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AU2003239129A1 - Methods and compositions for dna manipulation - Google Patents

Methods and compositions for dna manipulation

Info

Publication number
AU2003239129A1
AU2003239129A1 AU2003239129A AU2003239129A AU2003239129A1 AU 2003239129 A1 AU2003239129 A1 AU 2003239129A1 AU 2003239129 A AU2003239129 A AU 2003239129A AU 2003239129 A AU2003239129 A AU 2003239129A AU 2003239129 A1 AU2003239129 A1 AU 2003239129A1
Authority
AU
Australia
Prior art keywords
compositions
methods
dna manipulation
manipulation
dna
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2003239129A
Other versions
AU2003239129A8 (en
Inventor
Jurate Bitinaite
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
New England Biolabs Inc
Original Assignee
New England Biolabs Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=29255337&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=AU2003239129(A1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by New England Biolabs Inc filed Critical New England Biolabs Inc
Publication of AU2003239129A8 publication Critical patent/AU2003239129A8/en
Publication of AU2003239129A1 publication Critical patent/AU2003239129A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
AU2003239129A 2002-04-12 2003-04-04 Methods and compositions for dna manipulation Abandoned AU2003239129A1 (en)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US37235202P 2002-04-12 2002-04-12
US60/372,352 2002-04-12
US37267502P 2002-04-15 2002-04-15
US60/372,675 2002-04-15
US42101002P 2002-10-24 2002-10-24
US60/421,010 2002-10-24
PCT/US2003/010296 WO2003087301A2 (en) 2002-04-12 2003-04-04 Methods and compositions for dna manipulation

Publications (2)

Publication Number Publication Date
AU2003239129A8 AU2003239129A8 (en) 2003-10-27
AU2003239129A1 true AU2003239129A1 (en) 2003-10-27

Family

ID=29255337

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2003239129A Abandoned AU2003239129A1 (en) 2002-04-12 2003-04-04 Methods and compositions for dna manipulation

Country Status (5)

Country Link
US (3) US7435572B2 (en)
EP (2) EP2455488B1 (en)
JP (1) JP4663988B2 (en)
AU (1) AU2003239129A1 (en)
WO (1) WO2003087301A2 (en)

