/077977 HO '0 a

F

2 'C 123 H HO-N F 1240 I HO 0 125 H IH HO-N 126 N a

N

0 HCF 127N F Ho-N 128 H H Cl 129 HO--N O 0 -::J NNa HO 130 N D-- F HO-NA 131 N 0 132 H HAF 00 133 28 WO 20081033749 PCTLUS2007/077977 HH 134_

HO

136 HOHA F 137 HON 138 H 139 HH 2~ 0 ' Ho,' H f l 140 Hk H 141N N

-

0 I 0 F HO, 142 H - e NN 143 H HN cI HO'N N HN 144 HOC. I, c HI 29 WO 20081033749 PCT/US2007/077977 145 H N'( 146 HO'N N NH 147 H N l H0, N . N 0 N 148 HO,,HN. NN H 149 HO.Ni S N H N 15 H 2 c 152NHZH a H.. ap N H NN 151 HO r- - I N. N 0 0 N 0 WO 20081033749 PCTIUS2007/077977 156 H HN ci Ho' 00 HN C 157 Ho N HN c 1589 0 H c 1 6 11 HA 161 H HN 1 6 2 Ho- o HO_ H 163 H 1651 WO 20081033749 PCT/US2007/077977 H A c 167 0N " HO~N~~N H I 168H Ho H H HO' O O N-) 171 0 HN~c( O -Y O1 171 N aN H H I 0 'N -r 175 H Nc 00" 173 0O~ HN cC HO 0 H 177 M N 0 32 WO 20081033749 PCT/US2007/077977 178 H F N N H 179 HHN C HO o 180 H HNa I F H 181 HN C1 F 1o HN F HR O 181 HO-N MNCO 185N HO MN- C' 182 N NU 18O N Fao H-N ,88F 183 3 o N N

HO

185 N H CI o N ' 188 ' NH, C o J ' 33 WO 2008/033749 PCT/US2007/077977 H F 189 Ho-N 189 H N NHN CI 1900 NF HO F 190 HN CI H N F 191 HN F 194 HD 1950H N 'Or HO 192 N H 1973 H C F HO H,' 194 0 F HO N 195 N~H\4 HNm go HO,~ 199 1993 Hg 34 WO 2008/033749 PCT/US2007/077977 200 ~ 201 -1A HN I 202 N0 N0 ~" Ho H,C.. 203 HN N 0 204 IN 001 205 N HCHN OH 206 HNC NoN 207 HO HW NON 0 HO, N HO N 208 O" 210 N r HO- N 35 WO 2008/033749 PCT/US2007/077977 211 N N C1 212 N HO-N H 212N In a particularly preferred embodiment, the invention relates to geometric isomers, enantiomers, diastereomers, racemates, pharmaceutically acceptable salts, prodrugs and solvates of compounds 12 and 18. The invention further provides methods for the prevention or treatment of diseases 5 or conditions involving aberrant proliferation, differentiation or survival of cells. In one embodiment, the invention further provides for the use of one or more compounds of the invention in the manufacture of a medicament for halting or decreasing diseases involving aberrant proliferation, differentiation, or survival of cells. In preferred embodiments, the disease is cancer. In one embodiment, the invention relates to a method of treating cancer 10 in a subject in need of treatment comprising administering to said subject a therapeutically effective amount of a compound of the invention. The term "cancer" refers to any cancer caused by the proliferation of malignant neoplastic cells, such as tumors, neoplasms, carcinomas, sarcomas, leukemias, lymphomas and the like. For example, cancers include, but are not limited to, mesothelioma, leukemias 15 and lymphomas such as cutaneous T-cell lymphomas (CTCL), noncutaneous peripheral T cell lymphomas, lymphomas associated with human T-cell lymphotrophic virus (HTLV) such as adult T-cell leukemia/lymphoma (ATLL), B-cell lymphoma, acute nonlymphocytic leukemias, chronic lymphocytic leukemia, chronic myelogenous leukemia, acute myelogenous leukemia, lymphomas, and multiple myeloma, non-Hodgkin lymphoma, acute 20 lymphatic leukemia (ALL), chronic lymphatic leukemia (CLL), Hodgkin's lymphoma, Burkitt lymphoma, adult T-cell leukemia lymphoma, acute-myeloid leukemia (AML), chronic myeloid leukemia (CML), or hepatocellular carcinoma. Further examples include myelodisplastic syndrome, childhood solid tumors such as brain tumors, neuroblastoma, retinoblastoma, Wilms' tumor, bone tumors, and soft-tissue sarcomas, common solid tumors 25 of adults such as head and neck cancers (e.g., oral, laryngeal, nasopharyngeal and esophageal), genitourinary cancers (e.g., prostate, bladder, renal, uterine, ovarian, testicular), lung cancer (e.g., small-cell and non small cell), breast cancer, pancreatic cancer, 36 WO 20081033749 PCT/US2007/077977 melanoma and other skin cancers, stomach cancer, brain tumors, tumors related to Gorlin's syndrome (e.g., medulloblastoma, meningioma, etc.), and liver cancer. Additional exemplary forms of cancer which may be treated by the subject compounds include, but are not limited to, cancer of skeletal or smooth muscle, stomach cancer, cancer of the small 5 intestine, rectum carcinoma, cancer of the salivary gland, endometrial cancer, adrenal cancer, anal cancer, rectal cancer, parathyroid cancer, and pituitary cancer. Additional cancers that the compounds described herein may be useful in preventing, treating and studying are, for example, colon carcinoma, familiary adenomatous polyposis carcinoma and hereditary non-polyposis colorectal cancer, or melanoma. Further, 10 cancers include, but are not limited to, labial carcinoma, larynx carcinoma, hypopharynx carcinoma, tongue carcinoma, salivary gland carcinoma, gastric carcinoma, adenocarcinoma, thyroid cancer (medullary and papillary thyroid carcinoma), renal carcinoma, kidney parenchyma carcinoma, cervix carcinoma, uterine corpus carcinoma, endometrium carcinoma, chorion carcinoma, testis carcinoma, urinary carcinoma, 15 melanoma, brain tumors such as glioblastoma, astrocytoma, meningioma, medulloblastoma and peripheral neuroectodermal tumors, gall bladder carcinoma, bronchial carcinoma, multiple myeloma, basalioma, teratoma, retinoblastoma, choroidea melanoma, seminoma, rhabdomyosarcoma, craniopharyngeoma, osteosarcoma, chondrosarcoma, myosarcoma, liposarcoma, fibrosarcoma, Ewing sarcoma, and plasmocytoma. In one aspect of the 20 invention, the present invention provides for the use of one or more compounds of the invention in the manufacture of a medicament for the treatment of cancer. In one embodiment, the present invention includes the use of one or more compounds of the invention in the manufacture of a medicament that prevents further aberrant proliferation, differentiation, or survival of cells. For example, compounds of the 25 invention may be useful in preventing tumors from increasing in size or from reaching a metastatic state. The subject compounds may be administered to halt the progression or advancement of cancer or to induce tumor apoptosis or to inhibit tumor angiogenesis. In addition, the instant invention includes use of the subject compounds to prevent a recurrence of cancer. 30 This invention further embraces the treatment or prevention of cell proliferative disorders such as hyperplasias, dysplasias and pre-cancerous lesions. Dysplasia is the earliest form of pre-cancerous lesion recognizable in a biopsy by a pathologist. The subject compounds may be administered for the purpose of preventing said hyperplasias, dysplasias 37 WO 2008/033749 PCT/US2007/077977 or pre-cancerous lesions from continuing to expand or from becoming cancerous. Examples of pre-cancerous lesions may occur in skin, esophageal tissue, breast and cervical intra epithelial tissue. "Combination therapy" includes the administration of the subject compounds in 5 further combination with other biologically active ingredients (such as, but not limited to, a second and different antineoplastic agent) and non-drug therapies (such as, but not limited to, surgery or radiation treatment). For instance, the compounds of the invention can be used in combination with other pharmaceutically active compounds, preferably compounds that are able to enhance the effect of the compounds of the invention. The compounds of the 10 invention can be administered simultaneously (as a single preparation or separate preparation) or sequentially to the other drug therapy. In general, a combination therapy envisions administration of two or more drugs during a single cycle or course of therapy. In one aspect of the invention, the subject compounds may be administered in combination with one or more separate agents that modulate protein kinases involved in 15 various disease states. Examples of such kinases may include, but are not limited to: serine/threonine specific kinases, receptor tyrosine specific kinases and non-receptor tyrosine specific kinases. Serine/threonine kinases include mitogen activated protein kinases (MAPK), meiosis specific kinase (MEK), RAF and aurora kinase. Examples of receptor kinase families include epidermal growth factor receptor (EGFR) (e.g. HER2/neu, 20 HER3, HER4, ErbB, ErbB2, ErbB3, ErbB4, Xmrk, DER, Let23); fibroblast growth factor (FGF) receptor (e.g. FGF-R1,GFF-R2/BEK/CEK3, FGF-R3/CEK2, FGF-R4/TKF, KGF-R); hepatocyte growth/scatter factor receptor (HGFR) (e.g, MET, RON, SEA, SEX); insulin receptor (e.g. IGFI-R); Eph (e.g. CEK5, CEK8, EBK, ECK, EEK, EHK-1, EHK-2, ELK, EPH, ERK, HEK, MDK2, MDK5, SEK); Axl (e.g. Mer/Nyk, Rse); RET; and platelet 25 derived growth factor receptor (PDGFR) (e.g. PDGFa-R, PDG3-R, CSF1-R/FMS,

SCF

R/C-KIT, VEGF-R/FLT, NEKIFLKI, FLT3/FLK2/STK-1). Non-receptor tyrosine kinase families include, but are not limited to, BCR-ABL (e.g. p 4 3 bl, ARG); BTK (e.g. ITK/EMT, TEC); CSK, FAK, FPS, JAK, SRC, BMX, FER, CDK and SYK. In another aspect of the invention, the subject compounds may be administered in 30 combination with one or more agents that modulate non-kinase biological targets or processes. Such targets include histone deacetylases (HDAC), DNA methyltransferase (DNMT), heat shock proteins (e.g. HSP90), and proteosomes. 38 WO 20081033749 PCT/US2007/077977 In a preferred embodiment, subject compounds may be combined with antineoplastic agents (e.g. small molecules, monoclonal antibodies, antisense RNA, and fusion proteins) that inhibit one or more biological targets such as Zolinza, Tarceva, Iressa, Tykerb, Gleevec, Sutent, Sprycel, Nexavar, Sorafinib, CNF2024, RG108, BMS387032, 5 Affinitak, Avastin, Herceptin, Erbitux, AG24322, PD325901, ZD6474, PD184322, Obatodax, ABT737 and AEE788. Such combinations may enhance therapeutic efficacy over efficacy achieved by any of the agents alone and may prevent or delay the appearance of resistant mutational variants. In certain preferred embodiments, the compounds of the invention are administered 10 in combination with a chemotherapeutic agent. Chemotherapeutic agents encompass a wide range of therapeutic treatments in the field of oncology. These agents are administered at various stages of the disease for the purposes of shrinking tumors, destroying remaining cancer cells left over after surgery, inducing remission, maintaining remission and/or alleviating symptoms relating to the cancer or its treatment. Examples of such agents 15 include, but are not limited to, alkylating agents such as mustard gas derivatives (Mechlorethamine, cylophosphamide, chlorambucil, melphalan, ifosfamide), ethylenimines (thiotepa, hexamethylmelanine), Alkylsulfonates (Busulfan), Hydrazines and Triazines (Altretamine, Procarbazine, Dacarbazine and Temozolomide), Nitrosoureas (Carmustine, Lomustine and Streptozocin), Ifosfamide and metal salts (Carboplatin, Cisplatin, and 20 Oxaliplatin); plant alkaloids such as Podophyllotoxins (Etoposide and Tenisopide), Taxanes (Paclitaxel and Docetaxel), Vinca alkaloids (Vincristine, Vinblastine, Vindesine and Vinorelbine), and Camptothecan analogs (Irinotecan and Topotecan); anti-tumor antibiotics such as Chromomycins (Dactinomycin and Plicamycin), Anthracyclines (Doxorubicin, Daunorubicin, Epirubicin, Mitoxantrone, Valrubicin and Idarubicin), and miscellaneous 25 antibiotics such as Mitomycin, Actinomycin and Bleomycin; anti-metabolites such as folic acid antagonists (Methotrexate, Pemetrexed, Raltitrexed, Aminopterin), pyrimidine antagonists (5-Fluorouracil, Floxuridine, Cytarabine, Capecitabine, and Gemcitabine), purine antagonists (6-Mercaptopurine and 6-Thioguanine) and adenosine deaminase inhibitors (Cladribine, Fludarabine, Mercaptopurine, Clofarabine, Thioguanine, 30 Nelarabine and Pentostatin); topoisomerase inhibitors such as topoisomerase I inhibitors (Ironotecan, topotecan) and topoisomerase II inhibitors (Amsacrine, etoposide, etoposide phosphate, teniposide); monoclonal antibodies (Alemtuzumab, Gemtuzumab ozogamicin, Rituximab, Trastuzumab, Ibritumomab Tioxetan, Cetuximab, Panitumumab, Tositumomab, 39 WO 2008/033749 PCT/US2007/077977 Bevacizumab); and miscellaneous anti-neoplastics such as ribonucleotide reductase inhibitors (Hydroxyurea); adrenocortical steroid inhibitor (Mitotane); enzymes (Asparaginase and Pegaspargase); anti-microtubule agents (Estramustine); and retinoids (Bexarotene, Isotretinoin, Tretinoin (ATRA). 5 In certain preferred embodiments, the compounds of the invention are administered in combination with a chemoprotective agent. Chemoprotective agents act to protect the body or minimize the side effects of chemotherapy. Examples of such agents include, but are not limited to, amfostine, mesna, and dexrazoxane. In one aspect of the invention, the subject compounds are administered in 10 combination with radiation therapy. Radiation is commonly delivered internally (implantation of radioactive material near cancer site) or externally from a machine that employs photon (x-ray or gamma-ray) or particle radiation. Where the combination therapy further comprises radiation treatment, the radiation treatment may be conducted at any suitable time so long as a beneficial effect from the co-action of the combination of the 15 therapeutic agents and radiation treatment is achieved. For example, in appropriate cases, the beneficial effect is still achieved when the radiation treatment is temporally removed from the administration of the therapeutic agents, perhaps by days or even weeks. It will be appreciated that compounds of the invention can be used in combination with an immunotherapeutic agent. One form of immunotherapy is the generation of an 20 active systemic tumor-specific immune response of host origin by administering a vaccine composition at a site distant from the tumor. Various types of vaccines have been proposed, including isolated tumor-antigen vaccines and anti-idiotype vaccines. Another approach is to use tumor cells from the subject to be treated, or a derivative of such cells (reviewed by Schirrmacher et al. (1995) J. Cancer Res. Clin. Oncol. 121:487). In U.S. Pat. No. 5,484,596, 25 Hanna Jr. et al. claim a method for treating a resectable carcinoma to prevent recurrence or metastases, comprising surgically removing the tumor, dispersing the cells with collagenase, irradiating the cells, and vaccinating the patient with at least three consecutive doses of about 107 cells. It will be appreciated that the compounds of the invention may advantageously be 30 used in conjunction with one or more adjunctive therapeutic agents. Examples of suitable agents for adjunctive therapy include a 5HT 1 agonist, such as a triptan (e.g. sumatriptan or naratriptan); an adenosine Al agonist; an EP ligand; an NMDA modulator, such as a glycine antagonist; a sodium channel blocker (e.g. lamotrigine); a substance P antagonist 40 WO 2008/033749 PCT/US2007/077977 (e.g. an NK 1 antagonist); a cannabinoid; acetaminophen or phenacetin; a 5-lipoxygenase inhibitor; a leukotriene receptor antagonist; a DMARD (e.g. methotrexate); gabapentin and related compounds; a tricyclic antidepressant (e.g. amitryptilline); a neurone stabilising antiepileptic drug; a mono-aminergic uptake inhibitor (e.g. venlafaxine); a matrix 5 metalloproteinase inhibitor; a nitric oxide synthase (NOS) inhibitor, such as an iNOS or an nNOS inhibitor; an inhibitor of the release, or action, of tumour necrosis factor .alpha.; an antibody therapy, such as a monoclonal antibody therapy; an antiviral agent, such as a nucleoside inhibitor (e.g. lamivudine) or an immune system modulator (e.g. interferon); an opioid analgesic; a local anaesthetic; a stimulant, including caffeine; an H2-antagonist (e.g. 10 ranitidine); a proton pump inhibitor (e.g. omeprazole); an antacid (e.g. aluminium or magnesium hydroxide; an antiflatulent (e.g. simethicone); a decongestant (e.g. phenylephrine, phenylpropanolamine, pseudoephedrine, oxymetazoline, epinephrine, naphazoline, xylometazoline, propylhexedrine, or levo-desoxyephedrine); an antitussive (e.g. codeine, hydrocodone, carmiphen, carbetapentane, or dextramethorphan); a diuretic; or 15 a sedating or non-sedating antihistamine. Matrix metalloproteinases (MMPs) are a family of zinc-dependent neutral endopeptidases collectively capable of degrading essentially all matrix components. Over 20 MMP modulating agents are in pharmaceutical develop, almost half of which are indicated for cancer. The University of Toronto researchers have reported that HDACs 20 regulate MMP expression and activity in 3T3 cells. In particular, inhibition of HDAC by trichostatin A (TSA), which has been shown to prevent tumorigenesis and metastasis, decreases mRNA as well as zymographic activity of gelatinase A (MMP2; Type IV collagenase), a matrix metalloproteinase, which is itself, implicated in tumorigenesis and metastasis (Ailenberg M., Silverman M., Biochem Biophys Res Commun. 2002, 298:110 25 115). Another recent article that discusses the relationship of HDAC and MMPs can be found in Young D.A., et al., Arthritis Research & Therapy, 2005, 7: 503. Furthermore, the commonality between HDAC and MMPs inhibitors is their zinc-binding functionality. Therefore, in one aspect of the invention, compounds of the invention can be used as MMP inhibitors and may be of use in the treatment of disorders relating to or associated with 30 dysregulation of MMP. The overexpression and activation of MMPs are known to induce tissue destruction and are also associated with a number of specific diseases including rheumatoid arthritis, periodontal disease, cancer and atherosclerosis. 41 WO 20081033749 PCT/US2007/077977 The compounds may also be used in the treatment of a disorder involving, relating to or, associated with dysregulation of histone deacetylase (HDAC). There are a number of disorders that have been implicated by or known to be mediated at least in part by -IDAC activity, where HDAC activity is known to play a role in triggering disease onset, or whose 5 symptoms are known or have been shown to be alleviated by HDAC inhibitors. Disorders of this type that would be expected to be amenable to treatment with the compounds of the invention include the following but not limited to: Anti-proliferative disorders (e.g. cancers); Neurodegenerative diseases including Huntington's Disease, Polyglutamine disease, Parkinson's Disease, Alzheimer's Disease, Seizures, Striatonigral degeneration, 10 Progressive supranuclear palsy, Torsion dystonia, Spasmodic torticollis and dyskinesis, Familial tremor, Gilles de la Tourette syndrome, Diffuse Lewy body disease, Progressive supranuclear palsy, Pick's disease, intracerebral hemorrhage, Primary lateral sclerosis, Spinal muscular atrophy, Amyotrophic lateral sclerosis, Hypertrophic interstitial polyneuropathy, Retinitis pigmentosa, Hereditary optic atrophy, Hereditary spastic 15 paraplegia, Progressive ataxia and Shy-Drager syndrome; Metabolic diseases including Type 2 diabetes; Degenerative Diseases of the Eye including Glaucoma, Age-related macular degeneration, Rubeotic glaucoma; Inflammatory diseases and/or Immune system disorders including Rheumatoid Arthritis (RA), Osteoarthritis, Juvenile chronic arthritis, Graft versus Host disease, Psoriasis, Asthma, Spondyloarthropathy, Crohn's Disease, 20 inflammatory bowel disease Colitis Ulcerosa, Alcoholic hepatitis, Diabetes, Sjoegrens's syndrome, Multiple Sclerosis, Ankylosing spondylitis, Membranous glomerulopathy, Discogenic pain, Systemic Lupus Erythematosus; Disease involving angiogenesis including cancer, psoriasis, rheumatoid arthritis; Psychological disorders including bipolar disease, schizophrenia, mania, depression and dementia; Cardiovascular Diseases including heart 25 failure, restenosis and arteriosclerosis; Fibrotic diseases including liver fibrosis, cystic fibrosis and angiofibroma; Infectious diseases including Fungal infections, such as Candida Albicans, Bacterial infections, Viral infections, such as Herpes Simplex, Protozoal infections, such as Malaria, Leishmania infection, Trypanosoma brucei infection, Toxoplasmosis and coccidlosis and Haematopoietic disorders including thalassemia, anemia 30 and sickle cell anemia. In one embodiment, compounds of the invention can be used to induce or inhibit apoptosis, a physiological cell death process critical for normal development and homeostasis. Alterations of apoptotic pathways contribute to the pathogenesis of a variety 42 WO 2008/033749 PCT/US2007/077977 of human diseases. Compounds of the invention, as modulators of apoptosis, will be useful in the treatment of a variety of human diseases with abberations in apoptosis including cancer (particularly, but not limited to, follicular lymphomas, carcinomas with p53 mutations, hormone dependent tumors of the breast, prostate and ovary, and precancerous 5 lesions such as familial adenomatous polyposis), viral infections (including, but not limited to, herpesvirus, poxvirus, Epstein-Barr virus, Sindbis virus and adenovirus), autoimmune diseases (including, but not limited to, systemic lupus, erythematosus, immune mediated glomerulonephritis, rheumatoid arthritis, psoriasis, inflammatory bowel diseases, and autoimmune diabetes mellitus), neurodegenerative disorders (including, but not limited to, 10 Alzheimer's disease, AIDS-related dementia, Parkinson's disease, amyotrophic lateral sclerosis, retinitis pigmentosa, spinal muscular atrophy and cerebellar degeneration), AIDS, myelodysplastic syndromes, aplastic anemia, ischemic injury associated myocardial infarctions, stroke and reperfusion injury, arrhythmia, atherosclerosis, toxin-induced or alcohol induced liver diseases, hematological diseases (including, but not limited to, chronic 15 anemia and aplastic anemia), degenerative diseases of the musculoskeletal system (including, but not limited to, osteoporosis and arthritis), aspirin-sensitive rhinosinusitis, cystic fibrosis, multiple sclerosis, kidney diseases, and cancer pain. In one aspect, the invention provides the use of compounds of the invention for the treatment and/or prevention of immune response or immune-mediated responses and 20 diseases, such as the prevention or treatment of rejection following transplantation of synthetic or organic grafting materials, cells, organs or tissue to replace all or part of the function of tissues, such as heart, kidney, liver, bone marrow, skin, cornea, vessels, lung, pancreas, intestine, limb, muscle, nerve tissue, duodenum, small-bowel, pancreatic-islet cell, including xeno-transplants, etc.; to treat or prevent graft-versus-host disease, 25 autoimmune diseases, such as rheumatoid arthritis, systemic lupus erythematosus, thyroiditis, Hashimoto's thyroiditis, multiple sclerosis, myasthenia gravis, type I diabetes uveitis, juvenile-onset or recent-onset diabetes mellitus, uveitis, Graves disease, psoriasis, atopic dermatitis, Crohn's disease, ulcerative colitis, vasculitis, auto-antibody mediated diseases, aplastic anemia, Evan's syndrome, autoimmune hemolytic anemia, and the like; 30 and further to treat infectious diseases causing aberrant immune response and/or activation, such as traumatic or pathogen induced immune disregulation, including for example, that which are caused by hepatitis B and C infections, HIV, staphylococcus aureus infection, viral encephalitis, sepsis, parasitic diseases wherein damage is induced by an inflammatory 43 WO 2008/033749 PCT/US2007/077977 response (e.g., leprosy); and to prevent or treat circulatory diseases, such as arteriosclerosis, atherosclerosis, vasculitis, polyarteritis nodosa and myocarditis. In addition, the present invention may be used to prevent/suppress an immune response associated with a gene therapy treatment, such as the introduction of foreign genes into autologous cells and 5 expression of the encoded product. Thus in one embodiment, the invention relates to a method of treating an immune response disease or disorder or an immune-mediated response or disorder in a subject in need of treatment comprising administering to said subject a therapeutically effective amount of a compound of the invention. In one aspect, the invention provides the use of compounds of the invention in the 10 treatment of a variety of neurodegenerative diseases, a non-exhaustive list of which includes: I. Disorders characterized by progressive dementia in the absence of other prominent neurologic signs, such as Alzheimer's disease; Senile dementia of the Alzheimer type; and Pick's disease (lobar atrophy); II. Syndromes combining progressive dementia with other prominent neurologic abnormalities such as A) syndromes appearing mainly in 15 adults (e.g., Huntington's disease, Multiple system atrophy combining dementia with ataxia and/or manifestations of Parkinson's disease, Progressive supranuclear palsy (Steel Richardson-Olszewski), diffuse Lewy body disease, and corticodentatonigral degeneration); and B) syndromes appearing mainly in children or young adults (e.g., Hallervorden-Spatz disease and progressive familial myoclonic epilepsy); III. Syndromes of gradually 20 developing abnormalities of posture and movement such as paralysis agitans (Parkinson's disease), striatonigral degeneration, progressive supranuclear palsy, torsion dystonia (torsion spasm; dystonia musculorum deformans), spasmodic torticollis and other dyskinesis, familial tremor, and Gilles de la Tourette syndrome; IV. Syndromes of progressive ataxia such as cerebellar degenerations (e.g., cerebellar cortical degeneration 25 and olivopontocerebellar atrophy (OPCA)); and spinocerebellar degeneration (Friedreich's atazia and related disorders); V. Syndrome of central autonomic nervous system failure (Shy-Drager syndrome); VI. Syndromes of muscular weakness and wasting without sensory changes (motomeuron disease such as amyotrophic lateral sclerosis, spinal muscular atrophy (e.g., infantile spinal muscular atrophy (Werdnig-Hoffman), juvenile spinal 30 muscular atrophy (Wohlfart-Kugelberg-Welander) and other forms of familial spinal muscular atrophy), primary lateral sclerosis, and hereditary spastic paraplegia; VII. Syndromes combining muscular weakness and wasting with sensory changes (progressive neural muscular atrophy; chronic familial polyneuropathies) such as peroneal muscular 44 WO 2008/033749 PCT/US20071077977 atrophy (Charcot-Marie-Tooth), hypertrophic interstitial polyneuropathy (Dejerine-Sottas), and miscellaneous forms of chronic progressive neuropathy; VIII Syndromes of progressive visual loss such as pigmentary degeneration of the retina (retinitis pigmentosa), and hereditary optic atrophy (Leber's disease). Furthermore, compounds of the invention can be 5 implicated in chromatin remodeling. The invention encompasses pharmaceutical compositions comprising pharmaceutically acceptable salts of or complexes with the compounds of the invention as described above. Examples of suitable salts or complexes include but are not limited to the sodium hydrochloride, citrate or tartrate salt, preferably the tartrate salt. The invention also 10 encompasses pharmaceutical compositions comprising solvates or hydrates of the compounds of the invention. The term "hydrate" includes but is not limited to hemihydrate, monohydrate, dihydrate, trihydrate and the like. In yet another embodiment, the invention relates to a tartrate salt of or complex with compound 12. In an additional embodiment, the invention is an L-tartrate salt of or complex with compound 12. 15 The tartrate salts or complexes of the invention include the L-tartrate (L-(R,R)-(+) tartrate), D-tartrate (D-((S,S)-(-)-tartrate), D,L-tartrate and meso-tartrate (2R, 3S-tartrate) salts. In another embodiment, the tartrate salt is an anhydrous salt. In a further embodiment, the tartrate salt is a hydrate salt. The term "hydrate" includes but is not limited to hemihydrate, monohydrate, dehydrate, trihydrate and the like. 20 In a further embodiment, the invention pertains to an L-tartrate salt of or complex with a compound represented by compounds of the invention. In yet another embodiment, the invention is an L-tartrate salt of or complex with a compound selected from the compounds shown in Table A above. In an additional embodiment, the invention is an L tartrate salt of or complex with a compound selected from the group consisting of 25 compound 12 and compound 18. In a further embodiment, the invention is an L-tartrate salt of or complex with compound 12. In an additional embodiment, the invention pertains to a D-tartrate salt of or complex with a compound represented by Formulae 1, 11, III or IV. In yet another embodiment, the invention is a D-tartrate salt of or complex with a compound selected from 30 the compounds shown in Table A above. In an additional embodiment, the invention is a D tartrate salt of or complex with a compound selected from the group consisting of compound 12 and compound 18. In a further embodiment, the invention is a D-tartrate salt of or complex with compound 12. 45 WO 20081033749 PCT/US2007/077977 In a further embodiment, the invention pertains to an D,L-tartrate salt of or complex with a compound represented by compounds of the invention. In yet another embodiment, the invention is an D,L-tartrate salt of or complex with a compound selected from the compounds shown in Table A above. In an additional embodiment, the invention is an D,L 5 tartrate salt of or complex with a compound selected from the group consisting of compound 12 and compound 18. In a further embodiment, the invention is an D,L-tartrate salt of or complex with compound 12. In yet another embodiment, the invention is a meso-tartrate salt of or complex with a compound represented by compounds of the invention. In yet another embodiment, the 10 invention is a meso-tartrate salt of or complex with a compound selected from the compounds shown in Table A above. In an additional embodiment, the invention is an meso-tartrate salt of or complex with a compound selected from the group consisting of compound 12 and compound 18. In a further embodiment, the invention is a meso-tartrate salt of or complex with compound 12. 15 In one embodiment, the invention pertains to a pharmaceutical composition comprising a tartrate salt of or complex with the invention at a therapeutically effective amount and a pharmaceutically acceptable carrier. In another embodiment, the pharmaceutical composition is a liquid formulation. In yet another embodiment, the pharmaceutical composition is an aqueous formulation. In yet another embodiment, the 20 liquid formulation is a parenteral formulation. In yet another embodiment, the formulation is an intravenous formulation. The invention further encompasses pharmaceutical compositions comprising any solid or liquid physical form of the compound of the invention. The particles may be micronized, or may be agglomerated, particulate granules, powders, oils, oily suspensions or any other form of solid or liquid physical form. 25 The compounds of the invention, and derivatives, fragments, analogs, homologs, pharmaceutically acceptable salts or hydrate thereof can be incorporated into pharmaceutical compositions suitable for administration, together with a pharmaceutically acceptable carrier or excipient. Such compositions typically comprise a therapeutically effective amount of any of the compounds above, and a pharmaceutically acceptable carrier. 30 Preferably, the effective amount when treating cancer is an amount effective to selectively induce terminal differentiation of suitable neoplastic cells and less than an amount which causes toxicity in a patient. 46 WO 2008/033749 PCT/US2007/077977 Compounds of the invention may be administered by any suitable means, including, without limitation, parenteral, intravenous, intramuscular, subcutaneous, implantation, oral, sublingual, buccal, nasal, pulmonary, transdermal, topical, vaginal, rectal, and transmucosal administrations or the like. Topical administration can also involve the use of transdermal 5 administration such as transdermal patches or iontophoresis devices. Pharmaceutical preparations include a solid, semisolid or liquid preparation (tablet, pellet, troche, capsule, suppository, cream, ointment, aerosol, powder, liquid, emulsion, suspension, syrup, injection etc.) containing a compound of the invention as an active ingredient, which is suitable for selected mode of administration. In one embodiment, the pharmaceutical 10 compositions are administered orally, and are thus formulated in a form suitable for oral administration, i.e., as a solid or a liquid preparation. Suitable solid oral formulations include tablets, capsules, pills, granules, pellets, sachets and effervescent, powders, and the like. Suitable liquid oral formulations include solutions, suspensions, dispersions, emulsions, oils and the like. In one embodiment of the present invention, the composition is 15 formulated in a capsule. In accordance with this embodiment, the compositions of the present invention comprise in addition to the active compound and the inert carrier or diluent, a hard gelatin capsule. Any inert excipient that is commonly used as a carrier or diluent may be used in the formulations of the present invention, such as for example, a gum, a starch, a sugar, a 20 cellulosic material, an acrylate, or mixtures thereof. A preferred diluent is microcrystalline cellulose. The compositions may further comprise a disintegrating agent (e.g., croscarmellose sodium) and a lubricant (e.g., magnesium stearate), and may additionally comprise one or more additives selected from a binder, a buffer, a protease inhibitor, a surfactant, a solubilizing agent, a plasticizer, an emulsifier, a stabilizing agent, a viscosity 25 increasing agent, a sweetener, a film forming agent, or any combination thereof. Furthermore, the compositions of the present invention may be in the form of controlled release or immediate release formulations. For liquid formulations, pharmaceutically acceptable carriers may be aqueous or non-aqueous solutions, suspensions, emulsions or oils. Examples of non-aqueous solvents 30 are propylene glycol, polyethylene glycol, and injectable organic esters such as ethyl oleate. Aqueous carriers include water, alcoholic/aqueous solutions, emulsions or suspensions, including saline and buffered media. Examples of oils are those of petroleum, animal, vegetable, or synthetic origin, for example, peanut oil, soybean oil, mineral oil, olive oil, 47 WO 20081033749 PCT/US2007/077977 sunflower oil, and fish-liver oil. Solutions or suspensions can also include the following components: a sterile diluent such as water for injection, saline solution, fixed oils, polyethylene glycols, glycerine, propylene glycol or other synthetic solvents; antibacterial agents such as benzyl alcohol or methyl parabens; antioxidants such as ascorbic acid or 5 sodium bisulfite; chelating agents such as ethylenediaminetetraacetic acid (EDTA); buffers such as acetates, citrates or phosphates, and agents for the adjustment of tonicity such as sodium chloride or dextrose. The pH can be adjusted with acids or bases, such as hydrochloric acid or sodium hydroxide. In addition, the compositions may further comprise binders (e.g., acacia, cornstarch, 10 gelatin, carbomer, ethyl cellulose, guar gum, hydroxypropyl cellulose, hydroxypropyl methyl cellulose, povidone), disintegrating agents (e.g., cornstarch, potato starch, alginic acid, silicon dioxide, croscarmellose sodium, crospovidone, guar gum, sodium starch glycolate, Primogel), buffers (e.g., tris-HCI., acetate, phosphate) of various pH and ionic strength, additives such as albumin or gelatin to prevent absorption to surfaces, detergents 15 (e.g., Tween 20, Tween 80, Pluronic F68, bile acid salts), protease inhibitors, surfactants (e.g., sodium lauryl sulfate), permeation enhancers, solubilizing agents (e.g., glycerol, polyethylene glycerol), a glidant (e.g., colloidal silicon dioxide), anti-oxidants (e.g., ascorbic acid, sodium metabisulfite, butylated hydroxyanisole), stabilizers (e.g., hydroxypropyl cellulose, hydroxypropylmethyl cellulose), viscosity increasing agents (e.g., 20 carbomer, colloidal silicon dioxide, ethyl cellulose, guar gum), sweeteners (e.g., sucrose, aspartame, citric acid), flavoring agents (e.g., peppermint, methyl salicylate, or orange flavoring), preservatives (e.g., Thimerosal, benzyl alcohol, parabens), lubricants (e.g., stearic acid, magnesium stearate, polyethylene glycol, sodium lauryl sulfate), flow-aids (e.g., colloidal silicon dioxide), plasticizers (e.g., diethyl phthalate, triethyl citrate), 25 emulsifiers (e.g., carbomer, hydroxypropyl cellulose, sodium lauryl sulfate), polymer coatings (e.g., poloxamers or poloxamines), coating and film forming agents (e.g., ethyl cellulose, acrylates, polymethacrylates) and/or adjuvants. In one embodiment, the active compounds are prepared with carriers that will protect the compound against rapid elimination from the body, such as a controlled release 30 formulation, including implants and microencapsulated delivery systems. Biodegradable, biocompatible polymers can be used, such as ethylene vinyl acetate, polyanhydrides, polyglycolic acid, collagen, polyorthoesters, and polylactic acid. Methods for preparation of such formulations will be apparent to those skilled in the art. The materials can also be 48 WO 2008/033749 PCT/US2007/077977 obtained commercially from Alza Corporation and Nova Pharmaceuticals, Inc. Liposomal suspensions (including liposomes targeted to infected cells with monoclonal antibodies to viral antigens) can also be used as pharmaceutically acceptable carriers. These can be prepared according to methods known to those skilled in the art, for example, as described 5 in U.S. Pat No. 4,522,811. It is especially advantageous to formulate oral compositions in dosage unit form for ease of administration and uniformity of dosage. Dosage unit form as used herein refers to physically discrete units suited as unitary dosages for the subject to be treated; each unit containing a predetermined quantity of active compound calculated to produce the desired 10 therapeutic effect in association with the required pharmaceutical carrier. The specification for the dosage unit forms of the invention are dictated by and directly dependent on the unique characteristics of the active compound and the particular therapeutic effect to be achieved, and the limitations inherent in the art of compounding such an active compound for the treatment of individuals. 15 In one preferred embodiment, the compound can be formulated in an aqueous solution for intravenous injection. In one embodiment, solubilizing agents can be suitably employed. A particularly preferred solubilizing agent includes cyclodextrins and modified cyclodextrins, such as sulfonic acid substituted p-cyclodextrin derivative or salt thereof. In a particularly preferred embodiment, the inclusion complex comprises a 20 cyclodextrin and a compound selected from the group consisting of compound 12 and compound 18 or a geometric isomer, enantiomer, diastereomer, racemate, pharmaceutically acceptable salt, prodrug or solvate thereof. Cyclodextrins are cyclic oligomers of dextrose with a truncated cone structure consisting of a hydrophilic exterior and a hydrophobic interior cavity. A cyclodextrin can 25 form an inclusion complex with a guest molecule by complexing with all or a portion of a hydrophobic guest molecule within its cavity (for example, as described in U.S. Patent No. 4,727,064, the contents of which are herein incorporated by reference). The size of the cavity is determined by the number of glucopyranose units in the cyclodextrin. Alpha- (a), beta- (B) and gamma- (y) cyclodextrins are the most common cyclodextrins and possess six, 30 seven and eight glucopyranose units, respectively. Because natural cyclodextrins have relatively low aqueous solubility and are associated with toxicity, chemically modified cyclodextrin derivatives have been developed to overcome these limitations. Such cyclodextrin derivatives typically possess a chemical modification at one or more of the 2, 49 WO 2008/033749 PCT/US2007/077977 3, or 6 position hydroxyl groups. Cyclodextrin derivatives have, for example, been described in U.S. Pat. Nos. 5,134,127, 5,376,645, 5,571,534, 5,874,418, 6,046,177 and 6,133,248, the contents of which are herein incorporated by reference and made a part hereof. As used herein, the terms "cyclodextrin," "a -cyclodextrin," "P-cyclodextrin and "y 5 cyclodextrin" are intended to encompass unmodified cyclodextrins as well as chemically modified derivatives thereof. The compositions of the invention comprise an inclusion complex of a cyclodextrin and a compound of Formulae (I), (II), (III) or (IV). In yet another embodiment, the composition comprises a therapeutically effective concentration of a compound of Formulae 10 (I), (II), (III) or (IV). In a further embodiment, the composition further comprises a pharmaceutically acceptable excipient or carrier. In one embodiment of the invention, the composition comprises a cyclodextrin selected from the group consisting of c-cyclodextrin, 1-cyclodextrin and y-cyclodextrin. In yet another embodiment, the cyclodextrin is a 0-cyclodextrin and y-cyclodextrin. In an 15 additional embodiment, the cyclodextrin is a p-cyclodextrin. In a further embodiment, the cyclodextrin is selected from the group consisting of a 2 -hydroxypropyl-p-cyclodextrin (Pitha et al, J Pharm Sci, 84 (8), 927-32 (1995)) and sulfobutyl derivatized-0-cyclodextrin (described, for example, in U.S. Patent Nos. 5,134,127, 5,376,645, 5,874,418, 6,046,177 and 6,133,248). In another embodiment, the cyclodextrin is a sulfobutyl derivatized-p 20 cyclodextrin. One such sulfobutyl derivatized--cyclodextrin is sulfobutylether-7-p cyclodextrin and is sold by CyDex, Inc. under the tradename CAPTISOL@. In yet another embodiment of the invention, the cyclodextrin is sulfobutylether-7-j-cyclodextrin. The cyclodextrin may be included in an amount that increases the solubility of the active compound in the composition. In one embodiment, the amount of cyclodextrin 25 included within the composition is the minimal amount needed to solubilize the drug in the composition. In yet another embodiment, the amount of cyclodextrin included within the composition is within about 5% of the minimal amount needed to solubilize the drug. In a further embodiment, the composition is a parenteral formulation and the amount of cyclodextrin included within the formulation is the minimal amount of cyclodextrin needed 30 to solubilize the drug. In order to determine the minimum amount of cyclodextrin needed to solubilize a compound encompassed by Formulae I-IV, a plot of the compound's solubility versus cyclodextrin concentration can be carried out. By interpolating or extrapolating from 50 WO 20081033749 PCT/US2007/077977 the plot, a composition can be prepared that contains the minimum amount of cyclodextrin needed to dissolve the desired concentration of the active compound. In one embodiment, the composition comprises at least about 0.5 or 1% (weight/volume) of a cyclodextrin. In another embodiment, the composition comprises at 5 least about 5% of a cyclodextrin. In yet another embodiment, the composition comprises at least about 15% of a cyclodextrin. In a further embodiment, the composition comprises from about 0.5 to about 50% of a cyclodextrin. In yet another embodiment, the composition comprises from about 0.5% to about 40% of a cyclodextrin. In another embodiment, the composition comprises about 0.5% to about 35% of a cycloextrin. In yet another 10 embodiment, the composition comprises about 30% of a cyclodextrin. In another embodiment, the composition comprises at least about 0.5 or 1% (weight/volume) of a sulfobutyl derivatized-p-cyclodextrin. In another embodiment, the composition comprises at least about 5% of a sulfobutyl derivatized-p-cyclodextrin. In yet another embodiment, the composition comprises at least about 15% of a sulfobutyl 15 derivatized-p-cyclodextrin. In a further embodiment, the composition comprises from about 0.5 to about 50% of a sulfobutyl derivatized-p-cyclodextrin. In yet another embodiment, the composition comprises from about 0.5% to about 40% of a sulfobutyl derivatized-p-cyclodextrin. In another embodiment, the composition comprises about 0.5% to about 35% of a sulfobutyl derivatized-p-cyclodextrin. In yet another embodiment, the 20 composition comprises about 30% of a sulfobutyl derivatized-P-cyclodextrin. In one embodiment, the composition comprises at least about 0.5 or 1% (weight/volume) CAPTISOL. In another embodiment, the composition comprises at least about 5% CAPTISOL. In yet another embodiment, the composition comprises at least about 15% CAPTISOL. In a further embodiment, the composition comprises from about 25 0.5 to about 50% CAPTISOL. In yet another embodiment, the composition comprises from about 0.5% to about 40% CAPTISOL. In another embodiment, the composition comprises about 0.5% to about 35% CAPTISOL. In yet another embodiment, the composition comprises about 30% CAPTISOL. In a further embodiment, the composition further comprises one or more acids or 30 bases. In one embodiment, the acid or base is added in an amount of 0.5 to 1.5 mol equivalents, preferably 1 to 1.3 mol equivalents to formulate the compound. Acids that may be included in the composition include inorganic acids such as hydrochloric acid, sulfuric acid and phosphoric acid preferably hydrochloric acid, and organic acids such as citric acid, 51 WO 2008/033749 PCT/US2007/077977 L(-)-malic acid and L(+)-tartaric acid preferably L(+)-tartaric acid. Examples of bases that may be included in the composition include sodium hydroxide and potassium hydroxide preferably sodium hydroxide. In a further embodiment, the composition optionally comprises dextran. In yet 5 another embodiment, the composition comprises dextran in an amount of range from about 1% to about 5% weight/volume dextran. In a further embodiment, the composition comprises from about 2 to about 4 % weight/volume dextran. The composition can be stored prior to administration to a patient. In one embodiment, the composition is stored as a ready-to-use formulation. In yet another 10 embodiment, the composition is stored having a diluted concentration of the active compound. The composition may be diluted with any appropriate excipient, for example, dextran or water. In a further embodiment, the composition is stored having a higher concentration of active compound for later dilution prior to administration. An example of such a solubilizing agent is sold under the trademark CAPTISOL@ by CyDex, Inc. 15 CAPTISOL is a polyanionic B-cyclodextrin derivative with a sodium sulfonate salt separated from the lipophilic cavity by a butyl ether spacer group, or sulfobutylether (SBE) (for example, as described in U.S. Pat. No. 5,134,127, the contents of which are herein incorporated by reference). The selection of the SBE7-B-CD as the cyclodextrin with the most desirable safety profile and drug carrier properties was based upon evaluations of the 20 mono, tetra and hepta-substituted preparations (SBE1, SBE4, and SBE7). CAPTISOL is the trade name for CyDex's SBE7-B-CD PRODUCT. Relative to B-cyclodextrin, the preferred solubilizing agents, such as CAPTISOL@, provide superior water solubility in excess of 70, preferably 90 grams/I00 ml. In one embodiment, the solubilizing agent is added to the aqueous solution in an 25 amount of between about 0.5% and 50%, such as between about 0.5% and 40% or about 0.5% and 35%, preferably between about 5% and 30%, more preferably between about 15 and 30%, such as about 30% weight/volume. Additional optional excipients can include dextran in an amount less than about 10%, such as less than about 5% weight/volume, such as about 5% weight/volume or between about 2 to 4 % weight/volume. 30 Additional acids or bases may be added in an amount between about 0.5 to 1.5 mol equivalents, preferably between about 1 to 1.3 mol equivalents to further facilitate solubilization of the compound. Such acids can include inorganic acids such as hydrochloric acid, sulfuric acid and phosphoric acid preferably hydrochloric acid, and 52 WO 2008/033749 PCT/US2007/077977 organic acids such as citric acid, L(-)-malic acid and L(+)-tartaric acid preferably L(+) tartaric acid; such bases can include sodium hydroxide and potassium hydroxide preferably sodium hydroxide. The pharmaceutical compositions can be included in a container, pack, or dispenser 5 together with instructions for administration. Daily administration may be repeated continuously for a period of several days to several years. Oral treatment may continue for between one week and the life of the patient. Preferably the administration may take place for five consecutive days after which time the patient can be evaluated to determine if further administration is required. The 10 administration can be continuous or intermittent, e.g., treatment for a number of consecutive days followed by a rest period. The compounds of the present invention may be administered intravenously on the first day of treatment, with oral administration on the second day and all consecutive days thereafter. The preparation of pharmaceutical compositions that contain an active component is 15 well understood in the art, for example, by mixing, granulating, or tablet-forming processes. The active therapeutic ingredient is often mixed with excipients that are pharmaceutically acceptable and compatible with the active ingredient. For oral administration, the active agents are mixed with additives customary for this purpose, such as vehicles, stabilizers, or inert diluents, and converted by customary methods into suitable forms for administration, 20 such as tablets, coated tablets, hard or soft gelatin capsules, aqueous, alcoholic or oily solutions and the like as detailed above. The amount of the compound administered to the patient is less than an amount that would cause toxicity in the patient. In certain embodiments, the amount of the compound that is administered to the patient is less than the amount that causes a concentration of the 25 compound in the patient's plasma to equal or exceed the toxic level of the compound. Preferably, the concentration of the compound in the patient's plasma is maintained at about 10 nM. In one embodiment, the concentration of the compound in the patient's plasma is maintained at about 25 nM. In one embodiment, the concentration of the compound in the patient's plasma is maintained at about 50 nM. In one embodiment, the concentration of the 30 compound in the patient's plasma is maintained at about 100 nM. In one embodiment, the concentration of the compound in the patient's plasma is maintained at about 500 nM. In one embodiment, the concentration of the compound in the patient's plasma is maintained at about 1000 nM. In one embodiment, the concentration of the compound in the patient's 53 WO 2008/033749 PCT/US2007/077977 plasma is maintained at about 2500 nM. In one embodiment, the concentration of the compound in the patient's plasma is maintained at about 5000 nM. The optimal amount of the compound that should be administered to the patient in the practice of the present invention will depend on the particular compound used and the type of cancer being treated. 5 DEFINITIONS Listed below are definitions of various terms used to describe this invention. These definitions apply to the terms as they are used throughout this specification and claims, unless otherwise limited in specific instances, either individually or as part of a larger group. 10 An "aliphatic group" or "aliphatic" is non-aromatic moiety that may be saturated (e.g. single bond) or contain one or more units of unsaturation, e.g., double and/or triple bonds. An aliphatic group may be straight chained, branched or cyclic, contain carbon, hydrogen or, optionally, one or more heteroatoms and may be substituted or unsubstituted. An aliphatic group preferably contains between about 1 and about 24 atoms, more 15 preferably between about 4 to about 24 atoms, more preferably between about 4-12 atoms, more typically between about 4 and about 8 atoms. The term "acyl" refers to hydrogen, alkyl, partially saturated or fully saturated cycloalkyl, partially saturated or fully saturated heterocycle, aryl, and heteroaryl substituted carbonyl groups. For example, acyl includes groups such as (Ci-C 6 )atkanoyl (e.g., formyl, 20 acetyl, propionyl, butyryl, valeryl, caproyl, t-butylacetyl, etc.), (C3-C6)cycloalfkylcarbonyl (e.g., cyclopropylcarbonyl, cyclobutylcarbonyl, cyclopentylcarbonyl, cyclohexylcarbonyl, etc.), heterocyclic carbonyl (e.g., pyrrolidinylcarbonyl, pyrrolid-2-one-5-carbonyl, piperidinylcarbonyl, piperazinylcarbonyl, tetrahydiofuranylcarbonyl, etc.), aroyl (e.g., benzoyl) and heteroaroyl (e.g., thiophenyl-2-carbonyl, thiophenyl-3-carbonyl, furanyl-2 25 carbonyl, furanyl-3-carbonyl, 1H-pyrroyl-2-carbonyl, 1H-pyrroyl-3-carbonyl, benzo[b]thiophenyt-2-carbonyl, etc.). In addition, the alkyl, cycloalkyl, heterocycle, aryl and heteroaryl portion of the acyl group may be any one of the groups described in the respective definitions. When indicated as being "optionally substituted", the acyl group may be unsubstituted or optionally substituted with one or more substituents (typically, one to 30 three substituents) independently selected from the group of substituents listed below in the definition for "substituted" or the alkyl, cycloalkyl, heterocycle, aryl and heteroaryl portion of the acyl group may be substituted as described above in the preferred and more preferred list of substituents, respectively. 54 WO 2008/033749 PCT/US2007/077977 The term "alkyl" embraces linear or branched radicals having one to about twenty carbon atoms or, preferably, one to about twelve carbon atoms. More preferred alkyl radicals are "lower alkyl" radicals having one to about ten carbon atoms. Most preferred are lower alkyl radicals having one to about eight carbon atoms. Examples of such radicals 5 include methyl, ethyl, n-propyl, isopropyl, n-butyl, isobutyl, sec-butyl, tert-butyl, pentyl, iso-amyl, hexyl and the like. The term "alkenyl" embraces linear or branched radicals having at least one carbon carbon double bond of two to about twenty carbon atoms or, preferably, two to about twelve carbon atoms. More preferred alkenyl radicals are "lower alkenyl" radicals having two to 10 about ten and more preferably about two to about eight carbon atoms. Examples of alkenyl radicals include ethenyl, allyl, propenyl, butenyl and 4-methylbutenyl. The terms "alkenyl", and "lower alkenyl", embrace radicals having "cis" and "trans" orientations, or alternatively, "E" and "Z" orientations. The term "alkynyl" embraces linear or branched radicals having at least one carbon 15 carbon triple bond of two to about twenty carbon atoms or, preferably, two to about twelve carbon atoms. More preferred alkynyl radicals are "lower alkynyl" radicals having two to about ten and more preferably two to about eight carbon atoms. Examples of alkynyl radicals include propargyl, I -propynyl, 2-propynyl, I-butyne, 2-butynyl and I -pentynyl. The term "cycloalkyl" embraces saturated carbocyclic radicals having three to about 20 twelve carbon atoms. The term "cycloalkyl" embraces saturated carbocyclic radicals having three to about twelve carbon atoms. More preferred cycloalkyl radicals are "lower cycloalkyl" radicals having three to about eight carbon atoms. Examples of such radicals include cyclopropyl, cyclobutyl, cyclopentyl and cyclohexyl. The term "cycloalkenyl" embraces partially unsaturated carbocyclic radicals having 25 three to twelve carbon atoms. Cycloalkenyl radicals that are partially unsaturated carbocyclic radicals that contain two double bonds (that may or may not be conjugated) can be called "cycloalkyldienyl". More preferred cycloalkenyl radicals are "lower cycloalkenyl" radicals having four to about eight carbon atoms. Examples of such radicals include cyclobutenyl, cyclopentenyl and cyclohexenyl. 30 The term "alkoxy" embraces linear or branched oxy-containing radicals each having alkyl portions of one to about twenty carbon atoms or, preferably, one to about twelve carbon atoms. More preferred alkoxy radicals are "lower alkoxy" radicals having one to 55 WO 2008/033749 PCT/US2007/077977 about ten and more preferably having one to about eight carbon atoms. Examples of such radicals include methoxy, ethoxy, propoxy, butoxy and tert-butoxy. The term "alkoxyalkyl" embraces alkyl radicals having one or more alkoxy radicals attached to the alkyl radical, that is, to form monoalkoxyalkyl and dialkoxyalkyl radicals. 5 The term "aryl", alone or in combination, means a carbocyclic aromatic system containing one, two or three rings wherein such rings may be attached together in a pendent manner or may be fused. The term "aryl" embraces aromatic radicals such as phenyl, naphthyl, tetrahydronaphthyl, indane and biphenyl. The term "carbonyt", whether used alone or with other terms, such as 10 "alkoxycarbonyl", denotes (C=0). The term "carbanoyl", whether used alone or with other terms, such as "arylcarbanoylyalkyl", denotes C(O)NH. The terms "heterocyclyl", "heterocycle" "heterocyclic" or "heterocyclo" embrace saturated, partially unsaturated and unsaturated heteroatom-containing ring-shaped radicals, 15 which can also be called "heterocyclyl", "heterocycloalkenyl" and "heteroaryl" correspondingly, where the heteroatoms may be selected from nitrogen, sulfur and oxygen. Examples of saturated heterocyclyl radicals include saturated 3 to 6-membered heteromonocyclic group containing I to 4 nitrogen atoms (e.g. pyrrolidinyl, imidazolidinyl, piperidino, piperazinyl, etc.); saturated 3 to 6-membered heteromonocyclic group 20 containing I to 2 oxygen atoms and 1 to 3 nitrogen atoms (e.g. morpholinyl, etc.); saturated 3 to 6-membered heteromonocyclic group containing 1 to 2 sulfur atoms and 1 to 3 nitrogen atoms (e.g., thiazolidinyl, etc.). Examples of partially unsaturated heterocyclyl radicals include dihydrothiophene, dihydropyran, dihydrofuran and dihydrothiazole. Heterocyclyl radicals may include a pentavalent nitrogen, such as in tetrazolium and pyridinium radicals. 25 The term "heterocycle" also embraces radicals where heterocyclyl radicals are fused with aryl or cycloalkyl radicals. Examples of such fused bicyclic radicals include benzofuran, benzothiophene, and the like. The term "heteroaryl" embraces unsaturated heterocyclyl radicals. Examples of heteroaryl radicals include unsaturated 3 to 6 membered heteromonocyclic group containing 30 1 to 4 nitrogen atoms, for example, pyrrolyl, pyrrolinyl, imidazolyl, pyrazolyl, pyridyl, pyrimidyl, pyrazinyl, pyridazinyl, triazolyl (e.g., 4H-1,2,4-triazolyl, 1H-1,2,3-triazolyl, 2H 1,2,3-triazolyl, etc.) tetrazolyl (e.g. 1H-tetrazolyl, 2H-tetrazolyl, etc.), etc.; unsaturated condensed heterocyclyl group containing 1 to 5 nitrogen atoms, for example, indolyl, 56 WO 2008/033749 PCT/US2007/077977 isoindolyl, indolizinyl, benzimidazolyl, quinolyl, isoquinolyl, indazolyl, benzotriazolyl, tetrazolopyridazinyl (e.g., tetrazolo[1,5-b]pyridazinyl, etc.), etc.; unsaturated 3 to 6 membered heteromonocyclic group containing an oxygen atom, for example, pyranyl, furyl, etc.; unsaturated 3 to 6-membered heteromonocyclic group containing a sulfur atom, for 5 example, thienyl, etc.; unsaturated 3- to 6-membered heteromonocyclic group containing 1 to 2 oxygen atoms and 1 to 3 nitrogen atoms, for example, oxazolyl, isoxazolyl, oxadiazolyl (e.g., 1,2,4-oxadiazolyl, 1,3,4-oxadiazolyl, 1,2,5-oxadiazolyl, etc.) etc.; unsaturated condensed heterocyclyl group containing 1 to 2 oxygen atoms and I to 3 nitrogen atoms (e.g. benzoxazolyl, benzoxadiazolyl, etc.); unsaturated 3 to 6-membered heteromonocyclic 10 group containing 1 to 2 sulfur atoms and 1 to 3 nitrogen atoms, for example, thiazolyl, thiadiazolyl (e.g., 1,2,4- thiadiazolyl, 1,3,4-thiadiazolyl, 1,2,5-thiadiazolyl, etc.) etc.; unsaturated condensed heterocyclyl group containing 1 to 2 sulfur atoms and 1 to 3 nitrogen atoms (e.g., benzothiazolyl, benzothiadiazolyl, etc.) and the like. The term "heterocycloalkyl" embraces heterocyclo-substituted alkyl radicals. More 15 preferred heterocycloalkyl radicals are "lower heterocycloalkyl" radicals having one to six carbon atoms in the heterocyclo radicals. The term "alkylthio" embraces radicals containing a linear or branched alkyl radical of one to about ten carbon atoms attached to a divalent sulfur atom. Preferred alkylthio radicals have alkyl radicals of one to about twenty carbon atoms or, preferably, one to about 20 twelve carbon atoms. More preferred alkylthio radicals have alkyl radicals are "lower alkylthio" radicals having one to about ten carbon atoms. Most preferred are alkylthio radicals having lower alkyl radicals of one to about eight carbon atoms. Examples of such lower alkylthio radicals are methylthio, ethylthio, propylthio, butylthio and hexylthio. The terms "aralkyl" or "arylalkyl" embrace aryl-substituted alkyl radicals such as 25 benzyl, diphenylmethyl, triphenylmethyl, phenylethyl, and diphenylethyl. The term "aryloxy" embraces aryl radicals attached through an oxygen atom to other radicals. The terms "araikoxy" or "arylalkoxy" embrace aralkyl radicals attached through an oxygen atom to other radicals. 30 The term "aminoalkyl" embraces alkyl radicals substituted with amino radicals. Preferred aminoalkyl radicals have alkyl radicals having about one to about twenty carbon atoms or, preferably, one to about twelve carbon atoms. More preferred aminoalkyl radicals are "lower aminoalkyl" that have alkyl radicals having one to about ten carbon atoms. Most 57 WO 2008/033749 PCT/US2007/077977 preferred are aminoalkyl radicals having lower alkyl radicals having one to eight carbon atoms. Examples of such radicals include aminomethyl, aminoethyl, and the like. The term "alkylamino" denotes amino groups which are substituted with one or two alkyl radicals. Preferred alkylamino radicals have alkyl radicals having about one to about 5 twenty carbon atoms or, preferably, one to about twelve carbon atoms. More preferred alkylamino radicals are "lower alkylamino" that have alkyl radicals having one to about ten carbon atoms. Most preferred are alkylamino radicals having lower alkyl radicals having one to about eight carbon atoms. Suitable lower alkylamino may be monosubstituted N alkylamino or disubstituted N,N-alkylamino, such as N-methylamino, N-ethylamino, N,N 10 dimethylamino, N,N-diethylamino or the like. The term "linker" means an organic moiety that connects two parts of a compound. Linkers typically comprise a direct bond or an atom such as oxygen or sulfur, a unit such as NRs, C(O), C(O)NH, SO, S02, SO 2 NH or a chain of atoms, such as substituted or unsubstituted alkyl, substituted or unsubstituted alkenyl, substituted or unsubstituted 15 alkynyl, arylalkyl, arylalkenyl, arylalkynyl, heteroarylalkyl, heteroarylalkenyl, heteroarylalkynyl, heterocyclylalkyl, heterocyclylalkenyl, heterocyclylalkynyl, aryl, heteroaryl, heterocyclyl, cycloalkyl, cycloalkenyl, alkylarylalkyl, alkylarylalkenyl, alkylarylalkynyl, alkenylarylalkyl, alkenylarylalkenyl, alkenylarylalkynyl, alkynylarylalkyl, alkynylarylalkenyl, alkynylarylalkynyl, alkylheteroarylalkyl, alkylheteroarylalkenyl, 20 alkylheteroarylalkynyl, alkenylheteroarylalkyl, alkenylheteroarylalkenyl, alkenylheteroarylalkynyl, alkynylheteroarylalkyl, alkynylheteroarylalkenyl, alkynylheteroarylalkynyl, alkylheterocyclylalkyl, alkylheterocyclylalkenyl, alkylhererocyclylalkynyl, alkenylheterocyclylalkyl, alkenylheterocyclylalkenyl, alkenylheterocyclylalkynyl, alkynylheterocyclylalkyl, alkynylheterocyclylalkenyl, 25 alkynylheterocyclylalkynyl, alkylaryl, alkenylaryl, alkynylaryl, alkylheteroaryl, alkenylheteroaryl, alkynylhereroaryl, which one or more methylenes can be interrupted or terminated by 0, S, S(O), S02, N(R 8 ), C(O), substituted or unsubstituted aryl, substituted or unsubstituted heteroaryl, substituted or unsubstituted heterocyclic; where R 8 is hydrogen, acyl, aliphatic or substituted aliphatic. In one embodiment, the linker B is between 1-24 30 atoms, preferably 4-24 atoms, preferably 4-18 atoms, more preferably 4-12 atoms, and most preferably about 4-10 atoms. The term "substituted" refers to the replacement of one or more hydrogen radicals in a given structure with the radical of a specified substituent including, but not limited to: 58 WO 2008/033749 PCT/US2007/077977 halo, alkyl, alkenyl, alkynyl, aryl, heterocyclyl, thiol, atkylthio, arylthio, alkylthioalkyl, arylthioalkyl, alkylsulfonyl, alkylsulfonylalkyl, arylsulfonylalkyl, alkoxy, aryloxy, aralkoxy, aminocarbonyl, alkylaminocarbonyl, arylaminocarbonyl, alkoxycarbonyl, aryloxycarbonyl, haloalkyl, amino, trifluoromethyl, cyano, nitro, alkylamino, arylamino, 5 alkylaminoalkyl, arylaminoalkyl, aminoalkylamino, hydroxy, alkoxyalkyl, carboxyalkyl, alkoxycarbonylalkyl, aminocarbonylalkyl, acyl, aralkoxycarbonyl, carboxylic acid, sulfonic acid, sulfonyl, phosphonic acid, aryl, heteroaryl, heterocyclic, and aliphatic. It is understood that the substituent can be further substituted. For simplicity, chemical moieties are defined and referred to throughout can be 10 univalent chemical moieties (e.g., alkyl, aryl, etc.) or multivalent moieties under the appropriate structural circumstances clear to those skilled in the art. For example, an "alkyl" moiety can be referred to a monovalent radical (e.g. CH 3