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US20040219584A1 (en) * 2000-10-20 2004-11-04 Xu Shuang-Yong Methods for altering the cleavage specificity of a type IIG restriction endonuclease
US6958217B2 (en) * 2001-01-24 2005-10-25 Genomic Expression Aps Single-stranded polynucleotide tags
US20050136462A1 (en) 2003-12-19 2005-06-23 New England Biolabs, Inc. Method for engineering nicking enzymes
US20080268507A1 (en) * 2004-05-25 2008-10-30 Airbus Deutschland Gmbh Recombinant Dna Nicking Endonuclease and Uses Thereof
US7820424B2 (en) 2004-07-22 2010-10-26 New England Biolabs, Inc. Nicking endonuclease methods and compositions
WO2006099604A2 (en) * 2005-03-16 2006-09-21 Compass Genetics, Llc Methods and compositions for assay readouts on multiple analytical platforms
GB0610045D0 (en) * 2006-05-19 2006-06-28 Plant Bioscience Ltd Improved uracil-excision based molecular cloning
US20090036325A1 (en) * 2007-05-25 2009-02-05 Applera Corporation Directed assembly of amplicons to enhance read pairing signature with massively parallel short read sequencers
WO2009000281A1 (en) * 2007-06-22 2008-12-31 Canvax Biotech, S.L. Methods for directional cloning of amplified nucleic acids and uses thereof
ES2364920T3 (en) * 2008-10-08 2011-09-16 Icon Genetics Gmbh CLEAN CLONING PROCESS.
US8017328B2 (en) * 2008-11-21 2011-09-13 Agilent Technologies, Inc. Genome partitioning using a nicking endonuclease
WO2011030154A1 (en) * 2009-09-12 2011-03-17 Zoragen Biotechnologies Llp Detection of quantitative genetic differences
WO2012012037A1 (en) 2010-07-19 2012-01-26 New England Biolabs, Inc. Oligonucleotide adaptors: compositions and methods of use
DE102010056289A1 (en) 2010-12-24 2012-06-28 Geneart Ag Process for the preparation of reading frame correct fragment libraries
KR101306988B1 (en) 2011-03-16 2013-09-10 연세대학교 산학협력단 Assembly Methods of Multiple Target Loci to a Single Nucleotide Sequence
WO2012166647A1 (en) * 2011-05-27 2012-12-06 Life Technologies Corporation Methods for manipulating biomolecules
WO2013033528A1 (en) 2011-09-01 2013-03-07 Jennifer Ong Compositions and methods relating to variant dna polymerases and synthetic dna polymerases
EP3760733A1 (en) 2013-10-17 2021-01-06 Takara Bio USA, Inc. Methods for adding adapters to nucleic acids and compositions for practicing the same
AU2014348224A1 (en) 2013-11-18 2016-06-02 Takara Bio Usa, Inc. Degradable adaptors for background reduction
WO2015094861A1 (en) 2013-12-17 2015-06-25 Clontech Laboratories, Inc. Methods for adding adapters to nucleic acids and compositions for practicing the same
GB201516348D0 (en) * 2015-09-15 2015-10-28 Labgenius Ltd Compositions and methods for polynucleotide assembly
US10155939B1 (en) 2017-06-15 2018-12-18 New England Biolabs, Inc. Method for performing multiple enzyme reactions in a single tube
GB2574197B (en) 2018-05-23 2022-01-05 Oxford Nanopore Tech Ltd Double stranded polynucleotide synthesis method and system.
GB201811810D0 (en) * 2018-07-19 2018-09-05 Oxford Nanopore Tech Ltd Method
GB201811811D0 (en) 2018-07-19 2018-09-05 Oxford Nanopore Tech Ltd Method
US12188011B2 (en) 2018-10-19 2025-01-07 New England Biolabs, Inc. Compositions and methods for improved in vitro assembly of polynucleotides
US12435332B2 (en) 2018-10-19 2025-10-07 New England Biolabs, Inc. Ordered assembly of multiple DNA fragments
US20210383891A1 (en) 2018-10-19 2021-12-09 New England Biolabs, Inc. Improved Ordered Assembly of Multiple DNA Fragments
AU2020206999B2 (en) 2019-01-07 2025-10-02 Agilent Technologies, Inc. Compositions and methods for genomic dna and gene expression analysis in single cells
CA3166578A1 (en) 2019-12-31 2021-07-08 Singular Genomics Systems, Inc. Polynucleotide barcodes for long read sequencing
EP4263827A2 (en) 2020-12-15 2023-10-25 New England Biolabs, Inc. Compositions and methods for improved in vitro assembly of polynucleotides
EP4251770A4 (en) 2021-02-08 2024-05-29 Singular Genomics Systems, Inc. Methods and compositions for sequencing complementary polynucleotides
EP4095254A3 (en) 2021-05-27 2022-12-14 New England Biolabs, Inc. Fragmentation of dna
CN114457146B (en) * 2022-01-28 2024-11-19 赛纳生物科技(北京)有限公司 A method for double-end amplification sequencing on a solid phase medium surface