-CH

2 -), or in other instances, a bivalent linking moiety can be "alkyl," in which case those skilled in the art will understand the alkyl to be a divalent radical (e.g., -CH-CH 2 ), which is equivalent to the term 15 "alkylene." Similarly, in circumstances in which divalent moieties are required and are stated as being "alkoxy", "alkylamino", "aryloxy", "alkylthio", "aryl", "heteroaryl", "heterocyclic", "alkyl" "alkenyl", "alkynyl", "aliphatic", or "cycloalkyl", those skilled in the art will understand that the terms alkoxy", "alkylamino", "aryloxy", "alkylthio", "aryl", "heteroaryl", "heterocyclic", "alkyl", "alkenyl", "alkynyl", "aliphatic", or "cycloalkyl" 20 refer to the corresponding divalent moiety. The terms "halogen" or "halo" as used herein, refers to an atom selected from fluorine, chlorine, bromine and iodine. As used herein, the term "aberrant proliferation" refers to abnormal cell growth. The phrase "adjunctive therapy" encompasses treatment of a subject with agents that 25 reduce or avoid side effects associated with the combination therapy of the present invention, including, but not limited to, those agents, for example, that reduce the toxic effect of anticancer drugs, e.g., bone resorption inhibitors, cardioprotective agents; prevent or reduce the incidence of nausea and vomiting associated with chemotherapy, radiotherapy or operation; or reduce the incidence of infection associated with the administration of 30 myelosuppressive anticancer drugs. The term "angiogenesis," as used herein, refers to the formation of blood vessels. Specifically, angiogenesis is a multi-step process in which endothelial cells focally degrade and invade through their own basement membrane, migrate through interstitial stroma 59 WO 2008/033749 PCT/US2007/077977 toward an angiogenic stimulus, proliferate proximal to the migrating tip, organize into blood vessels, and reattach to newly synthesized basement membrane (see Folkman et al., Adv. Cancer Res., Vol. 43, pp. 175-203 (1985)). Anti-angiogenic agents interfere with this process. Examples of agents that interfere with several of these steps include 5 thrombospondin-1, angiostatin, endostatin, interferon alpha and compounds such as matrix metalloproteinase (MMP) inhibitors that block the actions of enzymes that clear and create paths for newly forming blood vessels to follow; compounds, such as .alpha.v.beta.3 inhibitors, that interfere with molecules that blood vessel cells use to bridge between a parent blood vessel and a tumor; agents, such as specific COX-2 inhibitors, that prevent the 10 growth of cells that form new blood vessels; and protein-based compounds that simultaneously interfere with several of these targets. The term "apoptosis" as used herein refers to programmed cell death as signaled by the nuclei in normally functioning human and animal cells when age or state of cell health and condition dictates. An "apoptosis inducing agent" triggers the process of programmed 15 cell death. The term "cancer" as used herein denotes a class of diseases or disorders characterized by uncontrolled division of cells and the ability of these cells to invade other tissues, either by direct growth into adjacent tissue through invasion or by implantation into distant sites by metastasis. 20 The term "compound" is defined herein to include pharmaceutically acceptable salts, solvates, hydrates, polymorphs, enantiomers, diastereoisomers, racemates and the like of the compounds having a formula as set forth herein. The phrase a "devices" refers to any appliance, usually mechanical or electrical, designed to perform a particular function. 25 As used herein, the term "dysplasia" refers to abnormal cell growth and typically refers to the earliest form of pre-cancerous lesion recognizable in a biopsy by a pathologist. As used herein, the term "effective amount of the subject compounds," with respect to the subject method of treatment, refers to an amount of the subject compound which, when delivered as part of desired dose regimen, brings about, e.g. a change in the rate of 30 cell proliferation and/or state of differentiation and/or rate of survival of a cell to clinically acceptable standards. This amount may further relieve to some extent one or more of the symptoms of a neoplasia disorder, including, but is not limited to: 1) reduction in the number of cancer cells; 2) reduction in tumor size; 3) inhibition (i.e., slowing to some 60 WO 2008/033749 PCT/US2007/077977 extent, preferably stopping) of cancer cell infiltration into peripheral organs; 4) inhibition (i.e., slowing to some extent, preferably stopping) of tumor metastasis; 5) inhibition, to some extent, of tumor growth; 6) relieving or reducing to some extent one or more of the symptoms associated with the disorder; and/or 7) relieving or reducing the side effects 5 associated with the administration of anticancer agents. The term "hyperplasia," as used herein, refers to excessive cell division or growth. The phrase an "immunotherapeutic agent" refers to agents used to transfer the immunity of an immune donor, e.g., another person or an animal, to a host by inoculation. The term embraces the use of serum or gamma globulin containing performed antibodies 10 produced by another individual or an animal; nonspecific systemic stimulation; adjuvants; active specific immunotherapy; and adoptive immunotherapy. Adoptive immunotherapy refers to the treatment of a disease by therapy or agents that include host inoculation of sensitized lymphocytes, transfer factor, immune RNA, or antibodies in serum or gamma globulin. 15 The term "inhibition," in the context of neoplasia, tumor growth or tumor cell growth, may be assessed by delayed appearance of primary or secondary tumors, slowed development of primary or secondary tumors, decreased occurrence of primary or secondary tumors, slowed or decreased severity of secondary effects of disease, arrested tumor growth and regression of tumors, among others. In the extreme, complete inhibition, 20 is referred to herein as prevention or chemoprevention. The term "metastasis," as used herein, refers to the migration of cancer cells from the original tumor site through the blood and lymph vessels to produce cancers in other tissues. Metastasis also is the term used for a secondary cancer growing at a distant site. The term "neoplasm," as used herein, refers to an abnormal mass of tissue that 25 results from excessive cell division. Neoplasms may be benign (not cancerous), or malignant (cancerous) and may also be called a tumor. The term "neoplasia" is the pathological process that results in tumor formation. As used herein, the term "pre-cancerous" refers to a condition that is not malignant, but is likely to become malignant if left untreated. 30 The term "proliferation" refers to cells undergoing mitosis. The phrase "EGFR-TK related disease or disorder" refers to a disease or disorder characterized by inappropriate EGFR-TK activity or over-activity of the EGFR-TK. Inappropriate activity refers to either; (i) EGFR-TK expression in cells which normally do 61 WO 2008/033749 PCT/US2007/077977 not express EGFR-TKs; (ii) increased EGFR-TK expression leading to unwanted cell proliferation, differentiation and/or growth; or, (iii) decreased EGFR-TK expression leading to unwanted reductions in cell proliferation, differentiation and/or growth. Over-activity of EGFR-TKs refers to either amplification of the gene encoding a particular EGFR-TK or 5 production of a level of EGFR-TK activity which can correlate with a cell proliferation, differentiation and/or growth disorder (that is, as the level of the EGFR-TK increases, the severity of one or more of the symptoms of the cellular disorder increases). Over activity can also be the result of ligand independent or constitutive activation as a result of mutations such as deletions of a fragment of a EGFR-TK responsible for ligand binding. 10 The phrase a "radio therapeutic agent" refers to the use of electromagnetic or particulate radiation in the treatment of neoplasia. The term "recurrence" as used herein refers to the return of cancer after a period of remission. This may be due to incomplete removal of cells from the initial cancer and may occur locally (the same site of initial cancer), regionally (in vicinity of initial cancer, 15 possibly in the lymph nodes or tissue), and/or distally as a result of metastasis. The term "treatment" refers to any process, action, application, therapy, or the like, wherein a mammal, including a human being, is subject to medical aid with the object of improving the mammal's condition, directly or indirectly. The term "vaccine" includes agents that induce the patient's immune system to 20 mount an immune response against the tumor by attacking cells that express tumor associated antigens (Teas). As used herein, the term "pharmaceutically acceptable salt" refers to those salts which are, within the scope of sound medical judgment, suitable for use in contact with the tissues of humans and lower animals without undue toxicity, irritation, allergic response and 25 the like, and are commensurate with a reasonable benefit/risk ratio. Pharmaceutically acceptable salts are well known in the art. For example, S. M. Berge, et al. describes pharmaceutically acceptable salts in detail in J. Pharmaceutical Sciences, 66: 1-19 (1977). The salts can be prepared in situ during the final isolation and purification of the compounds of the invention, or separately by reacting the free base function with a suitable 30 organic acid or inorganic acid. Examples of pharmaceutically acceptable nontoxic acid addition salts include, but are not limited to, salts of an amino group formed with inorganic acids such as hydrochloric acid, hydrobromic acid, phosphoric acid, sulfuric acid and perchloric acid or with organic acids such as acetic acid, maleic acid, tartaric acid, citric 62 WO 2008/033749 PCT/US2007/077977 acid, succinic acid lactobionic acid or malonic acid or by using other methods used in the art such as ion exchange. Other pharmaceutically acceptable salts include, but are not limited to, adipate, alginate, ascorbate, aspartate, benzenesulfonate, benzoate, bisulfate, borate, butyrate, camphorate, camphorsulfonate, citrate, cyclopentanepropionate, 5 digluconate, dodecylsulfate, ethanesulfonate, formate, fumarate, glucoheptonate, glycerophosphate, gluconate, hemisulfate, heptanoate, hexanoate, hydroiodide, 2-hydroxy ethanesulfonate, lactobionate, lactate, laurate, lauryl sulfate, malate, maleate, malonate, methanesulfonate, 2-naphthalenesulfonate, nicotinate, nitrate, oleate, oxalate, palmitate, pamoate, pectinate, persulfate, 3-phenylpropionate, phosphate, picrate, pivalate, propionate, 10 stearate, succinate, sulfate, tartrate, thiocyanate, p-toluenesulfonate, undecanoate, valerate salts, and the like. Representative alkali or alkaline earth metal salts include sodium, lithium, potassium, calcium, magnesium, and the like. Further pharmaceutically acceptable salts include, when appropriate, nontoxic ammonium, quaternary ammonium, and amine cations formed using counterions such as halide, hydroxide, carboxylate, sulfate, phosphate, 15 nitrate, alkyl having from 1 to 6 carbon atoms, sulfonate and aryl sulfonate. As used herein, the term "pharmaceutically acceptable ester" refers to esters which hydrolyze in vivo and include those that break down readily in the human body to leave the parent compound or a salt thereof. Suitable ester groups include, for example, those derived from pharmaceutically acceptable aliphatic carboxylic acids, particularly alkanoic, alkenoic, 20 cycloalkanoic and alkanedioic acids, in which each alkyl or alkenyl moiety advantageously has not more than 6 carbon atoms. Examples of particular esters include, but are not limited to, formates, acetates, propionates, butyrates, acrylates and ethylsuccinates. The term "pharmaceutically acceptable prodrugs" as used herein refers to those prodrugs of the compounds of the present invention which are, within the scope of sound 25 medical judgment, suitable for use in contact with the tissues of humans and lower animals with undue toxicity, irritation, allergic response, and the like, commensurate with a reasonable benefit/risk ratio, and effective for their intended use, as well as the zwitterionic forms, where possible, of the compounds of the present invention. "Prodrug", as used herein means a compound which is convertible in vivo by metabolic means (e.g. by 30 hydrolysis) to a compound of the invention. Various forms of prodrugs are known in the art, for example, as discussed in Bundgaard, (ed.), Design of Prodrugs, Elsevier (1985); Widder, et al. (ed.), Methods in Enzymology, vol. 4, Academic Press (1985); Krogsgaard Larsen, et al., (ed). "Design and Application of Prodrugs, Textbook of Drug Design and 63 WO 2008/033749 PCT/US2007/077977 Development, Chapter 5, 113-191 (1991); Bundgaard, et al., Journal of Drug Deliver Reviews, 8:1-38(1992); Bundgaard, J. of Pharmaceutical Sciences, 77:285 et seq. (1988); Higuchi and Stella (eds.) Prodrugs as Novel Drug Delivery Systems, American Chemical Society (1975); and Bernard Testa & Joachim Mayer, "Hydrolysis In Drug And Prodrug 5 Metabolism: Chemistry, Biochemistry And Enzymology," John Wiley and Sons, Ltd. (2002). As used herein, pharmaceuticallyy acceptable carrier" is intended to include any and all solvents, dispersion media, coatings, antibacterial and antifungal agents, isotonic and absorption delaying agents, and the like, compatible with pharmaceutical administration, 10 such as sterile pyrogen-free water. Suitable carriers are described in the most recent edition of Remington's Pharmaceutical Sciences, a standard reference text in the field, which is incorporated herein by reference. Preferred examples of such carriers or diluents include, but are not limited to, water, saline, finger's solutions, dextrose solution, and 5% human serum albumin. Liposomes and non-aqueous vehicles such as fixed oils may also be used. 15 The use of such media and agents for pharmaceutically active substances is well known in the art. Except insofar as any conventional media or agent is incompatible with the active compound, use thereof in the compositions is contemplated. Supplementary active compounds can also be incorporated into the compositions. As used herein, the term "pre-cancerous" refers to a condition that is not malignant, 20 but is likely to become malignant if left untreated. The term "subject" as used herein refers to an animal. Preferably the animal is a mammal. More preferably the mammal is a human. A subject also refers to, for example, dogs, cats, horses, cows, pigs, guinea pigs, fish, birds and the like. The compounds of this invention may be modified by appending appropriate 25 functionalities to enhance selective biological properties. Such modifications are known in the art and may include those which increase biological penetration into a given biological system (e.g., blood, lymphatic system, central nervous system), increase oral availability, increase solubility to allow administration by injection, alter metabolism and alter rate of excretion. 30 The synthesized compounds can be separated from a reaction mixture and further purified by a method such as column chromatography, high pressure liquid chromatography, or recrystallization. As can be appreciated by the skilled artisan, further methods of synthesizing the compounds of the formulae herein will be evident to those of 64 WO 20081033749 PCT/US2007/077977 ordinary skill in the art. Additionally, the various synthetic steps may be performed in an alternate sequence or order to give the desired compounds. Synthetic chemistry transformations and protecting group methodologies (protection and deprotection) useful in synthesizing the compounds described herein are known in the art and include, for example, 5 those such as described in R. Larock, Comprehensive Organic Transformations, VCH Publishers (1989); T.W. Greene and P.G.M. Wuts, Protective Groups in Organic Synthesis, 2d. Ed., John Wiley and Sons (1991); L. Fieser and M. Fieser, Fieser and Fieser's Reagents for Organic Synthesis, John Wiley and Sons (1994); and L. Paquette, ed., Encyclopedia of Reagents for Organic Synthesis, John Wiley and Sons (1995), and subsequent editions 10 thereof. The compounds described herein contain one or more asymmetric centers and thus give rise to enantiomers, diastereomers, and other stereoisomeric forms that may be defined, in terms of absolute stereochemistry, as (R)- or (S)- , or as (D)- or (L)- for amino acids. The present invention is meant to include all such possible isomers, as well as their racemic and 15 optically pure forms. Optical isomers may be prepared from their respective optically active precursors by the procedures described above, or by resolving the racemic mixtures. The resolution can be carried out in the presence of a resolving agent, by chromatography or by repeated crystallization or by some combination of these techniques which are known to those skilled in the art. Further details regarding resolutions can be found in Jacques, et al., 20 Enantiomers, Racemates, and Resolutions (John Wiley & Sons, 1981). When the compounds described herein contain olefinic double bonds, other unsaturation, or other centers of geometric asymmetry, and unless specified otherwise, it is intended that the compounds include both E and Z geometric isomers and/or cis- and trans- isomers. Likewise, all tautomeric forms are also intended to be included. The configuration of any 25 carbon-carbon double bond appearing herein is selected for convenience only and is not intended to designate a particular configuration unless the text so states; thus a carbon carbon double bond or carbon-heteroatom double bond depicted arbitrarily herein as trans may be cis, trans, or a mixture of the two in any proportion. In some embodiments, the inventive compound is a crystalline or an amorphous 30 form of a compound of the invention. In another embodiment, the inventive compound is a mixture of two or more crystalline forms of the compound. In a further embodiment, the invention is a crystalline form or a mixture of two or more crystalline forms of compound 65 WO 20081033749 PCT/US20071077977 12. A crystalline form of compound 12 can be prepared substantially as described in Example 8 (Method 2) below. Different crystalline forms of chemical compounds (or polymorphs) each exhibit different physical, chemical, spectroscopic and/or crystallographic properties. In one 5 embodiment, the crystalline form of the invention has an X-ray powder diffraction (XRPD) pattern with at least three, four, five, six, seven or eight major peaks in common with the X ray powder diffraction pattern of the crystal prepared according to working Example 8 (Method 2). A major peak is an XRPD peak with a relative intensity greater than about 25%; relative intensity is calculated as a ratio of the peak intensity of the peak of interest 10 versus the peak intensity of the largest peak. As used herein, a peak for the crystalline form is in common with the XRPD pattern of the crystal prepared according to Example 8 (Method 2) if it is within 0.5' 20 of a peak location for the crystal prepared according to Example 8 (Method 2). In another embodiment, the crystalline form of compound 12 has an endothermic transition within about 1.0 0 C as the crystalline form prepared according to 15 Example 8 (Method 2). In yet another embodiment, the crystalline form of compound 12 has an endothermic transition (as observed by differential scanning calorimetry) within about 0.5'C of as the crystal formed according to Example 8 (Method 2) and at least three, four, five, six, seven or eight major peaks in common with the XRPD pattern of the compound prepared according to Example 8 (Method 2). In a further embodiment, the 20 crystalline form of compound 12 exhibits a differential scanning calorimetry pattern that is substantially the same as the differential scanning calorimetry pattern of the compound produced according the Example 8 (Method 2). Pharmaceutical Compositions 25 The pharmaceutical compositions of the present invention comprise a therapeutically effective amount of a compound of the present invention formulated together with one or more pharmaceutically acceptable carriers or excipients. As used herein, the term "pharmaceutically acceptable carrier or excipient" means a non-toxic, inert solid, semi-solid or liquid filler, diluent, encapsulating material or 30 formulation auxiliary of any type. Some examples of materials which can serve as pharmaceutically acceptable carriers are sugars such as lactose, glucose and sucrose; starches such as corn starch and potato starch; cellulose and its derivatives such as sodium carboxymethyl cellulose, ethyl cellulose and cellulose acetate; powdered tragacanth; malt; 66 WO 20081033749 PCT/US2007/077977 gelatin; talc; excipients such as cocoa butter and suppository waxes; oils such as peanut oil, cottonseed oil, safflower oil, sesame oil, olive oil, corn oil and soybean oil; glycols such as propylene glycol; esters such as ethyl oleate and ethyl laurate; agar; buffering agents such as magnesium hydroxide and aluminum hydroxide; alginic acid; pyrogen-free water; isotonic 5 saline; Ringer's solution; ethyl alcohol, and phosphate buffer solutions, as well as other non toxic compatible lubricants such as sodium lauryl sulfate and magnesium stearate, as well as coloring agents, releasing agents, coating agents, sweetening, flavoring and perfuming agents, preservatives and antioxidants can also be present in the composition, according to the judgment of the formulator. 10 The pharmaceutical compositions of this invention may be administered orally, parenterally, by inhalation spray, topically, rectally, nasally, buccally, vaginally or via an implanted reservoir, preferably by oral administration or administration by injection. The pharmaceutical compositions of this invention may contain any conventional non-toxic pharmaceutically-acceptable carriers, adjuvants or vehicles. In some cases, the pH of the 15 formulation may be adjusted with pharmaceutically acceptable acids, bases or buffers to enhance the stability of the formulated compound or its delivery form. The term parenteral as used herein includes subcutaneous, intracutaneous, intravenous, intramuscular, intraarticular, intraarterial, intrasynovial, intrasternal, intrathecal, intralesional and intracranial injection or infusion techniques. 20 Liquid dosage forms for oral administration include pharmaceutically acceptable emulsions, microemulsions, solutions, suspensions, syrups and elixirs. In addition to the active compounds, the liquid dosage forms may contain inert diluents commonly used in the art such as, for example, water or other solvents, solubilizing agents and emulsifiers such as ethyl alcohol, isopropyl alcohol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl 25 benzoate, propylene glycol, 1,3-butylene glycol, dimethylformamide, oils (in particular, cottonseed, groundnut, corn, germ, olive, castor, and sesame oils), glycerol, tetrahydrofurfuryl alcohol, polyethylene glycols and fatty acid esters of sorbitan, and mixtures thereof. Besides inert diluents, the oral compositions can also include adjuvants such as wetting agents, emulsifying and suspending agents, sweetening, flavoring, and 30 perfuming agents. Injectable preparations, for example, sterile injectable aqueous or oleaginous suspensions, may be formulated according to the known art using suitable dispersing or wetting agents and suspending agents. The sterile injectable preparation may also be a 67 WO 2008/033749 PCT/US2007/077977 sterile injectable solution, suspension or emulsion in a nontoxic parenterally acceptable diluent or solvent, for example, as a solution in 1,3-butanediol. Among the acceptable vehicles and solvents that may be employed are water, Ringer's solution, U.S.P. and isotonic sodium chloride solution. In addition, sterile, fixed oils are conventionally 5 employed as a solvent or suspending medium. For this purpose any bland fixed oil can be employed including synthetic mono- or diglycerides. In addition, fatty acids such as oleic acid are used in the preparation of injectables. The injectable formulations can be sterilized, for example, by filtration through a bacterial-retaining filter, or by incorporating sterilizing agents in the forn of sterile solid 10 compositions which can be dissolved or dispersed in sterile water or other sterile injectable medium prior to use. In order to prolong the effect of a drug, it is often desirable to slow the absorption of the drug from subcutaneous or intramuscular injection. This may be accomplished by the use of a liquid suspension of crystalline or amorphous material with poor water solubility. 15 The rate of absorption of the drug then depends upon its rate of dissolution, which, in turn, may depend upon crystal size and crystalline form. Alternatively, delayed absorption of a parenterally administered drug form is accomplished by dissolving or suspending the drug in an oil vehicle. Injectable depot forms are made by forming microencapsule matrices of the drug in biodegradable polymers such as polylactide-polyglycolide. Depending upon the 20 ratio of drug to polymer and the nature of the particular polymer employed, the rate of drug release can be controlled. Examples of other biodegradable polymers include poly(orthoesters) and poly(anhydrides). Depot injectable formulations are also prepared by entrapping the drug in liposomes or microemulsions that are compatible with body tissues. Compositions for rectal or vaginal administration are preferably suppositories which 25 can be prepared by mixing the compounds of this invention with suitable non-irritating excipients or carriers such as cocoa butter, polyethylene glycol or a suppository wax which are solid at ambient temperature but liquid at body temperature and therefore melt in the rectum or vaginal cavity and release the active compound. Solid dosage forms for oral administration include capsules, tablets, pills, powders, 30 and granules. In such solid dosage forms, the active compound is mixed with at least one inert, pharmaceutically acceptable excipient or carrier such as sodium citrate or dicalcium phosphate and/or: a) fillers or extenders such as starches, lactose, sucrose, glucose, mannitol, and silicic acid, b) binders such as, for example, carboxymethylcellulose, 68 WO 20081033749 PCT/US2007/077977 alginates, gelatin, polyvinylpyrrolidinone, sucrose, and acacia, c) humectants such as glycerol, d) disintegrating agents such as agar-agar, calcium carbonate, potato or tapioca starch, alginic acid, certain silicates, and sodium carbonate, e) solution retarding agents such as paraffin, f) absorption accelerators such as quaternary ammonium compounds, g) wetting 5 agents such as, for example, cetyl alcohol and glycerol monostearate, h) absorbents such as kaolin and bentonite clay, and i) lubricants such as talc, calcium stearate, magnesium stearate, solid polyethylene glycols, sodium lauryl sulfate, and mixtures thereof. In the case of capsules, tablets and pills, the dosage form may also comprise buffering agents. Solid compositions of a similar type may also be employed as fillers in soft and 10 hard-filled gelatin capsules using such excipients as lactose or milk sugar as well as high molecular weight polyethylene glycols and the like. The solid dosage forms of tablets, dragees, capsules, pills, and granules can be prepared with coatings and shells such as enteric coatings and other coatings well known in the pharmaceutical formulating art. They may optionally contain opacifying agents and can 15 also be of a composition that they release the active ingredient(s) only, or preferentially, in a certain part of the intestinal tract, optionally, in a delayed manner. Examples of embedding compositions that can be used include polymeric substances and waxes. Dosage forms for topical or transdermal administration of a compound of this invention include ointments, pastes, creams, lotions, gels, powders, solutions, sprays, 20 inhalants or patches. The active component is admixed under sterile conditions with a pharmaceutically acceptable carrier and any needed preservatives or buffers as may be required. Ophthalmic formulation, ear drops, eye ointments, powders and solutions are also contemplated as being within the scope of this invention. The ointments, pastes, creams and gels may contain, in addition to an active 25 compound of this invention, excipients such as animal and vegetable fats, oils, waxes, paraffins, starch, tragacanth, cellulose derivatives, polyethylene glycols, silicones, bentonites, silicic acid, talc and zinc oxide, or mixtures thereof. Powders and sprays can contain, in addition to the compounds of this invention, excipients such as lactose, talc, silicic acid, aluminum hydroxide, calcium silicates and 30 polyamide powder, or mixtures of these substances. Sprays can additionally contain customary propellants such as chlorofluorohydrocarbons. Transdermal patches have the added advantage of providing controlled delivery of a compound to the body. Such dosage forms can be made by dissolving or dispensing the 69 WO 2008/033749 PCT/US2007/077977 compound in the proper medium. Absorption enhancers can also be used to increase the flux of the compound across the skin. The rate can be controlled by either providing a rate controlling membrane or by dispersing the compound in a polymer matrix or gel. For pulmonary delivery, a therapeutic composition of the invention is formulated 5 and administered to the patient in solid or liquid particulate form by direct administration e.g., inhalation into the respiratory system. Solid or liquid particulate forms of the active compound prepared for practicing the present invention include particles of respirable size: that is, particles of a size sufficiently small to pass through the mouth and larynx upon inhalation and into the bronchi and alveoli of the lungs. Delivery of aerosolized 10 therapeutics, particularly aerosolized antibiotics, is known in the art (see, for example U.S. Pat. No. 5,767,068 to VanDevanter et al., U.S. Pat. No. 5,508,269 to Smith et al., and WO 98/43,650 by Montgomery, all of which are incorporated herein by reference). A discussion of pulmonary delivery of antibiotics is also found in U.S. Pat. No. 6,014,969, incorporated herein by reference. 15 By a "therapeutically effective amount" of a compound of the invention is meant an amount of the compound which confers a therapeutic effect on the treated subject, at a reasonable benefit/risk ratio applicable to any medical treatment. The therapeutic effect may be objective (i.e., measurable by some test or marker) or subjective (i.e., subject gives an indication of or feels an effect). An effective amount of the compound described above 20 may range from about 0.1 mg/Kg to about 500 mg/Kg, preferably from about 1 to about 50 mg/Kg. Effective doses will also vary depending on route of administration, as well as the possibility of co-usage with other agents. It will be understood, however, that the total daily usage of the compounds and compositions of the present invention will be decided by the attending physician within the scope of sound medical judgment. The specific 25 therapeutically effective dose level for any particular patient will depend upon a variety of factors including the disorder being treated and the severity of the disorder; the activity of the specific compound employed; the specific composition employed; the age, body weight, general health, sex and diet of the patient; the time of administration, route of administration, and rate of excretion of the specific compound employed; the duration of the 30 treatment; drugs used in combination or contemporaneously with the specific compound employed; and like factors well known in the medical arts. The total daily dose of the compounds of this invention administered to a human or other animal in single or in divided doses can be in amounts, for example, from 0.0 1 to 50 70 WO 20081033749 PCT/US2007/077977 mg/kg body weight or more usually from 0.1 to 25 mg/kg body weight. Single dose compositions may contain such amounts or submultiples thereof to make up the daily dose. In general, treatment regimens according to the present invention comprise administration to a patient in need of such treatment from about 10 mg to about 1000 mg of the 5 compound(s) of this invention per day in single or multiple doses. The compounds of the formulae described herein can, for example, be administered by injection, intravenously, intraarterially, subdermally, intraperitoneally, intramuscularly, or subcutaneously; or orally, buccally, nasally, transmucosally, topically, in an ophthalmic preparation, or by inhalation, with a dosage ranging from about 0.1 to about 500 mg/kg of 10 body weight, alternatively dosages between 1 mg and 1000 mg/dose, every 4 to 120 hours, or according to the requirements of the particular drug. The methods herein contemplate administration of an effective amount of compound or compound composition to achieve the desired or stated effect. Typically, the pharmaceutical compositions of this invention will be administered from about 1 to about 6 times per day or alternatively, as a continuous 15 infusion. Such administration can be used as a chronic or acute therapy. The amount of active ingredient that may be combined with pharmaceutically excipients or carriers to produce a single dosage form will vary depending upon the host treated and the particular mode of administration. A typical preparation will contain from about 5% to about 95% active compound (w/w). Alternatively, such preparations may contain from about 20% to 20 about 80% active compound. Lower or higher doses than those recited above may be required. Specific dosage and treatment regimens for any particular patient will depend upon a variety of factors, including the activity of the specific compound employed, the age, body weight, general health status, sex, diet, time of administration, rate of excretion, drug combination, the 25 severity and course of the disease, condition or symptoms, the patient's disposition to the disease, condition or symptoms, and the judgment of the treating physician. Upon improvement of a patient's condition, a maintenance dose of a compound, composition or combination of this invention may be administered, if necessary. Subsequently, the dosage or frequency of administration, or both, may be reduced, as a 30 function of the symptoms, to a level at which the improved condition is retained when the symptoms have been alleviated to the desired level. Patients may, however, require intermittent treatment on a long-term basis upon any recurrence of disease symptoms. 71 WO 2008/033749 PCT/US2007/077977 Synthetic Methods A quinazoline derivative of the formula I, or a pharmaceutically-acceptable salt thereof, may be prepared by any process known to be applicable to the preparation of chemically-related compounds. Suitable processes for making certain intermediates include, 5 for example, those illustrated in European Patent Applications Nos. 0520722, 0566226, 0602851, 0635498, 0635507, US patent Nos. 5457105, 5,770599, US publication No. 2003/0158408 and reference such as, J. Med Chem. 2004, 47, 871-887. Necessary starting materials may be obtained by standard procedures of organic chemistry. The preparation of such starting materials is described within the accompanying non-limiting Examples. 10 Alternatively necessary starting materials are obtainable by analogous procedures to those illustrated which are within the ordinary skill of a chemist. The compounds and processes of the present invention will be better understood in connection with the following representative synthetic schemes that illustrate the methods by which the compounds of the invention may be prepared, which are intended as an 15 illustration only and not limiting of the scope of the invention. Scheme I 0 0 MeO COOH HCONH 2 Meo e h OH HO AczO/Py MeON~NH 2 eO N L-methionine WO NH eo NMeO N) 0101 0102 0103 0 C1 AcOoci AcO N H HN R2 POCAcO Nc& NNW MeO N MeO N 0106: R1 = F; R2 = C 0104 0105 0107:R1=HR2= - 0 N 0108: R1 F; R2 = CI 0111:R1 =H.R2= R1 Ri LiOH/H 2 O HN I R2 0 HN R2 HO O ON

ON

0 N) 0 N 0109: R1 = F; R2 = CI 0110: R1 = F; R2 = C1 0112:R1=H R2= = 0113: R1 = H, R2= R1

NH

2 OHHCI, KOHN HHN R2 HON N 0 N 72 WO 20081033749 PCT/US2007I077977 Scheme 2 0 0 H O C O O H C O H H O , A O IP y' A cO ,, ,

~N

2 H2 2 N' . N 0201 0202 0203 Cr Ri R I-CI HN R2 oc, AcOj( "N Ni: H 2 N R2 AcO = N N 0205: RI = F; R2 = Cl 0204 0206: Ri =H, R2 = - r 0207: Ri = F: R2 = Cl 0208: R1= H,R2-= Rl RI 0 LjHHOHNIZ(R2 OLN-R2 NHN R 0209r Ri = F; R2 =01l 0210: Ri = F;R2 =C 0211: Ri= H, R2= Rl 0212: R1= H,R2 =M

NI-

2 0H.HCI, KOH N HHN R2 HO' _? A N. el N 0 73 WO 20081033749 PCT/US2007/077977 Scheme 3 NCOOH HCONH 2 CI NH HNO,, AGO OCN H R~ Cl NH RO& N 0301 0302 0303 2N ' NH POCI 2 02N "N H 2 N R2 H ~ R 0304 0305 OX: 0306: RI = F, R2 = C 0307: Rl =H,R2- = RI RI 0 0 Fe/HCI H2 N 2 O"I 7'Ci H N R, N N 11 O0_5 ,- N I . N 0 N 0 00 N 0308: Ri F, R2 =Cl_ R 0309: Rl =H, R2 = 0310: R =F.R2 = C 0311:R1=H,R2= Ri 2HO HCI. KOH H H H ~ R HO J N " 0 00OC N 74 WO 2008/033749 PCT/US2007/077977 Scheme 4 0 0 -0 Br 0 + 1 eq. K 2 CO 3 I K2.,, 0 3 14 , 4 K 2 C0 3 0401 0402 O 0

SHN

3 O e Fe/HCI 0O %O H OAc 0~*O O 0403 0404 0 0

HCONH

2

)HCOONH

4 ON N O?* 0 ONH 2 O N 0405 0406 R1 R1 ClI POC13 0 N H 2 N R2 HNa R2 R NN N 0408 R 1 NH2OH H HNa R2 HONN Scheme 5 0 Y OH Br, Br O 0 Y O B + or 0501 HO r 0502 wherein Y is absent, Cl-Ce alky,

C

2 -C6 2kenyl, C 2

.C

6 aBkynyi 'CF 'CF HN Cl 0 Y Br K 2

CO

3 O HN C 0 N 0109 0502 0503 NH H

NH

2 OH N Y HO HNcCI 75 WO 2008/033749 PCTIUS2007/077977 Scheme 6 / F HSOHNO, F. F\ -ON e Or0N Br P~ 3 , Pd(OAC) 2 2 Br02 0602 0603 / Ci F AcO HN N.FKOH .

0 NH HN0105 AcO

H

2 NHN 0604 0605 060 F F . LiOH.H20 HN oAI2' r H N 0 .~ 'NO N0608 0607 F

NH

2 0H HN 'N HO NffhP'__ . ~N Scheme 7 AcO~ R2iH Re I- H R3 1,13 R 0105: R = CH3O- R N 2 0204: R = H 0701 R3 R3 HN N.INH O HN ' N. I N RTlK 0 N R2 ~' - R2 0 N 0 o N 0702 5 76 WO 20081033749 PCT/US2007/077977 Scheme 8 HNcRl HI 00 N R2 ~RI 0 0 Ol 0 IN H HNaR2 R CI or HO' N " 0803 R 0 N ~R-X I~R 0 N 0804 HN R2 0801: Rl F, R2=C -~ CN 0H~ ~ 0802: R1=HR2= -' 0 00 N Scheme 9 0 F F) HN" CI Nl~ HN Rl HO& Br"''" GINCl r N N NN 00902 0 N iN ~0 N. ON 0109 0901 0 IF H J IF R2 IN_______ R2 N ~'~ * N Ri0Ny 0 N-) 0 0903 Scheme 10 F ~ FF HN CI 2 HNa 2 CI 0ZB0H HN < C 0._ __ _ _ N "N KIH20N 0 ) Pd(OAc) 2 , PPH,, EtN, DMIF . N 0308 1001 1002 F -~0F --- 0 N 0 N " CH3CN ~ J Pd(0AC) 2 , PPH,, EtN, DMF . 0 N 0 N 1003 1004 NIH ~HO-NH 'Z 20 I- / I HN a CI MoOH 0 N"

N

0 N 77 WO 20081033749 PCTIUS2007/077977 Scheme I1I HNIC~CI -{}COO@~e HN cl N NN N ~~. ~ Pd(OAc),, PPH, EI 3 N, DMF I "0 N 0 N 1001 Pd/C H 1101 NH 2 0H 0 HN CI HO, HN<1 2 LC " 0 N "N H N N 1102 N 0eO HN Zl~C HON 4 "-N "0 N~ Schem 12 F120 0 HN

HNH

2 CI CI 0 NHN N N Ol0HN C MeOH N N " 0 N)~ H 0 N 1202 78 WO 2008/033749 PCT/US2007/077977 Scheme 13 0 0 0 H '~e o,' B jnO-' 0 HO 0--- K 2 C0 3 , DMF HO KC0 3 , DMF O OO 0401 1301 Ri 0 Ri 0 1 O 1 O 0 O- HNO 3 O O0 Fe, HCI HOAc NO 2 EtOH/H 2 O 1302 1303 RI 0 Ri 0 1 O 1 O O O HCONH 2 O O N POCl 3 ONHz

HCOONH

4 O O N 1304 1305 P R2 R1 C1 R2 RI HN R3 N H.N R3 O NH2OH &N ' &N 1306 1307 R2 RI HNI 2 R3 I HO, H 79 WO 2008/033749 PCT/US2007/077977 Scheme 14 0 0 Doc 0 Ic 0 HCONH N POC1 3 N N

NH

2 R H 2 - R N 1401 1402 1403 1404

H

2 N~a HN ~C HOL H / O~t: aF1 00 R NN 1405147 0 -KN 0 \NHN HNCIC 00 NN R N) 1407 WO 2008/033749 PCT/US2007/077977 Scheme 15 OH Bra F I Nl F Fe/HCI or H 2 /Pd-C 0 F

O

2 N Cl K 2 CO, CH 3 CN OzN Cl

H

2 N ci 1501 1502 1503 CI R NHNLF O F HN CI LiOH HN Cl AcO HO R R R 1 1504 1505 0 F 0 0 HN CI NH2OH, MeOH ^ o NN R N 1506 F 0 H A O HN C HO0 R N EXAMPLES The compounds and processes of the present invention will be better understood in 5 connection with the following examples, which are intended as an illustration only and not limiting of the scope of the invention. Various changes and modifications to the disclosed embodiments will be apparent to those skilled in the art and such changes and modifications including, without limitation, those relating to the chemical structures, substituents, derivatives, formulations and/or methods of the invention may be made without departing 10 from the spirit of the invention and the scope of the appended claims. EXAMPLE 1: Preparation of 2-(4-(3-Chloro-4-fluorophenylamino)-7 methoxyquinazolin -6-voxy)-N-hydroxvacetamide (Compound 1) Step la. 6,7-Dimethoxyquinazolin-4(3H)-one (Compound 0102) A mixture of methyl 2 -amino-4,5-dimethoxybenzoic acid 0101 (2.1 g, 10 mmol), 15 ammonium formate (0.63 g, 10 mmol) and formamide (7 ml) was stirred and heated to 81 WO 2008/033749 PCT/US2007/077977 190~200 'C for 2 hours. Then the mixture was cooled to room temperature. The precipitate was isolated, washed with water and dried to provide the title compound 0102 as a brown solid (1 .8g, 84.7%): LCMS: n/z 207[M+1 ]'; 'H NMR(DMSO) 6 3.87 (s, 3H), 3.89 (s, 311), 7.12 (s, 1H), 7.43 (s, 1H), 7.97 (s, 1H), 12.08 (bs, 1H). 5 Step 1b. 6-Hydroxy-7-methoxyquinazolin-4(3H)-one (Compound 0103) 6,7-Dimethoxyquinazolin-4(3H)-one (0102) (10.3 g, 50 mmol) was added portionwise to stirred methanesulphonic acid (68 ml). L-Methionone (8.6 g, 57.5 mmol) was then added and resultant mixture was heated to 150-160'C for 5 hours. The mixture was cooled to room temperature and poured onto a mixture (250 ml) of ice and water. The mixture was 10 neutralized by the addition of aqueous sodium hydroxide solution (40%). The precipitate was isolated, washed with water and dried to yield title compound 0103 as a grey solid (10g, crude): LCMS: m/z 193[M+1]f. Step ic. 3,4-Dihydro-7-methoxy-4-oxoquinazolin-6-yl acetate (Compound 0104) A mixture of 6-hydroxy-7-methoxyquinazolin-4(3H)-one (0103) (10 g crude), acetic 15 anhydride (100 ml) and pyridine (8ml) was stirred and heated to reflux for 3 hours. The mixture was cooled to room temperature and poured into a mixture (250ml) of ice and water. The precipitate was isolated and dried to yield the title product 0104 as a grey solid (5.8g, 50% two step overall yield): LCMS: n/z 235[M+1]*; 1H NMR(CDClt) 6 2.27 (s, 3H), 3.89 (s, 3H), 7.28 (s, I H), 7.72 (s, 1 H), 8.08 (d, 1 H), 12.20 (bs, 1 H). 20 Step 1d. 4 -Chloro-7-methoxyquinazolin-6-yl acetate (Compound 0105) A mixture of 3,4-dihydro-7-methoxy-4-oxoquinazolin-6-yl acetate (0104) (2.0 g, 8.5 mmol) and phosphoryl trichloride (20 ml) was stirred and heated to reflux for 3 hours. When a clear solution was obtained, the excessive phosphoryl trichloride was removed under reduced pressure. The residue was dissolved in dichloromethane (50m]) and the 25 organic layer was washed with aqueous NaHCO 3 solution (20mlx2) and brine (20mlx 1) and dried over MgSO 4 , filtered and evaporated to give the title product 0105 as a yellow solid (1.4g, 65%): LCMS: m/z 249[M+1]'; 'H NMR(CDCla) 6 2.40 (s, 3H), 4.03 (s, 3H), 7.44 (s, 1H), 7.90 (s, 1H), 8.95 (bs, 1H). Step le. 4

-(

3 -Chloro-4-fluorophenylamino)-7-methoxyquinazolin-6-yl acetate 30 hydrochloride (Compound 0108) A mixture of 4 -chloro-7-methoxyquinazolin-6-yl acetate (0105) (1.3 g, 5.1 mmol) and 3 chloro-4-fluorobenzenamine 0106 (1.5 g, 10.2 mmol) in isopropanol (45ml) was stirred and heated to reflux for 3 hours. The mixture was cooled to room temperature and resulting 82 WO 2008/033749 PCT/US2007/077977 precipitate was isolated. The solid was then dried to give the title compound 0108 as a light yellow solid (1.6 g, 79%): LCMS: ni/z 362[M+1]-; IH NMR(DMSO) 3 2.36 (s, 3H), 3.98 (s, 3H), 7.49 (s, I H), 7.52 (d, 111), 7.72 (m, 1H), 8.02 (dd, 1 H), 8.71 (s, I 1), 8.91 (s, 1 H), 11.4 (bs, 1H). 5 Step iLf. 4-(3-Chloro-4-fluorophenylamino)-7-methoxyquinazolin-6-ol (Compound 0109) A mixture of compound (0107) (1.41 g, 3.5 mmol), LiOH H 2 0 (0.5 g, 11.7 mmol) in methanol (100 ml) and H 2 0 (100 ml) was stirred at room temperature for 0.5 hour. The mixture was neutralized by addition of dilution acetic acid. The precipitate was isolated and dried to give the title compound 0109 as a grey solid (1.06g, 94%): LCMS: m/z 320[M+1]*; 10 'H NMR(DMSO) 6 3.99 (s, 3H), 7.20 (s, 1H), 7.38 (t, IH), 7.75 (s, 1H), 7.81 (m,1H), 8.20 (m,1H), 8.46 (s,lH), 9.46 (s,1H), 9.68 (s,1H). Step 1g. Ethyl 2-(4-(3--chloro- 4 -fluorophenylamino)-7-methoxyquinazolin-6-yloxy)acetate (Compound 0110-1) A mixture of compound 0109 (300 mg, 0.94 mmol) and Ethyl 2-bromoacetate (163 mg, 15 0.98 mmol) and potassium carbonate (323 mg, 2.35 mmol) in N,N-dimethylformamide(6 ml) was stirred and heated to 40 for 30 minutes. The reaction process was monitored by TLC. The mixture was filtrated. The filtration was concentrated under reduce pressure. The residues was wash with diethyl ether and dried to give the title compound 0110-1 as a yellow solid (280 mg, 74'%): LCMS: n/z 406[M+1 ]'; 'H NMR(DMSQ) 6 1.23(t, 3H), 3.96 20 (s, 3H), 4.20 (q, 2H), 4.95 (s, 2H), 7.24 (s, IH), 7.44 (t, 1H), 7.75 (m, 1H), 7.82 (s,1H), 8.10 (dd, 1H), 8.51 (s, IH), 9.54 (s, 1H). Step 1h. 2-(4-(3-Chloro- 4 -fluorophenylamino)-7-methoxyquinazolin-6-yloxy)-N hydroxyacetamide (Compound 1) To a stirred solution of hydroxyamine hydrochloride (4.67 g, 67mmol) in methanol 25 (24ml) at 0 0 C was added a solution of potassium hydroxide (5.6 1g, 100mmol) in methanol (14ml). After addition, the mixture was stirred for 30 minutes at 0 0 C, and was allowed to stand at low temperature. The resulting precipitate was isolated, and the solution was prepared to give free hydroxyamine. The above freshly prepared hydroxyamine solution (1.4 ml, 2.4mmol) was placed in 5ml 30 flask. Compound 0110-1 (250 mg, 0.6mmol) was added to this solution and stirred at 0 0 C for 10 minutes, and raise to room temperature. The reaction process was monitored by TLC. The mixture was neutralized with acetic acid. The mixture was concentrated under reduce pressure. The residue was purified by preparation HPLC. To give the title compound 1 as a 83 WO 2008/033749 PCT/US2007/077977 grey solid (50mg, 21%): LCMS: m/z 393[M+1]; 'HNMR(DMSQ) 6 3.96(s, 3H), 4.62 (s, 2H), 7.24 (s, 1H), 7.45 (t, 1H), 7.78 (in, IH), 7.86 (s,IH), 8.10 (dd,IH), 8.52 (s,IH), 9.07 (s, 1 H), 9.57 (s, 1 H), 10.80 (s, 11). 5 EXAMPLE 2: Preparation of 4-(4-(3-Chloro-4-fluorophenylamino)-7 methoxvquinazolin- 6-Vloxv)-N-hvdroxybutanamide (Compound 3) Step 2a. Ethyl 4-(4-(3-chloro-4-fluorophenylamino)-7-methoxyquinazolin-6 yloxy)butanoate (Compound 0110-3) The title compound 0110-3 was prepared as a yellow solid (220mg, 80.5%) from 10 compound 0109 from step If (200mg, 0.63mmol) and ethyl 4-bromobutyrate (135mg, 0.69mmol) using a procedure similar to that described for compound 0110-1 (example 1): LCMS: n/z 434[M+1]-; H NMR(CDCl.) 6 1.36 (t, 3H), 2.23 (m,2H), 2.57 (t, 2H), 4.03 (s, 3H), 4.32 (in, 4H), 7.15 (t, 1H), 7.25 (m, 1H), 7.87 (s, 1H), 8.00 (m,2H), 8.15 (bs,1H), 8.57 (s,1H). 15 Step 2b. 4

-(

4

-(

3 -Chloro-4-fluorophenylamino)-7-methoxyquinazolin-6-yloxy)-N hydroxybutanamide (Compound 3) The title compound 3 was prepare as a grey solid (25 mg, 12%) from compound 0110-3 (200mg, 0.23mmol) using a procedure similar to that described for compound I (Example 1): LCMS: m/z 421 [M+-1]'; 'H NMR(DMSO): 6 2.06 (m,2H), 2.22 (t, 2H), 3.95 (s, 3H), 20 4.15 (t, 2H), 7.21 (s, 1H), 7.43 (t, 1H), 7.83 (s, 2H), 8.14 (dd, 1H), 8.51 (s, 1H), 8.75 (s, 1H), 9.56 (s, 1H), 10.50 (s, 1H). EXAMPLE 3: Preparation of 7-(4-(3-Chloro-4-fluorophenylamino)-7 methoxyquinazolin -6-vloxv)-N-hydroxvhexanamide (Compound 5) Step 3a. Ethyl 6-(4-(3-chloro-4-fluorophenylamino)-7-methoxyquinazolin-6 25 yloxy)hexanoate (Compound 0110-5): The title compound 0110-5 was prepared as a yellow solid (510 mg, 68%) from compound 0109 from step If (510mg, 1.6mmol) and ethyl 6-bromohexanoate (430mg, 1.9mmol) using a procedure similar to that described for compound 0110-1 (Example 1): LCMS: m/z 462[M+1]-; 1 H NMR(CDCl 3 ): 6 1.24 (t, 3H),1.55 (m, 2H), 1.74 (m, 2H), 1.91 30 (m, 2H), 2.38 (m, 2H), 3.97 (s, 3H), 4.13 (m, 4H), 7.15 (t, 1H), 7.25 (in, 2H), 7.60 (m, 1H), 7.86 (in, 1H), 7.91 (dd, 1H), 8.61 (s, 1H). Step 3b. 7-( 4 -(3-Chloro-4-fluorophenylamino)-7-methoxyquinazolin-6-yloxy)-N hydroxyhexanamide (Compound 5) 84 WO 2008/033749 PCT/US2007/077977 The title compound 5 was prepared as a grey solid (100 mg, 34%) form compound 0110-5 (305mg, 0.66mmol) using a procedure similar to that described for compound I (Example 1): m.p.206.6~207.1 'C (dec); LCMS: m/z 449[M+1] ; H NMR(DMSQ) 6 1.44 (in, 2H), 1.64 (in, 2H), 1.82 (in, 2H), 1.99 (t, 2H), 3.93 (s, 3H), 4.12 (t, 2H), 7.19(s, 1H), 5 7.43 (t, 1H), 7.79 (m, 2H), 8.12 (dd, 1H), 8.49 (s, 1H), 8.68 (s, 1H), 9.53 (s, 1H), 10.37 (s, 1H). EXAMPLE 4: Preparation of 7 -(4-(3-Chloro-4-fluorophenylamino)-7 methoxciuinazolin-6-yloxy)-N-hydroxvheptanamide (Compound 6) 10 Step 4a. Ethyl 7-(4-(3-chloro- 4 -fluorophenylamino)-7-methoxyquinazolin-6 yloxy)heptanoate (compound 0110-6) The title compound 0110-6 was prepared as a yellow solid (390 mg, 53%) from compound 0109 from step If (512mg, 1.6mmol) and ethyl 7-bromoheptanoate(43 8mg, 1.8mmol) using a procedure similar to that described for compound 0110-1 (Example 1): 15 LCMS: m/z 476[M+1-l; 1 H NMR(CDCla) 6 1.24 (t, 3H),1.43 (m,4H), 1.66 (m,2H), 1.88 (m,2H), 2.32 (t, 2H), 3.97 (s, 3H), 4.07 (t, 2H), 4.12 (q, 2H), 7.15 (t, 1H), 7.23 (t, 2H), 7.66 (in, 1H), 7.75 (m,1H), 7.87 (dd, 1H), 8.65 (s,1H). Step 4 b.

7

-(

4

-(

3 -Chloro-4-fluorophenylamino)-7-methoxyquinazolin-6-yloxy)-N hydroxyheptanamide (compound 6) 20 The title compound 6 was prepared as a grey solid (80 mg, 25%) from compound 0110-6 (323mg, 0.68mmol) using a procedure similar to that described for compound 1 (Example 1): m.p.180.8-182.3 C (dec); LCMS: m/z 463[M+1]+; 1 H NMR(DMSO) 6 1.34 (m,2H), 1.50 (m,4H), 1.81 (m,2H), 1.96 (t, 2H), 3.92 (s, 3H), 4.11 (t, 2H), 7.18 (s, 1H), 7.43 (t, 1H), 7.78 (in, 2H), 8.12 (dd, 1 H), 8.48 (s, 1 H), 8.64 (s, 1 H), 9.50 (s, I H), 10.33 (s, 1 H). 25 EXAMPLE 5: Preparation of 2

-(

4

-(

3 -ethynylphenyiamino)7methoxycuinazolin6 yloxy)-N- hydroxacetamide (Compound 7) Step 5a. 4

-(

3 -Ethynylphenylamino)-7-methoxyquinazolin-6-yl acetate Hydrochloride (Compound 0111) 30 A mixture of 4 -chloro-7-methoxyquinazolin-6-yl acetate (0105) (2.6 g, 10.2 mmol) and 3-ethynylbenzenamine (0107) (2.4 g, 20.5 mmol) in isopropanol (100 ml) was stirred and heated to reflux for 3 hours. The mixture was cooled to room temperature. The precipitate was isolated and dried to give the title compound 0111 as a yellow solid (2.6g, 68%): 85 WO 20081033749 PCT/US2007/077977 LCMS: m/z 334[M+1]~; 1 HNMR(DMSO) 62.39 (s,3H), 3.17 (s, 1H), 3.98 (s, 3H), 7.35 (in, 1H), 7.40 (s, IH), 7.47 (m, IH), 7.72 (m, IH), 7.90 (s, IH), 8.57 (sIH), 8.87 (s,IH), 10.99 (bs, 1H). Step 5b. 4-(3-Ethynylphenylamino)-7-methoxyquinazolin-6-ol (Compound 0112) 5 A mixture of compound 0111 (2.0 g, 5.4 mmol) and LiOH H 2 0 (0.75 g, 17.9 mmol) in methanol (100 ml) and H 2 0 (100 ml) was stirred at room temperature for 0.5 hour. The mixture was neutralized by addition of dilution acetic acid. The precipitate was isolated and dried to give the title compound 0112 as a grey solid (1.52g, 96%): LCMS: m/z 292[M+1],; 1 H NMR(DMSO.) 6 3.17 (s, 1H), 3.98 (s, 3H), 7.18 (d, 1H), 7.21 (s, 1H), 7.37 (t, 1H), 7.80 10 (s, 1H), 7.90 (d, 1H), 8.04 (m, 1H), 8.47 (s,LH), 9.41 (s, 1H), 9.68 (bs, 1H). Step 5c. Ethyl 2-(4-(3-ethynylphenylamino)-7-methoxyquinazolin-6-yloxy)acetate (Compound 0113-7) The title compound 0113-7 was prepared as a yellow solid (450 mg, 69%) from compound 0112 (500mg, 1.72mmol) and ethyl 2-bromoacetate (300mg, 1.8mmol) using a 15 procedure similar to that described for compound 0110-1 (Example 1) : LCMS: m/z 378[M+1f]; H NMR(DMSO) 6 1.22(t, 3H), 3.97 (s, 3H), 4.21(q,2H), 4.97(t,2H), 7.22(d, 1H), 7.24(s, 1H), 7.42(t, 1H), 7.84(m,2H), 7.86(d,1H), 7.96(s,1H), 8.51(s.1H). Step 5d. 2-(4-(3-Ethynylphenylamino)-7-methoxyquinazolin-6-yloxy)-N hydroxyacetamide (Compound 7) 20 The title compound 7 was prepared as a grey solid (100 mg, 23%) from compound 0113-7 (448mg, 1.2mmol) using a procedure similar to that described for compound 1 (Example 1): LCMS: m/z 365[M+1]+; 'H NMR(DMSO) 6 4.00 (s, 3H), 4.26 (s, 1H), 4.65(s, 2H), 7.27 (s, 1H), 7.37 (d, 1H), 7.49 (t, 1H), 7.73 (d, 1H), 7.85(s, 1H), 8.03 (s,1H), 8.78 (s,1 H), 9.17 (bs,1H), 10.60 (s, 1UH), 10.85 (s, 1H). 25 EXAMPLE 6: Preparation of 4-(4-(3-ethynylphenylamino)-7-methoxyquinazolin-6 Yloxy)-N- hydroxybutanamide (Compound 9) Step 6a. Ethyl 4-(4-(3-ethynylphenylamino)-7-methoxyquinazolin-6-yloxy)butanoate (Compound 0113-9) 30 The title compound 0113-9 was prepared as a yellow solid (438mg, 59%) from compound 0112 (500mg, 1.72mmol) and ethyl 4-bromobutyrate (349mg, 1.8mmol) using a procedure similar to that described for compound 0110-1 (Example 1): LCMS: n/z 406[M+1]+; 'H NMR(CDCI ) 6 1.37 (t, 3H), 2.34 (in, 2H), 2.56 (t, 2H), 3.07 (s, 1H), 4.03 86 WO 2008/033749 PCT/US2007/077977 (s, 3H), 4.32 (m,4H), 7.21 (m, 1H), 7.25 (s, 1H), 7.36 (t, 1H), 7.94 (s, 1H), 7.97 (m, 1H), 8.20 (s,1H), 8.28 (m, IH), 8.70(s, 1H). Step 6b. 4-(4-(3-Ethynylphenylamino)-7-methoxyquinazolin-6-yloxy)-N hydroxybutanamide (Compound 9) 5 The title compound 9 was prepared as a grey solid (60 mg, 31%) from compound 0113-9 (200mg, 0.49mmol) using a procedure similar to that described for compound 1 (Example 1): LCMS: n/z 393 [M+1]'; 'H NMR(DMSO) 6 2.06 (m, 2H), 2.22 (t, 2H), 3.30 (s, IH), 3.95 (s, 3H), 4.16 (t, 2H), 7.19 (m, 2H), 7.40 (t, 1H), 7.85 (s, 1H), 7.91 (d, 1H), 8.02 (s,lfH), 8.51 (s,1H), 8.74 (s,IH), 9.49 (s, 1H), 10.49 (s, 1H). 10 EXAMPLE 7: Preparation of 6-(4-(3-ethynylphenylamino)-7-methoxyquinazolin-6 yloxy)-N- hydroxyhexanamide (Compound 11) Step 7a. Ethyl 6-(4-(3-ethynylphenylamino)-7-methoxyquinazolin-6-yloxy)hexanoate (Compound 0113-11) 15 The title compound 0113-11 was prepared as yellow solid (543 mg, 73%) from compound 0112 from step 5b (500mg, 1. 72mmol) and ethyl 6-bromohexanoate(40 1mg, 1.8mmol) using a procedure similar to that described for compound 0110-1 (Example 1): LCMS: m/z 434[M+1]; H NMR(CDCla) 6 1.24(t, 3H), 1.53(m, 2H), 1.72(m, 2H), 1.90(m, 2H), 2.37(t, 3H), 3.08(s, 1 H), 3.97 (s, 31H), 4.10(m,4H), 7.19(s, 1 H), 7.25(m, 2H), 7.34(t, 20 1H), 7.67(s, 1H), 7.78(m, 1H), 7.84(m, 1H), 8.67(s, 1H). Step 7b. 6-(4-(3-Ethynylphenylamino)-7-methoxyquinazolin-6-yloxy)-N hydroxyhexanamide (Compound 11) The title compound 11 was prepared as a grey solid (110 mg, 41%) from compound 0113-11 (275mg, 0.63mmol) using a procedure similar to that described for compound 1 25 (Example 1): m.p.193.4-195.8 C (dec); LCMS: n/z 421 [M+1]+; 'H NMR(DMSQ) 6 1.44 (m, 2H), 1.60 (m, 2H), 1.84 (m, 2H), 1.99(t, 2H), 3.93 (s, 3H), 4.13 (t, 2H), 4.19(s, 1H), 7.19 (m, 2H), 7.40 (t, 1H), 7.81 (s, 1H), 7.88 (d, 1H), 7.98 (s,1H), 8.49 (s,1H), 8.68 (s,1H), 9.47 (s, IH), 10.39 (s, 1H). 30 EXAMPLE 8: Preparation of 7-(4-(3-ethynylphenylamino)-7-methoxyquinazolin-6 yloxy)-N- hydroxyheptanamide (Compound 12) Step 8a. Ethyl 6 -(4-(3-ethynylphenylamino)-7-methoxyquinazolin-6-yloxy) heptanoate (Compound 0113-12) 87 WO 2008/033749 PCT/US2007/077977 The title compound 0113-12 was prepared as a yellow solid (305 mg, 84%) from compound 0112 from step 5b (247mg, 0.85mmol) and ethyl 7-bromohepanoate (211mg, 0.89mmol) using a procedure similar to that described for compound 0110-1 (Example 1): LCMS: 448 [M+1]*; 1 H NMR (CDC 3 ): 61.15 (t, J= 7.5 Hz, 3H), 1.33 - 1.60 (in, 6H), 1.81 5 (m, 2H), 2.28 (t, J= 7.5 Hz, 2H), 3.92 (s, 3H), 4.03 (q, J= 7.2 Hz, 2H), 4.12 (t, J= 6.6 Hz, 2H), 4.18 (s, 1H), 7.19 (m, 2H), 7.39 (t, J= 7.8 Hz, 1H), 7.80 (s, 1H), 7.89 (d, J= 8.1 Hz, IH), 7.97 (s, 1H), 8.48 (s, 1H), 9.44 (s, 1H). Step 8b. 7-(4-(3-Ethynylphenylamino)-7-methoxyquinazolin-6-yloxy)-N hydroxyheptanamide (Compound 12) 10 The title compound 12 was prepared as a grey solid (100 mg, 41%) from compound 0113-12 (250mg, 0.56mmol) using a procedure similar to that described for compound I (Example 1): m.p.171.8-177.2 'C (dec); LCMS: 435 [M+l]+; 'H NMR (DMSO-d):61.36 (in, 2H), 1.52 (in, 4H), 1.83 (in, 2H), 1.97 (in, 2H), 3.94 (s, 3H), 4.14 (t, J= 6.3 Hz, 2H), 4.20 (s, 1H), 7.21 (in, 2H), 7.41 (t, J= 8.1 Hz, 1H), 7.83 (s, tH), 7.90 (d, J=8.1 Hz, 1H), 15 8.00 (s, 1H), 8.50 (s, 1H), 8.66 (s, 1H), 9.48 (s, lH), 10.35 (s, 1H). EXAMPLE 8 (METHOD 2): Preparation of 7-(4-(3-ethynylphenylamino)-7 methoxyquinazolin-6-yloxy)-N-hydroxyheptanamide (Compound 12) Step 8a'. Ethyl 3-hydroxy-4-methoxybenzoate (Compound 0402-12) 20 To a solution of ethyl 3, 4-dihydroxybenzoate 0401 (12.52 g, 68.7 mmol) in DMF (50 mL) was added potassium carbonate (9.48 g, 68.7 mmol). After the mixture was stirred for 15 minutes, a solution of iodomethane (9.755 g, 68.7 mmol) in DMF (10 mL) was added dropwise. The reaction mixture was stirred at 20'C for 24 hours. After reaction the mixture was filtered, and the filtrate was concentrated. The residue was dissolved in 25 dichloromethane and washed with brine. The organic phase was dried over sodium sulfate, filtered and concentrated in vacuo to give crude product. The crude product was purified by column chromatography to give the title compound 0402-12 as a white solid (7.1 g, 53%): LCMS: 197 [M+1] +, 'H NMR (DMSO-d): 6 1.29 (t, J= 6.6 Hz, 3H), 3.83 (s, 3H), 4.25 (q, J= 6.6 Hz, 2H), 7.00 (d, J= 8.4 Hz, 1H), 7.38 (d, J= 1.8 Hz, 1H), 7.43 (dd, J = 8.4 Hz, 2.1 30 Hz, 1H1), 9.36 (s, 1H). Step 8b'. Ethyl 3 -(7-ethoxy-7-oxoheptyloxy)-4-methoxybenzoate (Compound 0403-12) A mixture of compound 0402-12 (6.34 g, 32.3 mmol), ethyl 7-bromoheptanoate (7.66 g, 32.3 mmol) and potassium carbonate (13.38 g, 96.9 mmol) in DMF (80 mL) was stirred at 88 WO 2008/033749 PCT/US2007/077977 60'C for 3 hours. After reaction the mixture was filtrated. The filtrate was concentrated in vacuo and the residue was dissolved in dichloromethane and washed with brine twice. The organic phase was dried over sodium sulfate, filtered and concentrated to give the title product 0403-12 as a white solid (9.87 g, 86.7%): LCMS: 353 [M+1], 'H NMR (DMSO 5 d 6 ): 61.17 (t, J= 6.9 Hz, 3H), 1.31 (t, J= 7.2 Hz, 3H) 1.39 (in, 4H), 1.54 (in, 2H), 1.72 (in, 2H), 2.29 (t, J= 7.2 Hz, 2H), 3.83 (s, 3H), 3.98 (t, J= 7.2 Hz, 2H), 4.06 (q, J= 6.9 Hz, 2H), 4.29 (q, J= 7.2 Hz, 2H), 7.06 (d, J= 8.4 Hz, 1H), 7.42 (d, J= 1.8 Hz, 1H), 7.57 (dd, J= 8.4 Hz, 1.8 Hz, 1H). Step 8c'. Ethyl 5-(7-ethoxy-7-oxoheptyloxy)-4-methoxy-2-nitrobenzoate (Compound 10 0404-12) Compound 0403-12 (9.87 g, 28.0 mmol) was dissolved in acetic acid (20 mL) and stirred at 20*C. Fuming nitric acid (17.66 g, 280.0 mmol) was added slowly dropwise. The mixture was stirred at 20 0 C for 1 hour. After reaction the mixture was poured into ice-water and extracted with dichloromethane twice. The combined organic phase was washed with 15 brine, aqueous NaHCO 3 solution and brine. The combined organic phase was dried over sodium sulfate, filtered and concentrated to give the title product 0404-12 as a yellow solid (10.75 g, 96.4%): LCMS: 398 [M+1)-, 'H NMR (DMSO-d): 61.17 (t, J= 7.2 Hz, 3H), 1.27 (t, J= 7.2 Hz, 3H), 1.38 (in, 4H), 1.53 (m, 2H), 1.74 (m, 2H), 2.29 (t, J= 7.2 Hz, 2H), 3.91 (s, 3H), 4.03 (q, J= 7.2 Hz, 2H), 4.08 (t, J= 6.3 Hz, 2H), 4.30 (q, J= 7.2 Hz, 2H), 7.29 (s, 20 1H), 7.63 (s, 1H). Step 8d'. Ethyl 2 -amino-5-(7-ethoxy-7-oxoheptyloxy)-4-methoxybenzoate (Compound 0405-12) A mixture of 0404-12 (10.75 g 27.0 mmol), ethanol (120 mL), water (40 mL) and hydrogen chloride (4 mL) was stirred to form a clear solution. The iron powder (15.16 g, 25 27.0 mmol) was added batchwise. The mixture was stirred at reflux for 30 min, and was then cooled to room temperature, adjusted pH to 8 with 10% sodium hydroxide solution, and filtered. The filtrate was concentrated to remove ethanol and extracted with dichloromethane twice. The combined organic phase was washed with brine and dried over sodium sulfate, filtered and concentrated to give the title product 0405-12 as a yellow solid 30 (8.71 g, 87.8%): LCMS: 368 [M+1] t , 'H NMR (DMSO-d): 6 1.17 (t, J= 7.2 Hz, 3H), 1.28 (t, J= 7.2 Hz, 3H), 1.37 (m, 4H), 1.53 (in, 2H), 1.66 (in, 2H), 2.29 (t, J= 7.2 Hz, 2H), 3.74 (s, 3H), 3.78 (t, J= 6.9 Hz, 2H), 4.06 (q, J= 7.2 Hz, 2H), 4.22 (q, J= 7.2 Hz, 2H), 6.35 (s, 1H), 6.44 (s, 2H), 7.15 (s, 1H). 89 WO 2008/033749 PCT/US2007/077977 Step 8e'. Ethyl 7-(7-methoxy-4-oxo-3, 4-dihydroquinazolm-6-yloxy) heptanoate (Compound 0406-12) A mixture of compound 0405-12 (8.71 g, 23.7 mmol), ammonium formate (1.48 g, 23.7 mmol) and formamide (40 mL) was stirred at 180 C for 3 hours. After reaction the mixture 5 was cooled to room temperature. The formamide was removed under reduce pressure, and the residue was dissolved in dichloromethane and washed with brine. The organic phase was dried over sodium sulfate, filtered and concentrated to give the title product 0406-12 as a pale white solid (8.18g, 99%): LCMS: 349 [M+1]*, 'H NMR (DMSO-d 6 ): 61.17 (t, 1= 6.9 Hz, 3H), 1.38 (in, 4H), 1.55 (in, 2H), 1.75 (m, 2H), 2.29 (t, J= 7.2 Hz, 2H), 3.90 (s, 10 3H), 4.05 (m, 4H), 7.13 (s, 1H), 7.42 (s, 1H), 7.97 (d, J= 3.6 Hz, lH), 12.07 (s, 1H). Step 8f'. Ethyl 7

-(

4 -chloro-7-methoxyquinazolin-6-yloxy) heptanoate (Compound 0407-12) A mixture of product 0406-12 (8.18 g, 23.5 mmol) and phosphoryl trichloride (50 mL) was stirred at reflux for 4 hours. After reaction the excessive phosphoryl trichloride was removed under reduced pressure. The residue was dissolved in dichloromethane and washed 15 with water, aqueous NaHCO 3 solution and brine. The organic phase was dried over sodium sulfate, filtered and concentrated to give the title product 0407-12 as a yellow solid (5.93 g, 69.7%): LCMS: 367 [M+1]', 'H NMR (DMSO-d): 61.17 (t, J= 6.9 Hz, 3H), 1.38 (in, 4H), 1.54 (in, 2H), 1.81 (in, 2H), 2.30 (t, J= 7.2 Hz, 2H), 4.02 (s, 3H), 4.06 (q, J= 6.9 Hz, 2H), 4.18 (t, J= 6.3 Hz, 2H), 7.37 (s, 1H), 7.45 (s, 1H), 8.87 (s, 1H). 20 Step 8g'. Ethyl 7