Family Cites Families (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5333675C1 (en) 1986-02-25 2001-05-01 Perkin Elmer Corp Apparatus and method for performing automated amplification of nucleic acid sequences and assays using heating and cooling steps
US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4683195A (en) * 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US5683896A (en) 1989-06-01 1997-11-04 Life Technologies, Inc. Process for controlling contamination of nucleic acid amplification reactions
US4983195A (en) * 1990-01-04 1991-01-08 Corning Incorporated Method of making fiber optic coupler with longitudinal protrusions
WO1992018521A1 (en) 1991-04-10 1992-10-29 Life Technologies, Inc. Method for amplifying and altering an rna sequence
US5137814A (en) * 1991-06-14 1992-08-11 Life Technologies, Inc. Use of exo-sample nucleotides in gene cloning
US5229283A (en) * 1991-06-14 1993-07-20 Life Technologies, Inc. Use of exo-sample nucleotides in gene cloning
US5556771A (en) * 1995-02-10 1996-09-17 Gen-Probe Incorporated Stabilized compositions of reverse transcriptase and RNA polymerase for nucleic acid amplification
WO1997003210A1 (en) * 1995-07-11 1997-01-30 FORFÁS (trading as BIORESEARCH IRELAND) Glycosylase mediated detection of nucleotide sequences at candidate loci
CA2249717A1 (en) 1996-03-18 1997-09-25 Richard K. Wilkosz Target nucleic acid sequence amplification
DE19812103A1 (en) * 1998-03-19 1999-09-23 Bernauer Annette Iteratively synthesizing nucleic acid from oligonucleotides which contain cleavable sites, useful for preparation of genes, ribozymes etc.
JP2002512044A (en) * 1998-04-22 2002-04-23 エンタープライズ・アイルランド A method for characterizing a nucleic acid molecule, comprising generating an extendable upstream DNA fragment resulting from nucleic acid cleavage at an basic site.
US6048696A (en) * 1998-05-13 2000-04-11 Epicentre Technologies Corporation Method of identifying nucleic acid molecules
US6358712B1 (en) * 1999-01-05 2002-03-19 Trustee Of Boston University Ordered gene assembly
JP4669614B2 (en) * 1999-02-22 2011-04-13 ソレクサ・インコーポレイテッド Polymorphic DNA fragments and uses thereof
US6191267B1 (en) 2000-06-02 2001-02-20 New England Biolabs, Inc. Cloning and producing the N.BstNBI nicking endonuclease
GB0018120D0 (en) * 2000-07-24 2000-09-13 Fermentas Ab Nuclease
US6350580B1 (en) * 2000-10-11 2002-02-26 Stratagene Methods for detection of a target nucleic acid using a probe comprising secondary structure
CA2427319A1 (en) 2000-10-30 2002-12-27 Gene Logic, Inc. Partially double-stranded nucleic acids, methods of making, and use thereof
US6660475B2 (en) * 2000-12-15 2003-12-09 New England Biolabs, Inc. Use of site-specific nicking endonucleases to create single-stranded regions and applications thereof
WO2002059357A2 (en) 2001-01-24 2002-08-01 Genomic Expression Aps Assay and kit for analyzing gene expression
US6958217B2 (en) * 2001-01-24 2005-10-25 Genomic Expression Aps Single-stranded polynucleotide tags
US6395523B1 (en) 2001-06-01 2002-05-28 New England Biolabs, Inc. Engineering nicking endonucleases from type IIs restriction endonucleases
EP1281757A1 (en) * 2001-07-31 2003-02-05 Direvo Biotech AG Method for the production of nucleic acids consisting of stochastically combined parts of source nucleic acids
WO2003055975A2 (en) 2001-08-23 2003-07-10 New England Biolabs, Inc. A method for engineering strand-specific, sequence-specific, dna-nicking enzymes

Also Published As

Publication number Publication date
JP2006504399A (en) 2006-02-09
US20090042258A1 (en) 2009-02-12
EP1501950A2 (en) 2005-02-02
JP4663988B2 (en) 2011-04-06
US20110262973A1 (en) 2011-10-27
EP2455488A3 (en) 2012-08-15
EP2455488A2 (en) 2012-05-23
AU2003239129A8 (en) 2003-10-27
US8440401B2 (en) 2013-05-14
WO2003087301A8 (en) 2005-03-24
EP1501950A4 (en) 2006-03-15
EP2455488B1 (en) 2017-06-07
WO2003087301A3 (en) 2004-12-09
US7435572B2 (en) 2008-10-14
US20030194736A1 (en) 2003-10-16
WO2003087301A2 (en) 2003-10-23

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Legal Events

Date Code Title Description
MK6 Application lapsed section 142(2)(f)/reg. 8.3(3) - pct applic. not entering national phase