-(

4

-(

3 -ethynylphenylamino)-7-methoxyquinazolin-6-yloxy) heptanoate (Compound 0408-12) A mixture of product 0407-12 (5.93 g, 16.4 mmol) and 3-ethynylbenzenamine (1.92 g, 16.4 mmol) in isopropanol (80 mL) was stirred at reflux 4 hours. After reaction the mixture was cooled to room temperature and resulting precipitate was isolated, washed with 25 isopropanol and ether, and dried to give the title compound 0408-12 as a yellow solid (4.93 g, 67.1%): LCMS: 448 [M+1]-, 'H NMR (DMSO-d 6 ): 61.16 (t, J= 7.2 Hz, 3H), 1.36 1.59 (m, 6H), 1.80 (m, 2H), 2.29 (t, J= 7.2 Hz, 2H), 3.93 (s, 3H), 4.04 (q, J= 6.9 Hz, 2H), 4.13 (t, J= 6.6 Hz, 2H), 4.19 (s, 1H), 7.20 (m, 2H), 7.39 (t, J= 7.8 Hz, 1H), 7.81 (s, 1H), 7.89 (d, J= 8.4 Hz, I H), 7.97 (s, 1 H), 8.48 (s, 1 H), 9.45 (s, 1 H). 30 Step Sh'. 7

-(

4

-(

3 -ethynylphenylamino)-7-methoxyquinazolin-6-yloxy)-N hydroxyheptanamide (Compound 12) The freshly prepared hydroxylamine solution (30 mL, 110 mmol) was placed in 50 mL flask. Compound 0408-12 (4.93 g, 11.0 mmol) was added to this solution and stirred at 90 WO 2008/033749 PCT/US2007/077977 25"C for 24 hours. After reaction the mixture was neutralized with acetic acid, and the resulting precipitate was isolated, washed with water, and dried to give the title compound 12 as a white solid (3.99 g, 83.6%): mp 174.1~177.2 C. LCMS: 435 [M+1]', 'H NMR (DMSO-d): 61.36 (in, 2H), 1.52 (in, 4H), 1.83 (i, 2H), 1.98 (in, 2H), 3.94 (s, 3H), 4.14 (t, 5 J= 6.6 Hz, 2H), 4.20 (s, 1H), 7.21 (m, 2H), 7.41 (t, J= 7.8 Hz, 1H), 7.80 (s, 1H), 7.90 (d, J =7.8 Hz, 1H), 8.00 (s, 1H), 8.50 (s, 1H), 8.66 (s, 1H), 9.48 (s, 1H), 10.35 (s, 1H). Example 9: Preparation of 2

-(

4 -(3-chloro-4-fluorophenvlamino) quinazolin-6-yloxy) N-hydroxyacetamide (Compound 13) 10 Step 9a. 6 -Hydroxyquinazolin-4(3H)-one (compound 0202) To a solution of 2 -amino-5-hydroxybenzoic acid 0201 (30.6 g, 0.2 mol) in formamide was stirred and heated to 190 'C for 0.5 h. The mixture was allowed to cool to room temperature. The precipitate was isolated, washed with ether and dried to obtain title compound 0202 (3 2 g, brown solid, yield: 99%): LC-MS n/z 163 [M+1]; 15 'H NMREDMSO O7.250 dd,1HEO,7.40d,1H-],7.460 d,1H O,7.880s,1H. Step 9b. 3, 4 -Dihydro-4-oxoquinazolin-6-yl acetate (Compound 0203) A mixture of compound 0202 (30.0 g, 0.185 mol) and pyridine (35 ml) in acetic anhydride (275 ml) was stirred and heated at 100 "C for 2 hours. The reaction was poured into a mixture of ice and water (500 ml). The precipitate was isolated, washed with water and 20 dried to obtain the title compound 0203 (24 g, pale white solid, yield: 61%): LC-MS m/z 205 [M+1]; 1H-NMRODMSO[O6 2.32 (s,3H),7.50EOdd,1H 0,7.80O d,1H , 7.98(s,1H),8.02 Os,1H[O. Step 9c. 4 -Chloroquinazolin-6-yl acetate (Compound 0204) A mixture of compound 0203 (20.0 g, 0.1 mol) in POC1 3 (150 ml) was stirred and 25 heated to reflux for 2 hours. The reaction was evaporated and the residue was partitioned between ethyl acetate and a saturated aqueous NaHCO 3 solution. The organic phase was washed with water, dried over Na 2

SO

4 and evaporated. The mixture was purified by column chromatography (silica gel, elution: 1:2= ethyl acetate/petroleum) to obtained the title compound 0204 (7.5g, white solid, yield: 35%): LC-MS n/z 223 [M+1]; 'H 30 NMROCDCI3EO62.40(s,3H),7.74DOdd, lHIO, 8.OODd, 1H,8.09 (d,1H), 9.05LOs,1H O. Step 9d. 4

-(

3 -Chloro-4-fluorophenylamino)quinazolin-6-yl acetate (Compound 0207) A mixture of 0204 (1.0 g, 4.5 mmol) and 3-chloro-4-fluorobenzenamine 0205 (0.7 g, 5.0 mmol) in isopropanol (45ml) was stirred and heated at 90 C for 1 hours. The reaction was 91 WO 20081033749 PCT/US2007/077977 cooled to room temperature and the precipitate was isolated. The solid was washed in turn with isopropanol and methanol, dried to provide the title compound 0207 (1.3 g, pale yellow solid, yield: 87%): LC-MS mn/z 332 [M+1]; 11-NMR - DMSO7o2.37ns,31 -I,7.54nt, 1H O, 7.75(m,1H),7.94(dd, 1H),7.990 s, 1H [,8.02(m,1H),8.640 s,1H O,8.95(s,1H). 5 Step 9e. 4 -(3-Chloro-4-fluorophenylamino)quinazolin-6-ol (Compound 0209) A mixture of 0207 (0.8 g,2.6 mmol) and lithium hydroxide monohydrate (0.13 g, 3.2 mmol) in methanol (10 ml)/water (15 ml)was stirred at room temperature for 1 hour. The pH was adjusted to 4 with acetic acid and filtered. The collected yellow solid was washed by water and dried to obtained title compound 0209 (0.6g, yellow solid, yield: 88%): LC 10 MS mn/z 290 [M+1]; 1 H-NMRLODMSO067.420 s,lH), 7.450m,1HI, 7.70 Ld,1H O,7.76(s,1H),7.86[Om,1HEO,8.24(q, lH),8.48[1s,1H O,9.61(s,1H). Step 9f. Ethyl 2

-(

4

-(

3 -chloro-4-fluorophenylamino)quinazolin-6-yloxy)acetate (Compound 0210-13) A mixture of 0209 (0.2 g, 0.77 mmol), ethyl 3-bromopropanoate (0. 14g, 0.85mmol) and 15 K 2 C0 3 (0.8g, 5.8mmol) in DMF (15ml) was stirred and heated to 80 C for 2 hours . The reaction was filtered and the filtrate was evaporated. The resulting solid was washed with ether to obtain the title compound 0210-13 (0.2 g,yellow solid, yield: 75%): mp161-163 'C; LC-MS in/z 376 [M+1]; 1H-NMRL DMSO J61.20 Jt, 3HLJ,4.20 uq, 2HLU, 4.96]s,2HF,7.45(t,1 H),7.55 Fdd, IHn,7.78(m,2H), 7.947d, 1HF],8.16(dd, I H),8.54(s,1 H), 20 9.69(s.1H). Step 9g. 2-(4-(3-Chloro- 4 -fluorophenylamino)quinazolin-6-yloxy)-N-hydroxyacetamide (Compound 13) To a stirred solution of hydroxyamine hydrochloride (4.67 g, 67mmol) in methanol (24ml) at 0C was added a solution of potassium hydroxide (5.61g, 100mmol) in methanol 25 (14ml). After addition, the mixture was stirred for 30 minutes at 0 'C, and was allowed to stand at low temperature. The resulting precipitate was isolated, and the solution was prepared to give free hydroxyamine. Take above solution (1.4ml, 2.4mmol) into 5ml flask. Compound 0210-13 (0.1 g, 0.29mmol) was added into this solution and stirred at 0 C for 10 minutes, and then allowed 30 to warm to room temperature. The reaction process was monitored by TLC. The mixture was adjusted pH to 6 with acetic acid and then concentrated under reduce pressure. The residue was purified by preparation HPLC eluted by methanol/water. The band containing the product was collected. The solvent was evaporated to obtain title compound 13 (30 mg, 92 WO 20081033749 PCT/US2007/077977 yellow solid, yield: 29%): LC-MS m/z 363 [M+1]; 1H NMRLDMSOL64.64Us,2H U,7.46 Jt,IHL, 7.58 U d, IHL ,7.79(d,2H), 7.7(s,IH), 8.11(s, iH), 8.52(s,1I H), 9.02 (s,l H), 9.67 (s,1 H), 10.96 (s,1 H). 5 Example 10: Preparation of 4-(4-(3-chloro-4-fluorophenvlamino)quinazolin-6-yloxv) N-Hydroxybutanamide (Compound 15) The title compound 15 was prepared (20 mg) from compound 0209 from step 9e and ethyl 4-bromobutanoate using a procedure similar to that described for compound 13 (Example 9): mp128-132 0 C; LC-MS n/z 391 [M+1]; 'H 10 NMRDODMSO+D20 O62.05(m.2H),2.24-]t,2HO, 4.21(t,2H) 7.461Ot,1HE, 7.54(dd,1H) ,7.65 E1m,1HO, 7.76(d,1H) ,7.82(m 1H),7.99E m,1H 0,8.43(s,1H). Example 11: Preparation of 6-(4-(3-chloro-4-fluorophenylamino)quinazolin-6-loxv) N- hvdroxy hexanamide (Compound 17) 15 Step 1la. Ethyl 6-(4-(3-chloro-4-fluorophenylamino)quinazolin-6-yloxy)hexanoate (compound 0210-17) The title compound 0210-17 (0.2 g) was prepared from compound 0209 4-(3-chloro-4 fluorophenylamino) quinazolin-6-ol and ethyl 6-bromohexanoate using a procedure similar to that described for compound 0210-13 (Example 9): LC-MS n/z 433 [M+1],H 20 NMR1DDMSO O61.13(t,3H), 1.45(m,2H),1.60(m,2H) 1.76(m,2H),2.30(t, 2H), 4 .05(q, 2H) ,4.ll lt, 2H O, 7.41 Eld, 1H O,7.45(dd, 1H), 7.6811d, 1HLO, 7.80(m,1H),7.86(m,1H), 8.13(dd, 1H),8.48(s,1H). Step 1lb. 6

-(

4 -(3-Chloro-4-fluorophenylaminoq)cuinazolin-6-vloxy)-N hydroxyhexanamide (Compound 17) 25 The title compound 17 (30 mg) was prepared from compound 0210-17 using a procedure similar to that described for compound 13 (Example 9): LC-MS[M+1]419'H NMRDODMSOL61.28(m, ,2H), 1.60 (m,2H) 1.73(m,2H), 2.05 (t, 2H), 4.17 Ot, 2H0,7.25 Lid, IHE], 7.47(t, 1H), 7.55 (dd, IH) 7.76E1d, 1H0]7.73 Om,1H O,8.05(m,1IH),8.48(,s,1H). 30 EXAMPLE 12: Preparation of 7-( 4 -(3-chloro-4-fluorophenylamino)quinazolin-6 yloxy)-N- hydroxyheptanamide (Compound 18) 93 WO 2008/033749 PCT/US2007/077977 Step 12a. Ethyl 7-(4-(3-chloro-4-fluorophenylamino)quinazolin-6-yloxy)heptanoate (Compound 0210-18) The title compound 0210-18 (0.2 g) was prepared from compound 2-6 4-(3-chloro-4 fluorophenylamino)quinazolin-6-ol (0209) of step 9e and ethyl 7-bromoheptanoate using a 5 procedure similar to that described for compound 0210-13 (Example 9): LC-MS m/z 420 [M+1], 1 H-NMRO DMSOOS1.13(t, 3H), 1.36(m,2H),1.46(m,2H),1.54(m,2H) 1.78(m,2H),2.27(t, 2H), 4.05(q, 2H) ,4.11 Et, 2HiI, 7.41 Ed, IHE,7.47(dd, 1H), 7.70[0d, IHO, 7.81(m,1LH),7.84(m,1H), 8.13(dd, 1H),8.50(s,1H). Step 12b. 7-(4-(3-Chloro-4-fluorophenylamino)quinazolin-6-yloxy)-N 10 hydroxyheptanamide (Compound 18) The title compound 18 (20 mg) was prepared from compound ethyl 7-(4-(3-chloro-4 fluorophenylamino) quinazolin-6-yloxy-heptanoate (0210-18) using a procedure similar to that described for compound 13 (Example 9): LC-MS m/z 433 [M+1], mp145-149 OC H NMR FIDMSOLO61. 32(m, ,2H), 1.47 (m,4H) 1.88(m,2H), 1.94 (t, 2H), 4.120t, 15 2HL,7.43 [t, 1HEO, 7.51(dd,1H), 7.71 (d, 1H) 7.80]m, 1H[07.86L d, 1HEO,8.15(dd, 1H),8.51(s,IH). Example 13: Preparation of 2-(4-(3-ethynylphenylamino)quinazolin-6-vloxy) -N hvdroxyacetamide (Compound 19) 20 Step 13a. 4-(3-Ethynylphenylamino)quinazolin-6-yl acetate (Compound 0208) The title compound 0208 (0.8 g, yield: 73%) was prepared from 4-chloroquinazolin-6-yl acetate 0204 and 3-ethynylbenzenamine 0206 using a procedure similar to that described for compound 0207 (Example 9): LC-MS m/z 304 [M+1], H NMRHDMSO62.36F1s,3HnP,4.267s,1H F, 7.43Hd, 25 1HED,7.53(t,1H),7.770 d,1HEO,7.95(m,2H),8.02(d, 1H),8.71 Ds,1H ,8.96(s,1H). Step 13b. 4-(3-Ethynylphenylamino)quinazolin-6-ol (Compound 0211) The title compound 0211 (0.6 g, yield: 88%) was prepared using a procedure similar to that described for compound 0209 (Example 9): LC-MS m/z 262 [M+ 1], 1H NMRLIDMSOE64.170 s,1HEJ, 7.190 d,1HEO,7.36(t,1H),7.43 [dd,1H, 7.65(d,1H), 30 .82(d,1H), .950 d,1H],8.10(s,1H), .48(s,1H). Step 13c. Ethyl 2

-(

4 -(3-Ethynylphenylamino)quinazolin-6-yloxy)acetate (Compound 0212 19) 94 WO 20081033749 PCT/US2007/077977 The title compound 0212-19 (0.2 g, yield:75%) was prepared from 4-(3 ethynylphenylamino) quinazolin-6-ol 0211 and ethyl 2-bromoacetate using a procedure similar to that described for compound 0210-13 (Example 9): LC-MS m/z 322 [M+1], mpl81-182 0 CD 'H-NMRE DMSOE61 .28(t.3H), 5 4.20LDq,2H O,4.25(s,1H)4.32(s,2H),7.230 d,1H O,7.41(t,IH),7.570 dd,1Hi, 7.74(d,1H),7.91(d,1H), 7.950my,1H O, 8.10(s,1H), 8.48(s,1H). Step 13d. 2

-(

4

-(

3 -Ethynylphenylamino)quinazolin-6-yloxy)-N-hydroxyacetamide (Compound 19) The title compound 12 (40 mg) was prepared from ethyl 2-(4-(3-ethynylphenylamino) 10 uinazolin-6-yloxy) acetate 0212-19 using a procedure similar to that described for compound 13 (Example 9): LC-MS m/z 335 [M+1], mp:189-191 OC DIH NMRDODMSOE]64.27(s.1H),4.69E1s,2H1,7.39 Ed,1H), 7.49(t,1H), 7.76LOm, 2HL1, 7.83(m, 2H), 7.88(s,1H),8.10-]s,1H1D, 8.82(m, 1H). 15 EXAMPLE 14: Preparation of 4

-(

4 -(3-ethynylphenvlamino)cquinazolin-6-voxy)

-N

hydroxybutanamide (Compound 21) Step 14a. Ethyl 4

-(

4

-(

3 -ethynylphenylamino)quinazolin-6-yloxy)butanoate (Compound 0212-21) The title compound 0212-21 (0.2 g, 78%) was prepared from compound 4-(3 20 ethynylphenylamino)quinazolin-6-ol (0211) and ethyl 4-bromobutanoate using a procedure similar to that described for compound 0210-13 (Example 9): LC-MS m/z 376 [M+1], 'H NMR ODMSO I 61.12(t.3H),1.79(m,42H),2.32(t, 2H), 4.04 Dq,2HE ,4.16(t, 2H),4.21(s,1H), 7.02Ddd, 1H O,7.21(d, 1H),7.39[idd, 1HLO,7.70(t, 1H),7.88(s, 1H),8.OOEm, 1HF,8.51(s,1 H),8.65(s,1H). 25 Step 14b. 4

-(

4

-(

3 -Ethynylphenylamino)quinazolin-6-yloxy)-N-hydroxybutanamide (Compound 21) The title compound 21 (50 mg) was prepared from ethyl 4-(4-(3-ethynylphenylamino) quinazolin-6-yloxy)butanoate (0212-21) using a procedure similar to that described for compound 13 (Example 9): LC-MS m/z 363 [M+1], mp182-186 OCEH 30 NMR L DMSO O 62.02 (m,2H),2.20 (t,2H), 4.16(t,2H),4.20(s,1H), 7.24 [ d,1H I,7.43(t, lH),7.52E dd, 1HO,7.75(d. IH),7.94(m, 2H),8.06I s, 1H I,8.53(s,1H). 95 WO 20081033749 PCT/US2007/077977 EXAMPLE 15: Preparation of 6-(4-(3-ethynylphenylamino)quinazolin-6-vloxy) -N hydroxyhexanamide (Compound 23) Step 15a. 6-(4-(3-Ethynylphenylamino)quinazolin-6-yloxy) hexanoate (Compound 0212 23) 5 The title compound ethyl 6-(4-(3-ethynylphenylamino)quinazolin-6-yloxy) hexanoate (0212-23) (0.3 g, 64%) was prepared from compound 4-(3-ethynylphenylamino)quinazolin 6-ol (0211) and ethyl 6-bromohexanoate using a procedure similar to that described for compound 0210-13 (Example 9): LC-MS n/z 404 [M+1]. Step 15b. 6-(4-(3-Ethynylphenylamino)quinazolin-6-yloxy)-N-hydroxyhexanamide 10 (Compound 23) The title compound 23 (50 mg) was prepared from ethyl 6-(4-(3-ethynylphenylamino) quinazolin-6-yloxy)hexanoate (0212-23) using a procedure similar to that described for compound 13 (Example 9): LC-MS n/z 391 [M+1]Emp176-182 oCLO'H NMR ODMSO D 61.46(m,2H), 1.60(m,2H), 1.81(m,2H),2.00 (t,2H), 4.15(t,2H), 4.20(s,1H), 15 7.24Od, 1H 0,7.43 (t, 1H), 7.52]dd, 1HE, 7.72(d, 1H), 7.92(m, 2H), 8.041Os, 1H0],8.53(s,1H). EXAMPLE 16: 4-(4-(3-Chloro-4-fluorophenylamino)-7-methoxyquinazolin-6 yloxy)-N-hydroxybutanamide (Compound 4) 20 Step 16a. Ethyl 4-(4-(3-chloro-4-fluorophenylamino)-7-methoxyquinazolin-6-yloxy) butanoate (Compound 0110-4) The title compound 0110-4 was prepared as a yellow solid (600 mg, 88.4%) from compound 0109 from step I f (500 mg, 1.56 mmol) and methyl 5-bromopentanoate (320 mg, 1.64 mmol) using a procedure similar to that described for compound 0110-1 (Example 1): 25 LCMS: 434 [M+1]*; 1 H NMR (CDC 3 ):5 1.80-1.97 (m, 4H), 2.48 (t, J=6.6Hz, 2H), 3.67 (s, 3H), 3.97 (s, 3H), 4.18 (t, J= 7.2 Hz, 2H), 7.14 (t, J= 8.7 Hz, 1H), 7.24 (s, 1H), 7.29 (s, 1H), 7.66-7.11 (m, 1H), 7.96 (dd, J= 6.9 Hz, 2.7Hz, 1H), 8.03 (s, 1H), 8.66 (s, 1H). Step 16b. 4

-(

4 -(3-Chloro-4-fluorophenylamino)-7-methoxyquinazolin-6-yloxy)-N hydroxybutanamide (Compound 4) 30 The title compound 4 was prepared as a white solid (140 mg, 35%) form compound 0110 4 (400 mg, 0.92 mmol) using a procedure similar to that described for compound 1 (Example 1): LCMS: 435 [M+1 ]; 1 H NMR (DMSO-d 6 ): 6 1.69~1.84 (in, 4H), 2.07 (t, J 6.6 Hz, 2H), 3.94 (s, 3H), 4.15 (t, J= 6.0 Hz, 2H), 7.21 (s, 1H), 7.45 (t, J= 9.0 Hz, 1H), 96 WO 20081033749 PCT/US2007/077977 7.78~7.83 (in, 2H), 8.13 (dd, J=6.9 Hz, 2.4 Hz, 1H), 8.03 (s, 1H), 8.50 (s, 1H), 8.72 (s, 1H), 9.54 (s, IH), 10.41 (s, IH). EXAMPLE 17: 5-( 4 -(3-Ethynylphenylamino)-7-methoxyquinazolin-6-yoxy)-N 5 hydroxypentanamide (Compound 10) Step 17a. Methyl 5-( 4 -(3-ethynylphenylamino)-7-methoxyquinazolin-6-yloxy) pentanoate (Compound 0113-10) The title compound 0113-10 was prepared as a yellow solid (500 mg, 72%) from compound 0112 (500mg, 1.7 mmol) and methyl 5-bromopentanoate (211 mg, 0.89 minol) 10 using a procedure similar to that described for compound 0110-1 (Example 1): LCMS: 406 [M+1]. Step 17b. 5-(4-(3-Ethynylphenylamino)-7-methoxyquinazolin-6-yloxy)-N hydroxypentanamide (Compound 10) The title compound 10 was prepared as a white solid (200 mg, 40%) from compound 15 0113-10 (500 mg, 1.23 mmol) using a procedure similar to that described for compound 1 (Example 1): LCMS: 407 [M+1 ]; H NMR (DMSO-d): 6 1.71-1.85 (in, 4H), 2.07 (t, J =7.2 Hz, 2H), 3.93 (s, 3H), 4.16 (t, J=6.3 Hz, 2H), 4.20(s, 1H), 7.19 (in, 2H), 7.41 (t, J= 8.1 Hz, 1H), 7.84 (s, 1H), 7.90 (dd, J= 8.4 Hz, 1.2 Hz, 1H), 8.00 (t, J= 1.8 Hz, 1H), 8.50 (s, 1H), 8.72 (s, IH), 9.48 (s, 1H), 10.40 (s, 1H). 20 EXAMPLE 18: Preparation of 5-(4-(3-chloro-4-fluorophenylamino)quinazolin-6 yloxy)-N-hydroxypentanamide (Compound 16) Step 18a. ethyl 5-( 4

-(

3 -chloro- 4 -fluorophenylamino)quinazolin-6-yloxy)pentanoate (compound 0210-16) The title compound 0210-16 (0.2 g, 68%) was prepared from compound 0209 4-(3 25 chloro-4- fluorophenylamino) quinazolin-6-ol (0.2 g, 0.69 mmol) and methyl 5 bromopentanoate (0.14 g, 0.69 mmol) using a procedure similar to that described for compound 0210-13 (Example 9): LCMS 376 [M+1]*. Step 18b. 5-(4-(3-chloro- 4 -fluorophenylamino)quinazolin-6-yloxy)-N hydroxypentanamide (Compound 16 ) 30 The title compound 16 (24 mg, 67%) was prepared from compound 0210-16 (37 mg, 0.09 mmol) using a procedure similar to that described for compound 13 (Example 9): mp: 85.9 C; LCMS 405 [M+1]', 1 H NMR (DMSO-d 6 ) 6 1.74 (m, 4H), 2.04 (t, J= 7.5 Hz,2H), 97 WO 2008/033749 PCT/US2007/077977 4.14 (t, J= 6 Hz ,2H), 7.44 (t, J= 9 Hz,1H), 7.51(dd, J= 9 Hz, J= 2.4 Hz,1H), 7.73(d, J= 8.7 Hz,IH), 7.82 (m, 1H), 7.88 (d, J= 2.4,1H) 8.16 (dd, J= 6.9 Hz, J= 2.7 Hz 1H), 8.52(s, ,1 I), 8.69 (s, I f), 9.67 (s,1I H), 10.38 (s, 1H). 5 EXAMPLE 19: Preparation of 7

-(

4

-(

3 -ethynylphenylamino)quinazolin-6-yloxy)-N hydroxyheptanamide (Compound 24) Step 19a. Ethyl 7

-(

4

-(

3 -ethynylphenylamino)quinazolin-6-yloxy)heptanoate (Compound 0212-24) The title compound 0212-24 (0.21 g, 58%) was prepared from compound 4-(3 10 ethynylphenylamino)quinazolin-6-oI (0211) (0.23 g, 0.86 mmol) and ethyl 7 bromoheptanoate (0.20 g, 0.86 mmol) using a procedure similar to that described for compound 0210-13 (Example 9): LCMS 418 [M+1]. Step 19b. 7-(4-(3-Ethynylphenylamino)quinazolin-6-yloxy)-N-hydroxyheptanamide (Compound 24) 15 The title compound 24 (50 mg, 42%) was prepared from compound 0212-24 (123 mg, 0.29 mmol) using a procedure similar to that described for compound 13 (Example 9): LCMS 405 [M+l]-, 'H NMR(DMSO-d): 6 1.44 (m, 2H), 1.48 (in, 2H), 1.59 (in, 2H), 1.67 (m, 2H), 2.11 (t, J= 7.2 Hz, 2H), 3.50 (s, IH), 4.17 (t, J= 6.3 Hz, 2H), 7.28(d, J= 7.5 Hz, I H),7.37 (t, ,= 6.9 Hz, I H), 7.48 (d, J= 9.0 Hz, I H), 7.78 (dd, J= 21.3 Hz, J= 7.8 Hz, 20 1H), 7.93 (s, iH), 7.92 (in, 2H), 8.45(s, 1H). EXAMPLE 20: Example 1: Synthesis of 7

-(

4 -(3-Chloro-4-fluorophenylamino)-7- (2 methoxyethoxy) quinazolin-6-yIoxy)-N-hydroxyheptanamide (Compound 30) 25 Step 20a. Ethyl 3 -hydroxy-4-(2-methoxyethoxy) benzoate (Compound 0402-30) To a solution of 0401 (1.82 g, 10.0 mmol) in N, N-dimethylformamide (20 mL) was added potassium carbonate (1.38 g, 10.0 mmol). The mixture was stirred for 15 minutes and then a solution of 2-methoxyethyl 4 -methylbenzenesulfonate (2.30 g, 10.0 mmol) in N, N dimethylformamide (5 mL) was added slowly dropwise. The mixture was stirred 48 hours at 30 room temperature and filtered. The filtrate was concentrated in vacuo and the residue was dissolved in ethyl acetate (30 mL) then the organic layer was washed with brine (20 niL x3) and dried over sodium sulfate, filtered and evaporated to give the title product 0402-30 as a white solid (1.2 g, 50%): LCMS: 241 [M+1] -. 'H NMR (DMSO-d) S1.26 (t,. = 7.5 Hz, 98 WO 2008/033749 PCT/US2007/077977 3H), 3.65 (m, J= 1.5 Hz, 2H), 4.11 (m, J= 4.5 Hz, 2H), 4.21 (m, J= 4.5 Hz, 2H), 7.00 (d, J= 9 Hz, IH), 7.37 ( m, J= 2 Hz, 2H), 9.40 (s, IH). Step 20b. Ethyl 3

-(

7 -ethoxy-7-oxoheptyloxy)-4-(2-methoxyethoxy) benzoate (Compound 0403-30) 5 Compound 0402-30 (204.0 mg, 0.85 mmol) and ethyl 7-bromoheptanoate (201.0 mg, 0.85 mmol) and potassium carbonate (353.0 mg, 2.50 mmol) in N,N-dimethylformamide(5 mL) was stirred at 60 0 C for 3 hours. The mixture was filtrated. The filtrate was concentrated in vacuo and the residue was dissolved in ethyl acetate (30 mL) then the organic layer was washed with brine (20 mLx3) and dried over sodium sulfate, filtered and evaporated to give 10 the title product 0403-30 as a yellow solid (325 mg, 96%): LCMS: 397 [M+1] +. Step 20c. Ethyl 5-(7-ethoxy-7-oxoheptyloxy)-4-(2-methoxyethoxy)-2-nitrobenzoate (Compound 0404-30) Compound 0403-30 (325.0 mg, 0.82 mmol) was dissolved in acetic acid (2 mL) and stirred at room temperature. Then fuming nitric acid (0.39 g, 6.0 mmol) was added slowly 15 dropwise. The mixture was stirred at room temperature for 2 hours. Poured into ice-water (50 mL) and extracted with ethyl acetate (20 mLx2). The combined organic layer was washed with aqueous NaHCO 3 solution (10 mLx3) and brine (10 mLx3) and dried over sodium sulfate, filtered and evaporated to give the title product 0404-30 as a yellow oil (330 ng, 100%): LCMS: 442 [M+1J '. 20 Step 20d. Ethyl 2 -amino-5-( 7 -ethoxy-7-oxoheptyloxy)-4-(2-methoxyethoxy) benzoate (Compound 0405-30) A mixture of 0404-30 (370.0 mg 0.82 mmol), ethanol (4.4 mL), water (3 mL) and hydrogen chloride (0.08 mL) was stirred to form a clear solution. The powder iron (459.0 mg, 8.2 mmol) was added. The mixture was stirred at reflux for 30 minutes and cooled to 25 room temperature, adjust pH to 8 with 10% sodium hydroxide solution in ice-water bath. The mixture was filtered and the filtrate was concentrated to remove ethanol and was then extracted whit ethyl acetate (20 mLx2). The combined organic layer was washed with brine (10 mLx3) and dried over sodium sulfate, filtered and evaporated to give the title product 0405-30 as a yellow oil (315 mg, 93%): LCMS: 412 [M+1] +. 30 Step 20e. Ethyl 7

-(

7

-(

2 -methoxyethoxy)-4-oxo-3, 4 -dihydroquinazolin-6-yloxy) heptanoate (Compound 0406-30) A mixture of compound 0405-30 (315.0 mg, 0.76 mmol), ammonium formate (48.0 mg, 0.76 mmol) and formamide (2.46 mL) was stirred at 190 C for 3 hours. The reaction 99 WO 2008/033749 PCT/US2007/077977 mixture was cooled to room temperature. The formamide was removed under reduce pressure, and the residue was dissolved in ethyl acetate (30 mL). The organic layer was washed with brine (10 mLx5) and dried over sodium sulfate, filtered and evaporated to give the title product 0406-30 as a white solid (235 mg, 98%): LCMS: 393 [M+1] +. 5 Step 20f. Ethyl 7-(4-chloro-7-(2-methoxyethoxy) quinazolin-6-yloxy)heptanoate (Compound 0407-30) A mixture of product 0406-30 (235.0 mg, 0.6 mmol) and phosphoryl trichloride (3 mL) was stirred at reflux for 4 hours. When a clear solution was obtained, the excessive phosphoryl trichloride was removed under reduced pressure. The residue was dissolved in 10 ethyl acetate (30 mL) and the organic layer was washed in turn with water (10 mLx2), aqueous NaHCO 3 solution (10 mLx2) and brine (20 mLx 1), dried over sodium sulfate, filtered and evaporated to give the title product 0407-30 as a yellow solid (233 mg, 94%): LCMS: 411 [M+1]y. Step 20g. Ethyl 7

-(

4

-(

3 -chloro-4-fluorophenylamino)-7-(2-methoxyethoxy)-quinazolin-6 15 yloxy)heptanoate (Compound 0408-30) A mixture of product 0407-30 (117.0 m g, 0.28 mmol) and 3-chloro-4 fluorobenzenamine (50.0 mg, 0.34 mmol) in isopropanol (3 mL) was stirred at reflux overnight. The mixture was cooled to room temperature and resulting precipitate was isolated, washed with isopropanol and ether. The solid was then dried to give the title 20 compound 0408-30 as a yellow solid (102 mg, 70%): LCMS: 520 [M+1] *. Step 20h. 7-(4-(3-Chloro-4-fluorophenylamino)-7-(2-methoxyethoxy)quinazolin-6 yloxy)-N-hydroxyheptanamide (Compound 30) The freshly prepared hydroxylamine solution (3 mL, 2.0 mmol) was placed in 25 mL flask. Compound 408-30 (102.0 mg, 0.2mmol) was added and stirred at room temperature 25 for 24 hours. The mixture was neutralized with acetic acid / methanol. The mixture was concentrated under reduce pressure. The residue was purified by preparation HPLC to give the title compound 30 as a yellow solid (85 mg, 84%): LCMS: 507 [M+1]*; 1 H NMR (DMSO-d) 31.33(m, 2H), 1.50 (in, 4H), 1.79 (s, 2H), 1.94 (t, 2H), 3.29 (s, 3H), 3.72 (s, 2H), 4.11 ( s, 2H), 4.25 (s, 2H), 7.19 ( s, IH), 7.42 (t, 1H), 7.79 ( s, IH), 8.10 ( d, l1H), 8.47 30 (s, 1H), 8.65 ( s, 1H), 9.52 ( s, 1H), 10.33 (s, lH). 100 WO 2008/033749 PCT/US2007/077977 EXAMPLE 21: Preparation of 7-(4-(3-Ethynylphenylamino)-7-(2 methoxyethoxy)quinazolin-6-yloxy)-N-hydroxyheptanamide (Compound 36) Step 21a. Ethyl 7-( 4 -(3-ethynylphenylamino)-7-(2-methoxyethoxy)quinazolin-6 5 yloxy)heptanoate (Compound 0408-36) A mixture of product 0407-30 (102.0 mg, 0.25 mmol) and 3-ethynylbenzenamine (35.0 mg, 0.3 mmol) in isopropanol (3 mL) was stirred at reflux overnight. The mixture was cooled to room temperature and resulting precipitate was isolated, washed with isopropanol and ether. The solid was then dried to give the title compound 0408-36 as a yellow solid (88 10 mg, 72%): LCMS: 491 [M-1]f. Step 21b. 7-( 4 -(3-Ethynylphenylamino)-7-(2-methoxyethoxy)quinazolin-6-yloxy)-N hydroxyheptanamide (compound 36) The freshly prepared hydroxylamine solution (3 mL, 2 mmol) was placed in 25 ml flask. Compound 0408-36 (88.0 mg, 0. 18mmol) was added to this solution and stirred at room 15 temperature for 24 hours. The mixture was neutralized with acetic acid / methanol and was concentrated under reduce pressure. The residue was purified by preparative HPLC to give the title compound 36 as a white solid (40 mg, 47%): LCMS: 479 [M+1] '; 'H NMR (DMSO-d 6 ) t51.33( m, 2H), 1.50 (in, 4H), 1.79 (s, 1H), 1.94 (t, 2H), 3.72 (s, 21), 4.11 (s, 2H), 4.25 (s, 2H), 7.19 (s, 1H), 7.42 (t, 1H), 7.79 (s, 1H), 8.10 (d, 1H), 8.47 ( s, 1H), 8.65 20 ( s, 1H), 9.52 ( s, 1H), 10.33 ( s, 111). EXAMPLE 22: Preparation of N'-(4-(3-chloro-4-fluorophenylamino)-7 methoxyquinazolin-6-yI)-N 5 -hydroxyglutaramide (Compound 38) Step 22a. 7-Chloroquinazolin-4(3H)-one (Compound 0302) 25 A mixture of compound 0301(17.2 g, 100 mmol) and fornamide (20 mL) was stirred at 130 "C for 30 minutes and to 190 C for 4 hours. The mixture was allowed to cool to room temperature. It was then poured into a mixture of ice and water. The precipitate was isolated, washed with water and dried to give the title compound 0302 (15.8 g, 87.7%). H NMR (DMSO-d 6 ): 6 7.65 (dd, 1H), 7.72 (d, 1H), 8.12 (d, 1H), 8.36 (s, 1H). 30 Step 22b. 7-Chloro-6-nitroquinazolin-4(3H)-one (compound 0303) Compound 0302 (18.0 g, 100 mmol) was added portionwise to a stirred mixture of concentrated sulfuric acid (60 mL) and fuming nitric acid (60 mL) which had been cooled to 0 "C, the mixture was stirred at ambient temperature for 1 hour and then heated to 45 C 101 WO 20081033749 PCT/US2007/077977 overnight. The mixture was poured into the mixture of ice and water. The precipitate was isolated, washed with water and dried. Recrystallization from acetic acid to give the title compound 0303 (14.1 g, 62.7%). 'H NMR (DMSO-d): d 8.00 (s, 111), 8.27 (s, 1H), 8.65 (s, 1H), 12.70 (s, 1H). 5 Step 22c. 7-Methoxy-6-nitroquinazolin-4(3H)-one (compound 0304) A mixture of compound 0303 (4.0 g,18.0 mmol) and sodium (2.4 g, 45 mmol) in methanol (50 mL) was heated at 100 C in a sealed pressure vessel for 20 hours. The solution was neutralized with acetic acid and diluted with water to give the title compound 0304 (3.0 g, 77%). 'H NMR (DMSO-d): 84.10 (s, 3H), 7.40 (s, 1H), 8.24 (s, 1H), 8.50(s, 10 1H), 12.67 (s, 1H). Step 22d. 4-Chloro-7-methoxy-6-nitroquinazolinc (compound 0305) Compound 0304 (3.8 g, 17.2 mmol) was suspended in POC1 3 (75 mL), the mixture was heated to reflux for 4 hours. The additional POC1 3 was removed in a vacuum. The residue was dissolved in a mixture of dichloromethane (50 mL) and aqueous NaHCO 3 (50 mL) .The 15 organic layer was dried and the solvent was removed to give the title compound 0305 (3.4 g, 83%). 1 H NMR (DMSO-d 6 ): S 4.05 (s, 3H), 7.44 (s, 1 H), 8.27 (s, 1 H), 8.53 (s, 1H). Step 22e. N-( 3 -chloro-4-fluorophenyl)-7-methoxy-6-nitroquinazolin-4-amine Hydrochloride (compound 0307) A mixture of compound 0305 (3.4 g, 14,2 mmol) and 3-chloro-4-fluroaniline (0406) (2.2 20 g, 15.2 mmol) and isopropanol (120 mL) was stirred at reflux for 3 hours. The mixture was cooled to ambient temperature and the precipitate was isolated, washed with methanol and ether and then dried to give the title compound 0307 (4.66 g, 85%). 1H NMR (DMSO-d 6 ): .34.10 (s, 3H), 7.55 (dd, 2H), 7.74 (m, 1H), 8.07 (dd, 1H), 8.90 (s, 1H), 9.55 (s, 1H), 11.6 (s, 1 H). 25 Step 22f. N-( 3 -chloro-4-fluorophenyl)-7-methoxy-6-nitroquinazolin-4-amine (Compound 0308) A mixture of compound 0307 (3.5 g, 10.0 mmol) and iron dust (11.2 g, 200.0 mmol) and ethanol (100 mL) and concentrated hydrochloric acid (2 mL), and water (30 mL) was heated to reflux for 1 hour. Removed iron dust by filtration. The filtrate was concentrated to 30 1/5 volume. The precipitate was isolated and dried to give the title compound 0308 (2.2 g, 69%). 'H NMR (DMSO-d 6 ): 33.97 (s, 3H), 5.38 (s, 2H), 7.10 (s, 1H), 7.36 (s, 1H), 7.39 (t, tH), 7.80 (in, 1H), 8.08 (dd, 1H), 8.38 (s, 1H), 9.39 (s, IH). Step 22g. Methyl 3

-(

4 -(3-chloro-4-fluorophenylamino)-7-methoxyquinazolin-6 102 WO 2008/033749 PCT/US2007/077977 ylcarbamoyl)propanoate (Compound 0310-38) The compound 0308 (500.0 mg, 1.57 mmol) and triethylamine (165.0 mg, 1.65 mmol) was dissolved in dichloromethane (50 mL). The mixture was cooled to 0 "C and the solution of methyl 5-chloro-5-oxopentanoate (270 mg, 1.65 mmol) in dichloromethane (5 mL) was 5 added into above mixture dropwise under 0 "C in 20 minutes. The reaction mixture was allowed to stir at ambient temperature for 1 hour. The mixture was washed with water (50 mLx2) and brine (50 mL). The organic layer was dried over MgSO 4 , filtered and concentrated to give the title compound 0310-38 (550 mg, 78%), LCMS: 448 [M+1]. Step 22h. Ni-(4-(3-chloro-4-fluorophenylamino)-7-methoxyquinazolin-6-yl)-NS 10 hydroxyglutaramide (Compound 38) To a stirred solution of hydroxylamine hydrochloride (4.67 g, 67 mmol) in methanol (24 mL) at 0 'C was added a solution of potassium hydroxide (5.61 g, 100 mmol) in methanol (14 mL). After addition, the mixture was stirred for 30 minutes at 0 C and was allowed to stand at low temperature. The resulting precipitate was isolated, and the solution 15 was prepared to give free hydroxylamine. The above freshly prepared hydroxylamine solution (5.6 mL, 10.0 mmol) was placed in 10 mL flask. Compound 0310-38 (550.0 mg, 1.23 mmol) was added to this solution and stirred at 0 *C for 10 minutes and was allowed to warm to room temperature. The reaction process was monitored by TLC. The mixture was neutralized with acetic acid. The mixture 20 was concentrated under reduce pressure. The residue was purified by preparative HPLC to give the title compound 38 as a grey solid (250 mg, 45%): LCMS: 448 [M+1]; 'H NMR (DMSO-d 6 ): 81.85 (m, 2H), 2.06 (t,J= 7.5 Hz, 2H), 2.48 (t,J= 7.2 Hz, 2H), 4.00 (s, 3H), 7.24 (s, 1H), 7.42 (t, J= 9.0 Hz, 1H), 7.80 (m, 1H), 8.10 (dd, J= 7.2 Hz, 2.7 Hz, IH), 8.52 (s, 1H), 8.70 (s, 1H), 8.82(s, 1H), 9.48 (s, 1H). 9.79 (s, 1H), 10.40 (s, 1H). 25 EXAMPLE 23: Preparation of N'-(4-(3-chloro-4-fluorophenylamino)-7 methoxyquinazolin-6-yl)-A' 8 -hydroxyoctanediamide (Compound 40) Step 23a. Methyl 8-(4-(3-chloro-4-fluorophenylamino)-7-methoxyquinazolin-6 ylamino)-8-oxooctanoate (Compound 0310-40) 30 The title compound 0310-40 was prepared as a yellow solid (350 mg, 78%) from compound 0308 (319 mg, 1.0 mmol) and methyl 8-chloro-8-oxooctanoate (227 mg, 1.1 mmol) using a procedure similar to that described for compound 0310-38 (Example 22): LCMS: 489 [M+1-]. 103 WO 2008/033749 PCT/US2007/077977 Step 23b. N-(4-(3-chloro-4-fluorophenylamino)-7-methoxyquinazolin-6-yl)-NA hydroxyoctanediamide (Compound 40) The title compound 40 was prepared as a yellow solid (120 mg, 30%) from compound 0310-38 (400 mg, 0.8 mmol) using a procedure similar to that described for compound 38 5 (Example 22): LCMS: 490 [M+1]*; 1H NMR (DMSO-d): 81.29 (m, 4H), 1.48 (in, 2H), 1.59 (m, 2H), 1.93 (t, J= 7.2 Hz, 2H), 2.45 (t, J= 7.2 Hz, 2H), 4.00 (s, 3H), 4.18 (s, 1H), 7.26 (s, 1H), 7.41 (t, J= 9.0 Hz, 1H), 7.74 (m, 1H), 8.08 (d, J= 1.2 Hz, 1H), 8.54 (s, 1H), 8.66 (s, 1H1), 8.83 (s, 1H), 9.46 (s, 1H), 9.95 (s, 1H), 10.33 (s, 1H). 10 EXAMPLE 24: Preparation of N'-(4-(3-ethynylphenylamino)-7-methoxyquinazolin-6 yl)-N-hydroxyglutaramide (Compound 42) Step 24a. AT-(3-ethynylphenyl)-7-methoxy-6-nitroquinazolin-4-amine Hydrochloride (Compound 0307-42) The title compound 0307-42 was prepared as a yellow solid (4.7 g, 84.5%) from 15 compound 0305 (350 mg, 0.78 mmol) and 3-ethynylbenzenamine (2.34 g, 20.0 mmol) using a procedure similar to that described for compound 0306-38 (Example 22): LCMS: 321 [M+1]*; 'H NMR (DMSO-d): 84.11 (s, 3H), 4.24 (s, 1H), 7.42 (d, 1H), 7.50 (t, 1H), 7.61 (s, 1H), 7.79 (d, 1H), 7.93 (m, IH), 8.93 (s, 1H), 9.57 (s, 1H), 11.56 (bs, 1H). Step 24b. N-(3-ethynylphenyl)-7-methoxyquinazoline-4,6-diamine (Compound 0309-42) 20 The title compound 0309-42 was prepared as a yellow solid (2.0 g, 69%) from compound 0307-42 (3.2 g, 10.0 mmol) using a procedure similar to that described for compound 0308 38 (Example 22): LCMS: 291 [M+1]; 'H NMR (DMSO-d 6 ): 83.95 (s, 3H), 4.14 (s, IH), 5.33 (s, 2H), 7.08 (m, 2H), 7.34 (m, 2H), 7.88 (in, 1H), 8.04 (s, 1H), 8.36 (s, 1H), 9.29 (s, ItH). 25 Step 24c. Methyl 5-( 4

-(

3 -ethynylphenylamino)-7-methoxyquinazolin-6-ylamino)-5 oxopentanoate (Compound 0311-42) The title compound 0311-42 was prepared as a yellow solid (450 mg, 77%) from compound 0309-42 (407 mg, 1.4 mmol) and methyl 5-chloro-5-oxopentanoate (254 mg, 1.54 mmol) using a procedure similar to that described for compound 0310-38 (Example 30 22): LCMS: 419 [M+1]*. Step 24d. N'-(4-(3-ethynylphenylamino)-7-methoxyquinazolin-6-yl)-A hydroxyglutaramide (Compound 42) 104 WO 2008/033749 PCT/US2007/077977 The title compound 42 was prepared as a yellow solid (100 mg, 47%) from compound 0311-42 (211 mg, 0.5 mmol) using a procedure similar to that described for compound 38 (Example 22). 5 EXAMPLE 25: Preparation of M-(4-(3-ethynylphenylamino)-7-methoxyquinazolin-6 yI)-M-hydroxyadipamide (Compound 43) Step 25a. Methyl 6-(4-(3-ethynylphenylamino)-7-methoxyquinazolin-6-ylamino)-6 oxohexanoate (Compound 0311-43) The title compound 0311-43 was prepared as a yellow solid (530 mg, 71%) from 10 compound 0309-42 (500 mg, 1.72 mmol) and methyl 6-chloro-6-oxohexanoate (323 mg, 1.81 mmol) using a procedure similar to that described for compound 0311-42 (Example 24): LCMS: 433 [M+1]f. Step 25b. N'-(4-(3-ethynylphenylamino)-7-methoxyquinazolin-6-yl)- 6 hydroxyadipamide (Compound 43) 15 The title compound 43 was prepared as a yellow solid (105 mg, 24%) from compound 0311-43 (432 mg, 1.0 mmol) using a procedure similar to that described for compound 42 (Example 24): m.p.: 191.2~196.7 "C; LCMS: 434 [M+1],; 1 H NMR (DMSO-d): 8 1.58 (m, 4H), 1.98 (t, J= 6.3 Hz, 2H), 2.44 (m, 2H), 3.99 (s, 3H), 4.16 (s, 1H), 7.18 (d, J= 7.8 Hz, 1H), 7.25 (s, 1H), 7.37 (t, J= 8.1 Hz, 1H), 7.84 (d, J= 8.4 Hz, 1H), 7.98 (s, 1H), 8.51 (s, 20 1H), 8.66 (s, 1H), 8.82 (s, 1H), 9.42 (s, 1H), 9.73 (s, 1H), 10.35 (s, 1H). EXAMPLE 26: N-(4-(3-ethynylphenylamino)-7-methoxyquinazolin-6-y)hydroxyoctanediamide (Compound 44) Step 26a. Methyl 8-(4-(3-ethynylphenylamino)-7-methoxyquinazolin-6-ylamino)-8 25 oxooctanoate (Compound 0311-44) The title compound 0311-44 was prepared as a yellow solid (150 mg, 78%) from compound 0309-42 (120 mg, 0.4 mmol) and methyl 8-chloro-8-oxooctanoatc (91 mg, 0.44 mmol) using a procedure similar to that described for compound 0311-42 (Example 24): LCMS: 461 [M+1]*. 30 Step 26b. N'-(4-(3-ethynylphenylamino)-7-methoxyquinazolin-6-yl)-N hydroxyoctanediamide (Compound 44) The title compound 44 was prepared as a yellow solid (30 mg, 20%) from compound 0311-44 (150 mg, 0.3 mmol) using a procedure similar to that described for compound 42 105 WO 2008/033749 PCT/US2007/077977 (Example 24): LCMS: 462 [M+1] I 'H NMR (DMSO-d 6 ): 31.30 (in, 4H), 1.51 (in, 2H), 1.62 (m, 2H), 1.95 (t, J= 7.2 Hz, 2H), 2.45 (t, J= 7.2 Hz, 2H), 4.00 (s, 3H), 4.18 (s, 1H), 7.19 (d, J= 7.2 Hz, IH), 7.26 (s, 1H), 7.38 (t, J 7.8 Hz, 1H), 7.86 (d, J= 7.8 Hz, 1H), 7.99 (s, 1H), 8.52 (s, IH), 8.83 (s, 1H). 9.44 (s, 1H). 5 EXAMPLE 27: Preparation of (E)-3-(4-(2-(4-(3-chloro-4-fluorophenylamino)-7 methoxyquinazolin-6-yloxy) ethoxy)phenyl)-N-hydroxyacrylamide (Compound 66) Step 27a. (E)-Methyl 3-(4-hydroxyphenyl) acrylate (Compound 0501-66) A mixture of 4-hydroxycinnamic acid (8.2 g, 50 mmol) and a drop of H 2

SO

4 in methanol 10 (30 mL) was heated to reflux overnight. Then the solvent was evaporated, the residue was dissolved in ethyl acetate, washed with saturated NaHCO 3 solution twice, brine, dried over MgSO 4 , concentrated to give the title compound 0501-66 as white solid (8.7 g, 98%): LCMS: 179 [M+1]. Step 27b. (E)-Methyl 3-(4-(2-(tosyloxy)ethoxy)phenyl)acrylate (Compound 0502-66) 15 A mixture of compound 0501-66 (5.0 g, 28.0 mmol) and 2-bromoethanol (3.9 g, 62.0 mmol) and potassium carbonate in N, N- dimethylformamide was stirred at 80 0 C for 24 hours. The reaction process was monitored by TLC. The mixture was filtrated. The filtrate was concentrated under reduce pressure. The residue was wash with diethyl ether and dried to give (E)-methyl 3-(4-(2-hydroxyethoxy)phenyl)-acrylate as yellow solid (1.6 g, 26.0%): 20 LCMS: 223 [M+1] 4 . To a mixture of triethylamine (0.3 g, 3 mol) and dichloromethane (20 mL) was added tosyl chloride (285 mg, 1.5 mmol) batchwise and stirred for 0.5 hour. Compound (E) methyl 3 -(4-(2-hydroxyethoxy)phenyl)acrylate (333 mg, 1.5 mmol) was added into above mixture and heated to reflux for 24 hours. The reaction mixture was added saturated 25 ammonium chloride solution and the organic layer was separated and washed by brine, dried (MgSO 4 ), evaporated to give compound 0502-66 as white solid (200 mg, 36%): LCMS: 377 [M+1]*. Step 27c. (E)-Methyl 3-( 4

-(

2 -(4-(3-chloro-4-fluorophenylamino)-7-methoxyquinazolin-6 yloxy) ethoxy)phenyl) acrylate (Compound 0503-66) 30 A mixture of compound 0109 (176 mg, 0.55 mmol) and 0502-66 (152 mg, 0.94 mmol) and potassium carbonate in N, N- dimethylformamide was stirred at 80"C for 24 hours. The reaction process was monitored by TLC. The mixture was filtrated. The filtrate was 106 WO 20081033749 PCT/US2007/077977 concentrated under reduce pressure. The residue was wash with diethyl ether and dried to give the title compound 0503-66 as yellow solid (281 mg, 98%): LCMS: 524 [M+1] . Step 27d. (E)-3-(4-(2-(4-(3-Chloro-4-fluorophenylamino)-7-methoxyquinazolin-6 yloxy)ethoxy)phenyl)-N-hydroxyacrylamide (Compound 66) 5 The title compound 66 was prepared as a white solid (65 mg, 19%) from compound 0503 66 (346.0 mg, 0.66 mmol) using a procedure similar to that described for compound 1 (Example 1): LCMS: 525[M+1]'; 1 H NMR (DMSO-d 6 ): 6 3.93 (s, 3H), 4.48 (s, 4H), 6.31 (d,.J= 16.2 Hz, 1H), 7.05 (d,.J= 8.1 Hz, 2H), 7.21 (s, 1H), 7.44 (t,. = 9.0 Hz, 1H), 7.52 (d, J= 8.1 Hz, 2H), 7.78 (d, J= 10.2 Hz, 1H), 7.88 (in, 1H), 8.12 (dd, J= 6.6Hz, 2.7Hz, 1H), 10 8.50 (s, 1H), 8.96 (s, 1H), 8.50 (s, 1H), 9.56 (s, 1H), 10.65 (s, 1H). EXAMPLE 28: Preparation of N 1 -(4-(3-chloro-4-fluorophenylamino)-7- (2 methoxyethoxy) quinazolin-6-yi)- V-hydroxyglutaramide (Compound 68) 15 Step 28a. 7-(2-Methoxyethoxy)-6-nitroquinazolin-4(3H)-one (compound 0304-68) Sodium (2.07 g, 90 mmol) was added to 2-methoxyethanol (125 mL) at 0 C until sodium was dissolved. Compound 0303 (6.77 g, 30.0 mmol) was added to the solution. The mixture was stirred at 90 C for 24 hours and was then adjusted to pH7 by acetic acid. Water (50 mL) was added to the mixture and resulting yellow precipitate was isolated, washed with 20 water and dried to provide the title compound 0304-68 as a yellow solid (7.003 g, 88%): LCMS: 266 [M+1] +. Step 28b. 4-Chloro-7-(2-methoxyethoxy)-6-nitroquinazoline (compound 0305-68) A mixture of product 0304-68 (5.30 g, 20.0 mmol) and phosphoryl trichloride (50 mL) was stirred at reflux for 5 hours. When a clear solution was obtained, the excessive 25 phosphoryl trichloride was removed under reduced pressure. The residue was dissolved in ethyl acetate (100 mL) and the organic layer was washed in turn with water (30 mLx2), aqueous NaHCO 3 solution (20 mLx2) and brine (20 mLx 1), dried over sodium sulfate, filtered and evaporated to give the title product 0305-68 as a yellow solid (5.31 g, 94%): LCMS: 284 [M+1] *. 30 Step 28c. N-( 3 -chloro- 4 -fluorophenyl)-7-(2-methoxyethoxy)-6-nitroquinazolin-4-amine (compound 0306-68) A mixture of product 0305-68 (5.31 g, 18.7 mmol) and 3-chloro-4-fluorobenzenamine (5.45 g, 37.4 mmol) in isopropanol (150 mL) was stirred at reflux overnight. The mixture 107 WO 20081033749 PCT/US2007/077977 was cooled to room temperature and resulting precipitate was isolated, washed with methanol and ether. The solid was then dried to give the title compound 0306-68 as a yellow solid (5.70 g, 77%): LCMS: 393 [M11] '. Step 28d. N-(3-chloro-4-fluorophenyl)-7-(2-methoxyethoxy) quinazoline-4, 6-diamine 5 (compound 0308-68) A mixture of 0306-68 (5.70 g, 14.5 mmot), ethanol (165 mL), water (43.5 mL) and hydrogen chloride (2.9 mL) was stirred to form a clear solution. The powder iron (16.24 g, 290.0 mmol) was added. The mixture was stirred at reflux for 2 hours. Cooled to room temperature, adjusted pH to 11 with 10% sodium hydroxide solution in ice-water bath and 10 was filtered. The filtrate was concentrated to remove ethanol and extracted whit ethyl acetate (100 mLx2), The combined organic layer was washed with brine (30 mLx3) and dried over sodium sulfate, filtered and evaporated to give the title product 0308-68 as a yellow solid (4.92 g, 93%): LCMS: 363 [M+1] 4 . Step 28e. Methyl 5-( 4 -(3-chloro-4-fluorophenylamino)-7-(2-methoxyethoxy) 15 quinazolin-6- ylamino)-5-oxopentanoate (Compound 0310-68) The methyl 5-chloro-5-oxopentanoate (0.198 g, 1.2 mmol) was added to a solution of compound 0308-68 (0.22 g, 0.6 mmol) in 30 mL of dichloromethane and triethylamine (0.48 g, 4.8 mmol). The mixture was stirred for 2 hours at 0 0 C. The reaction mixture was then washed with water and dried over sodium sulfate, filtered and evaporated to give the 20 title product 0310-68 as a brown oil (270 mg, 92%): LCMS: 491 [M+1]. Step 28f. N'-( 4

-(

3 -chloro-4-fluorophenylamino)-7-(2-methoxyethoxy)quinazolin-6-yl)-N 5 hydroxyglutaramide (Compound 68) To a stirred solution of hydroxylamine hydrochloride (4.67 g, 67 mmol) in methanol (24 mL) at 0 C was added a solution of potassium hydroxide (5.61 g, 100 mmol) in 25 methanol (14 mL). After addition, the mixture was stirred for30 minutes at 0 C and was allowed to stand at low temperature. The resulting precipitate was isolated, and the solution was prepared to give free hydroxylamine. The above freshly prepared hydroxylamine solution (6 mL, 4.0 mmol) was placed in 25 mL flask. Compound 0310-68 (270 mg, 0.55mmol) was added to this solution and stirred at 30 room temperature for 4 hours. The mixture was neutralized with acetic acid / methanol. The mixture was concentrated under reduce pressure. The residue was purified by preparative HPLC to give the title compound 68 as a yellow solid (220 mg, 75%): LCMS: 492 [M+1]-; 'H NMR (DMSO-da): 1.83 (im, J= 7.5 Hz, 2H), 2.05 (t, J = 7.2 Hz, 2H), 2.43 (t, J= 6.9 108 WO 2008/033749 PCT/US2007/077977 Hz, 2H), 3.31 (s, 3H), 3.76 ( t, J= 4.5 Hz, 2H), 4.32 (t, J= 4.2 Hz, 2H), 7.28 (s, 1H), 7.40 (t, J= 9 Hz, IH), 7.77 (in, 1H), 8.10 (m, J= 2.1 Hz, 1H), 8.50 ( s, 1H), 8.67 ( s, IH), 8.752 ( s, I R), 9.33 ( s, 11H), 9.77 ( s, 111), 10.38 ( s, 11H). 5 EXAMPLE 29: Preparation of N 1 -(4-(3-chloro-4-fluorophenylamino)-7- (2 methoxyethoxy) quinazolin-6-yl)-1M- hydroxyadipamide (Compound 69) Step 29a. Methyl 6-(4-(3-chloro-4-fluorophenylamino)-7-(2-methoxyethoxy) quinazolin-6-ylamino)-6- oxohexanoate (Compound 0310-69) 10 The methyl 6-chloro-6-oxohexanoate (0.36 g, 1.76 mmol) was added to a solution of compound 0308-68 (0.15 g, 0.4 mmol), 25 mL of dichloromethane and triethylamine (0.162 g, 1.6 mmol). The reaction mixture was stirred for 2 hours at 0 C. The reaction was washed with water and dried over sodium sulfate, filtered and evaporated to give the title product 0310-69 as a brown oil (185 mg, 92%): LCMS: 505[M+1] +. 15 Step 29b. N 1 -(4-(3-chloro-4-fluorophenylamino)-7-(2-methoxyethoxy)quinazolin-6-yl)-N hydroxyadipamide (compound 69) The freshly prepared hydroxylamine solution (6 mL, 4mmol) was placed in 25 mL flask. Compound 0310-69 (185 mg, 0.3 8mmol) was added to this solution and stirred at room temperature for 4 hours. The mixture was neutralized with acetic acid / methanol. The 20 mixture was concentrated under reduce pressure. The residue was purified by preparative HPLC to give the title compound 69 as a white solid (150 mg, 74%): LCMS: 506[M+1]; 'H NMR (DMSO-d 4 ): 1.58( m, 4H), 1.98 (t, J= 5.7 Hz, 2H), 2.46 (t, 2H), 3.30 (s, 3H), 3.78 (t, J= 4.2 Hz, 2H), 4.32 (t, J= 5.1 Hz, 2H), 7.28 (s, IH), 7.39(t, J= 9 Hz, 1H), 7.79 ( m, 1I H), 8.11 ( m, J= 2.7 Hz, 1 H), 8.50 ( s, 1 H), 8.64 ( s, 1H), 8.75 ( s, 1 H), 9.25 ( s, 1H), 25 9.76 (s, 1H), 10.33 (s, 1H). 109 WO 2008/033749 PCT/US2007/077977 EXAMPLE 30: Preparation of N 1 -(4-(3-chloro-4-fluorophenylamino)-7- (2 methoxyethoxy) quinazolin- 6-yl)-M- hydroxyoctanediamide (Compound 70) Step 30a. Methyl 8-(4-(3-chloro-4-fluorophenylamino)-7-(2-methoxyethoxy) 5 quinazolin- 6-ylamino)-8- oxooctanoate (Compound 0310-70) Methyl 8-chloro-8-oxooctanoate (0.496 g, 2.4 mmol) was added to a solution of compound 0308-68 (0.219 g, 0.6 mmol), 30 mL of dichloromethane and triethylamine (0.48 g, 2.4 mmol). The mixture was stirred for 2 hours at 0 "C. The reaction was washed with water and dried over sodium sulfate, filtered and evaporated to give the title product 0310 10 70 as a brown oil (281 mg, 88%): LCMS: 533[M+1]. 1 H NMR (DMSO-d), 1.35( m, 4H), 1.58 (im, 2H), 1.61 (in, 2H), 2.29 (t, J= 7.2 Hz, 2H), 2.41 (t, J= 7.2 Hz, 2H), 3.35 ( s, 3H), 3.77 (t, J= 4.5 Hz, 2H), 4.32 (t, J= 4.5 Hz, 2H), 7.28 (s, 1H), 7.40 (t, J= 9.3 Hz, 1H), 7.78 (m, 1H), 8.11 (in, 1H), 8.50 ( s, 1H), 8.74 (s, 1H), 9.24 (s, 1H), 9.76 (s, 1H). Step 30b. N-(4-(3-chloro-4-fluorophenylamino)-7-(2-methoxyethoxy)quinazolin-6-yl)-N 15 hydroxyoctanediamide (compound 70) The freshly prepared hydroxylamine solution (6 mL, 4.0 mmol) was placed in 25 mL flask. Compound 0310-70 (281 mg, 0.53mmol) was added to this solution and stirred at room temperature for 4 hours. The mixture was neutralized with acetic acid / methanol. The mixture was concentrated under reduce pressure. The residue was purified by preparative 20 HPLC to give the title compound 70 as a yellow solid (126 mg, 40%): LCMS: 506[M+1]*; H NMR (DMSO-d6), l.35(m, 4H), 1.58 ( m, J= 6.9 Hz, 2H), 1.61 (m, J= 7.2 Hz, 2H), 1.93 (t, J= 7.2 Hz, 2H), 2.42 (t, J= 7.5 Hz, 2H), 3.35 ( s, 3H), 3.77 (t, J= 4.5 Hz, 2H), 4.32 (t, J= 4.5 Hz, 2H), 7.28 (s, lH), 7.40 ( t, J= 9.3 Hz, 1H), 7.78 (in, 1H), 8.11 (in, J= 2.4 Hz, 1 H), 8.50 ( s, 1H), 8.62 ( d, J= 1.5 Hz, 1H), 8.75 ( s, 1 H), 9.25 ( s, 1 H), 9.76 ( s, 25 1H), 10.31 ( s, 1H). EXAMPLE 31: Preparation of 7-(4-(3-ethynyl-4-fluorophenvlamino)-7 methoxyquinazolin-6-yloxy)-N-hydroxyheptanamide (Compound 75) Step 31a. 2-Bromo-1-fluoro-4-nitrobenzene (compound 0602) 30 To a solution of 1-bromo-2-fluorobenzene (35.0 g, 200 mmol) in 200 mL of concentrated sulfuric acid was added 20 mL of 68% nitric acid. The temperature of the mixture was maintained below 20 0 C. After the addition was completed, the mixture was stirred at 10 "C overnight, then diluted with ice water. The resulting solid was collected by 110 WO 2008/033749 PCT/US2007/077977 filtration. The solid was recrystallized from petroleum ether to give the title compound 0602 as a yellow solid (38 g, 89%): m.p.55.8-56.7 0 C, 'H NMR (DMSO-d 6 ): o 7.66 (t, J= 9 Hz, 11 -), 8.32 (m, 1 I), 8.58 (dd, J= 3 Hz, 6 Hz, 1 H). Step 31b. (( 2 -Fluoro-5-nitrophenyl) ethynyl) trimethylsilane (Compound 0603) 5 A mixture of compound 0602 (11.0g, 50 mmol) , ethynyltrimethylsilane (7.5 g, 75 mmol), triphenylphosphine (0.5 g) and palladium (II) acetate (0.25 g) in 125 mL of deaerated triethylamine was heated at 100 *C overnight under argon. The reaction was cooled and was filtrated, and the filtrate was concentrated to a dark brown oil which was distilled under reduce pressure to give title compound 0603 as a light brown solid (4.7 g, 10 40%). 'H NMR (CDCl 3 ): 5 0.3 (s, 9H, SiCH), 7.22 (t, J= 9.0 Hz, 1H), 8.2-8.5 (in, 2H). Step 31c. 4 -Fluoro-3-((trimethylsilyl) ethynyl) benzenamine (Compound 0604) In 25 mL of methanol was mixed with compound 0603 (3.5 g, 14.8 mmol) and iron filings (4.14g, 74.0 mmol). To this mixture was added concentrated hydrochrolic acid and water to adjust pH 4-5. The mixture was heated to reflux for 3 hours, cooled, and filtrated 15 through silica gel. The filtrate was concentrated to yield a yellow solid residue which was then extracted with ether. The combined organic phase was dried over magnesium sulfate and concentrated to give the title compound 0604 as a brown solid (2.69 g, 88%): LCMS 208 [M+1]*. Step 31d. 3-Ethynyl- 4 -fluorobenzenamine (Compound 0605) 20 Compound 0604 obtained above was treated with 100 mg potassium hydroxide in 20 mL of methanol at room temperature overnight. The solution was concentrated, dilute with water, brought to neutrality, and then extracted with ether. The combined organic phase was dried over magnesium sulfate, concentrated to yield the title compound 0605 as a brown oil (1.49 g, 85%): LCMS 136 [M+I ]'. The product was used in the next step without further 25 purification. Step 31e. 4-(3-Ethynyl-4-fluorophenylamino)-7-methoxyquinazolin-6-yI acetate (Compound 0606) A mixture of 4 -chloro-7-methoxyquinazolin-6-yl acetate (compound 0105) (252 mg, 1.0 mmol) and 3 -ethynyl-4-fluorobenzenamine (605) (200 mg, 1.5 mmol) in isopropanol (10 30 mL) was stirred and heated to reflux for 3 hours. The mixture was cooled to room temperature and resulting precipitate was isolated. The solid was then dried to give the title compound 0606 (260 mg, 74.0%) as a light yellow solid: LCMS: 352[M+l]-. Step 31 f. 4-(3-Ethynyl-4-fluorophenylamino)-7-methoxyquinazolin-6-ol (Compound 0607) 111 WO 2008/033749 PCT/US2007/077977 A mixture of compound 0606 (260 mg, 0.74 mmol), LiOH H 2 0 (250 mg, 5.8 mmol) in methanol (25 ml) and H 2 0 (25 ml) was stirred at room temperature for 0.5 hour. The mixture was neutralized by addition of dilution acetic acid. The precipitate was isolated and dried to give the title compound 0607 (234 mg, 100%) as a grey solid: LCMS: 310[M+1] t . 5 Step 31g. Ethyl 7-(4-(3-ethynyl-4-fluorophenylamino)-7-methoxyquinazolin-6 yloxy)heptanoate (Compound 0608-75) The title compound 0608-75 was prepared as a yellow solid (300 mg, 87.0%) from compound 607 (230 mg, 0.74 mmol) and ethyl 7-bromoheptanoate (176 mg, 0.74 mmol) using a procedure similar to that described for compound 0110-1 (Example 1): LCMS: 466 10 [M+I1]. Step 31h. 7-(4-(3-Ethynyl-4-fluorophenylamino)-7-methoxyquinazolin-6-yloxy)-N hydroxyheptanamide (compound 75) The title compound 75 was prepared as a white solid (176 mg, 70%) from compound 0608 (250 mg, 0.54 mmol) using a procedure similar to that described for compound 1 15 (Example 1): mp 150.4~164.5 *C (dec); LCMS: 453 [M+1]+, 'H NMR (DMSO-d): 1.33 (m, 2H), 1.48 (m, 4H), 1.80 (m, 2H), 1.94 (t, J= 7.2 Hz, 2H), 3.91 (s, 3H), 4.10 (t, J= 6.0 Hz, 2H), 4.51 (s, lH), 7.17 (s, 1H), 7.31 (t, J= 7.5 Hz, 1H), 7.77 (s, 1H), 7.85 (in, 1H), 7.98 (m, 1H), 8.45 (s, IH), 8.65 (s, 1H), 9.47 (s, IH), 10.33 (s, 1H). 20 EXAMPLE 32: Preparation of (R)-N-hydroxy-6-(7-methoxy-4-(1 phenylethylamino)quinazolin-6-yloxy)-hexanamide (Compound 77) Step 32a. (R)-7-Methoxy-4-(1-phenylethylamino)quinazolin-6-ol (Compound 0701-77) A mixture of compound 0105 (2.0 g, 8.0 mmol), (R)-1-phenylethanamine (2.91 g, 24.0 mmol) and isopropanol (50 mL) was stirred at 60 C overnight. lospropanol was 25 removed and the residue was purified by column chromatography to give the title compound 0701-77 (1.32 g, 56%). LCMS: 296 [M+1]. Step 32b. (R)-Ethyl 6-(7-methoxy-4-(1-phenylethylamino)quinazolin-6-yloxy)hexanoate (Compound 0702-77) A mixture of compound 0701-77 (500.0 mg, 1.69 mmol), K 2

CO

3 (700.0 mg, 5.07 30 mmol), ethyl 6-bromohexanoate (378.0 mg, 1.69 mmol) and DMF (20 mL) was heated at 60 *C for 3 h. The DMF was moved under reduced pressure, the residue was suspended in water, and the resulting solid was collected and dried to give the title compound 0702-77 (320 mg, 43%). LCMS: 438 [M+1]*. 112 WO 2008/033749 PCT/US2007/077977 Step 32c. (R)-N-Hydroxy-6-(7-methoxy-4-(1-phenylethylamino)quinazolin-6-yloxy) hexanamide (compound 77) A mixture of compound 0702-77 (320.0 mg, 0.73 mmol) and 1.77 mol/L NH 2 O-I/MeOH (4.0 mL, 6.77 mmol) was stirred at room temperature for 0.5 h. The reaction mixture was 5 neutralized with AcOH and concentrated. The residue was suspended in water and the resulting solid was isolated and dried to give crude product. This crude product was purified by pre-HPLC to give the title compound 77 (36 mg, 12%). LCMS: 425 [M+ I ;, H INMR (DMSO- d): 6 1.46 (m, 2H), 1.59 (in, 5H), 1.82 (m, 2H), 2.01 (t,. = 8.7 Hz, 2H), 3.90 (s, 3H), 4.10 (t, J= 6.3 Hz, 2H), 5.63 (in, 1H), 7.09 (s, 1H), 7.21 (m, IH), 7.32 (m, 2H), 7.42 10 (d, J= 7.2 Hz, 2H), 7.75 (s, 1H), 8.06 (d, J= 8.4 Hz, 1H), 8.27 (s, 1H), 8.67 (s, 1H), 10.36 (s, 1H). EXAMPLE 33: Preparation of (R)-N-hydroxy-6-(4-(1-phenylethylamino)-quinazolin 6-yloxy)hexanamide (Compound 78) 15 Step 33a. (R)-4-(1-Phenylethylamino)quinazolin-6-ol (Compound 0701-78) A mixture of compound 0204 (1.0 g, 4.5 mmol) and (R)-1-(3-chloro-4-fluoro phenyl)ethanamine (0.87 g, 5.0 mmol) in isopropanol (45mL) was stirred at 90 0 C for I hour. The mixture was cooled to room temperature and the resulting precipitate was isolated. The solid was washed in turn with isopropanol and methanol, dried to provide the 20 title compound (R)-4-(1-phenylethylamino)quinazolin-6-yl acetate as ayellow solid (0.62 g, 61%): LCMS 308 [M+1]*. A mixture of the above product (0.7 g, 2.3 mmol) and lithium hydroxide monohydrate (0.29 g, 6.81 mmol) in methanol (10 mL) / water (15 mL) was stirred at room temperature for I hour. The pH was adjusted to 4 with acetic acid and filtered. The collected 25 yellow solid was washed by water and dried to obtained title compound 0701-78 as a yellow solid (0.42 g, 62%), LCMS 266 [M+1]*. Step 33b. (R)-Ethyl 6-(4-(1 -phenylethylamino)quinazolin-6-yloxy)hexanoate (Compound 0702-78) A mixture of compound 0701-78 (0.31 g, 1.2 mmol), ethyl 6-bromohexanoate (0.27 30 g, 1.2 mmol) and K 2 C0 3 (0.8 g, 5.8 mmol) in DMF (15mL) was stirred and heated to 80 *C for 2 hours. The mixture was filtered and the filtrate was evaporated. The resulting solid was washed with ether to obtain the title compound 0702-78 as a pale yellow solid (0.2 g, 42.5%), LCMS 408 [M+1]f. 113 WO 20081033749 PCT/US2007/077977 Step 33c. (R)-N-Hydroxy-6-(4-(1-phenylethylamino)quinazolin-6-yloxy)hexanamide (Compound 78) The title compound 78 was prepared as a pale yellow solid (42 mg, 26%) from compound 0702-78 (168 mg, 0.41 mmol) using a procedure similar to that described for 5 compound 77 (Example 32): LCMS 395 [M+1]*, 'H NMR (DMSO- d 6 ): 6 1.47 (m, 2H), 1.52 (m, 2H) 1.65 (d,J= 7.2 Hz, 3H) 1.71 (m, 2H), 2.05 (t,J= 3.9 Hz, 2H), 4.04(t,J= 6.3 Hz, 2H), 5.56 (q, J= 6.3 Hz, 1H) 7.13 (t, J= 7.2 Hz, IH), 7.26 (t, J= 7.8 Hz, 2H), 7.32 (dd,.1 = 2.7, J= 9.0 Hz,1H) 7.39(d,.1 = 7.2 Hz, 2H[1, 7.56 (d, .= 7.2 Hz ,1H), 7.65 (m, 1H), 8.26 (s, 1HI). 10 EXAMPLE 34: Preparation of (R)-N-hydroxy-7-(7-methoxy-4-(1 phenylethylamino)quinazolin-6-yloxy)heptanamide (Compound 79) Step 34a. (R)-Ethyl 7-(7-methoxy-4-(1-phenylethylamino)quinazolin-6-yoxy) heptanoate (Compound 0702-79) 15 A mixture of compound 0701-79 (500 mg, 1.69 mmol), K 2 C0 3 (700 mg, 5.07 mmol), ethyl 7-bromoheptanoate (401 mg, 1.69 mmol) and DMF (20 mL) was heated at 60 C for 3 h. The DMF was removed under reduced pressure and the residue was suspended in water. The resulting solid was collected and dried to give the title compound 0702-79 (340 mg, 44%). LCMS: 452 [M+1]+. 20 Step 34b. (R)-N-Hydroxy-7-(7-methoxy-4-(1-phenylethylamino)quinazolin-6 yloxy)heptanamide (Compound 79) The title compound 79 was prepared (41 mg, 12%) from compound 0702-79 (340 mg, 0.75 mmol) using a procedure similar to that described for compound 77 (Example 32): LCMS: 439 [M+1]'; IH NMR (DMSO- d 6 ): 6 1.34 (in, 2H), 1.52 (n, 4H), 1.58 (d, J= 7.5 25 Hz, 2H), 1.80 (in, 2H), 1.99 (t, J= 8.7 Hz, 2H), 3.89 (s, 3H), 4.10 (t, J= 6.3 Hz, 2H), 5.62 (in, 1 H), 7.08 (s, 1H), 7.20 (in, 1H), 7.31 (in, 2H), 7.41 (d, J= 7.2 Hz, 2H), 7.74 (s, 1H), 8.05 (d, J= 8.1 Hz, lH), 8.26 (s, 1H), 8.63 (s, 1H), 10.32 (s, 1H). EXAMPLE 35: Preparation of (S)-N-hydroxy-7-(7-methoxy-4-(1 phenylethylamino)quinazolin-6-yloxy) heptanamide (Compound 80) 30 Step 35a. (S)-7-Methoxy-4-(1 -phenylethylamino) quinazolin-6-ol (Compound 0701-80) The title compound 0701-80 was prepared as a yellow solid (556 mg, 62.8%) from compound 0105 (750 mg, 3.0 mmol) and (S)-1-phenylethanamine (1089 mg, 9.0 mmol) 114 WO 2008/033749 PCT/US2007/077977 using a procedure similar to that described for compound 0701-77 (Example 32): LCMS: 296 [M1\+1]. Step 35b. (S)-Ethyl 7-(7-methoxy-4-(1-phenyl ethylamino) quinazolin-6-yloxy)heptanoate (Compound 0702-80) 5 The title compound 0702-80 was prepared as a yellow solid (160 mg, 70.95%) from compound 701-80 (148 mg, 0.5 mmol) and ethyl 7-bromoheptanoate (120 mg, 0.5 mmol) using a procedure similar to that described for compound 0702-77 (Example 32): L CMS: 452 [M+1]*. Step 35c. (S)-N-hydroxy-7-(7-methoxy-4-(1 -phenylethylamino) quinazolin-6 10 yloxy)heptanamide (Compound 80) The title compound 80 was prepared as a white solid (95 mg, 61.9%) from compound 0702-80 (160 mg, 0.35 mmol) and fresh NH 2

OH/CH

3 0H (3 mL, 5.3 Immol) using a procedure similar to that described for compound 77 (Example 32): m.p. 106.7-111.3*C, LCMS: 439 [M+1], 1 H NMR (DMSO- d): 6 1.42 (m, 6H), 1.57 (d, J= 6.6 Hz, 3H), 1.79 15 (in, 2H), 1.95 (t, J= 7.2 Hz, 2H), 3.88 (s, 3H), 4.08 (t, J= 6.9 Hz, 2H), 5.62 (, J= 6.6 Hz,1H), 7.06 (s, 1H), 7.21 (t, J= 7.5 Hz, 1H), 7.30 (t, J= 7.5 Hz, 2H), 7.41 (d, J= 7.5 Hz, 2H), 7.75 (s, 1H), 8.15 (d, J= 9.6 Hz, 1H), 8.29 (s, 1H), 8.60 (s, 1H), 10.30 (s, 1H). EXAMPLE 36: Preparation of (R)-7-(4-(1-(4-fluorophenyl) ethylamino)-7 methoxyquinazolin-6-yloxy)-N-hydroxyheptanamide (Compound 81) 20 Step 36a. (R)-4-(1-(4-Fluorophenyl) ethylamino)-7-methoxyquinazolin-6-o (Compound 0701-81) The title compound 0701-81 was prepared as a yellow solid (495 mg, 52.71%) from compound 0105 (750 mg, 3.0 mmol) and (R)-1-(4-fluorophenyl) ethanamine (1251 mg, 9.0 mmol) using a procedure similar to that described for compound 0701-77 (Example 32): 25 L CMS: 314 [M+1]'. Step 36b. (R)-Ethyl 7-(4-(1-(4-fluorophenyl) ethylamino)-7-methoxyquinazolin-6-yloxy) heptanoate (Compound 0702-81) The title compound 0702-81 was prepared as a yellow solid (190 mg, 81.0%) from compound 0701-81 (156 mg, 0.5 mmol) and ethyl 7-bromoheptanoate (120 mg, 0.5 mmol) 30 using a procedure similar to that described for compound 0702-77 (Example 32): LCMS: 470 [M+]-. 115 WO 2008/033749 PCT/US2007/077977 Step 36c. (R)-7-(4-(1-(4-Fluorophenyl) ethylamino)-7-methoxyquinazolin-6-yloxy)-N hydroxyheptanamide (Compound 81) The title compound 81 was prepared as a white solid (100 mg, 54.12%) from compound 0702-81 (190 mg, 0.40 mmol) and fresh NH 2

OH/CH

3 0H (3 mL, 5.3 1mmol) using a 5 procedure similar to that described for compound 77 (Example 32): m.p. 1 18.2-144.3 0 C, LCMS: 457 [M+1]+, 'H NMR (DMSO- d): 6 1.33 (m, 2H), 1.47 (in, 4H), 1.56 (d, J= 7.2 Hz, 3H), 1.78 (m, 2H), 1.95 (t, J= 7.2 Hz, 2H), 3.87 (s, 1H), 4.07 (t, J= 6.0 Hz, 2H), 5.60 (m, 1H), 7.06 (s, 1H), 7.11 (t,. = 9.0 Hz, 2H), 7.44 (m, 2H), 7.71 (s, 1H), 8.04 (d,. = 7.5 Hz, 1H), 8.25 (s, 1H), 8.65 (s, 1H), 10.33 (s, 1H). 10 EXAMPLE 37: Preparation of (R)-7-(4-(1-(4-chlorophenyl)ethylamino)-7 methoxyquinazolin-6-yloxy)-N-hydroxyheptanamide (Compound 82) Step 37a. (R)-4-(1-(4-Chlorophenyl)ethylamino)-7-methoxyquinazolin-6-ol (Compound 0701-82) 15 The title compound 0701-82 was prepared as a yellow solid (0.65 g, 49%) from compound 0105 (1.0 g, 4 mmol) and (R)-1-(4-chlorophenyl) ethanamine (1.87 g, 12 mmol) using a procedure similar to that described for compound 0701-77 (Example 32): LCMS: 300 [M+i]. Step 37b. (R)-Ethyl 7-(4-(1-( 4 -chlorophenyl)ethylamino)-7-methoxyquinazolin-6 20 yloxy)heptanoate (Compound 0702-82) The title compound 0702-82 was prepared as a yellow solid (460 mg, 56%) from compound 0701-82 (550 mg, 1.7 mmol) and ethyl 7-bromoheptanoate (404 mg, 1.7 mmol) using a procedure similar to that described for compound 0702-77 (Example 32): LCMS: 486 [M+1]'. 25 Step 37c. (R)-7-(4-(1-( 4 -Chlorophenyl)ethylamino)-7-methoxyquinazolin-6-yloxy)-N hydroxyheptanamide (Compound 82) The title compound 82 was prepared as a white solid (145 mg, 29%) from compound 0702-81 510 mg, 1.05 mmol) and fresh .77 mol/L NH 2 OH/MeOH (4.7 mL, 8.4 mmol) using a procedure similar to that described for compound 77 (Example 32): LCMS: 473 [M+1]+; 30 1H NMR (DMSO- d,): 3 1.34 (m, 2H), 1.47 (m, 4H), 1.57 (d, J= 6.9 Hz, 3H), 1.80 (m, 2H), 1.97 (t, J= 7.2 Hz, 2H), 3.89 (s, 3H), 4.10 (t, J= 6.6 Hz, 2H), 5.57 (m, 1H), 7.08 (s, 1H), 7.38 (d, J= 8.4 Hz, 2H), 7.43 (d, J= 8.4 Hz, 2H), 7.73 (s, 1H), 8.04 (d, J = 7.8 Hz, 1H), 8.23 (s, 1H), 8.64 (s,1H), 10.33 (s,1H). 116 WO 2008/033749 PCT/US2007/077977 EXAMPLE 38: Preparation of (R)-N-hydroxy-7-(7-methoxy-4-(1-(4 methoxyphenyl)ethylamino)quinazolin-6-yloxy)-heptanamide (Compound 83) 5 Step 38a. (R)-7-Methoxy-4-(1-(4-methoxyphenyl)ethylamino)quinazolin-6-ol (Compound 0701-83) A mixture of compound 0105 (1.0 g, 4.0 mmol), (R)-1-(4 methoxyphenyl)ethanamine (1.81 g, 12.0 mmol) and isopropanol (25 mL) was stirred at 60 C overnight. lospropanol was removed and the residue was purified by column 10 chromatogram to give the title compound 0701-83 (0.81 g, 62%). LCMS: 326 [M+1]. Step 38b. (R)-Ethyl 7-(7-methoxy-4-(1-(4-methoxyphenyl)ethylamino)quinazolin-6 yloxy)heptanoate (Compound 0702-83) A mixture of compound 0701-83 (630 mg, 1.94 mmol), K 2 C0 3 (804 mg, 5.8 mmol), ethyl 7-bromoheptanoate (459 mg, 1.94 mmol) and DMF (20 mL) was heated to 60 "C for 3 15 h. The DMF was moved away under reduced pressure, the residue was suspended in water, and the solid was collected and dried to give the title compound 0703-83 (440 mg, 47%). LCMS: 482 [M+1]*. Step 38c. (R)-N-Hydroxy-7-(7-methoxy-4-(i-(4-methoxyphenyl)ethylamino)-quinazolin-6 yloxy)-heptanamide (Compound 83) 20 A mixture of compound 0702-83 (530 mg, 1.1 mmol) and 1.77 mol/L

NH

2 OHI/MeOH (5 mL, 8.8 mmol) was stirred at room temperature for 0.5 h. The reaction mixture was neutralized with AcOH and then the mixture was concentrated and the residue was suspended in water, the precipitate was isolated and dried to give crude product. This product was purified by pre-HPLC to give the title compound 83 (151 mg, 29%). LCMS: 25 469 [M+1]; 'H NMR (DMSO- d 6 ): 5 1.32 (in, 2H), 1.45 (in, 4H), 1.54 (d, J= 6.9 Hz, 3H), 1.78 (in, 2H), 1.95 (t, J= 7.2 Hz, 2H), 3.69 (s,3H), 3.87 (s, 3H), 4.07 (t, J= 6.3 Hz, 2H), 5.56 (in, 1H), 6.87 (d, J= 8.7 Hz, 2H), 7.05(s, 1H), 7.31 (d, J= 8.7 Hz, 2H), 7.70 (s, 1H), 7.96 (d, J= 8.1 Hz, 1H), 8.26 (s, 1H), 8.62 (s,IH), 10.31 (s,1H). EXAMPLE 39: Preparation of 7-(4-(Benzylamino)-7-methoxyquinazolin-6-yoxy)-N 30 hydroxyheptanamide (Compound 85) Step 39a. 4 -(Benzylamino)-7-methoxyquinazolin-6-ol (0701-85) 117 WO 20081033749 PCT/US2007/077977 Benzylamine (1.28 g, 12.0 mmol) was added into a mixture of compound 0105 (1.0 g, 4.0 mmol) and 2-propanol (50 ml). The reaction mixture was then stirred at reflux for 3 hours. The mixture was cooled to room temperature and the resulting precipitate was isolated. The solid was then dried to give the title compound 0701-85 as a yellow solid (854 mg, 76%): 5 LCMS: 282 [M+1]. Step 39b. Ethyl 7-( 4 -(benzylamino)-7-methoxyquinazolin-6-yloxy)heptanoate (Compound 0702-85) The title compound 0702-85 was prepared as a yellow solid liquid (270 mg, 62%) from compound 0701-85 ( 2 81mg, 1.0 mmol) and ethyl 7-bromoheptanoate (236mg, 1 mmol) 10 using a procedure similar to that described for compound 0702-77 (Example 32): LCMS: 438 [M+1]. Step 39c. 7

-(

4 -(Benzylamino)-7-methoxyquinazolin-6-yloxy)-N-hydroxyheptanamide (compound 85) The title compound 85 was prepared as a yellow solid (64 mg, 24%) from compound 15 0702-85 (270 mg, 0.62 mmol) using a procedure similar to that described for compound 77 (Example 32): LCMS: 425[M+I]-; 1 H NMR (DMSO- dQ): o 1.32 (in, 2H), 1.42 (in, 2H), 1.51 (in, 2H), 1.76 (in, 2H), 1.94 (t, J= 7.2 Hz, 2H), 3.88 (s, 3H), 4.03 (t, J= 6.3 Hz, 2H), 4.76 (d, J= 5.4 Hz, 2H), 7.08 (s, 1H), 7.21 (t, J= 6.0 Hz, 2H), 7.30 (t, J= 6.0 Hz, 2H), 7.33 (t, 1= 6.6 Hz, 1 H), 7.63 (s, IH), 8.29 (s, 1H), 8.42 (t, J= 6.0 Hz, 1 H), 8.64 (s, I H), 10.32 (s, 20 1H). EXAMPLE 40: Preparation of 7-(4-(4-fluorobenzylamino)-7-methoxyquinazolin-6 yloxy)-N-hydroxyheptanamide (Compound 86) Step 40a. 4

-(

4 -Fluorobenzylamino)-7-methoxyquinazolin-6-ol (Compound 0701-86) 25 The title compound 0701-86 was prepared as a yellow solid (489 mg, 54.5%) from compound 0105 (750 mg, 3.0 mmol) and (4-fluorophenyl) methanamine (1125 mg, 9.0 mmol) using a procedure similar to that described for compound 0701-77 (Example 32): LCMS: 300[M+1]*. Step 40b. Ethyl 7

-(

4

-(

4 -fluorobenzylamino)-7-methoxyquinazolin-6-yloxy) heptanoate 30 (Compound 0702-86) The title compound 0702-86 was prepared as a yellow liquid (408 mg, 89.67%) from compound 0701-86 (300 mg, 1.0 mmol), ethyl 7-bromoheptanoate (237 mg, 1.0 mmol) 118 WO 2008/033749 PCT/US2007/077977 using a procedure similar to that described for compound 0702-77 (Example 32): LCMS: 456 [M+1]*. Step 40c. 7

-(

4

-(

4 -fluorobenzylamino)-7-methoxyquinazolin-6-yloxy)-N hydroxyheptanamide (Compound 86) 5 The title compound 86 was prepared as a white solid (300 mg, 69.97%) from compound 0702-86 (442 mg, 0.97 mmol) and fresh NH 2

OH/CH

3 0H (4 mL, 7.08mmol) using a procedure similar to that described for compound 77 (Example 32): LCMS: 443 [M+1] , H NMR (DMSO- d): 6 1.31~1.54 (m, 6H), 1.77 (m, 2H), 1.94 (t,. = 7.5 Hz, 2H), 3.88 (s, 3H), 4.03 (t, J= 6.3 Hz, 2H), 4.74 (d, J= 5.4 Hz, 2H), 7.11 (in, 3H), 7.38 (in, 2H), 7.68 (s, 10 1H), 8.30 (s, 1H), 8.40 (m, 1H), 8.60 (s, 1H), 10.30 (s, 1H). EXAMPLE 41: Preparation of 7-(4-(3, 4-difluorobenzylamino)-7- methoxyquinazolin 6 -yloxy)-N-hydroxyheptanamide (Compound 87) Step 41 a. 4-(3, 4 -Difluorobenzylamino)-7-methoxyquinazolin-6-ol (Compound 0701-87) The title compound 0701-87 was prepared as a light yellow solid (500 mg, 52.6%) from 15 compound 105 (750 mg, 3.0 mmol) and (3, 4-difluorophenyl) methanamine (1072 mg, 7.5 mmol) using a procedure similar to that described for compound 0701-77 (Example 32): LCMS: 318 [M+1]. Step 41b. Ethyl 7-(4-(3, 4-difluorobenzylamino)-7-methoxy-4a, 5-dihydroquinazolin-6 yloxy) heptanoate (Compound 0702-87) 20 The title compound 0702-87 was prepared as a light yellow solid (205 mg, 86.7%) from compound 0701-87 (160 mg, 0.5 mmol), ethyl 7-bromoheptanoate (237 mg, 1.0 mmol) using a procedure similar to that described for compound 0702-77 (Example 32): LCMS: 474 [M+1]]. Step 41c. 7

-(

4

-(

3

,

4 -difluorobenzylamino)-7-methoxyquinazolin-6-yloxy)-N 25 hydroxyheptanamide (Compound 87) The title compound 87 was prepared as a white solid (75 mg, 44.5%) from compound 0702-87 (173 mg, 0.366 mmol) and fresh NH 2

OH/CH

3 0H (2 mL, 3.4 mmol) using a procedure similar to that described for compound 77 (Example 32): LCMS: 461 [M+1]*, 1 H NMR (DMSO- d 6 ): 6 1.30 (in, 2H), 1.50 (m, 4H), 1.77 (m, 2H), 1.94 (t, J= 7.2 Hz, 2H), 30 3.88 (s, IH), 4.03 (t, J= 6.6 Hz, 2H), 4.72 (d, J= 6.0 Hz, 2H), 7.08 (s, 1H), 7.19 (s, 1H), 7.35 (m, 2H), 7.61 (s, IH), 8.30 (s, IH), 8.46 (t, J= 6.0 Hz, IH), 8.64 (s, IH), 10.32 (s, IH). 119 WO 2008/033749 PCT/US2007/077977 EXAMPLE 42: Preparation of 7-(4-(3-chloro-4-fluorobenzylamino)-7 methoxyquinazolin-6-yloxy)-N-hydroxyheptanamide (Compound 88) Step 42a. 4-(3-Chloro-4-fluorobenzylamino)-7-methoxyquinazolin-6-oI (Compound 0701 88) 5 The title compound 0701-88 was prepared as a light yellow solid (500 mg, 50.1%) from compound 0105 (750 mg, 3.0 mmol) and (3-chloro-4-fluorophenyl) methanamine (1435 mg, 9 mmol) using a procedure similar to that described for compound 0701-77 (Example 32): LCMS: 334 [M+1]'. Step 42b. Ethyl 7-(4-(3-chloro-4-fluorobenzylamino)-7-methoxyquinazolin-6 10 yloxy)heptanoate (Compound 0702-88) The title compound 0702-88 was prepared as a yellow solid (306 mg, 92.02%) from compound 0701-88 (227 mg, 0.68 mmol), ethyl 7-bromoheptanoate (161 mg, 0.68 mmol) using a procedure similar to that described for compound 0702-77 (Example 32): LCMS: 490 [M+1]>. 15 Step 42c. 7

-(

4

-(

3 -Chloro-4-fluorobenzylamino)-7-methoxyquinazolin-6-yloxy) hydroxyheptanamide (Compound 88) The title compound 88 was prepared as a white solid (210 mg, 70.02%) from compound 0702-88 (306 mg, 0.63 mmol) and fresh NH 2

OH/CH

3 0H (3 mL, 5.3 Immol) using a procedure similar to that described for compound 77 (Example 32): m.p.143.1 C (decomp.), 20 LCMS: 477 [M+1], 'H NMR (DMSO- d 6 ): 5 1.31 (m, 2H), 1.48 (m, 4H), 1.77 (i, 2H), 1.94 (t, J= 7.2 Hz, 2H), 3.89 (s, 3H), 4.04 (t, J= 6.6 Hz, 2H), 4.74 (d, J= 5.4 Hz, 2H), 7.09 (s, 1H), 7.35 (d, J= 7.8 Hz, 2H), 7.54 (d, J = 8.4 Hz, 1H), 7.63 (s, 1H), 8.35 (s, 1H), 8.58 (in, 1H), 8.65 (s, 1H), 10.33 (s, 1H), 11.92 (s, IH). EXAMPLE 43: Preparation of 7-(4-(3-bromobenzylamino)-7-methoxyquinazolin-6 25 yloxy)-N-hydroxyheptanamide (Compound 89) Step 43a. 4

-(

3 -Bromobenzylamino)-7-methoxyquinazolin-6-ol (Compound 0701-89) The title compound 0701-89 was prepared as a yellow solid (543 mg, 50.2%) from compound 0105 (750 mg, 3.0 mmol) and (3-bromophenyl) methanamine (1674 mg, 9 mmol) using a procedure similar to that described for compound 0701-77 (Example 32): 30 LCMS: 360 [M+i] 4 . Step 43b. Ethyl 7

-(

4

-(

3 -bromobenzylamino)-7-methoxyquinazolin-6-yloxy)heptanoate (Compound 0702-89) 120 WO 2008/033749 PCT/US2007/077977 The title compound 0702-89 was prepared as a yellow solid (230 mg, 89.15%) from compound 0701-89 (180 mg, 0.5 mmol), ethyl 7-bromoheptanoate (120 mg, 0.5 mmol) using a procedure similar to that described for compound 0702-77 (Example 32): LCMS: 516 [M+1]*. 5 Step 43c. 7-(4-(3-bromobenzylamino)-7-methoxyquinazolin-6-yloxy)-N hydroxyheptanamide (Compound 89) The title compound 89 was prepared as a white solid (105 mg, 53.96%) from compound 0702-89 (200 mg, 0.39 mmol) and fresh NH 2

OH/CH

3 0H (3 mL, 5.3 Immol) using a procedure similar to that described for compound 77 (Example 32): LCMS: 503 [M+1]P, 'H 10 NMR (DMSO- dQ): 6 1.31-1.56 (in, 6H), 1.75 (in, 2H), 1.94 (t, J= 7.2 Hz, 2H), 3.88 (s, 3H), 4.06 (t,,J= 6.6 Hz, 2H), 4.75 (d, J= 5.7 Hz, 2H), 7.08 (s, 1 H), 7.27 (t, J= 7.5 Hz, 1 H), 3.7.33 (in, 2H), 7.42 (s, 1H), 7.61 (s, 1H), 7.93 (s, 1H), 8.30 (s, 1H), 8.41 (t, J= 6.0 Hz, 1H), 8.60 (s, 1H), 10.29 (s, 1H). 15 EXAMPLE 44: Preparation of 4-(2-(4-(3-chloro-4-fluorophenylamino)-7 methoxyquinazolin-6-yloxy)ethoxy)-N-hydroxybenzamide (Compound 92) Step 44a. Methyl 4-(2-bromoethoxy)benzoate (Compound 0502-92) A mixture of compound 4-hydroxybenzoic acid methyl ester (457.0 mg, 3.0 mmol), 20 K 2 C0 3 (828 mg, 6 mmol) and 1,2-dibromoethane (10 mL) was heated at 130 C for 8 h. The 1,2-dibromoethane was removed under reduced pressure and the residue was suspended in water. The resulting precipitate was isolated and dried to give the title compound 0502-92 as a white solid (440 mg, 57%). LCMS: 259 [M+1] t . Step 44b. Methyl 4

-(

2 -(4-(3-chloro-4-fluorophenylamino)-7-methoxyquinazolin-6 25 yloxy)ethoxy)benzoate (Compound 0503-92) A mixture of compound 109 (384 mg, 1.2 mmol), K 2 C0 3 (276 mg, 2 mmol), compound 0502-92 (311 mg, 1.2 mmol) and DMF (10 mL) was heated at 40 C overnight. The DMF was removed under reduced pressure and the residue was suspended in water. The precipitate was collected and dried to give the title compound 0503-92 as a white solid (430 30 mg, 72%). LCMS: 259 [M+1]*. Step 44c. 4-(2-(4-(3-Chloro-4-fluorophenylamino)-7-methoxyquinazolin-6 yloxy)ethoxy)-A-hydroxybenzamide (Compound 92) 121 WO 2008/033749 PCT/US2007/077977 A mixture of compound 0502-92 (249 mg, 0.5 mmol) and 1.77 mol/L NH 2 OIMeOH (5 mL, 8.85 mmol) was stirred at room temperature for 0.5 h. The reaction mixture was neutralized with AcOH and the mixture was concentrated and the residue was suspended in water. The resulting precipitate was isolated and dried to give crude product. This crude 5 product was purified by pre-HPLC to give the title compound 92 as a white solid (80 mg, 32%). LCMS: 439 [M+1]; 1 H NMR (DMSO-d): 6 2.07 (s, 2H), 3.93 (s, 3H), 4.50 (s, 4H), 7.08 (d, J= 8.4 Hz, 2H), 7.22 (s, 2H), 7.44 (t, J= 9.0 Hz, 1H), 7.76 (m, 3H), 7.89 (s, 1H), 8.12 (m, 1H), 8.51 (s, IH), 8.87 (s, 1H), 9.54 (s, 1H), 11.05 (s, 1H). 10 EXAMPLE 45: Preparation of 7-(4-(3-ethynylphenylamino)-7-methoxyquinazolin-6 yloxy)-N-methoxyheptanamide (Compound 95) A mixture of compound 0802 (544 mg, 1.25 mmol) and Inodomethane (0804) (177 mg, 1.25 mmol) and potassium carbonate (1.0 g, 7.25 mmol) in N, N-dimethylformamide (15 mL) was stirred at room temperature for 12 hours. The solvent was removed under reduce 15 pressure and the residue was dissolved in ethyl acetate (50 mL). The organic layer was washed with saturation aqueous NaHCO 3 (20 mL) and brine (20 mL). The organic layer was dried over MgSO 4 and concentrated to give the title compound 95 as pale yellow solid (500 mg, 89%). m.p.195.8-197.0 'C; LCMS: 449 [M+1]-; 1 H NMR (DMSO-d 6 ); o 1.35 (m, 2H), 1.50 (m, 4H), 1.80 (in, 2H), 1.94 (t, J= 7.2 Hz, 2H), 3.54 (s, 3H), 3.92 (s, 3H), 4.12 (t, J= 20 6.3 Hz, 2H), 4.19 (s, 1H), 7.19 (in, 2H), 7.40 (t, J= 7.8 Hz, 1H), 7.80 (s, 1H), 7.87 (d, J= 9.6 Hz, 1H), 7.97 (s, 1H), 8.48 (s, 1H), 9.45 (s, 1H), 10.92 (s, IH). EXAMPLE 46: Preparation of N-acetoxy-7-(4-(3-chloro-4-fluorophenylamino)-7 methoxyquinazolin-6-yloxy)heptanamide (Compound 96) 25 A mixture of compound 0801 (50 mg, 0.108 mmol) and Ac 2 0 (204 mg, 2.0 mmol) and AcOH (2 mL) was stirred at room temperature for 1 h. The reaction mixture was neutralized with NaHCO 3 saturation solution. The precipitate was isolated and dried to give product 96 (42 mg, 77%). LCMS: 505 [M+1]*; 1 H NMR (DMSO- d): 6 1.40 (in, 2H), 1.50 (m, 2H), 1.55 (m, 2H), 1.80 (m, 2H), 2.09 (s, 3H), 2.12 (m, 2H), 3.94 (s, 3H), 4.13 (t,. = 30 6.9 Hz, 2H), 7.20 (s, 1H), 7.43 (t, J= 9.0 Hz, 1H), 7.78 (m, 1H), 7.84 (s, 1H), 8.12 (in, 1H4), 8.49 (s,1H), 9.67 (s,1H). EXAMPLE 47: Preparation of N-acetoxy-7-(4-(3-ethynylphenylamino)-7 122 WO 2008/033749 PCT/US2007/077977 methoxyquinazolin-6-yloxy)heptanamide (Compound 97) The title compound 97 was prepared as a solid (45 mg, 86.0%) from compound 0802 (48 mg, 0.11 mmol) and Ac 2 0 (204 mg, 2 mmol) using a procedure similar to that described for compound 96 (Example 46): LCMS: 476.5 [M+1]; 'H NMR (DMSO- dQ): c 1.40 (in, 5 2H), 1.46 (in, 2H), 1.58 (in, 2H), 1.80 (in, 2H), 2.12 (s, 3H), 2.13 (in, 2H), 3.94 (s, 3H), 4.14 (t, J= 6.6 Hz, 2H), 4.19 (s, 1H), 7.20 (d, J= 6.3 Hz, 2H), 7.40 (t, J= 7.8 Hz, 1H), 7.83 (s, 1H), 7.89 (d, J= 7.8 Hz, 11H), 7.99 (s, 1H), 8.49 (s,1H), 9.50 (s,1H), 11.55 (s, 1H). EXAMPLE 48: Preparation of N-(cyclohexanecarbonyloxy)-7-(4-(3 10 ethynylphenylamino)-7-methoxyquinazolin-6-yloxy)heptanamide (Compound 98) Compound 0802 (218 mg, 0.5 mmol) and triethylamine (75 ing, 0.75 mmol) were dissolved in acetone (20 mL) and N, N-dimethylformamide (2 mL). The reaction mixture was cooled to 0 C and a solution of cyclohexanecarbonyl chloride (73 mg, 0.5 mmol) in 15 acetone (5 mL) was added into the above solution dropwise. The reaction mixture was allowed to raise to ambient temperature and stirred for 1 hour. The mixture was concentrated under reduce pressure and the residue was purified by column chromatography to give the title compound 98 as a yellow solid (50 mg, 18%): LCMS: 545 [M+1]; 1H NMR (DMSO- d 6 ): 6 1.21~1.63 (n, 151H), 1.81 (in, 4H), 2.11 (t, J= 7.2 Hz, 2H), 3.92 (s, 20 3H), 4.12 (t, J = 7.2 Hz, 2H), 4.17 (s, 111), 7.19 (in, 2H), 7.39 (t, J= 7.8 Hz, 1H), 7.81 (s, 1H), 7.88 (d, J= 8.4 Hz, 111), 7.97 (s, 1H), 8.47 (s, 1H), 9.45 (s, 1H), 11.50 (s, 111). EXAMPLE 49: Preparation of 7-(4-(3-ethynylphenylamino)-7-methoxvquinazolin-6 yloxy)-N-(isobutyryloxy)heptanamide (Compound 99) 25 The title compound 99 was prepared as a yellow solid (100 mg, 44.0%) from compound 0802 (195 mg, 0.45 mmol) and isobutyryl chloride (48 mg, 0.45 mmol) using a procedure similar to that described for compound 98 (Example 48): LCMS: 505 [M+I]-; 'H NMR (DMSO- d): 6 1.10 (d, J= 7.2 Hz, 611), 1.39 (in, 2H), 1.47 (in, 2H), 1.56 (m, 2H), 1.81 (in, 2H), 2.11 (t,. = 7.5 Hz, 211), 2.68 (m, .= 7.2 Hz, 2H), 3.92 (s, 3H), 4.12 (t,./ = 6.6 Hz, 30 2H), 4.17 (s, 1H), 7.19 (m, 2H), 7.38 (t, J= 7.8 Hz, 1H), 7.82 (s, 1H), 7.88 (d, J= 8.7 Hz, 1H), 7.97 (s, 111), 8.47 (s, 111), 9.50 (s, 1H), 11.55 (s, 1H). 123 WO 2008/033749 PCT/US2007/077977 EXAMPLE 50: Preparation of 7-(4-(3-ethynylphenylamino)-7-methoxvquinazolin-6 yloxy)-N-(propionyloxv)heptanamide (Compound 100) The title compound 100 was prepared as a yellow solid (100 mg, 41.0%) from compound 0802 (218 mg, 0.5 mmol) and propionyl chloride (47 mg, 0.5 mmol) using a procedure 5 similar to that described for compound 98 (Example 48): LCMS: 491 [M+1]'; 'H NMR (DMSO- d): 6 1.05 (t, J= 7.5 Hz, 3H), 1.39 (in, 2H), 1.48 (m, 2H), 1.56 (m, 2H), 1.81 (m, 2H), 2.12 (t, J= 6.6 Hz, 2H), 2.41 (q, J= 7.5 Hz, 2H), 3.92 (s, 3H), 4.12 (t, J= 6.6 Hz, 2H), 4.18 (s, 1H), 7.19 (m, 2H), 7.38 (t,.1 = 7.8 Hz, IH), 7.80 (s, 1H), 7.88 (d, J= 8.1 Hz, 1H), 7.97 (s, IH), 8.47 (s, 1H), 9.45 (s, 1H), 11.53 (s, 1H). 10 EXAMPLE 51: Preparation of N-(benzoyloxy)-7-(4-(3-ethynylphenylamino)-7 methoxyquinazolin-6-yloxy)heptanamide (Compound 101) The title compound 101 was prepared as a yellow solid (150 mg, 56.0%) from compound 0802 (218 mg, 0.5 mmol) and benzoyl chloride (72 mg, 0.5 mmol) using a procedure 15 similar to that described for compound 98 (Example 48): LCMS: 539 [M+1]; 1H NMR (DMSO-d): 3 1.51 (m, 4H), 1.61 (m, 2H), 1.84 (m, 2H), 2.21 (t, J= 7.5 Hz, 2H), 3.93 (s, 3H), 4.14 (t, J= 6.9 Hz, 2H), 4.19 (s, 1H), 7.19 (m, 2H), 7.38 (t, J= 7.8 Hz, 1H), 7.55 (m, 2H), 7.72 (t, J= 7.8 Hz, IH), 7.82 (s, IH), 7.89 (d, J= 8.7 Hz, IH), 7.99 (m, 3H), 8.48 (s, I H), 9.48 (s, 1 H), 11.88 (s, 1 H). 20 EXAMPLE 52: Preparation of 7-(4-(3-ethynylbenzylamino)-7- methoxyquinazolin-6 yloxy)-N-hydroxyheptanamide (Compound 90) Step 52a. 4

-(

3 -Ethynylbenzylamino)-7-methoxyquinazolin-6-ol (Compound 0701-90) The title compound 701-90 was prepared as a light yellow solid (406 mg, 65%) from 25 compound 105 (520 mg, 2.06 mmol) and 3-ethynylbenzylamine (600 mg, 4.6 mmol) in isopropanol (20 mL) using a procedure similar to that described for compound 701-77 (Example 32): LCMS: 306 [M+1]*. Step 52b. Ethyl 7-(4-(3-ethynylbenzylamino)-7-methoxyquinazolin -6-yloxy) heptanoate (Compound 0702-90) 30 The title compound 0702-90 was prepared as a yellow solid (350 mg, 57%) from compound 0701-90 (406 mg, 1.33 mmol), potassium carbonate and ethyl 7 bromoheptanoate using a procedure similar to that described for compound 0702-77 (Example 32): LCMS: 462 [M+1]. 124 WO 2008/033749 PCT/US2007/077977 Step 52c. 7-(4-(3-ethynylbenzylamino)-7-methoxyquinazolin-6-yloxy) -N hydroxyheptanamide (Compound 90) The title compound 90 was prepared as a white solid (30 mg, 8.8%) from compound 0702-90 (350 mg, 0.76 mmol) and fresh NH 2

OH/CH

3 0H (2 mL, 3.54 mmol) using a 5 procedure similar to that described for compound 77 (Example 32): LCMS: 449 [M+1], 'H NMR (DMSO-d 6 ): 6 1.30-1.53 (m, 6H), 1.74-1.78 (in, 2H), 1.92-1.96 (m, 2H), 3.88 (s, 3H), 4.04 (t, J= 6.6 Hz, 2H), 4.11 (s, 1H), 4.75 (d, J= 4.5 Hz, 2H), 7.08 (s, 1H), 7.33-7.37 (m, 3H), 7.43 (s, 1H), 7.61 (s, 1H), 8.30 (s, IH), 8.41 (t,./ = 6.6 Hz, JH), 8.60 (s, 1H), 10.29 (s, lH). 10 EXAMPLE 53: Preparation of N-(6-(4-(3-chloro-4-fluorophenylamino)-7 methoxyquinazolin-6-yloxy)hexyl)-N-hydroxyacetamide (Compound 103) Step 53a. 6-(4-(3-Chloro-4-fluorophenylamino)-7-methoxyquinazolin 6-yloxy) hexan-1-ol (Compound 0901) 15 A mixture of compound 0109 (1.1 g, 3.44 mmol) and K 2

CO

3 (1.9 g, 13.76 mmol) in DMF (20 mL) was stirred at 40'C for 10 min. 6-Bromohexan-1-ol (0.64 g, 3.44 mmol) was added and the mixture was stirred at 60'C for 6 h. DMF was removed under reduced pressure and the residue was suspended in water. The resulting solid was collected and dried to give product 0901 (1.35 g, 93%). LC-MS: 420 [M+l]+. 20 Step 53b: N-acetoxyacetamide (Compound 0902-103) A mixture of hydroxylamine chloride (1.39 g, 20 mmol), sodium acetate (2.46 g, 30 mmol) and acetic anhydride (20.4 g, 200 mmol) in acetic acid (40 mL) was heated at reflux for 48h. The reaction mixture was filtrated and concentrated. The residue was added with water (20 mL) and extracted with ethyl acetate (30 mLx3). The organic layer was collected, 25 washed with saturated NaHCO 3 solution, brine, dried (MgSO 4 ), filtered and concentrated to give compound 0902-103 as a yellow liquid (2.11 g, 90%). Step 53c. N-Acetoxy-N-(6-(4-(3-chloro-4-fluorophenylamino)-7- methoxyquinazolin-6 yloxy)hexyl)acetamide (Compound 0903-103) A mixture of compound 0902-103 (117 mg, 1.0 mmol), compound 0901 (210 mg, 0.5 30 mmol) and PPh 3 (524 mg, 2.0 mmol) were dissolved in dry THF (50 mL). The reaction mixture was stirred at room temperature and was then added (E)-diisopropyl diazene- 1, 2 dicarboxylate (404 mg, 2.0 mmol) slowly. The mixture was heated to reflux for Ihour and concentrated. The residue (4.53 g) was purified by flash column chromatography on silica 125 WO 20081033749 PCT/US2007/077977 gel with petroleum ether: ethyl acetate=1:1 as eluant to give compound 0903-103 as a yellow solid (50 mg, 19%). Step 53d. N-(6-(4-(3-Chloro-4-fluorophenylamino)-7-methoxyquinazolin-6-yloxy)hexyl) N-hydroxyacetanide (Compound 103) 5 A mixture of compound 0903 (50 mg, 0.1 mmol) in methanol (2 mL) and water (2 mL) was added LiOH-H 2 0 (6 mg, 0.15 mmol). The reaction mixture was stirred at room temperature for 30 minutes and was neutralized by acetate acid. The mixture was evaporated to remove methanol. The resulting solid was filtrated, washed with water, diethyl ether to give the title compound 103 as an orange solid (32 mg, 70%). LCMS: 477 10 [M+1]4; 'H NMR (DMSO - d): 6 1.31 (in, 2H), 1.50 (m, 4H), 1.82 (m, 2H), 1.94 (s, 3H), 3.46 (t, J=7.2 Hz, 2H), 3.97 (s, 3H), 4.14 (t, J= 6.3 Hz, 2H), 7.28 (s, 1H), 7.54 (t, J=9.0 Hz, I H), 7.70 (m, 1 H), 8.03 (dd, 114), 8.16 (s, 1 H), 8.82(s, 114), 9.70 (s, 111). EXAMPLE 54: Preparation of N-(6-(4-(3-chloro-4-fluorophenylamino)-7 15 methoxyquinazolin-6-yloxy)hexyl)-N-hydroxypropionamide (Compound 106) Step 54a: N-(propionyloxy)propionamide (Compound 0902-106) Hydroxylamine chloride (1.39 g, 20 mmol) was dissolved in DMF (20 mL) and acetone (20 mL). The reaction was cooled to -10 C with ice/salt bath. To this cold solution was added Et 3 N (20 mL, 120 rnmol) and then propionyl chloride (7.4 g, 80 mmol) slowly. After 20 addition, the mixture was warmed to room temperature and stirred for lh. Water (50 mL) was added to the reaction mixture and extracted with ethyl acetate (100 mL x 3). The organic layer was collected, washed by saturated NaHCO 3 solution (20 mLx2) and brine (20 mL), dried (MgSO 4 ), filtered and concentrated to give the title product 0902-106 as an orange liquid (3.93 g, 100%): LCMS: 146 [M+1]+. 25 Step 54b: 7-( 4 -(3-Chloro-4-fluorophenylamino)-7-methoxyquinazolin-6-yloxy) -N-hydroxy-N-methylheptanamide (Compound 0903-106) To a mixture of compound 0902-106 (795 mg, 5.5 mmol), compound 0901-106 (419 mg, 1.0 mmol) and PPh 3 (1.31 g, 5.0 mmol) in dry THF (40 mL) was added (E)-diisopropyl diazene-1, 2-dicarboxylate (1.01 g, 5 mmol) slowly at room temperature. The mixture was 30 heated to reflux for lh and then concentrated to yield crude product 0903-106 (4.53 g) which was used to next step without further purifying. Step 54c: N-(6-(4-(3-Chloro-4-fluorophenylamino)-7-methoxyquinazolin-6-yloxy)hexyl) N-hydroxypropionamide (Compound 106) 126 WO 2008/033749 PCT/US2007/077977 To compound 0903-106 (4.53 g crude) was added NH3/EtOH solution (20 mL) in ice/water bath temperature. The reaction was then warmed to room temperature and stirred at room temperature over night. The reaction was filtered and the filtrate was concentrated to a residue which was purified by flash column chromatography on silica gel with ethyl 5 acetate/petroleum ether (1:1) as eluant to give the title compound 106 as a white solid (89 mg, two steps total yield 19%): m.p.149.2~158.0 C (dec); LCMS: 491 [M+1]; 'H NMR (DMSO - d): 6 0.92 (t,.1 = 7.5 Hz, 3H), 1.33 (in, 2H), 1.50 (in, 4H), 1.81 (in, 2H), 2.29 (in, 2H), 3.46 (t, J= 7.2 Hz, 2H), 3.90 (s, 3H), 4.09 (t, J= 5.4 Hz, 2H), 7.16 (s, IH), 7.41 (t, J 9.0 Hz, 111), 7.76 (s, 1H), 7.78 (s, 1H), 8.07 (dd, 1H), 8.46 (s, 1H), 9.51 (s, 1H), 9.53 (s, 10 1H). EXAMPLE 55: Preparation of (R)-N-hydroxy-5-(5-(7-methoxy-4 (1-phenylethylamino) quinazolin-6-vl)furan-2-yl)pent-4-ynamide (Compound 124) 15 Step 55a. (R)-6-iodo-7-methoxy-N-(1 -phenylethyl)quinazolin-4- amine (Compound 1001) A mixture of cone. H 2

SO

4 (7.1 g), acetonitrile (96 mL), acetic acid (96 mL) and water (96 mL) containing compound 0308 (3.0 g, 9.4 mmol) was cooled to 0 C and stirred for 0.5 h. The reaction mixture became a clear solution. To this solution was added NaNO 2 (0.72 20 g, 10.4 mmol) at 0 0 C. The resulting solution was stirred at room temperature for 0.5 hours and was then added dropwise to a solution of KI (4.68 g, 28 mmol) in water (96 mL) at 50 *C. After the addition was completed, the resulting solution was stirred at 50 C for another 0.5 hours. The reaction mixture was then cooled and filtered, washed with water and dried to give product 1001 as a yellow solid (2.5 g, 50% yield). 'H NMR (d 6 -DMSO) 6 10.12 (s, 25 IH), 9.16 (s, IH), 8.92 (s, 1H), 8.00 (dd, Ji, J 2 = 6.9 Hz, 2.4 Hz, 1H), 7.66-7.70 (in, 1H), 7.54 (t, J= 9.0 Hz, 1 H), 7.20 (s, IH). LC-MS: 406 (M+1). Step 55b. (R)-6-(Furan-2-yl)-7-methoxy-N-(1-phenylethyl)quinazolin-4-amine (Compound 1002) A mixture of 1001 (4.29 g, 10 mmol), 2-furanbomic acid (2.2 g, 20 mmol), Pd(OAc) 2 30 (224 mg, 1.0 mmol), PPh 3 (524 mg, 2.0 mmol), triethylamine (10 mL) and dimethylformamide (30 mL) was stirred at 80 C for 16 hours. The reaction mixture was cooled to room temperature and water (150 mL) was added. The resulting mixture was extracted with ethyl acetate (120 mLx4), dried and evaporated. The residue was purified by 127 WO 20081033749 PCT/US2007/077977 column chromatography (ethyl acetate: petroleum ether = 1: 3) to yield the product 1002 as a white solid (2.5 g, 67% yield). 1 H NMR (DMSO-d) 610.01 (s, 1 H), 8.83 (s, 1 H), 8.53 (s, 1H), 8.16-8.17 (in, 1H), 7.84-7.86 (m, 2H), 7.41 (t, J= 8.1 Hz, 1H), 7.29 (s, 1H), 7.06 (s, lH), 6.66 (s, 1H), 4.05 (s, 3H). LC-MS: 370 (M+1). 5 Step 55c. (R)-6-(Furan-2-yl)-7-methoxy-N-(1-phenylethyl)quinazolin-4-amine (Compound 1003) To a solution of 1002 (1.48 g, 4 mmol) in trifuroacetic acid (2 mL) and acetonitrile (40 mL) was added NIS (650 mg, 5 mmol). The solution was stirred at room temperature for 10 min. The mixture was neutralized with aqueous Na 2

CO

3 and concentrated. The resulting 10 mixture was extracted with ethyl acetate, washed with water, dried, and concentrated to give a residue which was purified by column chromatography to afford 1003 as a yellow solid (1.1 g, 58 % yield). 1 H NMR (DMSO-d 6 ) 6 10.08 (s, IH), 8.72 (s, IH), 8.55 (s, 1H), 8.15 (dd, J 1 , J 2 = 6.9 Hz, 2.7 Hz, 1H), 7.79-7.83 (in, 1H), 7.45 (t, J = 9.0 Hz, 1H), 7.31 (s, 1H), 7.00 (d, J = 3.6 Hz, 1H), 6.91 (d, J = 3.6 Hz, 1H), 4.06 (s, 3H) . LC-MS: 496 (M+1). 15 Step 55d. (R)-Methyl 5-(5-(7-methoxy-4-(1-phenylethylamino)quinazolin-6-yl) furan-2-yl) ynoate (Compound 1004-124) A mixture of 1003 (250 mg, 0.5 mmol), methyl pent-4-ynoate (112 mg, 1.0 mmol), Pd(OAc) 2 (35 mg, 0.05 mmol), PPh 3 (13 mg, 0.05 mmol), Cul (10 mg, 0.05 mmol), Et 3 N (0.5 mL) and DMF (3 mL) was stirred at 40 0 C under nitrogen for 16h. The mixture was 20 then diluted with water (120 mL) and extracted with ethyl acetate (100 mLx4). The combined organic layer was concentrated and purified by column chromatography (ethyl acetate: petroleum ether = 1:4) to afford 1004-124 as a yellow solid (180 mg, 78 % yield). LC-MS: 480 (M+1). Step 55e. (R)-N-Hydroxy-5-(5-(7-methoxy-4-(1-phenylethylamino)quinazolin-6-yl) 25 furan-2-yl)pcnt-4-ynamide (Compound 124) To a flask containing compound 1004-124 (180 mg, 0.37 mmol) was added a solution of hydroxylamine in methanol (3.0 mL). The mixture was stirred at room temperature for 0.5h. Then it was adjusted to PH 7 using acetic acid. The mixture was filtered, washed with methanol to afford the product 124 as a white solid (100 mg, 55 % yield). 1 H NMR 30 (DMSO-d 6 ) 610.52 (s, 1 H), 10.13 (s, 1 H), 8.85 (s, 1 H), 8.79 (s, 1 H), 8.53 (s, 1 H), 8.12-8.16 (m, 111), 7.79-7.83 (m, 1H), 7.43 (t, J = 9.6 Hz, IH), 7.30(s, 111), 7.09 (d, J = 3.6 Hz, 1H), 6.85 (d, J = 3.6 Hz, 1H), 4.05 (s, 3H), 2.73 (t, J = 7.2 Hz, 2H), 2.26 (t, J= 7.2 Hz, 2H). LC MS: 481 (M+I1). 128 WO 2008/033749 PCT/US2007/077977 EXAMPLE 56: Preparation of (R)-N-hydroxy-6-(5-(7-methoxy-4-(1 phenylethylamino)quinazolin-6-yl)furan-2-yl)hex-5-ynamide (Compound 125): 5 Step 56a. (R)-Methyl 6-(5-(7-methoxy-4-(1-phenylethylamino) quinazolin-6-yl) furan-2-yl)hex-5-ynoate (Compound 1004-125) The title compound 1004-125 was prepared as a yellow solid (180 mg, 77%) from compound 1003 (250 mg, 0.5 mmol) and methyl hex-5-ynoate (126 mg, 1.0 mmol) using a procedure similar to that described for compound 1004-124 (Example 55): LCMS: 494 10 [M+1]*. Step 56b. (R)-N-Hydroxy-6-(5-(7-methoxy-4-(1-phenylethylamino)quinazolin-6-yl) furan-2-yl) hex-5-ynamide (Compound 125) The title compound 125 was prepared as a white solid (60 mg, 13%) from compound 1004-125 (160 mg, 0.34 mmol) and hydroxylamine in methanol (3.0 mL) using a procedure 15 similar to that described for compound 124 (Example 55): 'H NMR (DMSO-d) p10.43 (s, tH), 10.11 (s, 1H), 8.79 (s, 1H), 8.73 (s, IH), 8.53 (s, 1H), 8.12-8.15 (in, 1H), 7.77-7.82 (in, 1 H), 7.43 (t, J = 9.0 Hz, I H), 7.30(s, 1 H), 7.09 (d, J = 3.6 Hz, 1 H), 6.86 (d, J= 3.6 Hz, I H), 4.05 (s, 3H), 2.52 (t, J = 6.6 Hz, 2H), 2.10 (t, J = 7.2 Hz, 2H), 1.72-1.82 (in, 2H). LC-MS: 495 (M+1). 20 EXAMPLE 57: Preparation of methyl 5-(4-(3-chloro-4-fluorophenylamino)-7 metho xyquinazolin-6-yl)pent-4-ynoate (Compound 138) Step 57a. Methyl 5-( 4 -(3-chloro-4-fluorophenylamino)-7-metho xyquinazolin-6-yl) pent-4-ynoate (Compound 1101-138) 25 A mixture of 1001 (215 mg, 0.5 mmol), methyl pent-4-ynoate (224 mg, 2.0 mmol), Pd(OAc) 2 (140 mg, 0.2 mmol), PPh (52 mg, 0.2 mmol), Cul (76 mg, 0.4 mmol), Et 3 N (2.5 mL) and DMF (5 mL) was stirred at 80 *C for 16h. Water (120 mL) was added to the reaction and the resulting mixture was extracted with ethyl acetate. The organic phase was combined, dried, filtered and concentrated to leave a residue which was purified by column 30 chromatography (ethyl acetate: petroleum ether = 1:4) to afford 1101 as a yellow solid (160 g, 77 % yield). LC-MS: 414 (M+1). Step 57b. Methyl 5-(4-(3-chloro-4-fluorophenylamino)-7-metho xyquinazolin-6-yl) pent-4-ynoate (Compound 138) 129 WO 20081033749 PCT/US2007/077977 To a flask containing compound 1101-138 (102 mg, 0.25 mmol) was added freshly prepared solution of hydroxylamine in methanol (3.0 mL). The mixture was stirred at room temperature for 0.5h. It was then adjusted to PH 7 using acetic acid. The resulting precipitate was filtered and washed with methanol to afford the product 138 as a white solid 5 (75 mg, 74 % yield). 'H NMR (DMSO-d 6 ) 6 10.49 (s, 1H), 9.81 (s, 1H), 8.81 (s, 1H), 8.56 (s, 1H), 8.55 (s, IH), 8.17-8.20 (in, 1H), 7.79-7.84 (in, 1H), 7.42 (t, J = 9.0Hz, 1H), 7.19 (s, 1H-), 3.94 (s, 3H), 2.72 (t, J = 7.2 Hz, 2H), 2.28 (t, J = 7.2 Hz, 2H). LC-MS: 415 (M+1). EXAMPLE 58: Preparation 6-(4-(3-chloro-4-fluorophenylamino)-7 10 methoxyquinazolin-6-yl)-N-hydroxyhex-5-ynamide (Compound 139) Step 58a. Methyl 6-(4-(3-chloro-4-fluorophenylamino)-7-methoxyquinazolin 6-yl)hex-5-ynoate (Compound 1101-139) The title compound 1101-139 was prepared as a yellow solid (890 mg, 53 % yield) from compound 1001 (1.7 g, 3.96 mmol) and methyl hex-5-ynoate (378 mg, 3.0 mmol) 15 using a procedure similar to that described for compound 1101-138 (Example 57): LCMS: 428 [M+1]. Step 58b. 6-(4-(3-Chloro-4-fluorophenylamino)-7-methoxyquinazolin-6-yl)-N hydroxyhex-5-ynamide (Compound 139) The title compound 139 was prepared as a white solid (80 mg, 73 %) from 20 compound 1101-139 (110 mg, 0.26 mmol) and freshly prepared hydroxylamine in methanol (3.0 mL) using a procedure similar to that described for compound 138 (Example 57): 'H NMR (DMSO-d 6 ) 6 10.42 (s, 1H), 9.91 (s, 1H), 8.70 (s, 1H), 8.60 (s, 1H), 8.58 (s, 1H), 8.16-8.19 (m, 1H), 7.78-7.85 (m, lH), 7.43 (t, J = 9.0Hz, 1H), 7.20 (s, 1H), 3.95 (s, 3H), 2.51 (t, J = 7.2 Hz, 2H), 2.15 (t, J = 7.2 Hz, 2H), 1.75-1.84 (in, 2H). LC-MS: 429 (M+1). 25 EXAMPLE 59: Preparation of 5-(4-(3-chloro-4-fluorophenylamino)-7 methoxyquinazolin-6-yl)-N-hydroxypentanamide (compound 144) Step 59a. Methyl 6-(4-(3-chloro-4-fluorophenylamino)-7-methoxyquinazolin 6-yl)hex-5-ynoate (Compound 1102-144) 30 To a solution of 1101-138 (500 mg, 0.21 mmol) in methanol (30 nL) was added 50 mg of Pd/C (10%). The mixture was stirred at room temperature under hydrogen atmosphere (1 atm) for 16 h. The mixture was filtered, and the filtrate was concentrated to give the crude 130 WO 20081033749 PCT/US2007/077977 1102-144 (480 mg, 94% yield) which was used directly in the next step. LC-MS: 418 (M+1). Step 59b. 5-(4-(3-Chloro-4-fluorophenylamino)-7-methoxyquinazolin-6-yl)-N 5 hydroxypentanamide (Compound 144) To a flask containing compound 1102-144 (480 mg, 1.14 mmol) was added a solution of freshly prepared hydroxylamine in methanol (5.0 mL). The mixture was stirred at room temperature for 0.5h. It was then convert to PH 7 using acetic acid. The resulting solid was filtered, washed with methanol to yield the product 144 as a white solid (400 mg, 83 % 10 yield). 'H NMR (DMSO-d 6 ) 8 10.34 (s, 1H), 9.69 (s, 1H), 8.68 (s, 1H), 8.53 (s, 1H), 8.24 (s, 1H), 8.19-8.23 (m, 1H), 7.80-7.88 (in, IH), 7.41 (t, J= 9.0Hz, 1H), 7.17 (s, 1H), 3.94 (s, 3H), 2.71 (t, J = 6.6 Hz, 2H), 2.00 (t, J= 7.2 Hz, 2H), 1.58-1.60 (m, 4H), 1.26-1.36 (m, 2H). LC-MS: 419 (M+I1). 15 EXAMPLE 60: Preparation of 5-(4-(3-chloro-4-fluorophenylamino)-7 methoxyquinazolin-6-yl)-N-hydroxypentanamide (Compound 145) Step 60a. Methyl 6-(4-(3-chloro-4-fluorophenylamino)-7-methoxyquinazolin 6-yl)hex-5-ynoate (Compound 1102-145) The title compound 1102-145 was prepared as a crude product (210 mg, 99 % yield) 20 from compound 1101-139 (215 mg, 0.5 mmol) using a procedure similar to that described for compound 1102-144 (Example 59): LCMS: 432 [M+1]+. Step 60b. 5-(4-(3-Chloro-4-fluorophenylamino)-7-methoxyquinazolin-6-yl)-N hydroxypentanamide (Compound 145) The title compound 145 was prepared as a white solid (90 mg, 43 %) from compound 25 1102-145 (210 mg, 0.5 mmol) and freshly prepared hydroxylamine in methanol (3.0 mL) using a procedure similar to that described for compound 144 (Example 59): 'H NMR (DMSO-d 6 ) 6 10.33 (s, 1H), 9.68 (s, 1H), 8.66 (s, 1H), 8.52 (s, 1H), 8.21 (s, 1H), 8.15-8.19 (m, 1H), 7.80-7.85 (m, 1H), 7.41 (t, J = 9.0Hz, 1H), 7.16 (s, 1H), 3.93 (s, 3H), 2.71 (t, J = 7.2 Hz, 2H), 1.95 (t, J = 7.2 Hz, 2H), 1.50-1.67 (m, 4H), 1.26-1.36 (m, 2H). LC-MS: 433 30 (M+1). EXAMPLE 61: Preparation of 4-(4-(3-chloro-4-fluorophenylamino)-7 methoxyquinazolin-6- ylthio)-N-hydroxybutanamide (Compound 149) 131 WO 20081033749 PCT/US2007/077977 Step 61a. S-4-(3-Chloro-4-fluorophenylamino)-7-methoxyquinazolin-6-yl benzothioate (Compound 1201) A mixture of compound 1001 (4.8 g, 1 1.4 mmol), thiobenzoic acid (7.8 g, 56.9 mol), 1,10-phenathroline (0.45 g, 2.3 mmol ), copper iodide (0.22 g, 1.1 mmol) and DIPEA (2.94 5 g, 22.8 mmol) in toluene (20 mL) was stirred at 1 10 C for 24 h under nitrogen atmosphere. After completion, the solvent was removed with reduced pressure and the residue was purified by column chromatography to get the crude target compound as a brown solid (1.0 g, 20%). LCMS: 440 [M+1]'-. Siep 61b. Ethyl 2

-(

4 -(3-chloro-4-fluorophenylamino)-7-methoxyquinazolin 10 6-ylthio)acetate (Compound 1202-149) A mixture of compd. 1201 ( 0.3 g, 0.68 mmol) and K 2 C0 3 ( 0.14 g, 1.0 mmol) in DMF was stirred at 50'C for 6 h under nitrogen. Ethyl 4-bromobutanoate (0.14 g, 0.71 mmol) was then added with a syringe and stirred for another 3 h. After the completion of the reaction, the solvent was removed with reduced pressure and the residue was purified by column 15 chromatography to give the target compound 1202-149 as a pale yellow solid (50 mg, 16%). LCMS: 450 [M+1] . Step 61c. 4

-(

4 -(3-Chloro-4-fluorophenylamino)-7-methoxyquinazolin-6 ylthio)-N-hydroxybutanamide (Compound 149) A mixture of compound 1202-149 (48 mg, 0.11 mmol) and freshly prepared NH 2 OH 20 methanol solution (1.77 M, 3.5 mL) was stirred for 30 min at room temperature. The mixture was adjusted to pH=7.0 with AcOH and the solvent was removed. The solid was collected and purified by column chromatography to give the target compound 149 as a pale yellow powder (14 mg, 30%). LCMS: 437.7 [M+1] ; 1 H NMR (DMSO-d) 5 10.72 (s, I H), 9.82 (s, 1H), 8.94 (s, 1H), 8.55 (s, 1H), 8.38 (m, 1H), 8.19 (s, 1H), 8.06 (m, 1H), 7.39 (m, 25 1H), 7.20 (s, 1H), 3.97 (s, 3H), 3.03 (m, 2H), 2.22 (in, 2H), 1.91 (brs, 2H). EXAMPLE 62: Preparation of 5-(4-(3-chloro-4-fluorophenylamino)-7 methoxyquinazolin-6-ylthio)-N-hydroxypentanamide (Compound 151) Step 62a. Ethyl 5-(4-(3-chloro-4-fluorophenylamino)-7-methoxyquinazolin-6 30 ylthio)pentanoate (Compound 1202-151) The title compound 1202-151 was prepared as a pale yellow solid (90 mg, 28 % yield) from compound 1201 (300 mg, 0.68 mmol) using a procedure similar to that described for compound 1202-149 (Example 61): LCMS: 464 [M+ 1]. 132 WO 2008/033749 PCT/US2007/077977 Step 62b. 5-(4-(3-Chloro-4-fluorophenylamino)-7-methoxyquinazolin-6-ylthio) N-hydroxypentanamide (Compound 151) The title compound 151 was prepared as a pale yellow powder (25 mg, 29 %) from compound 1202-151 (87 mg, 0.19 mmol) and freshly prepared hydroxylamine in methanol 5 (1.77M, 4.0 mL) using a procedure similar to that described for compound 149 (Example 61): LCMS: 451.7 [M+1] +; 'H NMR (DMSO-d) 6 10.74 (brs, 1H), 10.40 (s, 1H), 8.75 (s, 1H), 8.21 (s, 1H), 7.99 (m, 1H), 7.67 (m, 1H), 7.52 (in, 1H), 7.20 (s, 1H), 4.01 (s, 3H), 3.12 (brs, 2H), 2.00 (brs, 2H), 1.67 (brs, 4H). 10 EXAMPLE 63: Preparation of 5-(4-(3-chloro-4-fluorophenylamino)-7 methoxyquinazolin-6-ylthio)-N-hydroxypentanamide (Compound 155) Step 63a. Ethyl 2

-(

4 -(3-chloro-4-fluorophenylamino)-7-methoxyquinazolin 6-ylthio)acetate (Compound 1202-155) The title compound 1202-155 was prepared as a pale yellow solid (87 mg, 26 % yield) 15 from compound 1201 (300 mg, 0.68 mmol) using a procedure similar to that described for compound 1202-149 (Example 61): LCMS: 492 [M+1]*. Step 63b. 7

-(

4

-(

3 -Chloro-4-fluorophenylamino)-7-methoxyquinazolin-6-ylthio) N-hydroxyheptanamide (Compound 155) The title compound 155 was prepared as a pale yellow powder (28 mg, 34 %) from 20 compound 1202-155 (85 mg, 0.19 mmol) and freshly prepared hydroxylamine in methanol (1.77M, 4.0 mL) using a procedure similar to that described for compound 149 (Example 61): LCMS: 479.7 [M+1] +; 1 H NMR (DMSO-d 6 ) 5 10.32 (brs, 1H), 9.76 (s, 1H), 8.65 (s, lH), 8.51 (s, 1H), 8.14 (s, 1H), 8.09 (m, 1H), 7.75 (in, 1H), 7.44 (in, 1H), 7.19 (s, 1H), 3.97 (s, 3H), 3.08 (in, 2H), 1.92 (brs, 2H), 1.64 (brs, 2H), 1.45 (n, 4H), 1.28 (in, 2H). 25 EXAMPLE 64: Preparation of 7-(4-(3-chloro-4-fluorophenylamino)-6 methoxyquinazolin-7-yloxy)-N-hydroxyheptanamide (Compound 161) Step 64a. Ethyl 4 -(7-ethoxy-7-oxoheptyloxy)-3-hydroxybenzoate (Compound 1301-161) 30 To a solution of ethyl 3, 4-dihydroxybenzoate 0401 (6.0 g, 33 mmol) in DMF (50 ml) was added potassium carbonate (4.6 g, 33 mmol). The mixture was stirred at room temperature for 15 min, and then a solution of ethyl 7-bromoheptanoate (7.821 g, 33 mmol) in DMF (10 mL) was added dropwise. The mixture was stirred for 12 hours at 20'C. After 133 WO 2008/033749 PCT/US2007/077977 reaction the mixture was filtered, and the filtrate was concentrated in vacuo. The resulting residue was dissolved in dichloromethane and washed with brine. The organic phase was collected and dried over sodium sulfate, filtered and concentrated to give crude product. The crude product was purified by column chromatography (ethyl acetate/petroleum ether = 5 1:10) to give the title product 1301-161 as a white solid (2.44 g, 22%): LCMS: 338 [M+1] , 'H NMR (DMSO-d): 6 1.177 (t, J = 7.2 Hz, 3H), 1.247-1.438 (m, 7H), 1.480-1.553 (in, 2H), 1.579-1.754(m, 2H), 2.245-2.294 (t, J= 7.2 Hz, 2H), 3.972-4.063 (in, 4H), 4.190 4.261 (q, J= 7.2, 14.1 Hz 2H), 6.958-6.986 (d,.1 = 8.4 Hz, 1H), 7.358-7.404 (in, 2H), 9.36 (s, 1H). 10 Step 64b. Ethyl 4-(7-ethoxy-7-oxoheptyloxy)-3-methoxybenzoate (Compound 1302-161) Compound 1301-161 (1.2 g, 3.55 mmol), iodomethane (0.504 g, 3.55 mmol) and potassium carbonate (1.47 g, 10.65 mmol) in DMF (15 mL) was stirred at 80'C for 3 hours. After reaction the mixture was filtrated. The filtrate was concentrated in vacuo, and the resulting residue was dissolved in dichloromethane and washed with brine twice. The 15 organic phase was collected and dried over sodium sulfate, filtered and concentrated to give the title product 1302-161 as a white solid (1.2 g, 97%): LCMS: 353 [M+1], 'H NMR (DMSO-d): M1.131-1.178 (t, J= 6.9 Hz, 3H), 1.267-1.395 (in, 7H), 1.478-1.574 (m, 2H), 1.665-1.755 (m, 2H), 2.242-2.291 (t, J= 7.2 Hz, 2H), 3.792 (s, 3H), 3.982-4.063 (m, 4H), 4.229-4.300 (q, J= 7.2 Hz, 2H), 7.025-7.052 (d, J= 8.1 Hz, I H), 7.418-7.424 (d, J= 1.8 20 Hz, 1H), 7.529-7.562 (dd, J= 8.4 Hz, 1.8 Hz, IH). Step 64c. Ethyl 4 -(7-ethoxy-7-oxoheptyloxy)-5-methoxy-2-nitrobenzoate (Compound 1303-161) To a stirred solution of compound 1302-161 (1.2 g, 3.47 mmol) in acetic acid (10 mL) at 20'C was added fuming nitric acid (2.18 g, 34.7 mmol) dropwise. The reaction mixture 25 was stirred at 20'C for 1 hour and was then poured into ice-water and extracted with dichloromethane twice. The combined organic phase was washed with brine, aqueous NaHCO 3 solution and brine, and dried over sodium sulfate, filtered and concentrated to give the title product 1303-161 as a yellow oil (1.375 g, 98%): LCMS: 398 [M+1]. Step 64d. Ethyl 2 -amino-4-(7-ethoxy-7-oxoheptyloxy)-5-methoxybenzoate 30 (Compound 1304-161) A mixture of 1303-161 (1.375 g, 3.46 mmol), ethanol (30 mL), water (10 mL) and hydrogen chloride (1 mL) was stirred to form a clear solution. To the above solution was added powder iron (2.0 g, 34.6 mmol) potionwise. The mixture was stirred at reflux for 30 134 WO 2008/033749 PCT/US2007/077977 min, and was then cooled to room temperature. The pH of the reaction mixture was adjusted to 8 with the addition of 10% sodium hydroxide solution and filtered. The filtrate was concentrated to remove ethanol and then extracted with dichloromethane twice. The combined organic phase was washed with brine and dried over sodium sulfate, filtered and 5 concentrated to give the title product 1304-161 as a yellow solid (1.07 g, 84%): LCMS: 368 [M+1]+. Step 64e. Ethyl 7-(6-methoxy-4-oxo-3, 4-dihydroquinazolin-7-yloxy)heptanoate (Compound 1305-161) A mixture of compound 1304-161 (1.07 g, 2.92 mmol), ammonium fornate (0.184 g, 3 10 mmol) and formamide (10 mL) was stirred at 180 C for 3 hours. After reaction the mixture was cooled to room temperature. The formamide was removed under reduce pressure, and the residue was dissolved in dichloromethane and washed with brine. The organic phase was dried over sodium sulfate, filtered and concentrated to give the title product 1305-161 as a brown solid (0.684 g, 67%): LCMS: 349 [M+1]+. 15 Step 64f. Ethyl 7

-(

4 -chloro-6-methoxyquinazolin-7-yloxy)heptanoate (Compound 1306 161) A mixture of product 1305-161 (0.684 g, 1.97 mmol) and phosphoryl trichloride (20 mL) was stirred at reflux for 4 hours. After reaction the excessive phosphoryl trichloride was removed under reduced pressure and the residue was dissolved in dichloromethane and 20 washed with water, aqueous NaHCO 3 solution and brine. The organic phase was dried over sodium sulfate, filtered and concentrated to give the title product 1306-161 as a yellow solid (0.59 g, 82%): LCMS: 367 [M+1]-. Step 64g. Ethyl 7-(4-(3-chloro-4-fluorophenylamino)-6-methoxyquinazolin 7-yloxy)heptanoate (Compound 1307-161) 25 A mixture of 1306-161 (336 mg, 0.92 mmol) and 3-chloro-4- fluorobenzenamine (140 mg, 0.92 mmol) in isopropanol (10 mL) was stirred at reflux for 4 hours. After reaction the mixture was cooled to room temperature and resulting precipitate was isolated, washed with isopropanol and ether, and dried to give the title compound 1307-161 as a yellow solid (389 mg, 89%): LCMS: 476 [M+1]. 30 Step 64h. 7-(4-(3-chloro- 4 -fluorophenylamino)-6-methoxyquinazolin-7 yloxy)-N-hydroxyheptanamide (Compound 161) To a freshly prepared hydroxylamine solution (2.5 mL, 3.75 mmol) was added compound 1307-161 (359 mg, 0.75 mmol). The resulting reaction mixture was stirred at 135 WO 20081033749 PCT/US2007/077977 25'C for 24 hours. After reaction the mixture was neutralized with acetic acid, and resulting precipitate was isolated, washed with water, and dried to give the title compound 161 as a white solid (60 mg, 17%): mp 238.5~253.4 0 C, LCMS: 463 [M+1]', ' H NMR (DMSO-d): 31.23-1.55 (in, 6H), 1.76-1.8 (in, 2H), 1.96 (t, J= 7.2 Hz, 2H), 3.96 (s, 3H), 4.13 (t, J= 6.3 5 Hz, 2H), 7.19 (s, 1H), 7.20 (in, 2H), 7.46 (t, J= 9 Hz, 1H), 7.78 (d, J=7.5 Hz, 1H), 8.12 8.15 (dd, J= 2.4, 6.9 Hz, 1H), 8.50 (s, 1H), 8.67 (s, 1H), 9.57 (s, 1H), 10.35 (s, 1H). EXAMPLE 65: Preparation of 7-(4-(3-ethynylphenylamino)-6 methoxyquinazolin-7-yloxy)-N-hydroxyheptanamide (Compound 162) 10 Step 65a. Ethyl 7-(4-(3 -ethynylphenylamino)-6-methoxyquinazolin-7-yloxy) heptanoate (Compound 1307-162) The title compound 1307-162 was prepared as a yellow solid (253 mg, 46 % yield) from compound 1306-162 (446 mg, 1.22 mmol), 3-ethynylbenzenamine (142 mg, 1.22 mmol) and i-propanol (10 mL) using a procedure similar to that described for compound 15 1307-161 (Example 64): LCMS: 448 [M+1]*. Step 65b.7-(4-(3-ethynylphenylamino)-6-methoxyquinazolin-7 yloxy)-N-hydroxyheptanamide (Compound 162) The title compound 162 was prepared as a yellow powder (20 mg, 8 %) from compound 1307-161 (246 mg, 0.0.55 mmol) and freshly prepared hydroxylamine in 20 methanol (2.0 mg, 2.75 mmol) using a procedure similar to that described for compound 161 (Example 64): LCMS: 435 [M+1]+, 'H NMR (DMSO-d 6 ): 31.301-1.541 (m, 6H), 1.740-1.792 (m, 2H), 1.929-1.977 (m, 2H), 3.959 (s, 3H), 4.123 (t, J= 6.6 Hz, 2H), 4.192 (s, 1H), 7.176-7.221 (m, 2H), 7.360-7.427 (m, 1H), 7.831-7.890 (in, 2H), 7.966 (m, 1H), 8.504 (s, IH), 8.642 (s, 1 H), 9.547 (s, 1 H), 10.321 (s, 1 H). 25 EXAMPLE 66: Preparation of 7-(4-(3-chloro-4-fluorophenylamino)-6-(2 methoxyethoxy)quinazolin-7-yloxy)-N-hydroxyheptanamide (Compound 167) Step 66a. Ethyl 4 -(7-ethoxy-7-oxoheptyloxy)-3-(2-methoxyethoxy) benzoate (Compound 1302-167) 30 The title compound 1302-167 was prepared as a yellow solid (1400 mg, 97 % yield) from compound 1301 (1223 mg, 3.62 mmol), 2-methoxyethyl 4-methylbenzenesulfonate (0.834, 3.62 mmol), DMF (15 mL) and potassium carbonate (1.50 g, 10.86 mmol) using a procedure similar to that described for compound 1302-161 (Example 64): LCMS: 397 136 WO 20081033749 PCT/US20071077977 [M+1],IH NMR (DMSO-d): 31.152 (t, J= 7.2 Hz, 3H), 1.264-1.405 (in, 7H), 1.478-1.572 (in, 2H), 1.663-1.730 (in, 2H), 2.267 (t, J= 7.2 Hz, 2H), 3.315 (s, 3H), 3.650 (t, J= 5.4 Hz, 21H), 3.990-4.062 (m, 4H), 4.089-4.119 (m, 3H), 4.222-4.293 (q, J= 7.2 Hz, 21-), 7.053 (d,,J = 8.1 Hz, 1H), 7.447-7.486 (in, 1H), 7.539-7.567 (dd, J= 8.4 Hz, 1.8 Hz, 1H). 5 Step 66b. Ethyl 4-(7-ethoxy-7-oxoheptyloxy)-5-(2-methoxyethoxy)-2-nitrobenzoate (Compound 1303-167) The title compound 1303-167 was prepared as a yellow oil (1510 mg, 97 % yield) from compound 1302-167 (1400 mg, 3.5 mmol), acetic acid (10 mL) and fuming nitric acid using a procedure similar to that described for compound 1303-161 (Example 64): LCMS: 10 442 [M+1]. Step 66c. Ethyl 2-amino-4-(7-ethoxy-7-oxoheptyloxy)-5-(2-methoxyethoxy) benzoate (Compound 1304-167) The title compound 1304-167 was prepared as a yellow oil (1210 mg, 97 % yield) from compound 1303-167 (1500 mg, 3.4 mmol), powder iron (1.9 g, 34 mmol), ethanol (30 mL), 15 water (10 mL) and hydrogen chloride (1 mL) using a procedure similar to that described for compound 1304-161 (Example 64): LCMS: 412 [M+1]. Step 66d. Ethyl 7-(6-(2-methoxyethoxy)-4-oxo-3, 4-dihydroquinazolin-7-yloxy) heptanoate (Compound 1305-167) The title compound 1305-167 was prepared as a yellow solid (859 mg, 85 % yield) 20 from compound 1304-167 (1210 mg, 2.9 mmol), ammonium formate (0.184 g, 3 mmol) and formamide (10 mL) using a procedure similar to that described for compound 1305-161 (Example 64): LCMS: 393 [M+I]-. Step 66e. Ethyl 7-( 4 -chloro-6-(2-methoxyethoxy)quinazolin-7-yloxy)heptanoate (Compound 1306-167) 25 The title compound 1306-167 was prepared as a yellow solid (572 mg, 63 % yield) from compound 1305-167 (859 mg, 2.2 mmol) and phosphoryl trichloride (20 mL) using a procedure similar to that described for compound 1306-161 (Example 64): LCMS: 411 [M+1]. Step 66f. Ethyl 7-(4-(3-chloro-4-fluorophenylamino)-6-(2- methoxyethoxy) 30 quinazolin-7-yloxy) heptanoate (Compound 1307-167) The title compound 1307-167 was prepared as a yellow solid (238 mg, 76 % yield) from compound 1306-167 (251 mg, 0.6 mmol), 3-chloro-4-fluorobenzenamine (90 mg, 0.6 137 WO 2008/033749 PCT/US2007/077977 mmol) and i-propanol (5 mL) using a procedure similar to that described for compound 1307-161 (Example 64): LCMS: 520 [M+1]*. Step 66g. 7-(4-(3-chloro-4-fluorophenylamino)-6-(2-methoxyethoxy)quinazolin 7-yloxy)-N-hydroxyheptanamide (Compound 167) 5 The title compound 167 was prepared as a yellow solid (20 mg, 9 % yield) from compound 1307-167 (232 mg, 0.45 mmol) and) and freshly prepared hydroxylamine solution (2 mL, 2.1 mmol) using a procedure similar to that described for compound 161 (Example 64): LCMS: 507 [M+-1], 1 H NMR (DMSO-d 6 ): 1.314-1.539 (m, 6H), 1.754 1.801 (in, 2H), 1.926-1.975 (in, 2H), 3.368 (s, 3H), 3.770 (t, J= 4.8 Hz, 2H), 4.135 (t, J= 10 6.3 Hz, 2H), 4.267 (t, J= 4.8 Hz, 2H), 7.19 (s, 1H), 7.440 (t, J= 8.4 Hz, IH), 7.764-7.833 (in, 2H), 8.095-8.126 (dd, J= 2.7, 6.9 Hz, 1H), 8.499 (s, IH), 8.612 (s, 1H), 8.635 (s, 1H), 9.555 (s, 1H), 10.314 (s, 1H). EXAMPLE 67: Preparation of 7-(4-(3-ethynylphenylamino)-6-(2-methoxyethoxy) 15 quinazolin-7-yloxy)-N-hydroxyheptanamide (Compound 168) Step 67a. Ethyl 7-(4-(3-ethynylphenylamino)-6-(2-methoxyethoxy) quinazolin 7-yloxy)Heptanoate (Compound 1307-168) The title compound 1307-168 was prepared as a yellow solid (214 mg, 56 % yield) from compound 1307-167 (320 mg, 0.78 mmol), 3-ethynylbenzenamine (92 mg, 0.78 20 mmol), i-propanol (5 mL):using a procedure similar to that described for compound 1307 161 (Example 64): LCMS: 520 [M+1]'-. Step 67b. 7-(4-(3-ethynylphenylamino)-6-(2-methoxyethoxy)quinazolin 7-yloxy)-N-hydroxyheptanamide (Compound 168) The title compound 168 was prepared as a yellow solid (30 mg, 15 % yield) from compound 25 1307-178 (204 mg, 0.42 mmol) and) and freshly prepared hydroxylamine solution (2 mL, 2.1 mmol) using a procedure similar to that described for compound 161 (Example 64): LCMS: 479 [M+1]f, 1 H NMR (DMSO-d): 61.314-1.539 (m, 6H), 1.754-1.800 (m, 2H), 1.925-1.975 (in, 2H), 3.370 (s, 3H), 3.771 (t, J= 4.8 Hz, 2H), 4.131 (t, J= 6.3 Hz, 2H), 4.186 (s, 1H), 4.275 (t,.1 = 4.8 Hz, 2H), 7.19 (d, J= 7.5 Hz, 2H), 7.390 (t, .= 7.8 Hz, 1H), 30 7.847-7.900 (in, 2H), 7.975 (s, 1H), 8.487 (s, 1H), 8.636 (s, 1H), 9.455 (s, 1H), 10.316 (s, 111). 138 WO 2008/033749 PCT/US2007/077977 Example 68 :Preparation of 3-((5-(4-(3-chloro-4-(3-fluorobenzyloxy) phenylamino)quinazolin-6-yl)furan-2-yl)methylamino)-N hydroxypropanamide (Compound 174) Step 68a. Methyl 2-amino-5-iodobenzoate (Compound 1402-174) 5 Methyl 2-aminobenzoate (23g, 15.2 mmol) was dissolved in 200 mL of water and 32 mL of concentrated hydrochloric acid; the solution was cooled to 20'C. A solution of iodine monochloride in hydrochloric acid is prepared by diluting 28 mL of concentrated hydrochloric acid with 100 niL of cold water, adding just sufficient crushed ice to bring the temperature to 5 "C, and, during about two minutes, stirring in monochloride ( 2 5g, 15.5 10 mmol). The iodine monochloride solution is stirred rapidly into the methyl 2-aminobenzoate solution. Methyl 2-amino-5-iodobenzoate separates almost immediately as a granular, tan to violet precipitate. The mixture is stirred for an hour, then filtered, washed with cold water, and then dried in vacuum to yield the 1402-174 as a solid (17.8 g, 42 %): LC-MS: 278 [M+1]*, 1 H NMR (DMSO-d 6 ): d 3.70 (s, 3 H), 6.64 (d, J= 9.0 Hz, 1 H)O 6.78 (b, 2H), 7.47 15 (dd, Ji = 9.0 Hz, 12 = 1.8 Hz, I H), 7.90 (d, J= 1.8 Hz, 1 H). Step 68b. 6-Iodoquinazolin-4(3H)-one (Compound 1403-174) Methyl 2-amino-5-iodobenzoate (17.8 g, 64 mmmol) was heated in 300 mL of formamide at 190'C for 2 hours. The mixture was cooled to room temperature and the solid product was filtrated and dried in vacuum. The formed product 1403-174 was used without 20 further purification.(10 g, 56.1%): LC-MS: 273 [M+I]+, 'H NMR (DMSO- 6 1): 3 7.46 (d, J = 9.0 Hz, 1 H), 8.10 (in, 2H), 8.36 (d, J= 2.1 Hz, 1 H), 12.40 (s, 1 H). Step 68c. 4-Chloro-6-iodoquinazoline (Compound 1404-174) 6-Iodoquinazolin-4(3H)-one (lOg, 37 mmol) was refluxed in POCl 3 (100 mL) overnight. Then POC 3 was removed in vacuo. The residue was dissolved in CH 2 Cl 2 (500 25 mL). The organic phase was washed with water (100 mL) and dried (MgSO 4 ). Then CH 2 Cl 2 was removed in vacuo and 1404-174 was obtained (5.7 g, 53%): LC-MS: 291 [M+1]', 'H NMR (CDCl 3 ): 3 7.81 (d, J = 9.0 Hz, 1 H), 8.21 (dd, Ji = 9.0 Hz, J 2 = 1.8 Hz, 1 H), 8.65 (d, J= 1.8 Hz, 1 H), 9.06 (s, 1 H). Step 68d. Synthesis of N-(3-chloro-4-(3-fluorobenzyloxy) phenyl) 30 6-iodoquinazolin-4-amine (Compound 1405-174) 4-Chloro-6-iodoquinazoline (5.7g, 19.7 mmol) and 3-chloro-4-(3 fluorobenzyloxy)aniline (4.9g, 19.7 mmol) was refluxed in isopropanol (1 50mL) overnight. The mixture was cooled to room temperature. The solid product was precipitated, filtrated 139 WO 2008/033749 PCT/US2007/077977 and dried in vacuum. The product 1405-174 was pure enough and used without further purification.(7.4 g, 74.2%): LC-MS: 506 [M+1]+,'H NMR (DMSO-d 6 ): o 5.29 (s, 2 H), 7.18 (m, 111), 7.33 (in, 3H), 7.48 (m, 1 I), 7.66 (in, I H), 7.74 (d, J= 9.0 11z, 1 H), 7.90 (d, J = 2.2 Hz, 1 H), 8.37 (d, J= 9.0 Hz, 1 H), 8.94 (s, I H), 9.29 (s, 1 H). 5 Step 68e. 5-(4-(3-Chloro-4-(3-fluorobenzyloxy)phenylamino) quinazolin-6-yl)furan-2-carbaldehyde (Compound 1406-174) N-(3-Chloro-4-(3-fluorobenzyloxy)phenyl)-6-iodoquinazolin-4-amine (387 mg, 0.77 mmol) and 5-formylfuran-2-ylboronic acid (129 mg, 0.92 mmol) were added into the mixture of THF (10 mL) , ethanol (5 mL) and Et 3 N (0.3 mL) under N 2 atmosphere. Then 10 PdCl 2 (dppf) (26 mg, 0.03 mmol) was added into the mixture. The mixture was refluxed overnight. Then the solvent was removed in vacuo, the residue was chromatographed on silica gel with ethyl acetate to give product 1406-174 (240 mg, 66.2%): LC-MS: 474 [M+1]+, 'H NMR (DMSO-d): 0 5.20 (s, 2 H), 7.17 (m, 1H), 7.29 (in, 3H), 7.41 (in, 2H), 7.74 (in, 2H), 7.86 (d, J= 9.0 Hz, 1 H), 7.97 (s,1 H), 8.31 (d, J= 9.0 Hz, 1 H), 8.56 (s, I H), 15 8.96 (s, 1 H), 9.66 (s, 1 H), 10.11 (s, 1 H). Step 68f. Ethyl 3 -((5-(4-(3-chloro-4-(3-fluorobenzyloxy)phenylamino) quinazolin-6-yl)furan-2-yl)methylamino)propanoate (Compound 1407-174) Compound 1406-174 (240 mg, 0.5 mmol) and ethyl 3-aminopropanoate hydrochloride (77 mg, 0.5 mmol) were dissolved in 10 mL of THF, then Et 3 N (0.1 mL)was added. The 20 mixture was stirred for 10 min. and then NaBH(AcO) 3 (148 mg, 0.7 mmol) was added into the mixture. The mixture was stirred for another 1 hour. The solvent was removed, and the residue was purified by chromatography on silica gel with CH 2 Cl 2 / MeOH (100:5) to give product 1407-174 (140 mg, 47.9%): LC-MS: 575 [M+1]', 1H NMR (DMSO-d): 6 1.13 (t, J = 6.9 Hz, 3 H), 2.43 (in, 2 H), 2.80 (t, J= 6.9 Hz, 3 H), 3.76 (s, 2 H), 4.01 (q, J= 6.9 Hz, 2 25 H), 5.21 (s, 2 H), 6.46 (s, 1 H), 7.03 (in, 1 H), 7.16 (in, 1 H), 7.30 (in, 3 H), 7.46 (m, 1 H), 7.82 (in, 2 H), 8.03 (in, 1 H), 8.14 (in, 1 H), 8.52 (s, 1 H), 8.71 (s, 1 H), 9.90 (s, 1 H). Step 68g. 3-((5-(4-(3-Chloro-4-(3- fluorobenzyloxy)phenylamino)quinazolin-6-yl) furan-2-yl)methylamino)-N-hydroxypropanamide (Compound 174) Compound 1407-174 (110 ing, 0.19mmol) was dissolved in freshly made NH 2 OH 30 methanol solution (1mL, 1.76mol/L). The mixture was stirred for 30 min. and the reaction was monitored by TLC. HOAc was added to adjust the pH of the reaction mixture to 7. The solvent was removed in vacuo and the residue was washed with water (10mL). The product was purified by preparative liquid chromatography to yield compound 174 as a yellow solid 140 WO 2008/033749 PCT/US2007/077977 (41 mg, 37.2%): Mp.170'C. LC-MS: 562 [M+1] t , 'H NMR (DMSO-d 6 ): 6 2.14 (t, J= 6.9 Hz, 2 H), 2.77 (t, J= 6.9 Hz, 2 H), 3.79 (s, 2 H), 5.25 (s, 2 H), 6.45 (d, J= 3.0 Hz, I H), 7.03 (d,,J= 3.0 Hz, 1 1-1), 7.18 (in, 1 H), 7.31 (in, 3 H), 7.45 (in, 1 ), 7.72 (in, 2 H), 8.00 (in, 1 H), 8.15 (d, J= 7.5 Hz, 1 H), 8.53 (s, 1 H), 8.71 (s, 2 H), 9.92 (s, I H). 5 EXAMPLE 69: Preparation of 6-((5-(4-(3-chloro-4-(3-fluorobenzyloxy) phenylamino)quinazolin-6-yl)furan-2-yl)methylamino)-N-hdroxyhexanamide (Compound 177) Step 69a. Methyl 6 -((5-( 4 -(3-chloro-4-(3-fluorobenzyloxy)phenylamino) 10 quinazolin-6-yl)furan-2-yl)methylamino)hexanoate (Compound 1407-177) The title compound 1407-177 was prepared (260 mg, 21.6 % yield) from compound 1406-174 (960 mg, 2.0 mmol) and methyl 6-aminohexanoate hydrochloride (362 mg, 2 mmol) using a procedure similar to that described for compound 1407-174 (Example 68): LCMS: 603 [M+1]. 15 Step 69b. 6-((5-(4-(3-Chloro-4-(3-fluorobenzyloxy)phenylamino)quinazolin 6 -yl)furan-2-yl)methylamino)-N-hydroxyhexanamide (Compound 177) The title compound 177 was prepared as a white solid (22 mg, 22 % yield) from compound 1407-177 (100 mg, 0.17 mmol) and freshly prepared hydroxylamine solution (1 mL, 1.76mol/L) using a procedure similar to that described for compound 174 (Example 20 68): Mp. 121'C. LC-MS: 604 [M+1]+, 'H NMR (DMSO-d 6 ): 6 1.03 (t, J= 6.0 Hz, 2 H), 1.18 (in, 2 H), 1.47 (in, 4 H), 1.92 (t, J= 6.0 Hz, 2 H), 2.54 (in, 2 H), 3.41 (s, 1 H), 3.78 (s, 2 H), 5.26 (s, 2 H), 6.40 (s, 1 H), 7.02 (s, 1 H), 7.17 (m, I H), 7.29 (m, 3 H), 7.46 (in, 1 H), 7.76 (n, 2 H), 7.99 (s, 1 H), 8.16 (d, J= 8.1 Hz, 1 H), 8.53 (s, 1 H), 8.70 (in, 2 H), 9.90 (s, 1 H), 10.33 (s, 1 H). 25 EXAMPLE 70: Preparation of 7-((5-(4-(3-chloro-4-(3-fluorobenzyloxy) phenylamino)quinazolin-6-yl)furan-2-yl)methylamino)-N-hydroxyheptanamide (compound 178) Step 70a. Ethyl 7 -((5-( 4

-(

3 -chtoro-4-(3-fluorobenzyloxy)phenylamino) 30 quinazolin- 6 -yl)furan-2-yl)methylamino)heptanoate (Compound 1407-178) The title compound 1407-178 was prepared (270 mg, 21.4 % yield) from compound 1406-174 (960 mg, 2.0 mmol) and methyl ethyl 7-aminoheptanoate hydrochloride 141 WO 2008/033749 PCT/US2007/077977 hydrochloride (418 mg, 2 mmol) using a procedure similar to that described for compound 1407-174 (Example 68): LCMS: 631 [M+1]*. Step 70b. 7-((5-( 4 -(3-Chloro-4-(3-fluorobenzyloxy)phenylamino)quinazolin 6-yl)furan-2-yl)methylamino)-N-hydroxyheptanamide (Compound 178) 5 The title compound 178 was prepared as a white solid (25 mg, 25 % yield) from compound 1407-178 (110 mg, 0.17 mmol) and freshly prepared hydroxylamine solution (1 mL, 1.76mo1/L) using a procedure similar to that described for compound 174 (Example 68): Mp. 120 0 C. LC-MS: 618 [M+1]+, 1 H NMR (DMSO-d): 6 1.22 (in, 4H), 1.42 (m, 4H), 1.90 (t, J= 7.5 Hz, 2 H), 2.54 (in, 2H), 3.76 (s, 2 H), 5.24 (s, 2 H), 6.42 (d, J= 3.0 Hz, 1 H), 10 7.01 (d, J= 3.0 Hz, I H),, 7.19 (in, 1 H), 7.31 (in, 3 H), 7.44 (in, 1 H), 7.70 (in, 2 H), 7.99 (s, 1 H), 8.14 (in, 1 H), 8.52 (s, 1 H), 8.69 (in, 2 H), 9.89 (s, 1 H), 10.30 (s, I H). EXAMPLE 71. Preparation of 7-(4-(3-chloro-4-(3- fluorobenzyloxy) phenylamino)quinazolin-6-yloxy)-N-hydroxyheptanamide (Compound 198) 15 Step 71a. 2-Chloro-1-(3-fluorobenzyloxy)-4-nitrobenzene (Compound 1502) A mixture of 2-chloro-4-nitrophenol (35 g, 0.2 mol), n-furobenzylbromide (45.4 g, 0.24 mol), K 2

CO

3 (55.2 g, 0.4 mol) and acetone (800 mL) was stirred at 30 *C for 16h. The resulting mixture was filtered and washed with acetone. The filtrate was concentrated to give the crude product which was washed with petroleum ether and dried to give the 20 product 1502 as a yellow solid (55.0 g, 99% yield). 1 H NMR (DMSO-d 6 ): 6 8.33 (d, J = 3.3 Hz, 1H), 8.21-8.26 (in, 1H), 7.42-7.50 (in, 2H), 7.29-7.33 (in, 2H), 7.16-7.22 (in, 1H), 5.39 (s, 2H). LC-MS: 282 (M+1). Step 71b. 3 -Chloro-4-(3-fluorobenzyloxy)benzenamine (Compound 1503) A mixture of 1502 (15 g, 53.4 mmol), iron powder (30 g, 0.534 mol), concentrated 25 hydrochloric acid (5.4 mL), ethanol (360 mL) and water (120 mL) was refluxed for 2 h. The hot solution was then filtered and the filtrate was concentrated to give the product 1503 as a solid (11.0 g, 82% yield). 1 H NMR (DMSO-d): 6 7.37-7.45 (in, 1 H), 7.21-7.26 (m, 2H), 7.09-7.16 (in, 1H), 6.90 (d, J = 8.7 Hz, 1H), 6.63-6.34 (in, 1H), 6.44 (dd, J 1 , J 2 8.7 Hz, 1.8 Hz, 1H), 5.01 (s, 2H), 4.94 (s, 2H). LC-MS: 252 (M+1). 30 Step 71c. Acetic acid 4 -[3-chloro-4-(3-fluoro-benzyloxy)-phenylamino] quinazolin-6-yl ester (Compound 1504-198) A mixture of compound 0204 (Scheme 2) (0.85 g, 3.8 mmol) and 3-chloro-4- 3 fluorobenzyloxy)benzenamine (1503) (1.26 g, 5.0 mmol) in isopropanol (20 mL) was 142 WO 2008/033749 PCT/US2007/077977 stirred and heated at 90"C for 20 minutes. The reaction was cooled to room temperature and the precipitate was isolated. The solid was washed with isopropanol and methanol, dried to provide the title compound 1504-198 as a dark yellow solid (1.5 g, 90%). LC-MS: 438[M+1]+. 5 Step 71d. 4-[3-Chloro-4-(3-fluoro-benzyloxy)-phenylamino]-quinazolin-6-ol (Compound 1505-198) A mixture of compound 1504-198 (1.5 g, 3.4 mmol) and lithium hydroxide onohydrate (0.29 g, 6.9 mmol) in methanol (40 mL) / water (40 mL) was stirred at room temperature for 4 hours. The pH was adjusted to 4 with acetic acid and filtered. The collected yellow solid 10 was washed by water and dried to obtained title compound 1505-198 as a yellow solid (1.2 g, 89%). LC-MS: 395[M+1]+. Step 71e. 7-{ 4

-[

3 -Chloro-4-(3-fluoro-benzyloxy)-phenylamino]-quinazolin 6-yloxy}-heptanoic acid ethyl ester (Compound 1506-198) A mixture of compound 1505-198 (0.12 g, 0.30 mmol), ethyl 3-bromopropanoate 15 (72 mg, 0.30 mmol) and K 2 C0 3 (165 mg, 1.2 mmol) in DMF (5mL) was stirred and heated to 60 'C overnight. The reaction was filtered and the filtrate was evaporated. The resulting solid was washed with ether and purified by TLC to obtain the title compound 1506-198 as a yellow solid (80 mg, 48%). LC-MS: 551 [M+1]: 1 H NMR (DMSO-d): c 1.15 (t, J= 7.5 Hz, 3H), 1.46 (m, 8H), 1.79 (in, 2H), 2.29(t, J= 7.2 Hz, 2H), 3.24(s, 1 H), 4.02(d, 11= 6.6 20 Hz, J2 = 14.4 Hz, 2H), 4.12 (t, J= 6.3 Hz, 2H), 5.24(s, 2H), 7.15 (m, 1H), 7.45 (in, 3H), 7.48 (in, 2H), 7.85 (d, J= 2.7 Hz, 1H), 7.98 (d, J= 2.7 Hz, 1H), 8.47 (s, 1H), 9.57 (s, 1H). Step 71f. 7-{4-[3-Chloro-4-(3-fluoro-benzyloxy)-phenylamino]-quinazolin-6-yloxy} heptanoic acid hydroxyamide (Compound 198) To compound 1506-198 (70 mg, 0.13 mmol) was added the freshly prepared 25 hydroxylamine methanol solution (0.5 mL, 0.89 mmol). The reaction process was monitored by TLC. After completion of the reaction, the mixture was neutralized with acetic acid and concentrated under reduce pressure to a residue which was washed by water to give the title compound 198 as a yellow solid (35 mg, 46%): LC-MS: 539 [M+I]; 1 H NMR (DMSO-d 6 ): 1.50 (in, 8H), 1.79(t,.J= 6.6 Hz, 2H), 3.24(s, 1H), 1.95(m, 2H), 4.12 30 (t, J= 5.1 Hz, 2H), 5.24(s, 2H), 7.15 (i, 1H), 7.45 (m, 3H), 7.48 (in, 2H), 7.70 (d, J= 2.7 Hz, 1H), 7.87 (s, 1H), 7.97 (s, 1H), 8.50 (s, 1H), 8.67 (s, 1H), 9.70(s, 1H), 10.35 (s, 1H). 143 WO 2008/033749 PCT/US2007/077977 EXAMPLE 720Preparation of 7

-(

4

-(

3 -chloro-4-(3-fluorobenzyloxv) phenylamino)-7-methoxyquinazolin-6-yloxy) -N-hydroxyheptanamide (Compound 199) Step 72a. 4

-(

3 -Chloro-4-(3-fluorobenzyloxy)phenylaino)-7-methoxyquinazolin-6-yl 5 acetate (Compound 1504-199) A mixture of 0105 (Scheme 1) (253 mg, 1.0 mmol) and 1503 (252 mg, 1.0 mmol) in isopropanol (10 mL) was stirred and heated to reflux for I hours. The mixture was cooled to room temperature and resulting precipitate was isolated. The solid was dried to give the title compound 1504-199 as a pale solid (420 mg, 90%): LCMS: 468 [M+1] . 10 Step 72b. 4-(3-Chloro-4-(3-fluorobenzyloxy)phenylamino)-7-methoxyquinazolin-6-ol (Compound 1505-199) A mixture of compound 1504-199 (418 mg, 0.89 mmol), LiOH-H 2 0 (126 mg, 3.0 mmol) in methanol (20 mL) and H 2 0 (10 mL) was stirred at room temperature for 10 mi. The mixture was neutralized by addition of dilution acetic acid. The precipitate was isolated 15 and dried to give the title compound 1505-199 as a pale white solid (376 mg, 99%): LCMS: 426 [M+1 ]; 'H NMR (DMSO-d 6 ): ( 3.97 (s, 3H), 5.24 (s, 2H), 7.19 (m, 3H), 7.32 (in, 2H), 7.48 (m, 1H), 7.74 (in, 2H), 8.04 (d, J= 2.4 Hz, 1H), 8.43 (s, 1H), 9.35 (s, 1H), 9.66 (s, 1H). Step 72c. Ethyl 7

-(

4

-(

3 -chloro-4-(3-fluorobenzyloxy)phenylamino)-7- methoxyquinazolin 6 -yloxy) heptanoate (Compound 1506-199) 20 A mixture of compound 1505-199 (170 mg, 0.4 mmol), ethyl 7-bromoheptanoate (95 mg, 0.4 mmol) and potassium carbonate (166 mg, 1.2 mmol) in N, N-dimethylformamide (10 mL) was stirred and heated to 70 0 C for 4 hours. The reaction mixture was filtrated. The filtrate was concentrated under reduce pressure. The residues was suspended in water, the precipitate was collected and dried to give the title compound 1506-199 as a yellow solid 25 (89 mg, 38%): LCMS: 582 [M+I]-. Step 72d. 7

-(

4

-(

3 -Chloro-4-(3-fluorobenzyloxy)phenylamino)-7- methoxyquinazolin-6 yloxy)-N-hydroxyheptanamide (Compound 199) A mixture of compound 1506-199 (88 mg, 0.15 mmol) and freshly prepared 1.77 mol/L

NH

2 OH/MeOH (3 mL, 5.3 mmol) was stirred at room temperature for 0.5 h. The reaction 30 mixture was neutralized with AcOH, the precipitate was isolated and dried to give the title compound 199 as a pale yellow solid (48 mg, 56%): LCMS: 569 [M+1] , 'H NMR (DMSO d 6 ): J 1.35 (m, 2H), 1.50 (m, 4H), 1.83 (m, 2H), 1.98 (m, 2H), 3.94 (s, 3H), 4.13 (m, 2H), 144 WO 20081033749 PCT/US2007/077977 5.26 (s, 2H), 7.19 (in, 2H), 7.36 (m, 3H), 7.48 (m, 1H), 7.69 (m, 1H), 7.80 (s, 1H), 7.95 (d, J = 2.7 Hz, 1H), 8.45 (s, 1H), 8.68 (s, 1H), 9.43 (s, IH), 10.36 (s, IH). Biological Assays: 5 As stated hereinbefore the derivatives defined in the present invention possess anti proliferation activity. These properties may be assessed, for example, using one or more of the procedures set out below: (a) An in vitro assay which determines the ability of a test compound to inhibit EGFR kinase. 10 The ability of compounds to inhibit receptor kinase (EGFR) activity was assayed using HTScanTM EGF Receptor Kinase Assay Kits (Cell Signaling Technologies, Danvers, MA). EGFR tyrosine kinase was obtained as GST-kinase fusion protein which was produced using a baculovirus expression system with a construct expressing human EGFR (His672-Alal210) (GenBank Accession No. NM_005228) with an amino-terminal GST tag. 15 The protein was purified by one-step affinity chromatography using glutathione-agarose. An anti-phosphotyrosine monoclonal antibody, P-Tyr-100, was used to detect phosphorylation of biotinylated substrate peptides (EGFR, Biotin-PTPlB (Tyr66). Enzymatic activity was tested in 60 mM HEPES, 5 mM MgCl 2 5 mM MnCl 2 200 gM ATP, 1.25 mM DTT, 3 pM Na 3

VO

4 , 1.5 mM peptide, and 50 ng EGF Recpetor Kinase. Bound 20 antibody was detected using the DELFIA system (PerkinElmer, Wellesley, MA) consisting of DELFIA@ Europium-labeled Anti-mouse IgG (PerkinElmer, #AD0124), DELFIA@ Enhancement Solution (PerkinElmer, #1244-105), and a DELFIA@ Streptavidin coated, 96 well Plate (PerkinElmer, AAAND-0005). Fluorescence was measured on a WALLAC Victor 2 plate reader and reported as relative fluorescence units (RFU). Data were plotted 25 using GraphPad Prism (v4.0a) and IC50's calculated using a sigmoidal dose response curve fitting algorithm. Test compounds were dissolved in dimethylsulphoxide (DMSO) to give a 20 mM working stock concentration. Each assay was setup as follows: Added 100 pl of 10 mM ATP to 1.25 ml 6 mM substrate peptide. Diluted the mixture with dH 2 0 to 2.5 ml to make 30 2X ATP/substrate cocktail ([ATP]=400 mM, [substrate] =3 mM). Immediately transfer enzyme from -80'C to ice. Allowed enzyme to thaw on ice. Microcentrifuged briefly at 4'C to bring liquid to the bottom of the vial. Returned immediately to ice. Added 10 pl of DTT (1.25 mM) to 2.5 ml of 4X HTScanTM Tyrosine Kinase Buffer (240 mM HEPES pH 7.5, 145 WO 2008/033749 PCT/US2007/077977 20 mM MgC1 2 , 20 mM MnCI, 12 mM NaVO 3 ) to make DTT/Kinase buffer. Transfer 1.25 ml of DTT/Kinase buffer to enzyme tube to make 4X reaction cocktail ([enzyme] = 4 ng/tIL in 4X reaction cocktail). Incubated 12.5 pl of the 4X reaction cocktail with 12.5 pl/well of prediluted compound of interest (usually around 10 pM) for 5 minutes at room temperature. 5 Added 25 pl of 2X ATP/substrate cocktail to 25 pl/well preincubated reaction cocktail/compound. Incubated reaction plate at room temperature for 30 minutes. Added 50 pl/well Stop Buffer (50 mM EDTA, pH 8) to stop the reaction. Transferred 25 l of each reaction and 75 lu dH 2 O/well to a 96-well streptavidin-coated plate and incubated at room temperature for 60 minutes. Washed three times with 200 pl/well PBS/T (PBS, 0.05% 10 Tween-20). Diluted primary antibody, Phospho-Tyrosine mAb (P-Tyr-100), 1:1000 in PBS/T with 1% bovine serum albumin (BSA). Added 100 pl/well primary antibody. Incubated at room temperature for 60 minutes. Washed three times with 200 pl/well PBS/T. Diluted Europium labeled anti-mouse IgG 1:500 in PBS/T with 1% BSA. Added 100 pl/well diluted antibody. Incubated at room temperature for 30 minutes. Washed five times 15 with 200 tl/well PBS/T. Added 100 pl/well DELFIA@ Enhancement Solution. Incubated at room temperature for 5 minutes. Detected 615 nm fluorescence emission with appropriate Time-Resolved Plate Reader. (b) An in vitro assay which determines the ability of a test compound to inhibit the EGF stimulated EGFR phosphorvlation. 20 Allowed A431 cell growth in a T75 flask using standard tissue culture procedures until cells reach near confluency (~1.5x 107) cells; D-MEM, 10% FBS). Under sterile conditions dispensed 100 pl of the cell suspension per well in 96-well microplates (x cells plated per well). Incubated cells and monitor cell density until confluency is achieved with well-to-well consistency; approximately three days. Removed complete media from plate 25 wells by aspiration or manual displacement. Replaced media with 50 1d of pre-warmed serum free media per well and incubated 4 to 16 hours. Made two fold serial dilutions of inhibitor using pre-warmed D-MEM so that the final concentration of inhibitor range from 10 pM to 90 pM. Removed media from A431 cell plate. Added 100 p1 of serial diluted inhibitor into cells and incubate 1 to 2 hours. Removed inhibitor from plate wells by 30 aspiration or manual displacement. Added either serum free media for resting cells (mock) or serum free media with 100 ng/ml EGF. Used 100 pl of resting/activation media per well. Allowed incubation at 37 'C for 7.5 minutes. Removed activation or stimulation media 146 WO 2008/033749 PCT/US2007/077977 manually or by aspiration. Immediately fixed cells with 4% formaldehyde in IX PBS. Allowed incubation on bench top for 20 minutes at RT with no shaking. Washed five times with IX PBS containing 0.1% Triton X-100 for 5 minutes per Wash. Removed Fixing Solution. Using a multi-channel pipettor, added 200 pl of Triton Washing Solution (IX 5 PBS + 0.1% Triton X- 100). Allowed wash to shake on a rotator for 5 minutes at room temperature. Repeated washing steps 4 more times after removing wash manually. Using a multi-channel pipettor, blocked cells/wells by adding 100 pl of LI-COR Odyssey Blocking Buffer to each well. Allowed blocking for 90 minutes at RT with moderate shaking on a rotator. Added the two primary antibodies into a tube containing Odyssey Blocking Buffer. 10 Mixed the primary antibody solution well before addition to wells (Phospho-EGFR Tyri045, (Rabbit; 1:100 dilution; Cell Signaling Technology, 2237; Total EGFR, Mouse; 1:500 dilution; Biosource International, AHR5062). Removed blocking buffer from the blocking step and added 40 pl of the desired primary antibody or antibodies in Odyssey Blocking Buffer to cover the bottom of each well. Added 100 pl of Odyssey Blocking 15 Buffer only to control wells. Incubated with primary antibody overnight with gentle shaking at RT. Washed the plate five times with Ix PBS + 0.1% Tween-20 for 5 minutes at RT with gentle shaking, using a generous amount of buffer. Using a multi-channel pipettor added 200 pl of Tween Washing Solution. Allowed wash to shake on a rotator for 5 minutes at RT. Repeated washing steps 4 more times. Diluted the fluorescently labeled 20 secondary antibody in Odyssey Blocking Buffer (Goat anti-mouse IRDyeTM 680 (1:200 dilution; LI-COR Cat.# 926-32220) Goat anti-rabbit IRDyeTM 800CW (1:800 dilution; LI COR Cat.# 926-32211). Mixed the antibody solutions well and added 40 pl of the secondary antibody solution to each well. Incubated for 60 minutes with gentle shaking at RT. Protected plate from light during incubation. Washed the plate five times with 1x PBS 25 + 0.1% Tween-20 for 5 minutes at RT with gentle shaking, using a generous amount of buffer. Using a multi-channel pipettor added 200 1d of Tween Washing Solution. Allowed wash to shake on a rotator for 5 minutes at RT. Repeated washing steps 4 more times. After final wash, removed wash solution completely from wells. Turned the plate upside down and tap or blot gently on paper towels to remove traces of wash buffer. Scanned the plate 30 with detection in both the 700 and 800 channels using the Odyssey Infrared Imaging System (700 nm detection for IRDyeTM 680 antibody and 800 nm detection for IRDyeTM 800CW antibody). Determined the ratio of total to phosphorylated protein (700/800) using Odyssey software and plot the results in Graphpad Prism (V4.0a). Data were plotted using GraphPad 147 WO 2008/033749 PCT/US2007/077977 Prism (v4.0a) and IC50's calculated using a sigmoidal dose response curve fitting algorithm. (c) An in vitro assay which determines the ability of a test compound to inhibit HDAC enzymatic activity. 5 HDAC inhibitors were screened using an HDAC fluorimetric assay kit (AK-500, Biomol, Plymouth Meeting, PA). Test compounds were dissolved in dimethylsulphoxide (DMSO) to give a 20 mM working stock concentration. Fluorescence was measured on a WALLAC Victor 2 plate reader and reported as relative fluorescence units (RF U). Data were plotted using GraphPad Prism (v4.0a) and IC50's calculated using a siginoidal dose 10 response curve fitting algorithm. Each assay was setup as follows: Defrosted all kit components and kept on ice until use. Diluted HeLa nuclear extract 1:29 in Assay Buffer (50 mM Tris/Cl, pH 8.0, 137 mM NaCl, 2.7 mM KCI, 1 mM MgCl2). Prepared dilutions of Tnichostatin A (TSA, positive control) and tested compounds in assay buffer (5x of final concentration). Diluted Fluor de LysTM 15 Substrate in assay buffer to 100 uM (50 fold = 2x final). Diluted Fluor de LysTM developer concentrate 20-fold (e.g. 50 pl plus 950 1d Assay Buffer) in cold assay buffer. Second, diluted the 0.2 mM Trichostatin A 100-fold in the 1x Developer (e.g. 10 ul in 1 ml; final Trichostatin A concentration in the 1x Developer = 2 pM; final concentration after addition to HDAC/Substrate reaction = 1 ttM). Added Assay buffer, diluted trichostatin A or test 20 inhibitor to appropriate wells of the microtiter plate. Added diluted HeLa extract or other HDAC sample to all wells except for negative controls. Allowed diluted Fluor de LysTM Substrate and the samples in the microtiter plate to equilibrate to assay temperature (e.g. 25 or 37 0 C. Initiated HDAC reactions by adding diluted substrate (25 pl) to each well and mixing thoroughly. Allowed HDAC reactions to proceed for 1 hour and then stopped them 25 by addition of Fluor de LysTM Developer (50 i). Incubated plate at room temperature (25 C) for 10-15 min. Read samples in a microtiter-plate reading fluorimeter capable of excitation at a wavelength in the range 350- 380 nm and detection of emitted light in the range 440-460 nm. The following TABLE B lists compounds representative of the invention and their 30 activity in HDAC and EGFR assays. In these assays, the following grading was used: I ' 10 gM, 10 tM >I> 1 IM, 1 pM > III > 0.1 pM, and IV < 0.1 pM for IC 50 . 148 WO 20081033749 PCT/US2007/077977 TABLE B Compound HDAC EGFR HER2/ErB2 VEGFR 2 IIV 3 IIV 4 IIIV 5 IV IV 6 IV IV IV I 7 IIV IV 8 IIV 9 IIIV 790I IVII 80 IV IV 812 IV IV - 14 1149 WO 2008/033749 PCT/US2007/077977 82 I I 83 IV I 84 IV III 85 IV IV III 86 IV III 87 IV III 88 IV IV L 89 IV III 90 IV N/A 91 IIIV 92 IIIV 93 IIIV 94 IIV 102 IV _____ 103 1 _____ 107 111______ 112 I_ _ _ _ _ 118II_ _ _ _ _ 121 1_ _ _ _ _ 1241 ____ _ 12511_____ _ 138 I _ _ _ _ _ _ _ _ _ _ 139 I 144 II_ _ _ _ _ 145 IV IV IV 151 Iv IV IV 155 IV IV IV 161 IV III 162 IV IV III 167 IV IV III 168 IV IVII 174 1 177 IIIV IV 178II ____ _ 198 IV IV IV 199 IV IV IV 200 IV IV IV 201 IIIV IV 202II 203II 204 I 205 I _____ 206 IV IV IV 207 IV IV IV 208 IV _____ 209 IV 150 WO 20081033749 PCT/US2007/077977 A representative number of compounds were assayed against several different cell lines using the cell proliferation assay: Cell Proliferation Assay: Cancer cell lines were plated at 5,000 to 10,000 per well in 96-well flatted bottomed 5 plates with various concentration of compounds. The cells were incubated with compounds for 72 hours in the presence of 0.5% of fetal bovine serum. Growth inhibition was accessed by adenosine triphosphate (ATP) content assay using Perkin Elmer ATPlite kit. ATPlite is an ATP monitoring system based on firefly luciferase. Briefly, 25pl of mammalian cell lysis solution was added to 50 i of phenol red-free culture medium per well to lyse the cells and 10 stabilize the ATP. 2 5RI of substrate solution was then added to the well and subsequently the luminescence was measured. The results are presented below in TABLE C. In these assays, the following grading was used: I > 10 rM, 10 pM > 11 > 1 VtM, 1 pM > III > 0.1 pM, and IV < 0.1 pM for IC 50 . TABLE C Compound No. Cell Line 6 12 18 40 44 66 70 77 79 85 BreastMCF7 IV Breast MDAMB468 IV BreastSkBr3 III ColonHCT116 III III III EpidermoidA431 III LungH1703 III III II Lung H1975 III III II LungH2122 III III II LungH292 IV LungH358 III III II LungH460 III III II Lung HCC827 IV III III PancreasBxPC3 III IV III II II II II II II III Pancreas Capan1 II III II Pancreas CFPAC III III II I II II II II Pancreas HPAC II II II II I I I II Pancreas MiaPaCa2 II III II II II III II II PancreasPANC1 III III II II II I II II Prostate_22RV1 III ProstatePC3 III III 15 Figure 1 shows that compounds of the invention, such as compounds 6 and 12 are more active than erlotinib and SAHA in EFGR enzyme assay and HDAC enzyme assay. In the EFGR enzyme assay, compounds of the invention is more potent than erlotinib by 151 WO 2008/033749 PCT/US2007/077977 approximately 15-20 fold. In the HDAC enzyme assay, compounds of the invention is more potent than SAHA by approximately 5-10 fold. Figure 2 illustrates the improvement in inhibition of histone acetylation and EGFR phosphorylation by compound 12 as compared with SAHA and Erlotinib respectively. 5 Inhibition on both kinase (EGFR) and non-kinase (HDAC) cancer targets by a compound 12. Table D illustrates the potency of compounds of the invention. For example, compound 12 is more active than Erlotinib and SAHA in various cancer cell lines (IC50 in pM). Cell lines from five major types of cancer (lung, breast, prostate, colon, and pancreas) 10 responded better to compound 12 than a combination of erlotinib and SAHA. Surprisingly, the compounds of the invention are active against cell lines that are resistant to Tarceva@ and Iressa@. In these assays, the following grading was used: D> 5 tIM, 5 IM > C> 0.5 pM, 0.5 iM > B > 0.05 pM, and A < 0.05 gM for IC 50 . TABLE D Erlotinib!SAHA Tuwor, Line Twnmor Type SAHA Erlotinib Conbined MDA-MB-231 Breast aenocacinoma B D A HCT116 Colon cancer C D A MCF-7 Breast acenocarcinoma C CA MDA-MB-468 Breast adenocarcinoma C C A SKBr3 Breast carcinoma C D PC-3 Prostate adenocarcinoma C D C C Caki-1 Renal carcinoma B BA A431 Epidermoid carcnoma C C 22RV1 Prostate carcnoma 8 B Figure 3 shows examples of greater anti-proliferative activity against several different cancer cell lines. Figure 3 further shows that compounds of the invention are more potent than SAHA alone, Erlotinib alone, and SAHA and Erlotinib combined. Figure 4 displays the potency of compound 12 in induction of apoptosis in colon and 20 breast cancer cells. Compound 12 induced approximately 4-11 times more cell apoptosis as measured by increased Caspase 3&7 activity. Erlotinib was inactive at a concentration <20 pM. The high potency displayed by compound 12 over Erlotinib suggests that compounds of the invention can be used to treat tumor cells that are resistant to Erlotinib. Figures 5-10 illustrate the efficacy of compound 12 in various tumor xenograft 152 WO 20081033749 PCT/US2007/077977 models. Table E below summarizes the in vivo experiments that were carried out to give results represented in Figures 5-10. Table E Dosing Method of regimen Pre administr (on-off- treatment Model Cancer type Dosage groups ation on) tumor size A431 Epidermoid vehicle IP Once 156±57 6 mg/kg daily for mm 3 12 mg/kg 21 days 24 mg/kg 48 mg/kg H358 NSCLC vehicle IV - 2 min 7-7-5 84±23 15 mg/kg infusion mm 3 30 mg/kg 60 mg/kg H292 NSCLC vehicle IV - 2 min 7-7-5 116+23 15 mg/kg infusion mim 3 30 mg/kg 60 mg/kg BxPC3 Pancreatic vehicle IV - 2 min 7-7-2 201±53 10 mg/kg infusion mm 3 20 mg/kg 40 mg/kg PC3 Prostate vehicle IV - 2 min 7-7-5 195±50 10 mg/kg infusion mm 3 20 mg/kg 40 mg/kg HCT 116 Colon vehicle IV - 2 min 5-2-5 91±23 15 mg/kg infusion mm 3 30 mg/kg 60 mg/kg SAHA 20 mg/kg HCC827 NSCLC vehicle IV - 2 min Once 149±36 (apoptosis / 30 mg/kg infusion daily for mm3 anti- 3 days proliferation) BxPC3 Pancreatic 60 mg/kg IV - 2 min Single IV NA (apoptosis / infusion infusion anti proliferation) A representative protocol for the in vivo experiment is as followed: 5 1-10 x 106 human cancer cells were implanted subcutaneously to the athymic (nu/nu) mice. When the tumors reached about 100 mm 3 in volume, the mice were treated with the compound by tail vein infusion. Routinely 5 groups (8-12 mice per group) are 153 WO 2008/033749 PCT/US2007/077977 needed for a typical efficacy study, including one negative control, one positive control, and three testing groups for 3 dose levels of the same compound. Usually a 7-7-5 (on-off-on) regimen was used for one typical study. The tumor size was measured with an electronic caliper and body weight measured with a scale twice weekly. The tumors were removed 5 from euthanized mice at the end of the study. One half of each tumor was frozen in dry ice and stored at -80' C for PK or Western blot analysis. The other half was fixed with formalin. The fixed tissues were processed, embedded in paraffin and sectioned for immunohistochemistry staining. 10 Protocol for Radioisotope assay for HER2 10 nM HER2 and 0.1 mg/mi polyEY were placed in the reaction buffer and 2 mM MnCl 2 , I piM ATP and 1% DMSO final were added. The reaction mixture was ncubated for 2 hours at room temperature. The conversion rate of ATP was 22%. HER2 (Accession number: GenBank X03363) is characterized as follows: 15 N-terminal GST-tagged, recombinant, human HER2 amino acids 679-1255, expressed by baculovirus in Sf9 insect cells. Purity >90% by SDS PAGE and Coomassie blue staining. MW = 91.6kDa. Specific Activity of 40 U/mg, where one unit of activity is defined as Inmol phosphate incorporated into 30 ug/ml Poly (Glu:Tyr)4:1 substrate per minute at 30'C with a final ATP concentration of 100M. Enzyme is in 25 mM Tris-HCI, pH 8.0, 100 mM 20 NaCl, 0.05% Twcen-20, 50% glycerol, 10 mM reduced glutathione, and 3 mM DTT. References: 1. Meyer, M. etal., EMBO J. 18, 363-374 (1999) 2. Rahimi, N. etal., J. Biol Chem 275, 16986-16992 (2000) Compounds of the invention are found to be active against various kinases. For 25 example, Table F shows inhibition of compound 12 in a panel of kinase assays. Furthermore, Compound 12 is much more active than Erlotinib in Her-2 assay. 154 WO 2008/033749 PCT/US2007/077977 TABLE F Assays Concentrations (pM) fnhibition (%) Abi Kinase 5 57 FGFR2 Kinase 5 73 FLT-3 Kinase 5 85 VEGFR2 Kinase 5 64 Lck Kinase 5 56 Lyn Kinase 5 95 Ret Kinase 5 93 Her-2 Compound 12 IC50 = 188 nM Erlotinib IC50 = 1473 nM Example 73: Preparation of Captisol formulation of compound 12 5 A. Preparation of25, 30. 40, 50 and 60 mg/mli solutions of compound 12 in 30% Captisol (i) With tartaric acid A 30% Captisol formulation was prepared by adding 2.7 ml water to a vial containing 0.9 g Captisol. The mixture was then mixed on a vortexer to give ~3ml of a clear solution. 10 In order to prepare a formulation of 25 mg/ml solution of compound 12, 1 ml of the 30% Captisol solution was added to a vial containing 25 mg of compound 12 and 8.6 mg tartaric acid and the resulting mixture was mixed on a vortexer or sonicated at 3 OC for 15 to 20 minutes to give a clear yellowish solution. The resulting solution is stable at room temperature. 15 In order to prepare a formulation of 30 mg/ml solution of compound 12, 1 ml of the 30% Captisol solution was added to a vial containing 30 mg of compound 12 and 10.4 mg tartaric acid (1.0 eq) at room temperature. In order to prepare a formulation of 40 mg/ml solution of compound 12, 1 ml of the 30% Captisol solution was added to a vial containing 40 mg of compound 12 and 17.9 mg 20 tartaric acid (1.3 eq) at 36 0 C. In order to prepare a formulation of 50 mg/ml compound 12 in Captisol, I ml of 30% Captisol was added to 50 mg compound 12, 22.5 mg tartaric acid (1.3 eq) at 37C. In order to prepare a formulation of 60 mg/ml compound 12 in Captisol, the 30% Captisol was added to a vial containing 60 mg compound 12 and 26.9 mg tartaric acid (1.3 155 WO 20081033749 PCT/US2007/077977 eq) at 36'C. The solution was diluted in 1X water and 2X D5W. The diluted solution is stable at room temperature for >1 2h. (ii) With citric acid In order to prepare a formulation of 25 mg/ml solution of compound 12, 1 ml of the 5 30% Captisol solution was added to a vial containing 25 mg of compound 12 and 11.1 mg citric acid (1.0 eq) and the resulting mixture was mixed on a vortexer or sonicated at room temperature for 15 to 20 minutes to give a clear yellowish solution. (iii) With hydrochloric acid In order to prepare a formulation of 25 mg/ml solution of compound 12, 1 ml of the 10 30% Captisol solution was added to a vial containing 25 mg of compound 12 and 57.5 pl hydrochloric acid (1.0 cq) and the resulting mixture was mixed on a vortexer or sonicated at room temperature for 15 to 20 minutes to give a clear yellowish solution. (iiii) With sodium salt In order to prepare a formulation of 7.5 mg/ml solution of compound 12, 1 ml of the 15 30% Captisol solution was added to a vial containing 7.5 mg of compound 12 sodium salt and the resulting mixture was mixed on a vortexer or sonicated at room temperature for 15 to 20 minutes to give a clear yellowish solution. B. Filtration of the Solution 20 The formulations of compound 12 from A (i) was filtered through a 0.2-pm presterilized filter with >98% recovery. C. Preparation of a lyophilisate The formulations of compound 12 (25 mg/ml) from A (i) and A (iii) were 25 lyophilized to form lyophilisate as a yellow powder. The lyophilisate resulted from A (i) formulation was chemically stable at following temperatures, -20"C, room temperature, and 40'C for at least 2 weeks. It can be stored at 4'C for greater than 2 weeks without decomposition. The lyophilisate resulted from A (iii) was stable at -20*C for at least two weeks. 30 D. Dilution Study The formulations of compound 12 from A (i) were diluted with D5W (10-, 20-, and 50-fold) and were chemically stable and remained in solution without precipitation (>48 hours). 156 WO 2008/033749 PCT/US2007/077977 The formulations of compound 12 from A (ii), A (iii) and A (iiii) were diluted with D5W (10-fold) and remained in solution without precipitation (>12 hours). 5 Example 74: Characteristics of sodium, hydrochloride, citric acid and tartaric acid salts or complexes of compound 12 formulated in CAPTISOL Sodium, hydrochloride, citrate and tartrate salts of a test compound of Formula I were prepared in 30% CAPTISOL solutions and were studied for the following: Table G shows the physiochemical as well as pharmacokinetic (PK) and 10 pharmacodynamic (PD) properties of sodium, hydrochloride, citric acid and tartaric acid salts of Compound 12. TABLE G Sodium HCI Citric Acid Tartaric Acid Solubility 7.5 mg/ml 25 mg/ml 25 mg/ml 60 mg/ml pH 10-11 2-3 4-5 3-4 IV Tissue High High Low High Dilution with >lox >lox >lox >50x D5W Chemical stability >12 h >12 h >12 h >12 h in diluted solution 2-week chemical ND -20 0 C ND -20 0 C, RT, stability in 40 0 C lyophilisate Deliverable 220-250 >750 mg > 750 mg > 1800 mg highest daily dose mg in humans Example 75: Comparison of anti-tumor activity of composition of compound 12 in 30% 15 CAPTISOL and erlotinib, a prototype EGFRi in A549 NSCLC xenograft model Administration of compound 12 in 30% CAPTISOL attenuated tumor growth in the NSCLC xenograft model. As shown in FIGs. 11A, after 24 hours, animals treated with compound 12 showed a 150% increase in tumor size whereas animals treated with vehicle showed about a 240% change in tumor size. As shown in FIG. 1 IB, treatment of animals 20 with Erolotinib did not significantly affect tumor size as compared to control. 157 WO 2008/033749 PCT/US20071077977 Example 76: Effect of composition of compound 12 in 30% Captisol in HPAC pancreatic cancer cel1s 120 mg/kg of compound 12 in 30% CAPTISOL, 50 mg/kg erlotinib or vehicle were 5 administered to animals daily and change in tumor size over time (days) was measured. As shown in FIG. 12A, administration of 120 mg/kg compound 12 in 30% CAPTISOL (iv/ip) resulted in greater attenuation of tumor growth than either erlotinib (po) or vehicle. Pharmacokinetic studies in mice, rats, and dogs 10 The experimental methods used for Examples 77-83 are described below. Animals: Mice (CD-1, male, 25-30 g), rats (Spraque Dawle, 260-300 g) and dogs (Beagles, male, 9-11 kg) were used for the PK studies. Animals were provided pelleted food and water ad libitum and kept in a room conditioned at 23±1'C, humidity of 50 - 70%, and a 12 hour light/12-hour dark cycle. 15 Drug Preparation and Administration. Compound 12 was dissolved in 30% CAPTISOL with equal molar concentration of tartaric acid or HCl or citric acid, or NaOH. Compound was administered via an intravenous (iv) infusion. Conditions for iv infusion for each animal are shown below: Mouse: 20 mg/kg and 60 mg/kg for 2 min i.v. infusion 20 Rat: 20 mg/kg for 5 min i.v. infusion Beagle: 25 mg/kg for 30 mi i.v. infusion. Blood and tissue Sample Collection. Blood was collected into tubes containing sodium heparin anticoagulant at various time points. The plasma was separated via centrifugation 25 and stored in -40'C before analysis. Plasma Sample Extraction. Plasma samples were prepared by protein precipitation. An internal standard was added into plasma samples. A 50 pl of plasma was combined with 150 pl of acetonitrile, vortexed, and centrifuged for 10 min at 10000 rpm. The supernatant was then injected onto LC/MS/MS. 30 Samples were compared to standards made in plasma. These standards were prepared by serial dilution. An internal standard was added into the plasma with standard. 158 WO 2008/033749 PCT/US2007/077977 Tissue Sample Extraction Lung and colon samples (20-200 mg) were used for extraction. Tissues were homogenized in 0.8 ml water. An internal standard was added into the tissue homogenates. The homogenates were extracted with 1-ml ethyl acetate for three 5 times. After evaporation, the residual was reconstituted in 0.1 ml acetonitrile for LC/MS/MS assay. LCA/MS/MS Analytical Methods. LC Conditions are shown below: LC Instrument: Agilent HPLC 1100 Series 10 Autosampler: Agilent G1367A Autosampler Analytical Column: YMC Pro C18 S3 (3[L, 2.0*50 mm, 120 A) Guard Column: YMC Pro C18 S3 Guard Column (3p1, 2.0* 10 mm, 120 A) Column Temp: in ambient 15 Mobile Phase: A: acetonitrile:water:formic acid (5:95:0.1, v/v/v) B: acetonitrile:water:formic acid (95:5:0.1, v/v/v) LC Gradient Program 0~-min: mobile phase A: 100% 1-2.5min: mobile phase A: 100% to 20% 2.5-3min: mobile phase A: 20% 20 3-4min: mobile phase A: 20% to 100% Flow Rate: 200 1tl/min Autosampler Temp: in ambient Injection Volumn: 5 il Mass Spectrometer conditions are shown below: 25 Instrument: PE Sciex API 3000 Interface: Turbo Ion Spray (TIS) Polarity: Positive Ion Scan: Multiple Reaction Monitoring (MRM) 30 Single or multiple dosing toxicity study in mice and rats The experimental methods used for the toxicity study below are described as follows: Experiment design: 159 WO 20081033749 PCT/US2007/077977 1. Single dosing MTD in mice a. CD-1 mice, male, 24 -26 gram b. Dosing at 0, 50, 100, 200, 400 mg/kg, iv infusion 2 min c. 8 mice per group 5 2. Single dosing MTD in rats a. Sprague Dawley, male and female, 240-260 gram b. Dosing at 0, 25, 50, 100, 200 mg/kg, iv infusion 5 min c. 6 rats per group (3 male and 3 female) 3. 7-day -multiple dosing MTD in mice 10 a. CD-I mice. male, 24 -26 gram b. Dosing at 0, 50, 100, 200 mg/kg/d ip c. 6 mice per group d. Blood and organs will be collected 2 hr after last dosing on Day 7 for hematology 15 4. 7-day multiple dosing MTD in rats a. Sprague Dawley, male and female, 220-250 gram b. Dose , 25, 50, 100, 200 mg/kg/d) iv infusion 5 min c. 6 per group (3 male and 3 female) d. Blood and organs will be collected 2 hr after last dosing on Day 7 for 20 hematology Compound preparation The compound was dissolved in 30% Captisol with equal molar concentration of tartaric acid. The stock solution: 25 mg/mI Tartaric form in 30% Captisol, 1 ml/vial store at 25 -40'C. For example, 1000 mg compound, 345 mg tartaric acid (0.345 mg tartaric acid per mg compound) and 40 ml 30% Captisol or 1000 mg compound, 2.3 ml IN HCI (2.3 ul IN HCI per mg compound), 12 gram Captisol, Add water to 40 ml. Stock solution is diluted with 30% CAPTISOL before use. 30 Example 77: Pharmacokinetics of test salts in plasma, lung and colon after intravenous administration 20 mg/kg of hydrochloride, citrate, sodium and tartrate salts of compound 12 in 30% CAPTISOL was administered intravenously to mice in order to determine the concentration 160 WO 2008/033749 PCT/US2007/077977 (ng/ml) over time (hours) of compound 12 after intravenous (iv) administration in plasma, lung and colon. The results of these studies are shown in FIG. 13. As shown there, similar plasma and tissue pharmacokinetics was observed for the sodum, hydrochloride and tartrate salts. 5 Example 78: Pharmacokinetic study of compound 12 formulation in mice 20 mg/kg and 60 mg/kg of a hydrochloride salt of compound 12 in 30% CAPTISOL was administered intravenously (iv) and intraperitoneally (ip) to mice and the half life (t 1/2), maximal observed concentration (Cmax) and area under the curve (AUC) were 10 determined. As shown in Table H below, the concentration of compound 12 is dose proportional when administered intravenously but not intraperitoneally. The half-life of compound 12 in tissue is greater than that in plasma. TABLE H Plasma Lung Colon 15 20 mgkg 60 mg/kg 20 mgkg 60 mg/kg 20 mgkg 60 mg/k IV Dose T 1/2 (hr) 0.2 0.3 3.9 1.9 1.7 2. Cmax (uM) 27.7 61.7 15.2 96.9 8.5 29.z AUC (h*ng/ml) 715 3124 1571 8313 5529 13472 IP Dose T 1/2 (hr) 0.26 0.51 2.2 3.5 NA NA 20 Cmax (uM) 8.5 14.4 7.8 11.6 NA NA AUC (h*ng/ml) 3751 5721 4433 8309 NA NA Example 79: Pharmacokinetic study of compound 12 formulation in rats 20 mg/kg and 60 mg/kg of a hydrochloride salt of compound 12 in 30% CAPTISOL was administered (iv) to rats and the concentration (ng/ml) of the compound was measured 25 in plasma over thirty hours. As shown in FIG. 14, the concentration of compound 12 in the plasma of the rat was proportional to the dose of compound 12 administered. Example 80: Single dose IV toxicity study in mice with the compound 12 formulation A single dose of compound 12 (25, 50, 100, 200 or 400 mg/kg) in 30% CAPTISOL 30 was administered (iv) to mice and change in body weight was measured over nine day to assess toxicity of the various doses of compound 12. As shown in FIG. 15, administration of up to 200 mg/kg of compound 12 did not result in a significant change in body weight. 161 Example 81: Seven day repeat IP toxicity study in mice using compound 12 formulation Repeated dosing of compound 12 over seven days (25, 50, 100, 200 or 400 mg/kg) in 30% CAPTISOL was administered (ip) to mice and change in body weight was measured over seven days. As shown in FIG. 16, repeated administration of up to 100 mg/kg of compound 12 did not result in a significant change in body weight. Example 82: Single does IV toxicity study in rats using compound 12 formulation A single dose of compound 12 (25, 50, 100 or 200 mg/kg) in 30% CAPTISOL was administered (iv) to rats and change in body weight was measured over eight days to assess toxicity of the different doses of compound 12. As shown in FIG. 17, administration of up to 200 mg/kg did not result in a significant change in body weight. The patent and scientific literature referred to herein establishes the knowledge that is available to those with skill in the art. All United States patents and published or unpublished United States patent applications cited herein are incorporated by reference. All published foreign patents and patent applications cited herein are hereby incorporated by reference. All other published references, documents, manuscripts and scientific literature cited herein are hereby incorporated by reference. While this invention has been particularly shown and described with references to preferred embodiments thereof, it will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the scope of the invention encompassed by the appended claims. Throughout this specification and the claims which follow, unless the context requires otherwise, the word "comprise", and variations such as "comprises" and "comprising", will be understood to imply the inclusion of a stated integer or step or group of integers or steps but not the exclusion of any other integer or step or group of integers or steps. The reference to any prior art in this specification is not, and should not be taken as, an acknowledgement or any form of suggestion that the prior art forms part of the common general knowledge in Australia. 162

Claims (21)

1. A compound of Formula (VIII), 0 HN HN Y n , R R21 R4 N (VIII) or a pharmaceutically acceptable salt or solvate thereof, wherein Ra and Rb are hydrogen or are taken together with the carbon atom they attached to form -C(O)-; n is 0-9; R 20 , R 21 , R 22 are independently selected from hydrogen, hydroxy, amino, halogen, alkoxy, alkylamino, dialkylamino, CF 3 , CN, NO 2 , sulfonyl, acyl, aliphatic, substituted aliphatic, aryl, substituted aryl, heteroaryl, substituted heteroaryl, heterocyclic, and substituted heterocyclic; X1 is 0, S or NH; Q is absent or substituted or unsubstituted alkyl; Y and R 7 are absent; R 4 is selected from hydrogen, hydroxy, amino, halogen, substituted or unsubstituted alkoxy, substituted or unsubstituted alkylamino, substituted or unsubstituted dialkylamino, CF 3 , CN, NO 2 , sulfonyl, acyl, aliphatic, and substituted aliphatic; and R 8 is hydrogen or aliphatic.

2. The compound of claim 1 wherein R 8 is hydrogen.

3. The compound of claim 1 wherein Q is absent.

4. The compound of claim 1 represented by the formula 163 O HN Ar HO 0 H m R4 N or a pharmaceutically acceptable salt or solvate thereof, wherein: Ar is selected from 3-chloro-4-fluorophenyl and 3-ethynylphenyl: R 4 is selected from hydrogen, methoxy and 2-methoxyethoxy; and m is from 1 to 6.

5. The compound of claim 4 wherein m is 6.

6. The compound of claim 1 represented by the formula O HN Q-Ar HO 0 N I N H 6 R4N or a pharmaceutically acceptable salt or solvate thereof; wherein: Q is absent and Ar is 3-chloro-4-fluorophenyl, 3-ethynylphenyl, 3-ethynyl-4 fluorophenyl, 3-chloro-4-(3-fluorophenylmethoxy)phenyl or 3 fluorophenylmethoxyphenyl; Q is -CH 2 - and Ar is phenyl, 3-chloro-4-fluorophenyl, 3-ethynylphenyl, 4-fluorophenyl, 3,4-difluorophenyl, or 3-bromophenyl; Q is -CH 2 -CH 2 - and Ar is phenyl; or Q is -CH(CH 3 )- and Ar is phenyl, 4-fluorophenyl, 4-chlorophenyl, 4-methoxyphenyl; and R 4 is methoxy. 164

7. The compound of claim 1 represented by the formula HNAr HNH H H N N HO 6 , 0 0 or a pharmaceutically acceptable salt or solvate thereof; wherein: Ar is 3-chloro-4-fluorophenyl or 3-ethynylphenyl; and R 4 is methoxy or 2-methoxyethoxy.

8. A compound of the formula HN H HO'N O N HN or a pharmaceutically acceptable salt or solvate thereof.

9. A compound according to Claim 1 selected from the compounds delineated in the table below or a pharmaceutically acceptable salt thereof: Compound # Structure F HN CI HO, 0J-, H ODC-N F HN CI HO N 0 165 F O HN CI HO, 0 NHNNC H NF 4 O HN CI HOO _ _ _ _ _O N F 5 0 HN CI HO- N NH ____HO__O O N F 6 H HN C HO N O 0N 0 N HO HN < HO' N H O N 1 0 HN HO- NJ O N HI 0O N HO O O66 00 90 HN HO- , <N H 10 HHN H 16 0 12 NHN HO N 0 0 & O N F 13 0 HN CI HO ~ O NNC HO, N O H F 14 H HN CI 0 HOO 15 0 HN CI 16HO-N O HNN 163 HN CI HONO 19 0 HN Zci HO O 167 17 0 H N c IZ HO 0 N 116 N 20 H HN HON O O N 21 0 H HON 0 H N24HN HO' N N HON N H 24 H H HO-N F 25 0 HN a ci HO 0 26 HO' N- N 0 F 27 0 HNa cI HO,~ N' ' 0 H 168 F 28 H HN cI F 29 HN cI HO, Nl N -N H 30 H H HO' N 0 Dot" 0 N 0 HN 31 0H HO, 0 j N0 H 32 H H HO N -,0 ~0 N 330 HN H00 34 H N HI HO' N- I -, -; 35 HN N HO,. N 0 H ll, D - N 169 36H HN HO>N 0 H N c 37 HO, N NN H I 0 0O N. F HH H 38HO' Ny _- N H 00 )CD KYF 0H H N c 1 40HO.N N N H 0 0 N H H N cZ I 40 HO N N H HN 41 ~HO, 1 D NN HN H H 43HO. N N HH 0 N 170 44 H HN' HO- N- N H 50HN HO, N H KYF H HN"a~OI 67 HO,. N NN HNdC ~0 F H HI 68 HO' N-_. NN 00 N ~oll F 69HO NN * N H II 0 N. 00 HO ! "H HNac H 0 H~N ~0 171 SH HN HON-r N HO' 0 0 73 HO..H H O N HH NO N 7 3 HO H CH O N O HC 74 HO N " N HCON HH Oj HO HN 0F 75 O N CH H ~CH 00H HF F 76 H "O0 -IH HO N O HsC CH, 770 HN N O "HN H CH 78 H HN HO 0 -N! CH, 79 H NN 80 HH I 0 FC.. 0 1 o N CH, 81 HN F 0 HC 0 N 172 CH, 82 HHN O HC. C N CH, 83 HN O HCN 84 OH HN HN O O H~C O 0 N ~ 85 H HO' N~_ 0 0 OH HN 86 HN0 HN --- O -N F O HC N S F OH HN 87 H - .. Kk O HaC-0 N 88 HHN HO NF O H 0C N OH HN 89 HN 0 O H3C N Br HNH 90 HO CH F 94HN CI HO 0 HG O N 149 H HN C HO1 7 H 173 F 151 H HN CI HO'N H0 N faF 153 HO O CI aF 155 H HN CI H O , NO H HO N N aF 155 H HN I 0 OD: N 'F 198 HFN- C o ,, <CN 199 HC 200 OH 206 H >CH HO HN 0 HCGO N HH 207 H H CH 208 HN H HOCH

10. A pharmaceutical composition comprising as an active ingredient a compound of Claim 1 and a pharmaceutical acceptable carrier.

11. A method of treating an EFGR-tyrosine kinase related disease or disorder in a subject in need thereof, the method comprising administering to the subject a therapeutically effective amount of the pharmaceutical composition of Claim 10. 174

12. The method of Claim 11, wherein said EGFR-tyrosine kinase related disease or disorder is a cell proliferative disorder.

13. The method of Claim 12, wherein said cell proliferative disorder is selected from the group consisting of papilloma, blastoglioma, Kaposi's sarcoma, melanoma, non-small cell lung cancer, ovarian cancer, prostate cancer, squamous cell carcinoma, astrocytoma, head cancer, neck cancer, bladder cancer, breast cancer, lung cancer, colorectal cancer, thyroid cancer, pancreatic cancer, gastric cancer, hepatocellular carcinoma, leukemia, lymphoma, Hodgkin's disease and Burkitt's disease.

14. A method of treating an HDAC-mediated disease comprising administering to a subject in need thereof a pharmaceutical composition of Claim 10.

15. A method of treating a disease mediated by both EGFR-tyrosine kinase and HDAC comprising administering to a subject in need thereof a pharmaceutical composition of Claim 10.

16. A pharmaceutical composition comprising as an active ingredient a compound of Claim 8 and a pharmaceutically acceptable carrier.

17. A method of treating an EFGR-tyrosine kinase related disease or disorder in a subject in need thereof, the method comprising administering to the subject a therapeutically effective amount of the pharmaceutical composition of Claim 16.

18. The method of Claim 17, wherein said EGFR-tyrosine kinase related disease or disorder is a cell proliferative disorder.

19. The method of Claim 18, wherein said cell proliferative disorder is selected from the group consisting of papilloma, blastoglioma, Kaposi's sarcoma, melanoma, non-small cell lung cancer, ovarian cancer, prostate cancer, squamous cell carcinoma, astrocytoma, head cancer, neck cancer, bladder cancer, breast cancer, lung cancer, colorectal cancer, thyroid cancer, pancreatic 175 cancer, gastric cancer, hepatocellular carcinoma, leukemia, lymphoma, Hodgkin's disease and Burkitt's disease.

20. A method of treating an HDAC-mediated disease comprising administering to a subject in need thereof a pharmaceutical composition of Claim 16.

21. A method of treating a disease mediated by both EGFR-tyrosine kinase and HDAC comprising administering to a subject in need thereof a pharmaceutical composition of Claim 16. 176